| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | 1 | 1,636,160 | 0 | C | T | 38.3% | 84.8 / 31.7 | 81 | L208F (CTT→TTT) | unknown | Magnesium and cobalt efflux protein CorC |
| Reads supporting (aligned to +/- strand): ref base C (43/7); new base T (0/31); total (43/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.44e-16 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GAATTTAATTTGCTGACCATGATTCGCCCGACCATGATCGTGCCGGAAAGCAAACGAGTAGACAGAATGCTGAAGGATTTCCGTTCCGAGCGCTTCCACATGGCGATTGTGGTGGATGAATTC‑GGCGCGGTGTCGGGTTT‑GGTGACCATTGAAGATGTGTTGGAACAAATCGTCGGGGATATTGAAGACGAATTC‑GATGAAGAAGAAGTGGCGGATATT‑CGCCAGCTTTCCCGCCACACCTATGCCGTGCGGGCATTGACGGACATTGCAGATTTCAATCAACAATTCAATACCCAATACGCCGATGAAGAAGTGGACACCATCGGC‑GG‑TTTGGTGATGCAGGCATTCGGCTATTTGCCGAAACGCGGCG‑AAGAAATTACGCTGGATAACCTGCATTTTAAAGTTACCTCCGCCGACAGCCGCCGCATCATCCAACTCCGTGTCACCGTGCCGGACGAGCA > 1/1635935‑1636395 | gaaTTTAATTTGCTGACCATGATTCGCCCGACCATGATCGTGCCGGAAAGCAAACGAGTAGACAGAATGCTGAAGGATTTCCGTTCCGAGCGCTTCCACATGGCGATTGTGGTGGATGAATTC‑GGCGCGGTGTCGGGTTT‑GGTGACCATTGAAGATGTGTTGGAACAAATCGTCGGGGATATTGAAGACGAATTC‑GATGAAGAAGAAGTGGCGGATATT‑CGCCAGCTTTCCCGCCACACCTATGCCGTGCg > 1:200992/1‑251 (MQ=255)gaaTTTAATTTGCTGACCATGATTCGCCCGACCATGATCGTGCCGGAAAGCAAACGAGTAGACAGAATGCTGAAGGATTTCCGTTCCGAGCGCTTCCACATGGCGATTGTGGTGGATGAATTC‑GGCGCGGTGTCGGGTTT‑GGTGACCATTGAAGATGTGTTGGAACAAATCGTCGGGGATATTGAAGACGAATTC‑GATGAAGAAGAAGTGGCGGATATT‑CGCCAGCTTTCCCGCCACACCTATGCCGTGCg > 1:77363/1‑251 (MQ=255)gaaTTTAATTTGCTGACCATGATTCGCCCGACCATGATCGTGCCGGAAAGCAAACGAGTAGACAGAATGCTGAAGGATTTCCGTTCCGAGCGCTTCCACATGGCGATTGTGGTGGATGAATTC‑GGCGCGGTGTCGGGTTT‑GGTGACCATTGAAGATGTGTTGGAACAAATCGTCGGGGATATTGAAGACGAATTC‑GATGAAGAAGAAGTGGCGGATATT‑CGCCAGCTTTCCCGCCACACCTATGCCGTGCg > 1:222284/1‑251 (MQ=255) aatttaatttGCTGTCCATGATTCGCCCGACCATGATCGTGCCGGAAAGCAAACGAGTAGACAGAATGCTGAAGGATTTCCGTTCCGAGCGCTTCCACATGGCGATTGTGGTGGATGAATTC‑GGCGCGGTGTCGGGTTT‑GGTGACCATTGAAGATGTGTTGGAACAAATCGTCGGGGATATTGAAGACGAATTC‑GATGAAGAAGAAGTGGCGGATATT‑CGCCAGCTTTCCCGCCACACCTATGc > 1:361443/1‑244 (MQ=255) aatttaatttGCTGACCATGATTCGCCCGACCATGATCGTGCCGGAAAGCAAACGAGTAGACAGAATGCTGAAGGATTTCCGTTCCGAGCGCTTCCACATGGCGATTGTGGTGGATGAATTC‑GGCGCGGTGTCGGGTTT‑GGTGACCATTGAAGATGTGTTGGAACAAATCGTCGGGGATATTGAAGACGAATTC‑GATGAAGAAGAAGTGGCGGATATT‑CGCCAGCTTTCCCGCCACACCTATGc > 1:669454/1‑244 (MQ=255) tgcccATGATTCGCCCGACCATGATGGTGCGGGAAGGAAAGCGAGTAGACAGAATGCTAAAGGATTTCCGTCCCGAGCGCTTCCACATGGCGTTTGTGGTGGATGAATTC‑GGGGCGGTGTGGGTTTT‑GGTGACAATGGAAGATGTGTTGAAAAAAATGGCCGGGTATATGGAAGCCGATTGG‑GATGAAGAGGAAGGGGCGGATATT‑CGCCGGTTTTCCCGCCACACCTATGCCGTGCGGGCATTGACGGAc < 1:220754/248‑1 (MQ=255) tGATTCGCCCGACCATGATCGTGCCGGAAAGCAAACGAGTAGACAGAATGCTGAAGGATTTCCGTTCCGAGCGCTTCCACATGGCGATTGTGGTGGATGAATTC‑GGCGCGGTGTCGGGTTT‑GGTGACCATTGAAGATGTGTTGGAACAAATCGTCGGGGATATTGAAGACGAATTC‑GATGAAGAAGAAGTGGCGGATATT‑CGGCAGCTTTCCCGCCCCACCTATGCCGTGCGGGCATTGACGGACATTGCa > 1:222661/1‑251 (MQ=255) tGATTCGCCCGACCATGATCGTGCCGGAAAGCAAACGAGTAGACAGAATGCTGAAGGATTTCCGTTCCGAGCGCTTCCACATGGCGATTGTGGTGGATGAATTC‑GGCGCGGTGTCGGGTTT‑GGTGACCATTGAAGATGTGTTGGAACAAATCGTCGGGGATATTGAAGACGAATTC‑GATGAAGAAGAAGTGGCGGATATT‑CGCCAGCTTTCCCGCCACACCTATGc > 1:767219/1‑226 (MQ=255) tGATCCGCCCGACCATGATCGTGCCGGAAAGAAAACGAGTAGACAGAATGCTGAAGGATTTCCGTTCCGAGCGTTTCCACAGGGCGTTTGTGGTGGATGAATTC‑GGCGGGGTGTGGGGTTT‑GGTGACCATTGAAGATGTGTTGGAAAAAATCGTCGGGGATATTGAAGACGAATTC‑GATAAAGAAGAGGGGGCGGATATT‑CGCCAGTTTTCCCGCCACACCTATGc < 1:849009/226‑1 (MQ=255) tGATCCGCCCGACCAGGGTCGGGCGGAAAAGAAAACGAGTAGAGAGAATGCTGAAGGATTTCGGTTCCGGGCGCTTCCACAGGGCGATGGGGGGGGATGAATTC‑GGCGGGGTG‑GGGGGTT‑GGTGCCCATTGAAGTTGTGTTGGAAAAAATCGGGGGGGATTTGGAAGACGATTTC‑GATGAAGAAGAGGGGGGGGATATT‑CGCCAGTTTTCCCGCCACACCTATGCCGTGCGGGCATTGACGGACATTGCAg < 1:178618/251‑1 (MQ=255) ggATTCGCCCGCCCATGTTCGTGCCGAAAAGCAAACGAGTAGACAGATTGCTAAAGGTTTTCCTTTCCGGGCGTTTCCACAGGGCGTTTGTGGTGGATG‑ATTCGGGCGGGGTGT‑GGGTTTGGGTGCCCTTTGAAGTTGTTTTGGAAAAAATCGTGGGGGATATTGAAGACAAATTC‑AATGAAAAAGAAGTGGCGGATTTT‑CGCCATTTTCCCCGCCACACCTATGCCGTGCGGGCATTGa < 1:208891/239‑1 (MQ=255) aTTCCCCCGACCATGTTCGTGCAGGAAAGCAACCGAGTAGACAGAATGCTGAAGTTTTTCCTTTCGGAGCGTTTCCACAGGGCGATTGTGGTGGATGAATTC‑GGGGCGGTGTGGGGTTT‑GGTAACCATTGAATATGTGTGGGAACAAATCGTCGGGAATATAGAAGACGATTTG‑GATGAAAAAGAAGGGGCGAATATT‑CCCCGGTTTTCCCGCCACACCTATGCCGTGCGGGCATTGACGGACATTGCAg < 1:550408/250‑1 (MQ=255) cGACCAGGATCGTGCCGGAAAGCAAACGAGTAGACAGAAGGCTGAAGGATTTCCGTTCCGAGCGCTCCCACAGGGCGATTGTGGTGGATGAATGC‑GGCGCGGTGTGGGTTTT‑GGTGACCATTGAAGAGGTGTTGAAAAAAATCGTCGGGAATATTAAAGACGAATTC‑GATGAAAAAGAAGGGGCGGATATT‑CGCCAGTTTTCCCGCCACACCTATGCCGTGCGGGCATTGACGGACATTGCAGATTtcaat < 1:894274/251‑1 (MQ=255) cccATGATCGTGCCGGAAAGCAAACGAGTAGACAGAATGCTGAAGGATTTCCGTTCCGAGCGCTCCCACAGGGCGATTGGGGTGGATGAATTC‑GGCGCGGTGTCGGGTTT‑GGGGACCATTGAAGATGTGTTGGAACAAGCGGTCGGGGATATTGAAGACGAATTC‑GATAAAAAAGAAGTGGC‑GATTTTCCGCCAGCTTTCCCGCCACACCTATGCCGTGCGGGCATTGACGGACATTGCAGATTtc < 1:511018/245‑1 (MQ=255) cccATGATCGTGCCGAAAAGAAAACGAGTAGACAGAATGCTGAAGGATTTCCTTTCCGGGCGTTTCCACATGGCGTTTGTGGTGGATGAATTC‑GGCGCGGTGTGGGTTTT‑GGTGCCCATTGAAGATGTGTTGGAACAAATCGTGGGGGATATTGAAGACGAATTC‑GATGAAGAAGAGGTGGCGGATATT‑CGCCAGTTTTCCCGCCACACCTATGCCGTGCGGGCa < 1:875933/224‑1 (MQ=255) acaaTGATCGTGCCGGAAAGCAAACGAGTAGACAGAATGCTGAAGGATTTCCGTTCCGAGCGCTTCCACATGGCGATTGTGGTGGATGAATTC‑GGCGCGGTGTCGGGTTT‑GGTGACCATTGAAGATGTGTTGGAACAAATCGTCGGGGATATTGAAGACGAATTC‑GATGAAGAAGAAGTGGCGGATATT‑CGCCAGCTTTCCCGCCACACCTATGCCGTGCGGGCATTGACGGACATTGCAGATTtcaatca > 1:781047/4‑251 (MQ=255) aCCATGATCGTGCCGGAAAGCAAACGAGTAGACAGAATGCTGAAGGATTTCCGTTCCGAGCGCTTCCACATGGCGATTGTGGTGGATGAATTC‑GGCGCGGTGTCGGGTTT‑GGTGACCATTGAAGATGTGTTGGAACAAATCGTCGGGGATATTGAAGACGAATTC‑GATGAAGAAGAAGTGGCGGATATT‑CGCCAGCTTTCCCGCCACACCTATGc > 1:815556/1‑215 (MQ=255) aCCATGATCGTGCCGGAAAGCAAACGAGTAGACAGAATGCTGAAGGATTTCCGTTCCGAGCGCTTCCACATGGCGATTGTGGTGGATGAATTC‑GGCGCGGTGTCGGGTTT‑GGTGACCATTGAAGATGTGTTGGAACAAATCGTCGGGGATATTGAAGACGAATTC‑GATGAAGAAGAAGTGGCGGATATT‑CGCCAGCTTTCCCGCCACACCTATGCCGTGCGGGCATTGACGGACATTGCAg > 1:205455/1‑241 (MQ=255) aCCATGATCGTGCCGGAAAGCAAACGAGTAGACAGAATGCTGAAGGATTTCCGTTCCGAGCGCTTCCACATGGCGATTGTGGTGGATGAATTC‑GGCGCGGTGTCGGGTTT‑GGTGACCATTGAAGATGTGTTGGAACAAATCGTCGGGGATATTGAAGACGAATTC‑GATGAAGAAGAAGTGGCGGATATT‑CGCCAGCTTTCCCGCCACACCTATGCCGTGCGGGCATTGACGGACATTGCAGATTtcaatca > 1:395757/1‑251 (MQ=255) ccATGATCGTGCCGGAAAGCAAACGAGTAGACAGAATGCTGAAGGATTTCCGTTCCGAGCGCTTCCACATGGCGATTGTGGTGGATGAATTC‑GGCGCGGTGTCGGGTTT‑GGTGACCATTGAAGATGTGTTGGAACAAATCGTCGGGGATATTGAAGACGAATTC‑GATGAAGAAGAAGTGGCGGATATT‑CGCCAGCTTTCCCGCCACACCTATGCCGTGCGGGCATTGACGGACCTTGCAGATTtcaatcaa > 1:806924/1‑251 (MQ=255) tGATCGTGCCGGAAAGCAAACGAGTAGACAGAATGCTGAAGGATTTCCGTTCCGAGCGCTTCCACATGGCGATTGTGGTGGATGAATTC‑GGCGCGGTGTCGGGTTT‑GGTGACCATTGAAGATGTGTTGGAACAAATCGTCGGGGATATTGAAGACGAATTC‑GATGAAGAAGAAGTGGCGGATATT‑CGCCAGCTTTCCCGCCACACCTATGCCGTGCGGGCATTGACGGACATTGCAGATTTCAATcaacaa > 1:98714/1‑251 (MQ=255) ccgaaaaGCAAGCGAGTAGACAGATGGCTGAAGGATTTCCGTTCCGGGCGCTTCCACATGGCGATTGTGGTGGATGAATTC‑GGCGGGGTGTCGGGTTT‑GGTGACCATTGAAGATGTGTGGGAACAAATCGTGGGGGATATTGAAGACAAATTC‑GATGAAGAAGAAGTGGCGAATATT‑CGCCAGTTTCCCCGCCACACCTATGCCGTGCGGGCATTGACGGACATTGCAGATTTCAATCAACAATTCAATAc < 1:891280/247‑1 (MQ=255) ccGGAAAGCAAACGAGTAGACAAAATGCTGAAGGTTTTCCGTCCCGGGCGCTTCCACAGGGCGTTTGTGGGGGATAAATTC‑GGCGGGGTGGGGGGTTT‑GGTGACCATTGAAGTTGTGTTGAAAAAAATCGTGGGGGATATTGAAGACAAATTC‑GATGAAGAAGAAGGGGCGAATATT‑CGCCAGTTTTCCCGCCACACCTATGCCGTGCGGGCATTGACGGACATTGCAGATTTCAATCAACAATTCAATAc < 1:253820/251‑1 (MQ=255) gAAAGCAAACGAGTAGAAAGAATGCTGAAGGATTTCCGTTCCGAGCGCTTCCACAGGGCGATTGTGGTGGATGAATTC‑GGCGCGGTGTGGGGTTT‑GGTGACCATTGAAGATGTGTTGAAACAAATCGTCGGGAATATTGAAGACGAATTC‑GATGAAGAAGAGGTGGCGGATATT‑CGCCATTTTTCCCGCCACACCTATGCCGTGCGGGCATTGACGGACATTGCAGATTTCAATCAACAATTCAATAccc < 1:351683/250‑1 (MQ=255) aaaggaggaGACAGAATGGGGAAGGTTTTCCGTTCCGAGCGCTTCCACATGGCGATTGTGGGGGATGAATGG‑GGCGCGGTGTGGGGTTG‑GGTGACCATTGAAGATGTGTTGGAACAAAGCGTGGGGGATATGGAAGACGAATTC‑GATGAAGAAGAAGTGGCGGATATC‑CGCCAGTTTTCCCGCCACACCTATGCCGTGCGGGCATTGACGGACATTGCAGATTTCAATCAACAATTCAATACCCAATACGc < 1:858176/243‑1 (MQ=255) aaCGAGTAGACAGAATGCTGAAGGATTTCCGTTCCGAGCGCTTCCACATGGCGATTGTGGTGGATGAATTC‑GGCGCGGTGTCGGGTTT‑GGTGACCATTGAAGATGTGTTGGAACAAATCGTCGGGGATATTGAAGACGAATTC‑GATGAAGAAGAAGTGGCGGATATT‑CGCCAGCTTTCCCGCCACACCTATGCCGTGCGGGCATTGACgg > 1:177677/1‑210 (MQ=255) cgaatAGACAGAATGCTGAAGGATTTCCGTGCCGGGCGCTTCCACATGGCGATTGTGGTGGATGAATTG‑GGCGGGGTGTCGGGTTG‑GGTGACCATTGAAGATGTGTTGAAAAAAATCGTCGGGGATATTGAAGACGAATTC‑GATAAAGAAGAAGGGGCGGATATT‑CGCCAGTTTTCCCGCCACACCTATGCCGTGCGGGCATTGACGGACATTGCAGATTTCAATCAACAATTCAATAc < 1:834065/235‑1 (MQ=255) cGAGTAGACAGAATGCTGAAGGATTTCCGTTCCGAGCGCTTCCACATGGCGATTGTGGTGGATGAATTC‑GGCGCGGTGTCGGGTTT‑GGTGACCATTGAAGATGTGTTGGAACAAATCGTCGGGGATATTGAAGACGAATTC‑GATGAAGAAGAAGTGGCGGATATT‑CGCCAGCTTTCCCGCCACACCTATGc > 1:880559/1‑191 (MQ=255) cGAGTAGACAGAATGCTGAAGGATTTCCGTTCCGAGCGCTTCCACATGGCGATTGTGGTGGATGAATTC‑GGCGCGGTGTCGGGTTT‑GGTGACCATTGAAGATGTGTTGGAACAAATCGTCGGGGATATTGAAGACGAATTC‑GATGAAGAAGAAGTGGCGGATATT‑CGCCAGCTTTCCCGCCACACCTATGCCGTGCGGGCATTGACGGAc > 1:358785/1‑210 (MQ=255) aGTAGAAAGAATGCTGAAGGATTTCCGTCCCGAGCGCTTCCACATGGCGATTGTGGTGGATGAATTC‑GGCGCGGTGTGGGGTTT‑GGTGCCCATTGAAGATGTGTTGGAAAAAATCGTGGGGGATATTGAAGACGAATTC‑GATGAAGAAGAAGTGGGGGATATT‑CGCCAGTTTTCCCGCCACACCTATGc < 1:237747/189‑1 (MQ=255) aaGAATGCTGAAGGATTTCCGTTCCGAGCGCTTCCACATGGTGATTGTGGTGGATGAATTC‑GGCGGGGTGTGGGGTTT‑GGTGACCATTGAAGATGTGTTGGAAAAAATCGTCGGGGATATTGAAGACGAATTC‑GATGAAAAAGAAGTGGCGGATATT‑CGCCAGTTTTCCCGCCACACCTATGCCGTGCGGGCATTGACGGACATTGCAGATTTCAATCAACAATTCAATACCCAATACGCCGATGaagaag < 1:317777/250‑1 (MQ=255) agaaggcggAAGGGTTCCCGTTCCGAGCGCTTCCAAGTGGCGTTTGTGGGGGATGAATTG‑GGGGGGGTGTGGGGTTT‑GGTGCCCATGGAATTTGTTTTGGAACAAATCGTCGGGGATATGGAAGCCGAATTC‑GATGAAAAAGAGGGGGCTGATTTA‑CACAATCTTTCCCGCCACGCCTATGCCGTGCGGGCATTGACGGACATTGCAGATTTCAATCAACAATTCAATACCCAATACGCCGATGaagaag < 1:435332/242‑1 (MQ=255) gAATGCTGAAGGATTTCCGTTCCGAGCGCTTCCACATGGCGATTGTGGTGGATGAATTC‑GGCGCGGTGTCGGGTTT‑GGTGACCATTGAAGATGTGTTGGAACAAATCGTCGGGGATATTGAAGACGAATTC‑GATGAAGAAGAAGTGGCGGATATT‑CGCCAGCTTTCCCGCCACACCTATGCCGTGCGGGCATTGACGGACATTGc > 1:83979/1‑205 (MQ=255) aatgcggagggttttCCGTTCGGGGCGTTTCCCCATGGCGTTGGGGGTGGATGAATTG‑GGCGGGGTGT‑GGGTTTGGGTGCCCATTGAAGATGGGTTGGAACAAATCGTGGGGGATATGGAAGACAAATTG‑GATGAAGAAGAGGGGGCGAATATC‑CGCCATTTTTCCCGCCACACCTATGCCGTGCGGGCATTGACGGACATTGCAGATTTCAATCAACAATTCAATACCCAATACGCCGATGAAGAAGTgg < 1:558279/239‑1 (MQ=255) aaTGCTGAAGGATTCCCGTTCCGGGCGCTTCCACGGGGCGTTTGTGGTGGATGAATCG‑GGCGGGGGGTGGGTTTT‑GGGGACCATTAAAGATGTGTTGAAACAAATCGTGGGGGATATTGAAGACGAATTC‑AATAAAAAAGAAGGGGGGAATATC‑CGCCAGCTTCCCCGCCACACCTATGCCGTGCGGGCATTGACGGACATTGCAGATTTCAATCAACAATTCAATACCCAATACGCCGATggtgtccact < 1:746243/251‑10 (MQ=255) aTGCTGAAGGATTTCCGTTCCGAGCGCTTCCACATGGCGATTGTGGTGGATGAATTC‑GGCGCGGTGTCGGGTTT‑GGTGACCATTGAAGATGTGTTGGAACAAATCGTCGGGGATATTGAAGACGAATTC‑GATGAAGAAGAAGTGGCGGATATT‑CGCCAGCTTTCCCGCCACACCTATGCCGTGCGGGCATTGACGGACATTGCAGATTTCAATCAACAATTCAATAccc > 1:74348/1‑229 (MQ=255) tGCTGAAGGTTTTCCGTTCGGGGCGCTTCCACATGGCGATTGTGGTGGATGAATTC‑GGCGGGGTGTCGGGTTG‑GGTGACCATGGAAGATGTGTTGAAAAAAATCGTGGGGGATATTGAAGACGATTTC‑GATGAAGAAGAGGTGGCGGATATT‑CGCCAGTTTTCCCGCCACACCTATGCCGTGCGGGCATTGACGGACATTGCAGATTTCAATCAACAATTCAATACCCAATACGCCGATGAAGAAGTGGac < 1:27301/251‑1 (MQ=255) gcgtttCCACATGGGGATTGTGGTGGATGAATTC‑GGCGCGGTGTGGGGTTT‑GGTGACCATTGAAGAGGTGTTGGAACAAATCGTCGGGGATATTGAAGACGAATTC‑GATGAAGAAGAAGGGGGGGATATT‑CGCCAGCTTCCCCGCCACACCTATGCCGTGCGGGCATTGACGGACATTGCAGATTTCAATCAACAATTCAATACCCAATACGCCGATGAAGAAGTGGACACCATCGGC‑GG‑TTTGGTGATg < 1:359636/246‑1 (MQ=255) cgcTTCCACATGGCGATTGTGGTGGATGAATTC‑GGCGCGGTGTCGGGTTT‑GGTGACCATTGAAGATGTGTTGGAACAAATCGTCGGGGATATTGAAGACGAATTC‑GATGAAGAAGAAGTGGCGGATATT‑CGCCAGCTTTCCCGCCACACCTATGCCGTGCGGGCATTGACGGACATTGCAGATTTCAATCAACAATTCAATACCCAATACGCCGATGAAGAAGAGGACACCATCGGCGGG‑TTTgggaagc > 1:614924/1‑245 (MQ=255) cTTCCACATGGCGATTGTGGTGGATGAATTC‑GGCGCGGTGTCGGGTTT‑GGTGACCATTGAAGATGTGTTGGAACAAATCGTCGGGGATATTGAAGACGAATTC‑GATGAAGAAGAAGTGGCGGATATT‑CGCCAGCTTTCCCGCCACACCTATGCCGTGCGGGCATTGACGGACATTGCAGATTTCAATCAACAATTCAATACCCAATACGc > 1:61007/1‑210 (MQ=255) ttCCACATGGCGTTTGTGGTGGATGAATTC‑GGCGCGGTGTGGGGTTT‑GGTGACCATTGAAGATGTGTTGGAACAAATCGTCGGGGATATTGAAGACGAATTC‑GATGAAGAAGAAGTGGCGGATATT‑CGCCAGCTTTCCCGCCTCACCTATGCCGTGCGGGCATTGACGGACATTGCAGATTTCAATCAACAATTCAATACCCAATACGCCGATGAAGAAGTGGACACCATCGGC‑GG‑TTTGGTGATGCAGGc < 1:665284/251‑1 (MQ=255) tCCACATGGCGATTGTGGTGGATGATTTC‑GGCGCGGTGTGGGTTTT‑GGTGACCATTGAGGATTTGTTGGAAAAAATCGTGGGGGTTTTTGAAGACGATTTC‑GATGAAGAAGAAGTGGCGAATATC‑CGCCAGTTTTCCCGCCACACCTATGCCGTGCGGGCATTGACGGACATTGCAGATTTCAATCAACAATTCAATACCCAATACGCCGATGAAGAAGTg < 1:26636/221‑1 (MQ=255) cGATTGTGGTGGATGAATTC‑GGCGCGGTGTCGGGTTT‑GGTGACCATTGAAGATGTGTTGGAACAAATCGTCGGGGATATTGAAGACGAATTC‑GATGAAGAAGAAGTGGCGGATATT‑CGCCAGCTTTCCCGCCACACCTATTCCGTGCGGGCATTGACGGACATTGCAGATTTCAATCAACAATTCAATACCCAATACGCCGATGAAGAAGTGGACACCATCGGC‑GG‑GTTGGGGAAGCAgggacttggctat > 1:797398/1‑240 (MQ=255) tgtgGGGGATGATTGC‑GGCGGGGTGTGGGGTTG‑GGTGCCAATTGAAGATGTGTTGGAACAAATCGTCGGGGATATGGAAGACGAATTC‑GATGAAGAAGAGGTGGCGGATATT‑CGCCATTTTTCCCGCCACACCTATGCCGTGCGGGCATTGACGGACATTGCAGATTTCAATCAACAATTCAATACCCAATACGCCGATGAAGAAGTGGACACCATCGGC‑GG‑TTTGGTGATGCAGGCATTCGGCTATTTGc < 1:638591/251‑1 (MQ=255) gATGAATTC‑GGCGCGGTGTCGGGTTT‑GGTGACCATTGAAGATGTGTTGGAACAAATCGTCGGGGATATTGAAGACGAATTC‑GATGAAGAAGAAGTGGCGGATATT‑CGCCAGCTTTCCCGCCACACCTATGCCGTGCGGGCATTGACGGACATTGCAGATTTCAATCAACAATTCAATACCCAATACGCCGATGAAGAAGTGGACACCATCGGCGGG‑TTTGG‑GATGCAGGCaattggccaatttccggaaaa > 1:314253/1‑231 (MQ=255) gtgtCGGGTTT‑GGTGACCATTGAAGATGTGTTGGAACAAATCGTCGGGGATATTGAAGACGAATTC‑GATGAAGAAGAAGTGGCGGATATT‑CGCCAGCTTTCCCGCCACACCTATGCCGTGCGGGCATTGACGGACATTGCAGATTTCAATCAACAATTCAATACCCAATACGc > 1:39693/1‑173 (MQ=255) gtgtCGGGTTT‑GGTGACCATTGAAGATGTGTTGGAACAAATCGTCGGGGATATTGAAGACGAATTC‑GATGAAGAAGAAGTGGCGGATATT‑CGCCAGCTTTCCCGCCACACCTATGCCGTGCGGGCATTGACGGACATTGCAGATTTCAATCAACAATTCAATACCCAATACGCCGATGAAGAAGTGGACACCATCGGCGGG‑TTTGG‑GATGCCAGCCTTTGGCTATTTGCCGAAACGCGGCG‑GAGaaaata > 1:406371/1‑247 (MQ=255) gtgtCGGGTTT‑GGTGACCATTGAAGATGTGTTGGAACAAATCGTCGGGGATATTGAAGACGAATTC‑GATGAAGAAGAAGTGGCGGATATT‑CGCCAGCTTTCCCGCCACACCTATGCCGTGCGGGCATTGACGGACATTGCAGATTTCAATCAACAATTCAATACCCAATACGCCGATGAAGAAGTGGACACCATCGGC‑GG‑GTTGGTGATGCAGGCATTCGGCTATTTTCCGAAAAGCGGCG‑AAGaaaata > 1:799711/1‑247 (MQ=255) tggggTTG‑GGTGCCCATTGAAGATGTGTGGGAAAAAAGCGTGGGGGATATGGAAGACGAATGC‑GATAAAGAAGAAGGGGCGGATTTC‑CGCCAGTTTTCCCGCCACACCTATGCCGTGCGGGCATTGACGGACATTGCAGATTTCAATCAACAATTCAATACCCAATACGCCGATGAAGAAGTGGACACCATCGGC‑GG‑TTTGGTGATGCAGGCATTCGGCTATTTGCCGAAACGCGGCG‑AAGAAATTACGc < 1:74702/248‑1 (MQ=255) gtttt‑GGTGCCCATTGAAGATGTGTTGGAACAAATCGCGGGGGATATTGAAGACGAATTC‑AATAAAAAAAAGGTGGCGGATGTC‑CGCCAGTTTTCCCGCCACACCTATGCCGTGCGGGCATTGACGGACATTGCAGATTTCAATCAACAATTCAATACCCAATACGCCGATGaagaag < 1:164087/176‑1 (MQ=255) tt‑GGTGACCATTGAAGATGTGTTGGAACAAATCGTCGGGGATATTGAAGACGAATTC‑GATGAAGAAGAAGTGGCGGATATT‑CGCCAGCTTTCCCGCCACACCTATGCCGTGCGGGCATTGACGGACATTGCAGATTTCAATCAACAATTCAATACCCAATAc > 1:835135/1‑162 (MQ=255) gTGACAATTGAAGATGTGTTGGAACAAATCGTGGGGGATATGGAAGACGAATTC‑GATGAAGAAGAGGGGGGGGATATT‑CGCCAGTTTTCCCGCCACACCTATGCCGTGCGGGCATTGACGGACATTGCAGATTTCAATCAACAATTCAATACCCAATACGCCGATGAAGAAGTGGACACCATCGGC‑GG‑TTTGGTGATGCAGGCATTCGGCTATTTGCCGAAACGCGGCG‑AAGAAATTACGCTGGATAAcc < 1:123028/250‑1 (MQ=255) agaaggtgaaggatgggtgggAACAAATCGGGGGGGATATTGAAGACGATTTC‑GAGGAAGAAGAAGTGGCGGATATT‑CGCCAGTTTCCCCGCCACACCTATGCCGTGCGGGCATTGACGGACATTGCAGATTTCAATCAACAATTCAATACCCAATACGCCGATGAAGAAGTGGACACCATCGGC‑GG‑TTTGGTGATGCAGGCATTCGGCTATTTGCCGAAACGCGGCG‑AAGAAATTACGCTGGATAACCt < 1:315467/231‑1 (MQ=255) agaaagtagAAGATGTGTTGGAAAAAGGCTGCGGGGTTATTGAAGACAAATTC‑GAGGAAGAAGAGGGGGGGTATATC‑CGCCATTTTTCCCGCCACACCTATGCCGTGCGGGCATTGACGGACATTGCAGATTTCAATCAACAATTCAATACCCAATACGCCGATGAAGAAGTGGACACCATCGGC‑GG‑TTTGGTGATGCAGGCATTCGGCTATTTGCCGAAACGCGGCG‑AAGAAATTACGCTGGATAAcc < 1:204037/241‑1 (MQ=255) aCCATTGAAGATGTGTTGGAACAAATCGTCGGGGATATTGAAGACGAATTC‑GATGAAGAAGAAGTGGCGGATATT‑CGCCAGCTTTCCCGCCACACCTATGCCGTGCGGGCATTGACGGACATTGCAGATTTCAATCAACAATTCAATACCCAATACGCCGATGAAGAAGTGGACACCATCGGC‑GGTTTTGGGGA‑GCAAGGATTCGGCTATTTTCCCAAAAACGGCG‑GAGaaaataagccgggaaaactgca > 1:436212/1‑232 (MQ=255) ccATTGAAGATGTGTTGGAACAAATCGTCGGGGATATTGAAGACGAATTC‑GATGAAGAAGAAGTGGCGGATATT‑CGCCAGCTTTCCCGCCACACCTATGCCg > 1:158502/1‑102 (MQ=255) ccATTGAAGATGTGTTGGAACAAATCGTCGGGGATATTGAAGACGAATTC‑GATGAAGAAGAAGTGGCGGATATT‑CGCCAGCTTTCCCGCCACACCTATGCCGTGCGGGCATTGACGGACATTGCAGATTTCAATCAACAATTCAATACCCAATAc > 1:221251/1‑155 (MQ=255) ccATTGAAGATGTGTTGGAACAAATCGTCGGGGATATTGAAGACGAATTC‑GATGAAGAAGAAGTGGCGGATATT‑CGCCAGCTTTCCCGCCACACCTATGCCGTGCGGGCATTGACGGACATTGCAGATTTCAATCAACAATTCAATACCCAATACGCCGATGAAGAAGTGGACACCATCGGC‑GG‑GTTGGTGATGCAGGCATTCGGGGATTTGCCGCAAAGCGGCG‑GAGAAATTACGCTGGATAACCTGCAt > 1:303048/1‑251 (MQ=255) ccATTAAAGATGTGTTGAAACAAATCGTCGGGGATATTGAAGACGAATTC‑GATGAAGAAGAAGTGGGGGATATC‑CGCCAGTTTCCCCGCCACACCTATGCCGTGCGGGCATTGACGGACATTGCAGATTTCAATCAACAATTCAATACCCAATAc < 1:401212/155‑1 (MQ=255) ttGAAGATGTGTGGAAACAAATCGTGGGGGATATTGAAGACGATTTC‑GAGGAAGAAGAAGGGGCGGATTTT‑CGCCAGCTTTCCCGCCACACCTATGCCGTGCGGGCATTGACGGACATTGCAGATTTCAATCAACAATTCAATACCCAATACGCCGATGAAGAAGTGGACACCATCGGC‑GG‑TTTGGTGATGCAGGCATTCGGCTATTTGCCGAAACGCGGCG‑AAGAAATTACGCTGGATAAcc < 1:558957/243‑1 (MQ=255) gAAGATGTGTTGGAACAAATCGTCGGGGATATTGAAGACGAATTC‑GATGAAGAAGAAGTGGCGGATATT‑CGCCAGCTTTCCCGCCACACCTATGCCGTGCGGGCATTGACGGACATTGCAGATTTCAATCAACAATTCAATACCCAATACGCCGATGAAGAAGTGGACACCATCGGC‑GG‑TTTgggg > 1:704940/1‑184 (MQ=255) aaGATGTGTTGGAACAAATCGTCGGGGATATTGAAGACGAATTC‑GATGAAGAAGAAGTGGCGGATATT‑CGCCAGCTTTCCCGCCACACCTATGCCGTGCGGGCATTGACGGACATTGCAGATTTCAATCAACAATTCAATACCCAATACGCCGATGAAGAAGTGGACACCATCGGC‑GG‑TTTTGTGATGCAGGCATTCGGCCATTTTCCGAAAAGCGGCG‑AAGAAATTTCGCTGGGTAAACTGCAtttttaa > 1:501090/1‑248 (MQ=255) gtgtTGGAACAAATCGTCGGGGATATTGAAGACGAATTC‑GATGAAGAAGAAGTGGCGGATATT‑CGCCAGCTTTCCCGCCACACCTATGCCGTGCGGGCATTGACGGACATTGCAGATTTCAATCAACAATTCAATACCCAAt > 1:335384/1‑142 (MQ=255) gtgtTGGAACAAATCGTCGGGGATATTGAAGACGAATTC‑GATGAAGAAGAAGTGGCGGATATT‑CGCCAGCTTTCCCGCCACACCTATGCCGTGCGGGCATTGACGGACATTGCAGATTTCAATCAACAATTCAATACCCAATACGCCGATGAAGAAGTGGACACCATCGGC‑GG‑TTTTGTGATGCCGGCCTTCGGCTATTTTCCCGAACGCGGCG‑AAGAAAATAAGCTGGATAAACTGCCtttttaaatta > 1:804942/1‑243 (MQ=255) tgtagaaaCAAATCGGGGGGGATTATAAAGACGAATTCAGA‑GAAGAAGAGGGGGCGGATATT‑CCCCCTTTTTCCCGCCACACCTATGCCGTGCGGGCATTGACGGACATTGCAGATTTCAATCAACAATTCAATACCCAATACGCCGATGAAGAAGTGGACACCATCGGC‑GG‑TTTGGTGATGCAGGCATTCGGCTATTTGCCGAAACGCGGCG‑AAGAAATTACGCTGGATAACCTGCATTTTAAAGTTAcc < 1:501624/245‑1 (MQ=255) cAAATCGTCGGGGATATTGAAGACGAATTC‑GATGAAGAAGAAGTGGCGGATATT‑CGCCAGCTTTCCCGCCACACCTATGCCGTGCGGGCATTGACGGACATTGCAGATTTCAATCAACAATTCAATACCCAATACGCCGATGAAGAAGTGGACACCATCGGC‑GG‑TTTGGTGATGCAGGCATTTGGCCATTTTTCGGAACCCGGCG‑AAAAAATTAAGCTGGGTAAACCGCAtttttaaaataacccctct > 1:838704/1‑234 (MQ=255) aaTCGTCGGGGATATTGAAGACGAATTC‑GATGAAGAAGAAGTGGCGGATATT‑CGCCAGCTTTCCCGCCACACCTATGCCGTGCGGGCATTGACGGACATTGCAGATTTCAATCAACAATTCAATACCCAATACGCCGATGAAGAAGTGGACACCATCGGC‑GG‑TTTTGTGATGCAGGCAATCGGCTATTTGCCGAAAAGCGGCG‑AAGAAATTACGCTGGGTAAACCGCATTTTTAAATTAcccccccccaca > 1:25445/1‑241 (MQ=255) gggATATTGAAGACGAATTC‑GATGAAGAAGAAGGGGCGGATATT‑CGCCAGTTTTCCCGCCACACCTATGCCGTGCGGGCATTGACGGACATTGCAGATTTCAATCAACAATTCAATAccc < 1:888541/120‑1 (MQ=255) ggATATGGAAGACGAATTC‑GATAAAGAAGAAGTGGGGGATTTT‑GGCCGGTTTTCCCGCCACACCTATGCCGTGCGGGCATTGACGGACATTGCAGATTTCAATCAACAATTCAATACCCAATACGCCGATGAAGAAGTGGACACCATCGGC‑GG‑TTTGGTGATGCAGGCa < 1:515075/169‑1 (MQ=255) atTGAAGACGAATTC‑GATGAAGAAGAAGTGGCGGATATT‑CGCCAGCTTTCCCGCCACACCTATGCCGTGCGGGCATTGACGGACATTGCAGATTTCAATCAACAATTCAATACCCAATACGCCGATGAAGAAGTGGACACCATCGGC‑GG‑TTTTGGGATGCAGGCATTCGGCTATTTTCCGAAACGCGGCG‑AAGAAAATACGCTGGGTAACCTGCCTTTTTAAATTACCTCCGCCCACAGCCGCCGcctcat > 1:876070/1‑251 (MQ=255) gAAGACGAATTC‑GATGAAGAAGAAGTGGCGGATATT‑CGCCAGCTTTCCCGCCACACCTATGCCGTGCGGGCATTGACGGACATTGCAGATTTCAATCAACAATTCAATACCCAATACGCCGATGAAGAAGTGGACACCATCGGC‑GG‑TTTTGTGATGCAGGCATTCGGCTATTTTCCGAAACGCCGCG‑AAGAAAATACGCTGGGTAAACTGCATTTTTAAGTTACCTCCCCCGACAACCGCCGCCTCATcc > 1:693260/1‑250 (MQ=255) gaagacaaatgggATGAAAAAGAGGTGGCGGATATC‑CGCCGGCTTCCCCGCCACACCTATGCCGTGCGGGCATTGACGGACATTGCAGATTTCAATCAACAATTCAATACCCAATACGCCGATGAAGAAGTGGACACCATCGGC‑GG‑TTTGGTGATGCAGGCATTCGGCTATTTGCCGAAACGCGGCG‑AAGAAATTACGCTGGATAACCTGCATTTTAAAGTTACCTCCGCCGACAGCCGCCGCATCATcc < 1:621953/238‑1 (MQ=255) gacaaaTTC‑GAGGAAGAAGAGGGGGCGGATATC‑CGCCAGTTTTCCCGCCACACCTATGCCGTGCGGGCATTGACGGACATTGCAGATTTCAATCAACAATTCAATACCCAATACGCCGATGAAGAAGTGGACACCATCGGC‑GG‑TTTGGTGATGCAGGCATTCGGCTATTTGCCGAAACGCGGCG‑AAGAAAtt < 1:277793/188‑1 (MQ=255) gaagaagaaGTGGCGGATATT‑CGCCAGCTTTCCCGCCACACCTATGCCGTGCGGGCATTGACGGACATTGCAGATTTCAATCAACAATTCAATACCCAATACGCCGATGAAGAAGTGGACACCATCGGC‑GG‑TTTGGGGATGCAGGCATTTCGCCATTTTCCGGAAAGCGGCC‑AAGAAATTACGGTGGGTAACCCGCATTTTAAAATTACCTCCCCCGACAACCCCCCCATCATcccaccccctgttaccc > 1:675568/1‑235 (MQ=255) gaagaagaaGGGGAGTATATC‑CGCCAGTTTCCCCGCCACACCTATGCCGTGCGGGCATTGACGGACATTGCAGATTTCAATCAACAATTCAATACCCAATACGCCGATGAAGAAGTGGACACCATCGGC‑GG‑TTTGGTGATGCAGGCATTCGGCTATTTGCCGAAACGCGGCG‑AAGAAATTACGCTGGATAACCTGCATTTTAAAGTTACCTCCGCCGACAGCCGCCGCATCATCCAACTCCGTGTCACCg < 1:804086/250‑1 (MQ=255) aagaagaagTGGCGGATATT‑CGCCAGCTTTCCCGCCACACCTATGCCGTGCGGGCATTGACGGACATTGCAGATTTCAATCAACAATTCAATACCCAATACGCCGATGAAGAAGTGGACACCATCGGC‑GG‑GTTGGTGATGCAGGCATTCGGCTATTTGCCGAAACGCGGCG‑GAGAAATTACGCTGGATAAACTGCATTTTTAAATTACCTCCCCCGACAGCCGCCGCATCATCCAACTCCGTGGCACCggg > 1:480212/1‑249 (MQ=255) aagaagaagTGGCGAATATT‑CGCCAGTTTTCCCGCCACACCTATGCCGTGCGGGCATTGACGGACATTGCAGATTTCAATCAACAATTCAATACCCAATACGCCGATGAAGAAGTGGACACCATCGGC‑GG‑TTTGGTGATGCAGGCATTCGGCTATTTGCCGAAACGCGGCG‑AAGAAATTACGCTGGATAACCTGCATTTTAAAGTTAcc < 1:526606/209‑1 (MQ=255) gaagaagTGGCGGATATT‑CGCCAGCTTTCCCGCCACACCTATGCCGTGCGGGCATTGACGGACATTGCAGATTTCAATCAACAATTCAATACCCAATACGCCGATGAAGAAGTGGACACCATCGGC‑GG‑TTTGGGGGTGCCGGGATTCGGCCATTTGCCCAAACGCGGGG‑CAGAAAATAAGCTGGGTAACCTGCCTTTTAAAATTACCTCCCCCGACAGCCCCCGCATCATcccacccccggttaccctgc > 1:101651/1‑232 (MQ=255) gaagTGGCGGATATT‑CGCCAGCTTTCCCGCCACACCTATGCCGTGCGGGCATTGACGGACATTGCAGATTTCAATCAACAATTCAATACCCAATACGCCGATGAAGAAGTGGACACCATCGGC‑GG‑TTTTGGGATGCAGGCATTTGGCTATTTTCCGCAAAGCCGCGCAAG‑AATTAAGCCGGGTAACCCGCATTTTTAAATTACCTCCGCCGACACCCGCCGCCTCCTCCCACTCCCTGTCCCCCTGcccgg > 1:524055/1‑248 (MQ=255) gTGGCGGATATT‑CGCCAGCTTTCCCGCCACACCTATGCCGTGCGGGCATTGACGGACATTGCAGATTTCAATCAACAATTCAATACCCAATACGCCGATGAAGAAGTGGACACCATCGGC‑GG‑TTTGGTGATGCAGGCATTCGGCTATTTGCCGAAACGCGGCG‑AAGAAATTACGCTGGATaaact > 1:654537/1‑182 (MQ=255) ggattttccGCCAGTTTCCCCGCCACACCTATGCCGTGCGGGCATTGACGGACATTGCAGATTTCAATCAACAATTCAATACCCAATACGCCGATGAAGAAGTGGACACCATCGGC‑GG‑TTTGGTGATGCAGGCATTCGGCTATTTGCCGAAACGCGGCG‑AAGAAATTACGCTGGATAACCTGCATTTTAAAGTTACCTCCGCCGACAGCCGCCGCATCATCCAACTCCGTGTCACCGTGCCGGACGAGCa < 1:211639/242‑1 (MQ=255) | GAATTTAATTTGCTGACCATGATTCGCCCGACCATGATCGTGCCGGAAAGCAAACGAGTAGACAGAATGCTGAAGGATTTCCGTTCCGAGCGCTTCCACATGGCGATTGTGGTGGATGAATTC‑GGCGCGGTGTCGGGTTT‑GGTGACCATTGAAGATGTGTTGGAACAAATCGTCGGGGATATTGAAGACGAATTC‑GATGAAGAAGAAGTGGCGGATATT‑CGCCAGCTTTCCCGCCACACCTATGCCGTGCGGGCATTGACGGACATTGCAGATTTCAATCAACAATTCAATACCCAATACGCCGATGAAGAAGTGGACACCATCGGC‑GG‑TTTGGTGATGCAGGCATTCGGCTATTTGCCGAAACGCGGCG‑AAGAAATTACGCTGGATAACCTGCATTTTAAAGTTACCTCCGCCGACAGCCGCCGCATCATCCAACTCCGTGTCACCGTGCCGGACGAGCA > 1/1635935‑1636395 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |