| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP028101 | 1,228,379 | 0 | T | . | 43.2% | 74.0 / 54.3 | 37 | intergenic (‑122/‑108) | C7Y58_05905/C7Y58_05910 | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (11/10); new base . (8/8); total (19/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.73e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CATTATAAAAATAAGAATTATAATAAAGTTCATTATTTTCCCACTTTATATCAAGTTTAATACTTCCATTAGGATAACTTTTAAAAAAATCTGCTGGACTTGCAAAAACATTCACACAAAATACAAAAAATAAAGTCAAAATTAAAAAAATCTTTTTCATAAACTCCTCTCATATTTCTAAAATAATTTATTATAAAATCATCTGTTCTATTATA‑GTCGTT‑TTTTTTTTTTTTTTTT‑CAAGCACTGTTTGAAAATATATTT‑AAAAAATATATCTAAAATAAAAATTATTTTCTTATGCTATAATTAAAATAAAGAATATTTTTGGAAAGGATTAAAAATATATTATGAATGAAGTAATATTTATTATAATGATGATAGTTTTTTCACTTTTAACTTTTTTAGTTGACATAGTTTTACTTATCAGATTTATTACATTAATAAAACAAATATTCTATAAAGA > CP028101/1228143‑1228602 | cATTATAAAAATAAAAATTATAATAAAGTTCATTATTTTCCCACTTTATATCAAGTTTAATACTTCCATTAGGATAACTTTTAAAAAAATCTGCTGGACTTACAAAAACATTCACACAAAATACAAAAAAAAAAGTCAAAATTAAAAAAATCTTTTTCATAAACTCCTCTCATATTTCTAAAATAATTTATTATAAAATCATCTGTTCTATTATA‑GTC‑‑T‑TTTTTTTTTTTTTTTT‑CAAGCACTGTTTGaa < 1:568350/250‑1 (MQ=255) gAATTATAATAAAGTTCATTATTTTCCCACTTTATATCAAGTTTAATACTTCCATTAGGATAACTTTTAAAAAAATCTGCTGGACTTGCAAAAACATTCACACAAAATACAAAAAATAAAGTCAAAATTAAAAAAATCTTTTTCATAAACTCCTCTCATATTTCTAAAATAATTTATTATAAAATCATCTGTTCTATTATA‑GTCGTT‑TTTTTTTTTTTTTTT‑‑CAATCACTGTTTGaaaa > 2:37220/1‑239 (MQ=255) gAATTATAATAAAGTTCATTATTTTCCCACTTTATATCAAGTTTAATACTTCCATTAGGATAACTTTTAAAAAAATCTGCTGGACTTGCAAAAACATTCACACAAAATACAAAAAAAAAAGTCAAAATTAAAAAAATCTTTTTCATAAACTCCTCTCATATTTCTAAAATAATTTATTATAAAATCATCTGTTCTATTATA‑GTCGTT‑TTTTTTTTTTTTTTT‑‑CAAGCACTGTTTGaaaa < 1:37220/239‑1 (MQ=255) ttATAATAAAGTTCATTATTTTCCCACTTTATATCAAGTTTAATACTTCCATTAGGATAACTTTTAAAAAAATCTGCTGGACTTGCAAAAACATTCACACAAAATACAAAAAATAAAGTCAAAATTAAAAAAATCTTTTTCATAATCTCCTCTCATATTACTAAAATAATTTATTATAAAATCATCTGTTCTATTATA‑GTCGTTGTTTTTTTTTTTTTTTT‑TAAACACTGTTTTAAAATATATTT‑aaaaaa > 1:719593/1‑251 (MQ=255) ttATAATAAAGTTCATTATTTTCCCACTTTATATCAAGTTTAATACTTCCATTAGGATAACTTTTAAAAAAATCTGCTGGACTTGCAAAAACATTCACACAAAATACAAAAAATAAAGTCAAAATTAAAAAAATCTTTTTCATAAACTCCTCTCATATTTCTAAAATAATTTATTATAAAATCATCTGTTCTATTATA‑GTCGTT‑TTTTTTTTTTTTTTTT‑CAAGCACTGTTTGaaaactgtcttttatata > 2:708373/1‑237 (MQ=255) ttATAATAAAGTTCATTATTTTCCCACTTTATATCAAGTTTAATACTTCCATTAGGATAACGTTTAAGAAAATCTGCTGGACTTGCAAAAACATTCACACAAAATACAAAAAATAAAGTCAAAATTAAAAAAATCTTTTTCATAAACTCCTCTCATATTTCTAAAATAATTTATTATAAAATCATCTGTTCTATTATA‑GTCGTT‑TTTTTTTTTTTTTTTT‑CAAGCACTGTTTGaaaa < 1:708373/237‑1 (MQ=255) ataataAAGTTCATTATTTTCCCACTTTATATCAAGTTTAATACTTCCATTAGGATAACTTTTAAAAAAATCTGCTGGACTTGCAAAAACATTCACACAAAATACAAAAAATAAAGTCAAAATTAAAAAAATCTTTTTCATAAACTCCTCTCATATTTCTAAAATAATTTATTATAAAATCATCTGTTCTATTATA‑GTCGTT‑TTTTTTTTTTTTTTTT‑CAAGCATTATTTGAAATTATATTT‑AAAAAatat > 2:749445/1‑251 (MQ=255) ataataAAGTTCATTATTTTCCCACTTTATATCAAGTTTAATACTTCCATTAGGATAACTTTTAAAAAAATCTGCTGGACTTGCAAAAACATTCACACAAAATACAAAAAATAAAGTCAAAATTAAAAAAATCTTTTTCATAAACTCCTCTCATATTTCTAAAATAATTTATTATAAAATCATCTGTTCTATTATA‑GTCGTT‑TTTTTTTTTTTTTTTT‑‑AAGCACTGTTTGaaaa > 2:92718/1‑234 (MQ=255) ataataAAGTTCATTATTTTCCCACTTTATATCAAGTTTAATACTTCCATTAGGATAACTTTTAAAAAAATCTGCTGGACTTGCAAAAACATTCACACAAAATACAAAAAATAAAGTCAAAATTAAAAAAATCTTTTTCATAAACTCCTCTCATATTTCTAAAATAATTTATTATAAAATCATCTGTTCTATTATA‑GTCGTT‑TTTTTTTTTTTTTTT‑‑CAAGCACTGTTTGaaaa < 1:92718/234‑1 (MQ=255) ataaAGTTCATTATTTTCCCACTTTATATCAAGTTTAATACTTCCATTAGGATAACTTTTAAAAAAATCTGCTGGACTTGCAAAAACATTCACACAAAATACAAAAAATAAAGTCAAAATTAAAAAAATCTTTTTCATAAACTCCTCTCATATTTCTAAAATAATTTATTATAAAATCATCTGTTCTATTATA‑GTCGTT‑TTTTTTTTTTTTTTT‑‑CAAGCACTGTTTGAAAATATATTT‑AAAAAatatat > 1:55111/1‑249 (MQ=255) attattTTCCCACTTTATATCAAGTTTAATACTTCCATTAGGATAACTTTTAAAAAAATCTGCTGGACTTGCAAAAACATTCACACAAAATACAAAAAATAAAGTCAAAATTAAAAAAATCTTTTTCATAAACTCCTCTCATATTTCTAAAATAATTTATTATAAAATCATCTGTTCTATTATA‑GTCGTT‑TTTTTTTTTTTTTT‑‑‑CAAGCACTGTTTGaaaa > 2:17922/1‑221 (MQ=255) attattTTCCCACTTTATATCAAGTTTAATACTTCCATTAGGATAACTTTTAAAAAAATCTGCTGGACTTGCAAAAACATTCACACAAAATACAAAAAATAAAGTCAAAATTAAAAAAATCTTTTTCATAAACTCCTCTCATATTTCTAAAATAATTTATTATAAAATCATCTGTTCTATTATA‑GTCGTT‑TTTTTTTTTTTTTT‑‑‑CAAGCACTGTTTGaaaa < 1:17922/221‑1 (MQ=255) attTTCCCACTTTATATCAAGTTTAATACTTCCATTAGGATAACTTTTAAAAAAATCTGCTGGACTTGCAAAAACATTCACACAAAATACAAAAAATAAAGTCAAAATTAAAAAAATCTTTTTCATAAACTCCTCTCATATTTCTAAAATAATTTATTATAAAATCATCTGTTCTATTATA‑GTCGTT‑TTTTTTTTTTTTTTTT‑CAAGCACTGTTTGAAAATATATTT‑AAAAAATATTTCTAAATTAAAAat > 2:236801/1‑251 (MQ=255) cACTTTATATCAAGTTTAATTCTTCCATTAGGATAACTTTTAAAAAAATCTGCTGGACTTGCAAAAACATTCACACAAAATACAAAAAATAAAATCAAAATTAAAAAAATCTTTTTCATAAACTCCTCTCATATTTCTAAAAAAATTTATTATAAAATCATCTTTTCTATTATA‑GTCGTT‑TTTTTTTTTTTTTTTT‑CAAGCACTGTTTGAAAATATATTT‑AAAAAATATATCTAAAATAAAAATTATTTTc < 1:236801/251‑1 (MQ=255) aCTTTATATCAAGTTTAATACTTCCATTAGGATAACTTTTAAAAAAATCTGCTGGACTTGCAAAAACATTCACACAAAATACAAAAAATAAAGTCAAAATTAAAAAAATCTTTTTCATAAACTCCTCTCATATTTCTAAAATAATTTATTATAAAATCATCTGTTCTATTATA‑GTCGTT‑TTTTTTTTTTTTT‑‑‑‑CAAGCACTGTTTGAAAATATATTT‑AAAAAATATATCTAAAATAAAAATTATTTTCTTAt > 1:48473/1‑251 (MQ=255) cTTTATATCAAGTTTAATTATTCCATTAGGATAACTTTTAAAAAAATCTGCTGGACTTTCAAAAACATTCAAACAAAATACAAAAAATAAAATCAAAAATAAAAAAATCTTTTTCATAAACTCCTCTCATATTTCTAAAATAATTTATTATAAAATCATCTGTTCTATTATA‑GTCGTT‑TTTTTTTTTTTTTTTT‑CAAGCACTGTTTGAAAat < 1:354243/212‑1 (MQ=255) cTTTATATCAAGTTTAATACTTCCATTAGGATAACTTTTAAAAAAATCTGCTGGACTTGCAAAAACATTCACACAAAATACAAAAAATAAAGTCAAAATTAAAAAAATCTTTTTCATAAACTCCTCTCATATTTCTAAAATAATTTATTATAAAATCATCTGTTCTATTATA‑GTCGTT‑TTTTTTTTTTTTTTTTTCAAGCACTTTTTGAAAATAAATTT‑AAAAAATATATTTAAAATAAAAATAAtttttt > 2:228230/1‑249 (MQ=255) gTTTAATACTTCCATTAGGATAACTTTTAAAAAAATCTGCTGGACTTGCAAAAACATTCACACAAAATACAAAAAATAAAGTCAAAATTAAAAAAATCTTTTTCATAAACTCCTCTCATATTTCTAAAATAATTTATTATAAAATCATCTGTTCTATTATA‑GTCGTT‑TTTTTTTTTTTTTTTT‑TAAGCAATTTTTGAAAAAATATTT‑AAAAAATATTTTTAAATTAAAAATTATTTTTTTaatataaaatt > 2:427486/1‑241 (MQ=255) gTTTAATACTTCCATTAGGATAACTTTTAAAAAAATCTGCTGGACTTGCAAAAACATTCACACAAAATACAAAAAATAAAGTCAAAATTAAAAAAATCTTTTTCATAAACTCCTCTCATATTTCTAAAATAATTTATTATAAAATCATCTGTTCTATTATA‑GTCGTT‑TTTTTTTTTTTTTTTT‑CAAGCACTGTTTGAAAATATATTT‑AAAAAATATATCTAAAATAAAAATTATTTTCTTATGCTATAAtt > 1:271861/1‑251 (MQ=255) ttAATACTTCTATTAGAATAAATTTTAAAAAAATCTTCTAGACTTGAAAAAACATTAACACAAAATACAAAAAATAAAGTCAAAATTAAAAAAATCTTTTTCATAAACTCCTCTCATATTTCTAAAATAATTTATTATAAAATCATCTGTTCTATTATA‑GTCGTT‑TTTTTTTTTTTTT‑‑‑‑CAAGCACTGTTTGAAAATATATTT‑AAAAAATATATCTAAAATAAAAATTATTTTCTTATGCTATAATTAAAAt < 2:466006/251‑1 (MQ=255) cTTCCATTAGGATAACTTTTAAAAAAATCTGCTGGACTTGCAAAAACATTCACACAAAATACAAAAAATAAAGTCAAAATTAAAAAAATCTTTTTCATAAACTCCTCTCATATTTCTAAAATAATTTATTATAAAATCATCTGTTCTATTATA‑GTCGTT‑TTTTTTTTTTTTTTT‑‑CAAGCACTGTTTGAAAATATATTT‑AAAAAATATATCTAAAATAAAAATTTTTTTTTTATTCTATAATTAAAATaaaa > 1:427804/1‑250 (MQ=255) ttCCATTAGGATAACTTTTAAAAAAATCTGCTGGACTTGCAAAAACATTCACACAAAATACAAAAAATAAAGTCAAAATTAAAAAAATCTTTTTCATAAACTCCTCTCATATTTCTAAAATAATTTATTATAAAATCATCTGTTCTATTATA‑GTCGTT‑TATTTTTTTTTATTTT‑CGAGCACTGTTTGAAAATATATTT‑AAAAAATATATCTATAATAAAAATTTTTTTTTTATGTTATAATTAAAATaaaa > 1:678953/1‑250 (MQ=255) tgtttAAGAAAATCTGCAAGAATTTCAAAAACATTCACAAAAAATACAAAAAATAAAATCAAAATTAAAAAAAACTTTTTCAAAAACTCCCCTCATATTTTTAAAATAATTTATTTTAAAATCATCTTTTCTATTAAA‑GTCTTT‑TTTTTTTTTTTTTTTT‑CAAGCACTGTTTGaaaa < 1:716858/175‑1 (MQ=255) cTTTTAAAAAAATCTGCTGGACTTGCAAAAACATTCACACAAAATACAAAAAATAAAGTCAAAATTAAAAAAATCTTTTTCATAAACTCCTCTCATATTTCTAAAATAATTTATTATAAAATCATCTGTTCTATTATA‑GTCGTT‑TTTTTTTTTTTTTTT‑‑CAAGCACTGTTTGAAAATATATTT‑AAAAAATATATCTAAAATAAAAATTATTTTTTTATGCTATAATTAAAATAAAGAATATTTTTGGTAAg > 2:222023/1‑251 (MQ=255) aattggtggaatttcAAAAACATTTCCACAAAATACAAAAAAAAAAGAAAAAATTAAAAAAAATTTTTTTATAAAATACATTTATATTTATAAAAAAATTTATAAAAAAAACAAATTTTTAAATATA‑AACATA‑TTTTTTTTTTTTTTTT‑CAAGCACTGTTTGAAAATATATTT‑AAAAAATATATCTAAAATAAAAATTATTTTCTTATGCTATAATTAAAATAAAGAATATTTTTGGAAAGGATTAAAAat < 1:28059/237‑1 (MQ=255) gACTTGCAAAAACATTCACACAAAATACAAAAAATAAAGTCAAAATTAAAAAAATCTTTTTCATAAACTCCTCTCATATTTCTAAAATAATTTATTATAAAAAAATCTGTTCTATTATA‑GTC‑AT‑TTTTTTTTTTTTTTTT‑CAAGCACTGTTTGAAAATATATTT‑AAAAAATATATCTAAAATAAAAATTATTTTCTTATGCTATAATTAAAATAAAGAATATTTTTGGAAAGGATTAAAAATATATTatg < 1:222023/250‑1 (MQ=255) aaaaaaTTCAAACAAAATAAAAAAAATAAAGTCAAAATTAAAAAAATATTTTTCATAAACTCTTCTCATATTTCTAAAATAATTTATTATAAAATAATCTGTTCTATTATA‑GTCGTT‑TTTTTTTTTTTTTTTT‑CAAGCACTGTTTGAAAATATATTT‑AAAAAATATATCTAAAATAAAAATTATTTTCTTATGCTATAATTAAAATAAAGAATATTTTTGGAAAGGATTAAAAATATATTATGAATGAAGt < 2:416591/251‑1 (MQ=255) acaaaaaaataaaaaaaaaaaaaataaaaTTAAAAAAATTTTTTTTATAATTTTCTTTTATATTTTTAAAATAATTTTTTAAAAAATAATCTTTTTTATTATAATTTTTT‑TTTTTTTTTTTTTTTT‑CAAGCACTGTTTGAAAATATATTT‑AAAAAATATATCTAAAATAAAAATTATTTTCTTATGCTATAATTAAAATAAAGAATATTTTTGGAAAGGATTAAAAATATATTATGAATGAAGTAAtattt < 2:719593/226‑1 (MQ=255) cacaAAATACAAAAAATAAAGTCAAAATTAAAAAAATCTTTTTCATAAACTCCTCTCATATTTCTAAAATAATTTATTATAAAATCATCTGTTCTATTATA‑GTCGTT‑TTTTTTTTTTTT‑‑‑‑‑CAAGCACTGTTTGAAAATATATTT‑AAAAAATATATCTAAAATAAAAATTATTTTCTTATGCTATAATTAAAATAAAGAATATTTTTGGAAAGGATTAAAAATATATTATGAATGAAGTAATATTTATTATaa > 1:917/1‑251 (MQ=255) aaaaaaaaCAAAAAATAAAATAAAAATTAAAAAAATTTTTTTCATAAACTCCTCTTATATTTCTAAAATAATTTATTATAAAATCATCTGTTCTATTATA‑GTCGTT‑TTTTTTTTTTTTTTT‑‑CAAGCACTGTTTGAAAATATATTT‑AAAAAATATATCTAAAATAAAAATTATTTTCTTATGCTATAATTAAAATAAAGAATATTTTTGGAAAGGATTAAAAATATATTATGAATGAAGTAATATTtattat < 1:116778/249‑1 (MQ=255) aaaTACAAAAAATAAAGTCAAAATTAAAAAAATCTTTTTCATAAACTCCTCTCATATTTCTAAAATAATTTATTATAAAATCATCTGTTCTATTATA‑GTCGTT‑TTTTTTTTTTTTTTTT‑CAAGCCCTTTTTGAAAATATATTT‑AAAAAATATATCTAAAATAAAAATTATTTTCTTTTGCTATAATTAAAATAAAGAATATTTTTGGGAAGGATTAAAAATATATTATGAATGAAGTAATATTTATTATaa > 1:325196/1‑251 (MQ=255) aaaaaaaaTAAAGTAAAAATTAAAAAAATCTTTTTTATAAACCCCTTTCATTTTTTAAAAATAATTTATTATAAAATTATCTTTTATATTATA‑GTTGTT‑TTTTTTTTTTTTTTT‑‑CAAGCACTGTTTGAAAATATATTT‑AAAAAATATATCTAAAATAAAAATTATTTTCTTATGCTATAATTAAAATAAAGAATATTTTTGGAAAGGATTAAAAATATATTATGAATGAAGTAATATTTATTATAatgatg < 2:427804/249‑1 (MQ=255) ctcctattatatTTCTAAAATAATTTATTATAAAATCATCTGTTCTATTATA‑GACGTT‑TTTTTTTTTTTTT‑‑‑‑CAAGCACTGTTTGAAAATATATTT‑AAAAAATATATCTAAAATAAAAATTATTTTCTTATGCTATAATTAAAATAAAGAATATTTTTGGAAAGGATTAAAAATATATTATGAATGAAGTAATATTTATTATAATGATGATAGTTTTTTCACTTTTAACTTTTTTAGTTGACATAGTTTTAc < 2:48473/243‑1 (MQ=255) tttAAAATAATTTATTTTAAAATATTCTGTTTTATTTTA‑GTTGTT‑TTTTTTTTTTTTTTTT‑CAAGCACTGTTTGAAAATATATTT‑AAAAAATATATCTAAAATAAAAATTATTTTCTTATGCTATAATTAAAATAAAGAATATTTTTGGAAAGGATTAAAAATATATTATGAATGAAGTAATATTTATTATAATGATGATAGTTTTTTCACTTTTAACTTTTTTAGTTGACATAGTTTTACTTATCAGAtt < 2:325196/249‑1 (MQ=255) ataAAATCATCTGTTCTATTATA‑GTCGTT‑TTTTTTTTTTTTTTTT‑CAAGCACTATTTGAAAAAATATTTTAAAAATTTTTTCTAAA‑TAAAAATTATTTTTTTAAGATATAATTAAAATAAATAATTTTTTTATAAAGGTTTTTAAATTTTTTTTTTATAAAGAAATATTTATTATAATATTTAAAGTTTTTTTAATTTTAACTTTTTTATTTTACTTAGTTTTATTTATTAAATTTTTTACATTAATaaaa > 2:832393/1‑251 (MQ=255) aaaaTCATCTGTTCTATTATA‑GTCGTT‑TTTTTTTTTTTTTT‑‑‑CAAGCACTGTTTGAAAATATATTT‑AAAAAATATATCTAAAATAAAAATTATTTTCTTATGCTATAATTAAAATAAAGAATATTTTTGGAAAGGATTAAAAATATATTATGAATGAAGTAatat > 1:344393/1‑164 (MQ=255) aaaaatcatatttattattataatattttttttttttttt‑CAAGCACTGTTTGAAAATATATTT‑AAAAAATATATCTAAAATAAAAATTATTTTCTTATGCTATAATTAAAATAAAGAATATTTTTGGAAAGGATTAAAAATATATTATGAATGAAGTAATATTTATTATAATGATGATAGTTTTTTCACTTTTAACTTTTTTAGTTGACATAGTTTTACTTATCAGATTTATTACATTAATAAAACAAat < 2:917/226‑1 (MQ=255) gTTCTATTATA‑GTCGTT‑TTTTTTTTTTTTTTT‑‑CAAGCACTGTTTGAAAATATATTT‑AAAAAATATATc < 1:101312/68‑1 (MQ=255) aatattgttttt‑tttttttttttttttt‑CAAGCACTGTTTGAAAATATATTT‑AAAAAATATATCTAAAATAAAAATTATTTTCTTATGCTATAATTAAAATAAAGAATATTTTTGGAAAGGATTAAAAATATATTATGAATGAAGTAATATTTATTATAATGATGATAGTTTTTTCACTTTTAACTTTTTTAGTTGACATAGTTTTACTTATCAGATTTATTACATTAATAAAACAAATATTCTATAAAGa < 2:678953/241‑1 (MQ=255) | CATTATAAAAATAAGAATTATAATAAAGTTCATTATTTTCCCACTTTATATCAAGTTTAATACTTCCATTAGGATAACTTTTAAAAAAATCTGCTGGACTTGCAAAAACATTCACACAAAATACAAAAAATAAAGTCAAAATTAAAAAAATCTTTTTCATAAACTCCTCTCATATTTCTAAAATAATTTATTATAAAATCATCTGTTCTATTATA‑GTCGTT‑TTTTTTTTTTTTTTTT‑CAAGCACTGTTTGAAAATATATTT‑AAAAAATATATCTAAAATAAAAATTATTTTCTTATGCTATAATTAAAATAAAGAATATTTTTGGAAAGGATTAAAAATATATTATGAATGAAGTAATATTTATTATAATGATGATAGTTTTTTCACTTTTAACTTTTTTAGTTGACATAGTTTTACTTATCAGATTTATTACATTAATAAAACAAATATTCTATAAAGA > CP028101/1228143‑1228602 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |