Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	Xen14_83	11,287	T→C	intergenic (+24/–)	Xen14_04442 → / –	hypothetical protein/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Xen14_83	11,287	0	T	C	100.0%	18.1 / NA	9	intergenic (+24/–)	Xen14_04442/–	hypothetical protein/–
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (2/7);  total (2/7)

TAATTAGCAGAGAATATGGTAAGTATCCCGGCGTGCTCGTTGAAATTTTACGTCGCGTCAATACGAGGACGCAGAAAGGGTGTGCTTTAAGCTTAACCGAAGCGTTTGATAGTTGATGACCTGCAGGCATGCAAGCTTGTGGCCGCGGCCTAGGGGGTCGAAGCTTGTATGCCTGCAGGTCGATTTTCGAGGACCCAGCTGTAATCCGG‑‑‑‑  >  Xen14_83/11148‑11356                                                                                                                                            |                                                                          tAATTAGCAGAGAATATGGTAAGTATCCCGGCGTGCTCGTTGAAATTTTACGTCGCGTCAATACGAGGACGCAGAAAGGGTGTGCTTTAAGCTTAACCGAAGCGTTTGATAGTTGATGACCTGCAGGCATGCAAGCTTgcggccgcggcc                                                                 <  1:1293272/150‑1 (MQ=255) tAATTAGCAGAGAATATGGTAAGTATCCCGGCGTGCTCGTTGAAATTTTACGTCGCGTCAATACGAGGACGCAGAAAGGGTGTGCTTTAAGCTTAACCGAAGCGTTTGATAGTTGATGACCTGCAGGCATGCAAGCTTgcggccgcggcc                                                                 <  1:2401076/150‑1 (MQ=255)          gagaATATGGTAAGTATCCCGGCGTGCTCGTTGAAATTTTACGTCGCGTCAATACGAGGACGCAGAAAGGGTGTGCTTTAAGCTTAACCGAAGCGTTTGATAGTTGATGACCTGCAGGCATGCAAGCTTGCGGCCGCGGCCTAggcggcc                                                        <  1:1289490/150‑6 (MQ=255)           agaATATGGTAAGTATCCCGGCGTGCTCGTTGAAATTTTACGTCGCGTCAATACGAGGACGCAGAAAGGGTGTGCTTTAAGCTTAACCGAAGCGTTTGATAGTTGATGACCTGCAGGCATGCAAGCTTGCGGCCGCGGCCTAggcggccg                                                       <  1:2414587/150‑7 (MQ=255)              atatGGTAAGTATCCCGGCGTGCTCGTTGAAATTTTACGTCGCGTCAATACGAGGACGCAGAAAGGGTGTGCTTTAAGCTTAACCGAAGCGTTTGATAGTTGATGACCTGCAGGCATGCAAGCTTGCGGCCGCGGCCTAGGCGGCCGAAg                                                    <  1:1724258/150‑1 (MQ=255)               tatGGTAAGTATCCCGGCGTGCTCGTTGAAATTTTACGTCGCGTCAATACGAGGACGCAGAAAGGGTGTGCTTTAAGCTTAACCGAAGCGTTTGATAGTTGATGACCTGCAGGCATGCAAGCTTGCGGCCGCGGCCTAGGCGGCCGAAGc                                                   <  1:3435568/150‑1 (MQ=255)                          tCCCGGCGTGCTCGTTGAAATTTTACGTCGCGTCAATACGAGGACGCAGAAAGGGTGTGCTTTAAGCTTAACCGAAGCGTTTGATAGTTGATGACCTGCAGGCATGCAAGCTTGCGGCCGCGGCCTAGGCGGCCGAAGCTTGCATGCCTg                                        <  1:1465412/150‑1 (MQ=255)                             ctgCGTGCTCGTTGAAATTTTACTTCGCGTCAATACGAGGACGCAGAAAGGGTGTGCTTTAAGCTTAATCGAAGCGTTTGATAGTTGATGACCTGCAGGCATGCAAGCTTGCGGCCGCGGCCTAGGCGGCCGAAGCTTGCATGCCTGCAg                                     <  1:2131184/148‑1 (MQ=255)                                       gTTGAAATTTTACGTCGCGTCAATACGAGGACGCAGAAAGGGTGTGCTTTAAGCTTAACCGAAGCGTTTGATAGTTGATGACCTGCAGGCATGCAAGCTTGCGGcc                                                                       <  1:437168/106‑1 (MQ=255)                                                    gTCGCGTCAATACGAGGACGCAGAAAGGGTGTGCTTTAAGCTTAACCGAAGCGTTTGATAGTTGATGACCTGCAGGCATGCAAGCTTGCGGCCGCGGCCTAGGCGGCCGAAGCTTGCATGCCTTCAGGTCGACTCTCGAGGATCCGGCTg              >  1:3072635/1‑150 (MQ=255)                                                                cGAGGACGCAGAAAGGGTGTGCTTTAAGCTTAACCGAAGCGTTTGATAGTTGATGACCTGCAGGCATGCAAACTTGCGGCCGCGGCCTAGGCGGCCGAAGCTTGCATGCCTGCAGGTCGACTCTGGAGGATCCGGCTGTAATCCgggcag  >  1:1075471/1‑146 (MQ=255)                                                                                                                                            |                                                                          TAATTAGCAGAGAATATGGTAAGTATCCCGGCGTGCTCGTTGAAATTTTACGTCGCGTCAATACGAGGACGCAGAAAGGGTGTGCTTTAAGCTTAACCGAAGCGTTTGATAGTTGATGACCTGCAGGCATGCAAGCTTGTGGCCGCGGCCTAGGGGGTCGAAGCTTGTATGCCTGCAGGTCGATTTTCGAGGACCCAGCTGTAATCCGG‑‑‑‑  >  Xen14_83/11148‑11356

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑