Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP028101	761,017	A→G	I464M (ATA→ATG)	C7Y58_03755 →	hemolysin activator protein precursor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP028101	761,017	0	A	G	100.0%	58.1 / NA	18	I464M (ATA→ATG)	C7Y58_03755	hemolysin activator protein precursor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (8/10);  total (8/10)

CAATAGGTGGAGTAGGAAGTGTTGGTGGTTATCATAGAACTGGAAATATACAAGGTGATAAAGCAATAGAAATAGAAAATGAATTATCATATAGAGTGTTAGACTCAGAAAAATTTGGAAAAATAACTCCTTATTTAAGTTATTCATATGGAAAAGTAAGAAATAACAAAAATAATTCAAAATATAGAAAAGGATATATGTCAGGTGCTATATTAGGTTTAAGATATAATATGAAATATTTAGATTTAGATGTAGCTTATGCAAAACCTTTAGCTCGTTCAAATTACTTAAAACCTAAGAATAGAGAAATAT  >  CP028101/760950‑761261                                                                    |                                                                                                                                                                                                                                                     cAATAGGTGGAGTAGGAAGTGTTGGTGGTTATCATAGAACTGGAAATATACAAGGTGATAAAGCAATGGAAATAGAAAATGAATTATCATATAGAGTGTTAGACTCAGAAAAGTTTGGAAAAATAACTCCTTATTTAAGTTATTCATATGGAAAAGTAAGAAATAACAAAAATAATTCAAAATATAGAAAAGGATATATGTCAGGTGCTATATTAGGTTTAAGATATAATATGAAATATttagatttagat                                                               <  2:149747/251‑1 (MQ=18)  aaTAGGTGGAGTAGGAAGTGTTGGTGGTTATCATAGAACTGGAAATATACAAGGTGATAAAGCAATGGAAATAGAAAATGACTTATCATATAGAGTGTTAGACTCAGAAAAGTTTGGAAAAATAACTCCTTATTTAAGTTATTCATATGGAAAAGTAAGAAATAACAAAAATAATTCAAAATATAGAAAAGGATATATGTCAGGTGCTATATTAGGTTTAAGATATAATATGAAATATTTAGATTTAGATg                                                              <  1:787873/251‑1 (MQ=21)       gTGGAGTAGGAAGTGTTGGTGGTTATCATAGAACTGGAAATATACAAGGTGATAAAGCAATGGAAATAGAAAATGAATTATCATATAGAGTGTTAGACTCAGAAAAGTTTGGAAAAATAACTCCTTATTTAAGTTATTCATATGGAAAAGTAAGAAATAACAAAAATAATTCAAAATATAGAAAAGGATATATGTCAGGTGCTATATTAGGTTTAAGATATAATATGAAATATTTAGATTTAGATGTAg                                                           >  2:800410/1‑249 (MQ=25)        tGGAGTTGGAAGTGTTGGTGGTTATCATAGAACTGGAAATATACAAGGTGATAAAGCAATGGAAATAGAAAATGAATTATCATATAGAGTGTTAGACTCAGAAAAGTTTGGAAACATAACTCCTTATTTAAGTTATTCATATGGAAAAGTAAGAAATAACAAAAATAATTCAAAATATAGAAAAGGATATATGTCAGGTGCTATATTAGGTTTAAGATATAATATGAAATATTTAGATTTAGATGTAGCt                                                         <  1:834216/250‑1 (MQ=255)        tGGAGTAGGAAGTGTTGGTGGTTATCATAGAACTGGAAATATACAAGGTGATAAAGCAATGGAAATAGAAAATGAATTATCATATAGAGTGTTAGACTCAGAAAAGTTTGGAAAAATAACTCCTTATTTAAGTTATTCATATGGAAAAGTAAGAAATAACAAAAATAATTCAAAATATAGAAAAGGATATATGTCAGGTGCTATATTAGGTTTAAGATATAATATGAAATATTTAGATTTAGATGTAGCt                                                         <  2:892798/250‑1 (MQ=37)          gAGTAGGAAGTGTTGGTGGTTATCATAGAACTGGAAATATACAAGGTGATAAAGCAATGGAAATAGAAAATGAATTATCATATAGAGTGTTAGACTCAGAAAAGTTTGGAAAAATAACTCCTTATTTAAGTTATTCATATGGAAAAGTAAGAAATAACAAAAATAATTCAAAATATAGAAAAGGATATATGTCAGGTGCTATATTAGGTTTAAGATATAATATGAAATATTTAGATTTAGATGTAGCTTAt                                                      >  2:898957/1‑251 (MQ=255)           aGTAGGAAGTGTTGGTGGTTATCATAGAACTGGAAATATACAAGGTGATAAAGCAATGGAAATAGAAAATGAATTATCATATAGAGTGTTAGACTCAGAAAAGTTTGGAAAAATAACTCCTTATTTAAGTTATTCATATGGAAAAGTAAGAAATAACAAAAATAATTCAAAATATAGAAAAGGATATATGTCAGGTGCTATATTAGGTTTAAGATATAATATGAAATATTTAGATTTAGATGTAGCTTATg                                                     >  2:676437/1‑251 (MQ=255)             tAGGAAGTGTTGGTGGTTATCATAGAACTGGAAATATGCAAGGTGATAAAGCAATGGACATAGAAAATGAATTAGCATATCGAGTGTTAGACTCAGAAAAGTTTGGAAAAATAACTCCTTATTTAAGTTATTCATATGGAAAAGTAAGAAATAACAAAAATAATTCAAAATATAGAAAAGGATATATGTCAGGTGCTATATTAGGTTTAAGATATAATATGAAATATTTAGATTTAGATGTAGCTTATGc                                                    <  1:676437/250‑1 (MQ=255)                gAAGTGTTGGTGGTTATCATAGAACTGGAAATATACAAGGTGATAAAGCAATGGAAATAGAAAATGAATTATCATATAGAGTGTTAGACTCAGAAAAGTTTGGAAAAATAACTCCTTATTTAAGTTATTCATATGGAAAAGTAAGAAATAACAAAAATAATTCAAAATATAGAAAAGGATATATGTCAGGTGCTATATTAGGTTTAAGATATAATATGAAATATTTAGATTTAGATGTAGCTTATGCaaaa                                                >  1:384413/1‑251 (MQ=255)                   gtgtTGGTGGTTATCATAGAACTGGAAATATACAAGGTGATAAAGCAATGGAAATAGAAAATGAATTATCATATAGAGTGTTAGACTCAGAAAAGTTTGGAAAAATAACTCCTTATTTAAGTTATTCATATGGAAAAGTAAGAAATAACAAAAATAATTCAAAATATAGAAAAGGATATATGTCAGGGGCTATATTAGGATTAAGATATAATATGAAATATTTAGATTTAGATGTAGCTTATGCAAAAc                                               >  1:269574/1‑249 (MQ=255)                     gtTGGTGGTTATCATAGAACTGGAAATATACAAGGTGATAAAGCAATGGAAATAGAAAATGAATTATCATATAGAGTGTTAGACTCAGAAAAGTTTGGAAAAATAACTCCTTATTTAAGTTATTCATATGGAAAAGTAAGAAATAACAAAAATAATTCAAAATATAGAAAAGGATATATGTCAGGTGCTATATTAGGTTTAAGATATAATATGAAATATTTAGATTTAGATGTAGCTTATGCAAAACCttt                                           >  2:889080/1‑251 (MQ=255)                          tggtTATCATAGAACTGGAAATATACAAGGTGATAAAGCAATGGAAATAGAAAATGAATTATCATATAGAGTGTTAGACTCAGAAAAGTTTGGAAAAATAACTCCTTATTTAAGTTATTCATATGGAAAAGTAAGAAATAACAAAAATAATTCAAAATATAGAAAAGGATATATGTCAGGTGCTATATTAGGTTTAAGATATAATATGAAATATTTAGATTTAGATGTAGCTTATGCAAAACCTTTAGCt                                       <  2:769881/250‑1 (MQ=255)                          tggtTAACATAGAACTGGAAATATACAAGGTGATAAAGCAATGGAAATAGAAAATGAATTATCATATAGAGTGTTAGACTAAGAAAAGTTTGGAAAAATAACTCCTTATTTAAGTTATTCATATGGAAAAGTAAGAAATAACAAAAATAATTCAAAATATAGAAAAGGATATATGTCAGGTGCTATATTAGGTTTAAGATATAATATGAAATATTTAGATTTAGATGTAGCTTATGCAAAACCTTTAGCt                                       <  2:19163/250‑1 (MQ=255)                                          ggAAATATACAAGGTGATAAAGCAATGGAAATAGAAAATGAATTATCATATAGAGTGTTAGACTCAGAAAAGTTTGGAAAAATAACTCCTTATTTAAGTTATTCATATGGAAAAGTAAGAAATAACAAAAATAATTCAAAATATAGAAAAGGATATATGTCAGGTGCTATATTAGGTTTAAGATATAATATGAAATATTTAGATTTAGATGTAGCTTATGCAAAACCTTTAGCTCGTTCAAATTACTTaaa                      >  2:508245/1‑251 (MQ=255)                                           ggcatatACAAGGGGCTAAAGCAATGGAAAAAGAAAATGAATTATCATAAAGAGTGGTAGACTCAGAAAAGTTTGGAAAGAAAACTCCTTATTTAAGTTAGTCATATGGAAAAGCAAGGAATAACAAAAATAATTCAAAATATAGAAAAGGATATATGACAGGTGCTATATAAGGTTTAAGATATAATATGAAATATTTAGATTCAGATGTAGCTTATGCAAAACCTTTAGCTCGTTCAAATTACTTcaaa                     >  2:117046/4‑251 (MQ=17)                                                    aaGGTGATAAAGCAATGGAAATAGAAAATGAATTATCATATAGAGTGTTAGACTCAGAAAAGTTTGGAAAAATAACTCCTTATTTAAGTTATTCATATGGAAAAGTAAGAAATAACAAAAATAATTCAAAATATAGAAAAGGATATATGTCAGGTGCTATATTAGGTTTAAGATATAATATGAAATATTTAGATTTAGATGTAGCTTATGCAAAACCTTTAGCTCGTTCAAATTACTTAAAACTTAAGAAt            <  1:898957/251‑1 (MQ=255)                                                    aaGGTGATAAAGCAATGGAAATAGAAAATGAATTATCATATAGAGTGTTAGACTCAGAAAAGTTTGGAAAAATAACTCCTTATTTAAGTTATTCATATGGAAAAGTAAGAAATAACAAAAATAATTCAAAATATAGAAAAGGATATAAGTCAGGTGCTATATTAGGTTTAAGATATAATATGAAATATTTAGATTTAGATGTAGCTTATGCAAAACCTTTAGCTCGTTCAAATTACTTAAAACTTAAGAAt            <  1:889080/251‑1 (MQ=255)                                                                cAATGGAAATAGAAAATGAATTATCATATAGAGTGTTAGACTCAGAAAAGTTTGGAAAAATAACTCCTTATTTAAGTTATTCATATGGAAAAGTAAGAAATAACAAAAATAATTCAAAATATAGAAAAGGATATATGTCAGGTGCTATATTAGGTTTAAGATATAATATGAAATATTTAGATTTAGATGTAGCTTATGCAAAACCTTTAGCTCGTTCAAATTACTTAAAACCTAAGAATAGAGAAatat  <  1:464016/249‑1 (MQ=255)                                                                    |                                                                                                                                                                                                                                                     CAATAGGTGGAGTAGGAAGTGTTGGTGGTTATCATAGAACTGGAAATATACAAGGTGATAAAGCAATAGAAATAGAAAATGAATTATCATATAGAGTGTTAGACTCAGAAAAATTTGGAAAAATAACTCCTTATTTAAGTTATTCATATGGAAAAGTAAGAAATAACAAAAATAATTCAAAATATAGAAAAGGATATATGTCAGGTGCTATATTAGGTTTAAGATATAATATGAAATATTTAGATTTAGATGTAGCTTATGCAAAACCTTTAGCTCGTTCAAATTACTTAAAACCTAAGAATAGAGAAATAT  >  CP028101/760950‑761261

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑