Predicted mutation
evidence seq id position mutation annotation gene description
RA Xen14_143 136 G→A S57S (AGC→AGT)  Xen14_04759 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Xen14_1431360GA80.1% 1561.8 / inf 895S57S (AGC→AGT) Xen14_04759hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (83/95);  new base A (344/373);  total (427/468)
Fisher's exact test for biased strand distribution p-value = 8.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 900 total aligned reads displayed.

‑‑‑‑‑‑‑‑‑‑CATTGCAGAACTGGAAAGCCAGCTCCGTCAGAAAGACCAGCAACTGAGTCTGGTTGAAGAGACGGAGGCCTTCCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGGCTCAGATAGAAAAACTGCGCCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACAGGCTGAGGCCCTGCTGAAACAACGCGAACAGGACAGCGATCGTTACAGTGGGCGGGAAGACGATCCACAGGTTCCCCG  >  Xen14_143/1‑284
                                                                                                                                                 |                                                                                                                                                    
tcgcattgcagaactggaaagccagctccgtcagaaagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCa                                                                                                                                                  >  1:3831311‑M1/84‑150 (MQ=255)
tcgcattgcagaactggaaagccagctccgtcagaaagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCa                                                                                                                                                  <  1:2348322‑M1/67‑1 (MQ=255)
tcgcattgcagaactggaaagccagctccgtcagaaagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCa                                                                                                                                                  <  1:3488775‑M1/67‑1 (MQ=255)
 cctcattcgcATTGCAGAACTGGAAAGCCAGCTCCGTCAGAAAGACCAGCAACTGAGTCTGGTTGAAGAGAAGGAGGCCTCCCTGCACTGGCACGTGCCGAAGAAGAGATCGAAGAAGATGAAAGGGAAATAGAACATCTGCGGGCTCAg                                                                                                                                                 >  1:3317023/10‑150 (MQ=255)
  gcattgcagaactggaaagccagctccgtcagaaagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGa                                                                                                                                                <  1:1608907‑M1/69‑1 (MQ=255)
   cattgcagaactggaaagccagctccgtcagaaagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGAt                                                                                                                                               <  1:3106361‑M1/70‑1 (MQ=255)
    cattcgcATTGCAGAACTGGAAAGCCAGCTCCGTCAGAAAGACCAGCAACTGAGTCTGGTTGAAGAGACGGAGGCCTTCCTGCACTGGCACGTGCCGAAGAAAAGGTCGAAGAAGATGAACGGGAAATAGAACATCTGCGGGCTCAGATa                                                                                                                                              <  1:303728/144‑1 (MQ=255)
     ttgcagaactggaaagccagctccgtcagaaagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAg                                                                                                                                             >  1:1818232‑M1/79‑150 (MQ=255)
       gcagaactggaaagccagctccgtcagaaagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGaa                                                                                                                                           <  1:2197744‑M1/74‑1 (MQ=255)
        cgcATTGCAGAACTGGAAAGCCAGCTCCGTCAGAAAGACCAGCAACTGAGTCTGGTTGAAGAGACGGAGGCCTTCCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGGCTCAGATAGaaa                                                                                                                                          <  1:3305047/148‑1 (MQ=255)
          gaactggaaagccagctccgtcagaaagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAg                                                                                                                                             <  1:753385‑M1/72‑1 (MQ=255)
           aactggaaagccagctccgtcagaaagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAAc                                                                                                                                       >  1:3537824‑M1/73‑150 (MQ=255)
           aactggaaagccagctccgtcagaaagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAAc                                                                                                                                       >  1:3679193‑M1/73‑150 (MQ=255)
            actggaaagccagctccgtcagaaagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACt                                                                                                                                      >  1:3599154‑M1/72‑150 (MQ=255)
                gaaagccagctccgtcagaaagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAActgcac                                                                                                                                  <  1:307541‑M1/83‑3 (MQ=255)
                  aaCTGGAAAGCCAGCTCCGTCAGAAAGACCAGCAACTGAGTCTGGTTGAAGAGACGGAGGCCTTCCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGGCTCAGATAGAAAAACTGCGCCg                                                                                                                                <  1:1406341/150‑1 (MQ=255)
                   agccagctccgtcagaaagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGTCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCgg                                                                                                                               >  1:876303‑M1/65‑150 (MQ=255)
                    gccagctccgtcagaaagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGa                                                                                                                              <  1:576659‑M1/87‑1 (MQ=255)
                    gccagctccgtcagaaagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGa                                                                                                                              <  1:2747397‑M1/87‑1 (MQ=255)
                     ccagctccgtcagaaagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGAt                                                                                                                             >  1:1316757‑M1/63‑150 (MQ=255)
                        gctccgtcagaaagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCt                                                                                                                          >  1:2596380‑M1/60‑150 (MQ=255)
                         ctccgtcagaaagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTg                                                                                                                         <  1:421276‑M1/92‑1 (MQ=255)
                          tccgtcagaaagaccagcaactgagtctggttgaagggacggaggccttcctgcgctCTGCACTGGCACATGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGt                                                                                                                        <  1:1000474‑M1/93‑1 (MQ=255)
                           ccgtcagaaagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAActgcac                                                                                                                                  <  1:2686155‑M1/83‑3 (MQ=255)
                            cgtcagaaagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTc                                                                                                                      >  1:731251‑M1/56‑150 (MQ=255)
                             gtcagaaagaccagcaactgagtctggttgaagagacggaggccttcctgcgttCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCg                                                                                                                     <  1:1917129‑M1/96‑1 (MQ=255)
                             gtcagaaagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCg                                                                                                                     <  1:194378‑M1/96‑1 (MQ=255)
                              tcagaaagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCgg                                                                                                                    >  1:487087‑M1/54‑150 (MQ=255)
                               cagaaagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGt                                                                                                                   <  1:3348280‑M1/98‑1 (MQ=255)
                               cagaaagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGt                                                                                                                   >  1:286084‑M1/53‑150 (MQ=255)
                                agaaagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGCCAGAAAAACTGCACCGGATGCTGTTCGGTa                                                                                                                  <  1:3655160‑M1/99‑1 (MQ=255)
                                agaaagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTa                                                                                                                  <  1:2969373‑M1/99‑1 (MQ=255)
                                agaaagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTa                                                                                                                  <  1:1131138‑M1/99‑1 (MQ=255)
                                 gaaagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTAc                                                                                                                 <  1:2139645‑M1/100‑1 (MQ=255)
                                 gaaagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTAc                                                                                                                 <  1:1546262‑M1/100‑1 (MQ=255)
                                   aagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGNAGAAGATGAACGGNAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTAccc                                                                                                               <  1:3721240‑M1/102‑1 (MQ=255)
                                    agaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCt                                                                                                                          <  1:4145799‑M1/91‑1 (MQ=255)
                                       ccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTc                                                                                                           <  1:1679333‑M1/106‑1 (MQ=255)
                                       ccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTc                                                                                                           <  1:3134539‑M1/106‑1 (MQ=255)
                                         agcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACTAGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTg                                                                                                         >  1:4073589‑M1/43‑150 (MQ=255)
                                         aaaGACCAGCAACTGAGTCTGGTTGAAGAGACGGAGGCCTTCCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGGCTCAGATAGAAAAACTGCGCCGGATGCTGTTCGGTACCCGTTCTg                                                                                                         <  1:2939663/150‑1 (MQ=255)
                                           caactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGaa                                                                                                       >  1:840329‑M1/41‑150 (MQ=255)
                                              ccAGCAACTGAGTCTGGTTGAAGAGAGGGAGGCCTTCCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGGCTCAGATAGAAAAACTGCGCCGGATGCTGTTCGGTACCCGTTCTg                                                                                                         >  1:1147524/1‑145 (MQ=255)
                                               tgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAAc                                                                                                   >  1:90300‑M1/37‑150 (MQ=255)
                                               tgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAAc                                                                                                   >  1:4031325‑M1/37‑150 (MQ=255)
                                                 agtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCTAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTg                                                                                                 <  1:152439‑M1/116‑1 (MQ=255)
                                                   tctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGcg                                                                                               >  1:3883463‑M1/33‑150 (MQ=255)
                                                     tggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGcgtc                                                                                             >  1:3960566‑M1/31‑150 (MQ=255)
                                                      ggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGcgtcg                                                                                            <  1:350513‑M1/121‑1 (MQ=255)
                                                      ggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGcgtcg                                                                                            <  1:3758699‑M1/121‑1 (MQ=255)
                                                         tgaagagacggatgccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGa                                                                                         >  1:620397‑M1/27‑150 (MQ=255)
                                                         tgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGa                                                                                         >  1:434155‑M1/27‑150 (MQ=255)
                                                           aagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAg                                                                                       <  1:1984345‑M1/126‑1 (MQ=255)
                                                           aagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAg                                                                                       >  1:3419788‑M1/25‑150 (MQ=255)
                                                            agagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGt                                                                                      >  1:3565592‑M1/24‑150 (MQ=255)
                                                            agagacggaggccctcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGATTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGt                                                                                      <  1:781967‑M1/127‑1 (MQ=255)
                                                             gagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGCAATAGAACATCTGCGGACTCAGATCGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTAAAGtt                                                                                     <  1:2813544‑M1/128‑1 (MQ=255)
                                                             gagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGtt                                                                                     <  1:4050538‑M1/128‑1 (MQ=255)
                                                              agacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCAGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTg                                                                                    >  1:1354839‑M1/22‑150 (MQ=255)
                                                               gacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGa                                                                                   <  1:2399943‑M1/130‑1 (MQ=255)
                                                               gacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGa                                                                                   <  1:139158‑M1/130‑1 (MQ=255)
                                                                 gAAGAGACGGAGGCCTTCCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGGCTCAGATAGAAAAACTGCGCCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAAc                                                                                 <  1:3001224/150‑1 (MQ=255)
                                                                 cggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTGTGAAAAACTGCGTCGTGAAGTTGAAc                                                                                 <  1:2486460‑M1/132‑1 (MQ=255)
                                                                 cggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAAc                                                                                 <  1:2638158‑M1/132‑1 (MQ=255)
                                                                  ggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAact                                                                                >  1:1499489‑M1/18‑149 (MQ=255)
                                                                  ggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAact                                                                                <  1:3262818‑M1/133‑2 (MQ=255)
                                                                    aggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTgaactgg                                                                              <  1:1460752‑M1/135‑4 (MQ=255)
                                                                     ggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTtgaactggc                                                                             >  1:379404‑M1/15‑146 (MQ=255)
                                                                     agaCGGAGGCCTTCCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGGCTCAGATAGAAAAACTGCGCCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACAGGc                                                                             <  1:2516615/150‑1 (MQ=255)
                                                                      gccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACTGGCt                                                                            >  1:3222813‑M1/14‑150 (MQ=255)
                                                                       ccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACTGGCTg                                                                           >  1:1288755‑M1/13‑150 (MQ=255)
                                                                         ttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAGGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACTGGCTGAg                                                                         >  1:2770658‑M1/11‑150 (MQ=255)
                                                                         ttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAGATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACTGGCTGAg                                                                         >  1:231828‑M1/11‑150 (MQ=255)
                                                                         ttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACTGGCTGAg                                                                         >  1:2288883‑M1/11‑150 (MQ=255)
                                                                           aGGCCTTCCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGGCTCAGATAGAAAAACTGCGCCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACAGGCTGAGGc                                                                       <  1:1710798/150‑1 (MQ=255)
                                                                            ctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACTGGCTGAGgct                                                                      <  1:3922129‑M1/143‑2 (MQ=255)
                                                                                gctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACTGGCTGAGGCTctgc                                                                  >  1:1853138‑M1/4‑150 (MQ=255)
                                                                                 ctCTGCACTGGCACGTGCCGAAGAAAAGGTCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACTGGCTGAGGCTctgct                                                                 >  1:2001962‑M1/3‑150 (MQ=255)
                                                                                 ctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACTGGCTGAGGCTctgct                                                                 >  1:1240235‑M1/3‑150 (MQ=255)
                                                                                  tCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACTGGCTGAGGCTctgctg                                                                >  1:684964‑M1/2‑150 (MQ=255)
                                                                                   cTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACTGGCTGAGGCTCTGCTGa                                                               <  1:3386680/150‑1 (MQ=25)
                                                                                    tGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGGCTCAGATAGAAAAACTGCGCCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACAGGCTGAGGCCCTGCTGaa                                                              <  1:2439356/150‑1 (MQ=255)
                                                                                    tGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGGCTCAGATAGAAAAACTGCGCCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACAGGCTGAGGCCCTGCTGaa                                                              <  1:2040568/150‑1 (MQ=255)
                                                                                     gCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACTGGCTGAGGCTCTGCTGaaa                                                             >  1:2881823/1‑150 (MQ=25)
                                                                                     gCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACTGGCTGAGGCTCTGCTGaaa                                                             <  1:3175298/150‑1 (MQ=25)
                                                                                      taCTGGCACGTGCCGAAGAAAAGATCTAAGAAGATGTACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACTGGCTGAGGCTCTGCTGAaac                                                            <  1:964265/149‑1 (MQ=21)
                                                                                      cACTGGCGCGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACTGGCTGAGGCTCTGCTGAaac                                                            <  1:2064999/150‑1 (MQ=25)
                                                                                        cTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACTGGCTGAGGCTCTGCTGAaacaa                                                          >  1:532999/1‑150 (MQ=25)
                                                                                         tGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACTGGCTGAGGCTCTGCTGAaacaac                                                         <  1:3630213/150‑1 (MQ=25)
                                                                                          ggCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACTGGCTGAGGCTCTGCTGAAACAAcg                                                        <  1:17729/150‑1 (MQ=25)
                                                                                                 gCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGGCTCAGATAGAAAAACTGCGCCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACAGGCTGAGGCCCTGCTGAAACAACGCGAACAg                                                 >  1:2539051/1‑150 (MQ=255)
                                                                                                    gaagaaAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGGCTCAGATAGAAAAACTGCGCCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACAGGCTGAGGCCCTGCTGAAACAACGCGAACAGGAc                                              <  1:2114246/150‑1 (MQ=255)
                                                                                                    gaagaaAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGGCTCAGATAGAAAAACTGCGCCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACAGGCTGAGGCCCTGCTGAAACAACGCAAACAGGAc                                              >  1:2251175/1‑150 (MQ=255)
                                                                                                      agaaAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGGCTCAGATAGAAAAACTGCGCCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACAGGCTGAGGCCCTGCTGAAACAACGCGAACAGGACAg                                            >  1:1794345/1‑150 (MQ=255)
                                                                                                            gATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGGCTCAGATAGAAAAACTGCGCCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACAGGCTGAGGCCCTGCTGAAACAACGCGAACAGGACAGCGATCg                                      >  1:1660640/1‑150 (MQ=255)
                                                                                                             aTCGAAGAAGATGAACGGGAAATAGAACATCTGCGGGCGCAGATAGAAAAACTGCGCCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACAGGCTGAGGCCCTGCTGAAACAACGCGAACAGGACAGCGATCgc                                     <  1:3844920/150‑2 (MQ=255)
                                                                                                                             gggAAATAGAACATCTGCGGGCTCAGATAGAAAAACTGCGCCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACAGGCTGAGGCCCTGCTGAAACAACGCGAACAGGACAGCGATCGTTACAGTGGGCGGGAAg                     <  1:3458608/150‑1 (MQ=255)
                                                                                                                                 aaTAGAACATCTGCGGGCTCAGATAGAAAAACTGCGCCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACAGGCTGAGGCCCTGCTGAAACAACGCGAACAGGACAGCGATCGTTACAGTGGGCGGGAAGACGa                 >  1:1187906/1‑150 (MQ=255)
                                                                                                                                             gCGGGCTCAGATAGAAAAACTGCGCCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACAGGCTGAGGCCCTGCTGAAACAACGCGAACAGGACAGCGATCGTTACAGTGGGCGGGAAGa                    >  1:1044998/1‑135 (MQ=255)
                                                                                                                                                ggCTCAGATAGAAAAACTGCGCCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACAGGCTGAGGCCCTGCTGAAACAACGCGAACAGGACAGCGATCGTTACAGTGGGCGGGAAGACGATCCACAGGTTCCCCg  >  1:653829/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
‑‑‑‑‑‑‑‑‑‑CATTGCAGAACTGGAAAGCCAGCTCCGTCAGAAAGACCAGCAACTGAGTCTGGTTGAAGAGACGGAGGCCTTCCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGGCTCAGATAGAAAAACTGCGCCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACAGGCTGAGGCCCTGCTGAAACAACGCGAACAGGACAGCGATCGTTACAGTGGGCGGGAAGACGATCCACAGGTTCCCCG  >  Xen14_143/1‑284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑