Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP028101	875,381	A→G	K85E (AAA→GAA)	C7Y58_04335 →	autotransporter domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP028101	875,381	0	A	G	100.0%	193.5 / NA	60	K85E (AAA→GAA)	C7Y58_04335	autotransporter domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/31);  total (29/31)

TAGCAAATGGAGTACCAACAAGAGAAGAAATAGCGACATCAAGAGAAAATTTAAGAAACTCAGTAGGAAGTTTGCAATCTAAGATAGACGAAGCAAGAGCAGAAAACTCAAAAAGTTTAGCAGGCTTAAGATTAGAATTAATACAATTAATGGAACAAGGGGATCAAGTAGTAAAATCACCTTGGATGTCATGGCAATTTGGAATAAACTATATGTACAGTAAATGGAATGGTACATATAAAGGAAAAGGAGATAAGGCAGAAAAATATCCATTTGAAGGAATATTTACAAGAGATACAAATTTATTCAATAGATATATGCCTACAAATAGTGAAAATTATTCATTATTGCCT  >  CP028101/875271‑875623                                                                                                               |                                                                                                                                                                                                                                                   caGCAAATGGAGTACCAACAAGAGAAGAAATAGCGACATCAAGAGAAAATTTAAGAAACTCAGTAGGAAGTTTGCAATCTAAGATAGACGAAGCAAGAGCAGAAAACTCAGAAAGTTTAGCAGGCTTAAGATTAGAATTAATACAATTAATGGAACAAGGGGATCAAGTAGTAAAATCACCTTGGATGTCATGGCAATTTGGAATAAACTATATGTACAGTAAATGGAATGGTACATATaaaggaaaagga                                                                                                        >  1:121900/2‑251 (MQ=14) caGCAAATGGAGTACCAACAAGAGAAGAAATAGCGACATCAAGAGAAAATTTAAGAAACTCAGTAGGAAGTTTGCAATCTAAGATAGACGAAGCAAGAGCAGAAAACTCAGAAAGTTTAGCAGGCTTAAGATTAGAATTAATACAATTAATGGAACAAGGGGATCAAGTAGTAAAATCACCTTGGATGTCATGGCAATTTGGAATAAACTATATGTACAGTAAATGGAATGGTACATATaaaggaaaagga                                                                                                        >  1:298986/2‑251 (MQ=14)  aGCAAATGGAGTACCAATAAGAGAAGAAATAGCGACATCAAGAGAAAATTTAAGAAACTCAGTAGGAAGTTTGCAATCTAAGATAGACGAAGCAAGAGCAGAAAACTCAGAAAGTTTAGCAGGCTTAAGATTAGAATTAATACAATTAATGGAACAAGGGGATCAAGTAGTAAAATCACCTTGGATGTCATGGCAATTTGGAATAAACTATATGTACAGTAAATGGAATGGTACATATAAAGGAAAAGgag                                                                                                       >  1:99459/1‑251 (MQ=14)    cAAATGGAGTACCAACAAGAGAAGAAATAGCGACATCAAGAGAAAATTTAAGAAACTCAGTAGGAAGTTTGCAATCTAAGATAGACGAAGCAAGGGCAGAAAACTCAGAAAGTTTAGCAGGCTTAAGATTAGAATTAATACAATTAATGGAACAAGGGGATCAAGTAGTAAAATCACCTTGGATGTCATGGCAATTTGGAATAAACTATATGTACAGTAAATGGAATGGTACATATAAAGGAAAAGgag                                                                                                       <  1:413844/249‑1 (MQ=14)    cAAATGGAGTACCAACAAGAGAAGAAATAGCGACATCAAGAGAAAATTTAAGAAACTCAGTAGGAAGTTTGCAATCTAAGATAGACGAAGCAAGAGCAGAAAACTCAGAAAGTTTAGCAGGCTTAAGATTAGAATTAATACAATTAATGGAACAAGGGGATCAAGTAGTAAAATCACCTTGGATGTCATGGCAATTTGGAATAAACTATATGTACAGTAAATGGAATGGTACATATaaaggaaaagg                                                                                                         <  2:58267/247‑1 (MQ=14)          gAGTACCAACAAGAGAAGAAATAGCGACATCAAGAGAAAATTTAAGAAACTCAGTAGGAAGTTTGCAATCTAAGATAGACGAAGCAAGAGCAGAAAACTCAGAAAGTTTAGCAGGCTTAAGATTAGAATTAATACAATTAATGGAACAAGGGGATCAAGTAGTAAAATCACCTTGGATGTCATGGCAATTTGGAATAAACTATATGTACAGTAAATGGAATGGTACATATAAAGGAAAAGGAGTTAAGGCa                                                                                               >  1:826534/1‑251 (MQ=14)          gAGTACCAACAAGAGAAGAAATAGCGACATCAAGAGAAAATTTAAGAAACTCAGTAGGAAGTTTGCAATCTAAGATAGACGAAGCAAGAGCAGAAAACTCAGAAAGTTTAGCAGGCTTAAGATTAGAATTAATACAATTAATGGAACAAGGGGATCAAGTAGTAAAATCACCTTGGATGTCATGGCAATTTGGAATAAACTATATGTACAGTAAATGGAATGGTACATATAAAGGAAAAGGAGATAAGGc                                                                                                <  2:436376/250‑1 (MQ=14)              aCCAACAAGAGAAGAAATAGCGACATCAAGAGAAAATTTAAGAAACTCAGTAGGAAGTTTGCAATCTAAGATAGACGAAGCAAGAGCAGAAAACTCAGAAAGTTTAGCAGGCTTAAGATTAGAATTAATACAATTAATGGAACAAGGGGATCAAGTAGTAAAATCACCTTGGATGTCATGGCAATTTGGAATAAACTATATGTACAGTAAATGGAATGGTACATATAAAGGAAAAGGAGATAAGGCAGaaa                                                                                           >  1:217074/1‑251 (MQ=14)              aCCAACAAGAGAAGAAATAGCGACATCAAGAGAAAATTTAAGAAACTCAGTAGGAAGTTTGCAATCTAAGATAGACGAAGCAAGAGCAGAAAACTCAGAAAGTTTAGCAGGCTTAAGATTAGAATTAATACAATTAATGGAACAAGGGGATCAAGTAGTAAAATCACCTTGGATGTCATGGCAATTTGGAATAAACTATATGTACAGTAAATGGAATGGTACATATAAAGGAAAAGGAGATAAGGCAGaaa                                                                                           >  2:256189/1‑251 (MQ=14)               ccAACAAGAGAAGAAATAGCGACATCAAGAGAAAATTTAAGAAACTCAGTAGGAAGTTTGCAATCTAAGATAGACGAAGCAAGAGCAGAAAACTCAGAAAGTTTAGCAGGCTTAAGATTAGAATTAATACAATTAATGGAACAAGGGGATCAAGTAGTAAAATCACCTTGGATGTCATGGCAATTTGGAATAAACTATATGTACAGTAAATGGAATGGTACATATAAAGGAAAAGGAGATAAGGCAGaaaa                                                                                          <  2:404924/251‑1 (MQ=14)                caacaaGAGAAGAAATAGCGACATCAAGAGAAAATTTAAGAAACTCAGTAGGAAGTTTGCAATCTAAGATAGACGAAGCAAGAGCAGAAAACTCAGAAAGTTTAGCAGGCTTAAGATTAGAATTAATACAATTAATGGAACAAGGGGATCAAGTAGTAAAATCACCTTGGATGTCATGGCAATTTGGAATAAACTATATGTACAGTAAATGGAATGGTACATATAAAGGAAAAGGAGATAAGGCAGaaaaa                                                                                         <  1:257119/251‑1 (MQ=14)                      gagaAGAAATAGCGACATCAAGAGAAAATTTAAGAAACTCAGTAGGAAGTTTGCAATCTAAGATAGACGAAGCAAGAGCAGAAAACTCAGAAAGTTTAGCAGGCTTAAGATTAGAATTAATACAATTAATGGAACAAGGGGATCAAGTAGTAAAATCACCTTGGATGTCATGGCAGTTTGGAATAAACTATATGTACAGTAAATGGAATGGTACATATAAAGGAAAAGGAGATAAGGCAGAAAAATATCCa                                                                                   >  1:667851/1‑251 (MQ=14)                              aTAGCGACATCAAGAGAAAATTTAAGAAACTCAGTAGGAAGTTTGCAATCTAAGATAGACGAAGCAAGAGCAGAAAACTCAGAAAGTTTAGCAGGCTTAAGATTAGAATTAATACAATTAATGGAACAAGGGGATCAAGTAGTAAAATCACCTTGGATGTCATGGCAATTTGGAATAAACTATATGTACAGTAAATGGAATGGTACATATAAAGGAAAAGGAGATAAGGCAGAAAAATATCCATTTGAAgg                                                                           <  1:555427/251‑1 (MQ=14)                              aTAGCGACATCAAGAGAAAATTTAAGAAACTCAGTAGGAAGTTTGCAATCTAAGATAGACGAAGCAAGAGCAGAAAACTCAGAAAGTTTAGCAGGCTTAAGATTAGAATTAATACAATTAATGGAACAAGGGGATCAAGTAGTAAAATCACCTTGGATGTCATGGCAATTTGGAATAAACTATATGTACAGTAAATGGAATGGTACATATAAAGGAAAAGGAGATAAGGCAGAAAAATATCCATTTGAAgg                                                                           <  2:274194/251‑1 (MQ=14)                              aTAGCGACATCAAGAGAAAATTTAAGAAACTCAGTAGGAAGTTTGCAATCTAAGATAGACGAAGCAAGAGCAGAAAACTCAGAAAGTTTAGCAGGCTTAAGATTAGAATTAATACAATTAATGGAACAAGGGGATCAAGTAGTAAAATCACCTTGGATGTCATGGCAATTTGGAATAAACTATATGTACAGTAAATGGAATGGTACATATAAAGGAAAAGGAGATAAGGCAGAAAAATATCCATTTGAAgg                                                                           <  2:595672/251‑1 (MQ=14)                              aTAGCGACATCAAGAGAAAATTTAAGAAACTCAGTAGGAAGTTTGCAATCTAAGATAGACGAAGCAAGAGCAGAAAACTCAGAAAGTTTAGCAGGCTTAAGATTAGAATTAATACAATTAATGGAACAAGGGGATCAAGTAGTAAAATCACCTTGGATGTCATGGCAATTTGGAATAAACTATATGTACAGTAAATGGAATGGTACATATAAAGGAAAAGGAGATAAGGCAGAAAAATATCCATTTGAAga                                                                           <  2:500945/251‑2 (MQ=14)                                  cGACATCAAGAGAAAATTTAAGAAACTCAGTAGGAAGTTTGCAATCTAAGATAGACGAAGCAAGAGCAGAAAACTCAGAAAGTTTAGCAGGCTTAAGATTAGAATTAATACAATTAATGGAACAAGGGGATCAAGTAGTAAAATCACCTTGGATGTCATGGCAATTTGGAATAAACTATATGTACAGTAAATGGAATGGTACATATAAAGGAAAAGGAGATAAGGCAGAAAAATATCCATTTGAAGGAata                                                                       <  1:481106/251‑1 (MQ=18)                                    aCATCAAGAGAAAATTTAAGAAACTCAGTAGGAAGTTTGCAATCTAAGATAGACGAAGCAAGAGCAGAAAACTCAGAAAGTTTAGCAGGCTTAAGATTAGAATTAATACAATTAATGGAACAAGGGGATCAAGTAGTAAAATCACCTTGGATGTCATGGCAATTTGGAATAAACTATATGTACAGTAAATGGAATGGTACATATAAAGGAAAAGGAGATAAGGCAGAAAAATATCCAtt                                                                                 <  1:367581/239‑1 (MQ=14)                                    aCATCAAGAGAAAATTTAAGAAACTCAGTAGGAAGTTTGCAATCTAAGATAGACGAAGCAAGAGCAGAAAACTCAGAAAGTTTAGCAGGCTTAAGATTAGAATTAATACAATTAATGGAACAAGGGGATCAAGTAGTAAAATCACCTTGGATGTCATGGCAATTTGGAATAAACTATATGTACAGTAAATGGAATGGTACATATAAAGGAAAAGGAGATAAGGCAGAAAAATATCCAtt                                                                                 >  2:367581/1‑239 (MQ=14)                                     cATCAAGAGAAAATTTAAGAAACTCAGTAGGAAGTTTGCAATCTAAGATAGACGAAGCAAGAGCAGAAAACTCAGAAAGTTTAGCAGGCTTAAGATTAGAATTAATACAATTAATGGAACAAGGGGATCAAGTAGTAAAATCACCTTGGATGTCATGGCAATTTGGAATAAACTATATGTACAGTAAATGGAATGGTACATATAAAGGAAAAGGAGATAAGGCAGAAAAATATCCATTTGAAGGAATAttt                                                                    <  2:860293/251‑1 (MQ=18)                                       tCAAGAGAAAATTTAAGAAACTCAGTAGGAAGTTTGCAATCTAAGATAGACGAAGCAAGAGCAGAAAACTCAGAAAGTTTAGCAGGCTTAAGATTAGAATTAATACAATTAATGGAACAAGGGGATCAAGTAGTAAAATCACCTTGGATGTCATGGCAATTTGGAATAAACTATATGTACAGTAAATGGAATGGTACATATAAAGGAAAAGGAGATAAGGCAGAAAAATATCCATTTGAAGGAATATTTAc                                                                  <  1:538698/251‑1 (MQ=18)                                       tCAAGAGAAAATTTAAGAAACTCAGTAGGAAGTTTGCAATCTAAGATAGACGAAGCAAGAGCAGAAAACTCAGAAAGTTTAGCAGGCTTAAGATTAGAATTAATACAATTAATGGAACAAGGGGATCAAGTAGTAAAATCACCTTGGATGTCATGGCAATTTGGAATAAACTATATGTACAGTAAATGGAATGGTACATATAAAGGAAAAGGAGATAAGGCAGAAAAATATCCATTTGAAGGAATATTTAc                                                                  <  2:574254/251‑1 (MQ=18)                                         aaGAGAAAATTTAAAAAACTCAGTAGGAAGTTTGCAATCTA‑GATAGTCGAAGCAAGAGCAGAAAACTCAGAAAGTTTAGCAGGCTTAAGATTAGAATTAATACAATTAATGGAACCAGGGGATCAAGTAGTAAAATCACCTTGGATGTCATGGCAATTTGGAATAAACTATATGTACAGTAAATGGAATGGTACATATAAAGGAAAAGGAGATAAGGCAGAAAAATATCCATTTGAAGGAATATTTACAag                                                               <  1:820893/251‑1 (MQ=255)                                            agaAAATTTAAGAAACTCAGTAGGAAGTTTGCAATCTAAGATAGTCGAAGCAAGAGCAGAAAACTCAGAAAGTCTAGCATGCTTAAGATCAGAATTAATACAATTAATGGAACAAGGGGATAAAGTAGTAAAATCACCTTGGATGTCATGGCAATTTGGAATAAACTATATGTAAAGTAAATGGAATGGTACATATAAAGGAAAAGGAGATAAGGCAGAAAAATATCCATTTGAAGGAATATTTACAag                                                               <  1:707545/249‑1 (MQ=11)                                            agaAAATTTAAGAAACTCAGTAGGAAGTTTGCAATCTAAGATAGACGAAGCAAGAGCAGAAAACTCAGAAAGTTTAGCAGGCTTAAGATTAGAATTAATACAATTAATGGAACAAGGGGATCAAGTAGTAAAATCACCTTGGATGTCATGGCAATTTGGAATAAACTATATGTACAGTAAATGGAATGGTACATATAAAGGAAAAGGAGATAAGGCAGAAAAATATCCATTTGAAGGAATATTTACAag                                                               <  1:332798/249‑1 (MQ=18)                                                aaTTTAAGAAACTCAGTAGGAAGTTTGCAATCTAAGATAGACGAAGCAAGAGCAGAAAACTCAGAAAGTTTAGCAGGCTTAAGATTAGAATTAATACAATTAATGGAACAAGGGGATCAAGTAGTAAAATCACCTTGGATGTCATGGCAATTTGGAATAAACTATATGTACAGTAAATGGAATGGTACATATAAAGGAAAAGGAGATAAGGCAGAAAAATATCCATTTGAAGGAATATTTACAAGAGATa                                                          >  2:695699/1‑250 (MQ=25)                                                          aCTCAGTAGGAAGTTTGCAATCTAAGATAGACGAAGCAAGAGCAGAAAACTCAGAAAGTTTAGCAGGCTTAAGATTAGAATTAATACAATTAATGGAACAAGGGGATCAAGTAGTAAAATCACCTTGGATGTCATGGCAATTTGGAATAAACTATATGTACAGTAAATGGAATGGTACATATAAAGGAAAAGGAGATAAGGCAGAAAAATATCCATTTGAAGGAATATTTACAAGAGATACAAATTTAtt                                                >  2:746498/1‑250 (MQ=39)                                                          aCTCAGTAGGAAGTTTGCAATCTAAGATAGACGAAGCAAGAGCAGAAAACTCAGAAAGTTTAGCAGGCTTAAGATTAGAATTAATACAATTAATGGAACAAGGGGATCAAGTAGTAAAATCACCTTGGATGTCATGGCAATTTGGAATAAACTATATGTACAGTAAATGGAATGGTACATATAAAGGAAAAGGAGATAAGGCAGAAAAATATCCATTTGAAGGAATATTTACAAGAGATACAAATTTATTc                                               <  2:290026/251‑1 (MQ=39)                                                             cAGTAGGAAGTTTGCAATCTAAGATAGACGAAGCAAGAGCAGAAAACTCAGAAAGTTTAGCAGGCTTAAGATTAGAATTAATACAATTAATGGAACAAGGGGATCAAGTAGTAAAATCACCTTGGATGTCATGGCGATTTGGAATAAACTATATGTACAGTAAATGGAATGGTACATATAAAGGAAAAGGAGATAAGGCAGAAATATATCCATTTGAAGGAATATTTACAAGAGATACAAATTTATTCAAt                                            >  1:799212/1‑251 (MQ=255)                                                             cAGTAGGAAGTTTGCAATCTAAGATAGACGAAGCAAGAGCAGAAAACTCAGAAAGTTTAGCAGGCTTAAGATTAGAATTAATACAATTAATGGAACAAGGGGATCAAGTAGTAAAATCACCTTGGATGTCATGGCAATTTGGAATAAACTATATGTACAGTAAATGGAATGGTACATATAAAGGAAAAGGAGATAAGGCAGAAAAATATCCATTTGAAGGAATATTTACAAGAGATACAAATTTATTCAAt                                            >  2:780900/1‑251 (MQ=255)                                                             cAGTAGGAAGTTTGCAATCTAAGATAGACGAAGCAAGAGCAGAAAACTCAGAAAGTTTAGCAGGCTTAAGATTAGAATTAATACAATTAATGGAACAAGGGGATCAAGTAGTAAAATCACCTTGGATGTCATGGCAATTTGGAATAAACTATATGTACACTAAATGGAATGGTACATATAAAGGAAAAGGACATAAGGCAGAAAAATATCCAGTTGAAGGAATATTTACAAGAGATACAAATTTATTCAAt                                            >  1:676241/1‑251 (MQ=255)                                                               gTAGGAAGTTTGCAATCTAAGATAGACGAAGCAAGAGCAGAAAACTCAGAAAGTTTAGCAGGCTTAAGATTAGAATTAATACAATTAATGGAACAAGGGGATCAAGTAGTAAAATCACCTTGGATGTCATGGCAATTTGGAATAAACTATATGTACAGTAAATGGAATGGTACATATAAAGGAAAAGGAGATAAGGCAGAAAAATATCCATTTGAAGGAATATTTACAAGAGATACAAATTTATTCAATAg                                          >  1:560421/1‑251 (MQ=255)                                                               gTAGGAAGTTTGCAATCTAAGATAGACGAAGCAAGAGCAGAAAACTCAGAAAGTTTAGCAGGCTTAAGATTAGAATTAATACAATTAATGGAACAAGGGGATCAAGTAGTAAAATCACCTTGGATGTCATGGCAATTTGGAATAAACTATATGTACAGTAAATGGAATGGTACATATAAAGGAAAAGGAGATAAGGCAGAAAAATATCCATTTGAAGGAATATTTACAAGAGATACAAATTTATTCAATAg                                          >  2:372143/1‑251 (MQ=255)                                                                tAGGAAGTTTGCAATCTAAGATAGACGAAGCAAGAGCAGAAAACTCAGAAAGTTTAGCAGGCTTAAGATTAGAATTAATACAATTAATGGAACAAGGGGATCAAGTAGTAAAATCACCTTGGATGTCATGGCAATTTGGAATAAACTATATGTACAGTAAATGGAATGGTACATATAAAGGAAAAGGAGATAAGGCAGAAAAATATCCATTTGAAGGAATATTTACAAGAGATACAAATTTATTCAAt                                            <  1:93841/248‑1 (MQ=255)                                                                   gAAGTTTGCAATCTAAGATAGACGAAGCAAGAGCAGAAAACTCAGAAAGTTTAGCAGGCTTAAGATTAGAATTAATACAATTAATGGAACAAGGGGATCAAGTAGTAAAATCACCTTGGATGTCATGGCAATTTGGAATAAACTATATGTACAGTAAATGGAATGGTACATATAAAGGAAAAGGAGATAAGGCAGAAAAATATCCATTTGAAGGAATATTTACAAGAGATACAAATTTATTCAATAGata                                       <  1:238607/250‑1 (MQ=255)                                                                      gTTTGCAATCTAAGATAGACGAAGCAAGAGCAGAAAACTCAGAAAGTTTAGCAGGCTTAAGATTAGAATTAATACAATTAATGGAACAAGGGGATCAAGTAGTAAAATCACCTTGGATGTCATGGCAATTTGGAATAAACTATATGTACAGTAAATGGAATGGTACATATAAAGGAAAAGGAGATAAGGCAGAAAAATATCCATTTGAAGGAATATTTACAAGAGATACAAATTTATTCAATAGATATATg                                   >  1:579548/1‑251 (MQ=255)                                                                       tttGCAATCTAAGATAGACGAAGCAAGAGCAGAAAACTCAGAAAGTTTAGCAGGCTTAAGATTAGAATTAATACAATTAATGGAACAAGGGGATCAAGTAGTAAAATCACCTTGGATGTCATGGCAATTTGGAATAAACTATATGTACAGTAAATGGAATGGTACATATAAAGGAAAAGGAGATAAGGCAGAAAAATATCCATTTGAAGGAATATTTACAAGAGATACAAATTTATTCAATAGATATATGc                                  <  2:667851/251‑1 (MQ=255)                                                                        ttGCAATCTAAGATAGACGAAGCAAGAGCAGAAAACTCAGAAAGTTTAGCAGGCTTAAGATTAGAATTAATACAATTAATGGAACAAGGGGATCAAGTAGTAAAATCACCTTGGATGTCATGGCAATTTGGAATAAACTATATGTACAGTAAATGGAATGGTACATATAAAGGAAAAGGAGATAAGGCAGAAAAATATCCATTTGAAGGAATATTTACAAGAGATACAAATTTATTCAATAGATATATGcc                                 <  2:69691/251‑1 (MQ=255)                                                                            aaTCTAAGATAGACGAAGCAAGAGCAGAAAACTCAGAAAGTTTAGCAGGCTTAAGATTAGAATTAATACAATTAATGGAACAAGGGGATCAAGTAGTAAAATCACCTTGGATGTCATGGCAATTTGGAATAAACTATATGTACAGTAAATGGAATGGTACATATAAAGGAAAAGGAGATAAGGCAGAAAAATATCCATTTGAAGGAATATTTACAAGAGATACAAATTTATTCAATAGATATATGCCTACa                             <  2:236305/251‑1 (MQ=255)                                                                             aTCTAAGATAGACGAAGCAAGAGCAGAAAACTCAGAAAGTTTAGCAGGCTTAAGATTAGAATTAATACAATTAATGGAACAAGGGGATCAAGTAGTAAAATCACCTTGGATGTCATGGCAATTTGGAATAAACTATATGTACAGTAAATGGAATGGTACATATAAAGGAAAAGGAGATAAGGCAGAAAAATATCCATTTGAAGGAATATTTACAAGAGATACAAATTTATTCAATAg                                          >  2:257850/1‑237 (MQ=255)                                                                             aTCTAAGATAGACGAAGCAAGAGCAGAAAACTCAGAAAGTTTAGCAGGCTTAAGATTAGAATTAATACAATTAATGGAACAAGGGGATCAAGTAGTAAAATCACCTTGGATGTCATGGCAATTTGGAATAAACTATATGTACAGTAAATGGAATGGTACATATAAAGGAAAAGGAGATAAGGCAGAAAAATATCCATTTGAAGGAATATTTACAAGAGATACAAATTTATTCAATAg                                          <  1:257850/237‑1 (MQ=255)                                                                             aTCTAAGATAGACGAAGCAAGAGCAGAAAACTCAGAAAGTTTAGCAGGCTTAAGATTAGAATTAATACAATTAATGGAACAAGGGGATCAAGTAGTAAAATCACCTTGGATGTCATGGCAATTTGGAATAAACTATATGTACAGTAAATGGAATGGTACATATAAAGGAAAAGGAGATAAGGCAGAAAAATATCCATTTGAAGGAATATTTACAAGAGATACAAATTTATTCAATAGATATATGCCTACaa                            >  2:272728/1‑251 (MQ=255)                                                                             aTCTAAGATAGACGAAGCAAGAGCAGAAAACTCAGAAAGTTTAGCAGGCTTAAGATTAGAATTAATACAATTAATGGAACAAGGGGATCAAGTAGTAAAATCACCTTGGATGTCATGGCAATTTGGAATAAACTATATGTACAGTAAATGGAATGGTACATATAAAGGAAAAGGAGATAAGGCAGAAAAATATCCATTTGAAGGAATATTTACAAGAGATACAAATTTATTCAATAGATATATGCCTACaa                            >  1:861744/1‑251 (MQ=255)                                                                             aTCTAAGATAGACGAAGCAAGAGCAGAAAACTCAGAAAGTTTAGCAGGCTTAAGATTAGAATTAATACAATTAATGGAACAAGGGGATCAAGTAGTAAAATCACCTTGGATGTCATGGCAATTTGGAATAAACTATATGTACAGTAAATGGAATGGTACATATAAAGGAAAAGGAGATAAGGCAGAAAAATATCCATTTGAAGGAATATTTACAAGAGATACAAATTTATTCAATAGATATATGCCTACaa                            >  1:501399/1‑251 (MQ=255)                                                                             aTCTAAGATAGACGAAGCAAGAGCAGAAAACTCAGAAAGTTTAGCAGGCTTAAGATTAGAATTAATACAATTAATGGAACAAGGGGATCAAGTAGTAAAATCACCTTGGATGTCATGGCAATTTGGAATAAACTATATGTACAGTAAATGGAATGGTACATATAAAGGAAAAGGAGATAAGGCAGAAAAATATCCATTTGAAGGAATATTTACAAGAGATACAAATTTATTCAATAGATATATGCCTACaa                            >  2:179175/1‑251 (MQ=255)                                                                               cTAAGATAGACGAAGCAAGAGCAGAAAACTCAGAAAGTTTAGCAGGCTTAAGATTAGAATTAATACAATTAATGGAACAAGGGGATCAAGTAGTAAAATCACCTTGGATGTCATGGCAATTTGGAATAAACTATATGTACAGTAAATGGAATGGTACATATAAAGGAAAAGGAGATAAGGCAGAAAAATATCCATTTGAAGGAATATTTACAAGAGATACAAATTTATTCAATAGATATATGCCTACAAAt                          <  2:861744/251‑1 (MQ=255)                                                                               cTAAGATAGACGAAGCAAGAGCAGAAAACTCAGAAAGTTTAGCAGGCTTAAGATTAGAATTAATACAATTAATGGAACAAGGGGATCAAGTAGTAAAATCACCTTGGATGTCATGGCAATTTGGAATAAACTATATGTACAGTAAATGGAATGGTACATATAAAGGAAAAGGAGATAAGGCAGAAAAATATCCATTTGAAGGAATATTTACAAGAGATACAAATTTATTCAATAGATATATGCCTACAAAt                          <  1:256189/251‑1 (MQ=255)                                                                               cTAAGATAGACGAAGCAAGAGCAGAAAACTCAGAAAGTTTAGCAGGCTTAAGATTAGAATTAATACAATTAATGGAACAAGGGGATCAAGTAGTAAAATCACCTTGGATGTCATGGCAATTTGGAATAAACTATATGTACAGTAAATGGAATGGTACATATAAAGGAAAAGGAGATAAGGCAGAAAAATATCCATTTGAAGGAATATTTACAAGAGATACAAATTTATTCAATAGATATATGCCTACAAAt                          <  2:298986/251‑1 (MQ=255)                                                                                 aaGATAGACGAAGCAAGAGCAGAAAACTCAGAAAGTTTAGCAGGCTTAAGATTAGAATTAATACAATTAATGGAACAAGGGGATCAAGTAGTAAAATCACCTTGGATGTCATGGCAATTTGGAATAAACTATATGTACAGTAAATGGAATGGTACATATAAAGGAAAAGGAGATAAGGCAGAAAAATATCCATTTGAAgg                                                                           >  1:364799/1‑200 (MQ=17)                                                                                 aaGATAGACGAAGCAAGAGCAGAAAACTCAGAAAGTTTAGCAGGCTTAAGATTAGAATTAATACAATTAATGGAACAAGGGGATCAAGTAGTAAAATCACCTTGGATGTCATGGCAATTTGGAATAAACTATATGTACAGTAAATGGAATGGTACATATAAAGGAAAAGGAGATAAGGCAGAAAAATATCCATTTGAAGGAATATTTACAAGAGATACAAATTTATTCAATAGATATATGCCTACAAATAg                        >  1:35845/1‑251 (MQ=255)                                                                                 aaGATAGACGAAGCAAGAGCAGAAAACTCAGAAAGTTTAGCAGGCTTAAGATTAGAATTAATACAATTAATGGAACAAGGGGATCAAGTAGTAAAATCACCTTGGATGTCATGGCAATTTGGAATAAACTATATGTACAGTAAATGGAATGGTACATATAAAGGAAAAGGAGATAAGGCAGAAAAATATCCATTTGAAGGAATATTTACAAGAGATACAAATTTATTCAATAGATATATGCCTACAAATAg                        <  2:583643/251‑1 (MQ=255)                                                                                 aaGATAGACGAAGCAAGAGCAGAAAACTCAGAAAGTTTAGCAGGCTTAAGATTAGAATTAATACAATTAATGGAACAAGGGGATCAAGTAGTAAAATCACCTTGGATGTCATGGCAATTTGGAATAAACTATATGTACAGTAAATGGAATGGTACATATAAAGGAAAAGGAGATAAGGCAGAAAAATATCCATTTGAAGGAATATTTACAAGAGATACAAATTTATTCAATAGATATATGCCTACAAATAg                        >  1:4592/1‑251 (MQ=255)                                                                                 aaGATAGACGAAGCAAGAGCAGAAAACTCAGAAAGTGTAGCAGGCTTAAGATTAGAATTAATACAATTAAAGGAACAAGGGGATCAAGTAGTAAAATCACCTTGGATGTCATGGCAATTTGGAATAAACGATATGTACAGTAAATGGAATGGTACATATAAAGGAAAAGGAGATAAGGCAGAAAAATATCCATTTGAAgg                                                                           <  2:364799/200‑1 (MQ=255)                                                                                    aTAGACGAAGCAAGAGCAGAAAACTCAGAAAGTTTAGCAGGCTTAAGATTAGAATTAATACAATTAATGGAACAAGGGGATCAAGTAGTAAAATCACCTTGGATGTCATGGCAATTTGGAATAAACTATATGTACAGTAAATGGAATGGTACATATAAAGGAAAAGGAGATAAGGCAGAAAAATATCCATTTGAAGGAATATTTACAAGAGATACAAATTTATTCAATAGATATATGCCTACAAATAGTGa                     >  2:787695/1‑251 (MQ=255)                                                                                       gACGAAGCAAGAGCAGAAAACTCAGAAAGTTTAGCAGGCTTAAGATTAGAATTAATACAATTAATGGAACAAGGGGATCAAGTAGTAAAATCACCTTGGATGTCATGGCAATTTGGAATAAACTATATGTACAGTAAATGGAATGGTACATATAAAGGAAAAGGAGATAAGGCAGAAAAATATCCATTTGAAGGAATATTTACAAGAGATACAAATTTATTCAATAGATATATGCCTACAAATAGTGaaaa                  >  1:269059/1‑251 (MQ=255)                                                                                       gACGAAGCAAGAGCAGAAAACTCAGAAAGTTTAGCAGGCTTAAGATTAGAATTAATACAATTAATGGAACAAGGGGATCAAGTAGTAAAATCACCTTGGATGTCATGGCAATTTGGAATAAACTATATGTACAGTAAATGGAATGGTACATATAAAGGAAAAGGAGATAAGGCAGAAAAATATCCATTTGAAGGAATATTTACAAGAGATACAAATTTATTCAATAGATATATGCCTACAAATAGTGaaaa                  >  1:520340/1‑251 (MQ=255)                                                                                           aaGCAAGGGCAGAAAACTCAGAAAGTTTAGCAGGCTTAAGATTAGAATTAATACAATTAATGGAACAAGGGGATCAAGTAGTAAAATCACCTTGGATGTCATGGCAATTTGGAATAAACTATATGTACAGTAAATGGAATGGTACATATAAAGGAAAAGGAGATAAGGCAGAAAAATATCCATTTGAAGGAATATTTACAAGAGATACAAATTTATTCAATAGATATATGCCTACAAATAGTGAAAattat              >  1:890130/1‑251 (MQ=255)                                                                                            aGCAAGAGCAGAAAACTCAGAAAGTTTAGCAGGCTTAAGATTAGAATTAATACAATTAATGGAACAAGGGGATCAAGTAGTAAAATCACCTTGGATGTCATGGCAATTTGGAATAAACTATATGTACAGTAAATGGAATGGTACATATAAAGGAAAAGGAGATAAGGCAGAAAAATATCCATTTGAAGGAATATTTACAAGAGATACAAATTTATTCAATAGATATATGCCTACAAATAGTGAAAattatt             >  1:183631/1‑251 (MQ=255)                                                                                                       aaaaCTCAGAAAGTTTAGCAGGCTTAAGATTAGAATTAATACAATTAATGGAACAAGGGGATCAAGTAGTAAAATCACCTTGGATGTCATGGCAATTTGGAATAAACTATATGTACAGTAAATGGAATGGTACATATAAAGGAAAAGGAGATAAGGCAGAAAAATATCCATTTGAAGGAATATTTACAAGAGATACAAATTTATTCAATAGATATATGCCTACAAATAGTGAAAATTATTCATTATTGcc   <  2:121900/250‑1 (MQ=255)                                                                                                       aaaaCTCAGAAAGTTTAGCAGGCTTAAGATTAGAATTAATACAATTAATGGAACAAGGGGATCAAGTAGTAAAATCACCTTGGATGTCATGGCAATTTGGAATAAACTATATGTACAGTAAATGGAATGGTACATATAAAGGAAAAGGAGATAAGGCAGAAAAATATCCATTTGAAGGAATATTTACAAGAGATACAAATTTATTCAATAGATATATGCCTACAAATAGTGAAAATTATTCATTATTGCCt  <  2:890130/251‑1 (MQ=255)                                                                                                               |                                                                                                                                                                                                                                                   TAGCAAATGGAGTACCAACAAGAGAAGAAATAGCGACATCAAGAGAAAATTTAAGAAACTCAGTAGGAAGTTTGCAATCTAAGATAGACGAAGCAAGAGCAGAAAACTCAAAAAGTTTAGCAGGCTTAAGATTAGAATTAATACAATTAATGGAACAAGGGGATCAAGTAGTAAAATCACCTTGGATGTCATGGCAATTTGGAATAAACTATATGTACAGTAAATGGAATGGTACATATAAAGGAAAAGGAGATAAGGCAGAAAAATATCCATTTGAAGGAATATTTACAAGAGATACAAATTTATTCAATAGATATATGCCTACAAATAGTGAAAATTATTCATTATTGCCT  >  CP028101/875271‑875623

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑