Predicted mutation
evidence seq id position mutation annotation gene description
RA Xen14_191 226 2 bp→GA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Xen14_1912260AG83.9% 1134.9 / 167.9 636intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base A (51/51);  new base G (253/278);  total (307/329)
Fisher's exact test for biased strand distribution p-value = 6.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*Xen14_1912270GA83.7% 1120.5 / 175.7 636intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base G (53/51);  new base A (254/278);  total (307/329)
Fisher's exact test for biased strand distribution p-value = 5.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 636 total aligned reads displayed.

CTTGATTGTATGCCTGCGATCAATGCCAAACGTGTTTACCGGATCATGAACCGCCCCGGGAATCCTGGAGACTAAACTTCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAACGGGCAGTC‑CGTATGGTTCTGGAAAGTCAGAGCGAATATGACTCACAATGGGCGACAATTTGTTCCATTGCTCCAAAGATTGGCTGTACGCCGGAGACTCTGCGTGTCTGGGTTCGCCAGCATGAGCGGGATACCGGGGGCGGTGATGGAGGGC  >  Xen14_191/82‑374
                                                                                                                                                ||                                                                                                                                                    
            ccTGCGATCAATGCCAAACGTGTTTACCGGATCATGAACCGCCCCGGGAATCCTGGAGACTAAACTTCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAACGGGCAGTC‑CGTATGGTTCTGGa                                                                                                                                     <  1:2008533/150‑1 (MQ=255)
               gCGATCAATGCCAAACGTGTTTACCGGATCATGAACCGCCCCGGGAATCCTGGAGACTAAACTTCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAACGGGCAGTC‑CGTATGGTTCTGGAAAg                                                                                                                                  <  1:645985/150‑1 (MQ=255)
               gCGATCAATGCCAAACGTGTTTACCGGATCATGAACCGCCCCGGGAATCCTGGAGACTAAACTTCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAACGGGCAGTC‑CGTATGGTTCTGGAAAg                                                                                                                                  <  1:1430124/150‑1 (MQ=255)
                               taccaggaggaaccgtGAACCGCCCCGGGAATCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGAc                                                                                                                  <  1:43483/135‑1 (MQ=14)
                                ccagagcctgcaactGAACCGCCCCGGGAATCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTAAGGGCGAATATGACt                                                                                                                 >  1:4026540/15‑150 (MQ=14)
                                agtttttccagtattGAACCGCCCCGGGAATCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACt                                                                                                                 >  1:3315942/15‑150 (MQ=14)
                                 ccaggaggaaccgtGAACCGCCCCGGGAATCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTc                                                                                                                >  1:1562022/14‑150 (MQ=14)
                                  cttcaatctgcttGAACCGCCCCGGGAATCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTca                                                                                                               >  1:1296713/13‑150 (MQ=14)
                                   ttACCGGATCATGAACCGCCCCGGGAATCCTGGAGACTAAACTTCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAACGGGCAGTC‑CGTATGGTTCTGGAAAGTCAGAGCGAATATGACTcac                                                                                                              >  1:1927903/1‑150 (MQ=255)
                                    tACCGGATCATGAACCGCCCCGGGAATCCTGGAGACTAAACTTCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAACGGGCAGTC‑CGTATGGTTCTGGAAAGTCAGAGCGAATATGACTcaca                                                                                                             <  1:692245/150‑1 (MQ=255)
                                      ttatgacctGAACCGCCCCGGGAATCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTcacagt                                                                                                           <  1:3592168/142‑3 (MQ=17)
                                      cctgcaactGAACCGCCCCGGGAATCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTcacagt                                                                                                           >  1:2229708/9‑148 (MQ=17)
                                      ccGGATCATGAACCGCCCCGGGAATCCTGGAGACTAAACTTCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAACGGGCAGTC‑CGTATGGTTCTGGAAAGTCAGAGCGAATATGACTCACAAt                                                                                                           >  1:1081654/1‑150 (MQ=255)
                                       ctgcaactGAACCGCCCCGGGAATCCTTGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTca                                                                                                               <  1:4125506/138‑1 (MQ=17)
                                          gtattGAACCGCCCCGGGAATCCTGGAGACTAAACTCCCTGAGGAAGAGGTAAAAAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTggtc                                                                                                       >  1:3975592/5‑148 (MQ=17)
                                          aTCATGAACCGCCCCGGGAATCCTGGAGACTAAACTTCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAACGGGCAGTC‑CGTATGGTTCTGGAAAATCAGAGCGAATATGACTCACAATGGGc                                                                                                       >  1:2775169/1‑150 (MQ=255)
                                           tattGAACCGCCCCGGGAATCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACGGTGGGCg                                                                                                      >  1:1152183/4‑150 (MQ=17)
                                           tCATGAACCGCCCCGGGAATCCTGGAGACTAAACTTCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCTTTTTTCCCCCGAAGTCCGTCAACGGGCAGTC‑CGTATGGTTCTGGAAAGTCAGAGCGAATATGACTCACAATGGGCg                                                                                                      <  1:2999048/150‑1 (MQ=255)
                                             ctGAACCGCCCCGGGAATCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCggc                                                                                                    <  1:3657253/149‑3 (MQ=18)
                                               gAACCGCCCCGGGAATCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCaa                                                                                                  >  1:1588036/1‑150 (MQ=18)
                                                aaCCTCCCCGGGAATCCTGGAGACTAAACTTCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAACGGGCAGTC‑CGTATGGTTCTGGAAAGTCAGAGCGAATATGACTCACAATGGGCGACAAt                                                                                                 >  1:1636611/1‑150 (MQ=255)
                                                aaCCGCCCCGGGAATCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAAt                                                                                                 <  1:3635477/150‑1 (MQ=18)
                                                  ccGCCCCGGGAATCCTGGAGACTAAACTTCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAACGGGCAGTC‑CGTATGGTTCTGGAAAGTCAGAGCGAATATGACTCACAATGGGCGACAAttt                                                                                               <  1:540769/150‑1 (MQ=255)
                                                  ccGCCCCGGGAATCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAAttt                                                                                               <  1:225430/150‑1 (MQ=18)
                                                   cGCCCCGGGAATCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAGCAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGACGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTg                                                                                              <  1:1222593/150‑1 (MQ=18)
                                                    gCCCCGGGAATCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGt                                                                                             <  1:1123221/150‑1 (MQ=18)
                                                     ccccGGGAATCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGtt                                                                                            >  1:3328600/1‑150 (MQ=18)
                                                     ccccGGGAATCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGtt                                                                                            >  1:2575678/1‑150 (MQ=18)
                                                      cccGGGAATCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTc                                                                                           >  1:368535/1‑150 (MQ=18)
                                                       ccGGGAATCCTGGAGACTAAACTTCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAACGGGCAGTC‑CGTATGGTTCTGGAAAGTCAGAGCGAATATGACTCACAATGGGCGACAATTTGTTcc                                                                                          <  1:384454/150‑1 (MQ=255)
                                                       ccGGGAATCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTcc                                                                                          <  1:44744/150‑1 (MQ=21)
                                                         gggAATCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCAt                                                                                        >  1:1044739/1‑150 (MQ=21)
                                                             aTCCTGGAGGCTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGcc                                                                                    <  1:1617305/150‑2 (MQ=21)
                                                              tCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGGTGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGccc                                                                                   >  1:57511/1‑148 (MQ=21)
                                                                cTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCa                                                                                 >  1:3475368/1‑150 (MQ=21)
                                                                cTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCa                                                                                 <  1:1461434/150‑1 (MQ=21)
                                                                  ggAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCTATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTg                                                                                              >  1:2309973/1‑135 (MQ=21)
                                                                  ggAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCaaa                                                                               <  1:3224442/150‑1 (MQ=21)
                                                                   gagaCTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCTCCAAAg                                                                              <  1:4095319/150‑1 (MQ=25)
                                                                   gagaCTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAg                                                                              <  1:307794/150‑1 (MQ=21)
                                                                    agaCTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGa                                                                             <  1:4235055/150‑1 (MQ=21)
                                                                     gaCTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGAt                                                                            >  1:818246/1‑150 (MQ=21)
                                                                      aCTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGAtt                                                                           <  1:1758653/150‑1 (MQ=25)
                                                                       cTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTg                                                                          <  1:476684/150‑1 (MQ=25)
                                                                       cTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTg                                                                          >  1:1812083/1‑150 (MQ=25)
                                                                         aaaCTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGc                                                                        <  1:3739840/150‑1 (MQ=25)
                                                                           actcccTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGNCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTg                                                                      <  1:3895852/146‑1 (MQ=25)
                                                                            ctcccTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGt                                                                     >  1:173706/4‑150 (MQ=25)
                                                                             tcccTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTa                                                                    <  1:2205477/148‑1 (MQ=25)
                                                                              cccTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAGGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGATTCACAGTGGGCGGCAATTTGTTCCGTTGCCCCAAAGATTGGCTGTAc                                                                   <  1:126760/149‑1 (MQ=21)
                                                                                 tGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTAcacc                                                                >  1:2670372/1‑147 (MQ=37)
                                                                                 tGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGC‑GACTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTAcacc                                                                >  1:606973/1‑147 (MQ=25)
                                                                                 tGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAACGGGCAGTC‑CGTATGGTTCTGGAAAGTCAGAGCGAATATGACTCACAATGGGCGACAATTTGTTCCATTGCTCCAAAGATTGGCTGTACGcc                                                                >  1:1320430/1‑150 (MQ=255)
                                                                                    gaAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCt                                                                       >  1:3495660/1‑140 (MQ=37)
                                                                                    gaAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCt                                                                       >  1:3160381/1‑140 (MQ=37)
                                                                                    gaAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGAGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGga                                                             >  1:23662/1‑150 (MQ=25)
                                                                                     aaaGAGGTAAACAGGATGACTAAAAATACTCTTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGgag                                                            <  1:2710631/150‑1 (MQ=255)
                                                                                     aaaGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGgag                                                            <  1:1005238/150‑1 (MQ=37)
                                                                                       agagGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGAc                                                          <  1:4143955/150‑1 (MQ=37)
                                                                                         agGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGActc                                                        >  1:2130906/1‑150 (MQ=255)
                                                                                          ggTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGActct                                                       >  1:3045447/1‑150 (MQ=255)
                                                                                          ggTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCAGAGActct                                                       >  1:2474793/1‑150 (MQ=255)
                                                                                            tAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGc                                                     <  1:14191/150‑1 (MQ=255)
                                                                                            tAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGc                                                     <  1:630684/150‑1 (MQ=255)
                                                                                             aaaCAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGCCCGTCAACGGGCAGTC‑CGTATGGTTCTGGAAAGTCAGAGCGAATATGACTCACAATGGGCGACAATTTGTTCCATTGCTCCAAAGATTGGCTGTACGCCGGAGACTCTGCg                                                    <  1:2349127/150‑1 (MQ=255)
                                                                                              aaCAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCgt                                                   >  1:1830662/1‑150 (MQ=255)
                                                                                              aaCAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCgt                                                   >  1:878223/1‑150 (MQ=255)
                                                                                               aCAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCgtg                                                  <  1:3787085/150‑1 (MQ=255)
                                                                                               aCAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAACGGGCAGTC‑CGTATGGTTCTGGAAAGTCAGAGCGAATATGACTCACAATGGGCGACAATTTGTTCCATTGCTCCAAAGATTGGCTGTACGCGGGAGACTCTGCgtg                                                  <  1:279564/150‑1 (MQ=255)
                                                                                                 aGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTc                                                <  1:1867616/150‑1 (MQ=255)
                                                                                                 aGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTc                                                <  1:178021/150‑1 (MQ=255)
                                                                                                 aGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTc                                                <  1:1533558/150‑1 (MQ=255)
                                                                                                   gATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTg                                              >  1:2893102/1‑150 (MQ=255)
                                                                                                     tGACTAAAGATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTggg                                            <  1:2073294/150‑1 (MQ=255)
                                                                                                     tGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTggg                                            <  1:83978/150‑1 (MQ=255)
                                                                                                       aCTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGta                                          >  1:3430010/1‑149 (MQ=255)
                                                                                                       aCTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGta                                          >  1:2169510/1‑149 (MQ=255)
                                                                                                        cTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCGATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGtac                                         >  1:2556145/1‑148 (MQ=255)
                                                                                                        cTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTTTGGGtac                                         <  1:2955567/150‑3 (MQ=255)
                                                                                                        cTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAACGGGCAGTC‑CGTATGGTTCTGGAAAGTCAGAGCGAATATGACTCACAATGGGCGACAATTTGTTCCATTGCTCCAAAGATTGGCTGTACGCCGGAGACTCTGCGTGTCTGGGTTc                                         >  1:2536622/1‑150 (MQ=255)
                                                                                                        cTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAACGGGCAGTC‑CGTATGGTTCTGGAAAGTCAGAGCGAATATGACTCACAATGGGCGACAATTTGTTCCATTGCTCCAAAGATTGGCTGTACGCCGGAGACTCTGCGTGTCTGGGTTc                                         <  1:1377024/150‑1 (MQ=255)
                                                                                                            aaaTACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCa                                     <  1:4203005/150‑1 (MQ=255)
                                                                                                            aaaTACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTCCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCg                                                    >  1:3563833/1‑135 (MQ=255)
                                                                                                             aaTACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAg                                    <  1:2093573/150‑1 (MQ=255)
                                                                                                             aaTACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGACAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAg                                    <  1:2606262/150‑1 (MQ=255)
                                                                                                               tACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCa                                  <  1:939120/150‑1 (MQ=255)
                                                                                                                aCTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATTACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCAt                                 <  1:2446819/150‑1 (MQ=255)
                                                                                                                aCTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGCTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCAt                                 <  1:134558/150‑1 (MQ=255)
                                                                                                                 cTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGTCAGCATg                                >  1:1809752/1‑150 (MQ=255)
                                                                                                                 cTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATg                                >  1:2387864/1‑150 (MQ=255)
                                                                                                                     tttttCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCg                            >  1:4051565/1‑150 (MQ=255)
                                                                                                                       tttCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCggg                          <  1:2849084/150‑1 (MQ=255)
                                                                                                                         tCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCGGGAt                        <  1:3296674/150‑1 (MQ=255)
                                                                                                                         tCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCGGGAt                        <  1:1500027/150‑1 (MQ=255)
                                                                                                                         tCCCCCGAAGTCCGTCAACGGGCAGTC‑CGTATGGTTCTGGAAAGTCAGAGCGAATATGACTCACAATGGGCGACAATTTGTTCCATTGCTCCAAAGATTGGCTGTACGCCGGAGACTCTGCGTGTCTGGGTTCGCCAGCATGAGCGGGAt                        >  1:3250071/1‑150 (MQ=255)
                                                                                                                          cccccGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGActct                                                       <  1:3369433/118‑1 (MQ=255)
                                                                                                                          cccccGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCg                            <  1:733062/145‑1 (MQ=255)
                                                                                                                          cccccGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCGGGATa                       >  1:79324/1‑150 (MQ=255)
                                                                                                                          cccccGAAGTCCGTCAACGGGCAGTC‑CGTATGGTTCTGGAAAGTCAGAGCGAATATGACTCACAATGGGCGACAATTTGTTCCATTGCTCCAAAGATTGGCTGTACGCCGGAGACTCTGCGTGTCTGGGTTCGCCAGCATGAGCg                            <  1:3544060/145‑1 (MQ=255)
                                                                                                                            cccGAAGTCCGTCAGAGGGCGATT‑CGTACGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCGGGATAcc                     >  1:2915477/1‑150 (MQ=255)
                                                                                                                                                ||                                                                                                                                                    
CTTGATTGTATGCCTGCGATCAATGCCAAACGTGTTTACCGGATCATGAACCGCCCCGGGAATCCTGGAGACTAAACTTCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAACGGGCAGTC‑CGTATGGTTCTGGAAAGTCAGAGCGAATATGACTCACAATGGGCGACAATTTGTTCCATTGCTCCAAAGATTGGCTGTACGCCGGAGACTCTGCGTGTCTGGGTTCGCCAGCATGAGCGGGATACCGGGGGCGGTGATGGAGGGC  >  Xen14_191/82‑374

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑