BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	Xen14_191	226	2 bp→GA	intergenic (–/–)	– / –	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Xen14_191	226	0	A	G	88.9%	1381.3 / 112.8	672	intergenic (–/–)	–/–	–/–
Reads supporting (aligned to +/- strand): ref base A (34/40); new base G (278/317); total (314/358)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01
*	Xen14_191	227	0	G	A	89.1%	1383.0 / 108.7	671	intergenic (–/–)	–/–	–/–
Reads supporting (aligned to +/- strand): ref base G (34/39); new base A (279/318); total (314/357)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 673 total aligned reads displayed.

TGATTGTATGCCTGCGATCAATGCCAAACGTGTTTACCGGATCATGAACCGCCCCGGGAATCCTGGAGACTAAACTTCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAACGGGCAGTCCGTATGGTTCTGGAAAGTCAGAGCGAATATGACTCACAATGGGCGACAATTTGTTCCATTGCTCCAAAGATTGGCTGTACGCCGGAGACTCTGCGTGTCTGGGTTCGCCAGCATGAGCGGGATACCGGGGGCGGTGATGGAGGGCTC  >  Xen14_191/84‑376

||

     gTATGCCTGCGATCAATGCCAAACGTGTTTACCGGATCATGAACTGTCCCGGGAATCCTGGAGACTAAACTTCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAACGGGCAGTCCGTATGGtt                                                                                                                                            >  1:2492326/1‑150 (MQ=255)

             gCGATCAATGCCAAACGTGTTTACCGGATCATGAACCGCCCCGGGAATCCTGGAGACTAAACTTCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAACGGGCAGTCCGTATGGTTCTGGAAAg                                                                                                                                    <  1:1633943/150‑1 (MQ=255)

                             gtgtaactgagttaaTGAACCGCCCCGGGAATCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGAc                                                                                                                    <  1:4106994/136‑1 (MQ=14)

                             gtgtTTACCGGATCATGAACCGCCCCGGGAATCCTGGAGACTAAACTTCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAACGGGCAGTCCGTATGGTTCTGGAAAGTCAGAGCGAATATGAc                                                                                                                    <  1:1495489/150‑1 (MQ=255)

                              agtttttccagtattGAACCGCCCCGGGAATCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACt                                                                                                                   >  1:2513991/15‑150 (MQ=14)

                                   caatctgcttGAACCGCCCCGGGAATCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTcacag                                                                                                              <  1:1903713/141‑2 (MQ=17)

                                    ttatgacctGAACCGCCCCGGGAATCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTcacagt                                                                                                             >  1:81378/9‑148 (MQ=17)

                                     ggaaccgtGAACCGCCCCGGGAATCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTcacagtg                                                                                                            >  1:1434957/8‑147 (MQ=17)

                                     ccagtattGAACCGCCCCGGGAATCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTcacagtg                                                                                                            >  1:866754/8‑147 (MQ=17)

                                      gaaccgtGAACCGCCCCGGGAATCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGTGCGAATATGACTCACAGTgg                                                                                                           <  1:1282127/144‑1 (MQ=17)

                                       gATCATGAACCGCCCCGGGNATCCTGGAGACTAAACTTCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAACGGGCAGTCCATATGGTTCTGGAAAGTCAGAGCGAATATGACTCACAATggg                                                                                                          >  1:3126197/1‑150 (MQ=255)

                                        gacctGAACCGCCCCGGGAATCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGc                                                                                                         >  1:3609824/5‑150 (MQ=17)

                                         aactGAACCGCCCCGGGAATCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCg                                                                                                        <  1:1086630/147‑1 (MQ=18)

                                         aactGAACCGCCCCGGGAATCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCg                                                                                                        <  1:2651342/147‑1 (MQ=18)

                                           ttGAACCGCCCCGGGAATCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCggc                                                                                                      >  1:1796610/2‑148 (MQ=18)

                                           gtGAACCGCCCCGGGAATCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCggc                                                                                                      <  1:4479704/149‑3 (MQ=18)

                                           ctGAACCGCCCCGGGAATCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCggc                                                                                                      <  1:1971842/149‑3 (MQ=18)

                                            tGAACCGCCCCGGGAATCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCggca                                                                                                     >  1:1943355/1‑147 (MQ=18)

                                             gAACCGCCCCGGGAATCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATGTGACTCACAGTGGGCGGCaa                                                                                                    >  1:1852474/1‑150 (MQ=17)

                                             gAACCGCCCCGGGAATCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCaa                                                                                                    >  1:1217148/1‑150 (MQ=18)

                                             gAACCGCCCCGGGAATCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCaa                                                                                                    >  1:1022692/1‑150 (MQ=18)

                                              aaCCGCCCCGGGAATCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAAt                                                                                                   <  1:2746039/150‑1 (MQ=18)

                                                  gCCCCGGGAATCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGt                                                                                               <  1:1516553/150‑1 (MQ=18)

                                                  gCCCCGGGAATCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGt                                                                                               >  1:502797/1‑150 (MQ=18)

                                                  gCCCCGGGAATCCTGGAGACTAAACTCCCTGAGAAAGAGATAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGt                                                                                               <  1:3823028/150‑1 (MQ=18)

                                                   ccccGGGAATCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTATAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGtt                                                                                              <  1:3228846/150‑1 (MQ=18)

                                                   ccccGGGAATCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCTATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGtt                                                                                              <  1:3053691/150‑1 (MQ=18)

                                                    cccGGGAATCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTc                                                                                             >  1:9704/1‑150 (MQ=18)

                                                      cGGGAATCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCa                                                                                           >  1:3779045/1‑150 (MQ=21)

                                                        ggAATCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTTCCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCAt                                                                                          >  1:3494042/1‑150 (MQ=18)

                                                         gAATCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTg                                                                                        <  1:3247843/150‑1 (MQ=21)

                                                          aaTCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGc                                                                                       <  1:733920/150‑1 (MQ=21)

                                                            tCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGccc                                                                                     <  1:3310820/150‑3 (MQ=21)

                                                            tCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGccc                                                                                     <  1:63951/150‑3 (MQ=21)

                                                             ccTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGcccc                                                                                    <  1:3110258/150‑4 (MQ=21)

                                                              cTGGAGACTAAACTCCCTGAGAAAGAGGCAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTTAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCa                                                                                   >  1:1645079/1‑150 (MQ=21)

                                                               tGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCaa                                                                                  <  1:557290/150‑1 (MQ=21)

                                                                ggAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCaaa                                                                                 <  1:3513297/150‑1 (MQ=21)

                                                                   gaCTAAACTCCCTGAGAAAGAGGTAAATAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGAt                                                                              <  1:1330023/150‑1 (MQ=21)

                                                                   gaCTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTTTGGAAAATCAGGGAGAATATGACTAACAATGGGTGGCAATTTTTTccctttccccaaaaat                                                                              >  1:4041380/1‑136 (MQ=12)

                                                                    aCTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGAtt                                                                             <  1:365788/150‑1 (MQ=25)

                                                                     gtAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCTTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTg                                                                            <  1:4187605/149‑1 (MQ=21)

                                                                     cTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTg                                                                            >  1:4069228/1‑150 (MQ=25)

                                                                     cTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTg                                                                            >  1:4011996/1‑150 (MQ=25)

                                                                     cTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTg                                                                            <  1:3282607/150‑1 (MQ=25)

                                                                       aaaCTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCTTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGc                                                                          <  1:2798765/150‑1 (MQ=21)

                                                                       aaaCTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGc                                                                          <  1:703075/150‑1 (MQ=25)

                                                                       aaaCTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTC‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGc                                                                          >  1:1048655/1‑150 (MQ=25)

                                                                        aaCTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCt                                                                         <  1:3716201/150‑1 (MQ=25)

                                                                             cccgagaAAGAGGTAAACAGGATGACTAAAAATACTCTTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGcc                                                                                      <  1:2681790/129‑2 (MQ=25)

                                                                              cTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTAcac                                                                   >  1:2361532/1‑148 (MQ=37)

                                                                                 agaAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCgg                                                                <  1:2126132/150‑1 (MQ=37)

                                                                                  gaAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAACGGGCAGTCCGTATGGTTCTGGAAAGTCAGAGCGAATATGACTCACAATGGGCGACAATTTGTTCCATTGCTCCAAAGATTGGCTGTACGCCGga                                                               >  1:2966257/1‑150 (MQ=255)

                                                                                   aaaGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGgag                                                              <  1:2444756/150‑1 (MQ=37)

                                                                                   aaaGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGgag                                                              <  1:1243958/150‑1 (MQ=37)

                                                                                    aaGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGgaga                                                             >  1:3369840/1‑150 (MQ=37)

                                                                                    aaGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGgaga                                                             <  1:946351/150‑1 (MQ=37)

                                                                                      gagGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGAct                                                           >  1:3174584/1‑150 (MQ=255)

                                                                                      gagGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGAct                                                           <  1:3883078/150‑1 (MQ=255)

                                                                                       agGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGActc                                                          <  1:2910326/150‑1 (MQ=255)

                                                                                        ggTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGActct                                                         >  1:3543201/1‑150 (MQ=255)

                                                                                         gTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTg                                                        >  1:886537/1‑150 (MQ=255)

                                                                                           aaaCAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAACGGGCAGTCCGTATGGTTCTGGAAAGTCAGAGCGAATATGACTCACAATGGGCGACAATTTGTTCCATTGCTCCAAAGATTGGCTGTACGCCGGAGACTCTGCg                                                      >  1:3339657/1‑150 (MQ=255)

                                                                                           aaaCAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAACGGGCAGTCCGTATGGTTCTGGAAAGTCAGAGCGAATATGACTCACAATGGGCGACAATTTGTTCCATTGCTCCAAAGATTGGCTGTACGCCGGAGACTCTGCg                                                      >  1:2061644/1‑150 (MQ=255)

                                                                                             acaagATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACATTGGGCGGCAATTTGTTCCATTGCACCAAAGATTGGCTGTACACCGGAGACTCTGCgtg                                                    >  1:574675/5‑150 (MQ=255)

                                                                                                ggATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCt                                                 <  1:1535963/150‑1 (MQ=255)

                                                                                                ggATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCt                                                 >  1:3427205/1‑150 (MQ=255)

                                                                                                ggATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCt                                                 <  1:2607530/150‑1 (MQ=255)

                                                                                                ggATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAACGGGCAGTCCGTATGGTTCTGGAAAGTCAGAGCGAATATGACTCACAATGGGCGACAATTTGTTCCACTGCTCCAAAGATTGGCTGTACGCCGGAGACTCTGCGTGTCt                                                 >  1:3747708/1‑150 (MQ=255)

                                                                                                 gATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCTGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTg                                                <  1:3133059/150‑1 (MQ=255)

                                                                                                 gATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTg                                                <  1:3090561/150‑1 (MQ=255)

                                                                                                 gATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTg                                                <  1:2858096/150‑1 (MQ=255)

                                                                                                   tGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTggg                                              <  1:472642/150‑1 (MQ=255)

                                                                                                     aCTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCATATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTggg                                              <  1:1683043/148‑1 (MQ=255)

                                                                                                      ctcaaaaTACTCGTTTTT‑CCCCCAAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGtac                                           >  1:2778654/4‑148 (MQ=255)

                                                                                                      cTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGtac                                           <  1:1586931/150‑3 (MQ=255)

                                                                                                        aaaaaTACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCACAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGtacac                                         >  1:1874446/1‑146 (MQ=255)

                                                                                                        aaaaaTACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTAc                                                                     >  1:4347710/1‑122 (MQ=255)

                                                                                                        aaaaaTACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGc                                         >  1:100152/1‑150 (MQ=255)

                                                                                                          aaaTACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAGAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCa                                       <  1:3934837/150‑1 (MQ=255)

                                                                                                          aaaTACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCa                                       <  1:201953/150‑1 (MQ=255)

                                                                                                           aaTACTCGTTTTT‑CCCCCGAATTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAg                                      <  1:4211877/150‑1 (MQ=255)

                                                                                                           aaTACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGATACGCCAg                                      <  1:1196826/150‑1 (MQ=255)

                                                                                                              aCTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCAt                                   <  1:1738341/150‑1 (MQ=255)

                                                                                                              aCTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCAt                                   >  1:1451878/1‑150 (MQ=255)

                                                                                                               cTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCgt                                                     >  1:4131558/1‑131 (MQ=255)

                                                                                                               cTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATg                                  >  1:11170/1‑150 (MQ=255)

                                                                                                                 cGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAg                                >  1:1658016/1‑150 (MQ=255)

                                                                                                                 cGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAg                                >  1:2335428/1‑150 (MQ=255)

                                                                                                                 cGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAg                                >  1:675722/1‑150 (MQ=255)

                                                                                                                    tttt‑CCCCCGGAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCgg                             >  1:2565424/1‑150 (MQ=255)

                                                                                                                     ttt‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCggg                            <  1:3017137/150‑1 (MQ=255)

                                                                                                                     ttt‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCggg                            <  1:771285/150‑1 (MQ=255)

                                                                                                                       t‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCGGGAt                          >  1:2285374/1‑150 (MQ=255)

                                                                                                                       t‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGGTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATGGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCGGGAt                          <  1:3957039/150‑1 (MQ=255)

                                                                                                                           cccGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTCCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCGGGATAcc                       >  1:1364186/1‑150 (MQ=255)

                                                                                                                           cccGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAA‑TCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCGGGATACCg                      <  1:538513/150‑1 (MQ=255)

                                                                                                                                aGTCCGTCAACGGGCAGTCCGTATGGTTCTGGAAAGTCAGAGCGAATATGACTCACAATGTGCGACAATTTGTTCCATTGCTCCAAAGATTGGCTGTACGCCGGAGACTCTGCGTGTCTGGGTTCGCCAGCATGAGCGGGATACCggggg                  <  1:2201185/150‑1 (MQ=255)

                                                                                                                                             gCAGTTCGTATGGTCATGGAAAGTCAGGGCGAATATGACTCACAATTGGCGACAATTTGTTCCATTGCTCCAAAGATTGGCTGTACGCCGGAGACTCTGCGTGTCTGGGTTCGCCAGCATGAGCGGGATACCGGGGGCGGTGagggaggg     <  1:3695615/150‑1 (MQ=255)

                                                                                                                                             gCAGTCCGTATGGTTCTGGAAAGTCAGAGCGAATATGACTCACAATGGGCGACAATTTGTTCCATTGCTCCAAAGATTGGCTGTACGCCGGAGACTCTGCGTGTCTGGGTTCGCCAGCATGAGCGGGATACCGGGGGCGGTGATGGAggg     >  1:2314234/1‑150 (MQ=255)

||

TGATTGTATGCCTGCGATCAATGCCAAACGTGTTTACCGGATCATGAACCGCCCCGGGAATCCTGGAGACTAAACTTCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAACGGGCAGTCCGTATGGTTCTGGAAAGTCAGAGCGAATATGACTCACAATGGGCGACAATTTGTTCCATTGCTCCAAAGATTGGCTGTACGCCGGAGACTCTGCGTGTCTGGGTTCGCCAGCATGAGCGGGATACCGGGGGCGGTGATGGAGGGCTC  >  Xen14_191/84‑376

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`