| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | 1 | 525,132 | 0 | T | A | 36.7% | 46.8 / 11.8 | 30 | intergenic (‑141/+160) | unknown/unknown | Inositol transport system sugar‑binding protein/Myo‑inositol 2‑dehydrogenase 2 (EC 1.1.1.18) |
| Reads supporting (aligned to +/- strand): ref base T (16/3); new base A (0/11); total (16/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.66e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AGCAATAACAGCTTGAGACGTTAATAGGATTCCACCACAAAGTAACGCAAGTAGTGTCTTTTTCATAATGCTACTCCCTTTTATCGGTTAAACGAATAACAACAAAAATTGAAATACTGCTATACCCCACCACAACAATCATATGCGAATATAAATTTCACCAATATACAATTT‑GAAACAAATCGTCCGTT‑GAGTATCTATATCATTAAAAGAAGATTTCCTGCAACCGCCATTTTTTGAAAGTGTGAGATAGATCTTATTTTTTGGAAGGAAAATTTAACTACTGCAAAAATAAACTAAATATTTTATTTTTAGCAAAAAAATAGGAGTCCAAAGACTCCTATGTTCATGCTCAATACGAATTCGTTACGCAACCTCTTCTACGTCTATCCAACGCTTTTGTTCACATGAAAACGCAATAGCATCCAACACGCGGGAAACCTT > 1/524926‑525369 | aaagagccaggCTTGAGACTTTAATAGGATCCCCCCACAAAGTAACGCAAGTAGTGTCTTTTTCATAATGCTACTCCCTTTTATCGTTAAAACAAATAACAACAAAAATTAAAATACTGCTATCCCCCACCACAACAATCATATGCAAATATAATTTTCACCAATAAACATTTT‑AAAACAAACCTTCCGTT‑GAGTATCTATATCATAAAAAGAAGTTTTCCTGAAACCGCCATTTTTTGAAAGTGTGagat < 1:288336/241‑1 (MQ=255) aaaTACCAGTTTGAGCCGTTAATAGGTTTCCCCCAAAAAGTAACGAAAGTAGTGTTTTTTTCATATTGCTCCTCCTTTTTATCGTTTAAACAAATAACAACAAAAATTAAAATACTCCTATCCCCCCCCAAAACAATCATATGCGAATAAAAATTTCCCCAATATAAAATTT‑AAAACAAATCGTCCGTT‑GATTATCTATATAATAAAAAAAAGTTTTCCTGCAACCGCCATTTTTTGAAAGTGTGagat < 1:868719/248‑1 (MQ=255) gCTTGAGACGTTAATAGGATTCCACCACAAAGTAACGCAAGTAGTGTCTTTTTCATAATGCTACTCCCTTTTATCGGTTAAACGAATAACAACAAAAATTGAAATACTGCTATACCCCACCACAACAATCATATGCGAATATAAATTTCACCAATATACAATTT‑GAAACAAATCGTCCGTT‑GAGTATCTATATCATTAAAAGAAGATTTCCTGCAACCGCCATTTTTTGAAAGTGTGAGATAGATCTTAtt > 1:222559/1‑251 (MQ=255) cccccacAAAGAAACGCAAGTATTTTCTTTTTAATAATGCTCCTCCCTTTAATCGATTAAACAAAAAAAAACAAAAATTAAAATTCTGCTATACCCCGCCACAACAATCATATGCGAAAATAAATTTCACCAATATA‑AATTTGGAAAAAAATCGTCCGTT‑GAGTATCTATATCATTAAAAGAAAATTTCCTACAACCGCCATTTTTTGAAAGTGTGAGATAGATCTTATTTTTTGGAAGGAAAATTTAact < 1:9863/248‑1 (MQ=255) ccaccaAAAAGAAACGAAAGTAGTTTTTTTTTCATAATGCTCCCCCTTTTTATCGGTTAAACGAAAAAAAAAAAAAATAAAAATACTGCTATACCCCCCCACAACAATCATATGAGAATATAAATTCCACAAAAAAACAATTT‑GAAACAAATCGTCCGTT‑GAGTATTTATATAATAAAAAAAAGATTTCCGGAAACCGCCATTTTTTGAAAGTGTGAGATAGATCTTATTTTTTGGAAGGAAAATTTAact < 1:540316/251‑1 (MQ=255) aaGTAACGCAAGTAGTGTCTTTTTCATAATGCTACTCCCTTTTATCGGTTAAACGAATAACAACAAAAATTGAAATACTGCTATACCCCACCACAACAATCATATGCGAATATAAATTTCACCAATATACAATTT‑GAAACAAATCGTCCGTT‑GAGTATCTATATCATTAAAAGAAGATTTCCTGCAACCGCCATTTTTTGAAAGTGTGAGATAGATCTTATTTTTTGGAAGGAAAATTTAACTACTGCaaa > 1:522467/1‑251 (MQ=255) aaGTAACCAAAGTGGTGTCTTTTTCATATTGCTACTCCCTTTTATCGTTTAAACGAAAAACAAAAAAAATAAAAATTCTGCTATCCCCCCCCACAACAATCATAGGCGAATATAAATTTCACAAATATACAATTA‑AAAACAAATGGCCCGGT‑GATTATCAATATCATAAAAAAAAGTTTCCCGGCAACCGCCATTTTTTGAAAGTGTGagat < 1:187939/212‑1 (MQ=255) gTAACGCAAGTAGTGTCTTTTTCATAATGCTACTCCCTTTTATCGGTTAAACGAATAACAACAAAAATTGAAATACTGCTATACCCCACCACAACAATCATATGCGAATATAAATTTCACCAATATACAATTT‑GAAACAAATCGTCCGTT‑GAGTATCTATATCATTAAAAGAAGATTTCCTGCAACCGCCATTTTTTGAAAGTGTGAGATAGATCTTAttt > 1:755433/1‑221 (MQ=255) agtagtGTCTTTTTCATAATGCTACTCCCTTTTATCGGTTAAACGAATAACAACAAAAATTGAAATACTGCTATACCCCACCACAACAATCATATGCGAATATAAATTTCACCAATATACAATTT‑GAAACAAATCGTCCGTT‑GAGTATCTATATCATTAAAAGAAGATTTCCTGCAACCGCCATTTTTTGAAAGTGTGAGATAGATCTTATTTTTTGGAAGGAAAATTTAACTACTGCAAAAATAAACTaa > 1:144002/1‑251 (MQ=255) cTTTTTCATAATGCTACTCCCTTTTATCGGTTAAACGAATAACAACAAAAATTGAAATACTGCTATACCCCACCACAACAATCATATGCGAATATAAATTTCACCAATATACAATTT‑GAAACAAATCGTCCGTT‑GAGTATCTATATCATTAAAAGAAGATTTCCTGCAACCGcc < 1:582233/174‑1 (MQ=255) cTTTTTCATAATGCTACTCCCTTTTATCGGTTAAACGAATAACAACAAAAATTGAAATACTGCTATACCCCACCACAACAATCATATGCGAATATAAATTTCACCAATATACAATTT‑GAAACAAATCGTCCGTT‑GAGTATCTATATCATTAAAAGAAGATTTCCTGCAACCGCCATTTTTTGAAAGTGTGAGATAGATCTTATTTTTTGGAAGGAAAATTTAACTACTGCAAAAATAAACTAAAtatttta > 1:78656/1‑251 (MQ=255) gaaaaacaaaAAAAATAAAAATACTGCAACCCCCCCCAACAACAAACATAGGCAAATATAATTTTCCCAAATATACATTTA‑GAAAAAAATC‑TCCCTTAGATTATCTATATAATAAAAAGAATATTCCCTGCAACCGCCATTTTTTGAAAGTGTGAGATAGATCTTATTTTTTGGAAGGAAAATTTa < 1:539900/182‑1 (MQ=255) aaaaaTTAAAATACTGCTATCCCCCACCACAACAATAATATGCAAAAATAAATTCCACAAATATAAAATTT‑AAAAAAAATCGTCCGTT‑GAGTATCTATATCATAAAAAGAAGATTTCCTGCAACCGCCATTTTTTGAAAGTGTGAGATAGATCTTATTTTTTGGAAGGAAAATTTAACTACTGCAAAAATAAACTAAATATTTTATTTTTAGCAAAAAAATAGGAGTCCAAAGACTCCTATGTTCATGCTc < 1:373157/251‑1 (MQ=255) aaTTGAAATACTGCTATACCCCACCACAACAATCATATGCGAATATAAATTTCACCAATATACAATTT‑GAAACAAATCGTCCGTT‑GAGTATCTATATCATTAAAAGAAGATTTCCTGCAACCGCCATTTTTTGAAAGTGTGAGATAGATCTTAttt > 1:674773/1‑156 (MQ=255) aaaTACTTCTATCCCCCACCACAAAAATCATAGGCAAATATAAATTTCACCAATATACAATTT‑GAAAAAAATCGTCCTTT‑GAGTATCTATATCATAAAAAGAAGTTTTCCTGAAACCGCCATTTTTTGAAAGTGTGAGATAGATCTTATTTTTTGGAAGGAAAATTTAACTACTGCAAAAATAAACTAAATATTTTATTTTTAGCAAAAAAATAGGAGTCCAAAGACTCCTATGTTCATGCTCAATACGaa < 1:812807/251‑1 (MQ=255) cTGCTATACCCCACCACAACAATCATATGCGAATATAAATTTCACCAATATACAATTT‑GAAACAAATCGTCCGTT‑GAGTATCTATATCATTAAAAGAAGATTTCCTGCAACCGCCATTTTTTGAAAGTGTGagatag > 1:232114/1‑137 (MQ=255) cTGCTATACCCCACCACAACAATCATATGCGAATATAAATTTCACCAATATACAATTT‑GAAACAAATCGTCCGTT‑GAGTATCTATATCATTAAAAGAAGATTTCCTGCAACCGCCATTTTTTGAAAGTGTGAGATAGATc > 1:719460/1‑140 (MQ=255) gCTATACCCCACCACAACAATCATATGCGAATATAAATTTCACCAATATACAATTT‑GAAACAAATCGTCCGTT‑GAGTATCTATATCATTAAAAGAAGATTTCCTGCAACCGCCATTTTTTGAAAGTGTGAGATAGATCTTATTTTTTGGAAGGAAAATTTAACTACTGCAAAAATAAACTAAATATTTTATTTTTAGCAAAAAAATAGGAGTCCAAAGACTCCTATGTTCATGCTCAATACGAATTCGTTa > 1:867489/1‑251 (MQ=255) gCTATACCCCACCACAACAATCATATGCGAATATAAATTTCACCAATATACAATTT‑GAAACAAATCGTCCGTT‑GAGTATCTATATCATTAAAAGAAGATTTCCTGCAACCGCCATTTTTTGAAAGTGTGAGATAGATCTTATTTTTTGGAAGGAAAATTTAACTACTGCAAAAATAAACTAAATATTTTATTTTTAGCAAAAAAATAGGAGTCCAAAGACTCCTATGTTCATGCTCAATACGAATTCGTTa > 1:846799/1‑251 (MQ=255) caacaaTCATATGCGAATATAAATTTCACCAATATACAATTT‑GAAACAAATCGTCCGTT‑GAGTATCTATATCATTAAAAGAAGATTTCCTGCAACCGCCATTTTTTGAAAGTGTGAGATAGATCTTATTTTTTGGAAGGAAAATTTAACTACTGCAAAAATAAACTAAATATTTTATTTTTAGCAAAAAAATAGGAGTCCAAAGACTCCTATGTTCATGCTCAATACGAATTCGTTACGCAACCTCTTCTa > 1:798291/1‑251 (MQ=255) caacaaCCATATGCAAATATAAATTTCCCCAATATACAATTT‑AAAAAAAATCGTCCGTT‑GATTATCTATATAATAAAAAGAAGATTCCCTGCAACCGCCATTTTTTGAAAGTGTGAGATAGATCTTATTTTTTGGAAGGAAAATTTAACTACTGCAAAAATAAACTAAATATTTTATTTTTAGCAAAAAAATAGGAGTCCAAAGACTCCTATGTTCATGCTc < 1:357227/222‑1 (MQ=255) aaaTCATATGAGAATATAAATTTCACAAATATACAATTT‑GAAACAAATCGTCCGTG‑GAGTATCTATATCATAAAAAGAAGTTTTCCTGCAACCGCCATTTTTTGAAAGTGTGAGATAGATCTTATTTTTTGGAAGGAAAATTTAACTACTGCAAAAATAAACTAAATATTTTATTTTTAGCAAAAAAATAGGAGTCCAAAGACTCCTATGTTCATGCTCAATACGAATTCGTTACGCAACCTCTTCTACGt < 1:792644/250‑1 (MQ=255) cACCAATATACAATTT‑GAAACAAATCGTCCGTT‑GAGTATCTATATCATTAAAAGAAGATTTCCTGCAACCGCCATTTTTTGAAAGTGTGAGATAGATCTTATTTTTTGGAAGGAAAATTTAACTACTGCAAAAATAAACTAAATATTTTATTTTTAGCAAAAAAATAGGAGTCCAAAGACTCCTATGTTCATGCTCAATACGAATTCGTTACGCAACCTCTTCTACGTCTATCCAACGc > 1:753846/1‑239 (MQ=255) ccAATATACAATTT‑GAAACAAATCGTCCGTT‑GAGTATCTATATCATTAAAAGAAGATTTCCTGCAACCGCCATTTTTTGAAAGTGTGAGATAGATCTTATTTTTTGGAAGGAAAATTTAACTACTGCAAAAATAAACTAAATATTTTATTTTTAGCAAAAAAATAGGAGTCCAAAGACTCCTATGTTCATGCTCAATACGAATTCGTTACGCAACCTCTTCTACGTCTATcc > 1:129333/1‑232 (MQ=255) ccAATATACAATTT‑GAAACAAATCGTCCGTT‑GAGTATCTATATCATTAAAAGAAGATTTCCTGCAACCGCCATTTTTTGAAAGTGTGAGATAGATCTTATTTTTTGGAAGGAAAATTTAACTACTGCAAAAATAAACTAAATATTTTATTTTTAGCAAAAAAATAGGAGTCCAAAGACTCCTATGTTCATGCTCAATACGAATTCGTTACGCAACCTCTTCTACGTCTATCCAACGCTTTTGTTCACATGa > 1:32871/1‑251 (MQ=255) caaaTATACAATTT‑GAAACAAATCGTCCTTT‑GAGTATCTATATCATAAAAAGAAGATTTCCTGCAACCGCCATTTTTTGAAAGTGTGAGATAGATCTTATTTTTTGGAAGGAAAATTTAACTACTGCAAAAATAAACTAAATATTTTATTTTTAGCAAAAAAATAGGAGTCCAAAGACTCCTATGTTc < 1:788322/186‑1 (MQ=255) tttt‑GAAACAAATCGTCCGTT‑GAGTATCTATATAATTAAAAGAAGTTTTCCTGCAACCGCCATTTTTTGAAAGTGTGAGATAGATCTTAttt < 1:639233/91‑1 (MQ=255) aaaaaaaaTCGTCCGTT‑GAGTATATATATCATAAAAAAAAGATTTCCTGCAACCGCCATTTTTTGAAAGTGTGAGATAGATCTTATTTTTTGGAAGGAAAATTTAACTACTGCAAAAATAAACTAAATATTTTATTTTTAGCAAAAAAATAGGAGTCCAAAGACTCCTATGTTCATGCTCAATACGAATTCGTTACGCAACCTCTTCTACGTCTATCCAACGCTTTTGTTCACATGAAAACGCAATAGCAt < 1:501722/246‑1 (MQ=255) gTATCTATATCATTAAAAGAAGATTTCCTGCAACCGCCATTTTTTGAAAGTGTGAGATAGATCTTATTTTTTGGAAGGAAAATTTAACTACTGCAAAAATAAACTAAATATTTTATTTTTAGCAAAAAAATAGGAGTCCAAAGACTCCTATGTTCATGCTCAATACGAATTCGTTACGCAACCTCTTCTACGTCTATCCAACGCTTTTGTTCACATGAAAACGCAATAGCATCCAACACGCGGGAAACCtt > 1:427275/1‑251 (MQ=255) aTCTATATCATTAAAAGAAGATTTCCTGCAACCGCCATTTTTTGAAAGTGTGAGATAGATCTTATTTTTTGGAAGGAAAATTTAACTACTGCAAAAAt > 1:245199/1‑98 (MQ=255) | AGCAATAACAGCTTGAGACGTTAATAGGATTCCACCACAAAGTAACGCAAGTAGTGTCTTTTTCATAATGCTACTCCCTTTTATCGGTTAAACGAATAACAACAAAAATTGAAATACTGCTATACCCCACCACAACAATCATATGCGAATATAAATTTCACCAATATACAATTT‑GAAACAAATCGTCCGTT‑GAGTATCTATATCATTAAAAGAAGATTTCCTGCAACCGCCATTTTTTGAAAGTGTGAGATAGATCTTATTTTTTGGAAGGAAAATTTAACTACTGCAAAAATAAACTAAATATTTTATTTTTAGCAAAAAAATAGGAGTCCAAAGACTCCTATGTTCATGCTCAATACGAATTCGTTACGCAACCTCTTCTACGTCTATCCAACGCTTTTGTTCACATGAAAACGCAATAGCATCCAACACGCGGGAAACCTT > 1/524926‑525369 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |