Predicted mutation
evidence seq id position mutation annotation gene description
RA CP028101 516,032 G→A G86D (GGT→GAT)  C7Y58_02640 → transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP028101516,0320GA99.8% 2456.0 / NA 635G86D (GGT→GAT) C7Y58_02640transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (317/317);  total (317/318)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 635 total aligned reads displayed.

TCATCCAACAGTAGATACAATATATAAAGCACTTTGTACAGAAATACCAACTTTATCTAAAACAACAGTGTACAATACTTTAAATTTATTTGTAGAGAAGAAGTTAGTTTATGTTATAGTTATAGAAGAAAATGAAACAAGATATGGTTTATTGACTCATACTCATGGACATTTTAAATGTACTTGTTGTGGAGCTTTATTTGATGTAGAATTAAATATTGATTATAGCAAAAGTCAAGAATTATTAGGCTGTGATATTGAAGAGAAACATATATATTTTAAAGGCATATGT  >  CP028101/515886‑516177
                                                                                                                                                  |                                                                                                                                                 
 catcCAACAGTAGATACAATATATAAAGCACTTTGTACAGAAATACCAACTTTATCTAAAACAACAGTGTACAATACTTTAAATTTATTTGTAGAGAAGAAGTTAGTTTATGTTATAGTTATAGAAGAAAATGAAACAAGATATGATTTa                                                                                                                                               >  2:1073298/1‑150 (MQ=255)
  atcCAACAGTAGATACAATATATAAAGCACTTTGTACAGAAATACCAACTTTATCTAAAACAACAGTGTACAATACTTTAAATTTATTTGTAGAGAAGAAGTTAGTTTATGTTATAGTTATAGAAGAAAATGAAACAAGATATGATTTAt                                                                                                                                              >  1:3300242/1‑150 (MQ=255)
     cAACAGTAGATACAATATATAAAGCACTTTGTACAGAAATACCAACTTTATCTAAAACAACAGTGTACAATACTTTAAATTTATTTGTAGAGAAGAAGTTAGTTTATGTTATAGTTATAGAAGAAAATGAAACAAGATATGATTTATTGa                                                                                                                                           >  1:1682487/1‑150 (MQ=255)
      aaCAGTAGATACAATATATAAAGCACTTTGTACAGAAATACCAACTTTATCTAAAACAACAGTGTACAATACTTTAAATTTATTTGTAGAGAAGAAGTTAGTTTATGTTATAGTTATAGAAGAAAATGAAACAAGATATGATTTATTGac                                                                                                                                          >  2:2894322/1‑150 (MQ=255)
      aaCAGTAGATACAATATATAAAGCACTTTGTACAGAAATACCAACTTTATCTAAAACAACAGTGTACAATACTTTAAATTTATTTGTAGAGAAGAAGTTAGTTTATGTTATAGTTATAGAAGAAAATGAAACAAGATATGATTTATTGac                                                                                                                                          <  2:3442652/150‑1 (MQ=255)
      aaCAGTAGATACAATATATAAAGCACTTTGTACAGAAATACCAACTTTATCTAAAACAACAGTGTACAATACTTTAAATTTATTTGTAGAGAAGAAGTTAGTTTATGTTATAGTTATAGAAGAAAATGAAACAAGATATGATTTATTGac                                                                                                                                          <  2:1348979/150‑1 (MQ=255)
          gTAGATACAATATATAAAGCACTTTGTACAGAAATACCAACTTTATCTAAAACAACAGTGTACAATACTTTAAATTTATTTGTAGAGAAGAAGTTAGTTTATGTTATAGTTATAGAAGAAAATGAAACAAGATATGATTTATTGactcat                                                                                                                                      >  2:2478286/1‑150 (MQ=255)
                  aaTATATAAAGCACTTTGTACAGAAATACCAACTTTATCTAAAACAACAGTGTACAATACTTTAAATTTATTTGTAGAGAAGAAGTTAGTTTATGTTATAGTTATAGAAGAAAATGAAACAAGATATGATTTATTGACTCATACTCATgg                                                                                                                              >  2:189863/1‑150 (MQ=255)
                  aaTATATAAAGCACTTTGTACAGAAATACCAACTTTATCTAAAACAACAGTGTACAATACTTTAAATTTATTTGTAGAGAAGAAGTTAGTTTATGTTATAGTTATAGAAGAAAATGAAACAAGATATGATTTATTGACTCATACTCATgg                                                                                                                              <  1:3351877/150‑1 (MQ=255)
                     atataAAGCACTTTGTACAGAAATACCAACTTTATCTAAAACAACAGTGTACAATACTTTAAATTTATTTGTAGAGAAGAAGTTAGTTTATGTTATAGTTATAGAAGAAAATGAAACAAGATATGATTTATTGACTCATACTCATGGACa                                                                                                                           >  1:2933960/1‑150 (MQ=255)
                      tataAAGCACTTTGTACAGAAATACCAACTTTATCTAAAACAACAGTGTACAATACTTTAAATTTATTTGTAGAGAAGAAGTTAGTTTATGTTATAGTTATAGAAGAAAATGAAACAAGATATGATTTATTGACTCATACTCATGGACAt                                                                                                                          >  2:3223306/1‑150 (MQ=255)
                         aaaGCACTTTGTACAGAAATACCAACTTTATCTAAAACAACAGTGTACAATACTTTAAATTTATTTGTAGAGAAGAAGTTAGTTTATGTTATAGTTATAGAAGAAAATGAAACAAGATATGATTTATTGACTCATACTCATGGACAtttt                                                                                                                       >  1:2147099/1‑150 (MQ=255)
                          aaGCACTTTGTACAGAAATACCAACTTTATCTAAAACAACATTGTACAATACTTTAAATTTATTTGTAGAGAAGAAGTTAGTTTATGTTATAGTTATAGAAGAAAATGAAACAAGATATGATTTACTGACTCATACTCATGGACATTTTa                                                                                                                      <  2:2674306/150‑1 (MQ=255)
                           aGCACTTTGTACAGAAATACCAACTTTATCTAAAACAACAGTGTACAATACTTTAAATTTATTTGTAGAGAAGAAGTTAGTTTATGTTATAGTTATAGAAGAAAATGAAACAAGATATGATTTATTGACTCATACTCATGGACATTTTaa                                                                                                                     >  2:2827116/1‑150 (MQ=255)
                           aGCACTTTGTACAGAAATACCAACTTTATCTAAAACAACAGTGTACAATACTTTAAATTTATTTGTAGAGAAGAAGTTAGTTTATGTTATAGTTATAGAAGAAAATGAAACAAGATATGATTTATTGACTCATACTCATGGACATTTTaa                                                                                                                     >  1:362608/1‑150 (MQ=255)
                               cTTTGTACAGAAATACCAACTTTATCTAAAACAACAGTGTACAATACTTTAAATTTATTTGTAGAGAAGAAGTTAGTTTATGTTATAGTTATAGAAGAAAATGAAACAAGATATGATTTATTGACTCATACTCATGGACATTTTAAATGt                                                                                                                 >  1:723682/1‑150 (MQ=255)
                                   gTACAGAAATACCAACTTTATCTAAAACAACAGTGTACAATACTTTAAATTTATTTGTAGAGAAGAAGTTAGTTTATGTTATAGTTATAGAAGAAAATGAAACAAGATATGATTTATTGACTCATACTCATGGACATTTTAAATGTACtt                                                                                                             >  1:896965/1‑150 (MQ=255)
                                    tACAGAAATACCAACTTTATCTAAAACAACAGTGTACAATACTTTAAATTTATTTGTAGAGAAGAAGTTAGTTTATGTTATAGTTATAGAAGAAAATGAAACAAGATATGATTTATTGACTCATACTCATGGACATTTTAAATGTACttg                                                                                                            <  1:2342220/150‑1 (MQ=255)
                                     aCAGAAATACCAACTTTATCTAAAACAACAGTGTACAATACTTTAAATTTATTTGTAGAGAAGAAGTTAGTTTATGTTATAGTTATAGAAGAAAATGAAACAAGATATGATTTATTGACTCATACTCATGGACATTTTAAATGTACttgt                                                                                                           >  1:1341200/1‑150 (MQ=255)
                                     aCAGAAATACCAACTTTATCTAAAACAACAGTGTACAATACTTTAAATTTATTTGTAGAGAAGAAGTTAGTTTATGTTATAGTTATAGAAGAAAATGAAACAAGATATGATTTATTGACTCATACTCATGGACATTTTAAATGTACttgt                                                                                                           >  1:340838/1‑150 (MQ=255)
                                      cAGAAATACCAACTTTATCTAAAACAACAGTGTACAATACTTTAAATTTATTTGTAGAGAAGAAGTTAGTTTATGTTATAGTTATAGAAGAAAATGAAACAAGATATGATTTATTGACTCATACTCATGGACATTTTAAATGTACttgtt                                                                                                          >  1:2835057/1‑150 (MQ=255)
                                           aTACCAACTTTATCTAAAACAACAGTGTACAATACTTTAAATTTATTTGTAGAGAAGAAGTTAGTTTATGTTATAGTTATAGAAGAAAATGAAACAAGATATGATTTATTGACTCATACTCATGGACATTTTAAATGTACTTGTTGTGGa                                                                                                     <  2:532102/150‑1 (MQ=255)
                                            tACCAACTTTATCTAAAACAACAGTGTACAATACTTTAAATTTATTTGTAGAGAAGAAGTTAGTTTATGTTATAGTTATAGAAGAAAATGAAACAAGATATGATTTATTGACTCATACTCATGGACATTTTAAATGTACTTGTTGTGGAg                                                                                                    >  1:1818466/1‑150 (MQ=255)
                                             aCCAGCTTTATCTAAAACAACAGTGTACAATACTTTAAATTTATTTGTAGAGAAGAAGTTAGTTTATGTTATAGTTATAGAAGAAAATGAAACAAGATATGATTTATTGACTCATACTCATGGACATTTTAAATGTACTTGTTGTGGAGc                                                                                                   <  1:2424273/150‑1 (MQ=255)
                                             aCCAACTTTATCTAAAACAACAGTGTACAATACTTTAAATTTATTTGTAGAGAAGAAGTTAGTTTATGTTATAGTTATAGAAGAAAATGAAACAAGATATGATTTATTGACTCATACTCATGGACATTTTAAATGTACTTGTTGTGGAGc                                                                                                   >  2:616541/1‑150 (MQ=255)
                                              ccAACTTTATCTAAAACAACAGTGTACAATACTTTAAATTTATTTGTAGAGAAGAAGTTAGTTTATGTTATAGTTATAGAAGAAAATGAAACAAGATATGATTTATTGACTCATACTCATGGACATTTTAAATGTACTTGTTGTGGAGCt                                                                                                  >  2:3136942/1‑150 (MQ=255)
                                              ccAACTTTATCTAAAACAACAGTGTACAATACTTTAAATTTATTTGTAGAGAAGAAGTTAGTTTATGTTATAGTTATAGAAGAAAATGAAACAAGATATGATTTATTGACTCATACTCATGGACATTTTAAATGTACTTGTTGTGGAGCt                                                                                                  >  1:3221803/1‑150 (MQ=255)
                                                    ttATCTAAAACAACAGTGTACAATACTTTAAATTTATTTGTAGAGAAGAAGTTAGTTTATGTTATAGTTATAGAAGAAAATGAAACAAGATATGATTTATTGACTCATACTCATGGACATTTTAAATGTACTTGTTGTGGAGCTTTAttt                                                                                            >  2:3394724/1‑150 (MQ=255)
                                                      atcgaaaaCAACAGTGTACAATACTTTAAATTTATTTGTAGAGAAGAAGTTAGTTTATGTTATAGTTATAGAAGAAAATGAAACAAGATATGATTTATTGACTCATACTCATGGACATTTTAAATGTACTTGTTGTGGAGCTTTATTTGa                                                                                          <  2:2557670/146‑1 (MQ=255)
                                                      aTCTAAAACAACAGTGTACAATACTTTAAATTTATTTGTAGAGAAGAAGTTAGTTTATGTTATAGTTATAGAAGAAAATGAAACAAGATATGATTTATTGACTCATACTCATGGACATTTTAAATGTACTTGTTGTGGAGCTTTATTTGa                                                                                          >  1:1001710/1‑150 (MQ=255)
                                                      aTCTAAAACAACAGTGTACAATACTTTAAATTTATTTGTAGAGAAGAAGTTAGTTTATGTTATAGTTATAGAAGAAAATGAAACAAGATATGATTTATTGACTCATACTCATGGACATTTTAAATGTACTTGTTGTGGAGCTTTATTTGa                                                                                          >  1:1570229/1‑150 (MQ=255)
                                                      aTCTAAAACAACAGTGTACAATACTTTAAATTTATTTGTAGAGAAGAAGTTAGTTTATGTTATAGTTATAGAAGAAAATGAAACAAGATATGATTTATTGACTCATACTCATGGACATTTTAAATGTACTTGTTGTGGAGCTTTATTTGa                                                                                          <  1:2470509/150‑1 (MQ=255)
                                                      aTCTAAAACAACAGTGTACAATACTTTAAATTTATTTGTAGAGAAGAAGTTAGTTTATGTTATAGTTATAGAAGAAAATGAAACAAGATATGATTTATTGACTCATACTCATGGACATTTTAAATGTACTTGTTGTGGAGCTTTATTTGa                                                                                          >  1:1617258/1‑150 (MQ=255)
                                                       tCTAAAACAACAGTGTACAATACTTTAAATTTATTTGTAGAGAAGAAGTTAGTTTATGTTATAGTTATAGAAGAAAATGAAACAAGATATGATTTATTGACTCATACTCATGGACATTTTAAATGTACTTGTTGTGGAGCTTTATTTGAt                                                                                         >  1:2383041/1‑150 (MQ=255)
                                                        tgaaaaCAACAGTGTACAATACTTTAAATTTATTTGTAGAGAAGAAGTTAGTTTATGTTATAGTTATAGAAAAAAATGAAACAAGATATAATTTATTGACTCATACTCATGGACATTTTAAATGTACTTGTTGTGGAGCTTTATTTGATg                                                                                        <  2:370318/148‑1 (MQ=255)
                                                        cTAAAACAACAGTGTACAATACTTTAAATTTATTTGTAGAGAAGAAGTTAGTTTATGTTATAGTTATAGAAGAAAATGAAACAAGATATGATTTATTGACTCATACTCATGGACATTTTAAATGTACTTGTTGTGGAGCTTTATTTGATg                                                                                        <  2:3192800/150‑1 (MQ=255)
                                                         tAAAACAACAGTGTACAATACTTTAAATTTATTTGTAGAGAAGAAGTTAGTTTATGTTATAGTTATAGAAGAAAATGAAACAAGATATGATTTATTGACTCATACTCATGGACATTTTAAATGTACTTGTTGTGGAGCTTTATTTGATGt                                                                                       >  2:298338/1‑150 (MQ=255)
                                                          aaaaCAACAGTGTACAATACTTTAAATTTATTTGTAGAGAAGAAGTTAGTTTATGTTATAGTTATAGAAGAAAATGAAACAAGATATGATTTATTGACTCATACTCATGGACATTTTAAATGTACTTGTTGTGGAGCTTTATTTGATGTa                                                                                      >  1:2699414/1‑150 (MQ=255)
                                                            aacaacAGTGTACAATACTTTAAATTTATTTGTAGAGAAGAAGTTAGTTTATGTTATAGTTATAGAAGAAAATGAAACAAGATATGATTTATTGACTCATACTCATGGACATTTTAAATGTACTTGTTGTGGAGCTTTATTTGATGTAGa                                                                                    <  2:1256003/150‑1 (MQ=255)
                                                            aacaacAGTGTACAATACTTTAAATTTATTTGTAGAGAAGAAGTTAGTTTATGTTATAGTTATAGAAGAAAATGAAACAAGATATGATTTATTGACTCATACTCATGGACATTTTAAATGTACTTGTTGTGGAGCTTTATTTGATGTAGa                                                                                    <  2:1673277/150‑1 (MQ=255)
                                                                  aGTGTACAATACTTTAAATTTATTTGTAGAGAAGAAGTTAGTTTATGTTATAGTTATAGAAGAAAATGAAACAAGATATGATTTATTGACTCATACTCATGGACATTTTAAATGTACTTGTTGTGGAGCTTTATTTGATGTAGAATTaaa                                                                              <  1:1724288/150‑1 (MQ=255)
                                                                      tACAATACTTTAAATTTATTTGTAGAGAAGAAGTTAGTTTATGTTATAGTTATAGAAGAAAATGAAACAAGATATGATTTATTGACTCATACTCATGGACATTTTAAATGTACTTGTTGTGGAGCTTTATTTGATGTAGAATTAAATAtt                                                                          >  2:234300/1‑150 (MQ=255)
                                                                      tACAATACTTTAAATTTATTTGTAGAGAAGAAGTTAGTCTATGTTATAGTTATAGAAGAAAATGAAACAAGATATGATTTATTGACTCATACTCATGGACATTTTAAATGTACTTGTTGTGGAGCTTTATTTGATGTAGAATTAAATAtt                                                                          >  2:664172/1‑150 (MQ=255)
                                                                       ccAATACTTTAAATTTATTTATAGAGAAGAAGTTAGTTTATGTTATAGTTATAGAAGAAAATGAAACAAGATATGATTTATTGACTCATACTCATGGACATTTTAAATGTACTTGTTGTGGAGCTTTATTTGATGTAGAATTAAATATTg                                                                         <  2:3048695/149‑1 (MQ=255)
                                                                        cAATACTTTAAATTTATTTGTAGAGAAGAAGTTAGTTTANGTTATAGTTATAGAAGAAAATGAAACAAGATATGATTTATTGACTCATACTCATGGACATTTTAAATGTACTTGTTGTGGAGCTTTATTTGATGTAGAATTAAATATTGa                                                                        >  1:2632602/1‑150 (MQ=255)
                                                                            aCTTTAAATTTATTTGTAGAGAAGAAGTTAGTTTATGTTATAGTTATAGAAGAAAATGAAACAAGATATGATTTATTGACTCATACTCATGGACATTTTAAATGTACTTGTTGTGGAGCTTTATTTGATGTAGAATTAAATATTGATtat                                                                    >  2:1752056/1‑150 (MQ=255)
                                                                             cTTTAAATTTATTTGTAGAGAAGAAGTTAGTTTATGTTATAGTTATAGAAGAAAATGAAACAAGATATGATTTATTGACTCATACTCATGGACATTTTAAATGTACTTGTTGTGGAGCTTTATTTGATGTAGAATTAAATATTGATtata                                                                   <  1:1980192/150‑1 (MQ=255)
                                                                              tttAAATTTATTTGTAGAGAAGAAGTTAGTTTATGTTATAGTTATAGAAGAAAATGAAACAAGATATGATTTATTGACTCATACTCATGGACATTTTAAATGTACTTGTTGTGGAGCTTTATTTGATGTAGAATTAAATATTGATTATAg                                                                  <  1:1461504/150‑1 (MQ=255)
                                                                               ttAAATTTATTTGTAGAGAAGAAGTTAGTTTATGTTATAGTTATAGAAGAAAATGAAACAAGATATGATTTATTGACTCATACTCATGGACATTTTAAATGTACTTGTTGTGGAGCTTTATTTGATGTAGAATTAAATATTGATTATAGc                                                                 <  1:2756321/150‑1 (MQ=255)
                                                                               ttAAATTTAGTTGTAGAGAAGAAGTTAGTTTATGTTATAGTTATAGAAGAAAATGAAACAAGATATGATTTATTGACTCATACTCATGGACATTTTAAATGTACTTGTTGTGGAGCTTTATTTGATGTAGAATTAAATATTGATTATAGc                                                                 <  1:3270148/150‑1 (MQ=255)
                                                                                 aaaTTTATTTGTAGAGAAGAAGTTAGTTTATGTTATAGTTATAGAAGAAAATGAAACAAGATATGATTTATTGACTCATACTCATGGACATTTTAAATGTACTTGTTGTGGAGCTTTATTTGATGTAGAATTAAATATTGATTATAGCaa                                                               >  1:1509374/1‑150 (MQ=255)
                                                                                  aaTTTATTTGTAGAGAAGAAGTTAGTTTATGTTATAGTTATAGAAGAAAATGAAACAAGATATGATTTATTGACTCATACTCATGGACATTTTAAATGTACTTGTTGTGGAGCTTTATTTGATGTAGAATTAAATATTGATTATAGCaaa                                                              >  1:784262/1‑150 (MQ=255)
                                                                                    tttatttGTAGAGAAGAAGTTAGTTTATGTTATAGTTATAGAAGAAAATGAAACAAGATATGATTTATTGACTCATACTCATGGACATTTTAAATGTACTTGTTGTGGAGCTTTATTTGATGTAGAATTAAATATTGATTATAGCAAAAg                                                            <  1:513210/150‑1 (MQ=255)
                                                                                    tttatttGTAGAGAAGAAGTTAGTTTATGTTATAGTTATAGAAGAAAATGAAACAAGATATGATTTATTGACTCATACTCATGGACATTTTAAATGTACTTGTTGTGGAGCTTTATTTGATGTAGAATTAAATATTGATTATAGCAAAAg                                                            <  1:2280045/150‑1 (MQ=255)
                                                                                      tatttGTAGAGAAGAAGTTAGTTTATGTTATAGTTATAGAAGAAAATGAAACAAGATATGATTTATTGACTCATACTCATGGACATTTTAAATGTACTTGTTGTGGAGCTTTATTTGATGTAGAATTAAATATTGATTATAGCAAAAGTc                                                          >  2:591486/1‑150 (MQ=255)
                                                                                        tttGTAGAGAAGAAGTTAGTTTATGTTATAGTTATAGAAGAAAATGAACCAAGATATGATTTATTTCCTCATACTCATGGACATTTTAAACGTACTTGTTGTGGAGCTTTATTTGATGTAGAATTAAATATTGATTATAGCAAAAGTCaa                                                        >  1:656404/1‑150 (MQ=255)
                                                                                         ttGTAGAGAAGAAGTTAGTTTATGTTATGGTTATAGAAGAAAATGAAACAAGATATGATTTATTGACTCATACTCATGGACATTTTAAATGTACTTGTTGTGGAGCTTTATTTGATGTAGAATTAAATATTGATTATAGCAAAAGTCAAg                                                       <  2:75272/150‑1 (MQ=255)
                                                                                         ttGTAGAGAAGAAGTTAGTTTATGTTATAGTTATAGAAGAAAATGAAACAAGATATGATTTATTGACTCATACTCATGGACATTTTAAATGTACTTGTTGTGGAGCTTTATTTGATGTAGAATTAAATATTGATTATAGCAAAAGTCAAg                                                       <  2:1989977/150‑1 (MQ=255)
                                                                                           gTAGAGAAGAAGTTAGTTTATGTTATAGTTATAGAAGAAAATGAAACAAGATATGATTTATTGACTCATACTCATGGACATTTTAAATGTACTTGTTGTGGAGCTTTATTTGATGTAGAATTAAATATTGATTATAGCAAAAGTCAAGaa                                                     <  2:1106715/150‑1 (MQ=255)
                                                                                           gTAGAGAAGAAGTTAGTTTATGTTATAGTTATAGAAGAAAATGAAACAAGATATGATTTATTGACTCATACTCATGGACATTTTAAATGTACTTGTTGTGGAGCTTTATTTGATGTAGAATTAAATATTGATTATAGCAAAAGTCAAGaa                                                     >  2:137656/1‑150 (MQ=255)
                                                                                            tAGAGAAGAAGTTAGTTTATGTTATAGTTATAGAAGAAAATGAAACAAGATATGATTTATTGACTCATACTCATGGACATTTTAAATGTACTTGTTGTGGAGCTTTATTTGATGTAGAATTAAATATTGATTATAGCAAAAGTCAAGAat                                                    <  2:443928/150‑1 (MQ=255)
                                                                                               agaagaAGTTAGTTTATGTTATAGTTATAGAAGAAAATGAAACAAGATATGATTTATTGACTCATACTCATGGACATTTTAAATGTACTTGTTGTGGAGCTTTATTTGATGTAGAATTAAATATTGATTATAGCAAAAGTCAAGAattat                                                 <  2:1702117/150‑1 (MQ=255)
                                                                                               agaagaAGTTAGTTTATGTTATAGTTATAGAAGAAAATGAAACAAGATATGATTTATTGACTCATACTCATGGACATTTTAAATGTACTTGTTGTGGAGCTTTATTTGATGTAGAATTAAATATTGATTATAGCAAAAGTCAAGAattat                                                 <  1:2012089/150‑1 (MQ=255)
                                                                                               agaagaAGTTAGTTTATGTTATAGTTATAGAAGAAAATGAAACAAGATATGATTTATTGACTCATACTCATGGACATTTTAAATGTACTTGTTGTGGAGCTTTATTTGATGTAGAATTAAATATTGATTATAGCAAAAGTCAAGAattat                                                 >  2:2222174/1‑150 (MQ=255)
                                                                                                 aagaagTTAGTTTATGTTATAGTTATAGAAGAAAATGAAACAAGATATGATTTATTGACTCATACTCATGGACATTTTAAATGTACTTGTTGTGGAGCTTTATTTGATGTAGAATTAAATATTGATTATAGCAAAAGTCAAGAAttatta                                               >  1:1248007/1‑150 (MQ=255)
                                                                                                  agaagTTAGTTTATGTTATAGTTATAGAAGAAAATGAAACAAGATATGATTTATTGACTCATACTCATGGACATTTTAAATGTACTTGTTGTGGAGCTTTATTTGATGTAGAATTAAATATTGATTATAGCAAAAGTCAAGAATTATTAg                                              >  1:1803870/1‑150 (MQ=255)
                                                                                                   gaagTTAGTTTATGTTATAGTTATAGAAGAAAATGAAACAAGATATGATTTATTGACTCATACTCATGGACATTTTAAATGTACTTGTTGTGGAGCTTTATTTGATGTAGAATTAAATATTGATTATAGCAAAAGTCAAGAATTATTAgg                                             <  1:3159743/150‑1 (MQ=255)
                                                                                                   gaagTTAGTTTATGTTATAGTTATAGAAGAAAATGAAACAAGATATGATTTATTGACTCATACTCATGGACATTTTAAATGTACTTGTTGTGGAGCTTTATTTGATGTAGAATTAAATATTGATTATAGCAAAAGTCAAGAATTATTAgg                                             <  2:2638088/150‑1 (MQ=255)
                                                                                                       ttagttTATGTTATAGTTATAGAAGAAAATGAAACAAGATATGATTTATTGACTCATACTCATGGACATTTTAAATGTACTTGTTGTGGAGCTTTATTTGATGTAGAATTAAATATTGATTATAGCAAAAGTCAAGAATTATTAGGCtgt                                         <  1:2233550/150‑1 (MQ=255)
                                                                                                         agttTATGTTATAGCTATAGAAGAAAATGAAACAAGATATGATTTATTGACTCATACTCATGGACATTTTAAATGTACTTGTTGTGGAGCTTTATTTGATGTAGAATTAAATATTGATTATAGCAAAAGTCAAGAATTATTAGGCTGTGa                                       <  1:1647991/150‑1 (MQ=255)
                                                                                                          gttTATGTTATAGTTATAGAAGAAAATGAAACAAGATATGATTTATTGACTCATACTCATGGACATTTTAAATGTACTTGTTGTGGAGCTTTATTTGATGTAGAATTAAATATTGATTATAGCAAAAGTCAAGAATTATTAGGCTGTGat                                      >  2:810880/1‑150 (MQ=255)
                                                                                                              aTGTTATAGTTATAGAAGAAAATGAAACAAGATATGATTTATTGACTCATACTCATGGACATTTTAAATGTACTTGTTGTGGAGCTTTATTTGATGTAGAATTAAATATTGATTATAGCAAAAGTCAAGAATTATTAGGCTGTGATATTg                                  <  1:107205/150‑1 (MQ=255)
                                                                                                                gttatagttataGAAGAAAATGAAACAAGATATGATTTATTGACTCATACTCATGGACATTTTAAATGTACTTGTTGTGGAGCTTTATTTGATGTAGAATTAAATATTGATTATAGCAAAAGTCAAGAATTATTAGGCTGTGATATTGaa                                >  2:1512648/1‑150 (MQ=255)
                                                                                                                gttatagttataGAAGAAAATGAAACAAGATATGATTTATTGACTCATACTCATGGACATTTTAAATGTACTTGTTGTGGAGCTTTATTTGATGTAGAATTAAATATTGATTATAGCAAAAGTCAAGAATTATTAGGCTGTGATATTGaa                                >  2:1713581/1‑150 (MQ=255)
                                                                                                                 ttatagttatagAAGAAAATGAAACAAGATATGATTTATTGACTCATACTCATGGACATTTTAAATGTACTTGTTGTGGAGCTTTATTTGATGTAGAATTAAATATTGATTATAGCAAAAGTCAAGAATTATTAGGCTGTGATATTGAag                               >  2:2287457/1‑150 (MQ=255)
                                                                                                                 ttatagttatagAAGAAAATGAAACAAGATATGATTTATTGACTCATACTCATGGACATTTTAAATGTACTTGTTGTGGAGCTTTATTTGATGTAGAATTAAATATTGATTATAGCAAAAGTCAAGAATTATTAGGCTGTGATATTGAag                               <  2:3084903/150‑1 (MQ=255)
                                                                                                                  tatagttatagAAGAAAATGAAACAAGATATGATTTATTGACTCATACTCATGGACATTTTAAATGTACTTGTTGTGGAGCTTTATTTGATGTAGAATTAAATATTGATTATAGCAAAAGTCAAGAATTATTAGGCTGTGATATTGAaga                              >  1:441977/1‑150 (MQ=255)
                                                                                                                  tatagttatagAAGAAAATGAAACAAGATATGATTTATTGACTCATACTCATGGACATTTTAAATGTACTTGTTGTGGAGCTTTATTTGATGTAGAATTAAATATTGATTATAGCAAAAGTCAAGAATTATTAGGCTGTGATATTGAaga                              <  2:2933960/150‑1 (MQ=255)
                                                                                                                    tagttatagAAGAAAATGAAACAAGATATGATTTATTGACTCATACTCATGGACATTTTAAATGTACTTGTTGTGGAGCTTTATTTGATGTAGAATTAAATATTGATTATAGCAAAAGTCAAGAATTATTAGGCTGTGATATTGAAgaga                            <  2:2040092/150‑1 (MQ=255)
                                                                                                                      gttatagAAGAGAATGAAACAAGATATGATTTATTGACTCATACTCATGGACATTTTAAATGTACTTGTTGTGGAGCTTTATTTGATGTAGAATTAAATATTGATTATAGCAAAAGTCAAGAATTATTAGGCTGTGATATTGAAGAGaaa                          <  2:898567/150‑1 (MQ=255)
                                                                                                                      gttatagAAGAAAATGAAACAAGATATGATTTATTGACTCATACTCATGGACATTTTAAATGTACTTGTTGTGGAGCTTTATTTGATGTAGAATTAAATATTGATTATAGCAAAAGTCAAGAATTATTAGGCTGTGATATTGAAGAGaaa                          >  1:258173/1‑150 (MQ=255)
                                                                                                                      gttatagAAGAAAATGAAACAAGATATGATTTATTGACTCATACTCATGGACATTTTAAATGTACTTGTTGTGGAGCTTTATTTGATGTAGAATTAAATATTGATTATAGCAAAAGTCAAGAATTATTAGGCTGTGATATTGAAGAGaaa                          >  1:3492807/1‑150 (MQ=255)
                                                                                                                       ttatagAAGAAAATGAAACAAGATATGATTTATTGACTCATACTCATGGACATTTTAAATGTACTTGTTGTGGAGCTTTATTTGATGTAGAATTAAATATTGATTATAGCAAAAGTCAAGAATTATTAGGCTGTGATATTGAAGAGAAAc                         <  1:1210968/150‑1 (MQ=255)
                                                                                                                       ttatagAAGAAAATGAAACAAGATATGATTTATTGACTCATACTCATGGACATTTTAAATGTACTTGTTGTGGAGCTTTATTTGATGTAGAATTAAATATTGATTATAGCAAAAGTCAAGAATTATTAGGCTGTGATATTGAAGAGAAAc                         <  1:979384/150‑1 (MQ=255)
                                                                                                                       ttatagAAGAAAATGAAACAAGATATGACTTATTGACTCATACTCATGGACATTTTAAATGTACTTGTTGTGGAGCTTTATTTGATGTAGAATTAAATATTGATTATAGCAAAAGTCAAGAATTATTAGGCTGTGATATTGAAGAGAAAc                         <  2:1606697/150‑1 (MQ=255)
                                                                                                                           agaagaAAATGAAACAAGATATGATTTATTGACTCATACTCATGGACATTTTAAATGTACTTGTTTTGGAGCTTTATTTGATGTAGAATTAAATATTGATTATAGCAAAAGTCAAGAATTATTAGGCTGTGATATTGAAGAGAAACatat                     <  2:1152069/150‑1 (MQ=255)
                                                                                                                           agaagaAAATGAAACAAGATATGATTTATTGACTCATACTCATGGACATTTTAAATGTACTTGTTGTGGAGCTTTATTTGATGTAGAATTAAATATTGATTATAGCAAAAGTCAAGAATTATTAGGCTGTGATATTGAAGAGAAACatat                     >  2:2419864/1‑150 (MQ=255)
                                                                                                                            gaagaaAATGAAACAAGATATGATTTATTGACTCATACTCATGGACATTTTAAATGTACTTGTTGTGGAGCTTTATTTGATGTAGAATTAAATATTGATTATAGCAAAAGTCAAGAATTATTAGGCTGTGATATTGAAGAGAAACatata                    >  1:190170/1‑150 (MQ=255)
                                                                                                                            gaagaaAATGAAACAAGATATGATTTATTGACTCATACTCATGGACATTTTAAATGTACTTGTTGTGGAGCTTTATTTGATGTAGAATTAAATATTGATTATAGCAAAAGTCAAGAATTATTAGGCTGTGATATTGAAGAGAAACatata                    <  2:3312342/150‑1 (MQ=255)
                                                                                                                             aagaaAATGAAACAAGATATGATTTATTGACTCATACTCATGGACATTTTAAATGTACTTGTTGTGGAGCTTTATTTGATGTAGAATTAAATATTGATTATAGCAAAAGTCAAGAATTATTAGGCTGTGATATTGAAGAGAAACatatat                   <  1:2062390/150‑1 (MQ=255)
                                                                                                                              agaaAATGAAACAAGATATGATTTATTGACTCATACTCATGGACATTTTAAATGTACTTGTTGTGGAGCTTTATTTGATGTAGAATTAAATATTGATTATAGCAAAAGTCAAGAATTATTAGGCTGTGATATTGAAGAGAAACatatata                  >  2:3470871/1‑150 (MQ=255)
                                                                                                                               gaaAATGAAACAAGATATGATTTATTGACTCATACTCATGGACATTTTAAATGTACTTGTTGTGGAGCTTTATTTGATGTAGAATTAAATATTGATTATAGCAAAAGTCAAGAATTATTAGGCTGTGATATTGAAGAGAAACatatatat                 >  1:3454527/1‑150 (MQ=255)
                                                                                                                                 aaaTGAAACAAGATATGATTTATTGACTCATACTCATGGACATTTTAAATGTACTTGTTGTGGAGCTTTATTTGATGTAGAATTAAATATTGATTATAGCAAAAGTCAAGAATTATTAGGCTGTGATATTGAAGAGAAACATATATAttt               >  1:1119630/1‑150 (MQ=255)
                                                                                                                                 aaaTGAAACAAGATATGATTTATTGACTCATACTCATGGACATTTTAAATGTACTTGTTGTGGAGCTTTATTTGATGTAGAATTAAATATTGATTATAGCAAAAGTCAAGAATTATTAGGCTGTGATATTGAAGAGAAACATATATAttt               >  1:592292/1‑150 (MQ=255)
                                                                                                                                 aaaTGAAACAAGATATGATTTATTGACTCATACTCATGGACATTTTAAATGTACTTGTTGTGGAGCTTTATTTGATGTAGAATTAAATATTGATTATAGCAAAAGTCAAGAATTATTAGGCTGTGATATTGAAGAGAAACATATATAttt               >  2:1553624/1‑150 (MQ=255)
                                                                                                                                  aaTGAAACAAGATATGATTTATTGACTCATACTCATGGACATTTTAAATGTACTTGTTGTGGAGCTTTATTTGATGTAGAATTAAATATTGATTATAGCAAAAGTCAAGAATTATTAGGCTGTGATATTGAAGAGAAACATATATAtttt              <  1:3093620/150‑1 (MQ=255)
                                                                                                                                  aaTGAAACAAGATATGATTTATTGACTCATACTCATGGACATTTTAAATGTACTTGTTGTGGAGCTTTATTTGATGTAGAATTAAATATTGATTATAGCAAAAGTCAAGAATTATTAGGCTGTGATATTGAAGAGAAACATATATAtttt              >  1:3021839/1‑150 (MQ=255)
                                                                                                                                      aaaCAAGATATGATTTATTGACTCATACTCATGGACATTTTAAATGTACTTGTTGTGGAGCTTTATTTGATGTAGAATTAAATATTGATTATAGCAAAAGTCAAGAATTATTAGGCTGTGATATTGAAGAGAAACATATGTATTTTAAAg          <  2:1945666/150‑1 (MQ=255)
                                                                                                                                         cAAGATATGATTTATTGACTCATACTCATGGACATTTTAAATGTACTTGTTGTGGAGCTTTATTTGATGTAGAATTAAATATTGATTATAGCAAAAGTCAAGAATTATTAGGCTGTGATATTGAAGAGAAACATATATATTTTAAAGGCa       <  2:1214464/150‑1 (MQ=255)
                                                                                                                                           aGATATGATTTATTGGCTCATACTCATGGACATTTTAAATGTACTTGTTGTGGAGCTTTATTTGATGTAGAATTAAATATTGATTATAGCAAAAGTCAAGAATTATTAGGCTGTGATATTGAAGAGAAACATATATATTTTAAAGGCata     <  2:1799897/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
TCATCCAACAGTAGATACAATATATAAAGCACTTTGTACAGAAATACCAACTTTATCTAAAACAACAGTGTACAATACTTTAAATTTATTTGTAGAGAAGAAGTTAGTTTATGTTATAGTTATAGAAGAAAATGAAACAAGATATGGTTTATTGACTCATACTCATGGACATTTTAAATGTACTTGTTGTGGAGCTTTATTTGATGTAGAATTAAATATTGATTATAGCAAAAGTCAAGAATTATTAGGCTGTGATATTGAAGAGAAACATATATATTTTAAAGGCATATGT  >  CP028101/515886‑516177

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑