Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0281011,847,1310CT24.4% 682.6 / 47.0 402E718K (GAA→AAA) C7Y58_08910CRISPR‑associated helicase/endonuclease Cas3
Reads supporting (aligned to +/- strand):  ref base C (105/171);  new base T (89/0);  total (231/171)
Fisher's exact test for biased strand distribution p-value = 1.77e-30
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 424 total aligned reads displayed.

TTTACCTTTTAAAATTTCACTTATATACTTACTTAACTGATAGTATTGGATTGAAAGAGTCTTCTTTAGAAGTTCAGTTTTAGCATTCTGTCTTTCTTCTAAACCTAAATTTTTATCTTCTATCTTTTTTATAAGTTCCTTTATATTTTCTT‑CATTTTCCTCATAAATATCATATGGAATTACAGTAACACTTTGGATATCCCTAAGCTTTAAAGCATCTTGTGAATTTTCATCACTATTTAAAATTCTTTGGTATTTTTCATAAGCTTTTTCAAATTCATCAACAAAATTACTTTCTT  >  CP028101/1846982‑1847280
                                                                                                                                                     |                                                                                                                                                      
 ttACCTTTTAAAATTTCACTTATATACTTACTTAACTGATAGTATTGGATTGAAAGAGTCTTCTTTAGAAGTTTAGTTTTAGCATTCTGTCCTTCTTCTAAACCTAAATTTTTATCTTCTATCTTTTTTATAAGTTCCTTTATATTttct                                                                                                                                                       <  1:3271509/150‑1 (MQ=255)
    ccTTTTAAAATTTCACTTATATACTTACTTAACTGATAGTATTGGATTGAAAGAGTCTTCTTTAGAAGTTCAGTTTTAGCATTCTGTCTTTCTTCTAAACCTAAATTTTTATCTTCTATCTTTTTTATAAGTTCCTTTATATTTTCTT‑Ca                                                                                                                                                   <  2:1886673/150‑1 (MQ=255)
     cTTTTAAAATTTCACTTATATACTTACTTAACTGATAGTATTGGATTGAAAGAGTCTTCTTTAGAAGTTCAGTTTTAGCATTCTGTCTTTCTTCTAAACCTAAATTTTTATCTTCTATCTTTTTTATAAGTTCCTTTATATTTTTTT‑CAt                                                                                                                                                  >  1:2302493/1‑150 (MQ=255)
     cTTTTAAAATTTCACTTATATACTTACTTAACTGATAGTATTGGATTGAAAGAGTCTTCTTTAGAAGTTCAGTTTTAGCATTCTGTCTTTCTTCTAAACCTAAATTTTTATCTTCTATCTTTTTTATAAGTTCCTTTATATTTTTTT‑CAt                                                                                                                                                  >  2:1160270/1‑150 (MQ=255)
     cTTTTAAAATTTCACTTATATACTTACTTAACTGATAGTATTGGATTGAAAGAGTCTTCTTTAGAAGTTCAGTTTTAGCATTCTGTCTTTCTTCTAAACCTAAATTTTTATCTTCTATCTTTTTTATAAGTTCCTTTATATTTTGTT‑CAt                                                                                                                                                  >  1:828519/1‑150 (MQ=255)
      ttttAAAATTTCACTTATATACTTACTTAACTGATAGTATTGGATTGAAAGAGTCTTCTTTAGAAGTTCAGTTTTAGCATTCTGTCTTTCTTCTAAACCTAAATTTTTATCTTCTATCTTTTTTATAAGTTCCTTTATATTTTTTT‑CAtt                                                                                                                                                 >  2:2954180/1‑150 (MQ=255)
          aaaaTTTCACTTATATACTTACTTAACTGATAGTATTGGATTGAAAGAGTCTTCTTTAGAAGTTCAGTTTTAGCATTCTGTCTTTCTTCTAAACCTAAATTTTTATCTTCTATCTTTTTTATAAGTTCCTTTATATTTTCTT‑CATTTTcc                                                                                                                                             <  1:3456237/150‑1 (MQ=255)
            aaTTTCACTTATATACTTACTTAACTGATAGTATTGGATTGAAAGAGTCTTCTTTAGAAGTTCAGTTTTAGCATTCTGTCTTTCTTCTAAACCTAAATTTTTATCTTCTATCTTTTTTATAAGTTCCTTTATATTTTTTT‑CATTTTCCTc                                                                                                                                           >  1:3228901/1‑150 (MQ=255)
                 cACTTCTATACTTACTTAACTGATAGTATTGGATTGAAAGAGTCTTCTTTCGAAGTTCCGTTTTAGCATTCTGTCTTTCTTTTAAACCTAAATTTTTCTCTTCTATCTTTTTTCTAAGTTCCTTTGTATTTTTTT‑TATTTTCCTCATaaa                                                                                                                                      >  2:2893320/1‑150 (MQ=255)
                 cACTTATATACTTACTTAACTGATAGTATTGGATTGAAAGAGTCTTCTTTAGAAGTTCAGTTTTAGCATTCTGTCTTTCTTCTAAACCTAAATTTTTATCTTCTATCTTTTTTATAAGTTCCTTTATATTTTCTT‑CATTTTCCTCATaaa                                                                                                                                      <  2:2417979/150‑1 (MQ=255)
                  aCTTATATACTTACTTAACTGATAGTATTGGATTGAAAGAGTCTTCTTTAGAAGTTCAGTTTTAGCATTCTGTCTTTCTTCTAAACCTAAATTTTTATCTTCTATCTTTTTTATAAGTTCCTTTATATTTTCTT‑CATTTTCCTCATAAat                                                                                                                                     <  1:2265743/150‑1 (MQ=255)
                    ttATATACTTACTTAACTGATAGTATTGGATTGAAAGAGTCTTCTTTAGAAGTTCAGTTTTAGCATTCTGTCTTTCTTCTAAACCTAAATTTTTATCTTCTATCTTTTTTATAAGTTCCTT‑ATATTTTCTT‑CATTTTCCTCATAAATATc                                                                                                                                  <  1:2063762/150‑1 (MQ=255)
                     tatataCTTACTTAACTGATAGTATTGGATTGAAAGAGTCTTCTTTAGAAGTTCAGTTTTAGCATTCTGTCTTTCTTCTAAACCTAAATTTTTATCTTCTATCTTTTTTATAAGTTCCTTTATATTTTTTT‑CATTTTCCTCATAAATATc                                                                                                                                  >  2:1647389/1‑150 (MQ=255)
                       tataCTTACTTAACTGATAGTATTGGATTGAAAGAGTCTTCTTTAGAAGTTCAGTTTTAGCATTCTGTCTTTCTTCTAAACCTAAATTTTTATCTTCTATCTTTTTTATAAGTTCCTTTATATTTTTTT‑CATTTTCCTCATAAATATCat                                                                                                                                >  2:1515856/1‑150 (MQ=255)
                         tacttactTAACTGATAGTATTGGATTGAAAGAGTCTTCTTTAGAAGTTCAGTTTTAGCATTCTGTCTTTCTTCTAAACCTAAATTTTTATCTTCTATCTTTTTTATAAGTTCCTTTATATTTTCTT‑CATTTTCCTCATAAATATCatat                                                                                                                              >  2:593560/1‑150 (MQ=255)
                           cttacttaACTGATAGTATTGGATTGAAAGAGTCTTCTTTAGAAGTTCAGTTTTAGCATTCTGTCTTTCTTCTAAACCTAAATTTTTCTTTTCTACTTTTTTTCTAAGTTCCTTTCTCTTTTGTT‑CCTTTTCTTCACAAACACCatatag                                                                                                                            >  2:2193314/1‑148 (MQ=255)
                            ttacttaACTGATAGTATTGGATTGAAAGATTCTTCTTTAGAAGTTCAGTTTTAGCATTCTGTCTTTCTTCTAAACCTAAATTTTTATCTTCTATCTTTGTTATAAGTTCCTTTATATTTTCTT‑CATTTTCCTCATAAATATCATATGGa                                                                                                                           <  2:3012792/150‑1 (MQ=255)
                             tacttaACTGATAGTATTGGATTGAAAGAGTCTTCTTTAGAAGTTCAGTTTTAGCATTCTGTCTTTCTTCTAAACCTAAATTTTTATCTTCTATCTTTTTTATAAGTTTCTTTGTATTTTCTT‑CATTTTCCTCATAAATATCATATGGaa                                                                                                                          >  2:2457026/1‑150 (MQ=255)
                              acttaACTGATAGTATTGGATTGAAAGAGTCTTCTTTAGAAGTTCAGTTTTAGCATTCTGTCTTTCTTCTAAACCTAAATTTTTATCTTCTATCTTTTTTATAAGTTCCTTTATATTTTCTT‑CATTTTCCTCATAAATATCATATGGAAt                                                                                                                         <  2:896098/150‑1 (MQ=255)
                               cttaACTGATAGTATTGGATTGAAAGAGTCTTCTTTAGAAGTTCAGTTTTAGCATTCTGTCTTTCTTCTAAACCTAAATTTTTATCTTCTATCTTTTTTATCAGTTCCTTTATATTTTCTT‑CATTTTCCTCATACCTATCATATGGAAtt                                                                                                                        >  2:2166100/1‑150 (MQ=255)
                               cttaACTGATAGTATTGGATTGAAAGAGTCTTCTTTAGAAGTTCAGTTTTAGCATTCTGTCTTTCTTCTAAACCTAAATTTTTATCTTCTATCTTTTTTATAAGTTCCTTTATATTTTCTT‑CATTTTCCTCATAAATATCATATGGAAtt                                                                                                                        <  1:1012628/150‑1 (MQ=255)
                                 taACTGATAGTATTGGATTGAAAGAGTCTTCTTTAGAAGTTCAGTTTTAGCATTCTGTCTTTCTTCTAAACCTAAATTTTTATCTTCTATCTTTTTTATAAGTTCCTTTATATTTTTTT‑CATTTTCCTCATAAATATCATATGGAATTAc                                                                                                                      >  1:1762271/1‑150 (MQ=255)
                                 taACTGATAGTATTGGATTGAAAGAGTCTTCTTTAGAAGTTCAGTTTTAGCATTCTGTCTTTCTTCTAAACCTAAATTTTTATCTTCTATCTTTTTTATAAGTTCCTTTATATTTTCTT‑CCTTTTCCTCATAAATATCATATGGAATTAc                                                                                                                      >  2:3199163/1‑150 (MQ=255)
                                 taACTGATAGTATTGGATTGAAAGAGTCTTCTTTAGAAGTTCAGTTTTAGCATTCTGTCTTTCTTCTAAACCTAAATTTTTATCTTCTATCTTTTTTATAAGTTCCTTTATATTTTCTT‑CATTTTCCTCATAAATATCATATGGAATTAc                                                                                                                      <  2:1464154/150‑1 (MQ=255)
                                 taACTGATAGTATTGGATTGAAAGAGTCTTCTTTAGAAGTTCAGTTTTAGCATTCTGTCTTTCTTCTAAACCTAAATTTTTATCTTCTATCTTTTTTATAAGTTCCTTTATATTTTCTT‑CATTTTCCTCATAAATATCATATGGAATTAc                                                                                                                      <  1:591086/150‑1 (MQ=255)
                                 taACTGATAGTATTGGATTGAAAGAGTCTTCTTTAGAAGTTCAGTTTTAGCATTCTGTCTTTCTTCTAAACCTAAATTTTTATCTTCTATCTTTTTTATAAGTTCCTTTATATTTTCTT‑CATTTTCCTCATAAATATCATATGGAATTAc                                                                                                                      <  1:3488374/150‑1 (MQ=255)
                                 taACTGATAGTATTGGATTGAAAGAGTCTTCTTTAGAAGTTCAGTTTTAGCATTCTGTCTTTCTTCTAAACCTAAATTTTTATCTTCTATCTTTTTTATAAGTTCCTTTATATTTTCTT‑CATTTTCCTCATAAATATCATATGGAATTAc                                                                                                                      >  2:3446867/1‑150 (MQ=255)
                                 taACTGATAGTATTGGATTGAAAGAGTCTTCTTTAGAAGTTCAGTTTTAGCATTCTGTCTTTCTTCTAAACCTAAATTTTTATCTTCTATCTTTTTTATAAGTTCCTTTATATTTTCTT‑CATTTTCCTCATAAATATCATATGGAATTAc                                                                                                                      <  2:402968/150‑1 (MQ=255)
                                   aCTGATAGTATTGGATTGAAAGAGTCTTCTTTAGAAGTTCAGTTTTAGCATTCTGTCTTTCTTCTAAACCTAAATTTTTATCTTCTATCTTTTTTATAAGTTCCTTTATATTTTCTT‑CATTTTCCTCATAAATATCATATGGAATTACAg                                                                                                                    <  1:1413432/150‑1 (MQ=255)
                                    cTGATAGTATTGGATTGAAAGAGTCTTCTTTAGAAGTTCAGTTTTAGCATTCTGTCTTTCTTCTAAACCTAAATTTTTATCTTCTATCTTTTTTATAAGTTCCTTTATATTTTTTT‑CATTTTCCTCATAAATATCATATGGAATTACAGt                                                                                                                   >  1:2479516/1‑150 (MQ=255)
                                     tGATAGTATTGGATTGAAAGAGTCTTCTTTAGAAGTTCAGTTTTAGCATTCTGTCTTTCTTCTAAACCTAAATTTTTATCTTCTATCTTTTTTATAAGTTCCTTTATATTTTTTT‑CATTTTCCTCATAAATATCATATGGAATTACAGTa                                                                                                                  >  2:3277659/1‑150 (MQ=255)
                                     tGATAGTATTGGATTGAAAGAGTCTTCTTTAGAAGTTCAGTTTTAGCATTCTGTCTTTCTTCTAAACCTAAATTTTTATCTTCTATCTTTTTTATAAGTTCCTTTATATTTTCTT‑CATTTTCCTCATAAATATCATATGGAATTACAGTa                                                                                                                  >  1:1059193/1‑150 (MQ=255)
                                       aTAGTATTGGATTGAAAGAGTCTTCTTTAGAAGTTCAGTTTTAGCATTCTGTCTTTCTTCTAAACCTAAATTTTTATCTTCTATCTTTTTTATAAGTTCCTTTATATTTTCTT‑CATTTTCCTCATAAATATCATATGGAATTACAGTAac                                                                                                                <  2:1418041/150‑1 (MQ=255)
                                       aTAGTATTGGATTGAAAGAGTCTTCTTTAGAAGTTCAGTTTTAGCATTCTGTCTTTCTTCTAAACCTAAATTTTTATCTTCTATCTTTTTTATAAGTTCCTTTATATTTTCTT‑CATTTTCCTCATAAATATCATATGGAATTACAGTAac                                                                                                                <  1:2812964/150‑1 (MQ=255)
                                       aTAGTATTGGATTGAAAGAGTCTTCTTTAGAAGTTCAGTTTTAGCATTCTGTCTTTATTCTAAACCTAAATTTTTATCTTCTATCTTTTTTATAAGTTCCTTTATATTTTCTT‑CATTTTCCTCATAAATATCATATGGAATTACAGTAac                                                                                                                <  2:1022311/150‑1 (MQ=255)
                                        tAGTATTGGATTGAAAGAGTCTTCTTTAGAAGTTCAGTTTTAGCATTCTGTCTTTCTTCTACACCTAACCTTTTATTTTCTATCTTTTTTATAAGTTCCTTTTTATTTTCTT‑CCTTTTCCTTATGAATGTCATCTGGAAGTACAGTagaa                                                                                                               >  2:2614761/1‑147 (MQ=255)
                                          gTATTGGATTGAAAGGGTCTTCTTTAGAAGTTCAGTTTTAGCATTCTGTCTTTCTTCTAAACCTAAATTTTTATCTTCTATCTTTTTTATAAGTTTCTTTATATTTTTTT‑CATTTTCCTCATAAATATCATATGGAATTACAGTAacatt                                                                                                             >  2:1975011/1‑148 (MQ=255)
                                          gTATTGGATTGAAAGAGTCTTCTTTAGAAGTTCAGTTTTAGCATTCTGTCTTTCTTCTAAACCTAAATTTTTATCTTCTATCTTTTTTATAAGTTCCTTTATATTTTGTT‑CATTTTCCTCATAAATATCATATGGAATTACAGTAACACt                                                                                                             >  1:3344479/1‑150 (MQ=255)
                                          gTATTGGATTGAAAGAGTCTTCTTTAGAAGTTCAGTTTTAGCATTCTGTCTTTCTTCTAAACCTAAATTTTTATCTTCTATCTTTTTTATAAGTTCCTTTATATTTTCTTTCATTTTCCTCATAAATATCATATGGAATTACAGTAacac                                                                                                              <  1:883337/150‑1 (MQ=255)
                                          gTATTGGATTGAAAGAGTCTTCTTTAGAAGTTCAGTTTTAGCATTCTGTCTTTCTTCTAAACCTAAATTTTTATCTTCTATCTTTTTTATAAGTTCCTTTATATTTTCTT‑CATTTTCCTCATACATATCATATGGAATTACAGTAACACt                                                                                                             >  1:3007178/1‑150 (MQ=255)
                                          gTATTGGATTGAAAGAGTCTTCTTTAGAAGTTCAGTTTTAGCATTCTGTCTTTCTTCTAAACCTAAATTTTTATCTTCTATCTTTTTTATAAGTTCCTTTATATTTTCTT‑CATTTTCCTCATAAATATCATATGGAATTACAGTAACACt                                                                                                             <  1:2909603/150‑1 (MQ=255)
                                            aTTGGATTGAAAGAGTCTTCTTTAGAAGTTCAGTTTTAGCATTCTGTCTTTCTTCTAAACCTAAATTTTTATCTTCTATCTTTTTTATAAGTTCCTTTATATTTTGTT‑CATTTTCCTCATAAATATCATATGGAATTACAGTAACACttt                                                                                                           >  1:1391272/1‑150 (MQ=255)
                                               ggATTGAAAGAGTCTTCTTGAGAAGTTCAGTTTTAGCATTCTGTCTGTATCCTAAACCTAAATTTTTATCTTCTATCTTTTTTATAAGTTCCTTTATATTTTCTT‑CATTTGCCTCAGAAATATCATATGGAATTACAGTAACACTTTGGa                                                                                                        <  1:1461370/150‑1 (MQ=255)
                                                gATTGAAAGAGTCTTCTTTAGAAGTTCAGTTTTAGCATTCTGTCTTTCTTCTAAACCTAAATTTTTATCTTCTATCTTTTTTATAAGTTCCTTTATATTTTCTT‑CATTTTCCTCATAAATATCATATGGAATTACAGTAACACTTTGGat                                                                                                       >  2:1589088/1‑150 (MQ=255)
                                                 aTTGAAAGAGTCTTCTTTAGAAGTTCAGTTTTAGCATTCTGTCTTTCTTCTAAACCTAAATTTTTATCTTCTATCTTTTTTATAAGTTCCTTTATATTTTTTT‑CATTTTCCTCATAAATATCATATGGAATTACAGTAACACTTTGGata                                                                                                      >  1:1335629/1‑150 (MQ=255)
                                                 aTTGAAAGAGTCTTCTTTAGAAGTTCAGTTTTAGCATTCTGTCTTTCTTCTAAACCTAAATTTTTATCTTCTATCTTTTTTATAAGTTCCTTTATATTTTTTT‑CATTTTCCTCATAAATATCATATGGAATTACAGTAACACTTTGGata                                                                                                      >  1:1783772/1‑150 (MQ=255)
                                                  ttGAAAGAGTCTTCTTTAGAAGTTCAGTTTTAGCATTCTGTCTTTCTTCTAAACCTAAATTTTTATCTTCTATCTTTTTTATAAGTTCCTTTATATTTTCTT‑CATTTTCCTCATAAATATCATATGGAATTACAGTAACACTTTGGatat                                                                                                     >  2:514200/1‑150 (MQ=255)
                                                   tGAAAGAGTCTTCTTTAGAAGTTCAGTTTTAGCATTCTGTCTTTCTTCTAAACCTAAATTTTTATCTTCTATCTTTTTTATAAGTTCCTTTATATTTTCTT‑CATTTTCCTCATAAATATCATATGGAATTACAGTAACACTTTGGATATc                                                                                                    <  1:2858412/150‑1 (MQ=255)
                                                     aaaGAGTCTTCTTTAGAAGTTCAGTTTTAGCATTCTGTCTTTCTTCTAAACCTAAATTTTTATCTTCTATCTTTTTTATAAGTTCCTTTATATTTTCTT‑CATTTTCCTCATAAATATCATATGGAATTACAGTAACACTTTGGATATccc                                                                                                  <  2:1783433/150‑1 (MQ=255)
                                                       agagTCTTCTTTAGAAGTTCAGTTTTAGCATTCTGTCTTTCTTCTAAACCTAAATTTTTATCTTCTATCTTTTTTATAAGTTCCTTTATATTTTCTT‑CATTTTCCTCATAAATATCATATGGAATTACAGTAACACTTTGGATATCCCTa                                                                                                <  2:1671266/150‑1 (MQ=255)
                                                            cttcttTAGAAGTTCAGTTTTAGCATTCTGTCTTTCTTCTAAACCTAAATTTTTATCTTCTATCTTTTTTATAAGTTCCTTTATATTTTGTT‑CATTTTCCTCATAAATATCATATGGAATTACAGTAACACTTTGGATATCCCTAAGCtt                                                                                           >  1:274426/1‑150 (MQ=255)
                                                                 ttAGAAGTTCAGTTTTAGCATTCTGTCTTTCTTCTAAACCTAAATTTTTATCTTCTATCTTTTTTATAAGTTCCTTTATATTTTCTT‑CATTTTCCTCATAAATATCATATGGAATTACAGTAACACTTTGGATATCCCTAAGCTTTAAAg                                                                                      <  1:2704570/150‑1 (MQ=255)
                                                                       gTTCAGTTTTAGCATTCTGTCTTTCTTCTAAACCTAAATTTTTATCTTCTATCTTTTTTCTAAGTTCCTTTATATTTTTTT‑CATTTTCCTCATAAATATCATATGGAATTACAGTAACACTTTGGATATCCCTAAGCTTTAAAGCATCtt                                                                                >  2:1098217/1‑150 (MQ=255)
                                                                       gTTCAGTTTTAGCATTCTGTCTTTCTTCTAAACCTAAATTTTTATCTTCTATCTTTTTTATACGTTCCTTTATATTTTCTT‑CATTTTCCTCATAAATATCATATGGAATTACAGTAACACTTTGGATATCCCTAAGCTTTAAAGCATCtt                                                                                >  2:300136/1‑150 (MQ=255)
                                                                       gTTCAGTTTTAGCATTCTGTCTTTCTTCTAAACCTAAATTTTTATCTTCTATCTTTTTTATAAGTTCCTTTATATTTTTTT‑CATTTTCCTCATAAATATCATATGGAATTACAGTAACACTTTGGATATCCCTAAGCTTTCAAGCATCtt                                                                                >  2:2333630/1‑150 (MQ=255)
                                                                       gTTCAGTTTTAGCATTCTGTCTTTCTTCTAAACCTAAATTTTTATCTTCTATCTTTTTTATAAGTTCCTTTATATTTTGTT‑CATTTTCCTCATAAATATCATATGGAATTACAGTAACACTTTGGATATCCCTAAGCTTTAAAGCATCtt                                                                                >  1:1154023/1‑150 (MQ=255)
                                                                        ttCAGTTTTAGCATTCTGTCTTTCTTCTAAACCTAAATTTTTATCTTCTATCTTTTTTATAAGTTCCTTTATATTTTTTT‑CATTTTCCTCATAAATATCATATGGAATTACAGTAACACTTTGGATATCCCTAAGCTTTAAAGCATCTtg                                                                               >  1:2548677/1‑150 (MQ=255)
                                                                         tCAGTTTTAGCATTCTGTCTTTCTTCTAAACCTAAATTTTTATCTTCTATCTTTTTTATAAGTTCCTTTATATTTTCTT‑CATTTTCCTCATAAATATCATATGGAATTACAGTAACACTTTGGATATCCCTAAGCTTTAAAGCATCTtgt                                                                              <  1:556990/150‑1 (MQ=255)
                                                                            gTTTTAGCATTCTGTCTTTCTTCTAAACCTAAATTTTTATCTTCTATCTTTTTTATAAGTTCCTTTATATTTTTTT‑CATTTTCCTCATAAATATCATATGGAATTACAGTAACACTTTGGATATCCCTAAGCTTTAAAGCATCTTGTGaa                                                                           >  2:871779/1‑150 (MQ=255)
                                                                              tttAGCATTCTGTCTTTCTTCTAAACCTAAATTTTTATCTTCTATCTTTTTTATAAGTTCCTTTATATTTTTTT‑CATTTTCCTCATAAATATCATATGGAATTACAGTAACACTTTGGATATCCCTAAGCTTTAAAGCATCTTGTGAAtt                                                                         >  2:3401703/1‑150 (MQ=255)
                                                                                tAGCATTCTGTCTTTCTTCTAAACCTAAATTTTTATCTTCTATCTTTTTTATAAGTTCCTTTATATTTTCTT‑CATTTTCCTCATAAATATCATATGGAATTACAGTAACACTTTGGATATCCCTAAGCTTTAAAGCATCTTGTGAAtttt                                                                       <  1:1079765/150‑1 (MQ=255)
                                                                                tAGCATTCTGTCTTTCTTCTAAACCTAAATTTTTATCTTCTATCTTTTTTATAAGTTCCTTTATATTTTCTT‑CATTTTCCTCATAAATATCATATGGAATTACAGTAACACTTTGGATATCCCTAAGCTTTAAAGCATCTTGTGAAtttt                                                                       <  2:2490928/150‑1 (MQ=255)
                                                                                    aTTCTGTCTTTCTTCTAAACCTAAATTTTTATCTTCTATCTTTTTTATAAGTTCCTTTATATTTTCTT‑CATTTTCCTCATAAATATCATATGGAATTACAGTAACACTTTGGATATCCCTAAGCTTTAAAGCATCTTGTGAATTTtcatc                                                                   >  1:3528779/1‑150 (MQ=255)
                                                                                    aTTCTGTCTTTCTTCTAAACCTAAATTTTTATCTTCTATCTTTTTTATAAGTTCCTTTATATTTTCTT‑CAGTTTCCTCATAAATATCATATGGAATTACAGTAACACGTTGGATATCCCTAAGCTTTAAAGCATCTTGTGAATTTtcatc                                                                   >  1:2184288/1‑150 (MQ=255)
                                                                                     ttCTGTCTTTCTTCTAAACCTAAATTTTTATCTTCTATCTTTTTTATAAGTTCCTTTATATTTTCTT‑CATTTTCCTCATAAATATCATATGGAATTACAGTAACACTTTGGATATCCCTAAGCTTTAAAGCATCTTGTGAATTTtcatca                                                                  >  2:1746938/1‑150 (MQ=255)
                                                                                      tCTGTCTTTCTTCTAAACCTAAATTTTTATCTTCTATCTTTTTTATAAGTTCCTTTATATTTTTTT‑CATTTTCCTCATAAATATCATATGGAATTACAGTAACACTTTGGATATCCCTAAGCTTTAAAGCATCTTGTGAATTTTCATCAc                                                                 >  2:2025988/1‑150 (MQ=255)
                                                                                      tCTGTCTTTCTTCTAAACCTAAATTTTTATCTTCTATCTTTTTTATAAGTTCCTTTATATTTTCTT‑CATTTTCCTCATAAATATCATATGGAATTACAGTAACACTTTGGATATCCCTAAGCTTTAAAGCATCTTGTGAATTTTCATCAc                                                                 <  2:127993/150‑1 (MQ=255)
                                                                                      tCTGTCTTTCTTCTAAACCTAAATTTTTATCTTCTATCTTTTTTATAAGTTCCTTTATATTTTCTT‑CATTTTCCTCATAAATATCATATGGAATTACAGTAACACTTTGGATATCCCTAAGCTTTAAAGCATCTTGTGAAGTTTCAGCAc                                                                 <  2:791076/150‑1 (MQ=255)
                                                                                           ctttcttCTAAACATAAATTTTTATCTTCTATCTTTTTTATAAGTTCCTTTATATTCTTTT‑CATTTTCCTCATAAATATCATATGGAATTACAGTAACACTTTGGATATCCCTAAGCTTTAAAGCATCTTGTGAATTTTCATCACTAttt                                                            >  1:3111214/1‑150 (MQ=255)
                                                                                               cttctAAACCTAAATTTTTATCTTCTATCTTTTTTATAAGTTCCTTTATATTTTTTT‑CATTTTCCTCATAAATATCATATGGAATTACAGTAACACTTTGGATATCCCTAAGCTTTAAAGCATCTTGTGAATTTTCATCACTATTTaaaa                                                        >  2:2517289/1‑150 (MQ=255)
                                                                                                     aaCCTAAATTTTTCTCTTCTATCTTTTTTATAAGTTCCTTTATATTTTCTT‑CATTTTCCTCATAAATATCATATGTAATTACAGTAACACTTTGGATATCCCTAAGCTTTAAAGCATCTTGTGAATTTTCATCACTATTTAAAATTCttt                                                  <  2:2274806/150‑1 (MQ=255)
                                                                                                       ccTAAATTTTTATCTTCTATCTTTTTTATAAGTTCCTTTATATTTTCTT‑CATTTTCCTCATAAATATCATATGGAATTACAGTAACACTTTGGATATCCCTAAGCTTTAAAGCATCTTGTGAATTTTCATCACTATTTAAAATTCTTTgg                                                >  2:3215714/1‑150 (MQ=255)
                                                                                                       ccTAAATTTTTATCTTCTATCTTTTTTATAAGTTCCTTTATATTTTCTT‑CATTTTCCTCATAAATATCATATGGAATTACAGTAACACTTTGGATATCCCTAAGCTTTAAAGCATCTTGTGAATTTTCATCACTATTTAAAATTCTTTgg                                                <  2:3151150/150‑1 (MQ=255)
                                                                                                        cTAAATTTTTATCTTCTATCTTTTTTATAAGTTCCTTTATATTTTCTT‑CATTTTCCTCATAAATATCATATGGAATTACAGTAACACTTTGGATATCCCTAAGCTTTAAAGCATCTTGTGAATTTTCATCACTATTTAAAATTCTTTGGt                                               >  1:849761/1‑150 (MQ=255)
                                                                                                         tAAATTTTTATCTTCTATCTTTTTTATAAGTTCCTTTATATTTTCTT‑CATTTTCCTCATAAATATCATATGGAATTACAGTAACACTTTGGATATCCCTAAGCTTTAAAGCATCTTGTGAATTTTCATCACTATTTAAAATTCTTTGGTa                                              >  2:2825169/1‑150 (MQ=255)
                                                                                                          aaaTTTTTATCTTCTATCTTTTTTATAAGTTCCTTTATATTTTTTT‑CATTTTCCTCATAAATATCATATGGAATTACAGTAACACTTTGGATATCCCTAAGCTTTAAAGCATCTTGTGAATTTTCATCACTATTTAAAATTCTTTGGTAt                                             >  1:738665/1‑150 (MQ=255)
                                                                                                                  aTCTTCTATCTTTTTTATAAGTTCCTTTATATTTTCTT‑CATTTTCCTCATAAATATCATATGGAATTACAGTAACACTTTGGATATCCCTAAGCTTTAAAGCATCTTGTGAATTTTCATCACTATTTAAAATTCTTTGGTATTTTTCATa                                     <  1:507990/150‑1 (MQ=255)
                                                                                                                    cttctATCTTTTTTATAAGTTCCTTTATATTTTCTT‑CATTTTCCTCATAAATATCATATGGAATTACAGTAACACTTTGGATATCCCTAAGCTTTAAAGCATCTTGTGAATTTTCATCACTATTTAAAATTCTTTGGTATTTTTCATAAg                                   <  1:3030193/150‑1 (MQ=255)
                                                                                                                     ttctATCTTTTTTATAAGTTCCTTTATATTTTCTT‑CATTTTCCTCATAAATATCATATGGAATTACAGTAACACTTTGGATATCCCTAAGCTTTAAAGCATCTTGTGAATTTTCATCACTATTTAAAATTCTTTGGTATTTTTCATAAGc                                  >  2:1336631/1‑150 (MQ=255)
                                                                                                                          tCTTTTTTATAAGTTCCTTTATATTTTTTT‑CATCTTCCTCATAAATATCATATGGAATTACAGTCACACTTTTGATATCCCTCAACTTTCAAACATCTTGTGAATTTTCCTCCCTATTTCAAATTCTTTTGTCTTTTTcctcaacttttt                             >  2:310192/1‑139 (MQ=255)
                                                                                                                           cTTTTTTATAAGTTCCTTTATATTTTCTT‑CATTTTCCTCATAAATATCATATGGAATTACAGTAACACTTTGGATATCCCTAAGCTTTAAAGCATCTTGTGAATTTTCATCACTATTTAAAATTCTTTGGTATTTTTCATAAGCTTTTTc                            >  2:3551130/1‑150 (MQ=255)
                                                                                                                            ttttttATAAGTTCCTTTATATTTTCTT‑CATTTTCCTCATAAATATCATATGGAATTACAGTAACACTTTGGATATCCCTAAGCTTTAAAGCATCTTGTGAATTTTCATCACTATTTAAAATTCTTTGGTATTTTTCATAAGCTTTTTCa                           <  2:2695218/150‑1 (MQ=255)
                                                                                                                             tttttATAAGTTCCTTTATATTTTCTT‑CATTTTCCTCATAAATATCATATGGAATTACAGTAACACTTTGGATATCCCTAAGCTTTAAAGCATCTTGTGAATTTTCATCACTATTTAAAATTCTTTGGTATTTTTCATAAGCTTTTTCaa                          <  1:3289468/150‑1 (MQ=255)
                                                                                                                             tttttATAAGTTCCTTTATATTTTCTT‑CATTTTCCTCATAAATATCATATGGAATTACAGTAACACTTTGGATATCCCTAAGCTTTAAAGCATCTTGTGAATTTTCATCACTATTTAAAATTCTTTGGTATTTTTCATAAGCTTTTTCaa                          <  1:1254929/150‑1 (MQ=255)
                                                                                                                              ttttATAAGTTCCTTTATATTTTGTT‑CATTTTCCTCATAAATATCATATGGAATTACAGTAACACTTTGGATATCCCTAAGCTTTAAAGCATCTTGTGAATTTTCATCACTATTTAAAATTCTTTGGTATTTTTCATAAGCTTTTTCaaa                         >  1:448075/1‑150 (MQ=255)
                                                                                                                              ttttATAAGTTCCTTTATATTTTCTT‑CATTTTCCTCATAAATATCATATGGAATTACAGTAACACTTTGGATATCCCTAAGCTTTAAAGCATCTTGTGAATTTTCATCACTATTTAAAATTCTTTGGTATTTTTCATAAGCTTTTTCaaa                         <  1:776314/150‑1 (MQ=255)
                                                                                                                               tttATAAGTTCCTTTATATTTTCTT‑CATTTTCCTCATAAATATCATATGGAATTACAGTAACACTTTGGATATCCCTAAGCTTTAAAGCATCTTGTGAATTTTCATCACTATTTAAAATTCTTTGGTATTTTTCATAAGCTTTTTCAAAt                        <  1:1897935/150‑1 (MQ=255)
                                                                                                                               tttATAAGTTCCTTTATATTTTCTT‑CATTTTCCTCATAAATATCATATGGAATTACAGTAACACTTTGGATATCCCTAAGCTTTAAAGCATCTTGTGAATTTTCATCACTATTTAAAATTCTTTGGTATTTTTCATAAGCTTTTTCAAAt                        <  2:2750888/150‑1 (MQ=255)
                                                                                                                                ttATAAGTTCCTTTATATTTTTTT‑CATTTTCCTCATAAATATCATATGGAATTACAGTAACACTTTGGATATCCCTAAGCTTTAAAGCATCTTGTGAATTTTCATCACTATTTAAAATTCTTTGGTATTTTTCATAAGCTTTTTCAAAtt                       >  2:417806/1‑150 (MQ=255)
                                                                                                                                     aGTTCCTTTATATTTTCTT‑CATTTTCCTCATAAATATCATATGGAATTACAGTAACACTTTGGATATCCCTAAGCTTTAAAGCATCTTGTGAATTTTCATCACTATTTAAAATTCTTTGGTATTTTTCATAAGCTTTTTCAAATtcatca                  <  2:737161/150‑1 (MQ=255)
                                                                                                                                          cTTTATATTTTTTT‑CATTTTCCTCATAAATATCATATGGAATTACAGTAACACTTTGGATATCCCTAAGCTTTAAAGCATCTTGTGAATTTTCATCACTATTTAAAATTCTTTGGTATTTTTCATAAGCTTTTTCAAATTCATCAACaaa             >  1:1972587/1‑150 (MQ=255)
                                                                                                                                            ttATATTTTCTT‑CATTTTCCTCATAAATATCATATGGAATTACAGTAACACTTTGGATATCCCTAAGCTTTAAAGCATCTTGTGAATTTTCATCACTATTTAAAATTCTTTGGTATTTTTCATAAGCTTTTTCAAATTCATCAACAAAAt           >  1:1535159/1‑150 (MQ=255)
                                                                                                                                               tatTTTCTT‑CATTTTCCTCATAAATATCATATGGAATTACAGTAACACTTTGGATATCCCTAAGCTTTAAAGCATCTTGTGAATTTTCATCACTATTTAAAATTCTTTGGTATTTTTCATAAGCTTTTTCAAATTCATCAACAAAATTAc        >  2:2403296/1‑150 (MQ=255)
                                                                                                                                                atTTTCTT‑CATTTTCCTCATAAATATCATATGGAATTACAGTAACACTTTGGATATCCCTAAGCTTTAAAGCATCTTGTGAATTTTCATCACTATTTAAAATTCTTTGGTATTTTTCATAAGCTTTTTCAAATTCATCAACAAAATTAct       <  1:2676799/150‑1 (MQ=255)
                                                                                                                                                 ttttCTT‑CATTTTCCTCATAAATATCATATGGAATTACAGTAACACTTTGGATATCCCTAAGCTTTAAAGCATCTTGTGAATTTTCATCACTATTTAAAATTCTTTGTTATTTTTCATAAGCTTTTTCAAATTCATCAACAAAATTActt      >  1:2615991/1‑150 (MQ=255)
                                                                                                                                                 ttttCTT‑CATTTTCCTCATAAATATCATATGGAATTACAGTAACACTTTGGATATCCCTAAGCTTTAAAGCATCTTGTGAATTTTCATCACTATTTAAAATTCTTTGGTATTTTTCATAAGCTTTTTCAAATTCATCAACAAAATTActt      <  1:3410014/150‑1 (MQ=255)
                                                                                                                                                   ttctt‑cATTTTCCTCATAAATATCATATGGAATTACAGTAACACTTTGGATATCCCTAAGCTTTAAAGCATCTTGTGAATTTTCATCACTATTTAAAATTCTTTGGTATTTTTCATAAGCTTTTTCAAATTCATCAACAAAATTActttc    <  1:1356463/150‑1 (MQ=255)
                                                                                                                                                   ttctt‑cATTTTCCTCATAAATATCATATGGAATTACAGTAACACTTTGGATATCCCTAAGCTTTAAAGCATCTTGTGAATTTTCATCACTATTTAAAATTCTTTGGTATTTTTCATAAGCTTTTTCAAATTCATCAACAAAATTActttc    <  1:1650003/150‑1 (MQ=255)
                                                                                                                                                   ttctt‑cATTTTCCTCATAAATATCATATGGAATTACAGTAACACTTTGGATATCCCTAAGCTTTAAAGCATCTTGTGAATTTTCATCACTATTTAAAATTCTTTGGTATTTTTCATAAGCTTTTTCAAATTCATCAACAAAATTActttc    >  1:1999583/1‑150 (MQ=255)
                                                                                                                                                     ctt‑cATTTTCCTCATAAATATCATATGGAATTACAGTAACACTTTGGATATCCCTAAGCTTTAAAGCATCTTGTGAATTTTCATCACTATTTAAAATTCTTTGGTATTTTTCATAAGCTTTTTCAAATTCATCAACAAAATTActttctt  >  2:1700811/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
TTTACCTTTTAAAATTTCACTTATATACTTACTTAACTGATAGTATTGGATTGAAAGAGTCTTCTTTAGAAGTTCAGTTTTAGCATTCTGTCTTTCTTCTAAACCTAAATTTTTATCTTCTATCTTTTTTATAAGTTCCTTTATATTTTCTT‑CATTTTCCTCATAAATATCATATGGAATTACAGTAACACTTTGGATATCCCTAAGCTTTAAAGCATCTTGTGAATTTTCATCACTATTTAAAATTCTTTGGTATTTTTCATAAGCTTTTTCAAATTCATCAACAAAATTACTTTCTT  >  CP028101/1846982‑1847280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑