BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	Xen14_191	251	A→G	intergenic (–/–)	– / –	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Xen14_191	251	0	A	G	88.6%	1670.4 / 139.4	802	intergenic (–/–)	–/–	–/–
Reads supporting (aligned to +/- strand): ref base A (48/43); new base G (333/378); total (381/421)
Fisher's exact test for biased strand distribution p-value = 3.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01

Only 100 of 803 total aligned reads displayed.

ATGCCAAACGTGTTTACCGGATCATGAACCGCCCCGGGAATCCTGGAGACTAAACTTCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAACGGGCAGTC‑CGTATGGTTCTGGAAAGTCAGAGCGAATATGACTCACAATGGGCGACAATTTGTTCCATTGCTCCAAAGATTGGCTGTACGCCGGAGACTCTGCGTGTCTGGGTTCGCCAGCATGAGCGGGATACCGGGGGCGGTGATGGAGGGCTCACCACCGCTGAACGTCAGCGTCTG  >  Xen14_191/104‑400

         tttccgtaactttactGAACCGCCCTGGGAATCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGAc                                                                                                                                            >  1:573764/16‑150 (MQ=17)

           gtTTACCGGATCATGAACCGCCCCGGGAATCCTGGAGACTAAACTTCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAACGGGCAGTC‑CGTATGGTTCTGGAAAGTCAGAGCGAATATGACTc                                                                                                                                          >  1:435448/1‑150 (MQ=255)

              tcaatctgcttGAACCGCCCCGGGAATCCTGGAGACTAAACTCCCTGAGAAAGAGCTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGAc                                                                                                                                            <  1:1772543/135‑1 (MQ=14)

                ccGGATCATGAACCGCCCCGGGAATCCTGGAGACTAAACTTCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAACGGGCAGTC‑CGTATGGTTCTGGAAAGTCAGAGCGAATATGACTCACAAt                                                                                                                                     >  1:1081654/1‑150 (MQ=255)

                  agttattGAACCGCCCCGGGAATCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGC‑GACTCGTATAGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTgg                                                                                                                                   >  1:2722517/7‑150 (MQ=14)

                   tgacctGAACCGCCCCGGGAATCCTGGAGACTCAACTCCCTAAGAAAGAGGTACACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTCTGGTTCTGTAAAGTCAGGGCGAATATGACTCACAGTggg                                                                                                                                  >  1:70516/6‑150 (MQ=17)

                   gttattGAACCGCCCCGGGAATCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTggg                                                                                                                                  >  1:1464317/6‑150 (MQ=17)

                      attgatccGCCCCGGGAATCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCgg                                                                                                                               <  1:1336716/144‑2 (MQ=18)

                       ttGAACCGCCCCGGGAATCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCggc                                                                                                                              <  1:4024377/149‑3 (MQ=18)

                        tGAACCGCCCCGGGAATCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCggca                                                                                                                             <  1:3918988/150‑4 (MQ=18)

                          aaCCGCCCCGGGAATCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAAt                                                                                                                           <  1:643084/150‑1 (MQ=18)

                           aCCGCCCCGGGAATCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAAtt                                                                                                                          >  1:1406417/1‑150 (MQ=18)

                            ccGCCCCGGGAATCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAAttt                                                                                                                         <  1:225430/150‑1 (MQ=18)

                             cGCCCCGGGAATCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAGCAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGACGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTg                                                                                                                        <  1:1222592/150‑1 (MQ=18)

                             cGCCCCGGGAATCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTg                                                                                                                        >  1:1363743/1‑150 (MQ=18)

                             cGCCCCGGGAATCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTg                                                                                                                        >  1:173966/1‑150 (MQ=18)

                                cccGGGAATCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTc                                                                                                                     <  1:3106116/150‑1 (MQ=18)

                                cccGGGAATCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTc                                                                                                                     >  1:368535/1‑150 (MQ=18)

                                cccGGGAATCCTGGAGACTAAACCCCCTGAGAAAGAGGTAGACAGGATGACTAGAAATACTCTTTTGTCCCTCGAAGTCCGTCAGAGGGCGATT‑TGTATGGTTCTGGAAAGGCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTc                                                                                                                     <  1:3411440/150‑1 (MQ=12)

                                   gggAATCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCAt                                                                                                                  <  1:3591930/150‑1 (MQ=21)

                                     gAATCCTGGAGACTAAACTTCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAACGGGCAGTC‑CGTATGGTTCTGGAAAGTCAGAGCGAATATGACTCACAATGGGCGACAATTTGTTTCATTg                                                                                                                >  1:592052/1‑150 (MQ=255)

                                        tCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATG‑CGTATGGTCCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGGTCCATTGccc                                                                                                             <  1:3202737/150‑3 (MQ=18)

                                            tgagaCTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCaaa                                                                                                         <  1:1291993/149‑1 (MQ=21)

                                            ggAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCTATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTg                                                                                                                        >  1:2309973/1‑135 (MQ=21)

                                            ggAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCaaa                                                                                                         >  1:1052283/1‑150 (MQ=21)

                                             gagaCTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCTCCAAAg                                                                                                        <  1:4095319/150‑1 (MQ=25)

                                             gagaCTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAATGTGCGGCAATTTGTTCCATTGCCCCAAAg                                                                                                        <  1:3056774/150‑1 (MQ=21)

                                              agaCTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGa                                                                                                       <  1:1543803/150‑1 (MQ=21)

                                                aCTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGAtt                                                                                                     <  1:475847/150‑1 (MQ=25)

                                                  tAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTgg                                                                                                   <  1:1162536/150‑1 (MQ=25)

                                                   aaaCTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGc                                                                                                  <  1:3739840/150‑1 (MQ=25)

                                                    aaCTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCt                                                                                                 <  1:2791383/150‑1 (MQ=25)

                                                     actcccTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTg                                                                                                <  1:3846634/146‑1 (MQ=25)

                                                      ctcccTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGt                                                                                               >  1:1882817/4‑150 (MQ=25)

                                                      ctcccTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGt                                                                                               <  1:1422321/147‑1 (MQ=25)

                                                        cccTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTAc                                                                                             >  1:2149999/2‑150 (MQ=25)

                                                             agaAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCgg                                                                                        >  1:2118426/1‑150 (MQ=37)

                                                              gaAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGga                                                                                       <  1:2674511/150‑1 (MQ=37)

                                                              gaAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGAGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGga                                                                                       >  1:23662/1‑150 (MQ=25)

                                                               aaaGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGgag                                                                                      <  1:1005238/150‑1 (MQ=37)

                                                               aaaGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCAGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGgag                                                                                      <  1:3313120/150‑1 (MQ=25)

                                                                aaGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGgaga                                                                                     >  1:1817462/1‑150 (MQ=37)

                                                                aaGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGATTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGgaga                                                                                     >  1:3526856/1‑150 (MQ=37)

                                                                  gagGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGAct                                                                                   >  1:1782685/1‑150 (MQ=255)

                                                                    ggTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCAGAGActct                                                                                 >  1:2474796/1‑150 (MQ=255)

                                                                    ggTAAACAGGATGACTAAAAATAATCGTTTTTTCCCCGAAGTCAGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGATTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGActct                                                                                 >  1:4221144/1‑150 (MQ=255)

                                                                         aCAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCgtg                                                                            <  1:3787085/150‑1 (MQ=255)

                                                                            ggATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAACGGGCAGTC‑CGTATGGTTCTGGAAAGTCAGAGCGAATATGACTCACAATGGGCGACAATTTGTTCCATTGCTCCAAAGATTGGCTGTACGCCGGAGACTCTGCGTGTCt                                                                         >  1:2013921/1‑150 (MQ=255)

                                                                             gaggACTAAAAATACTCCTTTTTCCCCCGAATTCTGTCAACGGGCAGTC‑CTTATTGTTCTGGAAAGTCAGAGCGAATATGACTCACAATGGGCGACAATTTTTTCCATTGCTCCAAAGAGTTGCGGTCCGCCGGAGACTCTGCGTGTCTg                                                                        <  1:4178082/147‑1 (MQ=255)

                                                                             gATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTg                                                                        >  1:2893102/1‑150 (MQ=255)

                                                                             gATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTg                                                                        >  1:1259777/1‑150 (MQ=255)

                                                                                 aCTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGta                                                                    <  1:2542788/150‑2 (MQ=255)

                                                                                  cTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCGATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGtac                                                                   >  1:2556145/1‑148 (MQ=255)

                                                                                  cTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGtac                                                                   >  1:157268/1‑148 (MQ=255)

                                                                                    aaaaaTACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGCTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTACCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGc                                                                 >  1:4261168/1‑150 (MQ=255)

                                                                                     aaaaTACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTGCGcc                                                                >  1:754331/1‑150 (MQ=255)

                                                                                      aaaTACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCa                                                               <  1:1801970/150‑1 (MQ=255)

                                                                                         tACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCa                                                            >  1:1621734/1‑150 (MQ=255)

                                                                                         tACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCa                                                            <  1:828668/150‑1 (MQ=255)

                                                                                          aCTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATTACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCAt                                                           <  1:2446819/150‑1 (MQ=255)

                                                                                          aCTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGGCTCTGCGTGTCTGGGTACGCCAGCAt                                                           <  1:3244930/150‑1 (MQ=255)

                                                                                           cTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATg                                                          >  1:2387864/1‑150 (MQ=255)

                                                                                           cTCGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGATTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATg                                                          >  1:2200488/1‑150 (MQ=255)

                                                                                             cGTTTTTCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGGGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAg                                                        <  1:3556566/150‑1 (MQ=255)

                                                                                                ttttCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCgg                                                     >  1:2082948/1‑150 (MQ=255)

                                                                                                ttttCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCg                                                      <  1:3486261/149‑1 (MQ=255)

                                                                                                 tttCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAGGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCggg                                                    <  1:852636/150‑1 (MQ=255)

                                                                                                 tttCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCggg                                                    <  1:2849084/150‑1 (MQ=255)

                                                                                                   tCCCCCGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCGGGAt                                                  <  1:1500029/150‑1 (MQ=255)

                                                                                                    cccccGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCGGGATa                                                 >  1:222556/1‑150 (MQ=255)

                                                                                                    cccccGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCGGGATa                                                 >  1:2598079/1‑150 (MQ=255)

                                                                                                    cccccGAAGTCCGTCAGAGGGCGATT‑CGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCGGGATa                                                 >  1:79325/1‑150 (MQ=255)

                                                                                                    cccccGAAGTCCGTCAACGGGCAGTC‑CGTATGGTTCTGGAAAGTCAGAGCGAATATGACTCACAATGGGCGACAATTTGTTCCATTGCTCCAAAGATTGGCTGTACGCCGGAGACTCTGCGTGTCTGGGTTCGCCAGCATGAGCg                                                      <  1:4129319/145‑1 (MQ=255)

                                                                                                      cccGAAGTCCGTCAGAGGGCGATT‑CGTACGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCGGGATAcc                                               >  1:2915477/1‑150 (MQ=255)

                                                                                                               cGTCAACGGGCAGTC‑CGTATGGTTCTGGAAAGTCAGAGCGAATATGACTCACAATGGGCGACAATTTGTTCCATTGCTCCAAAGATTGGCTGTACGCCGGAGACTCTGCGTGTCTGGGTTCGCCAGCATGAGCGGGATACCGGGGGCGGt                                      >  1:1115999/1‑150 (MQ=255)

                                                                                                                cgtcagagggcgattcGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCGGGATACCGGAGGCGGt                                      <  1:2814240/135‑1 (MQ=255)

                                                                                                                 gtcagagggcgattcGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTCCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCGGGATACCGGAGGCGGTg                                     >  1:913698/15‑150 (MQ=255)

                                                                                                                  tcagagggcgattcGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCGGGATACCGGAGGCGGTgg                                    <  1:1978629/137‑2 (MQ=255)

                                                                                                                   cagagggcgattcGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCGGGATACCGGAGGCGGTGAt                                   <  1:3326761/138‑1 (MQ=255)

                                                                                                                   cagagggcgattcGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGAATCTGCGTGTCTGGGTACGCCAGCATGAGCGGGATACCGGAGGCGGTGAt                                   >  1:3274823/13‑150 (MQ=255)

                                                                                                                       gggcgattcGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGt                                                                     >  1:730198/9‑112 (MQ=255)

                                                                                                                         gcgattcGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCGGGATACCGGAGGCGGTGATGGCggg                             >  1:3364690/7‑150 (MQ=255)

                                                                                                                         cAGTC‑CGTATGGTTCTGGAAAGTCAGAGCGAATATGACTCACAATGGGCGACAATTTGTTCCATTGCTCCAAAGATTGGCTGTACGCCGGAGACTCTGCGTGTCTGGGTTCACCAGCATGAGCGGGATACCGGGGGCGGTGATGGAGGGc                            <  1:2346962/150‑1 (MQ=255)

                                                                                                                          cgattcGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCGGGATACCGGAGGCGGTGATGGCGGGc                            <  1:3965209/145‑1 (MQ=255)

                                                                                                                          cgattcGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCGGGATACCGGAGGCGGTGATGGCGGGc                            <  1:1675526/145‑1 (MQ=255)

                                                                                                                          cgattcGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCGGGATACCGGAGGCGGTGATGGCGGGc                            <  1:1833609/145‑1 (MQ=255)

                                                                                                                               cGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCGGGATACCGGAGGCGGTGATGGCGGGCTcacc                       <  1:4051160/150‑1 (MQ=255)

                                                                                                                               cGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCGGGATACCGGAGGAGGTGATGGCGGGCTcacc                       >  1:2577125/1‑150 (MQ=255)

                                                                                                                                gTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCGGGATACCGGAGGCGGTGATGGCGGGCTcacca                      >  1:66323/1‑150 (MQ=255)

                                                                                                                                 tATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCGGGATACCGGAGGCGGTGATGGCGGGCTcaccac                     <  1:530893/150‑1 (MQ=255)

                                                                                                                                 tATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCGGGATACCGGAGGCGGTGATGGCGGGCTcaccac                     >  1:1602020/1‑150 (MQ=255)

                                                                                                                                   tGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCGGGATACCGGAGGCGGTGATGGCGGGCTCACCACCg                   <  1:3652416/150‑1 (MQ=255)

                                                                                                                                    ggTTCTGGAAAGTCGGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCGGGATACCGGAGGCGGTGATGGCGGGCTCACCACCGc                  >  1:921409/1‑150 (MQ=255)

                                                                                                                                       tCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCGGGATACCGGAGGCGGTGATGGCGGGCTCACCACCGCTGa               >  1:3465288/1‑150 (MQ=255)

                                                                                                                                        cTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCGGGATACCGGAGGCGGTGATGGCGGGCTCACCACCGCTGaa              >  1:2954984/1‑150 (MQ=255)

                                                                                                                                        cTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCGGGATACCGGAGGCGGTGATGGCGGGCTCACCACCGCTGaa              <  1:1310306/150‑1 (MQ=255)

                                                                                                                                        cTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATCGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCGGGATACCGGAGGCGGTGATGGCGGGTTCACCACCGCTGaa              <  1:2997319/150‑1 (MQ=255)

                                                                                                                                         tGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCGGGATACCGGAGGCGGTGATGGCGGGCTCACCACCGCTGAAc             <  1:3618062/150‑1 (MQ=255)

                                                                                                                                          ggAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCGGGATACCGGGGGCGGTGATGGCGGGCTCACCACCGCTGAACg            >  1:2631917/1‑150 (MQ=255)

                                                                                                                                            aaaGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCGGGATACCGGAGGCGGTGATGGCGGGCTCACCACCGCTGAACGTc          >  1:3026241/1‑150 (MQ=255)

ATGCCAAACGTGTTTACCGGATCATGAACCGCCCCGGGAATCCTGGAGACTAAACTTCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAACGGGCAGTC‑CGTATGGTTCTGGAAAGTCAGAGCGAATATGACTCACAATGGGCGACAATTTGTTCCATTGCTCCAAAGATTGGCTGTACGCCGGAGACTCTGCGTGTCTGGGTTCGCCAGCATGAGCGGGATACCGGGGGCGGTGATGGAGGGCTCACCACCGCTGAACGTCAGCGTCTG  >  Xen14_191/104‑400

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`