Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	Xen14_83	11,302	G→C	intergenic (+39/–)	Xen14_04442 → / –	hypothetical protein/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Xen14_83	11,302	0	G	C	93.3%	29.1 / NA	15	intergenic (+39/–)	Xen14_04442/–	hypothetical protein/–
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (5/9);  minor base T (1/0);  total (6/9)
Fisher's exact test for biased strand distribution p-value = 4.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TATGGTAAGTATCCCGGCGTGCTCGTTGAAATTTTACGTCGCGTCAATACGAGGACGCAGAAAGGGTGTGCTTTAAGCTTAACCGAAGCGTTTGATAGTTGATGACCTGCAGGCATGCAAGCTTGTGGCCGCGGCCTAGGGGGTCGAAGCTTGTATGCCTGCAGGTCGATTTTCGAGGACCCAGCTGTAATCCGG  >  Xen14_83/11162‑11356                                                                                                                                             |                                                       tatGGTAAGTATCCCGGCGTGCTCGTTGAAATTTTACGTCGCGTCAATACGAGGACGCAGAAAGGGTGTGCTTTAAGCTTAACCGAAGCGTTTGATAGTTGATGACCTGCAGGCATGCAAGCTTGCGGCCGCGGCCTAGGCGGCCGAAGc                                               <  1:4161702/150‑1 (MQ=255)    ggTAAGTATCCCGGCGTGCTCGTTGAAATTTTACGTCGCGTCAATACGAGGACGCAGAAAGGGTGTGCTTTAAGCTTAACCGAAGCGTTTGATAGTTGATGACCTGCAGGCATGCAAGCTTGCGGCCGCGGCCTAGGCGGCCGAAGCTTg                                            <  1:1489061/150‑1 (MQ=255)    ggTAAGTATCCCGGCGTGCTCGTTGAAATTTTACGTCGCGTCAATACGAGGACGCAGAAAGGGTGTGCTTTAAGCTTAACCGAAGCGTTTGATAGTTGATGACCTGCAGGCATGCAAGCTTGCGGCCGCGGCCTAGGCGGCCGAAGCTTg                                            <  1:1602963/150‑1 (MQ=255)    ggTAAGTATCCCGGCGTGCTCGTTGAAATTTTACGTCGCGTCAATACGAGGACGCAGAAAGGGTGTGCTTTAAGCTTAACCGAAGCGTTTGATAGTTGATGACCTGCAGGCATGCAAGCTTGCGGCCGCGGCCTAGGCGGCCGAAGCTTg                                            <  1:853085/150‑1 (MQ=255)      tAAGTATCCCGGCGTGCTCGTTGAAATTTTACGTCGCGTCAATACGAGGACGCAGAAAGGGTGTGCTTTAAGCTTAACCGAAGCGTTTGATAGTTGATGACCTGCAGGCATGCAAGCTTGTGGCCGCGGCCTAGGCGGCCGAAGCTTgca                                          <  1:3402619/150‑3 (MQ=255)      tAAGTATCCCGGCGTGCTCGTTGAAATTTTACGTCGCGTCAATACGAGGACGCAGAAAGGGTGTGCTTTAAGCTTAACCGAAGCGTTTGATAGTTGATGACCTGCAGGCATGCAAGCTTGCGGCCGCGGCCTAGGCGGCCGAAGCTTgca                                          <  1:2227503/150‑3 (MQ=255)        aGTATCCCGGCGTGCTCGTTGAAATTTTACGTCGCGTCAATACGAGGACGCAGAAAGGGTGTGCTTTAAGCTTAACCGAAGCGTTTGATAGTTGATGACCTGCAGGCATGCAAGCTTGCTGCCGCGGCCTAGGCGGCCGAAGCTTGCATg                                        <  1:541458/150‑1 (MQ=255)        aGTATCCCGGCGTGCTCGTTGAAATTTTACGTCGCGTCAATACGAGGACGCAGAAAGGGTGTGCTTTAAGCTTAACCGAAGCGTTTGATAGTTGATGACCTGCAGGCATGCAAGCTTGCGGCCGCGGCCTAGGCGGCCGAAGCTTGCATg                                        <  1:541462/150‑1 (MQ=255)                  cGTGCTCGTTGAAATTTTACGTCGCGTCAATACGAGGACGCAGAAAGGGTGTGCTTTAAGCTTAACCGAAGCGTTTGATAGTTGATGACCTGCAGGCATGCAAGCTTGCGGCTGCGGCCTAGGCGGCCGAAGTTTGCATGCCTGCAGGTc                              <  1:3410810/150‑1 (MQ=255)                      cTCGTTGAAATTTTACGTCGCGTCAATACGAGGACGCAGAAAGGGTGTGCTTTAAGCTTAACCGAAGCGTTTGATAGTTGATGACCTGCAGGCATGCAAGCTTGCGGCCGCGGGCTAGGTGGCCAAAGCTTGCATGCCTGCAGGTCGaat                          >  1:2415320/1‑148 (MQ=255)                        cGTTGAAATTTTACGTCGCGTCAATACGAGGACGCAGAAAGGGTGTGCTTTAAGCTTAACCGAAGCGTTTGATAGTTGATGACCTGCAGGCATGCAAGCTTGCGGCCGCGGCCCAAGCGGCCGAAGCTTGCATGCCTGCAGGTCGaatct                        >  1:3163282/1‑146 (MQ=255)                         gTTGAAATTTTACGTCGCGTCAATACGAGGACGCAGAAAGGGTGTGCTTTAAGCTTAACCGAAGCGTTTGATAGTTGATGACCTGCAGGCCTACAAGCTTGCGGCCGCGGCCTAGGCGGCCAAAGCTTGCATGCCTTCAGGTCGactcta                       >  1:1986751/1‑145 (MQ=255)                                        cgcgTCAATACGAGGACGCAGAAAGGGTGTGCTTTAAGCTTAACCGAAGCGTTTGATAGTTGATGACCTGCAGGGATGCAAGCTTGCGGCCGCGGCCTAAGCGGCCAAAGCTTTCATGCCTGCAGGTTGAATCTGGAGGACCCGGCTGtc        >  1:1474756/1‑149 (MQ=255)                                              aaTACGAGGACGCAGAAAGGGTGTGCTTTAAGCTTAACCGAAGCGTTTGATAGTTGATGACCTGCAGACATGCAAGCTTGCGGCCGCGGCCTAGGCGGCCGAAGCTTGCATGCCTGCAGGTCGAATCTAGAGGATCCGGCTGTAATCCgg  >  1:2250947/1‑150 (MQ=255)                                                      gACGCAGAAAGGGTGTGCTTTAAGCTTAACCGAAGCGTTTGATAGTTGATGACCTGCAGGCATGCAAGCTTGCGGCCGCGGCATAGGCGGCCGAAGCTTGCATg                                        >  1:3468709/1‑104 (MQ=255)                                                                                                                                             |                                                       TATGGTAAGTATCCCGGCGTGCTCGTTGAAATTTTACGTCGCGTCAATACGAGGACGCAGAAAGGGTGTGCTTTAAGCTTAACCGAAGCGTTTGATAGTTGATGACCTGCAGGCATGCAAGCTTGTGGCCGCGGCCTAGGGGGTCGAAGCTTGTATGCCTGCAGGTCGATTTTCGAGGACCCAGCTGTAATCCGG  >  Xen14_83/11162‑11356

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑