Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP028101	761,017	A→G	I464M (ATA→ATG)	C7Y58_03755 →	hemolysin activator protein precursor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP028101	761,017	0	A	G	100.0%	68.1 / NA	19	I464M (ATA→ATG)	C7Y58_03755	hemolysin activator protein precursor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/9);  total (10/9)

TGGAGTAGGAAGTGTTGGTGGTTATCATAGAACTGGAAATATACAAGGTGATAAAGCAATAGAAATAGAAAATGAATTATCATATAGAGTGTTAGACTCAGAAAAATTTGGAAAAATAACTCCTTATTTAAGTTATTCATATGGAAAAGTAAGAAATAACAAAAATAATTCAAAATATAGAAAAGGATATATGTCAGGTGCTATATTAGGTTTAAGATATAATATGAAATATTTAGATTTAGATGTAGCTTATGCAAAACCTTTAGCTCGTTCAAATTACTTAAAACCTAAGAATAGAGA  >  CP028101/760957‑761256                                                             |                                                                                                                                                                                                                                                tGGAGTAGGAAGTGTTGGTGGTTATCATAGAACTGGAAATATACAAGGTGATAAAGCAATGGAAATAGAAAATGAATTATCATATAGAGTGTTAGACTCAGAAAAGTTTGGAAAAATAACTCCTTATTTAAGTTATTCATATGGAAAAGTAAGAAATAACAAAAATAATTCAAAATATAGAAAAGGATATATGTCAGGTGCTATATTAGGTTTAAGATATAATATGAAATATTTAGATTTAGATGTAGCt                                                    <  1:269326/250‑1 (MQ=37)   gAGTAGGAAGTGTTGGTGGTTATCATAGAACTGGAAATATACAAGGTGATAAAGCAATGGAAATAGAAAATGAATTATCATATAGAGTGTTAGACTCAGAAAAGTTTGGAAAAATAACTCCTTATTTAAGTTATTCATATGGAAAAGTAAGAAATAACAAAAATAATTCAAAATATAGAAAAGGATATATGTCAGGTGCTATATTAGGTTTAAGATATAATATGAAATATttagatttagat                                                          >  1:293235/1‑242 (MQ=21)   gAGTAGGAAGTGTTGGTGGTTATCATAGAACTGGAAATATACAAGGTGATAAAGCAATGGAAATAGAAAATGAATTATCATATAGAGTGTTAGACTCAGAAAAGTTTGGAAAAATAACTCCTTATTTAAGTTATTCATATGGAAAAGTAAGAAATAACAAAAATAATTCAAAATATAGAAAAGGATATATGTCAGGTGCTATATTAGGTTTAAGATATAATATGAAATATttagatttagat                                                          <  2:293235/242‑1 (MQ=21)     gTAGGAAGTGTTGGTGGTTATCATAGAACTGGAAATATACAAGGTGATAAAGCAATGGAAATAGAAAATGAATTATCATATAGAGTGTTAGACTCAGAAAAGTTTGGAAAAATAACTCCTTATTTAAGTTATTCATATGGAAAAGTAAGAAATAACAAAAATAATTCAAAATATAGAAAAGGATATATGTCAGGTGCTATATTAGGTTTAAGATATAATATGAAATATTTAGATTTAGATGTAGCTTATg                                                >  2:132044/1‑250 (MQ=255)            gtgtTGGTGGTTATCATAGAACTGGAAATATACAAGGTGATAAAGCAATGGAAATAGAAAATGAATTATCATATAGAGTGTTAGACTCAGAAAAGTTTGGAAAAATAACTCCTTATTTAAGTTATTCATATGGAAAAGTAAGAAATAACAAAAATAATTCAAAATATAGAAAAGGATATATGTCAGGTGCTATATTAGGTTTAAGATATAATATGAAATATTTAGATTTAGATGTAGCTTATGCAAAAc                                          >  1:463422/1‑249 (MQ=255)                 ggtggtTATCATAGAACTGGAAATATACAAGGTGATAAAGCAATGGAAATAGAAAATGAATTATCATATAGAGTGTTAGACTCAGAAAAGTTTGGAAAAATAACTCCTTATTTAAGTTATTCATATGGAAAAGTAAGAAATAACAAAAATAATTCAAAATATAGAAAAGGATATATGTCAGGTGCTATATTAGGTTTAAGATATAATATGAAATATTTAGATTTAGATGTAGCTTATGCAAAACCTTTAg                                    >  1:190185/1‑250 (MQ=255)                   tggtTATCATAGAACTGGAAATATACAAGGTGATAAAGCAATGGAAATAGAAAATGAATTATCATATAGAGTGTTAGACTCAGAAAAGTTTGGAAAAATAACTCCTTATTTAAGTTATTCATATGGAAAAGTAAGAAATAACAAAAATAATTCAAAATATAGAAAAGGATATATGTCAGGTGCTATATTAGGTTTAAGATATAATATGAAATATTTAGATTTAGATGTAGCTTATGCAAAACCTTTAGCt                                  <  1:679675/250‑1 (MQ=255)                         tCATAGAACTGGAAATATACAAGGTGATAAAGCAATGGAAATAGAAAATGAATTATCATATAGAGTGTTAGACTCAGAAAAGTTTGGAAAAATAACTCCTTATTTAAGTTATTCATATGGAAAAGTAAGAAATAACAAAAATAATTCAGAATATAGAAAAGGATATATGTCAGGTGCTATATTAGCTTTAAGATATAATATGAACTATTTAGATTTAGATGTAGCTTATGCAAAACCTTTAGCTCGTTCaa                           >  1:602606/1‑251 (MQ=255)                          cATAGAACTGGAAATATACAAGGTGATAAAGCAATGGAAATAGAAAATGAATTATCATATAGAGTGTTAGACTCAGAAAAGTTTGGAAAAATAACTCCTTATTTAAGTTATTCATATGGAAAAGTAAGAAATAACAAAAATAATTCAAAATATAGGAAAGGATATATGTCAGGTGCTATATTAGGTTTAAGATATAATATGAAATATTTAGATTTAGATGTAGCTTATGCAAAACCTTTAGCTTGTTCaaa                          <  1:698477/251‑1 (MQ=255)                                   ggAAATATACAAGGTGATAAAGCAATGGAAATAGAAAATGAATTATCATATAGAGTGTTAGACTCAGAAAAGTTTGGAAAAATAACTCCTTATTTAAGTTATTCATATGGAAAAGTAAGAAATAACAAAAATAATTCAAAATATAGAAAAGGATATATGTCAGGTGCTATATTAGGTTTAAGATATAATATAAAATAGTTAGATTTAGATGTAGCTTATGCAAAACCTTTAGCTCGTTCAAATTACTTaaa                 >  1:502284/1‑251 (MQ=255)                                    gAAATATACAAGGTGATAAAGCAATGGAAATAGAAAATGAATTATCATATAGAGTGTTAGACTCAGAAAAGTTTGGAAAAATAACTCCTTATTTAAGTTATTCATATGGAAAAGTAAGAAATAACAAAAATAATTCAAAATATAGAAAAGGATATATGTCAGGTGCTATATTAGGTTTAAGATATAATATGAAATATTTAGATTTAGATGTAGCTTATGCAAAACCTTTAGCTCGTTCAAATTACTTaaaa                >  2:567565/1‑251 (MQ=255)                                     aaaTATACAAGGTGATAAAGCAATGGAAATAGAAAATGAATTATCATATAGAGTGTTAGACTCAGAAAAGTTTGGAAAAATAACTCCTTATTTAAGTTATTCATATGGAAAAGTAAGAAATAACAAAAATAATTCAAAATATAGAAAAGGATATATGTCAGGTGCTATATTAGGTTTAAGATATAATATGAAATATTTAGATTTAGATGTAGCTTATGCAAAACCTTTAGCTCGTTCAAATTACTTAAAAc               <  1:132044/251‑1 (MQ=255)                                     aaaTATACAAGGTGATAAAGCAATGGAAATAGAAAATGAATTATCATATAGAGTGTTAGACTCAGAAAAGTTTGGAAAAATAACTCCTTATTTAAGTTATTCATATGAAAAAGTAAGAAATAACAAAAATAATTCAAAATATAGAAAAGGATATATGTCAGGGGCTATATTATGTTTAAGATATAATATGAAATATTTAGATTTAGATGTAGCTTATGCAAAACCTTTAGCTCGTTCAAATTACTTAAAAc               <  2:707728/251‑1 (MQ=255)                                        tataCAAGGTGATAAAGCAATGGAAATAGAAAATGAATTATCATATAGAGTGTTAGACTCAGAAAAGTTTGGAAAAATAACTCCTTATTTAAGTTATTCATATGGAAAAGTAAGAAATAACAAAAATAATTCAAAATATAGAAAAGGATATATGTCAGGTGCTATATTAGGTTTAAGATATAATATGAAATATTTAGATTTAGATGTAGCTTATGCAAAACCTTTAGCTCGTTCAAATTACTTAAAACCTa            >  2:260666/1‑251 (MQ=255)                                         ataCAAGGTGATAAAGCAATGGAAATAGAAAATGAATTATCATATAGAGTGTTAGACTCAGAAAAGTTTGGAAAAATAACTCCTTATTTAAGTTATTCATATGGAAAAGTAAGAAATAACAAAAATAATTCAAAATATAGAAAAGGATATATGTCAGGTGCTATATTAGGTTTAAGATATAATATGAAATATTTAGATTTAGATGTAGCTTATGCAAAACCTTTAGCTCGTTCAAATTACTTAAAACCTaa           >  2:461198/1‑251 (MQ=255)                                          taCAAGGTGATAAAGCAATGGAAATAGAAAATGAATTATCATATAGAGTGTTAGACTCAGAAAAGTTTGGAAAAATAACTCCTTATTTAAGTTATTCATATGGAAAAGTAAGAAATAACAAAAATAATTCAAAATATAGAAAAGGATATATGTCAGGTGCTATATTAGGTTTAAGATATAATATGAAATATTTAGATTTAGATGTAGCTTATGCAAAACCTTTAGCTCGTTCAAATTACTTAAAACCTAAg          <  2:302586/251‑1 (MQ=255)                                             aaGGTGATAAAGCAATGGAAATAGAAAATGAATTATCATATAGAGTGTTAGACTCAGAAAAGTTTGGAAAAATAACTCCTTATTTAAGTTATTCATATGGAAAAGTAAGAAATAACAAAAATAATTCAAAATATAGAAAAGGATATATGTCAGGTGCTATATTAGGTTTAAGATATAATATGAAATATTTAGATTTAGATGTAGCTTATGCAAAACCTTTAGCTCGTTCAAATTACTTAAAACCTAAGAAt       <  1:341969/251‑1 (MQ=255)                                                gTGATAAAGCAATGGAAATAGAAAATGAATTATCATATAGAGTGTTAGACTCAGAAAAGTTTGGAAAAATAACTCCTTATTTAAGTTATTCATATGGAAAAGTAAGAAATAACAAAAATAATTCAAAATATAGAAAAGGATATATGTCAGGTGCTATATTAGGTTTAAGATATAATATGAAATATTTAGATTTAGATGTAGCTTATGCAAAACCTTTAGCTCGTTCAAATTACTTAAAACCTAAGAATaga    >  1:362884/1‑251 (MQ=255)                                                   aTAAAGCAATGGAAATAGAAAATGAATTATCATATAGAGTGTTAGACTCAGAAAAGTTTGGGAAAATAACTCCTTATTTAAGTTATTCATATGGAAAAGTAAGAAATAACAAAAATAATTCAAAATATAGAAAAGGATATATGTCAGGTGCTATATTAGGTTTAAGATATAATATGAAATATTTAGATTTAGATGTAGCTTATGCAAAACCTTTAGCTCGTTCAAATTACTTAAAACCTAAGAATAgaga  <  2:350425/250‑1 (MQ=255)                                                             |                                                                                                                                                                                                                                                TGGAGTAGGAAGTGTTGGTGGTTATCATAGAACTGGAAATATACAAGGTGATAAAGCAATAGAAATAGAAAATGAATTATCATATAGAGTGTTAGACTCAGAAAAATTTGGAAAAATAACTCCTTATTTAAGTTATTCATATGGAAAAGTAAGAAATAACAAAAATAATTCAAAATATAGAAAAGGATATATGTCAGGTGCTATATTAGGTTTAAGATATAATATGAAATATTTAGATTTAGATGTAGCTTATGCAAAACCTTTAGCTCGTTCAAATTACTTAAAACCTAAGAATAGAGA  >  CP028101/760957‑761256

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑