Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	572,518	T→C	T69A (ACG→GCG)	unknown ←	Probable transcriptional regulatory protein YebC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	572,518	0	T	C	100.0%	226.0 / NA	66	T69A (ACG→GCG)	unknown	Probable transcriptional regulatory protein YebC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/43);  total (23/43)

TCAAAGCGTCTTCATCCGCCTGATTCACTAAAATCAAGCCTTTTTTACTAAACAAATAGCCCACGGAACCCTCGGTGCCTAAGTTACCGCCACATTTGGTAAAACTCGGGCGCACTTGGGAAATAGTGCGATTGGCGTTATCGCTTAAACATTCCACCATCACTGCCGCACCGCCCGGACCGTAACCTTCATACACGCGGGTTTCCATGTTGGTATCATCGCCACCGCCGACGCCGCGCTCAATCGTACGGTTAATGGTATCACGGGTCATATTATTGCTTAATGCTTTATCTACCGCCGCACGCAGACGCGGGTTAGAACTAACATCACCGCCACCGATTTTAGCGGCAGTCACCAATTCACGAATTAATTTGGTAAAAATTTTCCCGCGCTGAGCATCTTGTGCCGCTTTACGGTGTTTAATATTTGCCCACTTACTATGGCCTGCCATTTTGTATTCCTTCTCAATTTATTTAAATTCGGCTTATTTC  >  1/572272‑572762                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     tCAAGGCGTCTTCATCCGCCGGATTCACTAAAATCAAGCCTTTTTTACTAAACAAATAGCCCACGGAACCCTCGGTGCCTAAGTTACCGCCACATTTGGTAAAACTCGGGCGCACTTGGGAAATAGTGCGATTGGCGTTATCGCTTAAACATTCCACCATCACTGCCGCACCGCCCGGACCGTAACCTTCATACACGCGGGTTTCCATGTTGGTATCATCGCCACCGCCGACGCCGCGCTCAATCGCACgg                                                                                                                                                                                                                                                  <  1:854193/251‑1 (MQ=255)        gTCTTCATCCGCCTGATTCACTAAAATCAAGCCTTTTTTACTAAACAAATAGCCCACGGAACCCTCGGTGCCTAAGTTACCGCCACATTTGGTAAAACTCGGGCGCACTTGGGAAATAGTGCGATTGGCGTTATCGCTTAAACATTCCACCATCACTGCCGCACCGCCCGGACCGTAACCTTCATACACGCGGGTTTCCATGTTGGTATCATCGCCACCGCCGACGCCGCGCTCAATCGCACGGTTAATgg                                                                                                                                                                                                                                           >  1:730446/1‑251 (MQ=255)        gTCTTCACCCGCCTGATTCCCTAAAATCAAGCCTTTTTTACTAAACAAATACCCCCCGGAACCCTCGGTGCCTATGTTCCCGCCACATTTGGTAAAACTCGGGCGCACTTGGGAAATAGTGCGATTGGCGTTATCGCTTAAACATTCCACCATCACTGCCGCACCGCCCGGACCGTAACCTTCATACACGCGGGTTTCCATGTTGGTATCATCGCCACCGCCGACGCCGCGCTCAATCGCACGGTTAATg                                                                                                                                                                                                                                            <  1:31842/250‑1 (MQ=255)         tCTTCATCCGCCTGATTCACTAAAATCAAGCCTTTTTTACTAAACAAATAGCCCCCGGAACCCTCGGTGCCTAAGTTACCGCCACATTTGGTAAAACTCGGGCGCACTTGGGAAATAGTGCGATTGGCGTTATCGCTTAAACATTCCACCATCACTGCCGCACCGCCCGGACCGTAACCTTCATACACGCGGGTTTCCATGTTGGTATCATCGCCACCGCCGACGCCGCGCTCAATCGCACGGTTAATGGt                                                                                                                                                                                                                                          <  1:693870/251‑1 (MQ=255)         tCTTCATCCGCCTGATTCACTAAAATCAAGCCTTTTTTACTAAACAAATAGCCCACGGACCCCTCGGTGCCTAAGTTCCCGCCACATTTGGTAAAACTCGGGCGCACTTGGGAAATAGTGCGATTGGCGTTATCGCTTAAACATTCCACCATCACTGCCGCACCGCCCGGACCGTAACCTTCATACACGCGGGTTTCCATGTTGGTATCATCGCCACCGCCGACGCCGCGCTCAATCGCACGGTTAATGGt                                                                                                                                                                                                                                          <  1:209735/251‑1 (MQ=255)         tCTTCATCCGCCTGATTCACTAAAATCAAGCCTTTTTTACTAAACAAATAGCCCACGGAACCCTCGGTGCCTAAGTTACCGCCACATTTGGTAAAACTCGGGCGCACTTGGGAAATAGTGCGATTGGCGTTATCGCTTAAACATTCCACCATCACTGCCGCACCGCCCGGACCGTAACCTTCATACACGCGGGTTTCCATGTTGGTATCATCGCCACCGCCGACGCCGCGCTCAATCGCACGGTTAATgg                                                                                                                                                                                                                                           <  1:286536/250‑1 (MQ=255)         tCTTCATCCGCCTGATTCACTAAAATCAAGCCTTTTTTACTAAACAAATAGCCCACGGAACCCTCGGTGCCTAAGTTACCGCCACATTTGGTAAAACTCGGGCGCACTTGGGAAATAGTGCGATTGGCGTTATCGCTTAAACATTCCACCATCACTGCCGCACCGCCCGGACCGTAACCTTCATACACGCGGGTTTCCATGTTGGTATCATCGCCACCGCCGACGCCGCGCTCAATCGCACGGTTAATGGt                                                                                                                                                                                                                                          <  1:173191/251‑1 (MQ=255)         tCTTCATCCGCCTGATTCACAAAAATCAAGCTTTTTTTACTAACCAAATAGCCCCGGGAACCCTCGGTGCCTAAGTTACCGCCACATTTGGTAAAACTCGGGCGCACTTGGGAAATAGTGCGATTGGCGTTATCGCTTAAACATTCCACCATCACTGCCGCACCGCCCGGACCGTAACCTTCATACACGCGGGTTTCCATGTTGGTATCATCGCCACCGCCGACGCCGCGCTCAATCGCACGGTTAATgg                                                                                                                                                                                                                                           <  1:721524/250‑1 (MQ=255)         tCTTCACCCGCCTGTTTCACTAAAATCAAGCCTTTTTTACTAAACAAATAGCCCACGGAACCCTCGGTGCCTAAGTTACCGCCACATTTGGTAAAACTCGGGCGCACTTGGGAAATAGTGCGATTGGCGTTATCGCTTAAACATTCCACCATCACTGCCGCACCGCCCGGACCGTAACCTTCATACACGCGGGTTTCCATGTTGGTATCATCGCCACCGCCGACGCCGCGCTCAATCGCACGGTTAATGGt                                                                                                                                                                                                                                          <  1:41763/251‑1 (MQ=255)           ttCACCCGCCTGATTCACTAAAATCAAGCCTTTTTTACTAAACAAATAGCCCACGGACCCCTCGGTCCCTAAGTTCCCGCCACATTTGGTAAAACTCGGGCGCACTTGGGAAATAGTGCGATTGGCGTTATCGCTTAAACATTCCACCATCACTGCCGCACCGCCCGGACCGTAACCTTCATACACGCGGGTTTCCATGTTGGTATCATCGCCACCGCCGACGCCGCGCTCAATCGCACGGTTAATGGTAt                                                                                                                                                                                                                                        <  1:575298/251‑1 (MQ=255)            tCATCCGCCTGATTCACTAAAATCAAGCCTTTTTTACTAAACAAATAGCCCACGGAACCCTCGGTGCCTAAGTTACCGCCACATTTGGTAAAACTCGGGCGCACTTGGGAAATAGTGCGATTGGCGTTATCGCTTAAACATTCCACCATCACTGCCGCACCGCCCGGACCGTAACCTTCATACACGCGGGTTTCCATGTTGGTATCATCGCCACCGCCGACGCCGCGCTCAATCGCACGGTTAATGGTAt                                                                                                                                                                                                                                        >  1:93443/1‑250 (MQ=255)            tCATCCGCCTGATTCACTAAAATCAAGCCTTTTTTACTAAACAAATAGCCCACGGAACCCTCGGTGCCTAAGTTA‑CGCCACATTTGGTAAAACTCGGGCGCACTTGGGAAATAGTGCGATTGGCGTTATCGCTTAAACATTCCACCATCACTGCCGCACCGCCCGGACCGTAACCTTCATACACGCGGGTTTCCATGTTGGTATCATCGCCACCGCCGACGCCGCGCTCAATCGCACGGTTAATGGt                                                                                                                                                                                                                                          >  1:697652/1‑247 (MQ=255)                ccGCCTGATTCACTAAAATCAAGCCTTTTTTACTAAACAAATAGCCCACGGAACCCTCGGTGCCTAAGTTACCGCCACATTTGGTAAAACTCGGGCGCACTTGGGAAATAGTGCGATTGGCGTTATCGCTTAAACATTCCACCATCACTGCCGCACCGCCCGGACCGTAACCTTCATACACGCGGGTTTCCATGTTGGTATCATCGCCACCGCCGACGCCGCGCTCAATCGCACGGTTAATGGTATCACgg                                                                                                                                                                                                                                   <  1:741247/251‑1 (MQ=255)                  cccTGATTCACTAAAATCAAGCCTTTTTTACAAAACAAATAGCCCACGGACCCCTCGGTGCCTAAGTTACCGCCACATTTGGTAAAACTCGGGCGCACTTGGGAAATAGTGCGATTGGCGTTATCGCTTAAACATTCCACCATCACTGCCGCACCGCCCGGACCGTAACCTTCATACACGCGGGTTTCCATGTTGGTATCATCGCCACCGCCGACGCCGCGCTCAATCGCACGGTTAATGGTATCACGGGt                                                                                                                                                                                                                                 <  1:542907/250‑1 (MQ=255)                          cACTAAAATCAAGCCTTTTTTACTAAAAAAATAGCCCACGGAACCCTCGGTGCCTAAGTTACCGCCACATTTGGTAAAACTCGGGCGCACTTGGGAAATAGTGCGATTGGCGTTATCGCTTAAACATTCCACCATCACTGCCGCACCGCCCGGACCGTAACCTTCATACACGCGGGTTTCCATGTTGGTATCATCGCCACCGCCGACGCCGCGCTCAATCGCACGGTTAATGGTATCACGGGTCAtattat                                                                                                                                                                                                                         <  1:769634/251‑1 (MQ=255)                            cTAAAATCAACCCTTTTTTACTAAACAAATAGCCCACGGACCCCTGGGTGCCTAAGTTACCGCCACATTTGGTAAAACTCGGGCGCACTTGGGAAATAGTGCGATTGGCGTTATCGCTTAAACATTCCACCATCACTGCCGCACCGCCCGGACCGTAACCTTCATACACGCGGGTTTCCATGTTGGTATCATCGCCACCGCCGACGCCGCGCTCAATCGCACGGTTAATGGTATCACGGGTCATATTATTg                                                                                                                                                                                                                       <  1:427912/251‑1 (MQ=255)                             aaaaaTCAAGCCTTTTTTACTAAACAAATAGCCCACGGACCCCTCGGTGCCTAAGTTACCGCCACATTTGGTAAAACTCGGGCGCACTTGGGAAATAGTGCGATTGGCGTTATCGCTTAAACATTCCACCATCACTGCCGCACCGCCCGGACCGTAACCTTCATACACGCGGGTTTCCATGTTGGTATCATCGCCACCGCCGACGCCGCGCTCAATCGCACGGTTAATGGTATCACGGGTCATATTATTGc                                                                                                                                                                                                                      <  1:279290/250‑1 (MQ=255)                                    aaGCCTTTTTTGCTAAAAAAATGGCCCACGGAACCCTGGGTGCTTAGGTTACCGCCACATTTGGTAAAACTCGGGCGCACTTGGGAAATAGTGCGATTGGCGTTATCGCTTAAACATTCCACCATCACTGCCGCACCGCCCGGACCGTAACCTTCATACACGCGGGTTTCCATGTTGGTATCATCGCCACCGCCGACGCCGCGCTCAATCGCACGGTTAATGGTATCACGGGTCATATTATTGCTTAATGc                                                                                                                                                                                                               <  1:591004/251‑1 (MQ=255)                                       ccTTTTTTGCAAAACAAATAGCCCACGGACCCCTCGATGCCTAAATTACCGCCACATTTGGTAAAACACGGGCGCACTTGGGAAATAGTGCGATTGGCGTTATCGCTTAAACATACCGCCATCTCTGCCGCACCGCCCGGACCGTAACCTTCATACACGCGGGTTTCCATGTTGGTATCATCGCCGCCGCCGACGCCGCGCTCAATCGCACGGTTAATGGt                                                                                                                                                                                                                                          <  1:4248/221‑1 (MQ=255)                                         ttttttACTAAACAAATAGCCCACGGACCCCTCGGTGCCTAAGTTACCGCCACATTTGGTAAAACTCGGGCGCACTTGGGAAATAGTGCGATTGGCGTTATCGCTTAAACATTCCACCATCACTGCCGCACCGCCCGGACCGTAACCTTCATACACGCGGGTTTCCATGTTGGTATCATCGCCACCGCCGACGCCGCGCTCAATCGCACGGTTAATGGTATCACGGGTCATATTATTGCTTAATGCTTTAt                                                                                                                                                                                                          <  1:320211/251‑1 (MQ=255)                                         ttttttACTAAACAAATAGCCCACGGAACCCTCGGTGCCTAAGTTACCGCCACATTTGGTAAAACTCGGGCGCACTTGGGAAATAGTGCGATTGGCGTTATCGCTTAAACATTCCACCATCACTGCCGCACCGCCCGGACCGTAACCTTCATACACGCGGGTTTCCATGTTGGTATCATCGCCACCGCCGACGCCGCGCTCAATCGCACGGTTAATGGTATCACGGGTCATATTATTGCTTAATGCTTTAt                                                                                                                                                                                                          <  1:616429/251‑1 (MQ=255)                                           ttttAATAAACAAATAGCCCACGGACCCCTCGGTGCCTAGGTTACCGCCACATTTGGTAAAACTCGGGCGCACTTGGGAAATAGTGCGATTGGCGTTATCGCTTAAACATTCCACCATCACTGCCGCACCGCCCGGACCGTAACCTTCATACACGCGGGTTTCCATGTTGGTATCATCGCCACCGCCGACGCCGCGCTCAATCGCACGGTTAATGGTATCACGGGTCATATTATTGCTTAATGCTTTATCt                                                                                                                                                                                                        <  1:127339/251‑1 (MQ=255)                                                     cAAATAGCCCACGGAACCCTCGGTGCCTAAGTTACCGCCACATTTGGTAAAACTCGGGCGCACTTGGGAAATAGTGCGATTGGCGTTATCGCTTAAACATTCCACCATCACTGCCGCACCGCCCGGACCGTAACCTTCATACACGCGGGTTTCCATGTTGGTATCATCGCCACCGCCGACGCCGCGCTCAATCGCACGGTTAATGGt                                                                                                                                                                                                                                          >  1:677653/1‑207 (MQ=255)                                                            cccccGGAACCCTCGGTGCCTAAGTTACCGCCACATTTGGTAAAACTCGGGCGCACTTGGGAAATAGTGCGATTGGCGTTATCGCTTAAACATTCCACCATCACTGCCGCACCGCCCGGACCGTAACCTTCATACACGCGGGTTTCCATGTTGGTATCATCGCCACCGCCGACGCCGCGCTCAATCGCACGGTTAATGGTATCACGGGTCATATTATTGCTTAATGCTTTATCTACCGCCGCACGCAGAcg                                                                                                                                                                                       <  1:895394/247‑1 (MQ=255)                                                            cccACGGAACCCTCGGTGCCTAAGTTACCGCCACATTTGGTAAAACTCGGGCGCACTTGGGAAATAGTGCGATTGGCGTTATCGCTTAAACATTCCACCATCACTGCCGCACCGCCCGGACCGTAACCTTCATACACGCGGGTTTCCATGTTGGTATCATCGCCACCGCCGACGCCGCGCTCAATCGCACGGTTAATGGt                                                                                                                                                                                                                                          >  1:678969/1‑200 (MQ=255)                                                               aCGGACCCCTCGGTGCCTAAGTTACCGCCACATTTGGTAAAACTCGGGCGCACTTGGGAAATAGTGCGATTGGCGTTATCGCTTAAACATTCCACCATCACTGCCGCACCGCCCGGACCGTAACCTTCATACACGCGGGTTTCCATGTTGGTATCATCGCCACCGCCGACGCCGCGCTCAATCGCACGGTTAATGGt                                                                                                                                                                                                                                          <  1:144754/197‑1 (MQ=255)                                                               aCGGAACCCTCGGTGCCTAAGTTACCGCCACATTTGGTAAAACTCGGGCGCACTTGGGAAATAGTGCGATTGGCGTTATCGCTTAAACATTCCACCATCACTGCCGCACCGCCCGGACCGTAACCTTCATACACGCGGGTTTCCATGTTGGTATCATCGCCACCGCCGACGCCGCGCTCAATCGCACGGTTAATgg                                                                                                                                                                                                                                           <  1:864384/196‑1 (MQ=255)                                                                       cTCGGTGCCTAATTTACCGCCACATTTGGTAAAACTCGGGCGCACTTGGGAAATAGTGCGATTGGCGTTATCGCTTAAACATTCCACCATCACTGCCGCACCGCCCGGACCGTAACCTTCATACACGCGGGTTTCCATGTTGGTATCATCGCCACCGCCGACGCCGCGCTCAATCGCACGGTTAATGGTATCACGGGTCATATTATTGCTTAATGCTTTATCTACCGCCGCACGCAGACGCGGGTTAGAAc                                                                                                                                                                            <  1:694619/251‑1 (MQ=255)                                                                           gTGCCTAAGTTCCCGCCACATTTGGTAAAACTCGGGCGCACTTGGGAAATAGTGCGATTGGCGTTATCGCTTGAACATTCCACCATCACTGCCGCACCGCCCGGACCGTAACCTTCATACACGCGGGTTTCCATGTTGGTATCATCGCCACCGCCGACGCCGCGCTCAATCGCACGGTTAATGGTATCACGGGTCATATTATTGCTTAATGCTTTATCt                                                                                                                                                                                                        <  1:762666/219‑1 (MQ=255)                                                                           gTGCCTAAGTTACCGCCACATTTGGTAAAACTCGGGCGCACTTGGGAAATAGTGCGATTGGCGTTATCGCTTAAACATTCCACCATCACTGCCGCACCGCCCGGACCGTAACCTTCATACACGCGGGTTTCCATGTTGGTATCATCGCCACCGCCGACGCCGCGCTCAATCGCACGGTTAATGGt                                                                                                                                                                                                                                          <  1:148332/185‑1 (MQ=255)                                                                           gTGCCTAAGTTACCGCCACATTTGGTAAAACTCGGGCGCACTTGGGAAATAGTGCGATTGGCGTTATCGCTTAAACATTCCACCATCACTGCCGCACCGCCCGGACCGTAACCTTCATACACGCGGGTTTCCATGTTGGTATCATCGCCACCGCCGACGCCGCGCTCAATCGCACGGTTAATGGTATCACg                                                                                                                                                                                                                                    >  1:386621/1‑191 (MQ=255)                                                                           gTGCCTAAGTTACCGCCACATTTGGTAAAACTCGGGCGCACTTGGGAAATAGTGCGATTGGCGTTATCGCTTAAACATTCCACCATCACTGCCGCACCGCCCGGACCGTAACCTTCATACACGCGGGTTTCCATGTTGGTATCATCGCCACCGCCGACGCCGCGCTCAATCGCACGGTTAATGGTATCACGGGTCATATTATTGCTTAATGc                                                                                                                                                                                                               >  1:69650/1‑212 (MQ=255)                                                                           gTGCCTAAGTTACCGCCACATTTGGTAAAACTCGGGCGCACTTGGGAAATAGTGCGATTGGCGTTATCGCTTAAACATTCCACCATCACTGCCGCACCGCCCGGACCGTAACCTTCATACACGCGGGTTTCCATGTTGGTATCATCGCCACCGCCGACGCCGCGCTCAATCGCACGGTTAATGGTATCACGGGTCATATTATTGCTTAATGCTTTATCTACCGCCGCACGCAGACGCGGGTTAGAACTaa                                                                                                                                                                         >  1:587928/1‑250 (MQ=255)                                                                            tGCCTAAGTTCCCGCCACATTTGGTAAAACTCGGGCGCACTTGGGAAATAGTGCGATTGGCGTTATCGCTTAAACATTCCACCATCACTGCCGCACCGCCCGGACCGTAACCTTCATACACGCGGGTTTCCATGTTGGTATCATCGCCACCGCCGACGCCGCGCTCAATCGCACGGTTAATGGTATCACGGGTCATATTATTGCTTAATGCTTTATCTACCGCCGCACGCAGACGCGGGTTAGAACTAACa                                                                                                                                                                       <  1:754015/251‑1 (MQ=255)                                                                             gCCTAATTTACCGCCACATTTGGTAAAACTCGGGCGCACTTGGGAAATAGTGCGATTGGCGTTATCGCTTAAACATTCCACCATCACTGCCGCACCGCCCGGACCGTAACCTTCATACACGCGGGTTTCCATGTTGGTATCATCGCCACCGCCGACGCCGCGCTCAATCGCACGGTTAATGGTATCACGGGTCAtatt                                                                                                                                                                                                                           <  1:768413/198‑1 (MQ=255)                                                                                        cGCCACATTTGGTAAAACTCGGGCGCACTTGGGAAATAGTGCGATTGGCGTTATCGCTTAAACATTCCACCATCACTGCCGCACCGCCCGGACCGTAACCTTCATACACGCGGGTTTCCATGTTGGTATCATCGCCACCGCCGACGCCGCGCTCAATCGCACGGTTAATGGTATCACGGGTCATATTATTGCTTAATGCTTTATCTACCGCCGCACGCAGACGCGGGTTAGAACTAACATCACCGCCACCg                                                                                                                                                           <  1:639168/251‑1 (MQ=255)                                                                                                  ggTAAAACTCGGGCGCACTTGGGAAATAGTGCGATTGGCGTTATCGCTTAAACATTCCACCATCACTGCCGCACCGCCCGGACCGTAACCTTCATACACGCGGGTTTCCATGTTGGTATCATCGCCACCGCCGACGCCGCGCTCAATCGCACGGTTAATGGTATCACGGGTCATATTATTGCTTAATGCTTTATCTACCGCCGCACGCAGACGCGGGTTAGAACTAACATCACCGCCACCGATTTTAGCgg                                                                                                                                                 >  1:901140/1‑251 (MQ=255)                                                                                                                 cACTTGGGAAATAGTGCGATTGGCGTTATCGCTTAAACATTCCACCATCACTGCCGCACCGCCCGGACCGTAACCTTCATACACGCGGGTTTCCATGTTGGTATCATCGCCACCGCCGACGCCGCGCTCAATCGCACGGTTAATGGTATCACGGGTCATATTATTGCTTAATGCTTTATCTACCGCCGCACGCAGACGCGGGTTAGAACTAACATCACCGCCACCGATTTTAGCGGCAGTCACCAATTCa                                                                                                                                   >  1:271354/1‑250 (MQ=255)                                                                                                                  gcTTGGGAAATAGTGCGATTGGCGTTATCGCTTAAACATTCCACCATCACTGCCGCACCGCCCGGACCGTAACCTTCATACACGCGGGTTTCCATGTTGGTATCATCGCCACCGCCGACGCCGCGCTCAATCGCACGGTTAATGGTATCACGGGTCAtatt                                                                                                                                                                                                                           <  1:475878/160‑1 (MQ=255)                                                                                                                   cTTGGGAAATAGTGCGATTGGCGTTATCGCTTAAACATTCCACCATCACTGCCGCACCGCCCGGACCGTAACCTTCATACACGCGGGTTTCCATGTTGGTATCATCGCCACCGCCGACGCCGCGCTCAATCGCACGGTTAATGGTATCACGGGTCATATTATTGCTTAATGCTTTATCTACCGCCGCACGCAGACGCGGGTTAGAACTAACa                                                                                                                                                                       <  1:494587/212‑1 (MQ=255)                                                                                                                   cTTGGGAAATAGTGCGATTGGCGTTATCGCTTAAACATTCCACCATCACTGCCGCACCGCCCGGACCGTAACCTTCATACACGCGGGTTTCCATGTTGGTATCATCGCCACCGCCGACGCCGCGCTCAATCGCACGGTTAATGGTATCACGGGTCATATTATTGCTTAATGCTTTATCTACCGCCGCACGCAGACGCGGGTTAGAACTAACATCACCGCCACCGATTTTAGCGGCAGTCACCAATTCACGa                                                                                                                                >  1:718893/1‑251 (MQ=255)                                                                                                                   cTTGGGAAATAGTGCGATTGGCGTTATCGCTTAAACATTCCACCATCACTGCCGCACCGCCCGGACCGTAACCTTCATACACGCGGGTTTCCATGTTGGTATCATCGCCACCGCCGACGCCGCGCTCAATCGCACGGTTAATGGTATCACGGGTCATATTATTGCTTAATGCTTTATCTACCGCCGCACGCAGACGCGGGTTAGAACTAACATCACCGCCACCGATTTTAGCGGCAGTCACCAATTCACGa                                                                                                                                >  1:350147/1‑251 (MQ=255)                                                                                                                    ttGGGAAATAGTGCGATTGGCGTTATCGCTTAAACATTCCACCATCACTGCCGCACCGCCCGGACCGTAACCTTCATACACGCGGGTTTCCATGTTGGTATCATCGCCACCGCCGACGCCGCGCTCAATCGCACGGTTAATGGt                                                                                                                                                                                                                                          >  1:10357/1‑144 (MQ=255)                                                                                                                      gggAAATAGTGCGATTGGCGTTATCGCTTAAACATTCCACCATCACTGCCGCACCGCCCGGACCGTAACCTTCATACACGCGGGTTTCCATGTTGGTATCATCGCCACCGCCGACGCCGCGCTCAATCGCACGGTTAATGGt                                                                                                                                                                                                                                          <  1:775698/142‑1 (MQ=255)                                                                                                                        gAAATAGTGCGATTGGCGTTATCGCTTAAACATTCCACCATCACTGCCGCACCGCCCGGACCGTAACCTTCATACACGCGGGTTTCCATGTTGGTATCATCGCCACCGCCGACGCCGCGCTCAATCGCACGGTTAATGGt                                                                                                                                                                                                                                          >  1:245407/1‑140 (MQ=255)                                                                                                                                 cGATTGGCGTTATCGCTTAAACATTCCACCATCACTGCCGCACCGCCCGGACCGTAACCTTCATACACGCGGGTTTCCATGTTGGTATCATCGCCACCGCCGACGCCGCGCTCAATCGCACGGTTAATGGTATCACGGGTCATATTATTGCTTAATGCTTTATCTACCGCCGCACGCAGACGCGGGTTAGAACTAACa                                                                                                                                                                       <  1:420318/198‑1 (MQ=255)                                                                                                                                                          ccaccaTCACTGCCGCACCGCCCGGACCGTAACCTTCATACACGCGGGTTTCCATGTTGGTATCATCGCCACCGCCGACGCCGCGCTCAATCGCACGGTTAATGGTATCACGGGTCATATTATTGCTTAATGCTTTATCTACCGCCGCACGCAGACGCGGGTTAGAACTAACATCACCGCCACCGATTTTAGCGGCAGTCACCAATTCACGAATTAATTTGGTAAAAATTTTCCCGCGCTGAGCATCTtgt                                                                                         >  1:485594/1‑251 (MQ=255)                                                                                                                                                                 cACTGCCGCACCGCCCGGACCGTAACCTTCATACACGCGGGTTTCCATGTTGGTATCATCGCCACCGCCGACGCCGCGCTCAATCGCACGGTTAATGGTATCACGGGTCATATTATTGCTTAATGCTTTATCTACCGCCGCACGCAGACGCGGGTTAGAACTAACATCACCGCCACCGATTTTAGCGGCAGTCACCAATTCACGAATTAATTTGGTAAAAATTTTCCCGCGCTGAGCATCTTGTGCCGCtt                                                                                  >  1:884108/1‑251 (MQ=255)                                                                                                                                                                   cTGCCGCACCGCCCGGACCGTAACCTTCATACACGCGGGTTTCCATGTTGGTATCATCGCCACCGCCGACGCCGCGCTCAATCGCACGGTTAATGGTATCACGGGTCATATTATTGc                                                                                                                                                                                                                      <  1:603585/117‑1 (MQ=255)                                                                                                                                                                   cTGCCGCACCGCCCGGACCGTAACCTTCATACACGCGGGTTTCCATGTTGGTATCATCGCCACCGCCGACGCCGCGCTCAATCGCACGGTTAATGGTATCACGGGTCATATTATTGCTTAATGCTTTATCTAccgc                                                                                                                                                                                                   >  1:795072/1‑136 (MQ=255)                                                                                                                                                                      ccGCACCGCCCGGACCGTAACCTTCATACACGCGGGTTTCCATGTTGGTATCATCGCCACCGCCGACGCCGCGCTCAATCGCACGGTTAATGGTATCACGGGTCATATTATTGCTTAATGCTTTATCTACCGCCGCACGCAGACGCGGGTTAGAACTAACATCACCGCCACCGATTTTAGCGGCAGTCACCAATTCACGAATTAATTTGGTAAAAATTTTCCCGCGCTGAGCATCTTGTGCCGCTTTACgg                                                                             <  1:743674/251‑1 (MQ=255)                                                                                                                                                                         cACCGCCCGGACCGTACCCTTCATACACGCGGGTTTCCATGTTGGTATCATCGCCACCGCCGACGCCGCGCTCAATCGCACGGTTAATGGTATCACGGGTCATATTATTGCTTAATGCTTTATCTACCGCCGCACGCAGACGCGGGTTAGAACTAACATCACCGCCACCGATTTTAGCGGCAGTCACCAATTCACGAATTAATTTGGTAAAAATTTTCCCGCGCTGAGCATCTTGTGCCGCTTTACGgtgt                                                                          <  1:366974/251‑1 (MQ=255)                                                                                                                                                                             gCCCGGACCGTAACCTTCATACACGCGGGTTTCCATGTTGGTATCATCGCCACCGCCGACGCCGCGCTCAATCGCACGGTTAATGGTATCACGGGTCATATTATTGCTTAATGCTTTATCTACCGCCGCACGCAGACGCGGGTTAGAACTAACATCACCGCCACCGATTTTAGCGGCAGTCACCAATTCACGAATTAATTTGGTAAAAATTTTCCCGCGCTGAGCATCTTGTGCCGCTTTACGGTGTTTaa                                                                      >  1:422152/1‑251 (MQ=255)                                                                                                                                                                              cccGGACCGTAACCTTCATACACGCGGGTTTCCATGTTGGTATCATCGCCGCCGCCGACGCCGCGCTCAATCGCACGGTTAATGGTATCACGGGTCATATTATTGCTTAATGCTTTATCTACCGCCGCACGCAGACGCGGGTTAGAACTAACATCACCGCCACCGATTTTAGCGGCAGTCACCAATTCACGAATTAATTTGGTAAAAATTTTCCCGCGCTGAGCATCTTGTGCCGCTTTACGGTGTTTAat                                                                     <  1:863062/251‑1 (MQ=255)                                                                                                                                                                               ccGGACCGTAACCTTCATACACGCGGGTTTCCATGTGGGTATCATCGCCACCGCCGACGCCGCGCTCAATCGCACGGTTAATGGTATCACGGGTCATATTATTGCTTAATGCTTTATCTACCGCCGCACGCAGACGCGGGTTAGAACTAACATCACCGCCACCGATTTTAGCGGCAGTCACCAATTCACGAATTAATTTGGTAAAAATTTTCCCGCGCTGAGCATCTTGTGCCGCTTTACGGTGTTTAata                                                                    <  1:417987/251‑1 (MQ=255)                                                                                                                                                                                cGGACCGTAACCTTCATACACGCGGGTTTCCATGTTGGTATCATCGCCACCGCCGACGCCGCGCTCAATCGCACGGTTAATGGTATCACGGGTCATATTATTGCTTAATGCTTTATCTACCGCCGCACGCAGACGCGGGTTAGAACTAACATCACCGCCACCGATTTTAGCGGCAGTCACCAATTCACGAATTAATTTGGTAAAAATTTTCCCGCGCTGAGCATCTTGTGCCGCTTTACGGTGTTTAatat                                                                   <  1:201142/251‑1 (MQ=255)                                                                                                                                                                                    ccGTAACCTTCATACACGCGGGTTTCCATGTTGGTATCATCGCCACCGCCGACGCCGCGCTCAATCGCACGGTTAATGGTATCACGGGTCATATTATTGCTTAATGCTTTATCTACCGCCGCACGCAGACGCGGGTTAGAACTAACATCACCGCCACCGATTTTAGCGGCAGTCACCAATTCACGAATTAATTTGGTAAAAATTTTCCCGCGCTGAGCATCTTGTGCCGCTTTACGGTGTTTAATATTTg                                                                >  1:559116/1‑250 (MQ=255)                                                                                                                                                                                          ccTTCATACACGCGGGTTTCCATGTTGGTATCATCGCCACCGCCGACGCCGCGCTCAATCGCACGGTTAATGGTATCACGGGTCATATTATTGCTTAATGCTTTATCTACCGCCGCACGCAGACGCGGGTTAGAACTAACATCACCGCCACCGAtt                                                                                                                                                        >  1:571076/1‑156 (MQ=255)                                                                                                                                                                                              cATACACGCGGGTTTCCATGTTGGTATCATCGCCACCGCCGACGCCGCGCTCAATCGCACGGTTAATGGTATCACGGGTCATATTATTGCTTAATGCTTTATCTACCGCCGCACGCAGACGCGGGTTAGAACTAACATCACCGCCACCGATTTTAGCGGCAGTCACCAATTCACGAATTAATTTGGTAAAAATTTTCCCGCGCTGAGCATCTTGTGCCGCTTTACGGTGTTTAATATTTGCCCACTTACTa                                                     <  1:220621/251‑1 (MQ=255)                                                                                                                                                                                                        ggTTTCCATGTTGGTATCATCGCCACCGCCGACGCCGCGCTCAATCGCACGGTTAATGGTATCACGGGTCATATTATTGCTTAATGCTTTATCTACCGCCGCACGCAGACGCGGGTTAGAACTAACATCACCGCCACCGATTTTAGCGGCAGTCACCAATTc                                                                                                                                    >  1:108690/1‑162 (MQ=255)                                                                                                                                                                                                             ccATGTTGGTATCATCGCCACCGCCGACGCCGCGCTCAATCGCACGGTTAATGGTATCACGGGTCATATTATTGCTTAATGCTTTATCTACCGCCGCACGCAGACGCGGGTTAGAACTAACATCACCGCCACCGATTTTAGCGGCAGTCACCAATTCACGAATTAATTTGGTAAAAATTTTCCCGCGCTGAGCATCTTGTGCCGCTTTACGGTGTTTAATATTTGCCCACTTACTATGGCCTGCCATTTTg                                      >  1:265675/1‑251 (MQ=255)                                                                                                                                                                                                               aTGTTGGTATCATCGCCACCGCCGACGCCGCGCTCAATCGCACGGTTAATGGTATCACGGGTCATATTATTGCTTAATGCTTTATCTACCGCCGCACGCAGACGCGGGTTAGAACTAACATCACCGCCACCGATTTTAGCGGCAGTCACCAATTCACGAATTAAttt                                                                                                                        <  1:287233/167‑1 (MQ=255)                                                                                                                                                                                                                   tGGTATCATCGCCACCGCCGACGCCGCGCTCAATCGCACGGTTAATGGTATCACGGGTCATATTATTGCTTAATGCTTTATCTACCGCCGCACGCAGACGCGGGTTAGAACTAACATCACCGCCACCGATTTTAGCGGCAGTCACCAATTCACGAATTAATTTGGTAAAAATTTTCCCGCGCTGAGCATCTTGTGCCGCTTTACGGTGTTTAATATTTGCCCACTTACTATGGCCTGCCATTTTGTATTcc                                <  1:681586/251‑1 (MQ=255)                                                                                                                                                                                                                     gTATCATCGCCACCGCCGACGCCGCGCTCAATCGCACGGTTAATGGTATCACGGGTCATATTATTGCTTAATGCTTTATCTACCGCCGCACGCAGACGCGGGTTAGAACTAACATCACCGCCACCGATTTTAGCGGCAGTCACCAATTCACGAATTAATTTGGTAAAAATTTTCCCGCGCTGAGCAt                                                                                              <  1:126089/187‑1 (MQ=255)                                                                                                                                                                                                                                  cgccgACGCCGCGCTCAATCGCACGGTTAATGGTATCACGGGTCATATTATTGCTTAATGCTTTATCTACCGCCGCACGCAGACGCGGGTTAGAACTAACATCACCGCCACCGATTTTAGCGGCAGTCACCAATTCACGAATTAATTTGGTAAAAATTTTCCCGCGCTGAGCATCTTGTGCCGCTTTACGGTGTTTAATATTTGCCCACTTACTATGGCCTGCCATTTTGTATTCCTTCTCAAtttattta                 >  1:337359/1‑251 (MQ=255)                                                                                                                                                                                                                                                 cAATCGCACGGTTAATGGTATCACGGGTCATATTATTGCTTAATGCTTTATCTACCGCCGCACGCAGACGCGGGTTAGAACTAACATCACCGCCACCGATTTTAGCGGCAGTCACCAATTCACGAATTAATTTGGTAAAAATTTTCCCGCGCTGAGCATCTTGTGCCGCTTTACGGTGTTTAATATTTGCCCACTTACTATGGCCTGCCATTTTGTATTCCTTCTCAATTTATTTAAATTCGGCTTATTTc  <  1:629516/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     TCAAAGCGTCTTCATCCGCCTGATTCACTAAAATCAAGCCTTTTTTACTAAACAAATAGCCCACGGAACCCTCGGTGCCTAAGTTACCGCCACATTTGGTAAAACTCGGGCGCACTTGGGAAATAGTGCGATTGGCGTTATCGCTTAAACATTCCACCATCACTGCCGCACCGCCCGGACCGTAACCTTCATACACGCGGGTTTCCATGTTGGTATCATCGCCACCGCCGACGCCGCGCTCAATCGTACGGTTAATGGTATCACGGGTCATATTATTGCTTAATGCTTTATCTACCGCCGCACGCAGACGCGGGTTAGAACTAACATCACCGCCACCGATTTTAGCGGCAGTCACCAATTCACGAATTAATTTGGTAAAAATTTTCCCGCGCTGAGCATCTTGTGCCGCTTTACGGTGTTTAATATTTGCCCACTTACTATGGCCTGCCATTTTGTATTCCTTCTCAATTTATTTAAATTCGGCTTATTTC  >  1/572272‑572762

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑