BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	Xen14_126	2,053	G→T	P89P (CCG→CCT)	Xen14_04726 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Xen14_126	2,053	0	G	T	81.0%	2267.1 / inf	1027	P89P (CCG→CCT)	Xen14_04726	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (69/126); new base T (437/392); total (506/521)
Fisher's exact test for biased strand distribution p-value = 1.57e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01

Only 100 of 1041 total aligned reads displayed.

AATGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGACTTTGGCAAAACGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTT‑‑‑‑A‑‑‑‑‑‑TCCACCCAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTGGAAAATCCTTCACCAGAGCTGCTCACGGTGTTGATCCGCGAACTGAGTGGGAGGGGAAG  >  Xen14_126/1904‑2202

      gTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAAACTGTGgcg                                                                                                                                                           >  1:3922411/1‑149 (MQ=255)

       tCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcgg                                                                                                                                                          <  1:136332/150‑3 (MQ=255)

        cAATGATAATGTTATTTTCAAATGGCTCAGGCTCTNGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggc                                                                                                                                                         >  1:3894213/1‑147 (MQ=255)

        cAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggc                                                                                                                                                         >  1:1072023/1‑147 (MQ=255)

        cAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggc                                                                                                                                                         >  1:717588/1‑147 (MQ=255)

         aaTGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggcc                                                                                                                                                        <  1:3178440/150‑5 (MQ=255)

          aTGATAATGTTATTTTCAAATGGCTCAGACTTTGGCAAAACGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGctc                                                                                                                                                       >  1:1751437/1‑150 (MQ=255)

           tGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggccat                                                                                                                                                      <  1:1609395/150‑7 (MQ=255)

            gATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggccatt                                                                                                                                                     >  1:4017014/1‑143 (MQ=255)

             aTAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCATGTTTCGCGGCGTCTTCCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggccattg                                                                                                                                                    >  1:776635/1‑142 (MQ=255)

              tAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggccattgc                                                                                                                                                   >  1:1300896/1‑141 (MQ=255)

              tAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCATGTTTCGCGGCGTCTTCCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggccattgc                                                                                                                                                   <  1:745048/150‑10 (MQ=255)

                 tGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCAGGATGAGCAGAAAGAACCTGTGgcggccattgcgcc                                                                                                                                                >  1:1534090/1‑138 (MQ=255)

                  gTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggccattgcgcca                                                                                                                                               <  1:852387/150‑14 (MQ=255)

                  gTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggccattgcgcca                                                                                                                                               >  1:2410825/1‑137 (MQ=255)

                   ttATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggccattgcgccat                                                                                                                                              <  1:1128140/150‑15 (MQ=255)

                   ttATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggccattgcgccat                                                                                                                                              <  1:1703218/150‑15 (MQ=255)

                   ttATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCAGGATGAGCAGAAAGAACCTGTGgcggccattgcgccac                                                                                                                                              >  1:1000253/1‑136 (MQ=255)

                    tATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggccattgcgccatc                                                                                                                                             <  1:1439975/150‑16 (MQ=255)

                         tCAAATGGCTCAGACTTTGGCAAAACGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTT‑‑‑‑A‑‑‑‑‑‑t                                                                                                                              <  1:427401/150‑1 (MQ=255)

                           aaaTGGCTCAGGCTCTGGCAGAACGAAGGGCATGTTTCGCGGCGTCTTCCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTT‑‑‑‑A‑‑‑‑‑‑Tcc                                                                                                                            <  1:2824090/150‑1 (MQ=255)

                              tGGCTCAGGCTCTGACAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTT‑‑‑‑A‑‑‑‑‑‑TCCAct                                                                                                                         <  1:3809296/150‑2 (MQ=255)

                               ggCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑‑‑‑‑TCCActt                                                                                                                        <  1:462195/150‑3 (MQ=255)

                                gCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑‑‑‑‑TCCActtc                                                                                                                       >  1:1046114/1‑147 (MQ=255)

                                gCTCAGACTTTGGCAAAACGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTT‑‑‑‑A‑‑‑‑‑‑TCCACCCa                                                                                                                       <  1:133678/150‑1 (MQ=255)

                                 cTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTAACACTGGCGTTGAATTATTACCTGTAGAAATACCGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑‑‑‑‑TCCActtcc                                                                                                                      >  1:1173339/1‑146 (MQ=255)

                                     ggctctGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑‑‑‑‑TCCActtccactc                                                                                                                  <  1:4211183/145‑9 (MQ=255)

                                         ctGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑‑‑‑‑TCCActtccactcagac                                                                                                              >  1:1024647/2‑138 (MQ=255)

                                          tGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑‑‑‑‑TCCActtccactcagacc                                                                                                             <  1:1153924/150‑14 (MQ=255)

                                           ggCAGAACGAAGGGCGTGTTTCGCGGTGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑‑‑‑‑TCCActtccactcagacca                                                                                                            <  1:2462641/150‑15 (MQ=255)

                                            gCAAAACGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTT‑‑‑‑A‑‑‑‑‑‑TCCACCCAGACTACTGTCAg                                                                                                           >  1:1790830/1‑150 (MQ=255)

                                                      gggCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTT‑‑‑‑A‑‑‑‑‑‑TCCACCCAGACTACTGTCAGTGCCAGCTcc                                                                                                 <  1:546733/150‑1 (MQ=255)

                                                                cgcgGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCGGTCTTT‑‑‑‑A‑‑‑‑‑‑TCCACCCAGACTACTGTCAGTGCCAGCTCCTGCAAGATgg                                                                                       <  1:1924074/150‑1 (MQ=255)

                                                                cgcgGCGTCTTCCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTT‑‑‑‑A‑‑‑‑‑‑TCCACttccactcagaacagagtcagtgccagttcctgca                                                                                       >  1:2224379‑M1/1‑115 (MQ=255)

                                                                        cttccggtaACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTT‑‑‑‑A‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaa                                                                               >  1:1458529‑M1/5‑107 (MQ=255)

                                                                             ggTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccg                                                                          <  1:3035300‑M1/150‑49 (MQ=255)

                                                                             ggTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccg                                                                          <  1:3580045‑M1/150‑49 (MQ=255)

                                                                                 acaacCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTT‑‑‑‑A‑‑‑‑‑‑TCCACCCAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAAc                                                                      <  1:2767203/150‑1 (MQ=255)

                                                                                  cgacctCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacg                                                                     >  1:2189594‑M1/3‑97 (MQ=255)

                                                                                  cgacctCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacg                                                                     >  1:3853579‑M1/3‑97 (MQ=255)

                                                                                     ccTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggta                                                                  <  1:2323050‑M1/150‑57 (MQ=255)

                                                                                      ctctTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaa                                                                 >  1:12781‑M1/1‑93 (MQ=255)

                                                                                       tcttctGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaac                                                                <  1:2136975‑M1/150‑59 (MQ=255)

                                                                                       tcttctGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaac                                                                >  1:2544216‑M1/1‑92 (MQ=255)

                                                                                           ctGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatga                                                            >  1:2288126‑M1/1‑88 (MQ=255)

                                                                                            tGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgac                                                           <  1:1498768‑M1/150‑64 (MQ=255)

                                                                                             gACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTT‑‑‑‑A‑‑‑‑‑‑TCCACCCAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTGGaa                                                          <  1:1964425/150‑1 (MQ=255)

                                                                                             gACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATCGCGCCATCTTT‑‑‑‑A‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacg                                                          <  1:1091555‑M1/150‑65 (MQ=255)

                                                                                                acTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctg                                                       <  1:1577957‑M1/150‑68 (MQ=255)

                                                                                                acTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTGT‑‑‑‑AGCCACTTCCACGCAGACCGGAGTCAGTGACAGTTCCTGCAAGGTGGAATTCCGTCACGGTAACATGACGCTg                                                             <  1:2990752/150‑1 (MQ=255)

                                                                                                 cTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctgg                                                      >  1:980842‑M1/1‑82 (MQ=255)

                                                                                                   ggCGTTGAATTATTACCTGTAGAAATAACGCCGGCTGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTCTCCACT‑‑‑‑TCCACTCAGAACAGAGTCAGTGCCAGTTCCTTCAAGGTGGAATTCCGTCACGGTAACATGACGCTGGac                                                          >  1:3282906/1‑149 (MQ=255)

                                                                                                    gCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTT‑‑‑‑A‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaa                                                   >  1:3437966‑M1/1‑79 (MQ=255)

                                                                                                    gCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaa                                                   <  1:604490‑M1/150‑72 (MQ=255)

                                                                                                    gCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaa                                                   >  1:1907936‑M1/1‑79 (MQ=255)

                                                                                                      gTTGAATTATTACCTGTGGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaat                                                 >  1:2657926‑M1/1‑77 (MQ=255)

                                                                                                      gTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTT‑‑‑‑A‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaat                                                 <  1:399055‑M1/150‑74 (MQ=255)

                                                                                                      gTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaat                                                 >  1:2636283‑M1/1‑77 (MQ=255)

                                                                                                      gTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaat                                                 <  1:81259‑M1/150‑74 (MQ=255)

                                                                                                      gTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaat                                                 >  1:3497620‑M1/1‑77 (MQ=255)

                                                                                                       ttGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTT‑‑‑‑A‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatc                                                >  1:1851570‑M1/1‑76 (MQ=255)

                                                                                                       ttGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTT‑‑‑‑A‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctgga                                                     >  1:3620253‑M1/1‑76 (MQ=255)

                                                                                                        tGAATTATTACCTGTAGAAATAACGCCGGTTGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatcc                                               <  1:2091304‑M1/150‑76 (MQ=255)

                                                                                                        tGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACATGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatcc                                               >  1:4090629‑M1/1‑75 (MQ=255)

                                                                                                         gAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTT‑‑‑‑A‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatcca                                              >  1:2590080‑M1/1‑74 (MQ=255)

                                                                                                         gAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatcca                                              >  1:1810733‑M1/1‑74 (MQ=255)

                                                                                                         gAATTATTACCTGTAGAAACAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatcca                                              <  1:96138‑M1/150‑77 (MQ=255)

                                                                                                           attattACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTACTCTTACTCCGTCTTT‑‑‑‑A‑‑‑‑‑‑TCCACCCAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTGGAAAATCCTTCACCaga                                            <  1:296261/150‑1 (MQ=255)

                                                                                                           attattACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTT‑‑‑‑A‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatc                                            >  1:2881920‑M1/1‑72 (MQ=255)

                                                                                                           attattACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑‑‑‑‑CCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatcca                                              >  1:3376831‑M1/1‑72 (MQ=255)

                                                                                                           attattACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACATGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatc                                            <  1:2064916‑M1/150‑79 (MQ=255)

                                                                                                            ttattaCCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcg                                           >  1:3711203‑M1/1‑71 (MQ=255)

                                                                                                            ttattaCCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcg                                           <  1:1993234‑M1/150‑80 (MQ=255)

                                                                                                              attaCCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTT‑‑‑‑A‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgcc                                         <  1:1387263‑M1/150‑82 (MQ=255)

                                                                                                               ttaCCTGTAGACATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgcca                                        >  1:2031815‑M1/1‑68 (MQ=255)

                                                                                                                taCCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccag                                       <  1:643791‑M1/150‑84 (MQ=255)

                                                                                                                taCCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccag                                       >  1:584953‑M1/1‑67 (MQ=255)

                                                                                                                   ctgtagaaataacgccgGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagc                                    >  1:3762284‑M1/9‑64 (MQ=255)

                                                                                                                    tgtagaaataacgccGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagct                                   >  1:2924146‑M1/8‑63 (MQ=255)

                                                                                                                    tgtagaaataacgccGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagct                                   >  1:3084977‑M1/8‑63 (MQ=255)

                                                                                                                    tgtagaaataacgccGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagct                                   >  1:3663413‑M1/8‑63 (MQ=255)

                                                                                                                    tgtagaaataacgccGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagct                                   >  1:248912‑M1/8‑63 (MQ=255)

                                                                                                                     gtagaaataacgcCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑‑‑‑‑TCCACttccattcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctg                                  >  1:2376612‑M1/7‑62 (MQ=255)

                                                                                                                      tagaaataacgCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgc                                 >  1:1208363‑M1/6‑61 (MQ=255)

                                                                                                                       agaaataacGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgct                                <  1:887766‑M1/146‑91 (MQ=255)

                                                                                                                        gaaataaCGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgctc                               <  1:499324‑M1/147‑92 (MQ=255)

                                                                                                                             aaCGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTT‑‑‑‑A‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgctcacggt                          <  1:3107898‑M1/150‑97 (MQ=255)

                                                                                                                              aCGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgctcacggtg                         >  1:4149890‑M1/1‑53 (MQ=255)

                                                                                                                               cGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTT‑‑‑‑A‑‑‑‑‑‑TCCACCCAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTGGAAAATCCTTCACCAGAGCTGCTCACGGTGTTGATcc                        <  1:155591/150‑1 (MQ=255)

                                                                                                                               cGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTT‑‑‑‑A‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgctcacggtgt                        <  1:1634312‑M1/150‑99 (MQ=255)

                                                                                                                               cGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgctcacggtgt                        >  1:3243421‑M1/1‑52 (MQ=255)

                                                                                                                               cGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcaaggtaacatgacgctggaaaatccatcgccagagctgctcaaggtgt                        >  1:2700861‑M1/1‑52 (MQ=255)

                                                                                                                                gCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTT‑‑‑‑A‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgctcacggtgtt                       <  1:3542542‑M1/150‑100 (MQ=255)

                                                                                                                                gCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTT‑‑‑‑A‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgctcacggtgtt                       <  1:1674958‑M1/150‑100 (MQ=255)

                                                                                                                                      tGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTT‑‑‑‑A‑‑‑‑‑‑TCCACCCAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTGGAAAATCCTTCACCAGAGCTGCTCACGGTGTTGATCCGCGAACt                 <  1:928558/150‑1 (MQ=255)

                                                                                                                                        agcagaaagAACCTGTGGCGGCCATTGCGCCGTCTTT‑‑‑‑A‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgctcacgatgttaatccgcg               <  1:2351247‑M1/146‑108 (MQ=255)

                                                                                                                                         gcagaaaGAACCTGTGGCTGCCATTGCGCCATCTTT‑‑‑‑A‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgctcacggtgttgatccgtga              >  1:173250‑M1/4‑42 (MQ=255)

                                                                                                                                         gcagaaaGAACCTGTGGCGGCCATTGCGCCGTCTTT‑‑‑‑A‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgctcacggtgttgatccgtga              >  1:3468658‑M1/4‑42 (MQ=255)

                                                                                                                                         gCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTT‑‑‑‑A‑‑‑‑‑‑TCCACCCAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTGGAAAATCCTTCACCAGAGCTGCTCACGGTGTTGATCCGCGAACTGAg              >  1:3320722/1‑150 (MQ=255)

                                                                                                                                           cGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTT‑‑‑‑A‑‑‑‑‑‑TCCACCCAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTGGAAAATCCTTCACCAGAGCTGCTCACGGTGTTGATCCGCGa                    <  1:1229428/142‑1 (MQ=255)

AATGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGACTTTGGCAAAACGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTT‑‑‑‑A‑‑‑‑‑‑TCCACCCAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTGGAAAATCCTTCACCAGAGCTGCTCACGGTGTTGATCCGCGAACTGAGTGGGAGGGGAAG  >  Xen14_126/1904‑2202

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`