BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	Xen14_143	967	C→T	intergenic (‑661/–)	Xen14_04759 ← / –	hypothetical protein/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Xen14_143	967	0	C	T	88.0%	2932.1 / 252.1	1325	intergenic (‑661/–)	Xen14_04759/–	hypothetical protein/–
Reads supporting (aligned to +/- strand): ref base C (87/72); new base T (599/567); total (686/639)
Fisher's exact test for biased strand distribution p-value = 4.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.97e-01

Only 100 of 1333 total aligned reads displayed.

GTCCGGGATGATCGCAATGCGGGTTCGTCATCTCCTCCGGCGGTCTGGTTCGCGTACTCACCGAACCGGCAGGGGAAACACCCGGAGCAACACCTCCGCCCCTTCC‑GGGGTATCCTGCAGGCGGATGCGTTCACAGGTTATGACAGGCTGTTCAGTGCAGAACGTGAAGGTGGTGCGCTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGAGAAAAATCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGCCCTGAAGCGAATCAGTGAACTGTAC‑  >  Xen14_143/820‑1107

 tCCGGGATGATCGCAATGTGGGTTCGTCATCTCCTCCAGCGGTCTGGTTCGCGTACTCGCCGAACCGGCAGGGGAAACACCCGGAGCAACACCTTCGCCCCTTCC‑GGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGt                                                                                                                                            >  1:3953773/1‑150 (MQ=255)

 tCCGGGATGATCGCAATGTGGGTTCGTCATCTCCTCCAGCGGTCTGGTTCGCGTACTCGCCGAACCGGCAGGGGAAACACCCGGAGCAACACCTTCGCCCCTTCC‑GGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGt                                                                                                                                            <  1:1448797/150‑1 (MQ=255)

      gatgatCGCAATGTGGGTTCGTCATCTCCTCCAGCGGTCTGGTTCGCGTACTCGCCGAACCGGCAGGGGAAACACCCGGAGCAACACCTTCGCCCCTTCC‑GGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGt                                                                                                                                       <  1:1972937/150‑1 (MQ=255)

        tgatCGCAATGTGGGTTCGTCATCTCCTCCAGCGGTCTGGTTCGCGTACTCGCCGAACCGGCAGGGGAAACACCCGGAGCAACACCTTCGCCCCTTCC‑GGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGc                                                                                                                                     <  1:3045869/150‑1 (MQ=255)

        tgatCGCAATGTGGGTTCGTCATCTCCTCCAGCGGTCTGGTTCGCGTACTCGCCGAACCGGCAGGGGAAACACCCGGAGCAACACCTTCGCCCCTTCC‑GGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGc                                                                                                                                     <  1:1596012/150‑1 (MQ=255)

        tgatCGCAATGTGGGTTCGTCATCTCCTCCAGCGGTCTGGTTCGCGTACTCGCCGAACCGGCAGGGGAAACACCCGGAGCAACACCTTCGCCCCTTCC‑GGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGc                                                                                                                                     >  1:1519771/1‑150 (MQ=255)

        tgatCGCAATGTGGGTTCGTCATCTCCTCCAGCGGTCTGGTTCGCGTACTCGCCGAACCGGCAGGGGAAACACCCGGAGCAACACCTTCGCCCCTTCC‑GGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGc                                                                                                                                     <  1:1493012/150‑1 (MQ=255)

        tgatCGCAATGTGGGTTCGTCATCTCCTCCAGCGGTCTGGTTCGCGTACTCGCCGAACCGGCAGGGGAAACACCCGGAGCAACACCTTCGCCCCTTCC‑GGGGTATCCTGCAGGCGGATGCGTTCACAGGTCACGACAGGTTGTTCAGTGc                                                                                                                                     >  1:842871/1‑150 (MQ=255)

        tgatCGCAATGCGGGTTCGTCGTCTCCTCCGGCGGTCTGGTTCGCGTACTCACCGAACCGGCAGGGGAAACACCCGGAGCAACACCTCCGCCCCTTCC‑GGGGTATCCCGCAGGCGGATGCGTTCACAGGTTATGACAGGCTGTTCAGTGc                                                                                                                                     <  1:3863831/150‑1 (MQ=255)

        tgatCGCAATGCGGGTTCGTCATCTCCTCCGGCGGTCTGGTTCGCGTACTCACCGAACCGGCAGGGGAAACACCCGGAGCAACACCTCCGCCCCTTCC‑GGGGTATCCTGCAGGCGGATGCGTTCACAGGTTATGACAGGCTGTTCAGTGc                                                                                                                                     >  1:329169/1‑150 (MQ=255)

          atCGCAATGTGGGTTCGTCATCTCCTCCAGCGGTCTGGTTCGCGTACTCGCCGAACCGGCAGGGGAAACACCCGGAGCAACACCTTCGCCCCTTCC‑GGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAg                                                                                                                                   >  1:2553807/1‑150 (MQ=255)

          atCGCAATGTGGGTTCGTCATCTCCTCCAGCGGTCTGGTTCGCGTACTCGCCGAACCGGCAGGGGAAACACCCGGAGCAACACCTTCGCCCCTTCC‑GGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAg                                                                                                                                   <  1:547622/150‑1 (MQ=255)

           tCGCAATGTGGGTTCGTCATCTCCTCCAGCGGTCTGGTTCGCGTACTCGCCGAACCGGCAGGGGAAACACCCGGAGCAACACCTTCGCCCCTTCC‑TGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGa                                                                                                                                  <  1:2921994/150‑1 (MQ=255)

             gCAATGTGGGTTCGTCATCTCCTCCAGCGGTCTGGTTCGCGTACTCGCCGAACCGGCAGGGGAAACACCCGGAGCAACACCTTCGCCCCTTCC‑TGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAAc                                                                                                                                <  1:2328442/150‑1 (MQ=255)

               aaTGTGGGTTCGTCATCTCCTCCAGCGGTCTGGTTCGCGTACTCGCCGAACCGGCAGGGGAAACACCCGGAGCAACACCTTCGCCCCTTCC‑GGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGt                                                                                                                              >  1:229043/1‑150 (MQ=255)

                atgtgggTTCGTCATCTCCTCCAGCGGTCTGGTTCGCGTACTCGCCGAACCGGCAGGGGAAACACCCGGAGCAACACCTTCGCCCCTTCC‑GGGGTATCCTGCAGGCGGATGCGTTCACAGGTTATGACAGGTTGTTCAGTGCAGAACGTg                                                                                                                             >  1:3124835/5‑150 (MQ=255)

                     ggTTCGTCATCTCCTCCAGCGGTCTGGTTCGCGTACTCGCCGAACCGGCAGGGGAAACACCCGGAGCAACACCTTCGCCCCTTCC‑GGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAggt                                                                                                                        <  1:3426350/150‑1 (MQ=255)

                     ggTTCGTCATCTCCTCCAGCGGTCTGGTTCGCGTACTCGCCGAACCGGCAGGGGAAACACCCGGAGCAACACCTTCGCCCCTTCC‑GGGGTATCCTGCAGGCGGATGCGTTCAAAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAggt                                                                                                                        >  1:1309191/1‑150 (MQ=255)

                          gTCATCTCCTCCAGCGGTCTGGTTCGCGTACTCGCCGAACCGGCAGGGGAAACACCCGGAGCAACACCTTCGCCCCTTCC‑GGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTgc                                                                                                                   >  1:2417654/1‑150 (MQ=255)

                           tCATCTCCTCCGGCGGTCTGGTTCGCGTACTCACCGAACCGGCAGGGGAAACACCCGGAGCAACACCTCCGCCCCTTCC‑GGGGTATCCTGCAGGCGGATGCGTTCACAGGTTATGACAGGCTGTTCAGTGCAGAACGTGAAGGTGGTgcg                                                                                                                  <  1:2846958/150‑1 (MQ=255)

                                tcctccAGCGGTCTGGTTCGCGTACTCGCCGAACCGGCAGGGGAAACACCCGGAGCAACACCTTCGCCCCTTCC‑GGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGAc                                                                                                             <  1:2498161/150‑1 (MQ=255)

                                  ctccGGCGGTCTGGTTCGCGTACTCACCGAACCGGCAGGGGAAACACCCGGAGCAACACCTCCGCCCCTTCC‑GGGGTATCCTGCAGGCGGATGCGTTCACAGGTTATGACAGGCTGTTCAGTGCAGAACGTGAAGGTGGTGCGCTGACAg                                                                                                           >  1:516122/1‑150 (MQ=255)

                                  ctccAGCGGTCTGGTTCGCGTACTCGCCGAACCGGCAGGGGAAACACCCGGAGCNACACCTTCGCCCCTTCC‑GGGGTATCCTGCAGGCNGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGTAGAACGTGAAGGTGGTGCACTGACAg                                                                                                           >  1:3724077/1‑150 (MQ=255)

                                   tccagcggTCTGGTTCGCGTACTCGCCGAACCGGCAGGGGAAACACCCGGAGCAACACCTTCGCCCCTTCC‑GGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGa                                                                                                          <  1:3197134/146‑1 (MQ=255)

                                    ccagcggTCTGGTTCGCGTACTCGCCGAACCGGCAGGGGAAACACCCGGAGCAACACCTTCGCCCCTTCC‑GGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGaa                                                                                                         <  1:685461/147‑1 (MQ=255)

                                     cagtggTCTGGTTCGCGTACTCGCCGAACCGGCAGGGGAAACACCCGGAGCAACACCTCCGTCCCTTCC‑GGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAg                                                                                                        >  1:3388580/5‑150 (MQ=255)

                                     cagcggTCTGGTTCGCGTACTCGCCGAACCGGCAGGGGAAACACCCGGAGCAACACCTTCGCCCCTTCC‑GGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAg                                                                                                        >  1:3253798/3‑150 (MQ=255)

                                     cagcggTCTGGTTCGCGTACTCGCCGAACCGGCAGGGGAAACACCCGGAGCAACACCTCCGTCCCTTCC‑GGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAg                                                                                                        >  1:1074505/3‑150 (MQ=255)

                                      ggcggTCTGGTTCGCGTACTCACCGAACCGGCAGGGGAAACACCCGGAGCAACACCTCCGCCCCTTCC‑GGGGTATCCTGCAGGCGGATGCGTTCACAGGTTATGACAGGCTGTTCAGTGCAGAACGTGAAGGTGGTGCGCTGACAGAAGt                                                                                                       <  1:3012586/150‑1 (MQ=255)

                                       gcggTCTGGTTCGCGTACTCGCCGAACCGGCAGGGGAAATACCCGGAGCAACACCTCCGTCCCTTCC‑GGGGTATCCTGCAGGCGGATGCGTTCACAGGTTGCGACAGATTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGtt                                                                                                      <  1:1327880/150‑1 (MQ=255)

                                        cggTCTGGTTCGCGTACTCGCCGAACCGGCAGGGGAAACACCCGGAGCAACACCTTCGCCCCTTCC‑GGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTg                                                                                                     >  1:775706/1‑150 (MQ=255)

                                          gTCTGGTTCGCGTACTCGCCGAACCGGCAGGGGAAACACCCGGAGCAACACCTTCGCCCCTTCC‑GGGGTATCCTGCAGGCGGATGAGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCg                                                                                                   >  1:3808495/1‑150 (MQ=255)

                                           tCTGGTTCGCGTACTCGCCGAACCGGCAGGGGAAACACCCGGAGCAACACCTCCGTCCCTTCC‑GGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGt                                                                                                  <  1:2606156/150‑1 (MQ=255)

                                             tGGTTCGCGTACTCGCCGAACCGGCAGGGGAAACACCCGGAGCAACACCTCCGTCCCTTCC‑GGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGc                                                                                                >  1:3158701/1‑150 (MQ=255)

                                              ggTTCGCGTACTCGCCGAACCGGCAGGGGAAACACCCGGAGCAACACCTTCGCCCCTTCC‑GGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCt                                                                                               >  1:2258801/1‑150 (MQ=255)

                                              ggTTCGCGTACTCGCCGAACCGGCAGGGGAAACACCCGGAGCAACACCTTCGCCCCTTCC‑GGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCt                                                                                               <  1:1127663/150‑1 (MQ=255)

                                              ggTTCGCGTACTCGCCGAACCGGCAGGGGAAACACCCGGAGCAACACATTCGCCCCTTCC‑GGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCt                                                                                               >  1:1630597/1‑150 (MQ=255)

                                                ttCGCGTACTCGCCGAACCGGCAGGGGAAACACCCGGAGCAACACCTCCGTCCCTTCC‑GGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGCGCTgg                                                                                             >  1:1208844/1‑150 (MQ=255)

                                                  cgcgTACTCGCCGAACCGGCAGGGGAAACACCCGGAGCAACACCTCCGTCCCTTCC‑GGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGc                                                                                           >  1:2895575/1‑150 (MQ=255)

                                                   gcgTACTCGCCGAACCGGCAGGGGAAACACCCGGAGCAACACCTTCGCCCCTTCC‑GGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGcc                                                                                          <  1:3760926/150‑1 (MQ=255)

                                                   gcgTACTCGCCGAACCGGCAGGGGAAACACCCGGAGCAACACCTTCGCCCCTTCC‑GGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGcc                                                                                          <  1:2962891/150‑1 (MQ=255)

                                                        ctcgccGAACCGGCAGGGGAAACACCCGGAGCAACACCTTCGCCCCTTCC‑GGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGc                                                                                     >  1:1882342/5‑150 (MQ=255)

                                                        cTCACCGAACCGGCAGGGGAAACACCCGGAGCAACACCTCCGCCCCTTCC‑GGGGTATCCTGCAGGCGGATGCGTTCACAGGTTATGACAGGCTGTTCAGTGCAGAACGTGAAGGTGGTGCGCTGACAGAAGTTGCGTGCTGGGCCCATGc                                                                                     >  1:210647/1‑150 (MQ=255)

                                                          cgccGAACCGGCAGGGGAAACACCCGGAGCAACACCTCCGTCCCTTCC‑GGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGccc                                                                                   >  1:390765/3‑150 (MQ=255)

                                                          cACCGAACCGGCAGGGGAAACACCCGGAGCAACACCTCCGCCCCTTCC‑GGGGTATCCTGCAGGCGGATGCGTTCACAGGTTATGACAGGCTGTTCAGTGCAGAACGTGAAGGTGGTGCGCTGACAGAAGTTGCGTGCTGGGccc                                                                                         <  1:4128997/144‑1 (MQ=255)

                                                             cGAACCGGCAGGGGAAACACCCGGGGCAACACCTCCGCCCCTTCC‑GGGGTATCCTGCAGGCGGATGCGTTCACAGGTTATGACAGGCTGTTCAGTGCAGAACGTGAAGGTGGTGCGCTGACAGAAGTTGCGTGCTGGGCCCATGCCCGga                                                                                <  1:3590286/150‑1 (MQ=255)

                                                             cGAACCGGCAGGGGAAACACCCGGAGCAACACCTTCGCCCCTTCC‑GGGGTATCCTGCAGGCGGATGCGTTCATGGGTTACGACATGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACTGAAGTTGCGTGCTGGGCCCATGCCCggc                                                                                <  1:3628002/150‑2 (MQ=255)

                                                                 ccGGCAGGGGAAACACCCGGAGCAACACCTCCGTCCCTTCCAGGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGaa                                                                             >  1:626972/1‑150 (MQ=255)

                                                                  cGGCAGGGGAAACACCCGGAGCAACACCTTCGCCCCTTCC‑GGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGaaaa                                                                           <  1:2012160/150‑1 (MQ=255)

                                                                    gCAGGGGAAACACCCGGAGCAACACCTTCGCCCCTTCC‑GGGGTATCCTGCAGGCGGATGCATTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAAt                                                                         >  1:109194/1‑150 (MQ=255)

                                                                       ggggAAACACCCGGAGCAACACCTCCGCCCCTTCC‑GGGGTATCCTGCAGGCGGATGCGTTCACAGGTTATGACAGGCTGTTCAGTGCAGAACGTGAAGGTGGTGCGCTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGAGAAAAATCCa                                                                      >  1:221920/1‑150 (MQ=255)

                                                                       ggggAAACACCCAGAGCAATACCTCCGTCCCTTCC‑GGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAATGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATCCa                                                                      >  1:2667942/1‑150 (MQ=255)

                                                                        gggAAACACCCGGAGCAACACCTTCGCCTCTTCC‑GGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGATAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATCCAc                                                                     <  1:2054850/150‑1 (MQ=255)

                                                                        gggAAACACCCGGAGCAACACCTTCGCCCCTTCC‑GGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATCCAc                                                                     <  1:3696684/150‑1 (MQ=255)

                                                                        gggAAACACCCGGAGCAACACCTTCGCCCCTTCC‑GGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATCCAc                                                                     <  1:4187957/150‑1 (MQ=255)

                                                                        gggAAACACCCGGAGCAACACCTCCGTCCCTTCC‑GGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATCCAc                                                                     <  1:1783794/150‑1 (MQ=255)

                                                                        gggAAACACCCGGAGCAACACCTCCGTCCCTTCC‑GGGGTATCCTGCAGGCAGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATCCAc                                                                     >  1:3923749/1‑150 (MQ=255)

                                                                         ggAAACACCCGGAGCAACACCTTCGCCCCTTCC‑GGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACGGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATCCACg                                                                    <  1:3680976/150‑1 (MQ=255)

                                                                          gAAACACCCGGAGCAACACCTTCGCCCCTTCC‑GGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATCCACGa                                                                   >  1:2163646/1‑150 (MQ=255)

                                                                             acacCCGGAGCAACACCTTCGCCCCTTCC‑GGGGTATCCTGCAGGCGGATGCGTTCACAGGTTCCGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATCCACGATGt                                                                >  1:4083920/1‑150 (MQ=255)

                                                                               acCCGGAGCAACACCTTCGCCCCTTCC‑GGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAg                                                                                                        >  1:1834025/1‑108 (MQ=255)

                                                                                 ccGGAGCAACACCTCCGTCCCTTCC‑GGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATCCACGATGTATAc                                                            <  1:3503832/150‑1 (MQ=255)

                                                                                    gAGCAACACCTTCGCCCCTTCC‑GGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATCCACGATGTATACATc                                                         <  1:1573286/150‑1 (MQ=255)

                                                                                    gAGCAACACCTCCGCCCCTTCC‑GGGGTATCCTGCAGGCGGATGCGTTCACAGGTTATGACAGGCTGTTCAGTGCAGAACGTGAAGGTGGTGCGCTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGAGAAAAATCCACGATGTATACATc                                                         <  1:1710281/150‑1 (MQ=255)

                                                                                      gCAACACCTTCGCCCCTTCC‑GGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATCCACGATGTATACATcag                                                       >  1:1037170/1‑150 (MQ=255)

                                                                                      gCAACACCTTCGCCCCTTCC‑GGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATCCACGATGTATACATcag                                                       >  1:3087182/1‑150 (MQ=255)

                                                                                      gCAACACCTTCGCCCCTTCC‑GGGGTATCCTGCAGGCGGATACGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATCCACGATGTATACATcag                                                       <  1:2821555/150‑1 (MQ=255)

                                                                                      gCAACACCCTCGCCCCTTCC‑GGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATCCACGATGTATACATcag                                                       >  1:2079722/1‑150 (MQ=255)

                                                                                        aaCACCTCCGCCCCTTCC‑GGGGTATCCTGCAGGCGGATGCGTTCACAGGTTATGACAGGCTGTTCAGTGCAGAACGTGAAGGTGGTGCGCTGACAGAAGTTGCGTGCTGGGCCCATGCCCAGAGAAAAATCCACGATGTATACATcagca                                                     >  1:3357474/1‑150 (MQ=255)

                                                                                          cacCTTCGCCCCTTCC‑GGGGTATCTTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATCCACGATGTATACATCagcagc                                                   >  1:2357969/1‑150 (MQ=255)

                                                                                            ccttcGCCCCTTCA‑GGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATCCACGATGTATACATCAGCAGCaa                                                 >  1:1396539/5‑150 (MQ=255)

                                                                                              ttcGCCCCTTCC‑GGGGTATCCTGCAGGCGGAGGCGTTCACAGGTTATGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATCCACGATGTATACATCAGCAGCaaaa                                               <  1:886940/148‑1 (MQ=255)

                                                                                                     cTTCC‑GGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATCCACGATGTATACATCAGCAGCAAAAGTGCGAc                                        <  1:1168251/150‑1 (MQ=255)

                                                                                                     cTTCC‑GGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATCCACGATGTATACATCAGCAGCAAAAGTGCGAc                                        >  1:3999309/1‑150 (MQ=255)

                                                                                                        cc‑GGGGTATCCTGCCGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATCCACGATGTATACATCAGCAGCAAAAGTGCGACGGc                                     >  1:4238615/1‑150 (MQ=255)

                                                                                                         c‑GGGGTATCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCa                                    <  1:752306/150‑1 (MQ=255)

                                                                                                                 tCCTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGaagaag                             >  1:3543921/1‑150 (MQ=255)

                                                                                                                   cTGCAGGCGGATGCGTTCACAGGTTATGACAGGCTGTTCAGTGCAGAACGTGAAGGTGGTGCGCTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGAGAAAAATCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGcc                           <  1:1366877/150‑1 (MQ=255)

                                                                                                                   cTGCAGGCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCATTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATCCACGATGTATACATCGGCAGCAAAAGTGCGACGGCAGAAGAAGcc                           <  1:1745292/150‑1 (MQ=255)

                                                                                                                      cAGGCGGATGCGTTCACAGGTTATGACAGGCTGTTCAGTGCAGAACGTGAAGGTGGTGCGCTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGAGAAAAATCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGCCCTg                        >  1:2707789/1‑150 (MQ=255)

                                                                                                                        ggCGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGCCCTGaa                      <  1:459275/150‑1 (MQ=255)

                                                                                                                          cGGATGCGTTCACATGTTATGACAGGCTGTTCAGTGCAGAACGTGAAGGTGGTGCGCTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGAGAAAAATCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGCCCTGAAGc                    <  1:4215552/150‑1 (MQ=255)

                                                                                                                          cGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGCCCTGAAGc                    <  1:2635086/150‑1 (MQ=255)

                                                                                                                          cGGATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGCCCTGAAGc                    >  1:3319541/1‑150 (MQ=255)

                                                                                                                           ggATGCGTTCACAGGTTATGACAGGCTGTTCAGTGCAGAACGTGAAGGTGGTGCGCTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGAGAAAAATCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGCCCTGAAGCg                   >  1:4163241/1‑150 (MQ=255)

                                                                                                                           ggATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAGGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGCCCTGAAGCg                   <  1:942322/150‑1 (MQ=255)

                                                                                                                           ggATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGCCCTGAAGCg                   <  1:646608/150‑1 (MQ=255)

                                                                                                                            gATGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGCCCTGAAGCGa                  <  1:1953273/150‑1 (MQ=255)

                                                                                                                              tGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGCCCTGAAGCGAAt                <  1:1274728/150‑1 (MQ=255)

                                                                                                                              tGCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGGTGCGTGCTGGGCCCATGCCCGGCGAAAAATCCATGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGCCCTGAAGCGaa                 <  1:2757320/149‑1 (MQ=255)

                                                                                                                               gCGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGCCCTGAAGCGAATc               >  1:595560/1‑150 (MQ=255)

                                                                                                                                cGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGCCCTGAAGCGAATCa              >  1:2439544/1‑150 (MQ=255)

                                                                                                                                cGTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGCCCTGAAGCGAATCa              >  1:2573498/1‑150 (MQ=255)

                                                                                                                                 gTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGCCCTGAAGCGAATCAg             >  1:1266008/1‑150 (MQ=255)

                                                                                                                                 gTTCACAGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGCCCTGAAGCGAATCAg             >  1:3612583/1‑150 (MQ=255)

                                                                                                                                    cacaGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGCCCTGAAGCGAATCAGTGa          >  1:1806681/1‑150 (MQ=255)

                                                                                                                                    cacaGGTTACGACAGGTTGTTCAGTGCAGAACGTGAAGGTGATGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGCCCTGAAGCGAATCAGTGa          >  1:3465632/1‑150 (MQ=255)

                                                                                                                                           tATGACAGGCTGTTCAGTGCAGAACGTGAAGGTGGTGCGCTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGAGAAAAATCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGCCCTGAAGCGAATCAGTGAACTGTAc   >  1:729328/1‑150 (MQ=255)

                                                                                                                                            acgACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGCCCTGAAGCGAATCAGTGAACTGTAcg  <  1:4024421/148‑2 (MQ=255)

                                                                                                                                            acgACAGGTTGTTCAGTGCAGAACGTGAAGGTGGTGCACTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGCGAAAAATCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGCCCTGAAGCGAATCAGTGAACTGTAcg  <  1:1004016/148‑2 (MQ=255)

GTCCGGGATGATCGCAATGCGGGTTCGTCATCTCCTCCGGCGGTCTGGTTCGCGTACTCACCGAACCGGCAGGGGAAACACCCGGAGCAACACCTCCGCCCCTTCC‑GGGGTATCCTGCAGGCGGATGCGTTCACAGGTTATGACAGGCTGTTCAGTGCAGAACGTGAAGGTGGTGCGCTGACAGAAGTTGCGTGCTGGGCCCATGCCCGGAGAAAAATCCACGATGTATACATCAGCAGCAAAAGTGCGACGGCAGAAGAAGCCCTGAAGCGAATCAGTGAACTGTAC‑  >  Xen14_143/820‑1107

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`