BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	Xen14_126	2,023	G→A	V79V (GTG→GTA)	Xen14_04726 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Xen14_126	2,023	0	G	A	82.7%	2114.2 / inf	1106	V79V (GTG→GTA)	Xen14_04726	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (78/113); new base A (463/452); total (541/565)
Fisher's exact test for biased strand distribution p-value = 1.68e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1111 total aligned reads displayed.

GTGCTTGTGTGGCCCAGATTGCTCGAGAAAATGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGACTTTGGCAAAACGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTTA‑‑‑‑‑‑TCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTGGAAAATCCTTCACCAGAGCTGCTCACGGT  >  Xen14_126/1875‑2172

       tgttgcacAGATTGCACGTGAAAATGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAAc                                                                                                                                                           >  1:2385985/8‑150 (MQ=255)

        gttgcacAGATTGCACGTGAAAATGGCGTCAATGATAATGTTATTTTCAAATGGCTCGGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGTGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACg                                                                                                                                                          >  1:1918637/7‑150 (MQ=255)

        gttgcacAGATTGCACGTGAAAATGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACg                                                                                                                                                          <  1:3842438/144‑1 (MQ=255)

         ttgcacAGATTGCACGTGAAAATGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGc                                                                                                                                                         >  1:3273942/6‑150 (MQ=255)

          tgcacAGATTGCACGTGAAAATGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAAcacc                                                                                                                                                        >  1:3241847/5‑147 (MQ=255)

           gcacAGATTGCACGTGAAAATGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCg                                                                                                                                                       <  1:2576261/147‑1 (MQ=255)

              cAGATTGCTCGAGAAAATGGCGTCAATGATAATTTTATTTTCAAATGGCTCAGACTTTGGCAAAACGAAGGGCATATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGAt                                                                                                                                                    <  1:4204239/150‑1 (MQ=255)

              cAGATTGCACGTGAAAATGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGAt                                                                                                                                                    >  1:4021803/1‑150 (MQ=255)

                gATTGCACGTGAAAATGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGCGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGa                                                                                                                                                  <  1:2269687/150‑1 (MQ=255)

                 aTTGCACGTGAAAATGGCATCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAg                                                                                                                                                 <  1:3958410/150‑1 (MQ=255)

                  ttgcacgtgaAAATGGCGTCAATGATAATGTTATTTTCAAATTGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGc                                                                                                                                                <  1:2135278/142‑1 (MQ=255)

                   tgcacgtgaAAATGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGTGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGca                                                                                                                                               <  1:2909106/143‑2 (MQ=255)

                   tgcacgtgaAAATGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGca                                                                                                                                               <  1:3912443/143‑2 (MQ=255)

                   tgcacgtgaAAATGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGca                                                                                                                                               <  1:1109179/143‑2 (MQ=255)

                   tgcacgtgaAAATGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGca                                                                                                                                               <  1:2238123/143‑2 (MQ=255)

                     cacgtgaAAATGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGcaga                                                                                                                                             >  1:585925/6‑147 (MQ=255)

                     cacgtgaAAATGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGcaga                                                                                                                                             >  1:494272/6‑147 (MQ=255)

                      acgtgaAAATGGCGTAAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCATGTTTCGCGGCGTCTTCCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGaa                                                                                                                                            <  1:782567/146‑1 (MQ=255)

                       cgtgaAAATGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACGCTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGaaa                                                                                                                                           >  1:1361686/4‑150 (MQ=255)

                       cgtgaAAATGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGaaa                                                                                                                                           >  1:2345267/4‑150 (MQ=255)

                        gtgaAAATGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAg                                                                                                                                          >  1:2063302/3‑150 (MQ=255)

                           aaaaTGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAAc                                                                                                                                       <  1:1425862/150‑1 (MQ=255)

                            aaaTGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAAcc                                                                                                                                      <  1:1221144/150‑1 (MQ=255)

                             aaTGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGACTTTGGCAAAACGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCg                                                                                                                                     >  1:3462000/1‑150 (MQ=255)

                               tGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAAcctgt                                                                                                                                   >  1:3110946/1‑147 (MQ=255)

                                ggCGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCtgtg                                                                                                                                  <  1:119687/150‑1 (MQ=255)

                                   gTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGTGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcg                                                                                                                               >  1:812522/1‑149 (MQ=255)

                                     cAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggc                                                                                                                             >  1:1379286/1‑147 (MQ=255)

                                     cAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggc                                                                                                                             >  1:717588/1‑147 (MQ=255)

                                          aTAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGTGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggccattg                                                                                                                        >  1:1479343/1‑142 (MQ=255)

                                            aaTGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggccattgcg                                                                                                                      <  1:1691183/150‑11 (MQ=255)

                                             aTGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGGACCTGTGgcggccattgcgc                                                                                                                     >  1:3598559/1‑139 (MQ=255)

                                             aTGTTATTTTCAAATGGCTCAGACTTTGGCAAAACGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTc                                                                                                                     >  1:3063117/1‑150 (MQ=255)

                                              tGTTATTTTCAAATGGCTCAGACTTTGGCAAAACGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTcc                                                                                                                    >  1:1159639/1‑150 (MQ=255)

                                               gTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggccattgcgcca                                                                                                                   >  1:154362/1‑137 (MQ=255)

                                               gTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggccattgcgcca                                                                                                                   >  1:1879314/1‑137 (MQ=255)

                                               gTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggccattgcgcca                                                                                                                   >  1:2665582/1‑137 (MQ=255)

                                                ttATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggccattgcgccat                                                                                                                  <  1:876211/150‑15 (MQ=255)

                                                ttATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggccattgcgccat                                                                                                                  <  1:951025/150‑15 (MQ=255)

                                                ttATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggccattgcgccat                                                                                                                  <  1:1592479/150‑15 (MQ=255)

                                                ttATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCAGGATGAGCAGAAAGAACCTGTGgcggccattgcgccac                                                                                                                  >  1:1000253/1‑136 (MQ=255)

                                                  aTTTTCAAATGGCTCAGACTTTGGCAAAACGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAGCCTCTTCTTACACTTGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCt                                                                                                                <  1:4249892/150‑1 (MQ=255)

                                                   ttttCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCATGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCg                                                                                                                                                       >  1:1819162/1‑110 (MQ=255)

                                                   tttgcAAATGGCTCAGGCTCTGGCAGAACGAAGGGCATGTTTCGCGGCGTCTTCCGGTAACAACCTCTGCTTACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggccattg                                                                                                                        <  1:2863967/137‑9 (MQ=255)

                                                      tCAAATGGCTCAGGCTTTGGAAAAACGAAGGGCGTATATCGCGGCTTCTTTCGGTAACAACCTCCTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTTA‑‑‑‑‑‑t                                                                                                      <  1:1941545/150‑1 (MQ=255)

                                                        aaaTGGCTCAGACTTTGGCAAAACGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTTA‑‑‑‑‑‑Tcc                                                                                                    <  1:3180457/150‑1 (MQ=255)

                                                            ggCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTA‑‑‑‑‑‑TCCActt                                                                                                <  1:462192/150‑3 (MQ=255)

                                                             gCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAGAGAACCTGTGGCGGCCATTGCGCCATCTTTA‑‑‑‑‑‑TCCActtc                                                                                               >  1:4147778/1‑147 (MQ=255)

                                                              cTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAGCGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTA‑‑‑‑‑‑TCCActtcc                                                                                              >  1:2713612/1‑146 (MQ=255)

                                                              cTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTA‑‑‑‑‑‑TCCActtcc                                                                                              <  1:1087664/150‑5 (MQ=255)

                                                                caggctctGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTA‑‑‑‑‑‑TCCActtccac                                                                                            <  1:3992358/143‑7 (MQ=255)

                                                                caggctctGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTA‑‑‑‑‑‑TCCActtccac                                                                                            <  1:1623804/143‑7 (MQ=255)

                                                                caggctctGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTA‑‑‑‑‑‑TCCActtccac                                                                                            <  1:2508403/143‑7 (MQ=255)

                                                                caggctctGGCAGAACGAAGGGCATGTTTCGCGGCGTCTTCCGGTAACAACCTCTTCTGACACTGGCGTTGAATTACTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTTA‑‑‑‑‑‑TCCActtccac                                                                                            >  1:553442/8‑144 (MQ=255)

                                                                    ctctGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTA‑‑‑‑‑‑TCCActtccactcag                                                                                        >  1:2450715/4‑140 (MQ=255)

                                                                    ctctGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTA‑‑‑‑‑‑TCCActtccactcag                                                                                        <  1:2196385/147‑11 (MQ=255)

                                                                     tctGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTA‑‑‑‑‑‑TCCActtccactcaga                                                                                       <  1:1721219/148‑12 (MQ=255)

                                                                        ggcagatcGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGa                                                                                                                                         >  1:1035473/8‑103 (MQ=255)

                                                                        ggCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTA‑‑‑‑‑‑TCCActtccactcagacca                                                                                    >  1:1339272/1‑136 (MQ=255)

                                                                          cAAAACGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTTA‑‑‑‑‑‑TCCAC‑‑‑‑‑C‑CAGACTACTGTCAGt                                                                            >  1:330230/1‑150 (MQ=255)

                                                                          cAAAACGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTTA‑‑‑‑‑‑TCCAC‑‑‑‑‑C‑CAGACTACTGTCAGt                                                                            >  1:2415663/1‑150 (MQ=255)

                                                                                          tatCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTTA‑‑‑‑‑‑TCCAC‑‑‑‑‑C‑CAGACTATTGTCAGTGCCAGCTCCTGCAAgg                                                            <  1:4091611/150‑1 (MQ=255)

                                                                                             cgcgGCGTCTTCCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGACCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTTA‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgca                                                               <  1:1997735‑M1/150‑36 (MQ=255)

                                                                                                  cgTCTTCCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTTA‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtg                                                          >  1:2824010‑M1/1‑110 (MQ=255)

                                                                                                   gTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTTA‑‑‑‑‑‑TCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTcc                                                   >  1:847463/1‑150 (MQ=255)

                                                                                                   gTCTTCCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTTA‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtgg                                                         <  1:363010‑M1/150‑42 (MQ=255)

                                                                                                          ggTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTA‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccg                                                  <  1:1048973‑M1/150‑49 (MQ=255)

                                                                                                          ggTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTA‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccg                                                  <  1:3035300‑M1/150‑49 (MQ=255)

                                                                                                          ggTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTA‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccg                                                  <  1:752847‑M1/150‑49 (MQ=255)

                                                                                                           gTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTA‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgt                                                 >  1:3504450‑M1/1‑101 (MQ=255)

                                                                                                            tAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTA‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtc                                                <  1:1268909‑M1/150‑51 (MQ=255)

                                                                                                            tAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACACCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTA‑‑‑‑‑‑TCCACTTCCACTCAGACCAGAGTCAGTGCCAGTTCCTGCAAGGTGGAATTCCGTc                                                >  1:679621/1‑150 (MQ=255)

                                                                                                              acgacctcTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTA‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcac                                              <  1:1306181‑M1/147‑53 (MQ=255)

                                                                                                              acgacctcTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTA‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcac                                              >  1:972753‑M1/4‑98 (MQ=255)

                                                                                                              acaacCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTTA‑‑‑‑‑‑TCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAAc                                        <  1:2767202/150‑1 (MQ=255)

                                                                                                                gaccTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTTA‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacgg                                            >  1:3882844‑M1/2‑96 (MQ=255)

                                                                                                                gaccTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTTA‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacgg                                            >  1:3366028‑M1/2‑96 (MQ=255)

                                                                                                                 acCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTA‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggt                                           <  1:3540668‑M1/150‑56 (MQ=255)

                                                                                                                  ccTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTA‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggta                                          >  1:2639447‑M1/1‑94 (MQ=255)

                                                                                                                   ctctTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTA‑‑‑‑‑‑TCCACttccactcagaccggagtcagtgccagttcctgcaaggtggaattccgtcacggtaa                                         <  1:209444‑M1/150‑58 (MQ=255)

                                                                                                                    tcttctGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTA‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccg                                                  >  1:1453044‑M1/1‑92 (MQ=255)

                                                                                                                     cttctGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTA‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaaca                                       <  1:2319466‑M1/150‑60 (MQ=255)

                                                                                                                     cttctGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTA‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaaca                                       >  1:1966291‑M1/1‑91 (MQ=255)

                                                                                                                      ttctGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTTA‑‑‑‑‑‑TCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCt                                >  1:1793062/1‑150 (MQ=255)

                                                                                                                        ctGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTTA‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatga                                    >  1:1649395‑M1/1‑88 (MQ=255)

                                                                                                                        ctGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTA‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatga                                    >  1:3752930‑M1/1‑88 (MQ=255)

                                                                                                                        ctGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTA‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatga                                    >  1:1519522‑M1/1‑88 (MQ=255)

                                                                                                                             acTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTGTAGCCACTTCCAC‑‑‑‑‑G‑CAGACCGGAGTCAGTGACAGTTCCTGCAAGGTGGAATTCCGTCACGGTAACATGACGCTg                               <  1:2990752/150‑1 (MQ=255)

                                                                                                                              cTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGANAGAACCGGTGGCGGCCATTGCGCCATCTTTA‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctgg                              <  1:3703592‑M1/150‑69 (MQ=255)

                                                                                                                               tGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTTA‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaat                                                      >  1:3389532‑M1/1‑81 (MQ=255)

                                                                                                                                ggCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTTA‑‑‑‑‑‑TCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTACGTCACGGTAACATGACGCTGGAAAATCCt                      <  1:2468464/150‑1 (MQ=255)

                                                                                                                                 gCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTTA‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaa                           >  1:3437966‑M1/1‑79 (MQ=255)

                                                                                                                                  cGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTA‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaa                          >  1:3798466‑M1/1‑78 (MQ=255)

                                                                                                                                      gAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTA‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatcca                      >  1:1753404‑M1/1‑74 (MQ=255)

                                                                                                                                      gAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTA‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatcca                      >  1:1135968‑M1/1‑74 (MQ=255)

                                                                                                                                       aaTTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTA‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccat                     >  1:3577950‑M1/1‑73 (MQ=255)

                                                                                                                                        attattACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTACTCTTACTCCGTCTTTA‑‑‑‑‑‑TCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTGGAAAATCCTTCACCaga              <  1:296261/150‑1 (MQ=255)

                                                                                                                                            ttaCCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTA‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgcca                >  1:2031820‑M1/1‑68 (MQ=255)

                                                                                                                                               ccTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCGTCTTTA‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagag             <  1:621418‑M1/150‑86 (MQ=255)

                                                                                                                                               ccTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCATTGCGCCATCTTTA‑‑‑‑‑‑TCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagag             <  1:2594992‑M1/150‑86 (MQ=255)

GTGCTTGTGTGGCCCAGATTGCTCGAGAAAATGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGACTTTGGCAAAACGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTCTTACTCCGTCTTTA‑‑‑‑‑‑TCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTGGAAAATCCTTCACCAGAGCTGCTCACGGT  >  Xen14_126/1875‑2172

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`