Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	Xen14_83	11,315	T→C	intergenic (+52/–)	Xen14_04442 → / –	hypothetical protein/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Xen14_83	11,315	0	T	C	100.0%	17.6 / NA	9	intergenic (+52/–)	Xen14_04442/–	hypothetical protein/–
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (6/3);  total (6/3)

AGTATCCCGGCGTGCTCGTTGAAATTTTACGTCGCGTCAATACGAGGACGCAGAAAGGGTGTGCTTTAAGCTTAACCGAAGCGTTTGATAGTTGATGACCTGCAGGCATGCAAGCTTGTGGCCGCGGCCTAGGGGGTCGAAGCTTGTATGCCTGCAGGTCGATTTTCGAGGACCCAGCTGTAATCCGG  >  Xen14_83/11169‑11356                                                                                                                                                   |                                          aGTATCCCGGCGTGCTCGTTGAAATTTTACGTCGCGTCAATACGAGGACGCAGAAAGGGTGTGCTTTAAGCTTAACCGAAGCGTTTGATAGTTGATGACCTGCAGGCATGCAAGCTTGCTGCCGCGGCCTAGGCGGCCGAAGCTTGCATg                                        <  1:541458/150‑1 (MQ=255) aGTATCCCGGCGTGCTCGTTGAAATTTTACGTCGCGTCAATACGAGGACGCAGAAAGGGTGTGCTTTAAGCTTAACCGAAGCGTTTGATAGTTGATGACCTGCAGGCATGCAAGCTTGCGGCCGCGGCCTAGGCGGCCGAAGCTTGCATg                                        <  1:541462/150‑1 (MQ=255)           cGTGCTCGTTGAAATTTTACGTCGCGTCAATACGAGGACGCAGAAAGGGTGTGCTTTAAGCTTAACCGAAGCGTTTGATAGTTGATGACCTGCAGGCATGCAAGCTTGCGGCTGCGGCCTAGGCGGCCGAAGTTTGCATGCCTGCAGGTc                              <  1:3410810/150‑1 (MQ=255)               cTCGTTGAAATTTTACGTCGCGTCAATACGAGGACGCAGAAAGGGTGTGCTTTAAGCTTAACCGAAGCGTTTGATAGTTGATGACCTGCAGGCATGCAAGCTTGCGGCCGCGGGCTAGGTGGCCAAAGCTTGCATGCCTGCAGGTCGaat                          >  1:2415320/1‑148 (MQ=255)                 cGTTGAAATTTTACGTCGCGTCAATACGAGGACGCAGAAAGGGTGTGCTTTAAGCTTAACCGAAGCGTTTGATAGTTGATGACCTGCAGGCATGCAAGCTTGCGGCCGCGGCCCAAGCGGCCGAAGCTTGCATGCCTGCAGGTCGaatct                        >  1:3163282/1‑146 (MQ=255)                  gTTGAAATTTTACGTCGCGTCAATACGAGGACGCAGAAAGGGTGTGCTTTAAGCTTAACCGAAGCGTTTGATAGTTGATGACCTGCAGGCCTACAAGCTTGCGGCCGCGGCCTAGGCGGCCAAAGCTTGCATGCCTTCAGGTCGactcta                       >  1:1986751/1‑145 (MQ=255)                                 cgcgTCAATACGAGGACGCAGAAAGGGTGTGCTTTAAGCTTAACCGAAGCGTTTGATAGTTGATGACCTGCAGGGATGCAAGCTTGCGGCCGCGGCCTAAGCGGCCAAAGCTTTCATGCCTGCAGGTTGAATCTGGAGGACCCGGCTGtc        >  1:1474756/1‑149 (MQ=255)                                       aaTACGAGGACGCAGAAAGGGTGTGCTTTAAGCTTAACCGAAGCGTTTGATAGTTGATGACCTGCAGACATGCAAGCTTGCGGCCGCGGCCTAGGCGGCCGAAGCTTGCATGCCTGCAGGTCGAATCTAGAGGATCCGGCTGTAATCCgg  >  1:2250947/1‑150 (MQ=255)                                               gACGCAGAAAGGGTGTGCTTTAAGCTTAACCGAAGCGTTTGATAGTTGATGACCTGCAGGCATGCAAGCTTGCGGCCGCGGCATAGGCGGCCGAAGCTTGCATg                                        >  1:3468709/1‑104 (MQ=255)                                                                                                                                                   |                                          AGTATCCCGGCGTGCTCGTTGAAATTTTACGTCGCGTCAATACGAGGACGCAGAAAGGGTGTGCTTTAAGCTTAACCGAAGCGTTTGATAGTTGATGACCTGCAGGCATGCAAGCTTGTGGCCGCGGCCTAGGGGGTCGAAGCTTGTATGCCTGCAGGTCGATTTTCGAGGACCCAGCTGTAATCCGG  >  Xen14_83/11169‑11356

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑