| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP028101 | 880,807 | T→A | H1893Q (CAT→CAA) | C7Y58_04335 → | autotransporter domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP028101 | 880,807 | 0 | T | A | 97.1% | 104.6 / ‑3.5 | 34 | coding (5679/6318 nt) | C7Y58_04335 | autotransporter domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base A (20/13); minor base . (1/0); total (21/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.24e-01 | |||||||||||
ATCTAAGAGATGAATGGAGAACAGCTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAAGATATAGGAAAATCAAAAGAA‑CATATGTTACAAGCAAAGGTAGGATTATTAAAATCAGTGCCATTTGATGATAACAATAGCTTGAATTGGACAATATCAGGA > CP028101/880572‑880885 | atCTAAGAGATGAATGGAGAACAGCTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAA‑CAAATGTTGCAAGCAAAg > 2:461782/1‑251 (MQ=255)atCTAAGAGATGAATGGAGAACAGCTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAA‑CAAATGTTGCAAGCAAAg > 1:370958/1‑251 (MQ=255) tCTAAGAGATGAATGGAGAACAGCTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGGAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTCGATGGTACACAGGTATAGTTCATAATACATTTAACTTCAAGGATATAGGAAATTCAAAAGAA‑CAAATGTTGCAAGCAAAgg > 1:739370/1‑251 (MQ=14) gaTGAATGGAGAACAGCTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAA‑CAAATGTTGCAAGCAAAGGTAGGatt > 2:504018/1‑251 (MQ=255) gAATGGAGAACAGCTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAA‑CAAATGTTGCAAGCAAAGGTAGGattatt > 2:231010/1‑251 (MQ=255) aTGGAGAACAGCTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATCGGAAATTCAAAAGAA‑CAAATGTTGCAAGCAAAGGTAGGATTATTaa > 1:798096/1‑251 (MQ=255) aTGGAGAACAGCTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAA‑CAAATGTTGCAAGCAAAGGTAGGATTATTaa < 1:286194/251‑1 (MQ=255) ggAGAACAGCTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAA‑CAAATGTTGCAAGCAAAGGTAGGATTATTaaaa > 1:883191/1‑251 (MQ=255) ggAGAACAGCTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAA‑CAAATGATGCAAGCAAAGGTAGGATTATTaaaa > 2:220478/1‑251 (MQ=255) gagaACAGCTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAA‑CAAATGTTGCAAGCAAAGGTAGGATTATTaaaa < 1:461782/250‑1 (MQ=255) gaACAGCTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGTTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAA‑CAAATGTTGCAAGCAAAGGTAGGACTATTAAACTCa > 2:607398/1‑251 (MQ=14) aaCAGCTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAA‑CAAATGTTGCAAGCAAAGGTAGGATTATTAAAATc < 2:101559/249‑1 (MQ=255) cAGCTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAA‑CAAATGTTGCAAGCAAAGGTAGGAttatta > 2:203703/1‑242 (MQ=255) cAGCTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAA‑CAAATGTTGCAAGCAAAGGTAGGAttatta < 1:203703/242‑1 (MQ=255) gCTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGCATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGGATAGTTAATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAAACA‑ATGTTGCAAGCAAAGGTAGGATTATTAAAATCAGTGca > 2:532968/1‑250 (MQ=11) gCTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTACGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAA‑CAAATGTTGCAAGCAAAGGTAGGATTATTAAAATCAGTGc > 1:828486/1‑250 (MQ=255) cTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAA‑CAAATGTTGCAAGCAAAGGTAGGATTATTAAAATCAGTGCCa > 2:796339/1‑251 (MQ=255) cTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAA‑CAAATGTTGCAAGCAAAGGTAGGATTATTAAAATCAGTGCCa > 1:543362/1‑251 (MQ=255) cAAATAACATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAA‑CAAATGTTGCAAGCAAAGGTAGGATTATTAAAATCAGTGCCATTTGATGATAAc < 1:835803/251‑1 (MQ=255) ataaaataaaAGCATTTGGAACTAGAGGAGAATACAAAACAGATACTGCAGGAGTTATAGACTACTAGAATCATGCTTATGGAGTAGCTTATGTACATGAAATTGAAGATATTAAACTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAA‑CAAATGTTGCAAGCAAAGGTAGGATTATTAAAATCAGTGCCATTTGATGATAACAAt < 1:777737/251‑1 (MQ=11) ataaaataaaAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAA‑CAAATGTTGCAAGCAAAGGTAGGATTATTAAAATCAGTGCCATTTGATGATAACAAt < 2:370958/251‑1 (MQ=255) ataaaataaaAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAA‑CAAATGTTGCAAGCAAAGGTAGGATTATTAAAATCAGTGCCATTTGATGATAACAAt < 2:899525/251‑1 (MQ=255) ataaaataaaAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAA‑CAAATGTTGCAAGCAAAGGTAGGATTATTAAAATCAGTGCCATTTGATGATAACAAt < 1:818225/251‑1 (MQ=255) ataaaaGCATTTGGAACAAGAGGAGAATACAAAACAGATACAGCAGGAGATATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGTAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAA‑CAAATGTTGAAAGCAAAGGTAGGTTTATTAAAATCAGCGCAATTTGATGATAACATTAGCtt > 2:744043/1‑251 (MQ=11) aaGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTGATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGTGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAA‑CAAATGTTGCAAGCAAAGGTAGGATTATTAAAATCAGTGCCATTTGATGATAACAATAGCTTGAAt < 1:775252/251‑1 (MQ=255) gCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAA‑CAAATGTTGCAAGCAAAGGTAGGATTATTAAAATCAGTGCCATTTGATGATAACAATAGCTTGAATTg > 2:569215/1‑251 (MQ=255) tttGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAACAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAA‑CAAATGTTGCAAGCAAAGGTAGGATTATTAAAATCAGTGCCATTTGATGATAACAATAGCTTGAATTGGAc < 1:638722/251‑1 (MQ=255) tGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAA‑CAAATGTTGCAAGCAAAGGTAGGATTATTAAAATCAGTGCCATTTGATGATAACAATAGCTTGAATTGGACaa > 1:526017/1‑251 (MQ=16) gAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAA‑CAAATGTTGCAAGCAAAGGTAGGATTATTAAAATCAGTGCCATTTGATGATAACAATAGCTTGAATTGGACAata > 1:83957/1‑251 (MQ=16) aaCTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAA‑CAAATGTTGCAAGCAAAGGTAGGATTATTAAAATCAGTGCCATTTGATGATAACAATAGCTTGAATTGGACAatat > 2:406275/1‑251 (MQ=12) aaCTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAA‑CAAATGTTGCAAGCAAAGGTAGGATTATTAAAATCAGTGCCATTTGATGATAACAATAGCTTGAATTGGACAatat > 1:65392/1‑251 (MQ=12) cTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAA‑CAAATGTTGCAAGCAAAGGTAGGATTATTAAAATCAGTGCCATTTGATGATAACAATAGCTTGAATTGGACAATATCa > 2:419122/1‑251 (MQ=9) aggagAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAA‑CAAATGTTGCAAGCAAAGGTAGGATTATTAAAATCAGTGCCATTTGATGATAACAATAGCTTGAATTGGACAATATCAGga < 1:867870/250‑1 (MQ=2) aggagAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAA‑CAAATGTTGCAAGCAAAGGTAGGATTATTAAAATCAGTGCCATTTGATGATAACAATAGCTTGAATTGGACAATATCAGga < 1:220478/250‑1 (MQ=2) | ATCTAAGAGATGAATGGAGAACAGCTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAAGATATAGGAAAATCAAAAGAA‑CATATGTTACAAGCAAAGGTAGGATTATTAAAATCAGTGCCATTTGATGATAACAATAGCTTGAATTGGACAATATCAGGA > CP028101/880572‑880885 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |