Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP028101	880,807	T→A	H1893Q (CAT→CAA)	C7Y58_04335 →	autotransporter domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP028101	880,807	0	T	A	100.0%	143.0 / NA	43	H1893Q (CAT→CAA)	C7Y58_04335	autotransporter domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (22/21);  total (22/21)

ATATCTAAGAGATGAATGGAGAACAGCTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAAGATATAGGAAAATCAAAAGAACATATGTTACAAGCAAAGGTAGGATTATTAAAATCAGTGCCATTTGATGATAACAATAGCTTGAATTGGACAATATCAGGA  >  CP028101/880570‑880885                                                                                                                                                                                                                                              |                                                                               atatCTAAGAGATGAATGGAGAACAGCTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAACAAATGTTgcaagcaa                                                                   >  1:334700/1‑251 (MQ=255) atatCTAAGAGATGAATGGAGAACAGCTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAACAAATGTTgcaagcaa                                                                   >  1:365157/1‑251 (MQ=255)   atCTAAGAGATGAATGGAGAACAGCTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGGGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACCTGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAACAAATGTTGCTAGCAAAg                                                                 >  1:655540/1‑251 (MQ=11)    tCTAAGAGATGAATGGAGAACAGCTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAACAAATGTTGCAAGCAAAgg                                                                >  2:541400/1‑251 (MQ=255)    tCTAAGAGATGAATGGAGAACAGCTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAACAAATGTTGCAAGCAAAgg                                                                >  2:315752/1‑251 (MQ=255)    tCTAAGAGATGAATGGAGAACAGCTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAACAAATGTTGCAAGCAAAgg                                                                >  1:69262/1‑251 (MQ=255)           gaTGAATGGAGAACAGCTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAACAAATGTTGCAAGCAAAGGTAGGatt                                                         >  2:161111/1‑251 (MQ=255)           gaTGAATGGAGAACAGCTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAACAAATGTTGCAAGCAAAGGTAGGatt                                                         >  1:330903/1‑251 (MQ=255)            aTGAATGGAGAACAGCTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAACAAATGTTGCAAGCAAAGGTAGGatt                                                         <  2:315894/250‑1 (MQ=255)              gAATGGAGAACAGCTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAACAAATGTTGCAAGCAAAGGTAGGattatt                                                      >  1:118083/1‑251 (MQ=255)               aaTGGAGAACAGCTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTTATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAACAAATGTTGCAAGCAAAGGTAGGAttatta                                                     <  2:326725/251‑1 (MQ=255)               aaTGGAGAACAGCTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAACAAATGTTGCAAGCAAAGGTAGGAttatta                                                     <  2:270424/251‑1 (MQ=255)                aTGGAGAACAGCTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATGCAAAACAGATACAGCTGGAGTGATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAACAAATGTTGCAAGCAAAGGTAGGATTATTaa                                                    <  1:536106/251‑1 (MQ=14)                aTGGAGAACAGCTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCACGGATATAGGAAATTCAAAAGAACAAATGTTGCAAGCAAAGGTAGGATTATTaa                                                    >  1:510779/1‑251 (MQ=255)                aTGGAGAACAGCTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAACAAATGTTGCAAGCAAAGGTAGGATTATTaa                                                    >  2:654334/1‑251 (MQ=255)                  ggAGAACAGCTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGCGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGATCAAGGATATAGGAAATTCAAAAGAACAAATGTTGCAAGCAAAGGTAGGATTATTaaaa                                                  >  2:637048/1‑251 (MQ=255)                      aaCAGCTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTATAGGAGAATACAAAACAGATACAGCAGGAGTTATACACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAACAAATGTTGCAAGCAAAGGTAGGATTATTAAAATc                                                <  1:571004/249‑1 (MQ=255)                      aaCAGCTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAACAAATGTTGCAAGCAAAGGTAGGATTATTAAAATc                                                <  1:161111/249‑1 (MQ=255)                      aaCAGCTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAACAAATGTTGCAAGCAAAGGTAGGATTATTAAAATc                                                <  2:69262/249‑1 (MQ=255)                      aaCAGCTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAACAAATGTTGCAAGCAAAGGTAGGATTATTAAAATc                                                <  1:110031/249‑1 (MQ=255)                           cTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAACAAATGTTGCAAGCAAAGGTAGGatt                                                         <  1:37894/235‑1 (MQ=255)                           cTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAACAAATGTTGCAAGCAAAGGTAGGatt                                                         >  2:37894/1‑235 (MQ=255)                           cTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAACAAATGTTGCAAGCAAAGGTAGGATTATTAAAATCAGTGCCa                                         >  2:565891/1‑251 (MQ=255)                             tCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAACAAATGTTGCAAGCAAAGGTAGGatt                                                         >  1:364634/1‑233 (MQ=255)                             tCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAACAAATGTTGCAAGCAAAGGTAGGatt                                                         <  2:364634/233‑1 (MQ=255)                                    aTTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACCGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGACATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAACAAATGTTGCAAGCAAAGGTAGGATTATTAAAATCAGTGCCATTTgatgat                                <  1:637048/251‑1 (MQ=255)                                       cAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAACAAATGTTGCAAGCAAAGGTAGGATTATTAAAATCAGTGCCATTTGATGATAAc                             <  1:707373/251‑1 (MQ=255)                                            aaaataaaaGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAACAAATGTTGCAAGCAAAGGTAGGATTATTAAAATCAGTGCCATTTGATGATAACAATAg                        <  2:94689/251‑1 (MQ=255)                                             taataaaaGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAACAAATGTTGCAAGCAAAGGTAGGATTATTAAAATCAGTGCCATTTGATGATAACAATAg                        >  2:379378/2‑250 (MQ=255)                                              aataaaaGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTACGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAACAAATGTTGCAAGCAAAGGTAGGATTATTAAAATCAGTGCCATTTGATGATAACAATAGCt                      <  1:654334/251‑1 (MQ=255)                                               ataaaaGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATCGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAACAAATGTTGCAAGCAAAGGTAGGATTATTAAAATCAGTGCCATTTGATGATAACAATAGCtt                     <  1:565891/251‑1 (MQ=255)                                                      cATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAACAAATGTTGCAAGCAAAGGTAGGATTATTAAAATCAGTGCCATTTGATGATAACAATAGCTTGAATTgg              >  1:270202/1‑251 (MQ=16)                                                       aTTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGCATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAACAAATGTTGCAAGCAAAGGTAGGATTATTAAAATCAGTGCCAATTGATGATAACAATAGCTTGAATTGGa             >  2:315464/1‑251 (MQ=255)                                                            gAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAACAAATGTTGCAAGCAAAGGTAGGATTATTAAAATCAGTGCCATTTGATGATAACAATAGCTTGAATTGGACaaaa        >  2:33491/1‑249 (MQ=255)                                                             aaCTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAACAAATGTTGCAAGCAAAGGTAGGATTATTAAAATCAGTGCCATTTGATGATAACAATAGCTTGAATTGGACAatat       >  1:361939/1‑251 (MQ=12)                                                             aaCTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAACAAATGTTGCAAGCAAAGGTAGGATTATTAAAATCAGTGCCATTTGATGATAACAATAGCTTGAATTGGACAatat       >  1:449642/1‑251 (MQ=12)                                                              aCTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAACAAATGTTGCAAGCAAAGGTAGGATTATTAAAATCAGTGCCATTTGATGATAACAATAGCTTGAATTGGACAATATc      <  2:261946/251‑1 (MQ=12)                                                                 agagGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAACAAATGTTGCAAGCAAAGGTAGGATTATTAAAATCAGTGCCATTTGATGATAACAATAGCTTGAATTGGAc            <  1:401561/242‑1 (MQ=9)                                                                 agagGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAACAAATGATGCAAGCAAAGGTAGGATTATTAAAATCAGTGCCATTTGATGATAACAATAGCTTGAATTGGAc            >  2:401561/1‑242 (MQ=9)                                                                   aggagAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAACAAATGTTGCAAGCAAAGGTAGGATTATTAAAATCAGTGCCATTTGATGATAACAATAGCTTGAATTGGACAATATCAgg   <  1:422121/249‑1 (MQ=2)                                                                   aggagAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAACAAATGTTGCAAGCAAAGGTAGGATTATTAAAATCAGTGCCATTTGATGATAACAATAGCTTGAATTGGACAATATCAgg   <  2:90810/249‑1 (MQ=2)                                                                   aggagAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAACAAATGTTGCAAGCAAAGGTAGGATTATTAAAATCAGTGCCATTTGATGATAACAATAGCTTGAATTGGACAATATCAGga  <  1:121896/250‑1 (MQ=2)                                                                   aggagAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAGGATATAGGAAATTCAAAAGAACAAATGTTGCAAGCAAAGGTAGGATTATTAAAATCAGTGCCATTTGATGATAACAATAGCTTGAATTGGACAATATCAGga  <  2:237929/250‑1 (MQ=2)                                                                                                                                                                                                                                              |                                                                               ATATCTAAGAGATGAATGGAGAACAGCTTCTAAAGATTCAAATAAAATAAAAGCATTTGGAACTAGAGGAGAATACAAAACAGATACAGCAGGAGTTATAGACTACAAGAATCATGCTTATGGAGTAGCTTATGTACATGAAAATGAAGATATTAAGCTAGGAAGAGGTATTGGATGGTACACAGGTATAGTTCATAATACATTTAAGTTCAAAGATATAGGAAAATCAAAAGAACATATGTTACAAGCAAAGGTAGGATTATTAAAATCAGTGCCATTTGATGATAACAATAGCTTGAATTGGACAATATCAGGA  >  CP028101/880570‑880885

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑