| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | Xen14_43 | 24,069 | A→C | intergenic (‑200/‑398) | traM ← / → Xen14_03555 | Relaxosome protein TraM/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Xen14_43 | 24,069 | 0 | A | C | 88.9% | 14.5 / NA | 10 | intergenic (‑200/‑398) | traM/Xen14_03555 | Relaxosome protein TraM/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (0/1); major base C (1/7); minor base T (0/1); total (1/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AATGCAAACAGGGACGCACCGCTAGCAGCGCCCCTAGCGGTATCCTATAAAAAAACACACCGCGCCGCTAGCAGCACCCCTAATATAAAATAATGTTTTTTATAAAAATAGTCAGTACCACCCCTACAAAGCGGTGTCGGCGAGTTGTTGTAGCCGCGCCGACACCGCTTTTTTAAATATCATAAAGAGAGTAAGAGAAACTAATTTTTCATAAATCTCTATTTATAAATAAAAATCAGCAAAAACTTGTTTTTGCGTGGGGTGTGGTGCTTTTGGTGGTGAGAA > Xen14_43/23927‑24211 | aatacAAACAGGGACGCACCGCTAGCAGCGCCCCAAGCGGTATCCTAGAAAAAAACACACCGCGCCGCTAGCAGCACCCCTAATATAAAATAATGTTTTTTATAAAAATAGTCAGTACCACCCCTACAAAGCGGTGTCGGCGCGttgttg < 1:4216425/146‑1 (MQ=255) cAAACAGGGACGCACCGCTAGCAGCGCCCCTAGTGGTATCCTATAAAAAAACACACCGCGCCGCTAGCAGCACCCCTAATATAAAATAATGTTTTTTATAAAAATAGTCAGTACCACCCCTACAAAGCGGTGTCGGCGCGTTGTTGTAGc < 1:3773930/150‑1 (MQ=255) ggACGCACCGCTAGCAGCGCCCCTAGTGGTATCCTATAAAAAAACACACCGCGCCGCTAGCAGCACCCCTAATATAAAATAATGTTTTTTATAAAAATAGTCAGTACCACCCCTACAAAGCGGTGTCGGCGCGTTGTTGTAGCCGCGCCg < 1:478135/150‑1 (MQ=255) ggACGCACCGCTAGCAGCGCCCCTAGCGGTATCCTATAAAAAAACACACCGCGCCGCTAGCAGCACCCCTAATATAAAATAATGTTTTTTATAAAAATAGTCAGTACCACCCCTACAAAGCGGTGTCGGCGCGTTGTTGTAGCCGCGCCg < 1:2498845/150‑1 (MQ=255) acacACCCCTACCAGCGCTCCGAGCGGTATCCGACAAAAAAACACACCGCGCCGCTAGCAGCACCCCTAATATAAAATAATGTTTTTTATAAAAATAGTCAGTACCACCCCTACAAAGCGGTGTCGGCGCGTTGTTGTAGCCGCGCCGac < 1:1919329/147‑1 (MQ=255) aCGCACCGCTAGCAGCGCCCCTAGCGGTATCCTAGAAAAAAACACACCGCGCCGCTAGCAGCACCCCTAATATAAAATAATGTTTTTTATAAAAATAGTCAGTACCACCCCTACAAAGCGGTGTCGGCGCGTTGTTGGAGCCGCGCCGac < 1:2198958/150‑1 (MQ=255) aaaaCACACCGCGCCGCGAGCATCACCCCGAGGAAAAAAGAATGTTTTTAGGAAAGAGAGTCAGTACCACCCCTAAAAGGTGGGGTGGGAGAGTGATGGTAGCCGCGCCGACACCGCTTTGTTAAATATCATAAAGAGAGTAAGAGAAAc < 1:3687470/150‑1 (MQ=255) cccTAATACAAAATAGTTTTTTTTAAAAAAGTATTCAGTCCCCCCCCCACAAAGTGGTGTCGGCGTGTTGTGGCAGCCGCGCCGACACCGCTTTTTTAAATATCATAAAGAGAGTAAGAGAAACTAATTTTTCATAAATCTCTATTtata < 1:2746808/150‑1 (MQ=255) gtgtCGGCGCGTTTTTGTAGCCGCCCCGACACCGCTTTTTTAAATATCATAAAGAGAGTAAGAGAAACTAATTTTTCATAAATCTCTATTTATAAATAAAAATCAGCAAAAACTTGTTTTTGCGTGGGGTGTGGTGCTTTTGGTGGTgag < 1:3194021/150‑1 (MQ=255) gtCGGCGCGTTGTTGTAGCCGCGCCGACACCGCTTTTTTAAATATCATAAAGAGAGTAAGAGAAACTAATTTTTCATAAATCTCTATTTATAAATAAAAATCAGCAAAAAATTGTTTTTGCGTGGGGTGTGGTGCTTTTGGTGGTGAGaa > 1:2835417/1‑150 (MQ=255) | AATGCAAACAGGGACGCACCGCTAGCAGCGCCCCTAGCGGTATCCTATAAAAAAACACACCGCGCCGCTAGCAGCACCCCTAATATAAAATAATGTTTTTTATAAAAATAGTCAGTACCACCCCTACAAAGCGGTGTCGGCGAGTTGTTGTAGCCGCGCCGACACCGCTTTTTTAAATATCATAAAGAGAGTAAGAGAAACTAATTTTTCATAAATCTCTATTTATAAATAAAAATCAGCAAAAACTTGTTTTTGCGTGGGGTGTGGTGCTTTTGGTGGTGAGAA > Xen14_43/23927‑24211 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |