Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	Xen14_83	11,287	T→C	intergenic (+24/–)	Xen14_04442 → / –	hypothetical protein/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Xen14_83	11,287	0	T	C	90.9%	73.3 / ‑6.5	33	intergenic (+24/–)	Xen14_04442/–	hypothetical protein/–
Reads supporting (aligned to +/- strand):  ref base T (2/1);  new base C (9/21);  total (11/22)
Fisher's exact test for biased strand distribution p-value = 2.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCGGGATAATTAGCAGAGAATATGGTAAGTATCCCGGCGTGCTCGTTGAAATTTTACGTCGCGTCAATACGAGGACGCAGAAAGGGTGTGCTTTAAGCTTAACCGAAGCGTTTGATAGTTGATGACCTGCAGGCATGCAAGCTTGTGGCCGCGGCCTAGGGGGTCGAAGCTTGTATGCCTGCAGGTCGATTTTCGAGGACCCAGCTGTAATCCGG  >  Xen14_83/11142‑11356                                                                                                                                                  |                                                                      tCGGGATAATTAGCAGAGAATATGGTAAGTATCCCGGCGTGCTCGTTGAAATTTTACGTCGCGTCAATACGAGGACGCAGAAAGGGTGTGCTTTAAGCTTAACCGAAGCGTTTGATAGTTGATGACCTGCAGGCATGCAAGCTTGCGGcc                                                                   <  1:2592742/150‑1 (MQ=255)  cGGGATAATTAGCAGAGAATATGGTAAGTATCCCGGCGTGCTCGTTGAAATTTTACATCGCGTCAATACGAGGACGCAGAAAGGGTGTGCTTTAAGCTTAACCGAAGCGTTTGATAGTTGATGACCTGCAAGCATACAAGCTTGTGTCcg                                                                  >  1:2611558/1‑150 (MQ=255)   gggATAATTAGCAGAGAATATGGTAAGTATCCCGGCGTGCTCGTTGAAATTTTACGTCGCGTCAATACGAGGACGCAGAAAGGGTGTGCTTTAAGCTTAACCGAAGCGTTTGATAGTTGATGACCTGCAGGCATGCAGGCTTGCGGCcgc                                                                 <  1:4104947/150‑1 (MQ=255)   gggATAATTAGCAGAGAATATGGTAAGTATCCCGGCGTGCTCGTTGAAATTTTACGTCGCGTCAATACGAGGACGCAGAAAGGGTGTGCTTTAAGCTTAACCGAAGCGTTTGATAGTTGATGACCTGCAGGCATGCAAGCTTGCGGCcgc                                                                 <  1:1170160/150‑1 (MQ=255)    ggATAATTAGCAGAGAATATGGTAAGTATCCCGGCGTGCTCGTTGAAATTTTACGTCGCGTCAATACGAGGACGCAGAAAGGGTGTGCTTTAAGCTTAACCGAAGCGTTTGATAGTTGATGACCTGCAGGCATGCAAGCTTGCGGCcgcg                                                                <  1:309832/150‑1 (MQ=255)    ggATAATTAGCAGAGAATATGGTAAGTATCCCGGCGTGCTCGTTGAAATTTTACGTCGCGTCAATACGAGGACGCAGAAAGGGTGTGCTTTAAGCTTAACCGAAGCGTTTGATAGTTGATGACCTGCAGGCATGCAAGCTTGCGGCcgcg                                                                <  1:309830/150‑1 (MQ=255)     gATAATTAGCAGAGAATATGGTAAGTATCCCGGCGTGCTCGTTGAAATTTTACGTCGCGTCAATACGAGGACGCAGAAAGGGTGTGCTTTAAGCTTAACCGAAGCGTTTGATAGTTGATGACCTGCAGGCATGCAAGCTTGCGGCCGCgg                                                               <  1:1852711/150‑1 (MQ=255)      aTAATTAGCAGAGAATATGGTAAGTATCCCGGCGTGCTCGTTGAAATTTTACGTCGCGTCAATACGAGGACGCAGAAAGGGTGTGCTTTAAGCTTAACCGAAGCGTTTGATAGTTGATGACCTGCAGGCATGCAAGCTTGCGGCCGCGGc                                                              >  1:3412654/1‑150 (MQ=255)      aTAATTAGCAGAGAATATGGTAAGTATCCCGGCGTGCTCGTTGAAATTTTACGTCGCGTCAATACGAGGACGCAGAAAGGGTGTGCTTTAAGCTTAACCGAAGCGTTTGATAGTTGATGACCTGCAGGCATGCAAGCTTGCGGCCGCGGc                                                              >  1:3412655/1‑150 (MQ=255)      aTAATTAGCAGAGAATATGGCAAGTATCCCGGCGTGCTCGTTGAAATTTTACGTCGCGTCAATACGAGGACGCAGAAAGGGTGTGCTTTAAGCTTAACCGAAGCGTTTGATAGTTGATGACCTGCAGGCATGCAAGCTTGCTGCCGCGGc                                                              <  1:2644180/150‑1 (MQ=255)      aTAATTAGCAGAGAATATGGCAAGTATCCCGGCGTGCTCGTTGAAATTTTACGTCGCGTCAATACGAGGACGCAGAAAGGGTGTGCTTTAAGCTTAACCGAAGCGTTTGATAGTTGATGACCTGCAGGCATGCAAGCTTGCGGCCGCGGc                                                              <  1:2694242/150‑1 (MQ=255)          ttAGCAGAGAATATGGTAAGTATCCCGGCGTGCTCGTTGAAATTTTACGTCGCGTCAATACGAGGACGCAGAAAGGGTGTGCTTTAAGCTTAACCGAAGCGTTTGATAGTTGATGACCTGCAGGCATGCCAGTTTGTGGCCGCGGCCTAg                                                          >  1:2060087/1‑150 (MQ=255)          ttAGCAGAGAATATGGTAAGTATCCCGGCGTGCTCGTTGAAATTTTACGTCGCGTCAATACGAGGACGCAGAAAGGGTGTGCTTTAAGCTTAACCGAAGCGTTTGATAGTTGATGACCTGCAGGCATGCAAGCTTGCGGCCGCGGACTAg                                                          >  1:2370884/1‑150 (MQ=255)          tgaGCAGAGAATATGGTAAGTATCCCGGCGTGCTCGTTGAAATTTTACGTCGCTTCAATACGAGGACGCAGAAAGGGTGTGCTTTAAGCTTAACCGAAGCGTTTGATAGTTGATGACCGGCAGGCATGCAAGCTTGCGGCCGCgg                                                               <  1:3325364/143‑1 (MQ=255)             gCAGAGAATATGGTAAGTATCCCGGCGTGCTCGTTGAAATTTTACGTCGCGTCAATACGAGGACGCAGAAAGGGTGTGCTTTAAGCTTAACCGAAGCGTTTGATAGTTGATGACCTGCAGGCATGCAAGCTTGCGGCCGCGGCCTAggcg                                                       <  1:4106861/150‑3 (MQ=255)             gCAGAGAATATGGTAAGTATCCCGGCGTGCTCGTTGAAATTTTACGTCGCGTCAATACGAGGACGCAGAAAGGGTGTGCTTTAAGCTTAACCGAAGCGTTTGATAGTTGATGACCTGCAGGCATGCAAGCTTGCGGCCGCGGCCTAggcg                                                       <  1:2866277/150‑3 (MQ=255)               agagAATATGGTAAGTATCCCGGCGTGCTCGTTGAAATTTTACGTCGCGTCAATACGAGGACGCAGAAAGGGTGTGCTTTAAGCTTAACCGAAGCGTTTGATAGTTGATGACCTGCAGGCATGCAAGCTTGCGGCCGCGGCCTAggcggc                                                     <  1:2949626/150‑5 (MQ=255)                gagaATATGGTAAGTATCCCGGCGTGCTCGTTGAAATTTTACGTCGCGTCAATACGAGGACGCAGAAAGGGTGTGCTTTAAGCTTAACCGAAGCGTTTGATAGTTGATGACCTGCAGGCATGCAAGCTTGCGGCCGCGGCCTAggcggcc                                                    <  1:4263896/150‑6 (MQ=255)                     tatGGTAAGTATCCCGGCGTGCTCGTTGAAATTTTACGTCGCGTCAATACGAGGACGCAGAAAGGGTGTGCTTTAAGCTTAACCGAAGCGTTTGATAGTTGATGACCTGCAGGCATGCAAGCTTGCGGCCGCGGCCTAGGCGGCCGAAGc                                               <  1:4161702/150‑1 (MQ=255)                        ggTAAGTATCCCGGCGTGCTCGTTGAAATTTTACGTCGCGTCAATACGAGGACGCAGAAAGGGTGTGCTTTAAGCTTAACCGAAGCGTTTGATAGTTGATGACCTGCAGGCATGCAAGCTTGCGGCCGCGGCCTAGGCGGCCGAAGCTTg                                            <  1:853085/150‑1 (MQ=255)                        ggTAAGTATCCCGGCGTGCTCGTTGAAATTTTACGTCGCGTCAATACGAGGACGCAGAAAGGGTGTGCTTTAAGCTTAACCGAAGCGTTTGATAGTTGATGACCTGCAGGCATGCAAGCTTGCGGCCGCGGCCTAGGCGGCCGAAGCTTg                                            <  1:1602963/150‑1 (MQ=255)                        ggTAAGTATCCCGGCGTGCTCGTTGAAATTTTACGTCGCGTCAATACGAGGACGCAGAAAGGGTGTGCTTTAAGCTTAACCGAAGCGTTTGATAGTTGATGACCTGCAGGCATGCAAGCTTGCGGCCGCGGCCTAGGCGGCCGAAGCTTg                                            <  1:1489061/150‑1 (MQ=255)                          tAAGTATCCCGGCGTGCTCGTTGAAATTTTACGTCGCGTCAATACGAGGACGCAGAAAGGGTGTGCTTTAAGCTTAACCGAAGCGTTTGATAGTTGATGACCTGCAGGCATGCAAGCTTGTGGCCGCGGCCTAGGCGGCCGAAGCTTgca                                          <  1:3402619/150‑3 (MQ=255)                          tAAGTATCCCGGCGTGCTCGTTGAAATTTTACGTCGCGTCAATACGAGGACGCAGAAAGGGTGTGCTTTAAGCTTAACCGAAGCGTTTGATAGTTGATGACCTGCAGGCATGCAAGCTTGCGGCCGCGGCCTAGGCGGCCGAAGCTTgca                                          <  1:2227503/150‑3 (MQ=255)                            aGTATCCCGGCGTGCTCGTTGAAATTTTACGTCGCGTCAATACGAGGACGCAGAAAGGGTGTGCTTTAAGCTTAACCGAAGCGTTTGATAGTTGATGACCTGCAGGCATGCAAGCTTGCTGCCGCGGCCTAGGCGGCCGAAGCTTGCATg                                        <  1:541458/150‑1 (MQ=255)                            aGTATCCCGGCGTGCTCGTTGAAATTTTACGTCGCGTCAATACGAGGACGCAGAAAGGGTGTGCTTTAAGCTTAACCGAAGCGTTTGATAGTTGATGACCTGCAGGCATGCAAGCTTGCGGCCGCGGCCTAGGCGGCCGAAGCTTGCATg                                        <  1:541462/150‑1 (MQ=255)                                      cGTGCTCGTTGAAATTTTACGTCGCGTCAATACGAGGACGCAGAAAGGGTGTGCTTTAAGCTTAACCGAAGCGTTTGATAGTTGATGACCTGCAGGCATGCAAGCTTGCGGCTGCGGCCTAGGCGGCCGAAGTTTGCATGCCTGCAGGTc                              <  1:3410810/150‑1 (MQ=255)                                          cTCGTTGAAATTTTACGTCGCGTCAATACGAGGACGCAGAAAGGGTGTGCTTTAAGCTTAACCGAAGCGTTTGATAGTTGATGACCTGCAGGCATGCAAGCTTGCGGCCGCGGGCTAGGTGGCCAAAGCTTGCATGCCTGCAGGTCGaat                          >  1:2415320/1‑148 (MQ=255)                                            cGTTGAAATTTTACGTCGCGTCAATACGAGGACGCAGAAAGGGTGTGCTTTAAGCTTAACCGAAGCGTTTGATAGTTGATGACCTGCAGGCATGCAAGCTTGCGGCCGCGGCCCAAGCGGCCGAAGCTTGCATGCCTGCAGGTCGaatct                        >  1:3163282/1‑146 (MQ=255)                                             gTTGAAATTTTACGTCGCGTCAATACGAGGACGCAGAAAGGGTGTGCTTTAAGCTTAACCGAAGCGTTTGATAGTTGATGACCTGCAGGCCTACAAGCTTGCGGCCGCGGCCTAGGCGGCCAAAGCTTGCATGCCTTCAGGTCGactcta                       >  1:1986751/1‑145 (MQ=255)                                                            cgcgTCAATACGAGGACGCAGAAAGGGTGTGCTTTAAGCTTAACCGAAGCGTTTGATAGTTGATGACCTGCAGGGATGCAAGCTTGCGGCCGCGGCCTAAGCGGCCAAAGCTTTCATGCCTGCAGGTTGAATCTGGAGGACCCGGCTGtc        >  1:1474756/1‑149 (MQ=255)                                                                  aaTACGAGGACGCAGAAAGGGTGTGCTTTAAGCTTAACCGAAGCGTTTGATAGTTGATGACCTGCAGACATGCAAGCTTGCGGCCGCGGCCTAGGCGGCCGAAGCTTGCATGCCTGCAGGTCGAATCTAGAGGATCCGGCTGTAATCCgg  >  1:2250947/1‑150 (MQ=255)                                                                          gACGCAGAAAGGGTGTGCTTTAAGCTTAACCGAAGCGTTTGATAGTTGATGACCTGCAGGCATGCAAGCTTGCGGCCGCGGCATAGGCGGCCGAAGCTTGCATg                                        >  1:3468709/1‑104 (MQ=255)                                                                                                                                                  |                                                                      TCGGGATAATTAGCAGAGAATATGGTAAGTATCCCGGCGTGCTCGTTGAAATTTTACGTCGCGTCAATACGAGGACGCAGAAAGGGTGTGCTTTAAGCTTAACCGAAGCGTTTGATAGTTGATGACCTGCAGGCATGCAAGCTTGTGGCCGCGGCCTAGGGGGTCGAAGCTTGTATGCCTGCAGGTCGATTTTCGAGGACCCAGCTGTAATCCGG  >  Xen14_83/11142‑11356

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑