Predicted mutation
evidence seq id position mutation annotation gene description
RA Xen14_202 294 C→A intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Xen14_2022940CA92.5% 2239.8 / 138.2 887intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (33/33);  new base A (427/392);  total (460/427)
Fisher's exact test for biased strand distribution p-value = 7.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 888 total aligned reads displayed.

TTGCCCCGTCAACGTATTACCACTGTCAGCAACAGCGACATCATCCGGATAAACGCAGTGCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACAGCGCGTATACGATGAAAATCACAAGGTATACGGTGTGCGTAAAGTCTGGCGTCAGTTGTTACGGGAAGGTATCAGAGTGGCCAGATGCACTGTGGCACGTCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCGTTGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  Xen14_202/145‑421
                                                                                                                                                     |                                                                                                                                              
  gCCCCGTCAACGTATTACCACTGTCAGCAACAGCGACATCATCCGGATAAACGCAGTGCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACAGCGCGTATACGATGAAAATCACAAGGTATACGGTGTGCGTAAAGTCTGGCGt                                                                                                                                              >  1:4050832/1‑150 (MQ=255)
     ccGTCAACGTATTACCACTGTCAGCAACAGCGACATCATCCTGATAAACGCAGTGCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAg                                                                                                                                           <  1:1258646/150‑1 (MQ=255)
     ccGTCAACGTATTACCACTGTCAGCAACAGCGACATCATCCTGATAAACGCAGTGCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAg                                                                                                                                           >  1:439640/1‑150 (MQ=255)
         cAACGTATTACCACTGTCAGCAACAGCGACATCATCCTGATAAACGCAGCGCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAgttgt                                                                                                                                       >  1:2873452/1‑150 (MQ=255)
            cGTATTACCACTGTCAGCAACAGCGACATCATCCTGATAAACGCAGTGCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTAc                                                                                                                                    <  1:1587449/150‑1 (MQ=255)
             gTATTACCACTGTCAGCAACAGCGACATCATCCTGATAAACGCAGTGCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACg                                                                                                                                   >  1:630131/1‑150 (MQ=255)
               aTTACCACTGTCAGCAACAGCGACATCATCCTGATAAACGCAGTGCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACggg                                                                                                                                 >  1:2154203/1‑150 (MQ=255)
                  aCCACTGTCAGCAACAGCGACATCATCCTGATAAACGCAGTGCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAg                                                                                                                              <  1:2373770/150‑1 (MQ=255)
                  aCCACTGTCAGCAACAGCGACATCATCCTGATAAACGCAGTGCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAg                                                                                                                              >  1:3854375/1‑150 (MQ=255)
                   ccACTGTCAGCAACAGCGACATCATCCTGATAAACGCAGTGCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAgg                                                                                                                             >  1:854420/1‑150 (MQ=255)
                   ccACTGTCAGCAACAGCGACATCATCCTGATAAACGCAGTGCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAgg                                                                                                                             >  1:2261931/1‑150 (MQ=255)
                   ccACTGTCAGCAACAGCGACATCATCCTGATAAACGCAGTGCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAgg                                                                                                                             >  1:2709532/1‑150 (MQ=255)
                   ccACTGTCAGCAACAGCGACATCATCCGGATAAACGCAGTGCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACAGCGCGTATACGATGAAAATCACAAGGTATACGGTGTGCGTAGAGTCTGGCGTCAGTTGTTACGGGAAgg                                                                                                                             <  1:2615501/150‑1 (MQ=255)
                      cTGTCAGCAACAGCGACATCATCCTGATAAACGCAGTGCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAggaat                                                                                                                          <  1:259296/150‑4 (MQ=255)
                      cTGTCAGCAACAGCGACATCATCCTGATAAACGCAGTGCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAggaat                                                                                                                          <  1:2442572/150‑4 (MQ=255)
                       taactgtctcaaaagtgaccataagctctcctgaatcaggagagcagattaccccctggatctgatttcaggcgttgggtgtggatcactattgcaccgttcgtgacaGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATc                                                                                                                         >  1:2551249‑M2/109‑150 (MQ=255)
                        gTCAGCAACAGCGACATCATCCTGATAAACGCAGTGCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCa                                                                                                                        >  1:481384/1‑150 (MQ=255)
                        gTCAGCAACAGCGACATCATCCTGATAAACGCAGTGCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCa                                                                                                                        >  1:3177384/1‑150 (MQ=255)
                          ctgtctcaaaagtgaccataagctctcctgaatcaggagagcagattaccccctggatctgatttcaggcgttgggtgtggatcactattgcaccgttcgtgacaGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATcagg                                                                                                                      <  1:2390151‑M2/45‑2 (MQ=255)
                          cAGCAACAGCGACATCATCCTGATAAACGCAGTGCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCagg                                                                                                                      >  1:769063/1‑149 (MQ=255)
                               aCAGCGACATCATCCTGATAAACGCAGTGCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCagggtagc                                                                                                                 <  1:3019318/150‑7 (MQ=255)
                                 aGCGACATCATCCTGATAAACGCAGTGCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCa                                                                                                               >  1:1409926/1‑150 (MQ=255)
                                  gCGACATCATCCTGATAAACGCAGTGCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACTGTGCGTATACGATGGGAATCATCAGGTATACTGTGTGCGTAAAGTCTGGAGGCAGTTGTTACGGGAAGGAATCAGGGTAGCCAg                                                                                                              <  1:2751574/150‑1 (MQ=255)
                                  gCGACATCATCCTGATAAACGCAGTGCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAg                                                                                                              >  1:2910200/1‑150 (MQ=255)
                                  gCGACATCATCCTGATAAACGCAGTGCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAg                                                                                                              >  1:2846778/1‑150 (MQ=255)
                                   cGACATCATCCTGATAAACGCGGTGCCCGTGCGCAGCGTGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGa                                                                                                             <  1:2404078/150‑1 (MQ=255)
                                    gACATCATCCTGATAAACGCAGTGCCCGTGCGCAGCGCGATGACGGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGAt                                                                                                            >  1:3701043/1‑150 (MQ=255)
                                     aCATCATCCTGATAAACGCAGTGCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATTGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATg                                                                                                           >  1:1734415/1‑150 (MQ=255)
                                      catcatCCTGATAAACGCAGTGCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGA‑TCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTGCGGGAAGGAATCAGGGTAGCCAGATgta                                                                                                         >  1:2974491/1‑148 (MQ=255)
                                         catcCTGATAAACGCAGTGCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATgtaca                                                                                                       >  1:2027082/1‑146 (MQ=255)
                                              tgATAAACGCAGTGCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGc                                                                                                  >  1:1684111/2‑150 (MQ=255)
                                              tgATAAACGCAGTGCCCGTGCGCAACGCGATGACTGGCTGAAGGAAGAGACACTGCGCGTATACGACGGGAATCATCAGATCTACGGTGTGCGTAACGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGc                                                                                                  >  1:2002997/2‑150 (MQ=255)
                                               gATAAACGCAGTGCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGcg                                                                                                 >  1:3280876/1‑149 (MQ=255)
                                                ctctcctgaatcaggagagcagattaccccctggatctgatttcaggcgttgggtgtggatcactattgcaccgttcgtgacaGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTggcgc                                                                                                >  1:4122764‑M2/84‑148 (MQ=255)
                                                aTAAACGCAGTGCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGcgc                                                                                                >  1:1224303/1‑148 (MQ=255)
                                                aTAAACGCAGTGCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGcgc                                                                                                >  1:267904/1‑148 (MQ=255)
                                                 tctcctgaatcaggagagcagattaccccctggatctgatttcaggcgttgggtgtggatcactattgcaccgttcgtgacaGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGtggcgcg                                                                                               <  1:2243589‑M2/68‑4 (MQ=255)
                                                 tAAACGCAGTGCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGNGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGcgcg                                                                                               >  1:3752060/1‑147 (MQ=255)
                                                  aaaCGCAGTGCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTCGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGcgcgc                                                                                              >  1:1915181/1‑146 (MQ=255)
                                                   aaCGCAGTGCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGcgtgcc                                                                                             >  1:2565152/1‑145 (MQ=255)
                                                   aaCGCAGTGCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGcgcgcc                                                                                             <  1:2939048/150‑6 (MQ=255)
                                                   aaCGCAGTGCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGATATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGcgcgcc                                                                                             >  1:3244306/1‑145 (MQ=255)
                                                     cGCAGTGCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGcgcgcctc                                                                                           <  1:1619633/150‑8 (MQ=255)
                                                       cAGTGCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCAt                                                                                         <  1:3783660/150‑1 (MQ=255)
                                                           gCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCAtcgca                                                                                     >  1:1715742/1‑146 (MQ=255)
                                                            cccGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCTGGGTAGCCAGATGTACAGTGGCCCGCCTCATGGCgg                                                                                    >  1:1141866/1‑150 (MQ=255)
                                                                  cgcAGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATgg                                                                              <  1:1039936/150‑1 (MQ=255)
                                                                    cAGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGGACAGTGGCGCGCCTCATGGCGGTTATGGGa                                                                            <  1:3124657/150‑1 (MQ=255)
                                                                       cgcgATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACtt                                                                         <  1:3807914/150‑1 (MQ=255)
                                                                        gcgATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTg                                                                        >  1:725640/1‑150 (MQ=255)
                                                                         cgATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGc                                                                       >  1:694670/1‑150 (MQ=255)
                                                                         cgATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGc                                                                       <  1:3486944/150‑1 (MQ=255)
                                                                            tGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCgg                                                                    <  1:1788351/150‑1 (MQ=255)
                                                                            tGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCgg                                                                    <  1:1174533/150‑1 (MQ=255)
                                                                              aCTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGNCGCGCCTCATGGCGGTTATGGGACTTGCCGgtg                                                                  >  1:1516775/1‑150 (MQ=255)
                                                                               tggatctgatttcaggcgttgggtgtggatcacgattgcaccgttcgtgacaGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGgtgt                                                                 <  1:3531050‑M2/98‑1 (MQ=255)
                                                                               cTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCgg                                                                                    <  1:2329818/131‑1 (MQ=255)
                                                                                tGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGtt                                                                <  1:506327/150‑1 (MQ=255)
                                                                                  atctgatttcaggcgttgggtgtggatcgctattgcaccgttcgtgacaGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTtct                                                              >  1:3353426‑M2/50‑150 (MQ=255)
                                                                                   cTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGGGGTTATGGGACTTGCCGGTGTtctc                                                             >  1:3950426/1‑150 (MQ=255)
                                                                                   cTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTtctc                                                             >  1:1314409/1‑150 (MQ=255)
                                                                                    tGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTcc                                                            >  1:4095328/1‑150 (MQ=255)
                                                                                        tttcaggcgttgggtgtggatcactattgcaccgttcgtgacaGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCgggg                                                        >  1:596474‑M2/44‑150 (MQ=255)
                                                                                        gaaagaGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCTgggg                                                        <  1:2806111/150‑1 (MQ=255)
                                                                                        gaaagaGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCgggg                                                        >  1:4236692/1‑150 (MQ=25)
                                                                                        gaaagaGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCgggg                                                        <  1:2301658/150‑1 (MQ=25)
                                                                                               aTACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAg                                                 <  1:136641/150‑1 (MQ=21)
                                                                                                   gggtgtggatcactattgcaccgttcgtgacaGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTcc                                             <  1:881097‑M2/118‑1 (MQ=255)
                                                                                                    gcgcGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCCCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCg                                            <  1:3899893/150‑1 (MQ=21)
                                                                                                    gcgcGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGGCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCg                                            <  1:4184302/150‑1 (MQ=21)
                                                                                                       gtggatcactattgcaccgttcgtgacaGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTAc                                         >  1:2102093‑M2/29‑150 (MQ=255)
                                                                                                       cgTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTAc                                         >  1:906012/1‑150 (MQ=21)
                                                                                                         tataCGATGGGAATCATCAGGTAGACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACGGTGGCGCGCCTCATGGCGTTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGa                                       <  1:3582578/150‑1 (MQ=18)
                                                                                                         ggatcactattgcaccgttcgtgacaGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGa                                       >  1:3509608‑M2/27‑150 (MQ=255)
                                                                                                             cgatgggaatcatcaGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCAt                                   >  1:2496435/15‑150 (MQ=18)
                                                                                                               atgggaatcatcaGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCa                                 >  1:8098/13‑150 (MQ=18)
                                                                                                               atgggaatcatcaGGTATACGGTGTGCGTAAAGTCTGGAGTCAGGTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCa                                 <  1:3315646/138‑1 (MQ=18)
                                                                                                                 gggaatcatcaGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGc                               >  1:3207631/11‑150 (MQ=18)
                                                                                                                   gaatcatcaGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCTGGGTAAAAAGGTCCGTACGACCATCAGCCg                             >  1:1870767/9‑150 (MQ=18)
                                                                                                                        atcaagTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAg                        <  1:108805/145‑1 (MQ=18)
                                                                                                                        atcaGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAGTCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGATTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAg                        >  1:586638/4‑150 (MQ=18)
                                                                                                                        atcaGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAg                        <  1:197311/147‑1 (MQ=18)
                                                                                                                         tcaGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGc                       >  1:2207612/3‑150 (MQ=18)
                                                                                                                         tcaGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGc                       <  1:3664634/148‑1 (MQ=18)
                                                                                                                           aGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCg                     <  1:401426/150‑1 (MQ=18)
                                                                                                                            ggTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCAGGAAAGCCGt                    >  1:3623410/1‑150 (MQ=18)
                                                                                                                               gacaGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCGTTGc                 <  1:563799‑M2/146‑1 (MQ=255)
                                                                                                                               ataCGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCGTTGc                 <  1:3040851/150‑1 (MQ=18)
                                                                                                                                   ggTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCGTTGccgca             <  1:3400825/150‑5 (MQ=17)
                                                                                                                                     tgtgCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCGTTGccgcagg           <  1:526127/150‑7 (MQ=17)
                                                                                                                                      gtgCGTAAAGTCTGGGGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCGTTGccgcaggc          <  1:1538788/150‑8 (MQ=17)
                                                                                                                                       tgCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCGTTGccgcaggcg         <  1:3149180/150‑9 (MQ=17)
                                                                                                                                        gCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCGTTGccgcaggcga        >  1:1452296/1‑141 (MQ=17)
                                                                                                                                         cGTAACGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCCTAGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCGTTGccgcaggcgac       >  1:3454315/1‑140 (MQ=14)
                                                                                                                                         cGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCGTTGccgcaggcgac       >  1:4150449/1‑140 (MQ=14)
                                                                                                                                             aaGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCGTTGccgcaggcgaccgcg   <  1:183362/150‑15 (MQ=14)
                                                                                                                                              aGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCGTTGccgcaggcgaccgcgt  >  1:13437/1‑135 (MQ=11)
                                                                                                                                              aGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCGTTGccgcaggcgaccgcgt  <  1:1004256/150‑16 (MQ=11)
                                                                                                                                              aGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCGTTGccgcaggcgaccgcgt  >  1:1464843/1‑135 (MQ=11)
                                                                                                                                              aGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGGAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCGTTGccgcaggcgaccgcgt  <  1:952268/150‑16 (MQ=11)
                                                                                                                                                     |                                                                                                                                              
TTGCCCCGTCAACGTATTACCACTGTCAGCAACAGCGACATCATCCGGATAAACGCAGTGCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACAGCGCGTATACGATGAAAATCACAAGGTATACGGTGTGCGTAAAGTCTGGCGTCAGTTGTTACGGGAAGGTATCAGAGTGGCCAGATGCACTGTGGCACGTCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCGTTGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  Xen14_202/145‑421

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑