| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | 1 | 476,575 | 0 | C | T | 39.3% | 53.8 / 16.9 | 57 | E402K (GAA→AAA) | unknown | Replication‑associated recombination protein RarA |
| Reads supporting (aligned to +/- strand): ref base C (17/17); new base T (22/0); total (40/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.43e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GACAAAATCCAAGGATTTTGAACGTTGGCTTTGCCAATGGTACGAAGTGCAAGCGCAGCGCAGTAAAAGTAGGTCGATGCCGAAACAAACCTATTTATATCGCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGG‑AAGTAGTTTTCGCCGGCAGCGTAGGC‑GTTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGA‑TTC‑GGTTG‑CC‑AATTTTTTGGC‑GG‑CTTT‑G‑AAAGCGAGATAAACGGC‑GTTGCTTTTCGGTGCGACGGCTAAATACACAATGGCTTGCGCAATAGCGCGTTCGCCTTCGGCAGCGCCGACGCGGG > 1/476348‑476817 | gACAAAATCCAAGGATTTTGAACGTTGGCTTTGCCAATGGTACGAAGTGCAAGCGCAGCGCAGTAAAAGTAGGTCGATGCCGAAACAAACCTATTTATATCGCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGG‑GAGTAGTTTTTGCCGGGAGCGGAGGC‑GGTAGGtt > 1:33556/1‑251 (MQ=255) cAAAATCCAAGGATTTTGAACGTTGGCTTTGCCAATGGTACGAAGTGCAAGCGCAGCGCAGTAAAAGTAGGTCGATGCCGAAACAAACCTATTTATATCGCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGG‑AAGTAGTTTT‑GCCGGCAGCGTAGGC‑ggttgggtc > 1:874592/1‑241 (MQ=255) gATTTTGAACGTTGGCTTTGCCAATGGTACGAAGTGCAAGCGCAGCGCAGTAAAAGTAGGTCGATGCCGAAACAAACCTATTTATATCGCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGG‑GAGGAGGTTTCGCCGGCAGCGGAgggggttgggtcattttggggggg > 1:616605/1‑229 (MQ=255) tGCCAATGGTACGAAGTGCAAGCGCAGCGCAGTAAAAGTAGGTCGATGCCGAAACAAACCTATTTATATCGCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGG‑AAGTAGTTTTTGCCGGCAGCGGAGGC‑GTTAGGTTCATTGTGGGCGTAGCGGGATTCGGCGCCGaa > 1:633361/1‑251 (MQ=255) gCCAATGGTACGAAGTGCAAGCGCAGCGCAGTAAAAGTAGGTCGATGCCGAAACAAACCTATTTATATCGCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGG‑GAGTAGTTTTTGCCGGCAGCGGAGGC‑GTTAGGTTCATTGTGGGCGGAGCGGGATTCGGCGCCggag > 1:787716/1‑248 (MQ=255) aTGGTACGAAGTGCAAGCGCAGCGCAGTAAAAGTAGGTCGATGCCGAAACAAACCTATTTATATCGCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGG‑AAGTAGTTTTTGCCCGCAGCGGAGGG‑GGTTGGGTCATTTTGGGGGGAGCGGGATTTGGGGCCGGAGCCgg > 1:630022/1‑250 (MQ=255) aCGAAGTGCAAGCGCAGCGCAGTAAAAGTAGGTCGATGCCGAAACAAACCTATTTATATCGCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGG‑AAGTAGTTTTTGCCCGCCGCGGAGGG‑GGTTGGTTCATTGTGGGGGGAGCGGGATTCGGCGCCGGAGACGAggg > 1:651334/1‑248 (MQ=255) aaGTGCAAGCGCAGCGCAGTAAAAGTAGGTCGATGCCGAAACAAACCTATTTATATCGCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGG‑AAGTAGTTTTCTCCGGCCGCGTGGGCGGTTGGGTT‑ATTGTGGGGGGAGCGGGATTCGGCGCCGAAGCCGGGGGCtttt > 1:431522/1‑250 (MQ=255) aaGTGCAAGCGCAGCGCAGTAAAAGTAGGTCGATGCCGAAACAAACCTATTTATATCGCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGG‑AAGGAGTTTTCGCCGGCAGCGTAGGC‑GGTAGGGTTATTGTGGGCGTAGCGGGATTCGGCGCCGGAGCCGAGGGCtttt > 1:461374/1‑250 (MQ=255) gTGCAAGCGCAGCGCAGTAAAAGTAGGTCGATGCCGAAACAAACCTATTTATATCGCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGG‑AAGTAGTTTTTGCCGGCAGCGTAGGC‑GGTAGGGTCATTGTGGGCGTAGCGGGATTCGGCGCCGAAGCCGAGGGCTTTCAt > 1:58765/1‑251 (MQ=255) gTGCAAGCGCAGCGCAGTAAAAGTAGGTCGATGCCGAAACAAACCTATTTATATCGCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGG‑AAGGAGTTTTTGCCGGCAGCGTAGGC‑GGTAGGTTCATTGTGGGCGGAGCGGTATTCGgcgc > 1:45427/1‑232 (MQ=255) aTGCCGAAACAAACCTATTTATATCGCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGG‑AAGTAGTTTTTGCCCGCAGCGGAGGC‑GGTAGGGTCATTGTGGGGGGAGCGGGATTCGGGGGCGAAGCCGAGGGCTTTTATGAAGGTGGTCGGGGCGTTGCgggggtgttt > 1:114160/1‑243 (MQ=255) aacCTATTTATATCGCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGG‑GAGGAGTTTTTGCCGGCAGCGGAGGC‑GGTAGGTTCATTGTGGGGGTAGCGGGGTTTGGCGCCGCAGCCGAGGGCTTTCATGAAGCTGGTCGGGGCGTTGCGGGGATGTTCCGGGACGCCGa > 1:281754/1‑251 (MQ=255) ccTATTTATATCGCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGG‑GAGTAGTTTTTGCCGGCAGCGGAGGC‑GTTAGGGTTATTGTGGGCGGAGCGGGATTCGGCGCCGAAGCCGAGGGCTTTTATGAAGCTGGTCGGGGCGGTGCGgag > 1:139744/1‑232 (MQ=255) ccTATTTATATCGCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGG‑AAGTAGTTTTTGCCGGCAGCGGAGGC‑GGTTGGTTCATTGTGAGCGGAGCGGGATTCGGCGCCGGAGCCGAGGGCTTTCATGAAGCTGGTCGGGGGGGTGCGGGGAGGTTCAGGGACGTCggaa > 1:443315/1‑248 (MQ=255) tATTTATATCGCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTTTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGG‑AAGTAGTTTTCGCCGGCAGCGTAGGC‑GTTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATc < 1:452052/251‑1 (MQ=255) aTTTATATCGCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGG‑AAGTAGTTTTTGCCGGCAGCGGAGGC‑GTTAGGGTCATTGTGGGCGGAGCGGGATTCGGCGCCGGAGCCGAGGGCTTTCATGAAGCTGGTCGGGGGGTTGCgggggtggttcggga > 1:808891/1‑227 (MQ=255) gCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGG‑AAGTAGGTTTTGCCGGCAGCGGAGGC‑GGTTGGTTCATTTTGGGCGGAGCGGGGTTTGGCGCCGAAGCCGAGGGCTTTCATGAAGCTGGTCGGGGCGTTGCGGGGGTGTTCAGGGGCGGCGGAATcggc‑gggtttg > 1:297928/1‑246 (MQ=255) cttcttACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGG‑AAGGAGTTTTTGCCGGCAGCGGAGGC‑GGTAGGGTCATTGTGGGCGGAGCGGGATTCGGCGCCGGAGCCGAGGGCTTTCATGAAGCTGGGCGGGGCGGTGCGGGGGTGGTCAGGGACGTCGAAATcggc‑ggggtcgg > 1:31810/1‑245 (MQ=255) tcttACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGG‑AAGTAGTTTTCGCCGGCAGGGGAGGG‑GTTTGGTTCATTTTGGGGGGAGCGGGATTCGGCGCCGGAGCCGAGGGCTTTCTTTAAGCTGGGCGGGGCGTTGCGGGGATGTTCAGGGGCGTCGAAATcggc‑gggtttgggt > 1:51384/1‑243 (MQ=255) cTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGG‑GAGTAGTTTTCGCCGGCAGCGGAGGC‑GTTAGGTTCATTTTGGGGGTAGCGGGATTCGGCGCCGAAGCCGAGGGCTTTTATGAAGCTGGTCGGGGCGTTGCGGGGATGTTCAGGGGCGTCGAAATCGGC‑GGG‑TTC‑GGGTG‑CC‑CAttt > 1:212405/1‑251 (MQ=255) ttCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGG‑GAGGAGTTTTTGCCGGCCGCGTAGGC‑GGTAGGTTCATTTTGGGCGGAGCGGTATTCGGCGCCGGAGCCGAGGGCTTTCATGAAGCTGGTCGGGGCGTTGCGGGGATGTTCAGGGGCGGCGAAATcggc‑gggtttgggtggccattttt > 1:258926/1‑233 (MQ=255) gATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGG‑GAGTAGTTTTTGCCGGCAGCGTAGGC‑GGTTGGGTCATTGTGGGGGGAGCGGGATTCGGCGCCGGAGCCGAGGGCTTTCATGGAGCTGGTCGGGGGGGTGCGGGGATGTTCAGGGACGGCGAAATCGGC‑GGG‑TTC‑GGGTG‑GC‑CATTTTTTtgc‑g > 1:721673/1‑251 (MQ=255) gATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGG‑AAGTAGGTTTTGCCCGCAGCGTAGGC‑GGTTGGGTCTTTGTGGGCGGAGCGGGATTCGGCGCCGGAGCCGAGGGCCTTCATGAAGGTGGTCGGGGGGGTGCGGGGATGTTCAGGGGCGGCGAAATCGGCGGGG‑TTG‑GGTGG‑CC‑AAtttttgggg > 1:474307/1‑247 (MQ=255) tcCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGG‑AAGTAGGTTTCGCCGGCAGCGGAGGC‑GGTAGGGTCATTGTGGGCGGAGCGGGATTCGGCGCCGAAGCCGAGGGCTTTCATGAAGCTGGTCGGGGCGGTGCGGAGATGGTCAGGGGCGTCGAAATCGGC‑GGG‑TTT‑GGGTG‑CC‑CATTTTTTTGCGGG‑GTTT‑G‑‑AAGCGAGATAAAAGGC‑ggtggt > 1:83645/1‑246 (MQ=255) atcttaatTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGG‑AAGTAGTTTTCGCCGGCAGCGTAGGC‑GTTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGc < 1:494816/163‑1 (MQ=255) atcttaatTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGG‑AAGTAGGTTTCGCCGGCAGCGTAGGC‑GTTTGGTTCATTGTGGGCGTAGCGGGATTCGGCGCCGAAGCCGAGGGCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGG‑TTC‑GGGTG‑GC‑AATTTTTTTGCGGG‑CTTT‑G‑AAA‑CGAGATAAAAGGC‑GGTGCtttt > 1:514313/1‑248 (MQ=255) tGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGG‑AAGTAGTTTTTGCCGGCCGCGGAGGC‑GGTAGGTTCATTGTGGGCGTAGCGGGATTCGGCGCCGAAGCCGGGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGGGATGTTCAGGGGCGTCGAAAACGGCGGGT‑TTG‑GGTGG‑CC‑ATTTTTTTGG‑‑GG‑CTTT‑GTAAAG‑GAGATAAAAGGG‑GGTGCTTTTCTGTGGGGCGGCTaaa > 1:824603/1‑251 (MQ=255) ccTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGG‑GAGGAGGTTTCGCCCGCAGCGGAGGC‑GGTCGGTTTCTTGTTGGGGTAGCGGGATTTGGCGCCCAAGCCGAGGGCTTTTATGTAACCGGGCGGGGGGTTGCGGGGATGTTTCGGGGCGTCGAAAATGGC‑GGG‑TTT‑TGTTG‑CC‑CATTTTTTTGT‑GG‑GTTT‑T‑TAAGCGAGGTAAAAGGC‑GTTTGTTTTTGGTGCGGCGGCTGAATa > 1:359876/1‑251 (MQ=255) ggtggtCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGG‑AAGTAGGTTTCGCCGGCAGCGGAGGC‑GTTAGGGTCATTGTGGGCGTAGCGGGATTCGGCGCCGAAGCCGAGGGCTTTTATGAAGCTGGTCGGGGGGGTGCGGGGATGTTCAGGGACGTCGAAAACGGCGGGG‑TTG‑GGTGG‑CC‑ATTTTTTTGG‑‑GG‑GTTT‑G‑AAAAGGGGAGAAAAGGC‑GGTGCTTTTTGGGGCGACGGCGAAATaa > 1:887473/1‑246 (MQ=255) tggtCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGG‑AAGTAGTTTTCGCCGGCAGCGTAGGC‑GTTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGA‑TTC‑GGTTG‑CC‑AATTTTTTGGC‑GG‑CTTT‑G‑AAAGCGAGATAAACGGC‑GTTGCTTTTCGGTGCGACGGCTAAATACACAAtc < 1:848025/251‑2 (MQ=255) tggtCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGG‑AAGTAGTTTTCGCCGGCAGCGTAGGC‑GTTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGA‑TTC‑GGTTG‑CC‑AATTTTTTGGC‑GG‑CTTT‑G‑AAAGCGAGATAAACGGC‑GTTGCTTTTCGGTGCGACGGCTAAATACACAAt < 1:686629/250‑1 (MQ=255) tggtCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGG‑AAGTAGTTTTCGCCGGCAGCGTAGGC‑GTTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGA‑TTC‑GGTTG‑CC‑AATTTTTTGGC‑GG‑CTTT‑G‑AAAGCGAGATAAACGGC‑GTTGCTTTTCGGTGCGACGGCTAAATACACAAt < 1:802768/250‑1 (MQ=255) gtCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGG‑AAGTAGTTTTCGCCGGCAGCGTAGGC‑GTTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGA‑TTC‑GGTTG‑CC‑AATTTTTTGGC‑GG‑CTTT‑G‑AAAGCGAGATAAACGGC‑GTTGCTTTTCGGTGCGACGGCTAAATACACAATgg < 1:801749/250‑1 (MQ=255) tCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGG‑AAGTAGTTTTCGCCGGCAGCGTAGGC‑GTTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGA‑TTC‑GGTTG‑CC‑AATTTTTTGGC‑GG‑CTTT‑G‑AAAGCGAGATAAACGGC‑GTTGCTTTTCGGTGCGACGGCTAAATACACAATGGc < 1:298754/250‑1 (MQ=255) aaaTAGAATTGGGTGTCTTTCAGTTGTGGCGGG‑AAGTAGTTTTTGCCGGCAGCGTAGGC‑GGTAGGGTCATTGTGGGGGGAGCGGGATTCGGCGCCGAAGCCGAGGGCCTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTTAGGGACGTCGAAAACGGC‑GGATTTC‑GGTTC‑CC‑AATTTTTT‑GC‑GGCCTTG‑G‑AAAG‑GCGATAAACAGCGGGTCCTTTT‑TGTGCGACCGCTAAATACACaaaagcctgcg > 1:652753/1‑241 (MQ=255) aaaTAGAATTGGGTGTCTTTCAGTTGTGGCGGG‑AAGTAGTTTTCGCCGGCAGCGTAGGC‑GTTAGGTTCATTGTGGGCGTAGTGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGA‑TTC‑GGTTG‑CC‑AATTTTTTGGC‑GG‑CTTT‑G‑AAAGCGAGATAAACGGC‑GTTGCTTTTCGGTGCGACGGCTAAATACACAATGGCTTgcgc < 1:434644/251‑1 (MQ=255) aaaTAGAATTGGGTGTCTTTCAGTTGTGGCGGG‑AAGTAGGTTTCGCCGGCAGCGTAGGG‑GGTAGGTTCATTGTGGGGGTAGCGGTATTCGGCGCCGAAGCCGAGGGCTTTCATGAAGCTGGTCGGGGCGGTGCGGGGATGTTCAGGGACGTCGAAATCGGC‑GGG‑TTC‑GGGTG‑GC‑AATTTTTTTGC‑GG‑GTTT‑T‑GAAGCGAGATAAAAGGC‑GGTGCTTTTTTGTGCGGCGGCttaaaaaacaaatgctttgg > 1:764025/1‑231 (MQ=255) aaaTAGAATTGGGTGTCTTTCAGTTGTGGCGGG‑AAGGAGTTTTTTCCGGCAGCGGAGGC‑GTTAGGGTCATTTTGGGCGGAGCGGGATTCGGCGCCGAAGCCGAGGGCTTTTCTGAAGCTGGTCGGGGCGGTGCGGGGATGTTCAGGGGCGTCGAAATCGGC‑GGG‑GTCGGGTGG‑CA‑AATTTTTTGGG‑GG‑GTTT‑G‑AAA‑CGAGATAAAAAGC‑GGTGCTTTTTTGGGCGGCGGCTGAATACACaaaaggtttcgg > 1:73317/1‑241 (MQ=255) aaaTAGAATTGGGTGTCTTTCAGTTGTGGCGGG‑AAGGAGTTTTTGCCGGCAGCGTAGGC‑GTTAGGGTCCTTGTGGGGGTAGCGGGATTCGGCGCCGGAGCCGAGGGCTTTCATGAAGCTGGTCGGGGCGTTGCGGGGATGTTCCGGGGCGTCGGAATCGGC‑GGG‑TTC‑TGGTG‑CC‑CATTTTTTTGG‑GG‑CGTT‑G‑GAAACGAGAAAAAAGGC‑GGTGCTTTTTTGTGGGGCGGCTAAAAACACaaatgcctgcg > 1:38936/1‑241 (MQ=255) aTAGAATTGGGTGTCTTTCAGTTGTGGCGGGTAAG‑AGTTTTCGCCGGGAGCGTTGGG‑GTTAGGGTTATTGTGGGCGGAGCGGGATTTGGCGCCGAAACCGAGGGCTTTCATGAAACTGGGCGGGGGGTTGTGGGGATGGTTAGGGGCGTCGAAAACGGC‑GGG‑CTC‑GGCTG‑GC‑CATATTTTTGC‑GG‑GTTTGG‑AAAG‑GAGGTAAAAGGC‑GGTGCTTTTTGGGGGGACGGCTaaaaaaaaaaagggttgtgcaa > 1:670711/1‑232 (MQ=255) aTAGAATTGGGTGTCTTTCAGTTGTGGCGGG‑AAGGAGTTTTCGCCGGCAGCGTAGGG‑GTTAGGGTTATTTTGGGGGTAGCGGGATTCGGCGCCGAAGCCGAGGGCTTTCATGAAGCTGGTCGGGGCGTTGCGGGGATGTTCAGGGGCGTCGAAATCGGC‑GGG‑TTCGGGTGG‑CC‑ATTTTTTTGGG‑GG‑GTTT‑T‑AAA‑CGGGGTAAAAGGG‑GGTGCTTTTTGGGGCGGCGGCTAAAAAAACAAAGGCTTtcgcca > 1:667471/1‑249 (MQ=255) tAGAATTGGGTGTCTTTCAGTTGTGGCGGG‑AAGTAGTTTTCGCCGGCAGCGTAGGC‑GTTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGA‑TTC‑GGTTG‑CC‑AATTTTTTGGC‑GG‑CTTT‑G‑AAAGCGAGATAAACGGC‑GTTGCTTTTCGGTGCGACGGCTAAATACACAATGGCTTGCGCAAt < 1:416422/251‑1 (MQ=255) aGAATTGGGTGTCTTTCAGTTGTGGCGGG‑AAGTAGTTTTCGCCGGCAGCGGAGGC‑GGTAGGGTCATTGTGGGGGTAGCGGGATTTGGCGCCGAAGCCGAGGGCTTTTATGAAGCTGGTCGGGGCGGTGCGGGGATGTTCAGGGGCGTCGAAATCGGC‑GGG‑TTC‑GGGTGCCC‑AATTTTTTGG‑‑GG‑GGTT‑G‑AAAAAGAGAAAAAAGGC‑GTTGCTTTTTGGTGCGGAGGCTAAATACACAAAGGCGTGCGCaaaa > 1:533492/1‑249 (MQ=255) gAATTGGGTGTCTTTCAGTTGTGGCGGG‑GAGTAGTTTTCGCCGGCAGCGGAGGC‑GGTTGGGTCATTGTGGGGGGAGCGGGATTCGGCGCCGGAGCCGAGGGCTTTCCTGAAGCTGGTCGGGGCGTTGCGGGGGTGGTCAGGGGCGGCGAAATCGGC‑GGG‑GTT‑GGGTG‑CC‑CATTTTTTTGC‑GG‑GTTT‑T‑AAAACGGGATAAAAGGG‑GGTGCCTTTTGGTGGGACGGGTaaaaaaacaaaggcctggctctttt > 1:368570/1‑229 (MQ=255) gAATTGGGTGTCTTTCAGTTGTGGCGGG‑AAGTAGTTTTCGCCGGCAGCGTAGGC‑GTTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGA‑TTC‑GGTTG‑CC‑AATTTTTTGGC‑GG‑CTTT‑G‑AAAGCGAGATAAACGGC‑GTTGCTTTTCGGTGCGACGGCTAAATACACAAt < 1:600376/237‑1 (MQ=255) gAATTGGGTGTCTTTCAGTTGTGGCGGG‑AAGGAGTTTTTGCCGGCAGCGTAGGC‑GGTAGGGTCATtg > 1:210907/1‑67 (MQ=255) gAATTGGGTGTCTTTCAGTTGTGGCGGG‑AAGGAGTTTTCGCCGGCAGCGGAGGC‑GTTTGGGTTATTGTGGGCGTAGCGGGATTCGGCGCCGAAGCCGAGGGCTTTTATGAAGCTGGGCGGGGCGGTGCGGGGATGTTTAGGGACGTCGAAATCGGC‑GGG‑TTC‑GGGTG‑CCAAATTTTTTGCG‑GG‑CTTT‑G‑AAAG‑GAGAGAAAAGGG‑GGTGGTTTTTGGGGGGACGGCTAAAAacaaaaaagg > 1:645378/1‑233 (MQ=255) aaTTGGGTGTCTTTCAGTTGTGGCGGG‑AAGTAGTTTTCGCCGGCAGCGTAGGC‑GTTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGA‑TTC‑GGTTG‑CC‑AATTTTTTGGC‑GG‑CTTT‑G‑AAAGCGAGATAAACGGC‑GTTGCTTTTCGGTGCGACGGCTAAATACACAATGGCTTGCGCAATAg < 1:491767/250‑1 (MQ=255) aaTTGGGTGTCTTTCAGTTGTGGCGGG‑AAGTAGTTTTCGCCGGCAGCGTAGGC‑GTTAGGTTCATTGTGGGCGTAGCGGTAGTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGA‑TTC‑GGTTG‑CC‑AATTTTTTGGC‑GG‑CTTT‑G‑AAAGCGAGATAAACGGC‑GTTGCTTTTCGGTGCGACGGCTAAATACACAATGGCTTGCGCAATAg < 1:438665/250‑1 (MQ=255) gtCTTTCAGTTGTGGCGGG‑AAGTAGTTTTCGCCGGCAGCGTAGGC‑GTTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGA‑TTC‑GGTTG‑CC‑AATTTTTTGGC‑GG‑CTTT‑G‑AAAGCGAGATAAACGGC‑GTTGCTTTTCGGTGCGACGGCTAAATACACAATgg < 1:401194/230‑1 (MQ=255) tCTTTCAGTTGTGGCGGG‑AAGTAGTTTTCGCCGGCAGCGTAGGC‑GTTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGA‑TTC‑GGTTG‑CC‑AATTTTTTGGC‑GG‑CTTT‑G‑AAAGCGAGATAAACGGC‑GTTGCTTTTCGGTGCGACGGCTAAATACACAATGGCTTGCGCAATAGCGCGTTCGc < 1:266856/250‑1 (MQ=255) cAGTTGTGGCGGG‑AAGTAGTTTTCGCCGGCAGCGTAGGC‑GTTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGA‑TTC‑GGTTG‑CC‑AATTTTTTGGC‑GG‑CTTT‑G‑AAAGCGAGATAAACGGC‑GTTGCTTTTCGGTGCGACGGCTAAATac < 1:90756/217‑1 (MQ=255) ttGTGGCGGG‑AAGTAGTTTTCGCCGGCAGCGTAGGC‑GTTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGA‑TTC‑GGTTG‑CC‑AATTTTTTGGC‑GG‑CTTT‑G‑AAAGCGAGATAAACGGC‑GTTGCTTTTCGGTGCGACGGCTAAATACACAATGGCTTGCGCAATAGCGCGTTCGCCTTCGGc < 1:528633/249‑1 (MQ=255) g‑AAGTAGTTTTCGCCGGCAGCGTAGGC‑GTTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGa > 1:1007/1‑116 (MQ=255) gtagtTTTCGCCGGCAGCGTAGGC‑GTTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGA‑TTC‑GGTTG‑CC‑AATTTTTTGGC‑GG‑CTTT‑G‑AAAGCGAGATAAACGGC‑GTTGCTTTTCGGTGCGACGGCTAAATACACAATGGCTTGCGCAATAGCGCGTTCGCCTTCGGCAGCGCCGACGCggg > 1:58061/1‑251 (MQ=255) gtTTTCGCCGGCAGCGTAGGC‑GTTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGA‑TTC‑GGTTG‑CC‑AATTTTTTGGC‑GG‑CTTT‑G‑AAAGCGAGATAAACGGC‑GTTGCTTTTCGGTGCGACGGCTAAATacac < 1:213361/201‑1 (MQ=255) | GACAAAATCCAAGGATTTTGAACGTTGGCTTTGCCAATGGTACGAAGTGCAAGCGCAGCGCAGTAAAAGTAGGTCGATGCCGAAACAAACCTATTTATATCGCTTCTTACCACTTTCCTGATCTAACCCCCGCAGCCATTCCAACTTCTCCTTAATCTTAATTTCCATGCCTCTGGTGGTCGGGAAATAGAATTGGGTGTCTTTCAGTTGTGGCGGG‑AAGTAGTTTTCGCCGGCAGCGTAGGC‑GTTAGGTTCATTGTGGGCGTAGCGGTATTCGGCGCCGAAGCCGAGGTCTTTCATGAAGCTGGTCGGGGCGTTGCGGAGATGTTCAGGGACGTCGAAATCGGC‑GGA‑TTC‑GGTTG‑CC‑AATTTTTTGGC‑GG‑CTTT‑G‑AAAGCGAGATAAACGGC‑GTTGCTTTTCGGTGCGACGGCTAAATACACAATGGCTTGCGCAATAGCGCGTTCGCCTTCGGCAGCGCCGACGCGGG > 1/476348‑476817 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |