New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	1	= 1553645	115 (1.310)	5 (0.060)	5/418	6.2	4.4%	intergenic (+260/‑4)	unknown/unknown	Carbon starvation protein A/Transcriptional regulator MraZ
	?	1	= 1553779	106 (1.240)					coding (131/459 nt)	unknown	Transcriptional regulator MraZ
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

ATTGGCAGGGTGTTTTTTTATATCTGTGATTTCGGCTTAAAAAGATCCTGGCTTAAAAAACTTTACGTAGAAGATCCTGAAACAGCTTGCAACTTGAGCAAACAAATAATAAACTTAGCGAAAAGTGGTGGAAAGTGGTTTTTTGTGGAGAATTTTGGAAATAAATCCCAAATTCTGACCATCCAAACGGAAAAAAGGAATAAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  1/1553442‑1553645 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑aataaaCAAGGTTGACGAATATCCACGGTGCAAACCATTTGCCCTTGATTTTGTTCCAAAAGTTCGGCGCGATGGCGGGTCGGGATAGAAATTCTACCCTTCGCATCTAAATTAACCGCCGAAGCTCCACGAAACATGAATTTATTCCTTTTTTCCGTTTGGATGGTCAGAATTTGGGATTTATTTCCAAAATTCTCCACAAAAAACCACTTTCCACCACTTTTCGCTAAGTTTATTATTTGTTTGCTCAAGTT  <  1/1553779‑1553532                                                                                                                                                                                                                                                                                                                                                                                                                                                                      ATTGGCAGGGTGTTTTTTTATATCTGTGATTTCGGCTTAAAAAGATCCTGGCTTAAAAAACTTTACGTAGAAGATCCTGAAACAGCTTGCAACTTGAGCAAACAAATAATAAACTTAGCGAAAAGTGGTGGAAAGTGGTTTTTTGTGGAGAATTTTGGAAATAAATCCCAAATTCTGACCATCCAAACGGAAAAAAGGAATAAACAAGGTTGACGAATATCCACGGTGCAAACCATTTGCCCTTGATTTTG                                                                                                                                                                                                           <  1:313299/251‑1           TGTTTTTTTATATCTGTGATTTCGGCTTAAAAAGATCCTGGCTTAAAAAACTTTACGTAGAAGATCCTGAAACAGCTTGCAACTTGAGCAAACAAATAATAAACTTAGCGAAAAGTGGTGGAAAGTGGTTTTTTGTGGAGAATTTTGGAAATAAATCCCAAATTCTGACCATCCAAACGGAAAAAAGGAATAAACAAGGTTGACGAATATCCACGGTGCAAACCATTTGCCCTTGATTTTGTTCCAAAAG                                                                                                                                                                                                  <  1:329165/250‑1           TGTTTTTTTATATCTGTGATTTCGGCTTAAAAAGATCCTGGCTTAAAAAACTTTACGTAGAAGATCCTGAAACAGCTTGCAACTTGAGCAAACAAATAATAAACTTAGAGAAAAGTGGTGGGAAGTGGTTTTTTGTGGAGAATTTTGGAAATAAATCCCAAATTCTGACCATCCAAACGGAAAAAAGGAATAAACAAGGTTGACGAATATCCACGGTGCAAACCATTTGCCCTTGATTTTGTTCCAAAAGT                                                                                                                                                                                                 <  1:137953/251‑1                                                                                          CAACTTGAGCAAACAAATAATAAACTTAGCGAAAAGTGGTGGAAAGTGGTTTTTTGTGGAGAATTTTGGAAATAAATCCCAAATTCTGACCATCCAAACGGAAAAAAGGAATAAACAAGGTTGACGAATATCCACGGTGCAAACCATTTGCCCTTGATTTTGTTCCAAAAGTTCGGCGCGATGGCGGGTCGGGATAGAAATTCTACCCTTCGCATCTAAATTAACCGCCGAAGCTCCACGAAACATGAATT                                                                                                                  <  1:601448/251‑1                                                                                                                             GTGGTGGAAAGTGGTTTTTTGTGGAGAATTTTGGAAATAAATCCCAAATTCTGACCATCCAAACGGAAAAAAGGAATAAACAAGGTTGACGAATATCCACGGTGCAAACCATTTGCCCTTGATTTTGTTCCAAAAGTTCGGCGCGATGGCGGGTCGGGATAGAAATTCTACCCTTCGCATCTAAATTAACCGCCGAAGCTCCACGAAACCTGAATT                                                                                                                  >  1:770057/1‑216                                                                                                                                                                                                          AAACAAGGTTGACGAATATCCACGGTGCAAACCATTTGCCCTTGATTTTGTTCCAAAAGTTCGGCGCGATGGCGGGTCGGGATAGAAATTCTACCCTTCGCATCTAAATTAACCGCCGAAGCTCCACGAAACATGAATTTATTCCTTTTTTCCGTTTGGATGGTCAGAATTTGGGATTTATTTCCAAAATTCTCCACAAAAAACCACTTTCCACCACTTTTCGCTAAGTTTATTATTTGTTTGCTCAAGTT  >  1:504568/1‑251                                                                                                                                                                                                          AAACAAGGTTGACGAATATCCACGGTGCAAACCATTTGCCCTTGATTTTGTTCCAAAAGTTCGGCGCGATGGCGGGTCGGGATAGAAATTCTACCCTTCGCATCTAAATTAACCGCCGAAGCTCCACGAAACATGAATTTATTCCTTTTTTCCGTTTGGATGGTCAGAATTTGGGATTTATTTCCAAAATTCTCCACAAAAAACCACTTTCCACCACTTTTCGCTAAGTTTATTATTTG              >  1:44495/1‑239                                                                                                                                                                                                          AAACAAGGTTGACGAATATCCACGGTGCAAACCATTTGCCCTTGATTTTGTTCCAAAAGTTCGGCGCGATGGCGGGTCGGGATAGAAATTCTACCCTTCGCATCTAAATTAACCGCCGAAGCTCCACGAAACATGAATTTATTCC                                                                                                            >  1:273534/1‑145                                                                                                                                                                                                          AAACAAGGTTGACGAATATCCACGGTGCAAACCATTTGCCCTTGATTTTGTTCCAAAAGTTCGGCGCGATGGCGGGTCGGGATAGAAATTCTACCCTTCGCATCTAAATTAACCGCCGAAGCTCCACGAAACATGAATTTAT                                                                                                               <  1:860618/142‑1                                                                                                                                                                                                                                                                                                                                                                                                                                                                      ATTGGCAGGGTGTTTTTTTATATCTGTGATTTCGGCTTAAAAAGATCCTGGCTTAAAAAACTTTACGTAGAAGATCCTGAAACAGCTTGCAACTTGAGCAAACAAATAATAAACTTAGCGAAAAGTGGTGGAAAGTGGTTTTTTGTGGAGAATTTTGGAAATAAATCCCAAATTCTGACCATCCAAACGGAAAAAAGGAATAAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  1/1553442‑1553645 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑aataaaCAAGGTTGACGAATATCCACGGTGCAAACCATTTGCCCTTGATTTTGTTCCAAAAGTTCGGCGCGATGGCGGGTCGGGATAGAAATTCTACCCTTCGCATCTAAATTAACCGCCGAAGCTCCACGAAACATGAATTTATTCCTTTTTTCCGTTTGGATGGTCAGAATTTGGGATTTATTTCCAAAATTCTCCACAAAAAACCACTTTCCACCACTTTTCGCTAAGTTTATTATTTGTTTGCTCAAGTT  <  1/1553779‑1553532

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.