Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP028101	880,795	A→T	K1889N (AAA→AAT)	C7Y58_04335 →	autotransporter domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP028101	880,795	0	A	T	100.0%	147.9 / NA	45	K1889N (AAA→AAT)	C7Y58_04335	autotransporter domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (24/21);  total (24/21)

AGATAAAGAATTTAAATATCTAAGAGATGAATGGAGAACAGCTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAAGATATAGGAAAATCAAAAGAACATATGTTACAAGCAAAGGTAGGATTATTAAAATCAGTGCCATTTGATGATAACAATAGCTTGAATTGGACAATATCAGGA  >  CP028101/880555‑880885                                                                                                                                                                                                                                                 |                                                                                           aGATAAAGAATTTAAATATCTAAGAGATGAATGGAGAACAGCTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGaa                                                                                   >  2:422121/1‑250 (MQ=255)  gATAAAGAATTTAAATATCTAAGAGATGAATGGAGAACAGCTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAACa                                                                                 >  2:707373/1‑251 (MQ=255)                atatCTAAGAGATGAATGGAGAACAGCTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAACAAATGTTgcaagcaa                                                                   >  1:334700/1‑251 (MQ=255)                atatCTAAGAGATGAATGGAGAACAGCTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAACAAATGTTgcaagcaa                                                                   >  1:365157/1‑251 (MQ=255)                  atCTAAGAGATGAATGGAGAACAGCTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGGGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACCTGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAACAAATGTTGCTAGCAAAg                                                                 >  1:655540/1‑251 (MQ=11)                   tCTAAGAGATGAATGGAGAACAGCTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAACAAATGTTGCAAGCAAAgg                                                                >  2:315752/1‑251 (MQ=255)                   tCTAAGAGATGAATGGAGAACAGCTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAACAAATGTTGCAAGCAAAgg                                                                >  1:69262/1‑251 (MQ=255)                   tCTAAGAGATGAATGGAGAACAGCTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAACAAATGTTGCAAGCAAAgg                                                                >  2:541400/1‑251 (MQ=255)                          gaTGAATGGAGAACAGCTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAACAAATGTTGCAAGCAAAGGTAGGatt                                                         >  2:161111/1‑251 (MQ=255)                          gaTGAATGGAGAACAGCTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAACAAATGTTGCAAGCAAAGGTAGGatt                                                         >  1:330903/1‑251 (MQ=255)                           aTGAATGGAGAACAGCTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAACAAATGTTGCAAGCAAAGGTAGGatt                                                         <  2:315894/250‑1 (MQ=255)                             gAATGGAGAACAGCTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAACAAATGTTGCAAGCAAAGGTAGGattatt                                                      >  1:118083/1‑251 (MQ=255)                              aaTGGAGAACAGCTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTTATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAACAAATGTTGCAAGCAAAGGTAGGAttatta                                                     <  2:326725/251‑1 (MQ=255)                              aaTGGAGAACAGCTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAACAAATGTTGCAAGCAAAGGTAGGAttatta                                                     <  2:270424/251‑1 (MQ=255)                               aTGGAGAACAGCTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATGCAAAACAGATACAGCTGGAGTGATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAACAAATGTTGCAAGCAAAGGTAGGATTATTaa                                                    <  1:536106/251‑1 (MQ=14)                               aTGGAGAACAGCTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCACGGATATAGGAAATTCAAAAGAACAAATGTTGCAAGCAAAGGTAGGATTATTaa                                                    >  1:510779/1‑251 (MQ=255)                               aTGGAGAACAGCTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAACAAATGTTGCAAGCAAAGGTAGGATTATTaa                                                    >  2:654334/1‑251 (MQ=255)                                 ggAGAACAGCTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGCGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGATCAAGGATATAGGAAATTCAAAAGAACAAATGTTGCAAGCAAAGGTAGGATTATTaaaa                                                  >  2:637048/1‑251 (MQ=255)                                     aaCAGCTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTATAGGAGAATACAAAACAGATACAGCAGGAGTTATACACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAACAAATGTTGCAAGCAAAGGTAGGATTATTAAAATc                                                <  1:571004/249‑1 (MQ=255)                                     aaCAGCTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAACAAATGTTGCAAGCAAAGGTAGGATTATTAAAATc                                                <  1:161111/249‑1 (MQ=255)                                     aaCAGCTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAACAAATGTTGCAAGCAAAGGTAGGATTATTAAAATc                                                <  1:110031/249‑1 (MQ=255)                                     aaCAGCTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAACAAATGTTGCAAGCAAAGGTAGGATTATTAAAATc                                                <  2:69262/249‑1 (MQ=255)                                          cTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAACAAATGTTGCAAGCAAAGGTAGGatt                                                         <  1:37894/235‑1 (MQ=255)                                          cTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAACAAATGTTGCAAGCAAAGGTAGGatt                                                         >  2:37894/1‑235 (MQ=255)                                          cTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAACAAATGTTGCAAGCAAAGGTAGGATTATTAAAATCAGTGCCa                                         >  2:565891/1‑251 (MQ=255)                                            tCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAACAAATGTTGCAAGCAAAGGTAGGatt                                                         >  1:364634/1‑233 (MQ=255)                                            tCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAACAAATGTTGCAAGCAAAGGTAGGatt                                                         <  2:364634/233‑1 (MQ=255)                                                   aTTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACCGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGACATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAACAAATGTTGCAAGCAAAGGTAGGATTATTAAAATCAGTGCCATTTgatgat                                <  1:637048/251‑1 (MQ=255)                                                      cAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAACAAATGTTGCAAGCAAAGGTAGGATTATTAAAATCAGTGCCATTTGATGATAAc                             <  1:707373/251‑1 (MQ=255)                                                           aaaataaaaGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAACAAATGTTGCAAGCAAAGGTAGGATTATTAAAATCAGTGCCATTTGATGATAACAATAg                        <  2:94689/251‑1 (MQ=255)                                                            taataaaaGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAACAAATGTTGCAAGCAAAGGTAGGATTATTAAAATCAGTGCCATTTGATGATAACAATAg                        >  2:379378/2‑250 (MQ=255)                                                             aataaaaGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTACGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAACAAATGTTGCAAGCAAAGGTAGGATTATTAAAATCAGTGCCATTTGATGATAACAATAGCt                      <  1:654334/251‑1 (MQ=255)                                                              ataaaaGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATCGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAACAAATGTTGCAAGCAAAGGTAGGATTATTAAAATCAGTGCCATTTGATGATAACAATAGCtt                     <  1:565891/251‑1 (MQ=255)                                                                     cATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAACAAATGTTGCAAGCAAAGGTAGGATTATTAAAATCAGTGCCATTTGATGATAACAATAGCTTGAATTgg              >  1:270202/1‑251 (MQ=16)                                                                      aTTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGCATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAACAAATGTTGCAAGCAAAGGTAGGATTATTAAAATCAGTGCCAATTGATGATAACAATAGCTTGAATTGGa             >  2:315464/1‑251 (MQ=255)                                                                           gAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAACAAATGTTGCAAGCAAAGGTAGGATTATTAAAATCAGTGCCATTTGATGATAACAATAGCTTGAATTGGACaaaa        >  2:33491/1‑249 (MQ=255)                                                                            aaCTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAACAAATGTTGCAAGCAAAGGTAGGATTATTAAAATCAGTGCCATTTGATGATAACAATAGCTTGAATTGGACAatat       >  1:449642/1‑251 (MQ=12)                                                                            aaCTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAACAAATGTTGCAAGCAAAGGTAGGATTATTAAAATCAGTGCCATTTGATGATAACAATAGCTTGAATTGGACAatat       >  1:361939/1‑251 (MQ=12)                                                                             aCTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAACAAATGTTGCAAGCAAAGGTAGGATTATTAAAATCAGTGCCATTTGATGATAACAATAGCTTGAATTGGACAATATc      <  2:261946/251‑1 (MQ=12)                                                                                agagGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAACAAATGTTGCAAGCAAAGGTAGGATTATTAAAATCAGTGCCATTTGATGATAACAATAGCTTGAATTGGAc            <  1:401561/242‑1 (MQ=9)                                                                                agagGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAACAAATGATGCAAGCAAAGGTAGGATTATTAAAATCAGTGCCATTTGATGATAACAATAGCTTGAATTGGAc            >  2:401561/1‑242 (MQ=9)                                                                                  aggagAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAACAAATGTTGCAAGCAAAGGTAGGATTATTAAAATCAGTGCCATTTGATGATAACAATAGCTTGAATTGGACAATATCAgg   <  1:422121/249‑1 (MQ=2)                                                                                  aggagAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAACAAATGTTGCAAGCAAAGGTAGGATTATTAAAATCAGTGCCATTTGATGATAACAATAGCTTGAATTGGACAATATCAgg   <  2:90810/249‑1 (MQ=2)                                                                                  aggagAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAACAAATGTTGCAAGCAAAGGTAGGATTATTAAAATCAGTGCCATTTGATGATAACAATAGCTTGAATTGGACAATATCAGga  <  2:237929/250‑1 (MQ=2)                                                                                  aggagAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAACAAATGTTGCAAGCAAAGGTAGGATTATTAAAATCAGTGCCATTTGATGATAACAATAGCTTGAATTGGACAATATCAGga  <  1:121896/250‑1 (MQ=2)                                                                                                                                                                                                                                                 |                                                                                           AGATAAAGAATTTAAATATCTAAGAGATGAATGGAGAACAGCTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAAGATATAGGAAAATCAAAAGAACATATGTTACAAGCAAAGGTAGGATTATTAAAATCAGTGCCATTTGATGATAACAATAGCTTGAATTGGACAATATCAGGA  >  CP028101/880555‑880885

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑