Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	602,773	T→C	T128T (ACA→ACG)	unknown ←	Aerobic respiration control response regulator ArcA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	602,773	0	T	C	100.0%	254.3 / NA	75	T128T (ACA→ACG)	unknown	Aerobic respiration control response regulator ArcA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (36/39);  total (36/39)

CTTCAAAATGTTTACGAATTCGACGAATGGTCACATCCACCGTACGATCCTGCGGTTTGAGCTCGCGTCCGGTCATTTTCTTCAATAATTCTTCACGGGTTTGAATTTTGCCCGGATTCTCGCAGAAATGTAACATGGCGCGGAACTCACTACGCGGCAATTTGCGCTCCTCGCCTTCCGGCGTAACCAACGTGCGACTGTTTAAATCCAGTGTCCAGCCGTTAAATTTATATTGTTCCACATGGTGTGTGCGTTGATTCTCCTGCATGGTACGCTGCAACAGGTTTCTGGCGCGAATCGTTAATTCACGCGGGTTGAAAGGTTTGGTGATGTAATCATCGGCACCAATTTCCAAGCCTAGAATTTTATCCACTTCGTTATCTCGACCGGTTAAAAACATCAGTGCAATGTCCGCATTTTCACGCAATTCACGGGCAAGCATTAAGCCGTTTTTGCCCGGCAAATTAATATCCATAATCACTAAATGAATATC  >  1/602526‑603018                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      cTTCAAAATGTTTACGAATTCGACGAATGGTCACATCCACCGTACGATCCTGCGGTTTGAGCTCGCGTCCGGTCATTTTCTTCAATAATTCTTCACGGGTTTGAATTTTGCCCGGATTCTCGCAGAAATGTAACATGGCGCGGAACTCACTACGCGGCAATTTGCGCTCCTCGCCTTCCGGCGTAACCAACGTGCGACTGTTTAAATCCAGTGTCCAGCCGTTAAATTTATATTGTTCCACATGGtgcgtg                                                                                                                                                                                                                                                    >  1:465453/1‑251 (MQ=255)                              gTCACATCCACCGTACGATCCTGCGGTTTGAGCTCGCGTCCGGTCATTTTCTTCAATAATTCTTCACGGGTTTGAATTTTGCCCGGATTCTCGCAGAAATGTAACATGGCGCGGAACTCACTACGCGGCAATTTGCGCTCCTCGCCTTCCGGCGTAACCAACGTGCGACTGTTTAAATCCAGTGTCCAGCCGTTAAATTTATATTGTTCCACATGGTGCGTGCGTTGATTCTCCTGCATGGTAc                                                                                                                                                                                                                              >  1:754216/1‑244 (MQ=255)                              gTCACATCCACCGTACGATCCTGCGGTTTGAGCTCGCGTCCGGTCATTTTCTTCAATAATTCTTCACGGGTTTGAATTTTGCCCGGATTCTCGCAGAAATGTAACATGGCGCGGAACTCACTACGCGGCAATTTGCGCTCCTCGCCTTCCGGCGTAACCAACGTGCGACTGTTTAAATCCAGTGTCCAGCCGTTAAATTTATATTGTTCCACATGGTGCGTGCGTTGATTCTCCTGCATGGTACGCTGCaa                                                                                                                                                                                                                       >  1:389971/1‑251 (MQ=255)                                 acaTCCACCGTACGATCCTGCGGTTTGAGCTCGCGTCCGGTCATTTTCTTCAATAATTCTTCACGGGTTTGAATTTTGCCCGGATTCTCGCAGAAATGTAACATGGCGCGGAACTCACTACGCGGCAATTTGCGCTCCTCGCCTTCCGGCGTAACCAACGTGCGACTGTTTAAATCCAGTGTCCAGCCGTTAAATTTATATTGTTCCACATGGTGCGTGCGTTGATTCTCCTGCATGGTACGCTGCAACAg                                                                                                                                                                                                                    <  1:807788/251‑1 (MQ=255)                                  caTCCACCGTACGATCCTGCGGTTTGAGCTCGCGTCCGGTCATTTTCTTCAATAATTCTTCACGGGTTTGAATTTTGCCCGGATTCTCGCAGAAATGTAACATGGCGCGGAACTCACTACGCGGCAATTTGCGCTCCTCGCCTTCCGGCGTAACCAACGTGCGACTGTTTAAATCCAGTGTCCAGCCGTTAAATTTATATTGTTCCACATGGTGCGTGCGTTGATTCTCCTGCATGGTACGCTGCAACAgg                                                                                                                                                                                                                   >  1:229281/1‑251 (MQ=255)                                  caTCCACCGTACGATCCTGCGGTTTGAGCTCGCGTCCGGTCATTTTCTTCAATAATTCTTCACGGGTTTGAATTTTGCCCGGATTCTCGCAGAAATGTAACATGGCGCGGAACTCACTACGCGGCAATTTGCGCTCCTCGCCTTCCGGCGTAACCAACGTGCGACTGTTTAAATCCAGTGTCCAGCCGTTAAATTTATATTGTTCCACATGGTGCGTGCGTTGATTCTCCTGCATGGTACGCTGCAACAgg                                                                                                                                                                                                                   >  1:566747/1‑251 (MQ=255)                                           tACGATCCTGCGGTTTGAGCTCGCGTCCGGTCATTTTCTTCAATAATTCTTCACGGGTTTGAATTTTGCCCGGATTCTCGCAGAAATGTAACATGGCGCGGAACTCACTACGCGGCAATTTGCGCTCCTCGCCTTCCGGCGTAACCAACGTGCGACTGTTTAAATCCAGTGTCCAGCCGTTAAATTTATATTGTTCCACATGGTGCGTGCGTTGATTCTCCTGCATGGTACGCTGCAACAGGTTTCTGgcg                                                                                                                                                                                                          <  1:836037/251‑1 (MQ=255)                                               aTCCTGCGGTTTGAGCTCGCGTCCGGTCATTTTCTTCAATAATTCTTCACGGGTTTGAATTTTGCCCGGATTCTCGCAGAAATGTAACATGGCGCGGAACTCACTACGCGGCAATTTGCGCTCCTCGCCTTCCGGCGTAACCAACGTGCGACTGTTTAAATCCAGTGTCCAGCCGTTAAATTTATATTGTTCCACATGGTGCGTGCGTTGATTCTCCTGCATGGTACGCTGc                                                                                                                                                                                                                         <  1:374592/232‑1 (MQ=255)                                               aTCCTGCGGTTTGAGCTCGCGTCCGGTCATTTTCTTCAATAATTCTTCACGGGTTTGAATTTTGCCCGGATTCTCGCAGAAATGTAACATGGCGCGGAACTCACTACGCGGCAATTTGCGCTCCTCGCCTTCCGGCGTAACCAACGTGCGACTGTTTAAATCCAGTGTCCAGCCGTTAAATTTATATTGTTCCACATGGTGCGTGCGTTGATTCTCCTGCATGGTACGCTGCAACAGGTTTCTGGCGCGaa                                                                                                                                                                                                      >  1:840169/1‑251 (MQ=255)                                                                      cGGTCATTTTCTTCAATAATTCTTCACGGGTTTGAATTTTGCCCGGATTCTCGCAGAAATGTAACATGGCGCGGAACTCACTACGCGGCAATTTGCGCTCCTCGCCTTCCGGCGTAACCAACGTGCGACTGTTTAAATCCAGTGTCCAGCCGTTAAATTTATATTGTTCCACATGGTGCGTGCGTTGATTCTCCTGCATGGTACGCTGCAACAGGTTTCTGGCGCGAATCGTTAATTCACGCGGGTTGaaa                                                                                                                                                                               <  1:524405/251‑1 (MQ=255)                                                                        gTCATTTTCTTCAATAATTCTTCACGGGTTTGAATTTTGCCCGGATTCTCGCAGAAATGTAACATGGCGCGGAACTCACTACGCGGCAATTTGCGCTCCTCGCCTTCCGGCGTAACCAACGTGCGACTGTTTAAATCCAGTGTCCAGCCGTTAAATTTATATTGTTCCACATGGTGCGTGCGTTGATTCTCCTGCATGGTACGCTGc                                                                                                                                                                                                                         <  1:171931/207‑1 (MQ=255)                                                                         tCATTTTCTTCAATAATTCTTCACGGGTTTGAATTTTGCCCGGATTCTCGCAGAAATGTAACATGGCGCGGAACTCACTACGCGGCAATTTGCGCTCCTCGCCTTCCGGCGTAACCAACGTGCGACTGTTTAAATCCAGTGTCCAGCCGTTAAATTTATATTGTTCCACATGGTGCGTGCGTTGATTCTCCTGCATGGTACGCTGCAACAGGTTTCTGGCGCGAATCGTTAATTCACGCGGGTTGAAAgg                                                                                                                                                                             <  1:812491/250‑1 (MQ=255)                                                                                         ttcttcACGGGTTTGAATTTTGCCCGGATTCTCGCAGAAATGTAACATGGCGCGGAACTCACTACGCGGCAATTTGCGCTCCTCGCCTTCCGGCGTAACCAACGTGCGACTGTTTAAATCCAGTGTCCAGCCGTTAAATTTATATTGTTCCACATGGTGCGTGCGTTGATTCTCCTGCATgg                                                                                                                                                                                                                                 <  1:578369/182‑1 (MQ=255)                                                                                               aCGGGTTTGAATTTTGCCCGGATTCTCGCAGAAATGTAACATGGCGCGGAACTCACTACGCGGCAATTTGCGCTCCTCGCCTTCCGGCGTAACCAACGTGCGACTGTTTAAATCCAGTGTCCAGCCGTTAAATTTATATTGTTCCACATGGTGCGTGCGTTGATTCTCCTGCATGGTACGCTGCAACAGGTTTCTGGCGCGAATCGTTAATTCACGCGGGTTGAAAGGTTTGGTGATGTAATCATCGGCAc                                                                                                                                                      <  1:171811/251‑1 (MQ=255)                                                                                                  ggTTTGAATTTTGCCCGGATTCTCGCAGAAATGTAACATGGCGCGGAACTCACTACGCGGCAATTTGCGCTCCTCGCCTTCCGGCGTAACCAACGTGCGACTGTTTAAATCCAGTGTCCAGCCGTTAAATTTATATTGTTCCACATGGtgcgtgcg                                                                                                                                                                                                                                                  <  1:238955/156‑1 (MQ=255)                                                                                                  ggTTTGAATTTTGCCCGGATTCTCGCAGAAATGTAACATGGCGCGGAACTCACTACGCGGCAATTTGCGCTCCTCGCCTTCCGGCGTAACCAACGTGCGACTGTTTAAATCCAGTGTCCAGCCGTTAAATTTATATTGTTCCACATGGTGCGTGCGTTGATTCTCCTGCATGGTACGCTGCAACAGGTTTCTGGCGCGAATCGTTAATTCACGCGGGTTGAAAGGTTTGGTGATGTAatca                                                                                                                                                             >  1:571416/1‑241 (MQ=255)                                                                                                  ggTTTGAATTTTGCCCGGATTCTCGCAGAAATGTAACATGGCGCGGAACTCACTACGCGGCAATTTGCGCTCCTCGCCTTCCGGCGTAACCAACGTGCGACTGTTTAAATCCAGTGTCCAGCCGTTAAATTTATATTGTTCCACATGGTGCGTGCGTTGATTCTCCTGCATGGTACGCTGCAACAGGTTTCTGGCGCGAATCGTTAATTCACGCGGGTTGAAAGGTTTGGTGATGTAATCATCGGCACCaa                                                                                                                                                   >  1:132999/1‑251 (MQ=255)                                                                                                    tttGAATTTTGCCCGGATTCTCGCAGAAATGTAACATGGCGCGGAACTCACTACGCGGCAATTTGCGCTCCTCGCCTTCCGGCGTAACCAACGTGCGACTGTTTAAATCCAGTGTCCAGCCGTTAAATTTATATTGTTCCACATGGTGCGTGCGTTGATTCTCCTGCATGGTACGCTGCAACAGGTTTCTGGCGCGAATCGTTAATTCACGCGGGTTGAAAGGTTTGGTGATGTAATCATCGGCACCAAtt                                                                                                                                                 <  1:351316/251‑1 (MQ=255)                                                                                                              gCCCGGATTCTCGCAGAAATGTAACATGGCGCGGAACTCACTACGCGGCAATTTGCGCTCCTCGCCTTCCGGCGTAACCAACGTGCGACTGTTTAAATCCAGTGTCCAGCCGTTAAATTTATATTGTTCCACATGGTGCGTGCGTTGATTCTCCTGCATGGTACGCTGCAACAgg                                                                                                                                                                                                                   <  1:827135/175‑1 (MQ=255)                                                                                                                    aTTCTCGCAGAAATGTAACATGGCGCGGAACTCACTACGCGGCAATTTGCGCTCCTCGCCTTCCGGCGTAACCAACGTGCGACTGTTTAAATCCAGTGTCCAGCCGTTAAATTTATATTGTTCCACATGGTGCGTGCGTTGATTCTCCTGCATGGTACGCTGCAACAGGTTTCTGGCGCGAATCGTTAATTCACGCGGGTTGAAAGGTTTGGTGATGTAATCATCGGCACCAATTTCCAAGCCTAGAAtt                                                                                                                                  <  1:522594/250‑1 (MQ=255)                                                                                                                        tcGCAGAAATGTAACATGGCGCGGAACTCACTACGCGGCAATTTGCGCTCCTCGCCTTCCGGCGTAACCAACGTGCGACTGTTTAAATCCAGTGTCCAGCCGTTAAATTTATATTGTTCCACATGGTGCGTGCGTTGATTCTCCTGCATGGTACGCTGCAACAGGTTTCTGGCGCGAATCGTTAATTCACGCGGGTTGAAAGGTTTGGTGATGTAATCATCGGCACCAATTTCCAAGCCTAGAATTTTATc                                                                                                                             <  1:251084/251‑1 (MQ=255)                                                                                                                         cGCAGAAATGTAACATGGCGCGGAACTCACTACGCGGCAATTTGCGCTCCTCGCCTTCCGGCGTAACCAACGTGCGACTGTTTAAATCCAGTGTCCAGCCGTTAAATTTATATTGTTCCACATGGTGCGTGCGTTGATTCTCCTGCATGGTACGCTGCAACAGGTTTCTGGCGCGAATCGTTAATTCACGCGGGTTGAAAGGTTTGGTGATGTAATCATCGGCACCAATTTCCAAGCCTAGAATTTTATcc                                                                                                                            <  1:564488/251‑1 (MQ=255)                                                                                                                           cAGAAATGTAACATGGCGCGGAACTCACTACGCGGCAATTTGCGCTCCTCGCCTTCCGGCGTAACCAACGTGCGACTGTTTAAATCCAGTGTCCAGCCGTTAAATTTATATTGTTCCACATGGTGCGTGCGTTGATTCTCCTGCATGGTACGCTGCAACAGGTTTCTGGCGCGAATCGTTAAtt                                                                                                                                                                                             >  1:620967/1‑184 (MQ=255)                                                                                                                             gAAATGTAACATGGCGCGGAACTCACTACGCGGCAATTTGCGCTCCTCGCCTTCCGGCGTAACCAACGTGCGACTGTTTAAATCCAGTGTCCAGCCGTTAAATTTATATTGTTCCACATGGTGCGTGCGTTGATTCTCCTGCATGGTACGCTGCAACAGGTTTCTGGCGCGAATCGTTAATTCACGCGGGTTGAAAGGTTTGGTGATGTAATCATCGGCACCAATTTCCAAGCCTAGAATTTTATCCACtt                                                                                                                        >  1:78377/1‑251 (MQ=255)                                                                                                                                aTGTAACATGGCGCGGAACTCACTACGCGGCAATTTGCGCTCCTCGCCTTCCGGCGTAACCAACGTGCGACTGTTTAAATCCAGTGTCCAGCCGTTAAATTTATATTGTTCCACATGGTGCGTGCGTTGATTCTCCTGc                                                                                                                                                                                                                                     <  1:772048/139‑1 (MQ=255)                                                                                                                                aTGTAACATGGCGCGGAACTCACTACGCGGCAATTTGCGCTCCTCGCCTTCCGGCGTAACCAACGTGCGACTGTTTAAATCCAGTGTCCAGCCGTTAAATTTATATTGTTCCACATGGTGCGTGCGTTGATTCTCCTGCATGGTACGCTGCAACAGGTTTCTGGCGCGAATCGTTAATTCACGCGGGTTGAAAGGTTTGGTGATGTAATCATCGGCACCAATTTCCAAGCCTAGAAt                                                                                                                                   <  1:30290/237‑1 (MQ=255)                                                                                                                                aTGTAACATGGCGCGGAACTCACTACGCGGCAATTTGCGCTCCTCGCCTTCCGGCGTAACCAACGTGCGACTGTTTAAATCCAGTGTCCAGCCGTTAAATTTATATTGTTCCACATGGTGCGTGCGTTGATTCTCCTGCATGGTACGCTGCAACAGGTTTCTGGCGCGAATCGTTAATTCACGCGGGTTGAAAGGTTTGGTGATGTAATCATCGGCACCAATTTCCAAGCCTAGAATTTTATCCACTTCGt                                                                                                                     >  1:35730/1‑251 (MQ=255)                                                                                                                                     aCATGGCGCGGAACTCACTACGCGGCAATTTGCGCTCCTCGCCTTCCGGCGTAACCAACGTGCGACTGTTTAAATCCAGTGGCCAGCCGTTAAATTTATATTGTTCCACATGGTGCGTGCGTTGATTCTCCTGCATGGTACGCTGCAACAGGTTTCTGGCGCGAATCGTTAATTCACGCGGGTTGAAAGGTTTGGTGATGTAATCATCGGCACCAATTTCCAAGCCTAGAATTTTATCCACTTCGTTAtct                                                                                                                <  1:431181/251‑1 (MQ=255)                                                                                                                                                 aCTCACTACGCGGCAATTTGCGCTCCTCGCCTTCCGGCGTAACCAACGTGCGACTGTTTAAATCCAGTGTCCAGCCGTTAAATTTATATTGTTCCACATGGTGCGTGCGTTGATTCTCCTGCATGGTACGCTGCAACAGGTTTCTGGCGCGAATCGTTAATTCACGCGGGTTGAAAGGTTTGGTGATGTAATCATCGGCACCAATTTCCAAGCCTAGAATTTTATCCACTTCGTTATCTCGACCGGTTaaa                                                                                                    >  1:768849/1‑251 (MQ=255)                                                                                                                                                 aCTCACTACGCGGCAATTTGCGCTCCTCGCCTTCCGGCGTAACCAACGTGCGACTGTTTAAATCCAGTGTCCAGCCGTTAAATTTATATTGTTCCACATGGTGCGTGCGTTGATTCTCCTGCATGGTACGCTGCAACAGGTTTCTGGCGCGAATCGTTAATTCACGCGGGTTGAAAGGTTTGGTGATGTAATCATCGGCACCAATTTCCAAGCCTAGAATTTTATCCACTTCGTTATCTCGACCGGTTaaa                                                                                                    >  1:658796/1‑251 (MQ=255)                                                                                                                                                    cACTACGCGGCAATTTGCGCTCCTCGCCTTCCGGCGTAACCAACGTGCGACTGTTTAAATCCAGTGTCCAGCCGTTAAATTTATATTGTTCCACATGGTGCGTGCGTTGATTCTCCTGCATGGTACGCTGCAACAGGTTTCTGGCGCGAATCGTTAATTCACGCGGGTTGAAAGGTTTGGTGATGTAATCATCGGCACCAATTTCCAAGCCTAGAATTTTATCCACTTCGTTATCTCGACCGGTTaaaaa                                                                                                  >  1:763392/1‑250 (MQ=255)                                                                                                                                                    cACTACGCGGCAATTTGCGCTCCTCGCCTTCCGGCGTAACCAACGTGCGACTGTTTAAATCCAGTGTCCAGCCGTTAAATTTATATTGTTCCACATGGTGCGTGCGTTGATTCTCCTGCATGGTACGCTGCAACAGGTTTCTGGCGCGAATCGTTAATTCACGCGGGTTGAAAGGTTTGGTGATGTAATCATCGGCACCAATTTCCAAGCCTAGAATTTTATCCACTTCGTTATCTCGACCGGTTAAAAAc                                                                                                 <  1:377597/251‑1 (MQ=255)                                                                                                                                                             gCAATTTGCGCTCCTCGCCTTCCGGCGTAACCAACGTGCGACTGTTTAAATCCAGTGTCCAGCCGTTAAATTTATATTGTTCCACATGGTGCGTGCGTTGATTCTCCTGCATGGTACGCTGCAACAGGTTTCTGGCGCGAATCGTTAATTCACGCGGGTTGAAAGGTTTGGTGATGTAATCATCGGCACCAATTTCCAAGCCTAGAATTTTATCCACTTCGTTATCTCGACCGGTTAAAAACATCAGTGc                                                                                         <  1:314727/250‑1 (MQ=255)                                                                                                                                                             gCAATTTGCGCTCCTCGCCTTCCGGCGTAACCAACGTGCGACTGTTTAAATCCAGTGTCCAGCCGTTAAATTTATATTGTTCCACATGGTGCGTGCGTTGATTCTCCTGCATGGTACGCTGCAACAGGTTTCTGGCGCGAATCGTTAATTCACGCGGGTTGAAAGGTTTGGTGATGTAATCATCGGCACCAATTTCCAAGCCTAGAATTTTATCCACTTCGTTATCTCGACCGGTTAAAAACATCAGTGc                                                                                         <  1:898477/250‑1 (MQ=255)                                                                                                                                                              cAATTTGCGCTCCTCGCCTTCCGGCGTAACCAACGTGCGACTGTTTAAATCCAGTGTCCAGCCGTTAAATTTATATTGTTCCACATGGTGCGTGCGTTGATTCTCCTGCATGGTACGCTGCAACAGGTTTCTGGCGCGAATCGTTAATTCACGCGGGTTGAAAGGTTTGGTGATGTAATCAACGGCACCAATTTCCAAGCCTAGAATTTTATCCACTTCGTTATCTCGACCGGTTAAAAACATCAGTGCaa                                                                                       <  1:174235/251‑1 (MQ=255)                                                                                                                                                                 tttGCGCTCCTCGCCTTCCGGCGTAACCAACGTGCGACTGTTTAAATCCAGTGTCCAGCCGTTAAATTTATATTGTTCCACATGGTGCGTGCGTTGATTCTCCTGCATGGTACGCTGCAACAGGTTTCTGGCGCGAATCGTTAATTCACGCGGGTTGAAAGGTTTGGTGATGTAATCATCGGCACCAATTTCCAAGCCTAGAATTTTATCCACTTCGTTAtct                                                                                                                >  1:639540/1‑223 (MQ=255)                                                                                                                                                                 tttGCGCTCCCCGCCTTCCGGCGTAACCAACGTGCGACTGTTTAAATCCAGTGTCCAGCCGTTAAATTTATATTGTTCCACATGGTGCGTGCGTTGATTCTCCTGCATGGTACGCTGCAACAGGTTTCTGGCGCGAATCGTTAATTCACGCGGGTTGAAAGGTTTGGTGATGTAATCATCGGCACCAATTTCCAAGCCTAGAATTTTATCCACTTCGTTATCTCGACCGGTTAAAAACATCAGTGCAATGt                                                                                    <  1:85820/251‑1 (MQ=255)                                                                                                                                                                       ctcctcGCCTTCCGGCGTAACCAACGTGCGACTGTTTAAATCCAGTGTCCAGCCGTTAAATTTATATTGTTCCACATGGTGCGTGCGTTGATTCTCCTGCATGGTACGCTGCAACAGGTTTCTGGCGCGAATCGTTAATTCACGCGGGTTGAAAGGTTTGGTGATGTAATCATCGGCACCAATTTCCAAGCCTAGAATTTTATCCACTTCGTTATCTCGACCGGTTAAAAACATCAGTGCAATGTCCGCAt                                                                              >  1:85967/1‑251 (MQ=255)                                                                                                                                                                         cctcGCCTTCCGGCGTAACCAACGTGCGACTGTTTAAATCCAGTGTCCAGCCGTTAAATTTATATTGTTCCACATGGTGCGTGCGTTGATTCTCCTGCATGGTACGCTGCAACAGGTTTCTGGCGCGAATCGTTAATTCACGCGGGTTGAAAGGTTTGGTGATGTAATCATCGGCACCAATTTCCAAGCCTAGAATTTTATCCACTTCGTTATCTCGACCGGTTAAAAACATCAGTGCAATGTCCGCAttt                                                                            >  1:760672/1‑251 (MQ=255)                                                                                                                                                                                        tAACCAACGTGCGACTGTTTAAATCCAGTGTCCAGCCGTTAAATTTATATTGTTCCACATGGTGCGTGCGTTGATTCTCCTGCATGGTACGCTGCAACAGGTTTCTGGCGCGAATCGTTAATTCACGCGGGTTGAAAGGTTTGGTGATGTAATCATCGGCACCAATTTCCAAGCCTAGAATTTTATCCACTTCGTTATCTCGACCGGTTAAAAACATCAGTGCAATGTCCGCATTTTCACGCAATTCACg                                                              <  1:640006/250‑1 (MQ=255)                                                                                                                                                                                         aaCCAACGTGCGACTGTTTAAATCCAGTGTCCAGCCGTTAAATTTATATTGTTCCACATGGTGCGTGCGTTGATTCTCCTGCATGGTACGCTGCAACAGGTTTCTGGCGCGAATCGTTAATTCACGCGGGTTGAAAGGTTTGGTGATGTAATCATCGGCACCAATTTCCAAGCCTAGAATTTTATCCACTTCGTTATCTCGACCGGTTAAAAACATCAGTGCAATGTCCGCATTTTCACGCAATTCACggg                                                            <  1:329910/251‑1 (MQ=255)                                                                                                                                                                                              aCGTGCGACTGTTTAAATCCAGTGTCCAGCCGTTAAATTTATATTGTTCCACATGGTGCGTGCGTTGATTCTCCTGCATGGTACGCTGCAACAGGTTTCTGGCGCGAATCGTTAATTCACGCGGGTTGAAAg                                                                                                                                                                              <  1:344006/132‑1 (MQ=255)                                                                                                                                                                                                        gTTTAAATCCAGTGTCCAGCCGTTAAATTTATATTGTTCCACATGGTGCGTGCGTTGATTCTCCTGCATGGTACGCTGCAACAgg                                                                                                                                                                                                                   <  1:161123/85‑1 (MQ=255)                                                                                                                                                                                                        gTTTAAATCCAGTGTCCAGCCGTTAAATTTATATTGTTCCACATGGTGCGTGCGTTGATTCTCCTGCATGGTACGCTGCAACAGGTTTCTGGCGCGAATCGTTAATTCAcgcg                                                                                                                                                                                       <  1:83628/113‑1 (MQ=255)                                                                                                                                                                                                        gTTTAAATCCAGTGTCCAGCCGTTAAATTTATATTGTTCCACATGGTGCGTGCGTTGATTCTCCTGCATGGTACGCTGCAACAGGTTTCTGGCGCGAATCGTTAATTCACGCGGGTTGAAAGGTTTGGTGATGTAATCATCGGCACCAATTTCCAAGCCTAGAATTTTATCCACTTCGTTAt                                                                                                                  <  1:221840/182‑1 (MQ=255)                                                                                                                                                                                                        gTTTAAATCCAGTGTCCAGCCGTTAAATTTATATTGTTCCACATGGTGCGTGCGTTGATTCTCCTGCATGGTACGCTGCAACAGGTTTCTGGCGCGAATCGTTAATTCACGCGGGTTGAAAGGTTTGGTGATGTAATCATCGGCACCAATTTCCAAGCCTAGAATTTTATCCACTTCGTTATCTCGACCGGTTAAAAACATCAGTGCAATGTCCGCATTTTCACGCAATTCACGGGCAAGCAt                                                     <  1:498426/243‑1 (MQ=255)                                                                                                                                                                                                        gTTTAAATCCAGTGTCCAGCCGTTAAATTTATATTGTTCCACATGGTGCGTGCGTTGATTCTCCTGCATGGTACGCTGCAACAGGTTTCTGGCGCGAATCGTTAATTCACGCGGGTTGAAAGGTTTGGTGATGTAATCATCGGCACCAATTTCCAAGCCTAGAATTTTATCCACTTCGTTATCTCGACCGGTTAAAAACATCAGTGCAATGTCCGCATTTTCACGCAATTCACGGGCAAGCATTAAGCCGt                                             >  1:488177/1‑251 (MQ=255)                                                                                                                                                                                                        gTTTAAATCCAGTGTCCAGCCGTTAAATTTATATTGTTCCACATGGTGCGTGCGTTGATTCTCCTGCATGGTACGCTGCAACAGGTTTCTGGCGCGAATCGTTAATTCACGCGGGTTGAAAGGTTTGGTGATGTAATCATCGGCACCAATTTCCAAGCCTAGAATTTTATCCACTTCGTTATCTCGACCGGTTAAAAACATCAGTGCAATGTCCGCATTTTCACGCAATTCACGGGCAAGCATTAAGCCGt                                             >  1:174890/1‑251 (MQ=255)                                                                                                                                                                                                        gTTTAAATCCAGTGTCCAGCCGTTAAATTTATATTGTTCCACATGGTGCGTGCGTTGATTCTCCTGCATGGTACGCTGCAACAGGTTTCTGGCGCGAATCGTTAATTCACGCGGGTTGAAAGGTTTGGTGATGTAATCATCGGCACCAATTTCCAAGCCTAGAATTTTATCCACTTCGTTATCTCGACCGGTTAAAAACATCAGTGCAATGTCCGCATTTTCACGCAATTCACGGGCAAGCATTAAGCCGt                                             >  1:268072/1‑251 (MQ=255)                                                                                                                                                                                                             aaTCCAGTGTCCAGCCGTTAAATTTATATTGTTCCACATGGTGCGTGCGTTGATTCTCCTGCATGGTACGCTGCAACAGGTTTCTGGCGCGAATCGTTAATTCACGCGGGTTGAAAg                                                                                                                                                                              >  1:322571/1‑117 (MQ=255)                                                                                                                                                                                                                   gtgtCCAGCCGTTAAATTTATATTGTTCCACATGGTGCGTGCGTTGATTCTCCTGCATGGTACGCTGCAACAGGTTTCTGGCGCGAATCGTTAATTCACGCGGGTTGAAAGGTTTGGTGATGTAATCATCGGCACCAATTTCCAAGCCTAGAATTTTATCCACTTCGTTATCTCGACCGGTTAAAAACATCAGTGCAATGTCCGCATTTTCACGCAATTCACGGGc                                                           >  1:248873/1‑226 (MQ=255)                                                                                                                                                                                                                     gtCCAGCCGTTAAATTTATATTGTTCCACATGGTGCGTGCGTTGATTCTCCTGCATGGTACGCTGCAACAGGTTTCTGGCGCGAATCGTTAATTCACGCGGGTTGAAAGGTTTGGTGATGTAATCATCGGCACCAATTTCCAAGCCTAGAATTTTATcc                                                                                                                            <  1:13993/159‑1 (MQ=255)                                                                                                                                                                                                                                 aaTTTATATTGTTCCACATGGTGCGTGCGTTGATTCTCCTGCATGGTACGCTGCAACAGGTTTCTGGCGCGAATCGTTAATTCACGCGGGTTGAAAGGTTTGGTGATGTAATCATCGGCACCAATTTCCAAGCCTAGAATTTTATCCACTTCGTTATCTCGACCGGTTAAAAACATCAGTGCAATGTCCGCATTTTCACGCAATTCACGGGCAAGCATTAAGCCGTTTTTGCCCGGCAAATTAATATCCAt                    >  1:901738/1‑251 (MQ=255)                                                                                                                                                                                                                                    ttATATTGTTCCACATGGTGCGTGCGTTGATTCTCCTGCATGGTACGCTGCAACAGGTTTCTGGCGCGAATCGTTAATTCACGCGGGTTGAAAGGTTTGGTGATGTAATCATCGGCACCAATTTCCAAGCCTAGAATTTTATCCACTTCGTTATCTCGACCGGTTAAAAACATCAGTGCAATGTCCGCATTTTCACGCAATTCACGGGCAAGCATTAAGCCGTTTTTGCCCGGCAAATTAATATCCATAAt                 <  1:325024/251‑1 (MQ=255)                                                                                                                                                                                                                                       tatTGTTCCACATGGTGCGTGCGTTGATTCTCCTGCATGGTACGCTGCAACAGGTTTCTGGCGCGAATCGTTAATTCACGCGAGTTGAAAGGTTTGGTGATGTAATCATCGGCACCAATTTCCAAGCCTAGAATTTTATCCACTTCGTTATCTCGACCGGTTAAAAACATCAGTGCAATGTCCGCATTTTCACGCAATTCACGGGCAAGCATTAAGCCGTTTTTGCCCGGCAAATTAATATCCATAATCAc              <  1:868770/251‑1 (MQ=255)                                                                                                                                                                                                                                        atTGTTCCACATGGTGCGTGCGTTGATTCTCCTGCATGGTACGCTGCAACAGGTTTCTGGCGCGAATCGTTAATTCACGCGGGTTGAAAGGTTTGGTGATGTAATCATCGGCACCAATTTCCAAGCCTAGAATTTTATCCACTTCGTTATCTCGACCGGTTAAAAACATCAGTGCAATGTCCGCATTTTCACGCAATTCACGGGCAAGCATTAAGCCGTTTTTGCCCGGCAAATTAATATCCATAATCACt             <  1:329828/251‑1 (MQ=255)                                                                                                                                                                                                                                         ttGTTCCACATGGTGCGTGCGTTGATTCTCCTGCATGGTACGCTGCAACAGGTTTCTGGCGCGAATCGTTAATTCACGCGGGTTGAAAGGTTTGGTGATGTAATCATCGGCACCAATTTCCAAGCCTAGAATTTTATCCACTTCGTTATCTCGACCGGTTAAAAACATc                                                                                              >  1:51642/1‑169 (MQ=255)                                                                                                                                                                                                                                           gTTCCACATGGTGCGTGCGTTGATTCTCCTGCATGGTACGCTGCAACAGGTTTCTGGCGCGAATCGTTAATTCACGCGGGTTGAAAGGTTTGGTGATGTAATCATCGGCACCAATTTCCAAGCCTAGAATTTTATCCACTTCGTTATCTCGACCGGTTaaaaa                                                                                                  >  1:71191/1‑163 (MQ=255)                                                                                                                                                                                                                                           gTTCCACATGGTGCGTGCGTTGATTCTCCTGCATGGTACGCTGCAACAGGTTTCTGGCGCGAATCGTTAATTCACGCGGGTTGAAAGGTTTGGTGATGTAATCATCGGCACCAATTTCCAAGCCTAGAATTTTATCCACTTCGTTATCTCGACCGGTTAAAAACATCAGTGc                                                                                         >  1:505761/1‑172 (MQ=255)                                                                                                                                                                                                                                           gTTCCACATGGTGCGTGCGTTGATTCTCCTGCATGGTACGCTGCAACAGGTTTCTGGCGCGAATCGTTAATTCACGCGGGTTGAAAGGTTTGGTGATGTAATCATCGGCACCAATTTCCAAGCCTAGAATTTTATCCACTTCGTTATCTCGACCGGTTAAAAACATCAGTGCAATGTCCGCAtttt                                                                           >  1:892245/1‑186 (MQ=255)                                                                                                                                                                                                                                           gTTCCACATGGTGCGTGCGTTGATTCTCCTGCATGGTACGCTGCAACAGGTTTCTGGCGCGAATCGTTAATTCACGCGGGTTGAAAGGTTTGGTGATGTAATCATCGGCACCAATTTCCAAGCCTAGAATTTTATCCACTTCGTTATCTCGACCGGTTAAAAACATCAGTGCAATGTCCGCATTTTCACGCAATTCACGGGCAAGCAt                                                     >  1:38216/1‑208 (MQ=255)                                                                                                                                                                                                                                           gTTCCACATGGTGCGTGCGTTGATTCTCCTGCATGGTACGCTGCAACAGGTTTCTGGCGCGAATCGTTAATTCACGCGGGTTGAAAGGTTTGGTGATGTAATCATCGGCACCAATTTCCAAGCCTAGAATTTTATCCACTTCGTTATCTCGACCGGTTAAAAACATCAGTGCAATGTCCGCATTTTCACGCAATTCACGGGCAAGCAt                                                     >  1:683956/1‑208 (MQ=255)                                                                                                                                                                                                                                           gTTCCACATGGTGCGTGCGTTGATTCTCCTGCATGGTACGCTGCAACAGGTTTCTGGCGCGAATCGTTAATTCACGCGGGTTGAAAGGTTTGGTGATGTAATCATCGGCACCAATTTCCAAGCCTAGAATTTTATCCACTTCGTTATCTCGACCGGTTAAAAACATCAGTGCAATGTCCGCATTTTCACGCAATTCACGGGCAAGCATTAAGCCGTTTTTGCCCGGCAAATTaa                           <  1:74781/234‑1 (MQ=255)                                                                                                                                                                                                                                           gTTCCACATGGTGCGTGCGTTGATTCTCCTGCATGGTACGCTGCAACAGGTTTCTGGCGCGAATCGTTAATTCACGCGGGTTGAAAGGTTTGGTGATGTAATCATCGGCACCAATTTCCAAGCCTAGAATTTTATCCACTTCGTTATCTCGACCGGTTAAAAACATCAGTGCAATGTCCGCATTTTCACGCAATTCACGGGCAAGCATTAAGCCGTTTTTGCCCGGCAAATTAatat                        <  1:897637/237‑1 (MQ=255)                                                                                                                                                                                                                                           gTTCCACATGGTGCGTGCGTTGATTCTCCTGCATGGTACGCTGCAACAGGTTTCTGGCGCGAATCGTTAATTCACGCGGGTTGAAAGGTTTGGTGATGTAATCATCGGCACCAATTTCCAAGCCTAGAATTTTATCCACTTCGTTATCTCGACCGGTTAAAAACATCAGTGCAATGTCCGCATTTTCACGCAATTCACGGGCAAGCATTAAGCCGTTTTTGCCCGGCAAATTAatat                        <  1:480036/237‑1 (MQ=255)                                                                                                                                                                                                                                           gTTCCACATGGTGCGTGCGTTGATTCTCCTGCATGGTACGCTGCAACAGGTTTCTGGCGCGAATCGTTAATTCACGCGGGTTGAAAGGTTTGGTGATGTAATCATCGGCACCAATTTCCAAGCCTAGAATTTTATCCACTTCGTTATCTCGACCGGTTAAAAACATCAGTGCAATGTCCGCATTTTCACGCAATTCACGGGCAAGCATTAAGCCGTTTTTGCCCGGCAAATTAATATCCATAATCACTaaa          >  1:433723/1‑251 (MQ=255)                                                                                                                                                                                                                                           gTTCCACATGGTGCGTGCGTTGATTCTCCTGCATGGTACGCTGCAACAGGTTTCTGGCGCGAATCGTTAATTCACGCGGGTTGAAAGGTTTGGTGATGTAATCATCGGCACCAATTTCCAAGCCTAGAATTTTATCCACTTCGTTATCTCGACCGGTTAAAAACATCAGTGCAATGTCCGCATTTTCACGCAATTCACGGGCAAGCATTAAGCCGTTTTTGCCCGGCAAATTAATATCCATAATCACTaaa          >  1:709386/1‑251 (MQ=255)                                                                                                                                                                                                                                           gTTCCACATGGTGCGTGCGTTGATTCTCCTGCATGGTACGCTGCAACAGGTTTCTGGCGCGAATCGTTAATTCACGCGGGTTGAAAGGTTTGGTGATGTAATCATCGGCACCAATTTCCAAGCCTAGAATTTTATCCACTTCGTTATCTCGACCGGTTAAAAACATCAGTGCAATGTCCGCATTTTCACGCAATTCACGGGCAAGCATTAAGCCGTTTTTGCCCGGCAAATTAATATCCATAATCACTaaa          >  1:306954/1‑251 (MQ=255)                                                                                                                                                                                                                                             tCCACATGGTGCGTGCGTTGATTCTCCTGCATGGTACGCTGCAACAGGTTTCTGGCGCGAATCGTTAATTCACGCGGGTTGAAAGGTTTGGTGATGTAATCATCGGCACCAATTTCCAAGCCTAGAATTTTATCCACTTCGTTATCTCGACCGGTTAAAAACATCAGTGc                                                                                         <  1:747374/170‑1 (MQ=255)                                                                                                                                                                                                                                             tCCACATGGTGCGTGCGTTGATTCTCCTGCATGGTACGCTGCAACAGGTTTCTGGCGCGAATCGTTAATTCACGCGGGTTGAAAGGTTTGGTGATGTAATCATCGGCACCAATTTCCAAGCCTAGAATTTTATCCACTTCGTTATCTCGACCGGTTAAAAACATCAGTGCAATGTc                                                                                   >  1:452924/1‑176 (MQ=255)                                                                                                                                                                                                                                             tCCACATGGTGCGTGCGTTGATTCTCCTGCATGGTACGCTGCAACAGGTTTCTGGCGCGAATCGTTAATTCACGCGGGTTGAAAGGTTTGGTGATGTAATCATCGGCACCAATTTCCAAGCCTAGAATTTTATCCACTTCGTTATCTCGACCGGTTAAAAACATCAGTGCAATGTCCGCATTTTCACGCAATTCACGGGCAAGCATTAAGCCGTTTTTGCCCGGCAAATTAATATCCATAATc                <  1:469432/243‑1 (MQ=255)                                                                                                                                                                                                                                             tCCACATGGTGCGTGCGTTGATTCTCCTGCATGGTACGCTGCAACAGGTTTCTGGCGCGAATCGTTAATTCACGCGGGTTGAAAGGTTTGGTGATGTAATCATCGGCACCAATTTCCAAGCCTAGAATTTTATCCACTTCGTTATCTCGACCGGTTAAAAACATCAGTGCAATGTCCGCATTTTCACGCAATTCACGGGCAAGCATTAAGCCGTTTTTGCCCGGCAAATTAATATCCATAATCACTAAATg        >  1:819775/1‑251 (MQ=255)                                                                                                                                                                                                                                             tCCACATGGTGCGTGCGTTGATTCTCCTGCATGGTACGCTGCAACAGGTTTCTGGCGCGAATCGTTAATTCACGCGGGTTGAAAGGTTTGGTGATGTAATCATCGGCACCAATTTCCAAGCCTAGAATTTTATCCACTTCGTTATCTCGACCGGTTAAAAACATCAGTGCAATGTCCGCATTTTCACGCAATTCACGGGCAAGCATTAAGCCGTTTTTGCCCGGCAAATTAATATCCATAATCACTAAATg        >  1:316145/1‑251 (MQ=255)                                                                                                                                                                                                                                             tCCACATGGTGCGTGCGTTGATTCTCCTGCATGGTACGCTGCAACAGGTTTCTGGCGCGAATCGTTAATTCACGCGGGTTGAAAGGTTTGGTGATGTAATCATCGGCACCAATTTCCAAGCCTAGAATTTTATCCACTTCGTTATCTCGACCGGTTAAAAACATCAGTGCAATGTCCGCATTTTCACGCAATTCACGGGCAAGCATTAAGCCGTTTTTGCCCGGCAAATTAATATCCATAATCACTAAATg        <  1:708979/251‑1 (MQ=255)                                                                                                                                                                                                                                             tCCACATGGTGCGTGCGTTGATTCTCCTGCATGGTACGCTGCAACAGGTTTCTGGCGCGAATCGTTAATTCACGCGGGTTGAAAGGTTTGGTGATGTAATCATCGGCACCAATTTCCAAGCCTAGAATTTTATCCACTTCGTTATCTCGACCGGTTAAAAACATCAGTGCAATGTCCGCATTTTCACGCAATTCACGGGCAAGCATTAAGCCGTTTTTGCCCGGCAAATTAATATCCATAATCACTAAATg        >  1:525314/1‑251 (MQ=255)                                                                                                                                                                                                                                             tCCACATGGTGCGTGCGTTGATTCTCCTGCATGGTACGCTGCAACAGGTTTCTGGCGCGAATCGTTAATTCACGCGGGTTGAAAGGTTTGGTGATGTAATCATCGGCACCAATTTCCAAGCCTAGAATTTTATCCACTTCGTTATCTCGACCGGTTAAAAACATCAGTGCAATGTCCGCATTTTCACGCAATTCACGGGCAAGCATTAAGCCGTTTTTGCCCGGCAAATTAATATCCATAATCACTAAATg        >  1:558966/1‑251 (MQ=255)                                                                                                                                                                                                                                                   tGGTGCGTGCGTTGATTCTCCTGCATGGTACGCTGCAACAGGTTTCTGGCGCGAATCGTTAATTCACGCGGGTTGAAAGGTTTGGTGATGTAATCATCGGCACCAATTTCCAAGCCTAGAATTTTATCCACTTCGTTATCTCGACCGGTTAAAAACATCAGTGCAATGTCCGCATTTTCACGCAATTCACGGGCAAGCATTAAGCCGTTTTTGCCCGGCAAATTAATATCCATAATCACTAAATGAATATc  <  1:714894/251‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      CTTCAAAATGTTTACGAATTCGACGAATGGTCACATCCACCGTACGATCCTGCGGTTTGAGCTCGCGTCCGGTCATTTTCTTCAATAATTCTTCACGGGTTTGAATTTTGCCCGGATTCTCGCAGAAATGTAACATGGCGCGGAACTCACTACGCGGCAATTTGCGCTCCTCGCCTTCCGGCGTAACCAACGTGCGACTGTTTAAATCCAGTGTCCAGCCGTTAAATTTATATTGTTCCACATGGTGTGTGCGTTGATTCTCCTGCATGGTACGCTGCAACAGGTTTCTGGCGCGAATCGTTAATTCACGCGGGTTGAAAGGTTTGGTGATGTAATCATCGGCACCAATTTCCAAGCCTAGAATTTTATCCACTTCGTTATCTCGACCGGTTAAAAACATCAGTGCAATGTCCGCATTTTCACGCAATTCACGGGCAAGCATTAAGCCGTTTTTGCCCGGCAAATTAATATCCATAATCACTAAATGAATATC  >  1/602526‑603018

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑