BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	Xen14_143	594	C→T	intergenic (‑288/–)	Xen14_04759 ← / –	hypothetical protein/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Xen14_143	594	0	C	T	87.9%	2289.2 / 215.1	1150	intergenic (‑288/–)	Xen14_04759/–	hypothetical protein/–
Reads supporting (aligned to +/- strand): ref base C (65/74); new base T (484/527); total (549/601)
Fisher's exact test for biased strand distribution p-value = 8.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1158 total aligned reads displayed.

CTGAAAGTGATCCGCACAGAACGGGTAAAAAAAGCGTGTACAAAATGTGACTGCATCGTTGAAGCACCGGCGCCGTCCCGCCCGATAGAGCGTGGTATCGCGGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACACCTGCCACTGTATCGTCAGAGTGAAATCT‑TTGCCCGACAGGGTGTCGAACTGAGCCGTGCATTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACGCCGCTGAATGATGCCCTGTACCGTTATGTGATGAATACCCGCAAGCT  >  Xen14_143/445‑743

   aaaGTGATCCGCACAGAACGGGTAAAAAAAGCCTGTACAAAATGTGACTGTATTGTTGAAGCACCGGCGCCGTCCCGCCCGATAGAGCGTGGTATCGCGGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTg                                                                                                                                                     <  1:290227/150‑1 (MQ=11)

     aGTGATCCGCACGGAACGGGTAAAAAAAGCCTGTACAAAATGTGACTGTATTGTTGAAGCACCGGCGCCGTCCCGCCCGATAGAGCGTGGTATCGCGGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGcc                                                                                                                                                   <  1:834668/150‑1 (MQ=11)

         aTCCGCACAGAACGGGTAAAAAAAACCTGTACAAAATGTGACTGTATTGTTGAAGCACCGGCGCCGTCCCGCCCGATAGAGCGTGGTATCGCGGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTg                                                                                                                                               >  1:3723379/1‑150 (MQ=11)

          tCCGCACAGAATGGGTAAAAAAAGCCTGTACAAAATGTGACTGTATTGTTGAAGCACCGGCGCCGTCCCGCCCGATAGAGCGTGGTATCGCGGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGt                                                                                                                                              >  1:3634290/1‑150 (MQ=11)

            cGCACAGAACGGGTAAAAAAAGCCTGTACAAAATGTGACTGTATTGTTGAAGCACCGGCGCCGTCCCGCCCGATAGAGCGTGGTATCGCGGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTAt                                                                                                                                            >  1:656906/1‑150 (MQ=14)

                  gAACGGGTAAAAAAAGCCTGTACAAAATGTGACTGTATTGTTGAAGCACCGGCGCCGTCCCGCCCGATAGAGCGTGGTATCGCGGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCag                                                                                                                                      >  1:3164692/1‑150 (MQ=14)

                    aCGGGTAAAAAAAGCCTGTACAAAATGTGACTGTATTGTTGAAGCACCGGCGCCGTCCCGCCTGATAGAGCGTGGTATCGCGGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATATTGCGAACATCTGCCACTGTATCGTCagag                                                                                                                                    <  1:2192636/150‑1 (MQ=14)

                     cGGGTAAAAAAAACCTGTACAAAATGTGACTGTATTGTTGAAGCACCGGCGCCGTCCCGCCCGATAGAGCGTGGTATCGCGGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGt                                                                                                                                   <  1:1872683/150‑1 (MQ=14)

                       ggTAAAAAAAGCCTGTACAAAATGTGACTGTATTGTTGAAGCACCGGCGCCGTCCCGCCCGATAGAGCGTGGTATCGCGGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGa                                                                                                                                 <  1:1377721/150‑1 (MQ=17)

                        gTAAAAAAAACCTGTACAAAATGTGACTGTATTGTTGAAGCACCGGCGCCGTCCCGCCCGATATAGCGTGGTATCGCGGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGaa                                                                                                                                <  1:1845165/150‑1 (MQ=17)

                         tAAAAAAAGCCTGTACAAAATGTGACTGTATTGTTGAAGCACCGGCGCCGTCCCGCCCGATAGAGCGTGGTATCGCGGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGaaa                                                                                                                               >  1:2553854/1‑150 (MQ=17)

                           aaaaaaGCCTGTACAAAATGTGACTGTATTGTTGAAGCACCGGCGCCGTCCCGCCCGATAGAGCGTGGTATCGCGGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGaaa                                                                                                                               >  1:4001817/1‑148 (MQ=17)

                           aaaaaaGCCTGTACAAAATGTGACTGTATTGTTGAAGCACCGGCGCCGTCCCGCCCGATAGAGCGTGGTATCGCGGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATc                                                                                                                             >  1:774602/1‑150 (MQ=17)

                           aaaaaaGCCTGTACAAAATGTGACTGTATTGTTGAAGCACCGGCGCCGTCCCGCCCGATAGAGCGTGGTATCGCGGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATc                                                                                                                             <  1:815542/150‑1 (MQ=17)

                            aaaaaGCCTGTACAAAATGTGACTGTATTGTTGAAGCACCGGCGCCGTCCCGCCCGATAGAGCGTGGTATCGCGGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCt                                                                                                                            >  1:2348048/1‑150 (MQ=17)

                             aaaaGCCTGTACAAAATGTGACTGTATTGTTGAAGCACCGGCGCCGTCCCGCCCGATAGAGCGTGGTATCGCGGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCt‑t                                                                                                                          <  1:3201845/150‑1 (MQ=17)

                               aaGCCTGTACAAAATGTGACTGTATTGTTGAAGCACCGGCGCCGTCCCGCCCGATAGAGCGTGGTATCGCGGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCT‑TTg                                                                                                                        <  1:4131275/150‑1 (MQ=17)

                                agcctgtACAAAATGTGACTGTATTGTTGAAGCACCGGCGCCGTCCCGCCCGATAGAGCGTGGTATCGCGGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCT‑TTGc                                                                                                                       <  1:1466053/146‑1 (MQ=17)

                                 gcctgtACAAAATGTGACTGTACTGTTGAAGCACCGGCGCCGTCCCGCCCGATAGAGCGTGGTATCGCGGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCT‑TTGcc                                                                                                                      >  1:2785559/4‑150 (MQ=17)

                                    tgtACAAAATGTGACTGTATCGTTGAAGCACCGGCGCCGTCCCGCCCGATAGAGCGTGGTATCGCGGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCT‑TTGCCCgc                                                                                                                   >  1:178542/1‑149 (MQ=255)

                                     gtACAAAATGTGACTGCATCGTTGAAGCACCGGCGCCGTCCCGCCCGATAGAGCGTGGTATCGCGGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACACCTGCCACTGTATCGTCAGAGTGAAATCT‑TTGCCCGAc                                                                                                                  <  1:1734527/150‑1 (MQ=255)

                                       aCAAAATGTGACTGTATTGTTGAAGCACCGGCGCCGTCCCGCCCGATAGAGCGTGGTATCGCGGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCT‑TTGCCCGCCAg                                                                                                                >  1:3768643/1‑150 (MQ=18)

                                         aaaaTGTGACTGTATTGTTGAAGCACCGGCGCCGTCCCGCCCGATAGAGCGTGGTATCGCGGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCa                                                                                                                                       >  1:1051736/1‑126 (MQ=255)

                                          aaaTGTGACTGTATTGTTGAAGCACCGGCGCCGTCCCGCCCGATAGAGCGTGGTATCGCGGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCT‑TTGCCCGCCAGGgt                                                                                                             <  1:3804333/150‑1 (MQ=18)

                                          aaaTGTGACTGTATTGTTGAAGCACCGGCGCCGTCCCGCCCGATAGAGCGTGGTATCGCGGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCT‑TTGCCCGCCAGGgt                                                                                                             >  1:577033/1‑150 (MQ=18)

                                          aaaTGTGACTGTATTGTTGAAGCACCGGCGCCGTCCCGCCCGATAGAGCGTGCTATCGCGGGCCCCGGATTACTTGCCTGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCT‑TTGCCCGCCAGGgt                                                                                                             >  1:700756/1‑150 (MQ=18)

                                             tgtgACTGTATTGTTGAAGCACCGGCGCCGTCCCGCCCGATAGAGCGTGGTATCGCGGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCT‑TTGCCCGCCAGGGTGTc                                                                                                          <  1:242034/150‑1 (MQ=18)

                                               tgACTGTATTGTTGAAGCACCGGCGCCGTCCCGCCCGATAGAGCGTGGTATCGCGGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCT‑TTGCCCGCCAGGGTGTCGa                                                                                                        <  1:3556361/150‑1 (MQ=21)

                                               tgACTGTATTGTTGAAGCACCGGCGCCGTCCCGCCCGATAGAGCGTGGTATCGCGGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCT‑TTGCCCGCCAGGGTGTCGa                                                                                                        <  1:2304543/150‑1 (MQ=21)

                                                gACTGTATTGTTGAAGCACCGGCGCCGTCCCGCCCGATAGAGCGTGGTATCGCGGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCT‑TTGCCCGCCAGGGTGTCGaa                                                                                                       <  1:3477970/150‑1 (MQ=21)

                                                gACTGCATCGTTGAAGCACCGGCGCCGTCCCGCCCGATAGAGCGTGGTATCGCGGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACACCTGCCACTGTATCGTCAGAGTGAAATCT‑TTGCCCGACAGGGTGTCGaa                                                                                                       >  1:1702383/1‑150 (MQ=255)

                                                 actgtattgTTGAAGCAGCGGCGCCGTCCCGCCCGATAGAGCGTGGTATCGCGGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCT‑TTGCCCGCCAGGGTGTCGAAc                                                                                                      >  1:2224753/9‑150 (MQ=21)

                                                 actgtattgTTGAAGCACCGGCGCCATCCCGCCCGATAGAGCGTGGTATCGCGGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCT‑TTGCCCGCCAGGGTGTCGAAc                                                                                                      <  1:1258675/142‑1 (MQ=21)

                                                  ctgtattgTTGAAGCACCGGCGCCGTCCCGCCCGATAGAGCGTGGTATCGCGGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCT‑TTGCCCGCCAGGGTGTCGAACt                                                                                                     >  1:1498600/8‑150 (MQ=21)

                                                  ctgtattgTTGAAGCACCGGCGCCGTCCCGCCCGATAGAGCGTGGTATCGCGGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCT‑TTGCCCGCCAGGGTGTCGAACt                                                                                                     >  1:45045/8‑150 (MQ=21)

                                                   tgtattgTTGAAGCACCGGCGCCGTCCCGCCCGATAGAGCGTGGTATCGCGGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCT‑TTGCCCGCCAGGGTGTCGAACTg                                                                                                    <  1:2164806/144‑1 (MQ=255)

                                                    gtattgTTGAAGCACCGGCGCCGTCCCGCCCGATAGAGCGTGGTATCGCGGGCCCCGGATTACTTGCCCGCATGTTAACGGGAAAATACTGCGAACATCTGcc                                                                                                                                                   >  1:4069881/6‑103 (MQ=14)

                                                     tattgTTGAAGCACCGGCGCCGTCCCGCCCGATAGAGCGTGGTATCGCGGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCATTGTATCGTCAGAGTGAAATCT‑TTGCCCGCCAGGGTGTCGAACTGAg                                                                                                  <  1:2938214/146‑1 (MQ=21)

                                                        tgTTGAAGCACCGGCGCCGTCCCGCCCGATAGAGCGTGGTATCGCGGGCCCAAGATTCCTTGCCCCCTTTTTCACGGGAAAACACCGCAAACCTCTGCCACTGTCTCGTCAGAATGAAATCTCTTCCCCCACGGGGT‑TCGAACCGAGcca                                                                                               >  1:1942845/2‑149 (MQ=9)

                                                         gTTGAAGCACCGGCGCCGTCCCGCCCGATAGAGCGTGGTATCGCGGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACACCTGCCACTGTATCGTCAGAGTGAAATCT‑TTGCCCGACAGGGTGTCGAACTGAGCCGt                                                                                              >  1:1907953/1‑150 (MQ=255)

                                                          ttGAAGCACCGGCGCCGTCCCGCCCGATAGAGCGTGGTATCGCGGGCTCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCT‑TTGCCCGCCAGGGTGTCGAACTGAGCCggg                                                                                             <  1:3314146/150‑3 (MQ=255)

                                                           tGAAGCACCGGCGCCGTCCCGCCCGATAGAGCGTGGTATCGCGGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCT‑TTGCCCGCCAGGGTGTCGAACTGAGCCgggc                                                                                            <  1:1006269/150‑4 (MQ=255)

                                                            gAAGCACCGGCGCCGTCCCGCCCGATAGAGCGTGGTATCGCGGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCT‑TTGCCTGCCAGGGTGTCGAACTGAGCCgggcc                                                                                           >  1:168107/1‑146 (MQ=25)

                                                            gAAGCACCGGCGCCGTCCCGCCCGATAGAGCGTGGTATCGCGGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCT‑TTGCCCGCCAGGGTGTCGAACTGAGCCgggcc                                                                                           <  1:2876015/150‑5 (MQ=255)

                                                              aGCACCGGCGCCGTCCCGCCCGATAGAGCGTGGTATCGCGGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCT‑TTGCCCGCCAGGGTGTCGAACTGAGCCgggcctt                                                                                         >  1:1090115/1‑144 (MQ=255)

                                                              aGCACCGGCGCCGTCCCGCCCGATAGAGCGTGGTATCGCGGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACCGCGAACACCTGCCACTGTATCGTCAGAGTGAAATCT‑TTGCCCGACAGGGTGTCGAACTGAGCCGTGCAtt                                                                                         >  1:1126808/1‑150 (MQ=255)

                                                                cACCGGCGCCGTCCCGCCCGATAGAGCGTGGTATCGCGGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCT‑TTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTAc                                                                                       <  1:3523491/150‑1 (MQ=25)

                                                                cACCGGCGCCGTCCCGCCCGATAGAGCGTGGTATCGCGGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCT‑TTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTAc                                                                                       <  1:3254363/150‑1 (MQ=25)

                                                                 aCCGGCGCCGTCCCGCCCGATAGAGCGTGGTATCGCGGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCT‑TTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTAct                                                                                      <  1:2521140/150‑1 (MQ=25)

                                                                   cGGCGCCGTCCCGCCCGATAGAGCGTGGTATCGCGGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCT‑TTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTActct                                                                                    <  1:1823789/150‑1 (MQ=25)

                                                                     gcgcCGTCCCGCCCGATAGAGCGTGGTATCGCGGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCT‑TTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTActcccc                                                                                  >  1:2735399/1‑147 (MQ=25)

                                                                     gcgcCGTCCCGCCCGATAGAGCGTGGTATCGCGGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCT‑TTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTcc                                                                                  <  1:207049/150‑1 (MQ=37)

                                                                         cGTCCCGCCCGATAGAGCGTGGTATCGCGGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACACCTGCCACTGTATCGTCAGAGTGAAATCT‑TTGCCCGACAGGGTGTCGAACTGAGCCGTGCATTACTCTCCAACt                                                                              >  1:3750163/1‑150 (MQ=255)

                                                                           tCCCGCCCGATAGAGCGTGGTATCGCGGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCT‑TTGCCCGCCAGGGTGTCGAACTGAGTCGGGCCTTACTCTCCAACTgg                                                                            >  1:3577284/1‑150 (MQ=37)

                                                                           tCCCGCCCGATAGAGCGTGGTATCGCGGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCT‑TTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTgg                                                                            <  1:2496533/150‑1 (MQ=37)

                                                                            cccgcccgATAGAGCGTGGTATCGCGGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCT‑TTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTggg                                                                           >  1:3887695/1‑150 (MQ=37)

                                                                               gcccgATAGAGCGTGGTATCGCGGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCT‑TTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTg                                                                        >  1:1543035/1‑150 (MQ=37)

                                                                               gcccgATAGAGCGTGGTATCGCGGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCT‑TTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTg                                                                        >  1:3598154/1‑150 (MQ=37)

                                                                               gcccgATAGAGCGTGGTATCGCGGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCT‑TTGCCCGCCAGGGTGTCGAACTGAGCCAGGCCTTACTCTCCAACTGGGTTg                                                                        >  1:3835908/1‑150 (MQ=37)

                                                                                       gagCGTGGTATCGCGGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCT‑TTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGtgc                                                                <  1:954276/150‑1 (MQ=38)

                                                                                           gTGGTATCGCGGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCT‑TTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGtg                                                                 <  1:2374030/145‑1 (MQ=38)

                                                                                           gTGGTATCGCGGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCT‑TTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGcc                                                            <  1:866576/150‑1 (MQ=38)

                                                                                              gTATCGCGGGCCCCGGATTNCTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCT‑TTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGt                                                         >  1:4237183/1‑150 (MQ=39)

                                                                                               tATCGCGGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACACCTGCCACTGTATCGTCAGAGTGAAATCT‑TTGCCCGACAGGGTGTCGAACTGAGCCGTGCATTACTCTCCAACTGGGTTGACGCGTGCTGCCAGtt                                                        >  1:3294701/1‑150 (MQ=255)

                                                                                                aTCGCGGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCT‑TTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTa                                                       <  1:1299268/150‑1 (MQ=39)

                                                                                                 tCGCGGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCT‑TTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTaa                                                      >  1:1975730/1‑150 (MQ=39)

                                                                                                  cgcgGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCT‑TTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAAt                                                     >  1:4166722/1‑150 (MQ=39)

                                                                                                  cgcgGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCT‑TTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAAt                                                     <  1:2097573/150‑1 (MQ=39)

                                                                                                   gcgGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCT‑TTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATg                                                    <  1:514026/150‑1 (MQ=255)

                                                                                                   gcgGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCT‑TTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATg                                                    >  1:2273943/1‑150 (MQ=255)

                                                                                                     gggCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCT‑TTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGAc                                                  >  1:3028203/1‑150 (MQ=255)

                                                                                                              aTTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCT‑TTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGaa                                         <  1:2442131/150‑1 (MQ=255)

                                                                                                                 aCTTGCCCGCGTGTTAACAGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCT‑TTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAatga                                      <  1:1337826/150‑1 (MQ=255)

                                                                                                                    tGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCT‑TTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGc                                   <  1:616547/150‑1 (MQ=255)

                                                                                                                    tGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCT‑TTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGc                                   >  1:403648/1‑150 (MQ=255)

                                                                                                                      cccGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCT‑TTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGccc                                 >  1:2847856/1‑150 (MQ=255)

                                                                                                                        cgcgTGTTAACGGGAAAATACTGCGAACACCTGCCACTGTATCGTCAGAGTGAAATCT‑TTGCCCGACAGGGTGTCGAACTGAGCCGTGCATTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACGCCGCTGAATGATGCCCTg                               <  1:3966168/150‑1 (MQ=255)

                                                                                                                             gtTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCT‑TTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCCGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCg                          <  1:1176022/150‑1 (MQ=255)

                                                                                                                             gtTAACGGGAAAATACTGCGAACACCTGCCACTGTATCGTCAGAGTGAAATCT‑TTGCCCGACAGGGTGTCGAACTGAGCCGTGCATTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACGCCGCTGAATGATGCCATGTACCg                          <  1:2120074/150‑1 (MQ=255)

                                                                                                                               tAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCT‑TTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGtt                        >  1:3065078/1‑150 (MQ=255)

                                                                                                                                aaCGGGAAAATACTGCGAACATCTGCCACTGTATTGTCAGAGTGAAATCG‑TTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTa                       <  1:343451/150‑1 (MQ=255)

                                                                                                                                aaCGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCT‑TTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTa                       >  1:2608704/1‑150 (MQ=255)

                                                                                                                                aaCGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCT‑TTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGAAACCGGTGAATGATGCCCTGTAc                            >  1:2683434/1‑145 (MQ=255)

                                                                                                                                  cGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCT‑TTGCCCGCCAGGGTGTCGAACTGAGTCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTAtg                     >  1:3865485/1‑150 (MQ=255)

                                                                                                                                  cGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCT‑TTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTAtg                     <  1:1572833/150‑1 (MQ=255)

                                                                                                                                  cGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCT‑TTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTAtg                     >  1:3393278/1‑150 (MQ=255)

                                                                                                                                  cGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCT‑TTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGCTAATGACAACGGTGAATGATGCCCTGTACCGTTAtg                     >  1:3924425/1‑150 (MQ=255)

                                                                                                                                    ggAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCT‑TTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTAtgta                   >  1:3674691/1‑149 (MQ=255)

                                                                                                                                     gAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCT‑TTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTAtgtaa                  >  1:2986/1‑148 (MQ=255)

                                                                                                                                       aaaTACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCT‑TTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGtaatg                <  1:1429787/150‑1 (MQ=255)

                                                                                                                                        aaTACTGCGAACACCTGCCACTGTATCGTCAGAGTGAAATCT‑TTGCCCGACAGGGTGTCGAACTGAGCCGTGCATTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACGCCGCTGAATGATGCCCTGTACCGTTATGtgatga               >  1:4200397/1‑150 (MQ=255)

                                                                                                                                        aaTACTGCGAACACCTGCCACTGTATCGTCAGAGTGAAATCT‑TTGCCCGACAGGGTGTCGAACTGAGCCGTGCATTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACGCCGCTGAATGATGCCCTGTACCGTTATGtgatga               <  1:2024748/150‑1 (MQ=255)

                                                                                                                                            cTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCT‑TTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTATTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGaacac           >  1:746822/1‑147 (MQ=255)

                                                                                                                                            cTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCT‑TTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATAc           >  1:3358575/1‑150 (MQ=255)

                                                                                                                                            cTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCT‑TTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATAc           >  1:1612769/1‑150 (MQ=255)

                                                                                                                                            cTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCT‑TTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATAc           >  1:3118533/1‑150 (MQ=255)

                                                                                                                                            cTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCT‑TTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATAc           >  1:322868/1‑150 (MQ=255)

                                                                                                                                            cTGCGAACACCTGCCACTGTATCGTCAGAGTGAAATCT‑TTGCCCGACAGGGTGTCGAACTGAGCCGTGCATTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACGCCGCTGAATAATGCCCTGTACCGTTATGTGATGAATa            <  1:2386076/149‑1 (MQ=255)

                                                                                                                                              gCGAACATCTGCCACTGTATCGTCAGAGTGAAATCT‑TTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATAccc         >  1:1202214/1‑150 (MQ=255)

                                                                                                                                              gCGAACATCTGCCACTGTATCGTCAGAGTGAAATCT‑TTGCCCGCCAGGGTGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATAccc         >  1:799387/1‑150 (MQ=255)

CTGAAAGTGATCCGCACAGAACGGGTAAAAAAAGCGTGTACAAAATGTGACTGCATCGTTGAAGCACCGGCGCCGTCCCGCCCGATAGAGCGTGGTATCGCGGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACACCTGCCACTGTATCGTCAGAGTGAAATCT‑TTGCCCGACAGGGTGTCGAACTGAGCCGTGCATTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACGCCGCTGAATGATGCCCTGTACCGTTATGTGATGAATACCCGCAAGCT  >  Xen14_143/445‑743

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`