Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP028101	880,795	A→T	K1889N (AAA→AAT)	C7Y58_04335 →	autotransporter domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP028101	880,795	0	A	T	100.0%	116.0 / NA	36	K1889N (AAA→AAT)	C7Y58_04335	autotransporter domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (22/14);  total (22/14)

ATCTTAGATAAAGAATTTAAATATCTAAGAGATGAATGGAGAACAGCTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAAGATATAGGAAAATCAAAAGAA‑CATATGTTACAAGCAAAGGTAGGATTATTAAAATCAGTGCCATTTGATGATAACAATAGCTTGAATTGGACAATATCAGGA  >  CP028101/880550‑880885                                                                                                                                                                                                                                                      |                                                                                            atCTTAGATAAAGAATTTAAATATCTAAGAGATGAATGGAGAACAGCTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATACTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACATGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCaaa                                                                                        >  1:804444/1‑251 (MQ=255)   cTTAGATAAAGAATTTAAATATCTAAGAGATGAATGGAGAACAGCTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCaaaaa                                                                                      <  2:490726/251‑2 (MQ=255)                       atCTAAGAGATGAATGGAGAACAGCTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAA‑CAAATGTTGCAAGCAAAg                                                                 >  2:461782/1‑251 (MQ=255)                       atCTAAGAGATGAATGGAGAACAGCTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAA‑CAAATGTTGCAAGCAAAg                                                                 >  1:370958/1‑251 (MQ=255)                        tCTAAGAGATGAATGGAGAACAGCTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGGAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTCGATGGTACACAGGTATAGTTCATAATACATTTAACTTCAAGGATATAGGAAATTCAAAAGAA‑CAAATGTTGCAAGCAAAgg                                                                >  1:739370/1‑251 (MQ=14)                               gaTGAATGGAGAACAGCTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAA‑CAAATGTTGCAAGCAAAGGTAGGatt                                                         >  2:504018/1‑251 (MQ=255)                                  gAATGGAGAACAGCTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAA‑CAAATGTTGCAAGCAAAGGTAGGattatt                                                      >  2:231010/1‑251 (MQ=255)                                    aTGGAGAACAGCTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATCGGAAATTCAAAAGAA‑CAAATGTTGCAAGCAAAGGTAGGATTATTaa                                                    >  1:798096/1‑251 (MQ=255)                                    aTGGAGAACAGCTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAA‑CAAATGTTGCAAGCAAAGGTAGGATTATTaa                                                    <  1:286194/251‑1 (MQ=255)                                      ggAGAACAGCTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAA‑CAAATGTTGCAAGCAAAGGTAGGATTATTaaaa                                                  >  1:883191/1‑251 (MQ=255)                                      ggAGAACAGCTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAA‑CAAATGATGCAAGCAAAGGTAGGATTATTaaaa                                                  >  2:220478/1‑251 (MQ=255)                                       gagaACAGCTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAA‑CAAATGTTGCAAGCAAAGGTAGGATTATTaaaa                                                  <  1:461782/250‑1 (MQ=255)                                         gaACAGCTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGTTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAA‑CAAATGTTGCAAGCAAAGGTAGGACTATTAAACTCa                                               >  2:607398/1‑251 (MQ=14)                                          aaCAGCTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAA‑CAAATGTTGCAAGCAAAGGTAGGATTATTAAAATc                                                <  2:101559/249‑1 (MQ=255)                                            cAGCTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAA‑CAAATGTTGCAAGCAAAGGTAGGAttatta                                                     <  1:203703/242‑1 (MQ=255)                                            cAGCTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAA‑CAAATGTTGCAAGCAAAGGTAGGAttatta                                                     >  2:203703/1‑242 (MQ=255)                                              gCTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGCATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGGATAGTTAATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAAACA‑ATGTTGCAAGCAAAGGTAGGATTATTAAAATCAGTGca                                          >  2:532968/1‑250 (MQ=11)                                              gCTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTACGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAA‑CAAATGTTGCAAGCAAAGGTAGGATTATTAAAATCAGTGc                                           >  1:828486/1‑250 (MQ=255)                                               cTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAA‑CAAATGTTGCAAGCAAAGGTAGGATTATTAAAATCAGTGCCa                                         >  1:543362/1‑251 (MQ=255)                                               cTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAA‑CAAATGTTGCAAGCAAAGGTAGGATTATTAAAATCAGTGCCa                                         >  2:796339/1‑251 (MQ=255)                                                           cAAATAACATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAA‑CAAATGTTGCAAGCAAAGGTAGGATTATTAAAATCAGTGCCATTTGATGATAAc                             <  1:835803/251‑1 (MQ=255)                                                              ataaaataaaAGCATTTGGAACTAGAGGAGAATACAAAACAGATACTGCAGGAGTTATAGACTACTAGAATCATGCTTATGGAGTAGCTTATGTACATGAAATTGAAGATATTAAACTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAA‑CAAATGTTGCAAGCAAAGGTAGGATTATTAAAATCAGTGCCATTTGATGATAACAAt                          <  1:777737/251‑1 (MQ=11)                                                              ataaaataaaAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAA‑CAAATGTTGCAAGCAAAGGTAGGATTATTAAAATCAGTGCCATTTGATGATAACAAt                          <  2:899525/251‑1 (MQ=255)                                                              ataaaataaaAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAA‑CAAATGTTGCAAGCAAAGGTAGGATTATTAAAATCAGTGCCATTTGATGATAACAAt                          <  2:370958/251‑1 (MQ=255)                                                              ataaaataaaAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAA‑CAAATGTTGCAAGCAAAGGTAGGATTATTAAAATCAGTGCCATTTGATGATAACAAt                          <  1:818225/251‑1 (MQ=255)                                                                   ataaaaGCATTTGGAACAAGAGGAGAATACAAAACAGATACAGCAGGAGATATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGTAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAA‑CAAATGTTGAAAGCAAAGGTAGGTTTATTAAAATCAGCGCAATTTGATGATAACATTAGCtt                     >  2:744043/1‑251 (MQ=11)                                                                       aaGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTGATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGTGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAA‑CAAATGTTGCAAGCAAAGGTAGGATTATTAAAATCAGTGCCATTTGATGATAACAATAGCTTGAAt                 <  1:775252/251‑1 (MQ=255)                                                                         gCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAA‑CAAATGTTGCAAGCAAAGGTAGGATTATTAAAATCAGTGCCATTTGATGATAACAATAGCTTGAATTg               >  2:569215/1‑251 (MQ=255)                                                                            tttGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAACAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAA‑CAAATGTTGCAAGCAAAGGTAGGATTATTAAAATCAGTGCCATTTGATGATAACAATAGCTTGAATTGGAc            <  1:638722/251‑1 (MQ=255)                                                                              tGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAA‑CAAATGTTGCAAGCAAAGGTAGGATTATTAAAATCAGTGCCATTTGATGATAACAATAGCTTGAATTGGACaa          >  1:526017/1‑251 (MQ=16)                                                                                gAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAA‑CAAATGTTGCAAGCAAAGGTAGGATTATTAAAATCAGTGCCATTTGATGATAACAATAGCTTGAATTGGACAata        >  1:83957/1‑251 (MQ=16)                                                                                 aaCTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAA‑CAAATGTTGCAAGCAAAGGTAGGATTATTAAAATCAGTGCCATTTGATGATAACAATAGCTTGAATTGGACAatat       >  2:406275/1‑251 (MQ=12)                                                                                 aaCTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAA‑CAAATGTTGCAAGCAAAGGTAGGATTATTAAAATCAGTGCCATTTGATGATAACAATAGCTTGAATTGGACAatat       >  1:65392/1‑251 (MQ=12)                                                                                   cTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAA‑CAAATGTTGCAAGCAAAGGTAGGATTATTAAAATCAGTGCCATTTGATGATAACAATAGCTTGAATTGGACAATATCa     >  2:419122/1‑251 (MQ=9)                                                                                       aggagAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAA‑CAAATGTTGCAAGCAAAGGTAGGATTATTAAAATCAGTGCCATTTGATGATAACAATAGCTTGAATTGGACAATATCAGga  <  1:867870/250‑1 (MQ=2)                                                                                       aggagAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAA‑CAAATGTTGCAAGCAAAGGTAGGATTATTAAAATCAGTGCCATTTGATGATAACAATAGCTTGAATTGGACAATATCAGga  <  1:220478/250‑1 (MQ=2)                                                                                                                                                                                                                                                      |                                                                                            ATCTTAGATAAAGAATTTAAATATCTAAGAGATGAATGGAGAACAGCTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAAGATATAGGAAAATCAAAAGAA‑CATATGTTACAAGCAAAGGTAGGATTATTAAAATCAGTGCCATTTGATGATAACAATAGCTTGAATTGGACAATATCAGGA  >  CP028101/880550‑880885

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑