| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | 1 | 641,384 | 0 | C | A | 37.7% | 105.5 / 27.3 | 69 | V90F (GTT→TTT) | unknown | Probable 3‑phenylpropionic acid transporter |
| Reads supporting (aligned to +/- strand): ref base C (30/13); new base A (0/26); total (30/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.22e-09 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCGTATAAGCGGCTAAAAGTGCGGCCAAAATCCAAACGATATTTTGCTCGCCGAAAAAGCCGATCACATTGCCGAACAAAGTCACGCCCACCACAAACGCCAGAGAACCGATTAA‑CCGCGCTTTGCCGTAATCCAGTTGAATCTGGCGCTGCCAGGTGCTCGCCAGCGAATCCGTAATCGGCATACCGGCGGCGTTTACCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TTCTCGGCAACAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGCACCATGCCGATCATTTCCTCGCCGTATTGTTGGGATTTCATCCAAACGGGAAAAAACGGCACGAATACGCCATAAGCGCAGTAGTAGCCGAAAAAG > 1/641135‑641616 | gCGTATAAGCGGCTAAAAGTGCGGCCAAAATCCAAACGATATTTTGCTCGCCGAAAAAGCCGATCACATTGCCGAACAAAGTCACGCCCACCACAAACGCCAGAGAACCGATTAA‑CCGCGCTTTGCCGTAATCCAGTTGAATCTGGCGCTGCCAGGTGCTCGCCAGCGAATCCGTAATCGGCATACCGGCGGCGTTTACCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TTCTCGGcaaca > 1:128569/1‑251 (MQ=255)gCGTATAAGCGGCTAAAAGTGCGGCCAAAATCCAAACGATATTTTGCTCGCCGAAAAAGCCGATCACATTGCCGAACAAAGTCACGCCCACCACAAACGCCAGAGAACCGATTAA‑CCGCGCTTTGCCGTAATCCAGTTGAATCTGGCGCTGCCAGGTGCTCGCCAGCGAATCCGTAATCGGCATACCGGCGGCGTTTACCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TTCTCGGcaaca > 1:30472/1‑251 (MQ=255)gCGTATAAGCGGCTAAAAGTGCGGCCAAAATCCAAACGATATTTTGCTCGCCGAAAAAGCCGATCACATTGCCGAACAAAGTCACGCCCACCACAAACGCCAGAGAACCGATTAA‑CCGCGCTTTGCCGTAATCCAGTTGAATCTGGCGCTGCCAGGTGCTCGCCAGCGAATCCGTAATCGGCATACCGGCGGCGTTTACCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TTCTCGGcaaca > 1:160405/1‑251 (MQ=255)gCGTATAAGCGGCTAAAAGTGCGGCCAAAATCCAAACGATATTTTGCTCGCCGAAAAAGCCGATCACATTGCCGAACAAAGTCACGCCCACCACAAACGCCAGAGAACCGATTAA‑CCGCGCTTTGCCGTAATCCAGTTGAATCTGGCGCTGCCAGGTGCTCGCCAGCGAATCCGTAATCGGCATACCGGCGGCGTTTACCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TTCTCGGcaaca > 1:245522/1‑251 (MQ=255)gCGTATAAGCGGCTAAAAGTGCGGCCAAAATCCAAACGATATTTTGCTCGCCGAAAAAGCCGATCACATTGCCGAACAAAGTCACGCCCACCACAAACGCCAGAGAACCGATTAA‑CCGCGCTTTGCCGTAATCCAGTTGAATCTGGCCCTGCCAGGTGCTCGCCAGCGAATCCGTAATCGGCATACCGGCGGCGTTTACCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TTCTCGGcaaca > 1:227188/1‑251 (MQ=255) ataAGCGGCTAAAAGTGCGGCCAAAATCCAAACGATATTTTGCTCGCCGAAAAAGCCGATCACATTGCCGAACAAAGTCACGCCCACCACAAACGCCAGAGAACCGATTAA‑CCGCGCTTTGCCGTAATCCAGTTGAATCTGGCGCTGCCAGGTGCTCGCCAGCGAATCCGTAATCGGCATACCGGCGGCGTTTACCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TTCTCGGCAACAAAAc < 1:642865/251‑1 (MQ=255) cGGCTAAAAGTGCGGCCAAAATCCAAACGATATTTTGCTCGCCGAAAAAGCCGATCACATTGCCGAACAAAGTCACGCCCACCACAAACGCCAGAGAACCGATTAA‑CCGCGCTTTGCCGTAATCCAGTTGAATCTGGCGCTGCCAGGTGCTCGCCAGCGAATCCGTAATCGGCATACCGGCGGCGTTTACCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TTCTCGGCAACAAAACTGAG‑c < 1:830379/251‑1 (MQ=255) aaaGTGCGGCCAAAATCCAAACGATATTTTGCTCGCCGAAAAAGCCGATCACATTGCCGAACAAAGTCACGCCCACCACAAACGCCAGAGAACCGATTAA‑CCGCGCTTTGCCGTAATCCAGTTGAATCTGGCGCTGCCAGGTGCTCGCCAGCGAATCCGTAATCGGCATACCGGCGGCGTTTACCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TTCTCGGCAACAAAACTGAG‑CCCCGcc < 1:880470/251‑1 (MQ=255) aTCCAAACGATATTTTGCTCGCCGAAAAAGCCGATCACATTGCCGAACAAAGTCACGCCCACCACAAACGCCAGAGAACCGATTAA‑CCGCGCTTTGCCGTAATCCAGTTGAATCTGGCGCTGCCAGGTGCTCGCCAGCGAATCCGTAATCGGCATACCGGCGGCGTTTACCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TTCTCGGCAACAAAACTGAG‑CCCCGCCATAATc > 1:78630/1‑243 (MQ=255) aaccaatttttggcccGCCAAAAAAGCCGATCACATTGCCGAAAAAAGCCCCGCCCCCAACAAACGCAAGAGAACCGATTAA‑CCGCGCTTTGCCGTAATCCAGTTGAATCTGGCGCTGCCAGGTGCTCGCCAGCAAACCCGTAAGCGGCATCCCGGCGGCGTTTCCCATGGCAAACAAC‑CCGATAGCGGAGAAAAACAGCAAAAAT‑TTCTCGGCAAAAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGcc < 1:445954/236‑1 (MQ=255) aaaCGATATTTTGCTCGCCAAAAAACCCGATCACATGGCCAAACAAAGTCACGCCCACCAAAAACGCCAGAGACCCGATTAC‑CCGCGCTTTGCCGTAATCCAGTTGATTCTGGCGCTGCCGGGTGCTCGCCAGCAAATCCGTAATCGGCATCCCGGCGGCTTTTACCATGGCAAACAAC‑CCGATAGCGGAGAAAAACAGCAAAAAA‑TTCTCGGCAACAAACCGGAG‑CCCCGCCATAATCAAGGCACTTGcc < 1:515057/251‑1 (MQ=255) aaaCGATATTTTGCTCGCAAAAAAAGCCGATCACATTGCCAAAAAAAGTCACCCCCACCACAACCGCAGGAGAACCG‑TTAACCCGCGCTTGCCCGTAATCCAGTGGAATCGGGCGCTCCCAGTTGCTCGCCAGCGAATCCGTAATCGGCATACCGGGGGCGTTTACCATGGCAAACAAA‑CCAATAGCGGAGAATAACAGCAAAAAA‑TTCTCGGCAAAAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGcc < 1:149963/251‑1 (MQ=255) atatTTTGCTCGCCGAAAAAGCCGATCACATTGCCGAACAAAGTCACGCCCACCACAAACGCCAGAGAACCGATTAA‑CCGCGCTTTGCCGTAATCCAGTTGAATCTGGCGCTGCCAGGTGCTCGCCAGCGAATCCGTAATCGGCATACCGGCGGCGTTTACCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TTCTCGGCAACAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGCCAgcg > 1:614238/1‑250 (MQ=255) atTTTGCTCGCCGAAAAAGCCGATCACATTGCCGAACAAAGTCACGCCCACCACAAACGCCAGAGAACCGATTAA‑CCGCGCTTTGCCGTAATCCAGTTGAATCTGGCGCTGCCAGGTGCTCGCCAGCGAATCCGTAATCGGCATACCGGCGGCGTTTACCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TTCTCGGCAACAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCa > 1:345842/1‑251 (MQ=255) ttGCCCGCCAAAAAACCCGACCCCATGGCCAAAAAAAGTCCCCCCCACCACAAACGCCAGAGAACCGATTAC‑CCGCGCTTTGCCGTAACCCAGTTGAATCGGGCGCTGCCAGGTGCTCCCCAGCGAATCCGTAATCGGCATCCCGGCGGCGTTTACCAGGGCAAACAAA‑CCGATAGCGGAGAATAACAGCAAAAAA‑TTCTCGGCAAAAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATa < 1:83514/251‑1 (MQ=255) ccccGCCAAAAAACCCGACCACATTGCCAAAAAAAGCCACGCCCACCACAAACGCAAGAGACCCGATTAC‑CCGCGCTTTGCCGTAATCCAGTTGAATCGGGCGCTGCCGGGTGCCCGCCAGCAAATCCGTAATCGGCATCCCGGGGGCGTTTACCATGGAAAACAAC‑CCGATAGCGGAAAATAACACCCAAAAA‑TTCTCGGCAAAAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAg < 1:881681/248‑1 (MQ=255) ccTCGCCGAAAAAGCCGATCACATTGCCAAACAAAGCCCCGCCCACCACAACCGCCGGAAAACCGTTTAC‑CCGCGCTTGCCCGTAACCCAGTTAAATCGGGCGCGGCCAGGTGCCCGCAAGCAAATCCGAAAGGGGAAACCCGGCGGCTTTTCCCATGGCAAACAAC‑CCAATGGCGGAGAAAACCAGCAAAAAA‑TTCTCGGCAAAAAAACTGGC‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATaa < 1:257653/249‑1 (MQ=255) aaaaaaGCCGATCACATTGCCAAACAAAGTCCCGCCCACCACAAACGCCAGAGACCCGATTAC‑CCGCGCTTTGCCGTAATCCAGTTGAATCTGGCGCTGCCAGGTGCTCGCCAGCGAATCCGTAATCGGCATCCCGGCGGCTTTTACCATGGAAAACAAC‑CCGATAGCGGAGAATAACAGCAAAAAA‑TTCTCGGCAAAAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAgg < 1:373401/250‑1 (MQ=255) aaaaaGCGGATCCCATTCCCAAACAAGGCCCCGCCCACAACAACCGCAAGAGACCCG‑TTAACCCGCGTTTTGCCGTAATCCGTTTGAATCTGGCGCTGCCGGGTGCTCGCCAGCAAACCCGTAATGGGCATCCGGGGGGCTTTTACCATGGCAAACAAA‑CCGAAAGCGGAAAAAAACAGCAAAAAA‑TTCTCGGCAAAAAAACGGAC‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGa < 1:282450/251‑1 (MQ=255) cccaatcccaTTGCCAAAAAAAGCCCCGCCCACAACAACCGCCAGACAACCGATTAC‑CCGCGCTTTGCCGTAATCCAGTTGAACCGGGCGTGGCCGGGTGCTCGCCGGCGAACCCGTAATGGGCATCCCGGCGGTTTTTACCAGGGCAAACAAA‑CCGATAGGGGAAAATAACAGCAAAAAA‑TTCTCGGCAAAAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTaa < 1:417247/243‑1 (MQ=255) cgaccccatgcccaaaCAAAGCCACCCCCCCCAAAACCGCCAGAAACCCAATTAC‑CCCCCCTTTCCCGTAACCCAGTTGAATCTGGCGCTGCCAGGTCCTCGCCAGCAAACCCGTAACCGCCATCCCGGCGGCGTTTCCCATGCCAAACAAC‑CCGATAGCGGAAAATAACACCCAAAAA‑TTCTCGCCAAAAAAACGGAC‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATc < 1:508649/237‑1 (MQ=255) caaTCACATTGCCGAACAAACTCCCGCCCACCACAACCGCCAGAGACCCGATTAC‑CCGCGCTTTGCCGTAACCCAGTTGAATCTGGCGCTGCCGGGTGCCCCCCAGCGAACCCGTAATCGGCATCCCGGCGGCTTTTCCCATGGCAAACAAC‑CCGATAGCGGAAAATAACAGCCAAAAA‑TTCTCGGCAAAAAATCTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATc < 1:57739/249‑1 (MQ=255) aTCACATTGCCGAACAAAGTCACGCCCACCACAAACGCCAGAGAACCGATTAA‑CCGCGCTTTGCCGTAATCCAGTTGAATCTGGCGCTGCCAGGTGCTCGCCAGCGAATCCGTAATCGGCATACCGGCGGCGTTTACCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TTCTCGGCAACAAAACTGAG‑ccc > 1:848256/1‑200 (MQ=255) aTCACATTGCCAAAAAAAGCCCCGCCCACCACAACCGCCAGAGAACCGATTAC‑CCGCGCTTTGCCGTAATCCAGTTGAATCGGGCGCTGCCAGGTGCTCGCCAGCGAATCCGTAATCGGCATACCGGCGGCGTTTACCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCAAAAAA‑TTCTCGGCAAAAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCa < 1:173510/250‑1 (MQ=255) ccacaTGGCCGAACAAAGTCACCCCCACCAAAAACGCAAGAGAACCGATAAC‑CCGCGCTTTGCCGAAATCAAGTGAAATCTGGCGCTGCAAGGTGCTCGCCGGCAAATCCGTAACCGGCATACCGGCGGCGTTTACCATGGCAAACAAA‑CCAATGGCGGAAAATAACAGCAAAAAA‑TTCTCGGCAACAAACCTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGc < 1:812538/250‑1 (MQ=255) caTTGCCGAACAAAGTCACGCCCACCACAAACGCCAGAGAACCGATTAA‑CCGCGCTTTGCCGTAATCCAGTTGAATCTGGCGCTGCCAGGTGCTCGCCAGCGAATCCGTAATCGGCATACCGGCGGCGTTTACCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TTCTCGGCAACAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTg > 1:507127/1‑250 (MQ=255) ttGCCGAACAAAGTCACGCCCACCACAAACGCCAGAGAACCGATTAA‑CCGCGCTTTGCCGTAATCCAGTTGAATCTGGCGCTGCCAGGTGCTCGCCAGCGAATCCGTAATCGGCATACCGGCGGCGTTTACCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TTCTCGGCAACAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGa > 1:575492/1‑251 (MQ=255) cccaaaCAAAGTCCCCCCCACCACAACCGCCAGAGACCCGATTAC‑CCGCGCTTTGCCGTAATCCAGTTGAATCTGGCGCTGCCAGGTGCTCGCCAGCGAATCCGTAATCGGCATCCCGGCGGCTTTTCCCATGGCAAACAAC‑CCGATAGCGGAAAATAACAGCCAAAAA‑TTCTCGGCAAAAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACg < 1:505009/247‑1 (MQ=255) ccACGCCCACCACAAACGCAGGAGACCCGATCAG‑CCGCGTTTTGCCGTAATCCAGTGGATTCTGGCGCTGCCAGGTGCTCGCCAGCGAATCCGTAACCGGAATACGGGCGGCGTTTACCATGGCAAACAAC‑CCGATAGCGGAGAATAACAGCAAAAAA‑TTCTCGGAAACAAAACGGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTttaatt < 1:549830/250‑1 (MQ=255) ccacAAACGCCAGAGAACCGATTAA‑CCGCGCTTTGCCGTAATCCAGTTGAATCTGGCGCTGCCAGGTGCTCGCCAGCGAATCCGTAATCGGCATACCGGCGGCGTTTACCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TTCTCGGCAACAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGgag > 1:353861/1‑251 (MQ=255) ccGCCAGAAACCCGATTAC‑CCGCGCTTTGCCGTAATCCGGTGGAATCTGGCGCTGCCAGGTGCTCGCCAGCGAACCCTTAACCGGCATCCCGGCGGCTTTTACCATGGAAAACAAC‑CCGATAGCGGAAAATAACAGCAAAAAA‑TTCTCGGCAACAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAAc < 1:595519/250‑1 (MQ=255) cGCCAGAGAACCGATTAA‑CCGCGCTTTGCCGTAATCCAGTTGAATCTGGCGCTGCCAGGTGCTCGCCAGCGAATCCGTAATCGGCATACCGGCGGCGTTTACCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TTCTCGGCAACAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTttaatt > 1:295146/1‑235 (MQ=255) ccAGAGAACCGATTAA‑CCGCGCTTTGCCGTAATCCAGTTGAATCTGGCGCTGCCAGGTGCTCGCCAGCGAATCCGTAATCGGCATACCGGCGGCGTTTACCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TTCTCGGCAACAAAAc < 1:456637/156‑1 (MQ=255) gATTAA‑CCGCGCTTTGCCGTAATCCAGTTGAATCTGGCGCTGCCAGGTGCTCGCCAGCGAATCCGTAATCGGCATACCGGCGGCGTTTACCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TTCTCGGCAACAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACaa > 1:886135/1‑251 (MQ=255) gATTAA‑CCGCGCTTTGCCGTAATCCAGTTGAATCTGGCGCTGCCAGGTGCTCGCCAGCGAATCCGTAATCGGCATACCGGCGGCGTTTACCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TTCTCGGCAACAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACaa > 1:480204/1‑251 (MQ=255) ccGCGCTTTGCCGTAATCCAGTTGAATCTGGCGCTGCCAGGTGCTCGCCAGCGAATCCGTAATCGGCATACCGGCGGCGTTTACCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TTCTCGGCAACAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGa > 1:199485/1‑251 (MQ=255) ccGTAATCCAGTGGATTCGGGCGCTGCCAGGTGCTCGCAAGCGAATCCGTAATCGGCATACGGGGGGCTTTTCCCAGGGAAAAAAAA‑CCGAAAGGGGAAAAAAACAGCAAAAAA‑TTCTCGGCAAAAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAg < 1:823526/250‑1 (MQ=255) ttGAATCGGGCGCTGCCGGGTCCCCCCCAGCAAACCCGAAACCGCCATCCCGGCGGCTTTCCCCATGGAAAACAAC‑CCGATAGCGGAAAAAAACAGCAAAAAA‑TTCTCGGAAAAAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAGTTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGc < 1:775928/250‑1 (MQ=255) tggAATCTGGCGCTGCCAGGTGCTCGCCAGCGAACCCGTAACCGGCATACCGGCGGCTTTTACCAGGGCAAAAAAC‑CCGATAGCGGAGAATAACAGCAAAAAA‑TTCTCGGCAACAAAACTGAC‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGc < 1:78334/248‑1 (MQ=255) tGAATCGGGCGCTGCCGGGTGCTCGCCAGCGAATCCGTAATCGGCATCCCGGCGGCTTTTACCATGGAAAAAAAA‑CCGATAGCGGAAAAAAACAGCCAAAAA‑TTCTCGGCAAAAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGCAc < 1:716253/251‑1 (MQ=255) tGAATCGGGCGCTGCCGGGTGCTCGCCAGCAAATCCGAAATCGGCATACCGGGGGCGTTTACCAGGGCAAAAAAA‑CCGAAAGCGGAAAAAAACAGCCAAAAT‑TTCTCGGCAACAAAACGGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGCAc < 1:361923/251‑1 (MQ=255) gaaccgggcgctcccggggccccGCCAGCGAACCCGTAACCGGCATACGGGCGGCTTTTACCATGGAAAACAAC‑CCGATAGCGAAAAAAAACACCAAAAAA‑TTCTCGGCAAAAAACCGGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGCAc < 1:580713/228‑1 (MQ=255) aaatcgggcgcgcccgggggcccGCCAGCGAACCGGAAAGCGGCACACCGGGGGCGTTTACCA‑GGAAAAAAAACCCGATAGGGGAAAATAACAGCAAAAAA‑TTCTGGGAAAAAAAACGGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGCAc < 1:404887/228‑1 (MQ=255) aaTCTGGCGCTGCCAGGTGCTCGCCAGCGAATCCGTAATCGGCATACCGGCGGCGTTTACCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TTCTCGGCAACAAAACTGAG‑CCCCGCCATAATc > 1:678747/1‑133 (MQ=255) cgggCGCTGCAGGGTGCTCGCCAGCAAACCCGTAATCGGCATCCCGGCGGCTTTTACCATGGAAAAAAAC‑CCGATAGCGGAGAATAACAGCCAAAAT‑TTCTCGGCAAAAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGCACCATGc < 1:113154/249‑1 (MQ=255) cTGCCGGGTGCTCGCCAGCGACTCCGTAATCGGCATCCCGGCGGCGTTTACCATGGCAAACAAA‑CCGATAGGGGAAAATAACAGCCAAAAA‑TTCTCGGAAAAAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGa < 1:130269/218‑1 (MQ=255) tCCGTAATCGGCATACCGGCGGCGTTTACCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TTCTCGGCAACAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAgcgc > 1:128765/1‑129 (MQ=255) tCCGTAATCGGCATACCGGCGGCGTTTACCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TTCTCGGCAACAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCAc > 1:336199/1‑208 (MQ=255) cGTAATCGGCATCCCGGCGGCTTTTCCCATGGCAAACAAC‑CCGATAGCGGAGAATAACAGCCAAAAA‑TTCTCGGCAACAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATc < 1:217749/139‑1 (MQ=255) gTAATCGGCATACCGGCGGCGTTTACCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TTCTCGGCAACAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGCAc > 1:60633/1‑215 (MQ=255) gTAATCGGCATACCGGCGGCGTTTACCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TTCTCGGCAACAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGCACCATGCCGATCATTTCCTCGCCGTATTGTTGGGAttt > 1:285057/1‑251 (MQ=255) cGGCATACCGGCGGCGTTTACCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TTCTCGGCAACAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGCACCATGCCGATCATTTCCTCGCCGTATTGTGGGGATTTCATc > 1:653573/1‑250 (MQ=255) gCATACCGGCGGCGTTTACCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TTCTCGGCAACAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGCACCATGCCGATCATTTCCTCGCCGTATTGTTGGGATTTCATCCaa > 1:890094/1‑251 (MQ=255) gCATACCGGCGGCGTTTACCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TTCTCGGCAACAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGCACCATGCCGATCATTTCCTCGCCGTATTGTTGGGATTTCATCCaa > 1:297192/1‑251 (MQ=255) aTACCGGCGGCGTTTACCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TTCTCGGCAACAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGCACCATGCCGATCATTTCCTCGCCGTATTGTTGGGATTTCATCCaaa > 1:817775/1‑250 (MQ=255) gcttttACCATGGCAAACAAC‑CCGATAGCGGAAAATAACAGCAAAAAA‑TTCTCGGCAAAAAACCTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATa < 1:584144/179‑1 (MQ=255) gcgTTTACCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TTCTCGGCAACAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATc > 1:314947/1‑120 (MQ=255) cttttcccaggaaaaacaaa‑cCGATAGCGGAGAAAAACACCCAAAAT‑TTCTCGAAAACAAAACTGCG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGCACCATGCCGATCATTTCCTCGCCGTATTGTTGGGATTTCATCCAAACGGGAAAAAAc < 1:58037/238‑1 (MQ=255) cttttACCAGGGAAAACAAA‑CCAATAGAGGAAAAAAACA‑CCAAAAATTTCTCGAAAAAAAAACTGAC‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGCACCATGCCGATCATTTCCTCGCCGTATTGTTGGGATTTCATCCAAACGGGAAAAAAc < 1:738345/249‑1 (MQ=255) ttaccaggaaaaacaac‑cCGATAGCGGAGAATAACAGCAAAAAA‑TTCTCGGAAACAAAACGGGG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCa < 1:649769/172‑1 (MQ=255) atgaaaaacaac‑cCGATAGCGGAAAAAAACAGCAAAAAA‑TTCTC‑GAAAAAAAACCGGGCCCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGCACCATGCCGATCATTTCCTCGCCGTATTGTTGGGATTTCATCCAAACGGGAAAAAACGGCACGaa < 1:820685/246‑1 (MQ=255) aTGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TTCTCGGCAACAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGCACCATGCCGATCATTTCCTCGCCGTATTGTTGGGATTTCATCCAAACGGGAAAAAACGGCACGaa > 1:314164/1‑251 (MQ=255) gCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TTCTCGGCAACAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGCACCATGCCGATCATTTCCTCGCCGTATTGTTGGGATTTCATCCAAACGGGAAAAAACGGCACGAATa > 1:259002/1‑250 (MQ=255) aaaacccGAAAGCGGAAAAAAACACCCAAAAA‑TTCTCGCCAAAAAACCTGAC‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGCACCATGCCGATCATTTCCTCGCCGTATTGTTGGGATTTCATCCAAACGGGAAAAAACGGCACGAATACGCCa < 1:386832/246‑1 (MQ=255) a‑cCGATAGCGGAGAATAACAGCCAAAAA‑TTCTCGGCAACAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGCACCATGCCGATCATTTCCTCGCCGTATTGTTGGGATTTCATCCAAACGGGAAAAAACGGCACGAATACGCCATAAg > 1:152252/1‑251 (MQ=255) ccGAAAGCGGAGAAAAACACCCAAAAA‑TTCTCGGCAAAAAAACTGAC‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGCACCATGCCGATCATTTCCTCGCCGTATTGTTGGGATTTCATCCAAACGGGAAAAAACGGCACGAATACGCCATAAgc < 1:894118/251‑1 (MQ=255) cGATAGCGGAGAATAACAGCCAAAAA‑TTCTCGGCAACAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGCACCATGCCGATCATTTCCTCGCCGTATTGTTGGGATTTCATCCAAACGGGAAAAAACGGCACGAATACGCCATAAgcg > 1:830006/1‑251 (MQ=255) cGATAGCGGAAAAAAACAGCAAAAAA‑TTCTCGGAAAAAAACCGGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGCACCATGCCGATCATTTCCTCGCCGTATTGTTGGGATTTCATCCAAACGGGAAAAAACGGCACGAATACGCCATAAgcg < 1:791016/251‑1 (MQ=255) gCGGAGAATAACAGCCAAAAA‑TTCTCGGCAACAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGCACCATGCCGATCATTTCCTCGCCGTATTGTTGGGATTTCATCCAAACGGGAAAAAACGGCACGAATACGCCATAAgcg > 1:665074/1‑246 (MQ=255) ggAGAATAACAGCCAAAAA‑TTCTCGGCAACAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGCACCATGCCGATCATTTCCTCGCCGTATTGTTGGGATTTCATCCAAACGGGAAAAAACGGCACGAATACGCCATAAGCGCagtagt > 1:103334/1‑251 (MQ=255) gagaATAACAGCCAAAAA‑TTCTCGGCAACAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGCACCATGCCGATCATTTCCTCGCCGTATTGTTGGGATTTCATCCAAACGGGAAAAAACGGCACGAATACGCCATAAGCGCagtag > 1:441594/1‑249 (MQ=255) gaATAACAGCCAAAAA‑TTCTCGGCAACAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGCACCATGCCGATCATCTCCTCGCCGTATTGTTGGGATTTCATCCAAACGGGAAAAAACGGCACGAATACGCCATAAGCGCAGtagtag > 1:853748/1‑250 (MQ=255) aaccaaaaaa‑TTCTCGGCAAAAAAACGGGG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCGGCGACGCCCGTTTAATAAAACCGGAGAAAAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGAACCATGCCGATCATTTCCTCGCCGTATTGTTGGGATTTCATCCAAACGGGAAAAAACGGCACGAATACGCCATAAGCGCAGTAGTAGCCGaaaa < 1:811688/246‑1 (MQ=255) gCCAAAAA‑TTCTCGGCAACAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGCACCATGCCGATCATTTCCTCGCCGTATTGTTGGGATTTCATCCAAACGGGAAAAAACGGCACGAATACGCCATAAGCGCAGTAGTAGCCGAAAAAg > 1:253708/1‑251 (MQ=255) | GCGTATAAGCGGCTAAAAGTGCGGCCAAAATCCAAACGATATTTTGCTCGCCGAAAAAGCCGATCACATTGCCGAACAAAGTCACGCCCACCACAAACGCCAGAGAACCGATTAA‑CCGCGCTTTGCCGTAATCCAGTTGAATCTGGCGCTGCCAGGTGCTCGCCAGCGAATCCGTAATCGGCATACCGGCGGCGTTTACCATGGCAAACAAA‑CCGATAGCGGAGAATAACAGCCAAAAA‑TTCTCGGCAACAAAACTGAG‑CCCCGCCATAATCAAGGCACTTGCCAGCGCCAATAAGCGCAAGGAATTAATCAGCTGCGACGCCCGTTTAATTAAACCGGAGAAGAACAAACCGCCCACAAAACGGAACACATAAGCACTCGCCAGCACCATGCCGATCATTTCCTCGCCGTATTGTTGGGATTTCATCCAAACGGGAAAAAACGGCACGAATACGCCATAAGCGCAGTAGTAGCCGAAAAAG > 1/641135‑641616 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |