BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	Xen14_106	3,879	(T)_10→11	coding (315/357 nt)	Xen14_04646 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Xen14_106	3,879	1	.	T	95.7%	255.8 / 5.0	94	coding (315/357 nt)	Xen14_04646	hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (2/2); new base T (47/43); total (49/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.70e-01

Only 100 of 103 total aligned reads displayed.

ACTATATCATTATAATCATTAAAAAAGGATAACAAATACAATATTTTAAATATTTTTTTGTTGTTACCTGAGTATTTTATGTCTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATATTTTTTTTTT‑‑ACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGATTACTATTATCGATTCCGACAATTTCCATGTCCCAATGAGTGTTTTTTGCTAAAAAAAACAAGGAGTTGGA  >  Xen14_106/3731‑4028

aCTATATCATTATAATCATTAAAAAAGGATAACAAATACAATATTTTAAATATTTTCTTGTTGTTACCTGAGTATTTTATGTCTGTAATTATATTGGAAGCTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATATTTTTTTTTT‑‑a                                                                                                                                                      >  1:2686783/1‑150 (MQ=255)

    tatCATTATAATCATTAAAAAAGGATAACAAATACAATATTTTAAATATTTTTTTGTTGTTACCTGAGTATTTTATGTCTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATAtttttttttttacat                                                                                                                                                    >  1:1261193/1‑145 (MQ=255)

      tCATTATAATCATTAAAAAAGGATAACAAATACAATATTTTAAATATTTTTTTGTTGTTACCTGAGTATTTTATGTCTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATATTTTTTTTTTT‑ACATaa                                                                                                                                                 <  1:305540/150‑1 (MQ=255)

      tCATTATAATCATTAAAAAAGGATAACAAATACAATATTTTAAATATTTTTTTGTTGTTACCTGAGTATTTTATGTCTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATATTTTTTTTTTT‑ACATaa                                                                                                                                                 >  1:3527106/1‑150 (MQ=255)

      tCATTATAATCATTAAAAAAGGATAACAAATACAATATTTTAAATATTTTTTTGTTGTTACCTGAGTATTTTATGTCTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATATTTTTTTTTT‑‑ACATAAc                                                                                                                                                <  1:924861/150‑1 (MQ=255)

       cATTATAATCATTAAAAAAGGATAACAAATACAATATTTTAAATATTTTTTTGTTGTTACCTGAGTATTTTATGTCTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATATTTTTTTTTTT‑ACATAAc                                                                                                                                                <  1:1094953/150‑1 (MQ=255)

       cATTATAATCATTAAAAAAGGATAACAAATACAATATTTTAAATATTTTTTTGTTGTTACCTGAGTATTTTATGTCTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATATTTTTTTTTTT‑ACATAAc                                                                                                                                                <  1:1094949/150‑1 (MQ=255)

       cATTATAATCATTAAAAAAGGATAACAAATACAATATTTTAAATATTTTTTTGTTGTTACCTGAGTATTTTATGTCTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATATTTTTTTTTTT‑ACATAAc                                                                                                                                                <  1:1094952/150‑1 (MQ=255)

       cATTATAATCATTAAAAAAGGATAACAAATACAATATTTTAAATATTTTTTTGTTGTTACCTGAGTATTTTATGTCTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATATTTTTTTTTTT‑ACATAAc                                                                                                                                                <  1:1094950/150‑1 (MQ=255)

        aTTATAATCATTAAAAAAGGATAACAAATACAATATTTTAAATATTTTTTTGTTGTTACCTGAGTATTTTATGTCTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAAtttttttttttttgcataaca                                                                                                                                                >  1:4216091/1‑141 (MQ=255)

        aTTATAATCATTAAAAAAGGATAACAAATACAATATTTTAAATATTTTTTTGTTGTTACCTGAGTATTTTATGTCTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATATTTTTTTTTTT‑ACATAACa                                                                                                                                               >  1:1017466/1‑150 (MQ=255)

        aTTATAATCATTAAAAAAGGATAACAAATACAATATTTTAAATATTTTTTTGTTGTTACCTGAGTATTTTATGTCTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATATTTTTTTTTTT‑ACATAACa                                                                                                                                               >  1:1695882/1‑150 (MQ=255)

         ttATAATCATTAAAAAAGGATAACAAATACAATATTTTAAATATTTTTTTGTTGTTACCTGAGTATTTTATGTCTGTAATTATATCGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATATTTTTTTTTTT‑ACATAACaa                                                                                                                                              <  1:5427/150‑1 (MQ=255)

          tataATCATTAAAAAAGGATAACAAACACAATATTTTAAATATTTTTTTGTTGTTACCTGAGTATTTTATGTCTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATATTTTTTTTTTT‑ACATAACAag                                                                                                                                             >  1:275220/1‑150 (MQ=255)

              aTCATTAAAACAGGATAACAAATACAATATTTTAAATATTTTTTTGTTGTTACATGAGTATTTTATGTCTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATATTTTTTTTTTT‑ACATAACAAGAGtc                                                                                                                                         >  1:1952408/1‑150 (MQ=255)

                cATTAAAAAAGGATAACAAATACAATATTTTAAATATTTTTTTGTTGTTACCTGAGTATTTTATGTCTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATATTTTTTTTTTTTACATAACAAGAGtca                                                                                                                                        >  1:4116311/1‑150 (MQ=255)

                cATTAAAAAAGGATAACAAATACAATATTTTAAATATTTTTTTGTTGTTACCTGAGTATTTTATGTCTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATATTTTTTTTTTT‑ACATAACAAGAGtcat                                                                                                                                       >  1:1258063/1‑150 (MQ=255)

                cATTAAAAAAGGATAACAAATACAATATTTTAAATATTTTTTTGTTGTTACCTGAGTATTTTATGTCTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATATTTTTTTTTTT‑ACATAACAAGAGtcat                                                                                                                                       >  1:3391707/1‑150 (MQ=255)

                    aaaaaaGGATAACAAATACAATATTTTCAATATTTTTTTGTTGTTACCTGAGTATTTTATGTCTGTCATTCTATTGGAAACCATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATATTTTTTTTTTT‑ACCTACCAAGAGTCATCAga                                                                                                                                   >  1:1196643/1‑150 (MQ=255)

                     aaaaaGGATAACAAATACAATATTTTAAATATTTTTTTGTTGTTACCTGAGTATTTTATGTCTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATATTTTTTTTTTT‑ACATAACAAGAGtcatc                                                                                                                                      <  1:1050240/146‑1 (MQ=255)

                     aaaaaGGATAACAAATACAATATTTTAAATATTTTTTTGTTGTTACCTGAGTATTTTATGTCTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATATTTTTTTTTTT‑ACATAACAAGAGtcatc                                                                                                                                      >  1:34599/1‑146 (MQ=255)

                     aaaaaGGATAACAAATACAATATTTTAAATATTTTTTTGTTGTTACCTGAGTATTTTATGTCTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATATTTTTTTTTTT‑ACATAACAAGAGTCATCAgat                                                                                                                                  <  1:1657786/150‑1 (MQ=255)

                      aaaaGGATAACAAATACAATATTTCAAATATTTTTTTGTTGTTACCTGAGTATTTTATGTCTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATATTTTTTTTTTT‑ACATAACAAGAGTCATCAgatg                                                                                                                                 >  1:2459605/1‑150 (MQ=255)

                         aGGATAACAAATACAATATTTTAAATATTTTTTTGTTGTTACCTGAGTATTTTATGTCTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATATTTTTTTTTTT‑ACATAACAAGAGTCATCAGATGata                                                                                                                              >  1:4255944/1‑150 (MQ=255)

                         aGGATAACAAATACAATATGTTAAATATTTTTTTGTTGGTACCTGAGTATTTTATGTCTGTAATTATATTGGAAACTGTATTCAAG‑GCGTTTCATGGAGGTTCGCAGTAAATATTTTTTTTGT‑‑ACATAACAAGAGTCATCAGATGATAtt                                                                                                                            <  1:3980613/150‑1 (MQ=255)

                            aTAACAAATACAATATTTTAAATATTTTTTTGTTGTTACCTGAGTATTTTATGTCTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATATTTTTTTTTTT‑ACATAACAAGAGTCATCAGATGATATTg                                                                                                                           >  1:551064/1‑150 (MQ=255)

                            aTAACAAATACAATATTTTAAATATTTTTTTGTTGTTACCTGAGTATTTTATGTCTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATATTTTTTTTTTT‑ACATAACAAGAGTCATCAGATGATATTg                                                                                                                           <  1:4007674/150‑1 (MQ=255)

                            aTAACAAATACAATATTTTAAATATTTTTTTGTTGTTACCTGAGTATTTTATGTCTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATATTTTTTTTTTT‑ACATAACAAGAGTCATCAGATGATATTg                                                                                                                           >  1:551073/1‑150 (MQ=255)

                            aTAACAAATACAATATTTTAAATATTTTTTTGTTGTTACCTGAGTATTTTATGTCTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATATTTTTTTTTTT‑ACATAACAAGAGTCATCAGATGATATTg                                                                                                                           >  1:551063/1‑150 (MQ=255)

                            aTAACAAATACAATATTTTAAATATTTTTTTGTTGTTACCTGAGTATTTTATGTCTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGATTCGCAGTAAATATTTTTTTTTTT‑ACATAACAAGAGTCATCAGATGATATTg                                                                                                                           <  1:1267381/150‑1 (MQ=255)

                             tAACAAATACAATATTTTAAATATTTTTTTGTTGTTACCTGAGTATTTTATGTCTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATATTTTTTTTTTTTACATAACAAGAGTCATCAGATGATATTg                                                                                                                           <  1:4045335/150‑1 (MQ=255)

                               aCAAATACAATATTTTAAATATTTTTTTGTTGTTACCTGAGTATTTTATGTCTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATATTTTTTTTTTT‑ACATAACAAGAGTCATCAGATGATATTGAAt                                                                                                                        >  1:45172/1‑150 (MQ=255)

                               aCAAATACAATATTTTAAATATTTTTTTGTTGTTACCTGAGTATTTTATGTCTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATATTTTTTTTTTT‑ACATAACAAGAGTCATCAGATGATATTGAAt                                                                                                                        >  1:45173/1‑150 (MQ=255)

                                cAAATACAATCTTTTAAATATTTTTTTGTTGTTACCTGAGTATTTTATGTCTGTGATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGGAAATATTTTTTTTTTTTACATAACAAGAGTCATCAGATGATATTGAAt                                                                                                                        <  1:3711114/150‑1 (MQ=255)

                                cAAATACAATATTTTAAATATTTGTTTGTTGTTACCTGAGTATTTTATGTCTTTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATATTTTTTTTTTT‑ACATAACAAGAGTCATCAGATGATATTGAATg                                                                                                                       <  1:4107808/150‑1 (MQ=255)

                                   aTACAATATTTTAAATGTTTTTTTTTTGTTACCTGAGTGTTTTATGTCTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATATTTTTTTTTTT‑ACATAACAAGAGTCATCAGATGATATTGAATgcag                                                                                                                    <  1:3175245/150‑1 (MQ=255)

                                   aTACAATATTTTAAATATTTTTTTGTTGTTACCTGAGTATTTTATGTCTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATATTTTTTTTTTT‑ACATAACAAGAGTCATCAGATGATATTGAATgcag                                                                                                                    <  1:3926622/150‑1 (MQ=255)

                                   aTACAATATTTTAAATATTTTTTTGTTGTTACCTGAATATTTTATGTCTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATATTTTTTTTTTT‑ACATAACAAGAGTCATCAGATGATATTGAATgcag                                                                                                                    >  1:531699/1‑150 (MQ=255)

                                             ttAAATATTTTTTTGTTGTTACCTGAGTATTTTATGTCTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATATTTTTTTTTTT‑ACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACttaatt                                                                                                          <  1:790818/150‑1 (MQ=255)

                                             ttAAATATTTTTTTGTTGTTACCTGAGTATTTTATGTCTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATATTTTTTTTTTT‑ACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACttaatt                                                                                                          <  1:790814/150‑1 (MQ=255)

                                                 atatTTTTTTGTTGTTACCTGAGTATTTTATGTCTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATATTTTTTTTTTT‑ACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATg                                                                                                      >  1:2066014/1‑150 (MQ=255)

                                                  tatTTTTTTGTTGTTACCTGAGTATTTTATGTCTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATATTTTTTTTTTT‑ACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGc                                                                                                     >  1:1789939/1‑150 (MQ=255)

                                                       ttttGTTGTTACCTGAGTATTTTATGTCTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATATTTTTTTTTTT‑ACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGcatcat                                                                                                >  1:4027287/1‑150 (MQ=255)

                                                          tgttgtTACCTGAGTATTTTATGTCTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATATTTTTTTTTTT‑ACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCaa                                                                                             <  1:2589757/150‑1 (MQ=255)

                                                          tgttgtTACCTGAGTATTTTATGTCTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATATTTTTTTTTTT‑ACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCaa                                                                                             <  1:1233665/150‑1 (MQ=255)

                                                          tgttgtTACCTGAGTATTTTATGTCTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATATTTTTTTTTTT‑ACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCaa                                                                                             <  1:2589756/150‑1 (MQ=255)

                                                          tgttgtTACCTGAGTATTTTATGTCTGTAATTATATTGGAAACTACATTCAATTGCATTTCATTGAGGTTCGCAGTAAATATTTTTTTTTTTTACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCAtcatca                                                                                              <  1:1238732/150‑1 (MQ=255)

                                                              gttACCTGAGTATTTTATGTCTGTAATTATATTGTAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATATTTTTTTTTTT‑ACATAACAAGAGTCATAAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATtat                                                                                         >  1:3694272/1‑150 (MQ=255)

                                                              gttACCTGAGTATTTTATGTCTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATATTTTTTTTTTT‑ACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGcatca                                                                                                 <  1:869954/142‑1 (MQ=255)

                                                                tACCTGAGTATTTTATGTCTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATATTTTTTTTTTT‑ACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATaa                                                                                       >  1:2913741/1‑150 (MQ=255)

                                                                 aCCTGAGTATTTTATGTCTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATATTTTTTTTTTT‑ACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAAt                                                                                      >  1:2506519/1‑150 (MQ=255)

                                                                    tGAGTATTTTATGTCTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATATTTTTTTTTTT‑ACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAAtttt                                                                                   >  1:650043/1‑150 (MQ=255)

                                                                     gAGTATTTTATGTCTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATATTTTTTTTTTT‑ACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAAttttt                                                                                  <  1:894228/150‑1 (MQ=255)

                                                                           tttATGTCTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATATTTTTTTTTT‑‑ACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTg                                                                               >  1:3421198/1‑146 (MQ=255)

                                                                            ttATGTCTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATATTTTTTTTTTT‑ACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACg                                                                           <  1:1569787/150‑1 (MQ=255)

                                                                               tGTCTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATATTTTTTTTTTT‑ACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGAt                                                                        >  1:3594715/1‑150 (MQ=255)

                                                                               tGTCTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATATTTTTTTTTTT‑ACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGAt                                                                        >  1:356119/1‑150 (MQ=255)

                                                                                  cTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCGGTAAATATTTTTTTTTTT‑ACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGATTAc                                                                     >  1:305503/1‑150 (MQ=255)

                                                                                  cTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATATTTTTTTTTTT‑ACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGATTAc                                                                     >  1:2644217/1‑150 (MQ=255)

                                                                                  cTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATATTTTTTTTTTT‑ACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGATTAc                                                                     >  1:3805133/1‑150 (MQ=255)

                                                                                    gTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATATTTTTTTTTTT‑ACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGATTACta                                                                   <  1:3322928/150‑1 (MQ=255)

                                                                                     tAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATATTTTTTTTTTT‑ACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGATTACtat                                                                  >  1:37532/1‑150 (MQ=255)

                                                                                       aTTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATATTTTTTTTTTT‑ACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGATTACtatta                                                                >  1:1899293/1‑150 (MQ=255)

                                                                                         tataTTGGAAACTATATTCAATTGCACTTCATTGAGGTTCGCAGTAAATATTTTTTTTTTT‑ACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGATTACTATTATc                                                              <  1:1622551/150‑1 (MQ=255)

                                                                                            atTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATATTTTTTTTTTT‑ACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGATTACTATTATCGAt                                                           >  1:417546/1‑150 (MQ=255)

                                                                                               ggAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATATTTTTTTTTTT‑ACATAACAAGAGTCATCAGATGATGTTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGATTACTATTATCGATTcc                                                        <  1:1736161/150‑1 (MQ=255)

                                                                                                    cTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATATTTTTTTTTTT‑ACATAACAAGAGTCATCAGATGACATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGATTACTATTATCGATTCCGACaa                                                   >  1:1750303/1‑150 (MQ=255)

                                                                                                        atTCAATTGCATTTCATTGAGGTTCGCAGTAAATATTTTTTTTTTT‑ACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGATTACTATTATCGATTCCGACAATTTc                                               >  1:2771996/1‑150 (MQ=255)

                                                                                                              ttGCATTTCATTGAGGTTCGCAGTAAATATTTTTTTTTTT‑ACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGATTACTATTATCGATTCCGACAATTTCCATGTc                                         <  1:2146418/150‑1 (MQ=255)

                                                                                                               tGCATTTCATTGAGGTTCGCAGTAAATATTTTTTTTTTT‑ACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGATTACTATTATCGATTCCGACAATTTCCATGTcc                                        >  1:1281516/1‑150 (MQ=255)

                                                                                                               tGCATTTCATTGAGGTTCGCAGTAAATATTTTTTTTTTT‑ACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGATTACTATTATCGATTCCGACAATTTCCATGTcc                                        >  1:1281520/1‑150 (MQ=255)

                                                                                                               tGCATTTCATTGAGGTTCGCAGTAAATATTTTTTTTTTT‑ACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGATTACTATTATCGATTCCGAAAATTTCCATGTcc                                        >  1:1281515/1‑150 (MQ=255)

                                                                                                                gCATTTCATTGAGGTTCGCAGTAAATATTTTTTTTTTT‑ACGTAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGATTACTATTATCGATTCCGATAATTTCCATGTccc                                       <  1:3581782/150‑1 (MQ=255)

                                                                                                                  aTTTCATTGAGGTTCGCAGTAAATATTTTTTTTTTT‑ACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGATTACTATTATCGATTCCGACAATTTACATGTCCCaa                                     >  1:4073868/1‑150 (MQ=255)

                                                                                                                   tttCATTGAGGTTCGCAGTAAATATTTTTTTTTTT‑ACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGATTACTATTATCGATTCCGACAATTTCCATGTCCCAAt                                    <  1:3127618/150‑1 (MQ=255)

                                                                                                                    ttCATTGAGGTTCGCAGTAAATATTTTTTTTTTT‑ACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGATTACTATTATCGATTCCGACAATTTCCATGTCCCAATg                                   <  1:4003874/150‑1 (MQ=255)

                                                                                                                        ttGAGGTTCGCAGTAAATATTTTTTTTTTT‑ACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGATTACTATTATCGATTCCGACAATTTCCATGTCCCAATGAgtg                               <  1:1486516/150‑1 (MQ=255)

                                                                                                                            ggTTCGCAGTAAATATTTTTTTTTTT‑ACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGATTACTATTATCGATTCCGACAATTTCCATGTCCCAATGAGTGtttt                           >  1:164437/1‑150 (MQ=255)

                                                                                                                            ggTTCGCAGTAAATATTTTTTTTTTT‑ACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGATTACTATTATCGATTCCGACAATTTCCATGTCCCAATGAGTGtttt                           >  1:164438/1‑150 (MQ=255)

                                                                                                                             gTTCGCAGTAAATATTTTTTTTTTT‑ACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGATTACTATTATCGATTCCGACAATTTCCATGTCCCAATGAGTGttttt                          <  1:2679603/150‑1 (MQ=255)

                                                                                                                              ttCGCAGTAAATATTTTTTTTTTT‑ACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTATGGATTACTATTATCGATTCCGACAATTTCCATGTCCCAATGAGTGtttttt                         <  1:567585/150‑1 (MQ=255)

                                                                                                                              ttCGCAGTAAATATTTTTTTTTTT‑ACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGATTACTATTATCGATTCCGACAATTTCCATGTCCCAATGAGTGtttttt                         <  1:1026787/150‑1 (MQ=255)

                                                                                                                               tCGCAGTAAATATTTTTTTTTTTTACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGATTACtatta                                                                >  1:649019/1‑111 (MQ=255)

                                                                                                                               tCGCAGTAAATATTTTTTTTTTTTACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGATTACtatta                                                                >  1:649017/1‑111 (MQ=255)

                                                                                                                               tCGCAGTAAATATTTTTTTTTTTTACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGATTACtatta                                                                >  1:649015/1‑111 (MQ=255)

                                                                                                                               tCGCAGTAAATATTTTTTTTTTTTACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGATTACtatta                                                                >  1:649016/1‑111 (MQ=255)

                                                                                                                               tCGCAGTAAATATTTTTTTTTTTTACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGATTACTATTATCGATTCCGACAATTTCCATGTCCCAATGAGTGtttttt                         <  1:3058341/150‑1 (MQ=255)

                                                                                                                               tCGCAGTAAATATTTTTTTTTTT‑ACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGATTACTATTATCGATTCCGACAATTTCCATGTCCCAATGAGTGTTTTTTg                        <  1:3095996/150‑1 (MQ=255)

                                                                                                                               tCGCAGTAAATATTTTTTTTTTT‑ACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGATTACTATTATCGATTCCGACAATTTCCATGTCCCAATGAGTGTTTTTTg                        <  1:1907769/150‑1 (MQ=255)

                                                                                                                               tCGCAGTAAATATTTTTTTTTTT‑ACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATACATCATCAATTATAATTTTTCTGTACGGATTACTATTATCGATTCCGATAATTTCCATGTCCCAATGAGTGTTTTTTg                        >  1:348813/1‑150 (MQ=255)

                                                                                                                                cGCAGTAAATATTTTTTTTTTTTACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGATTACTATTATCGATTCCGACAATTTCCATGTCCCAATGAGTGTTTTTTg                        <  1:1196572/150‑1 (MQ=255)

                                                                                                                                cGCAGTAAATATTTTTTTTTTT‑ACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGATTACTATTATCGATTCCGACAATTTCCATGTCCCAATGAGTGTTTTTTGc                       <  1:3242349/150‑1 (MQ=255)

                                                                                                                                  cAGTAAATATTTTTTTTTTT‑ACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGATTACTATTATCGATTCCGACAATTTCCATGTCCCAATGAGTGTTTTTTGCTa                     <  1:4121415/150‑1 (MQ=255)

                                                                                                                                  cAGTAAATATTTTTTTTTT‑‑ACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGATTACTATTATCGATTCCGACAATTTCCATGTCCCAATGAGTGTTTTTTGCTaa                    >  1:565902/1‑150 (MQ=255)

                                                                                                                                   aGTAAATATTTTTTTTTTT‑ACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGATTACTATTATCGATTCCGACAATTTCCATGTCCCAATGAGTGTTTTTTGCTaa                    >  1:2831554/1‑150 (MQ=255)

                                                                                                                                      aatattttttttttt‑‑ACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGATTACTATTATCGATTCCGACAATTTCCATGTCCCAATGAGTGTTTTTTGCTaaaaaa                <  1:3797952/145‑1 (MQ=255)

                                                                                                                                       atattttttttttt‑‑ACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGATTACTATTATCGATTCCGACAATTTCCATGTCCCAATGAGTGTTTTTTGCTaaaaaaa               <  1:2609299/146‑1 (MQ=255)

                                                                                                                                                  ttt‑‑ACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACNTAATTAATGCATCATCAATTATAATTTTTCTGTA‑GGATTACTATTATCGATTCCGACAATTTCCATGTCCCAATGAGTGTTTTTTGCTAAAAAAAACAAGGAGTTgg   >  1:2043252/1‑150 (MQ=255)

                                                                                                                                                    t‑‑ACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGATTACTATTATCGATTCCGACAATTTCTATGTCCCAATGAGTGTTTTTTGCTAAAAAAAACAAGGCGTTGGa  >  1:3979924/1‑150 (MQ=255)

                                                                                                                                                    t‑‑ACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGATTACTATTATCGATTCCGACAATTTCCATGTCCCAATGAGTGTTTTTTGCTAAAAAAAACAAGGAGTTGGa  >  1:3569653/1‑150 (MQ=255)

ACTATATCATTATAATCATTAAAAAAGGATAACAAATACAATATTTTAAATATTTTTTTGTTGTTACCTGAGTATTTTATGTCTGTAATTATATTGGAAACTATATTCAATTGCATTTCATTGAGGTTCGCAGTAAATATTTTTTTTTT‑‑ACATAACAAGAGTCATCAGATGATATTGAATGCAGCAACTTAATTAATGCATCATCAATTATAATTTTTCTGTACGGATTACTATTATCGATTCCGACAATTTCCATGTCCCAATGAGTGTTTTTTGCTAAAAAAAACAAGGAGTTGGA  >  Xen14_106/3731‑4028

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`