Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP028101	761,062	A→G	K479K (AAA→AAG)	C7Y58_03755 →	hemolysin activator protein precursor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP028101	761,062	0	A	G	100.0%	120.5 / NA	33	K479K (AAA→AAG)	C7Y58_03755	hemolysin activator protein precursor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/14);  total (19/14)

CAATAGGTGGAGTAGGAAGTGTTGGTGGTTATCATAGAACTGGAAATATACAAGGTGATAAAGCAATAGAAATAGAAAATGAATTATCATATAGAGTGTTAGACTCAGAAAAATTTGGAAAAATAACTCCTTATTTAAGTTATTCATATGGAAAAGTAAGAAATAACAAAAATAATTCAAAATATAGAAAAGGATATATGTCAGGTGCTATATTAGGTTTAAGATATAATATGAAATATTTAGATTTAGATGTAGCTTATGCAAAACCTTTAGCTCGTTCAAATTACTTAAAACCTAAGAATAGAGAAATATATTTTAGTGCAACATTAAAAATTAAATTTTAGATGGGAGGGAAT  >  CP028101/760950‑761305                                                                                                                 |                                                                                                                                                                                                                                                    cAATAGGTGGAGTAGGAAGTGTTGGTGGTTATCATAGAACTGGAAATATACAAGGTGATAAAGCAATGGAAATAGAAAATGAATTATCATATAGAGTGTTAGACTCAGAAAAGTTTGGAAAAATAACTCCTTATTTAAGTTATTCATATGGAAAAGTAAGAAATAACAAAAATAATTCAAAATATAGAAAAGGATATATGTCAGGTGCTATATTAGGTTTAAGATATAATATGAAATATttagatttagat                                                                                                           <  2:149747/251‑1 (MQ=18)  aaTAGGTGGAGTAGGAAGTGTTGGTGGTTATCATAGAACTGGAAATATACAAGGTGATAAAGCAATGGAAATAGAAAATGACTTATCATATAGAGTGTTAGACTCAGAAAAGTTTGGAAAAATAACTCCTTATTTAAGTTATTCATATGGAAAAGTAAGAAATAACAAAAATAATTCAAAATATAGAAAAGGATATATGTCAGGTGCTATATTAGGTTTAAGATATAATATGAAATATTTAGATTTAGATg                                                                                                          <  1:787873/251‑1 (MQ=21)       gTGGAGTAGGAAGTGTTGGTGGTTATCATAGAACTGGAAATATACAAGGTGATAAAGCAATGGAAATAGAAAATGAATTATCATATAGAGTGTTAGACTCAGAAAAGTTTGGAAAAATAACTCCTTATTTAAGTTATTCATATGGAAAAGTAAGAAATAACAAAAATAATTCAAAATATAGAAAAGGATATATGTCAGGTGCTATATTAGGTTTAAGATATAATATGAAATATTTAGATTTAGATGTAg                                                                                                       >  2:800410/1‑249 (MQ=25)        tGGAGTTGGAAGTGTTGGTGGTTATCATAGAACTGGAAATATACAAGGTGATAAAGCAATGGAAATAGAAAATGAATTATCATATAGAGTGTTAGACTCAGAAAAGTTTGGAAACATAACTCCTTATTTAAGTTATTCATATGGAAAAGTAAGAAATAACAAAAATAATTCAAAATATAGAAAAGGATATATGTCAGGTGCTATATTAGGTTTAAGATATAATATGAAATATTTAGATTTAGATGTAGCt                                                                                                     <  1:834216/250‑1 (MQ=255)        tGGAGTAGGAAGTGTTGGTGGTTATCATAGAACTGGAAATATACAAGGTGATAAAGCAATGGAAATAGAAAATGAATTATCATATAGAGTGTTAGACTCAGAAAAGTTTGGAAAAATAACTCCTTATTTAAGTTATTCATATGGAAAAGTAAGAAATAACAAAAATAATTCAAAATATAGAAAAGGATATATGTCAGGTGCTATATTAGGTTTAAGATATAATATGAAATATTTAGATTTAGATGTAGCt                                                                                                     <  2:892798/250‑1 (MQ=37)          gAGTAGGAAGTGTTGGTGGTTATCATAGAACTGGAAATATACAAGGTGATAAAGCAATGGAAATAGAAAATGAATTATCATATAGAGTGTTAGACTCAGAAAAGTTTGGAAAAATAACTCCTTATTTAAGTTATTCATATGGAAAAGTAAGAAATAACAAAAATAATTCAAAATATAGAAAAGGATATATGTCAGGTGCTATATTAGGTTTAAGATATAATATGAAATATTTAGATTTAGATGTAGCTTAt                                                                                                  >  2:898957/1‑251 (MQ=255)           aGTAGGAAGTGTTGGTGGTTATCATAGAACTGGAAATATACAAGGTGATAAAGCAATGGAAATAGAAAATGAATTATCATATAGAGTGTTAGACTCAGAAAAGTTTGGAAAAATAACTCCTTATTTAAGTTATTCATATGGAAAAGTAAGAAATAACAAAAATAATTCAAAATATAGAAAAGGATATATGTCAGGTGCTATATTAGGTTTAAGATATAATATGAAATATTTAGATTTAGATGTAGCTTATg                                                                                                 >  2:676437/1‑251 (MQ=255)             tAGGAAGTGTTGGTGGTTATCATAGAACTGGAAATATGCAAGGTGATAAAGCAATGGACATAGAAAATGAATTAGCATATCGAGTGTTAGACTCAGAAAAGTTTGGAAAAATAACTCCTTATTTAAGTTATTCATATGGAAAAGTAAGAAATAACAAAAATAATTCAAAATATAGAAAAGGATATATGTCAGGTGCTATATTAGGTTTAAGATATAATATGAAATATTTAGATTTAGATGTAGCTTATGc                                                                                                <  1:676437/250‑1 (MQ=255)                gAAGTGTTGGTGGTTATCATAGAACTGGAAATATACAAGGTGATAAAGCAATGGAAATAGAAAATGAATTATCATATAGAGTGTTAGACTCAGAAAAGTTTGGAAAAATAACTCCTTATTTAAGTTATTCATATGGAAAAGTAAGAAATAACAAAAATAATTCAAAATATAGAAAAGGATATATGTCAGGTGCTATATTAGGTTTAAGATATAATATGAAATATTTAGATTTAGATGTAGCTTATGCaaaa                                                                                            >  1:384413/1‑251 (MQ=255)                   gtgtTGGTGGTTATCATAGAACTGGAAATATACAAGGTGATAAAGCAATGGAAATAGAAAATGAATTATCATATAGAGTGTTAGACTCAGAAAAGTTTGGAAAAATAACTCCTTATTTAAGTTATTCATATGGAAAAGTAAGAAATAACAAAAATAATTCAAAATATAGAAAAGGATATATGTCAGGGGCTATATTAGGATTAAGATATAATATGAAATATTTAGATTTAGATGTAGCTTATGCAAAAc                                                                                           >  1:269574/1‑249 (MQ=255)                     gtTGGTGGTTATCATAGAACTGGAAATATACAAGGTGATAAAGCAATGGAAATAGAAAATGAATTATCATATAGAGTGTTAGACTCAGAAAAGTTTGGAAAAATAACTCCTTATTTAAGTTATTCATATGGAAAAGTAAGAAATAACAAAAATAATTCAAAATATAGAAAAGGATATATGTCAGGTGCTATATTAGGTTTAAGATATAATATGAAATATTTAGATTTAGATGTAGCTTATGCAAAACCttt                                                                                       >  2:889080/1‑251 (MQ=255)                          tggtTATCATAGAACTGGAAATATACAAGGTGATAAAGCAATGGAAATAGAAAATGAATTATCATATAGAGTGTTAGACTCAGAAAAGTTTGGAAAAATAACTCCTTATTTAAGTTATTCATATGGAAAAGTAAGAAATAACAAAAATAATTCAAAATATAGAAAAGGATATATGTCAGGTGCTATATTAGGTTTAAGATATAATATGAAATATTTAGATTTAGATGTAGCTTATGCAAAACCTTTAGCt                                                                                   <  2:769881/250‑1 (MQ=255)                          tggtTAACATAGAACTGGAAATATACAAGGTGATAAAGCAATGGAAATAGAAAATGAATTATCATATAGAGTGTTAGACTAAGAAAAGTTTGGAAAAATAACTCCTTATTTAAGTTATTCATATGGAAAAGTAAGAAATAACAAAAATAATTCAAAATATAGAAAAGGATATATGTCAGGTGCTATATTAGGTTTAAGATATAATATGAAATATTTAGATTTAGATGTAGCTTATGCAAAACCTTTAGCt                                                                                   <  2:19163/250‑1 (MQ=255)                                          ggAAATATACAAGGTGATAAAGCAATGGAAATAGAAAATGAATTATCATATAGAGTGTTAGACTCAGAAAAGTTTGGAAAAATAACTCCTTATTTAAGTTATTCATATGGAAAAGTAAGAAATAACAAAAATAATTCAAAATATAGAAAAGGATATATGTCAGGTGCTATATTAGGTTTAAGATATAATATGAAATATTTAGATTTAGATGTAGCTTATGCAAAACCTTTAGCTCGTTCAAATTACTTaaa                                                                  >  2:508245/1‑251 (MQ=255)                                           ggcatatACAAGGGGCTAAAGCAATGGAAAAAGAAAATGAATTATCATAAAGAGTGGTAGACTCAGAAAAGTTTGGAAAGAAAACTCCTTATTTAAGTTAGTCATATGGAAAAGCAAGGAATAACAAAAATAATTCAAAATATAGAAAAGGATATATGACAGGTGCTATATAAGGTTTAAGATATAATATGAAATATTTAGATTCAGATGTAGCTTATGCAAAACCTTTAGCTCGTTCAAATTACTTcaaa                                                                 >  2:117046/4‑251 (MQ=17)                                                    aaGGTGATAAAGCAATGGAAATAGAAAATGAATTATCATATAGAGTGTTAGACTCAGAAAAGTTTGGAAAAATAACTCCTTATTTAAGTTATTCATATGGAAAAGTAAGAAATAACAAAAATAATTCAAAATATAGAAAAGGATATATGTCAGGTGCTATATTAGGTTTAAGATATAATATGAAATATTTAGATTTAGATGTAGCTTATGCAAAACCTTTAGCTCGTTCAAATTACTTAAAACTTAAGAAt                                                        <  1:898957/251‑1 (MQ=255)                                                    aaGGTGATAAAGCAATGGAAATAGAAAATGAATTATCATATAGAGTGTTAGACTCAGAAAAGTTTGGAAAAATAACTCCTTATTTAAGTTATTCATATGGAAAAGTAAGAAATAACAAAAATAATTCAAAATATAGAAAAGGATATAAGTCAGGTGCTATATTAGGTTTAAGATATAATATGAAATATTTAGATTTAGATGTAGCTTATGCAAAACCTTTAGCTCGTTCAAATTACTTAAAACTTAAGAAt                                                        <  1:889080/251‑1 (MQ=255)                                                                cAATGGAAATAGAAAATGAATTATCATATAGAGTGTTAGACTCAGAAAAGTTTGGAAAAATAACTCCTTATTTAAGTTATTCATATGGAAAAGTAAGAAATAACAAAAATAATTCAAAATATAGAAAAGGATATATGTCAGGTGCTATATTAGGTTTAAGATATAATATGAAATATTTAGATTTAGATGTAGCTTATGCAAAACCTTTAGCTCGTTCAAATTACTTAAAACCTAAGAATAGAGAAatat                                              <  1:464016/249‑1 (MQ=255)                                                                      aaatagaaaATGAATTATCATATAGAGTGTTAGACTCAGAAAAGTCTGGAAAAATAACTCCTTATTTAAGTTATTCATATGGAAAAGTAAGAAATAACAAACATAATTCAAAATATAGAAAAGGATATATGTCAGGTGCTATATTAGGTTTAAGATATAATATGAAATATTTAGATTTAGATGTAGCTTATGCAAAACCTTTAGCTCGTTCAAATTACTTAAAACCTAAGAATAGAGAAATATATTTTAg                                       <  1:800410/250‑1 (MQ=255)                                                                      aaatagaaaATGAATTATCATATAGAGTGTTAGAATCAGAAAAGTTTGGAAAAATAACTCCTTATTTAAGTTATTCATATGGAAAAGTAAGAAATAACAAAAATAATTCAAAATATAGAAAAGGATATATGTCAGGTGCTATATTAGGTTTAAGATATAATATGAAATATTTAGATTTAGATGTAGCTTATGCAAAACCTTTAGCTCGTTCAAATTACTTAAAACCTAAGAATAGAGAAATATATTTTAg                                       <  2:269574/250‑1 (MQ=255)                                                                                 gAATTATCATATAGAGTGTTAGACTCAGAAAAGTTTGGAAAAATAACTCCTTATTTAAGTTATTCATATGGAAAAGTAAGAAATAACAAAAATAATTCAAAATATAGAAAAGGATATATGTCAGGTGCTATATTATGTTTAAGATATACTATGAAATATTTAGATTTAGATGTAGCTTATGCAAAACCTTTAGCTCGTTCAAATCACTTAAAACCTAAGAATAGAGAAATATATTTTAGTGCAACATTaaa                           >  1:815884/1‑251 (MQ=255)                                                                                    ttATCATGTAGAGTGTTAGACTCAGAAAAGTTTGGAAAAATAACTCCTTATTTAAGTTATTCATATGGAAAAGTAAGAAATAACAAAAATAATTCAAAATATATAAAAGGATATATGTCAGGTGCTATATTAGGTTTAAGATATAATATGAAATATTTAGATTTAGATGTAGCTTATGCAAAACCTTTAGCTCGTTCAAATTACTTAAAACCTAAGAATAGAGAAATATATTTTAGTGCAACATTAAaaat                        >  1:900411/1‑251 (MQ=255)                                                                                      aTCATATAGAGTGTTAGACTCAGAAAAGTTTGGAAAAATAACTCCTTATTTAAGTTATTCATATGGAAAAGTAAGAAATAACAAAAATAATTCAAAATATAGAAAAGGATATATGTCAGGTGCTATATTAGGTTTAAGATATAATATGAAATATTTAGATTTAGATGTAGCTTATGCAAAACCTTTAGCTCGTTCAAATTACTTAAAACCTAAGAAt                                                        >  1:521550/1‑217 (MQ=255)                                                                                      aTCATATAGAGTGTTAGACTCAGAAAAGTTTGGAAAAATAACTCCTTATTTAAGTTATTCATATGGAAAAGTAAGAAATAACAAAAATAATTCAAAATATAGAAAAGGATATATGTCAGGTGCTATATTAGGTTTAAGATATAATATGAAATATTTAGATTTAGATGTAGCTTATGCAAAACCTTTAGCTCGTTCAAATTACTTAAAACCTAAGAAt                                                        <  2:521550/217‑1 (MQ=255)                                                                                      aTCATATAGAGTGTTAGACTCAGAAAAGTTTGGAAAAATAACTCCTTATTTAAGTTATTCATATGGAAAAGTAAGAAATAACAAAAATAATTCAAAATATAGAAAAGGATATATGTCAGGTGCTATATTAGGTTTAAGATATAATATGAAATATTTAGATTTAGATGTAGCTTATGCAAAACCTTTAGCTCGTTCAAATTACTTAAAACCTAAGAATAgaga                                                   <  2:262318/222‑1 (MQ=255)                                                                                      aTCATATAGAGTGTTAGACTCAGAAAAGTTTGGAAAAATAACTCCTTATTTAAGTTATTCATATGGAAAAGTAAGAAATAACAAAAATAATTCAAAATATAGAAAAGGATATATGTCAGGTGCTATATTAGGTTTAAGATATAATATGAAATATTTAGATTTAGATGTAGCTTATGCAAAACCTTTAGCTCGTTCAAATTACTTAAAACCTAAGAATAgaga                                                   >  1:262318/1‑222 (MQ=255)                                                                                      aTCATATAGAGTGTTAGACTCAGAAAAGTTTGGAAAAATAACTCCTTATTTAAGTTATTCATATGGAAAAGTAAGAAATAACAAAAATAATTCAAAATATAGAAAAGGATATATGTCAGGTGCTATATTAGGTTTAAGATATAATATGAAATATTTAGATTTAGATGTAGCTTATGCAAAACCTTTAGCTCGTTCAAATTACTTAAAACCTAAGAATAGAGAAATATATTTTAGTGCAACATTAAaaatta                      >  2:707887/1‑251 (MQ=255)                                                                                      aTCATATAGAGTGTTAGACTCAGAAAAGTTTGGAAAAATAACTCCTTATTTAAGTTATTCATATGGAAAAGTAAGAAATAACAAAAATAATTCAAAATATAGAAAAGGATATATGTCAGGTGCTATATTAGGTTTAAGATATAATATGAAATATTTAGATTTAGATGTAGCTTATGCAAAACCTTTAGCTCGTTCAAATTACTTAAAACCTAAGAATAGAGAAATATATTTTAGTGCAACATTAAaaatta                      >  1:491458/1‑251 (MQ=255)                                                                                      aTCATATAGAGTGTTAGACTCAGAAAAGTTTGGAAAAATAACTCCTTATTTAAGTTATTCATATGGAAAAGTAAGAAATAACAAAAATAATTCAAAATATAGAAAAGGATATATGTCAGGTGCTATATTAGGTTTAAGATATAATATGAAATATTTAGATTTAGATGTAGCTTATGCAAAACCTTTAGCTCGTTCAAATTACTTAAAACCTAAGAATAGAGAAATATATTTTAGTGCAACATTAAaaatta                      >  2:897273/1‑251 (MQ=255)                                                                                      aTCATATAGAGTGTTAGACTCAGAAAAGTTTGGAAAAATAACTCCTTATTTAAGTTATTCATATGGAAAAGTAAGAAATAACAAAAATAATTCAAAATATAGAAAAGGATATATGTCAGGTGCTATATTAGGTTTAAGATATAATATGAAATATTTAGATTTAGATGTAGCTTATGCAAAACCTTTAGCTCGTTCAAATTACTTAAAACCCAAGAATAGAGAAATATATTTTAGTGCAACATTAAcaatta                      >  2:885699/1‑251 (MQ=255)                                                                                                gtgtTAGACTCAGAAAAGTTTGGAAAAATAACTCCTTATTTAAGTTATTCATATGGAAAAGTAAGAAATAACAAAAATAATTCAAAATATAGAAAAGGATATATGTCAGGTGCTATATTAGGTTTAAGATATAATATGAAATATTTAGATTTAGATGTAGCTTATGCAAAACCTTTAGCTCGTTCAAATTACTTAAAACCTAAGAATAGAGAAATATATTTTAGTGCAACATTAAAAATTAAATTTTAGAt            >  2:86857/1‑251 (MQ=255)                                                                                                  gtTAGACTCAGAAAAGTTTGGAAAAATAACTCCTTATTTAAGTTATTCATATGGAAAAGTAAGAAATAACAAAAATAATTCAAAATATAGAAAAGGATATATGTCAGGTGCTATATTAGGTTTAAGATATAATATGAAATATTTAGATTTAGATGTAGCTTATGCAAAACCTTTAGCTCGTTCAAATTACTTAAAACCTAAGAATAGAGAAATATATTTTAGTGCAACATTAAAAATTAAATTTTAGATgg          >  2:374506/1‑251 (MQ=255)                                                                                                          cAGAAAAGTTTGGAAAAATAACTCCTTATTTAAGTTATTCATATGGAAAAGTAAGAAATAACAAAAATAATTCAAAATATAGAAAAGGATATATGTCAGGTGCTATATTAGGTTTAAGATATAATATGAAATATTTAGATTTAGATGTAGCTTATGCAAAACCTTTAGCTCGTTCAAATTACTTAAAACCTAAGAATAGAGAAATATATTTTAGTGCAACATTAAAAATTAAATTTTAGATGGGAGGGAAt  >  1:49210/1‑251 (MQ=255)                                                                                                                 |                                                                                                                                                                                                                                                    CAATAGGTGGAGTAGGAAGTGTTGGTGGTTATCATAGAACTGGAAATATACAAGGTGATAAAGCAATAGAAATAGAAAATGAATTATCATATAGAGTGTTAGACTCAGAAAAATTTGGAAAAATAACTCCTTATTTAAGTTATTCATATGGAAAAGTAAGAAATAACAAAAATAATTCAAAATATAGAAAAGGATATATGTCAGGTGCTATATTAGGTTTAAGATATAATATGAAATATTTAGATTTAGATGTAGCTTATGCAAAACCTTTAGCTCGTTCAAATTACTTAAAACCTAAGAATAGAGAAATATATTTTAGTGCAACATTAAAAATTAAATTTTAGATGGGAGGGAAT  >  CP028101/760950‑761305

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑