| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP028101 | 514,508 | G→A | L2237L (TTG→TTA) | C7Y58_02630 → | autotransporter domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP028101 | 514,508 | 0 | G | A | 92.3% | 19.1 / ‑3.5 | 13 | coding (6711/7290 nt) | C7Y58_02630 | autotransporter domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (9/3); minor base . (1/0); total (10/3) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATTTGGAACTAATGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAAGATATAGGAAAATCAAAAGAACATATGTTACAAGCAAAGGTAGGATTATTAAAATCAGTGCCATTTGATGATAACAATAGCTTGAATTG‑GACAATATCAGGAGATATCTTTGTAGGACGTAATAGAATGCATAGAAAATACTTAGTAGTGGATGAAATATTTAATGCAAAAGCAAGATACTATACTTATGGAATAGGAGTAAAGAATGAAATAGGTAAAGAATTCAGATTAAGTGAATCATTCACATTAAGACCATATGCAGCATTAAAGTTAGAATATGGAAGAGTATCAAAGATAAGAGAAAAATCAGGAGAAATCAAGTTAG > CP028101/514265‑514749 | aTTTGGAACTAATGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAAGATATAGGAAAATCAAAAGAACATATGTTACAAGCAAAGGTAGGATTATTAAAATCAGTGCAATTTGATGATAACAATAGCTTAAATTG‑Ga > 2:463695/1‑251 (MQ=18) gAACTAATGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGTGGTATTGGTCGTTTCACAGGTATTTTTCATAATAACTTTTCGTTCAAAGCTCTCTTAAAATCACCCTCCCATATGTTATAATTTAAGGTAGTTTTCTTAAAATCATTGCCATTTGATGATAACAATAGCTT‑AATTGTGACAata > 1:402895/1‑251 (MQ=2) gAACTAATGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAAGATATAGGAAAATCAAAAGAACATATGTTACAAGCAAAGGTAGGATTATTAAAATCAGTGCCATTTGATGATAACAATAGCTTAAATTG‑GACAata > 1:171546/1‑251 (MQ=18) gAACTAATGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAAGATATAGGAAAATCAAAAGAACATATGTTACAAGCAAAGGTAGGATTATTAAAATCAGTGCCATTTGATGATAACAATAGCTTAAATTG‑GACAata > 1:298711/1‑251 (MQ=18) gAACTAATGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAAGATATAGGAAAATCAAAAGAACATATGTTACAAGCAAAGGTAGGATTATTAAAATCAGTGCCATTTGATGATAACAATAGCTTAAATTG‑GACAata > 2:385827/1‑251 (MQ=18) cTAATGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAAGATATAGGAAAATCAAAAGAACATATGTTACAAGCAAAGGTAGGATTATTAAAATCAGTGCCATTTGATGATAACAATAGCTTAAATTG‑GACAATATCa > 1:59356/1‑251 (MQ=14) cTAATGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAAGATATAGGAAAATCAAAAGAACATATGTTACAAGCAAAGGTAGGATTATTAAAATCAGTGCCATTTGATGATAACAATAGCTTAAATTG‑GACAATATCa > 2:229637/1‑251 (MQ=14) aTGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAAGATATAGGAAAATCAAAAGAACATATGTTACAAGCAAAGGTAGGATTATTAAAATCAGTGCCATTTGATGATAACAATAGCTTAAATTG‑GACAATATCAGga < 1:21235/251‑1 (MQ=11) aTGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAAGATATAGGAAAATCAAAAGAACATATGTTACAAGCAAAGGTAGGATTATTAAAATCAGTGCCATTTGATGATAACAATAGCTTAAATTG‑GACAATATCAGga < 1:533890/251‑1 (MQ=11) tAACAATAGCTTAAATTG‑GACAATATCAGGAGATATCTTTGTAGGACGTAATAGAATGCATAGAAAATACTTAGTAGTGGATGAAATATTTAATGCAAAAGCAAGATACTATACTTATGGAATAGGAGTAAAGAATGAAATAGGTAAAGAATTCAGATTAAGTGAATCATTCACATTAAGACCATATGCAGCATTAAAGTTAGAATATGGAAGAGTATCAAAGATAAGAGAAAAATCAGGAGAAATCAAGt > 1:21794/1‑251 (MQ=14) tAACAATAGCTTAAATTG‑GACAATATCAGGAGATATCTTTGTAGGACGTAATAGAATGCATAGAAAATACTTAGTAGTGGATGAAATATTTAATGCAAAAGCAAGATACTATACTTATGGAATAGGAGTAAAGAATGAAATAGGTAAAGAATTCAGATTAAGTGAATCATTCACATTAAGACCATATGCAGCATTAAAGTTAGAATATGGAAGAGTATCAAAGATAAGAGAAAAATCAGGAGAAATCAAGt > 2:599005/1‑251 (MQ=14) aCAATAGCTTAAATTG‑GACAATATCAGGAGATATCTTTGTAGGACGTAATAGAATGCATAGAAAATACTTAGTAGTGGATGAAATATTTAATGCAAAAGCAAGATACTATACTTATGGAATAGGAGTAAAGAATGAAATAGGTAAAGAATTCAGATTAAGTGAATCATTCACATTAAGACCATATGCAGCATTAAAGTTAGAATATGGAAGAGTATCAAAGATAAGAGAAAAATCAGGAGAAATCAAGTTa < 1:91969/251‑1 (MQ=14) cAATAGCTTAAATTG‑GACAATATCAGGAGATATCTTTGTAGGACGTAATAGAATGCATAGAAAATACTTAGTAGTGGATGAAATATTTAATGCAAAAGCAAGATACTATACTTATGGAATAGGAGTAAAGAATGAAATAGGTAAAGAATTCAGATTAAGTGAATCATTCACATTAAGACCATATGCAGCATTAAAGTTAGAATATGGAAGAGTATCAAAGATAAGAGAAAAATCAGGAGAAATCAAGTTAg > 1:501873/1‑251 (MQ=14) | ATTTGGAACTAATGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAAGATATAGGAAAATCAAAAGAACATATGTTACAAGCAAAGGTAGGATTATTAAAATCAGTGCCATTTGATGATAACAATAGCTTGAATTG‑GACAATATCAGGAGATATCTTTGTAGGACGTAATAGAATGCATAGAAAATACTTAGTAGTGGATGAAATATTTAATGCAAAAGCAAGATACTATACTTATGGAATAGGAGTAAAGAATGAAATAGGTAAAGAATTCAGATTAAGTGAATCATTCACATTAAGACCATATGCAGCATTAAAGTTAGAATATGGAAGAGTATCAAAGATAAGAGAAAAATCAGGAGAAATCAAGTTAG > CP028101/514265‑514749 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |