BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	Xen14_126	2,043	C→A	P86Q (CCG→CAG)	Xen14_04726 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Xen14_126	2,043	0	C	A	82.8%	2624.6 / inf	1122	P86Q (CCG→CAG)	Xen14_04726	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (72/121); new base A (479/449); total (551/571)
Fisher's exact test for biased strand distribution p-value = 3.53e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 1141 total aligned reads displayed.

TGCTCGAGAAAATGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGACTTTGGCAAAACGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTTA‑‑‑‑‑‑TCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTGGAAAATCCTTCACCAGAGCTGCTCACGGTGTTGATCCGCGAACTGAGTG  >  Xen14_126/1894‑2192

     gtgaAAATGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGTGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAg                                                                                                                                                              >  1:3907570/3‑150 (MQ=255)

     gagaAAATGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCATGTTTCGCGGCGTCTTCCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAg                                                                                                                                                              <  1:2204835/150‑1 (MQ=255)

      tgaAAATGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGa                                                                                                                                                             >  1:2292202/2‑150 (MQ=255)

      agaAAATGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGACTTTGGCAAAACGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGa                                                                                                                                                             <  1:4126449/150‑1 (MQ=255)

        aaaaTGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAAc                                                                                                                                                           <  1:1025385/150‑1 (MQ=255)

        aaaaTGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAAc                                                                                                                                                           <  1:1761066/150‑1 (MQ=255)

         aaaTGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCATGTTTCGCGGCGTCTTCCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAAcc                                                                                                                                                          <  1:1998238/150‑1 (MQ=255)

             ggCGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCtgtg                                                                                                                                                      >  1:598318/1‑150 (MQ=255)

             ggCGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCtgtg                                                                                                                                                      <  1:2164466/150‑1 (MQ=255)

               cGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgc                                                                                                                                                    >  1:2354855/1‑150 (MQ=255)

               cGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgc                                                                                                                                                    >  1:2826970/1‑150 (MQ=255)

                 tCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcgg                                                                                                                                                  <  1:136336/150‑3 (MQ=255)

                  cAATGATAATGTTATTTTCAAATGGCTCAGACTTTGGCAAAACGA‑GGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGgctgct                                                                                                                                                <  1:1793809/150‑1 (MQ=255)

                   aaTGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggcc                                                                                                                                                <  1:3515518/150‑5 (MQ=255)

                   aaTGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggcc                                                                                                                                                <  1:3958949/150‑5 (MQ=255)

                        tAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggccattgc                                                                                                                                           >  1:3235559/1‑141 (MQ=255)

                           tGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCAGGATGAGCAGAAAGAACCTGTGgcggccattgcgcc                                                                                                                                        >  1:1534090/1‑138 (MQ=255)

                           tGTTATTTTCAAATGGCTCAGACTTTGGCAAAACGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTcc                                                                                                                                        <  1:1209473/150‑1 (MQ=255)

                            gTTATTTTCAAATGGCTCAGGCTCTGGCAGAGCGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggccattgcgccg                                                                                                                                       >  1:1622185/1‑137 (MQ=255)

                            gTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggccattgcgcca                                                                                                                                       >  1:2665582/1‑137 (MQ=255)

                            gTTATTTTCAAATGGCTCAGACTTTGGCAAAACGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCg                                                                                                                                       <  1:1341476/150‑1 (MQ=255)

                             ttATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggccattgcgccat                                                                                                                                      <  1:1133742/150‑15 (MQ=255)

                             ttATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggccattgcgccat                                                                                                                                      <  1:951025/150‑15 (MQ=255)

                             ttATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggccattgcgccat                                                                                                                                      >  1:1863266/1‑136 (MQ=255)

                             ttATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCAGGATGAGCAGAAAGAACCTGTGgcggccattgcgccac                                                                                                                                      >  1:1000253/1‑136 (MQ=255)

                              tATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggccattgcgccacc                                                                                                                                     <  1:1439972/150‑16 (MQ=255)

                                ttttCAAATGGCTCAGACTTTGGCAAAACGAAGGGCGTACATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCtt                                                                                                                                   >  1:3997216/1‑150 (MQ=255)

                                 tttCAAATGGCTCAGACTTTGGCAAAACGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCtct                                                                                                                                  >  1:383278/1‑148 (MQ=255)

                                   tCAAATGGCTCAGGCTTTGGAAAAACGAAGGGCGTATATCGCGGCTTCTTTCGGTAACAACCTCCTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTTA‑‑‑‑‑‑t                                                                                                                          <  1:1941545/150‑1 (MQ=255)

                                   tCAAATGGCTCAGACTTTGGCAAAACGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTTA‑‑‑‑‑‑t                                                                                                                          <  1:427402/150‑1 (MQ=255)

                                       aTGGCTCAGGCTCTGGCAGAACGAAGGGCATGTTTCGCGGCGTCTTCCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTTA‑‑‑‑‑‑TCCAc                                                                                                                      <  1:358836/150‑1 (MQ=255)

                                          gCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTA‑‑‑‑‑‑TCCActtc                                                                                                                   >  1:1046114/1‑147 (MQ=255)

                                           cTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAGCGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTA‑‑‑‑‑‑TCCActtcc                                                                                                                  >  1:2713612/1‑146 (MQ=255)

                                           cTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTA‑‑‑‑‑‑TCCActtcc                                                                                                                  <  1:3556374/150‑5 (MQ=255)

                                           cTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTA‑‑‑‑‑‑TCCActtcc                                                                                                                  >  1:4046941/1‑146 (MQ=255)

                                              aggctctGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTA‑‑‑‑‑‑TCCActtccact                                                                                                               <  1:793798/144‑8 (MQ=255)

                                               ggctctGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTTA‑‑‑‑‑‑TCCActtccactc                                                                                                              <  1:1704639/145‑9 (MQ=255)

                                               ggctctGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTA‑‑‑‑‑‑TCCActtccactc                                                                                                              >  1:1094087/6‑142 (MQ=255)

                                                 ctctGGCAGAACGAAGGGCATGTTTCGCGGCGTCTTCCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTTA‑‑‑‑‑‑TCCActtccactcag                                                                                                            >  1:2327373/4‑140 (MQ=255)

                                                  tctGGCAGAACGAAGGGCGTGTTTCGCGGTGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTA‑‑‑‑‑‑TCCActtccactcaga                                                                                                           <  1:1233511/148‑12 (MQ=255)

                                                  tctGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTA‑‑‑‑‑‑TCCActtccactcaga                                                                                                           <  1:362703/148‑12 (MQ=255)

                                                   ctGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTA‑‑‑‑‑‑TCCActtccactcagac                                                                                                          <  1:197142/149‑13 (MQ=255)

                                                   ctGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTA‑‑‑‑‑‑TCCActtccactcagac                                                                                                          <  1:2245911/149‑13 (MQ=255)

                                                   ctGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTA‑‑‑‑‑‑TCCActtccactcagac                                                                                                          >  1:511579/2‑138 (MQ=255)

                                                       cAAAACGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTTA‑‑‑‑‑‑TCCAC‑‑‑‑‑C‑CAGACTACTGTCAGt                                                                                                >  1:2885932/1‑150 (MQ=255)

                                                            cGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTTA‑‑‑‑‑‑TCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAg                                                                                           >  1:3766774/1‑150 (MQ=255)

                                                                gggCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTTA‑‑‑‑‑‑TCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTcc                                                                                       <  1:2593073/150‑1 (MQ=255)

                                                                                  cttccggtaACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTTA‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaa                                                                           >  1:1458525‑M1/5‑107 (MQ=255)

                                                                                   tttCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTTA‑‑‑‑‑‑TCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGTTCCTGCAAGGTGGAGTTCCGTc                                                                    >  1:1492172/1‑150 (MQ=255)

                                                                                       ggTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTA‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccg                                                                      <  1:3035300‑M1/150‑49 (MQ=255)

                                                                                        gTAACGACCTCTTCTGACACTGGCGTTGAATTTTTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTA‑‑‑‑‑‑TCCACTTCCACTCAGACCAGAGTCAGTGCCAGTTCCTGCAAGGTGGAATTCCGt                                                                     <  1:1386464/150‑1 (MQ=255)

                                                                                        gTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTTA‑‑‑‑‑‑TCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGt                                                               >  1:691714/1‑150 (MQ=255)

                                                                                         tAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTA‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtc                                                                    >  1:3446970‑M1/1‑100 (MQ=255)

                                                                                          aacgacctctTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTA‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtca                                                                   <  1:558435‑M1/146‑52 (MQ=255)

                                                                                           acgacctcTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTA‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcac                                                                  >  1:972753‑M1/4‑98 (MQ=255)

                                                                                           acaacCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTTA‑‑‑‑‑‑TCCAC‑‑‑‑‑C‑CAGACTACTGTCAg                                                                                                 >  1:2462667/1‑113 (MQ=255)

                                                                                            cgacctCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTA‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacg                                                                 >  1:3476458‑M1/3‑97 (MQ=255)

                                                                                             gaccTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTTA‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacgg                                                                >  1:3366028‑M1/2‑96 (MQ=255)

                                                                                               ccTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTA‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggta                                                              >  1:2639447‑M1/1‑94 (MQ=255)

                                                                                               ccTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTA‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggta                                                              >  1:922329‑M1/1‑94 (MQ=255)

                                                                                                    tctGACACCGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTTA‑‑‑‑‑‑TCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTg                                                   >  1:874421/1‑150 (MQ=255)

                                                                                                     ctGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTATTTA‑‑‑‑‑‑TCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTgg                                                  <  1:2428182/150‑1 (MQ=255)

                                                                                                       gACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTTA‑‑‑‑‑‑TTCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTGGaa                                                <  1:73219/150‑1 (MQ=255)

                                                                                                       gACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTA‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacg                                                      <  1:2768996‑M1/150‑65 (MQ=255)

                                                                                                       gACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTA‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacg                                                      >  1:1283038‑M1/1‑86 (MQ=255)

                                                                                                       gACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTA‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacg                                                      >  1:2069707‑M1/1‑86 (MQ=255)

                                                                                                        acacTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTTA‑‑‑‑‑‑TCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGATGCTGGaaa                                               <  1:340663/150‑1 (MQ=255)

                                                                                                          acTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTTA‑‑‑‑‑‑TCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTGGAAAAt                                             <  1:2506100/150‑1 (MQ=255)

                                                                                                          acTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTA‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctg                                                   <  1:1577958‑M1/150‑68 (MQ=255)

                                                                                                          acTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTGTAGCCACTTCCAC‑‑‑‑‑G‑CAGACCGGAGTCAGTGACAGTTCCTGCAAGGTGGAATTCCGTCACGGTAACATGACGCTg                                                   <  1:2990752/150‑1 (MQ=255)

                                                                                                            tGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTA‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctgga                                                 <  1:3104333‑M1/150‑70 (MQ=255)

                                                                                                              gCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTA‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaa                                               <  1:3879963‑M1/150‑72 (MQ=255)

                                                                                                              gCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTA‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaa                                               >  1:3737785‑M1/1‑79 (MQ=255)

                                                                                                               cGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTA‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaa                                              <  1:1911723‑M1/150‑73 (MQ=255)

                                                                                                                gTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTA‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaat                                             <  1:839343‑M1/150‑74 (MQ=255)

                                                                                                                  tGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTA‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgaagctggaaaatcc                                           >  1:2110952‑M1/1‑75 (MQ=255)

                                                                                                                   gAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTA‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatcca                                          >  1:3308634‑M1/1‑74 (MQ=255)

                                                                                                                    aaTTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGTGCCCTCTTTA‑‑‑‑‑‑TCCACTTCCACTCAGACCAGAGTCAGTGCCAGTTCCTGCAAAGTGGAATTCCGTCACGGTAACATGACGCTGGAAAATccat                                         >  1:636158/1‑148 (MQ=255)

                                                                                                                     attattACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTACTCTTACTCCGTCTTTA‑‑‑‑‑‑TCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTGGAAAATCCTTCACCaga                                  <  1:296261/150‑1 (MQ=255)

                                                                                                                      ttattaCCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTA‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcg                                       >  1:4177738‑M1/1‑71 (MQ=255)

                                                                                                                       tattaCCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTA‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgc                                      >  1:181849‑M1/1‑70 (MQ=255)

                                                                                                                       tattaCCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTA‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgc                                      <  1:765290‑M1/150‑81 (MQ=255)

                                                                                                                        attaCCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTA‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgcc                                     >  1:1309718‑M1/1‑69 (MQ=255)

                                                                                                                         ttaCCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTA‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgcca                                    >  1:2031820‑M1/1‑68 (MQ=255)

                                                                                                                             ctgtagaaataacgccgGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTA‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagc                                >  1:2927192‑M1/9‑64 (MQ=255)

                                                                                                                              tgtagaaataacgccGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTTA‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagct                               >  1:2396157‑M1/8‑63 (MQ=255)

                                                                                                                                   ggaTAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTTA‑‑‑‑‑‑TCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTGGAAAATCCTTCACCAGAGCTGCTCACGgtgt                    <  1:1183228/149‑1 (MQ=255)

                                                                                                                                   agaTAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTTA‑‑‑‑‑‑TCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTGGAAAATCCTTCACCAGAGCTGCTCACGgtgt                    <  1:1157749/150‑1 (MQ=255)

                                                                                                                                   aaataACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTA‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagaactgctca                          >  1:3068209‑M1/3‑58 (MQ=255)

                                                                                                                                    aatAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTA‑‑‑‑‑‑CCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgcccac                         <  1:4226136‑M1/149‑94 (MQ=255)

                                                                                                                                     aTAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTTA‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgctcacg                        <  1:3668752‑M1/150‑95 (MQ=255)

                                                                                                                                       aaCGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTTA‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgctcacgat                      <  1:3163688‑M1/150‑97 (MQ=255)

                                                                                                                                         cGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTTA‑‑‑‑‑‑TCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTGGAAAATCCTTCACCAGAGCTGCTCACGGTGTTGATcc              <  1:155592/150‑1 (MQ=255)

                                                                                                                                         cGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTTA‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgctcacggtgt                    <  1:1634312‑M1/150‑99 (MQ=255)

                                                                                                                                          gCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTTA‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgctcacggtgtt                   >  1:2554621‑M1/1‑51 (MQ=255)

                                                                                                                                          gCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTA‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgctcacggtgtt                   <  1:1674958‑M1/150‑100 (MQ=255)

                                                                                                                                              gATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTA‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgctcacggtgttgatc               <  1:467685‑M1/150‑104 (MQ=255)

                                                                                                                                              aatGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTA‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgctcacggtgttgatc               <  1:1732936‑M1/149‑104 (MQ=255)

                                                                                                                                               aTGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTA‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgctcacggtgttgatcc              <  1:3275852‑M1/150‑105 (MQ=255)

                                                                                                                                                     cGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTTA‑‑‑‑‑‑TCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTGGAAAATCCTTCACCAGAGCTGCTCACGGTGTTGATCCGCGa          <  1:1082545/142‑1 (MQ=255)

TGCTCGAGAAAATGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGACTTTGGCAAAACGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTTA‑‑‑‑‑‑TCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTGGAAAATCCTTCACCAGAGCTGCTCACGGTGTTGATCCGCGAACTGAGTG  >  Xen14_126/1894‑2192

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`