BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	Xen14_202	341	A→G	intergenic (–/–)	– / –	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Xen14_202	341	0	A	G	91.4%	1053.2 / 58.9	503	intergenic (–/–)	–/–	–/–
Reads supporting (aligned to +/- strand): ref base A (21/22); new base G (184/273); total (207/296)
Fisher's exact test for biased strand distribution p-value = 3.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.19e-01

Only 100 of 503 total aligned reads displayed.

AACGCAGTGCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACAGCGCGTATACGATGAAAATCACAAGGTATACGGTGTGCGTAAAGTCTGGCGTCAGTTGTTACGGGAAGGTATCAGAGTGGCCAGATGCACTGTGGCACGTCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCGTTGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  Xen14_202/196‑421

     aGTGCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCacg                                                                                        >  1:1934427/1‑148 (MQ=255)

      gTGCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATgg                                                                                       <  1:3566072/150‑1 (MQ=255)

      gTGCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATgg                                                                                       >  1:1849995/1‑150 (MQ=255)

      gTGCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATgg                                                                                       <  1:4200162/150‑1 (MQ=255)

       tGCCCGTGCGCAGCGCGATGACTTGCTGAAGAAAGAGATACTGCACGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGc                                                                                      <  1:1152408/150‑1 (MQ=255)

        gCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCg                                                                                     >  1:1616704/1‑150 (MQ=255)

         cccGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCgg                                                                                    <  1:2903196/150‑1 (MQ=255)

            gTGCTCAGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCACCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTa                                                                                 >  1:754071/1‑150 (MQ=255)

               cgcAGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATgg                                                                              <  1:2564373/150‑1 (MQ=255)

                 cAGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGa                                                                            <  1:2206355/150‑1 (MQ=255)

                 cAGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGGACAGTGGCGCGCCTCATGGCGGTTATGGGa                                                                            <  1:3124657/150‑1 (MQ=255)

                     gcgATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTg                                                                        <  1:946820/150‑1 (MQ=255)

                     gcgATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTg                                                                        >  1:4251304/1‑150 (MQ=255)

                      cgATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGc                                                                       <  1:3486944/150‑1 (MQ=255)

                       gATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGcc                                                                      <  1:4123570/150‑1 (MQ=255)

                           aCTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGCAGGAATCAGGGTAGCCAGATGTACAGTGACGCGCCTAATGGCGGTTATGGGACTCGCCGgtg                                                                  >  1:2777117/1‑150 (MQ=255)

                            cTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTTAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCGCATGGCGGTTATGGGACTTGCCGgtgt                                                                 <  1:4228411/150‑1 (MQ=255)

                            cTGGCTGAAGAAAGAGATACAGCGCGTATACGATGAAAATCACAAGGTATACGGTGTGCGTAAAGTCTGGCGTCAGTTGTTACGGGAAGGTATCAGAGTGGCCAGATGCACTGTGGCACGTCTCATGGCGGTTATGGGACTTGCCGgtgt                                                                 >  1:4107157/1‑150 (MQ=255)

                             tGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGtt                                                                >  1:1565542/1‑150 (MQ=255)

                             ggatctgatttcaggcgttgggtgtggatcactattgcaccgttcgtgacaGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGtt                                                                <  1:1452923‑M2/99‑1 (MQ=255)

                              ggCTGAAGAAAAAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTTCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTtc                                                               <  1:1121197/150‑1 (MQ=255)

                               gCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTtct                                                              >  1:2518869/1‑150 (MQ=255)

                                cTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTtctc                                                             >  1:1297985/1‑150 (MQ=255)

                                cTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGATTATGGGACTTGCCGGTGTtctc                                                             >  1:2234278/1‑150 (MQ=255)

                                 tGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTcc                                                            <  1:3258841/150‑1 (MQ=255)

                                   aagaaagaGATACTGCGCGTATACGATGGGAGTCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCgg                                                          <  1:2596995/150‑1 (MQ=25)

                                    agaaagaGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCggg                                                         >  1:1753393/1‑150 (MQ=25)

                                       aagaGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTa                                                      >  1:613189/1‑150 (MQ=25)

                                       aagaGATACAGCGCGTATACGATGAAAATCACAAGGTATACGGTGTGCGTAAAGTCTGGCGTCAGTTGTTACGGGAAGGTATCAGAGTGGCCAGATGCACTGTGGCACGTCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTa                                                      >  1:201916/1‑150 (MQ=255)

                                          ggcgttgggtgtggatcactattgcaccgttcgtgacaGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTaaaa                                                   >  1:2341743‑M2/39‑150 (MQ=255)

                                            aTACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAg                                                 <  1:1371107/150‑1 (MQ=21)

                                            aTACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGCGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAg                                                 <  1:3999681/150‑1 (MQ=21)

                                             tactgcgcGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAgg                                                <  1:2711398/146‑1 (MQ=21)

                                             tactgcgcGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAgg                                                <  1:52955/146‑1 (MQ=21)

                                             tactgcgcGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAgg                                                <  1:2476727/146‑1 (MQ=21)

                                             tactgcgcGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAgg                                                <  1:883252/146‑1 (MQ=21)

                                             tACAGCGCGTATACGATGAAAATCACAAGGTATACGGTGTGCGTAAAGTCTGGCGTCCGTTGTTACGGGATGGTATCAGAGTGGCAAGATGCACTGTGGCACGTCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAgg                                                >  1:3204943/1‑150 (MQ=255)

                                               ctgcgcGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTc                                              <  1:231066/148‑1 (MQ=21)

                                               ctgcgcGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTc                                              <  1:1178657/148‑1 (MQ=21)

                                                 gcgcGTATACGATGGGAATCATCAGGTATATGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCg                                            <  1:2819899/150‑1 (MQ=21)

                                                 gcgcGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCg                                            <  1:2270466/150‑1 (MQ=21)

                                                 gcgcGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCg                                            <  1:2553690/150‑1 (MQ=21)

                                                 gcgcGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCg                                            <  1:4030068/150‑1 (MQ=21)

                                                    gtggatcactattgcaccgttcgtgacaGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATNGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTAc                                         <  1:3895284‑M2/122‑1 (MQ=255)

                                                    gtggatcactattgcaccgttcgtgacaGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTAc                                         >  1:2102865‑M2/29‑150 (MQ=255)

                                                    cgTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTAc                                         >  1:906009/1‑150 (MQ=21)

                                                      tataCGATGAAAATCACAAGGTATACGGTGTGCGTAAAGTCTGGCGTCAGTTGTTACGGGAAGGTATCAGAGTGGCCAGATGCACTGTGGCACGTCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGa                                       >  1:3515213/1‑150 (MQ=255)

                                                       ataCGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGAAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGAc                                      <  1:2433861/150‑1 (MQ=18)

                                                          cgatgggaatcatcgggTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCAt                                   >  1:1508872/16‑150 (MQ=18)

                                                            atgggaatcatcaGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCGTGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCa                                 <  1:1707728/138‑1 (MQ=18)

                                                               ggaatcatcaGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGcc                              <  1:3168177/141‑1 (MQ=18)

                                                                gaatcatcaGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCTGGGTAAAAAGGTCCGTACGACCATCAGCCg                             >  1:1870767/9‑150 (MQ=18)

                                                                gaatcatcaGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCg                             >  1:1029039/9‑150 (MQ=18)

                                                                aaatcatcaGGTATACGGTGTGCGTAAAGTCTGGAGTCAGCTGTTACGGGAAGGAATCAGGGTAGCAAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTCCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCg                             >  1:2680321/9‑150 (MQ=18)

                                                                aaaTCACAAGGTATACGGTGTGCGTAAAGTCTGGCGTCAGTTGTTACGGGAAGGTATCAGAGTGGCCAGATGCACTGTGGCACGTCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCg                             >  1:3931280/1‑150 (MQ=255)

                                                                  atcatcaGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGa                           >  1:3148108/7‑150 (MQ=18)

                                                                     cgttcgtgacaGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAg                        <  1:869946‑M2/139‑1 (MQ=255)

                                                                     cgttcgtgacaGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGAGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAg                        >  1:3680268‑M2/12‑150 (MQ=255)

                                                                     atcaGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAATGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCAGCAGCCGGAAAg                        <  1:3220743/147‑1 (MQ=18)

                                                                     atcaGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAg                        <  1:1802176/147‑1 (MQ=18)

                                                                     atcaGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAg                        <  1:2622218/147‑1 (MQ=18)

                                                                     atcaGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAg                        >  1:586641/4‑150 (MQ=18)

                                                                     atcaGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAg                        <  1:1687777/147‑1 (MQ=18)

                                                                        aGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCg                     <  1:3869331/150‑1 (MQ=18)

                                                                        aGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCAGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCg                     <  1:3024665/150‑1 (MQ=18)

                                                                         ggTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACCTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCGt                    >  1:3355817/1‑150 (MQ=18)

                                                                          gtgacaGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGTCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCGtt                   >  1:3829355‑M2/7‑150 (MQ=255)

                                                                          gTATACGGTGTGCGTAAAGTCTGGCGTCAGTTGTTACGGGAAGGTATCAGAGTGGCCAGATGCACTGTGGCACGTCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCGtt                   >  1:3447582/1‑150 (MQ=255)

                                                                           tataCGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCGTTg                  <  1:4083258/150‑1 (MQ=18)

                                                                           tataCGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCGTTg                  <  1:506086/150‑1 (MQ=18)

                                                                           tataCGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCGTTg                  >  1:1523173/1‑150 (MQ=18)

                                                                            gacaGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTTATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCGTTGc                 <  1:68128‑M2/146‑1 (MQ=255)

                                                                            gacaGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCGTTGc                 >  1:1887822‑M2/5‑150 (MQ=255)

                                                                            gacaGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCGTTGc                 <  1:563799‑M2/146‑1 (MQ=255)

                                                                            gacaGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCGTTGc                 <  1:460624‑M2/146‑1 (MQ=255)

                                                                            gacaGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCGTTGc                 >  1:3301840‑M2/5‑150 (MQ=255)

                                                                            ataCGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCGTTGc                 <  1:3040851/150‑1 (MQ=18)

                                                                              aCGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCGTTGccg               >  1:3506123/1‑148 (MQ=18)

                                                                              aCGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCGTTGccg               <  1:200253/150‑3 (MQ=18)

                                                                               cGGTGTGCGTAAAGTCTGGCGTCAGTTGTTACGGGAAGGTATCAGAGTGGCCAGATGCACTGTGGCACGTCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCGTTGccgc              >  1:3594239/1‑147 (MQ=255)

                                                                                ggTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCGTTGccgca             <  1:3400825/150‑5 (MQ=17)

                                                                                 gtgtGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTAc                                         <  1:2392494/121‑1 (MQ=18)

                                                                                  tgtgCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCGTTGccgcagg           >  1:3732644/1‑144 (MQ=17)

                                                                                  tgtgCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCGTTGccgcagg           <  1:107048/150‑7 (MQ=17)

                                                                                  tgtgCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCGTTGccgcagg           >  1:2985530/1‑144 (MQ=17)

                                                                                   gtgCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCGTTGccgcaggc          <  1:572353/150‑8 (MQ=17)

                                                                                   gtgCGTAAAGTCTGGAGTAAGTTGTTAAGGGAAGGAGTCAGGGTAGCCAGATGTACAGTGGCGCGTCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCGTTGccgcaggc          >  1:3655137/1‑143 (MQ=14)

                                                                                    tgCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCGTTGccgcaggcg         <  1:213507/150‑9 (MQ=17)

                                                                                     gCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCGTTGccgcaggcga        >  1:2862995/1‑141 (MQ=17)

                                                                                     gCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCGTTGccgcaggcga        >  1:78266/1‑141 (MQ=17)

                                                                                      cGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCGTTGccgcaggcgac       >  1:4150449/1‑140 (MQ=14)

                                                                                      cGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCGTTGccgcaggcgac       >  1:1256851/1‑140 (MQ=14)

                                                                                       gTAAAGTCTGGCGTCAGTTGTTACGGGAAGGTATCAGAGTGGCCAGATGCACTGTGGCACGTCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAGGGTCCGTACGACCATCAGCCGGAAAGCCGTTGccgcaggcgacc      <  1:2941572/150‑12 (MQ=21)

                                                                                       gTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCGTTGccgcagg           >  1:3777522/1‑139 (MQ=17)

                                                                                       gTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGCAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGATTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCGTTGccgcaggcgacc      <  1:3791135/150‑12 (MQ=14)

                                                                                         aaaGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCGTTGccgcaggcgaccgc    <  1:715423/150‑14 (MQ=14)

                                                                                           aGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCGTTGccgcaggcgaccgcgt  <  1:1004256/150‑16 (MQ=11)

                                                                                           aGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCGTTGccgcaggcgaccgcgt  >  1:13437/1‑135 (MQ=11)

                                                                                           aGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCGTTGccgcaggcgaccgcgt  >  1:1430538/1‑135 (MQ=11)

                                                                                           aGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCGTTGccgcaggcgaccgcgt  >  1:1464843/1‑135 (MQ=11)

AACGCAGTGCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACAGCGCGTATACGATGAAAATCACAAGGTATACGGTGTGCGTAAAGTCTGGCGTCAGTTGTTACGGGAAGGTATCAGAGTGGCCAGATGCACTGTGGCACGTCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCGTTGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  Xen14_202/196‑421

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`