Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	Xen14_65	17,250	G→A	intergenic (+111/–)	gmd_2 → / –	GDP‑mannose 4,6‑dehydratase/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Xen14_65	17,250	0	G	A	100.0%	154.6 / NA	57	intergenic (+111/–)	gmd_2/–	GDP‑mannose 4,6‑dehydratase/–
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/35);  total (22/35)

GGAGTCATAAGCATGAGTAGACAACGCATTTTTATCGCTGGCCATCGCGGAATGGTCGGTTCTGCCATCAGGCGGCAGCTCGAACAGCGCGGTGATGTGGAACTGGTATTACGCACCCGCGACGAGCTGAACCTGCTGGACAGCCGCGCGGTGCATGATTTCTTTGCCAGCGAACGCATTGACCAGGTCTATCTGGCGGCGGCGAAAGTGGGCGGCATTGTTGCCAACAACACCTATCCGGCGGATTTCATCTACCAGAACA  >  Xen14_65/17130‑17391                                                                                                                         |                                                                                                                                              ggAGTCATAAGCATGAGTAGACAACGCATTTTTATCGCTGGCCATCGCGGAATGGTCGGTTCTGCCATCAGGCGGCAGCTCGAACAGCGCGGTGATGTGGAACTGGTATTACGCACCCGCAACGAGCTGAACCTGCTGGACAGCcgcgcg                                                                                                                  <  1:1409170/150‑1 (MQ=14) ggAGTCATAAGCATGAGTAGACAACGCATTTTTATCGCTGGCCATCGCGGAATGGTCGGTTCTGCCATCAGGCGGCAGCTCGAACAGCGCGGTGATGTGGAACTGGTATTACGCACCCGCAACGAGCTGAACCTGCTGGACAGCcgcgcg                                                                                                                  >  1:3180991/1‑150 (MQ=14)  gAGTCATAAGCATGAGTAGACAACGCATTTTTATCGCTGGCCATCGCGGAATGGTCGGTTCTGCCATCAGGCGGCAGCTCGAACAGCGCGGTGATGTGGAACTGGTATTACGCACCCGCAACGAGCTGAACCTGCTGGACAGCCGCGCgg                                                                                                                 <  1:970883/150‑1 (MQ=17)      cATAAGCATGAGTAGACAACGCATTTTTATCGCTGGCCATCGCGGAATGGTCGGTTCTGCCATCAGGCGGCAGCTCGAACAGCGCGGTGATGTGGAACTGGTATTACGCACCCGCAACGAGCTGAACCTGCTGGACAGCCGCGCGGTGCa                                                                                                             <  1:570484/150‑1 (MQ=25)      cATAAGCATGAGTAGACAACGCATTTTTATCGCTGGCCATCGCGGAATGGTCGGTTCTGCCATCAGGCGGCAGCTCGAACAGCGCGGTGATGTGGAACTGGTATTACGCACCCGCAACGAGCTGAACCTGCTGGACAGCCGCGCGGTGCa                                                                                                             >  1:2298540/1‑150 (MQ=25)      cATAAGCATGAGTAGACAACGCATTTTTATCGCTGGCCATCGCGGAATGGTCGGTTCTGCCATCAGGCGGCAGCTCGAACAGCGCGGTGATGTGGAACTGGTATTACGCACCCGCAACGAGCTGAACCTGCTGGACAGCCGCGCGGTGCa                                                                                                             >  1:2298539/1‑150 (MQ=25)        tAAGCATGAGTAGACAACGCATTTTTATCGCTGGCCATCGCGGAATGGTCGGTTCTGCCATCAGGCGGCAGCTCGAACAGCGCGGTGATGTGGAACTGGTATTACGCACCCGCAACGAGCTGAACCTGCTGGACAGCCGCGCGGTGCATg                                                                                                           <  1:3308402/150‑1 (MQ=25)            cATGAGTAGACAACGCATTTTTATCGCTGGCCATCGCGGAATGGTCGGTTCTGCCATCAGGCGGCAGCTCGAACAGCGCGGTGATGTGGAACTGGTATTACGCACCCGCAACGAGCTGAACCTGCTGGACAGCCGCGCGGTGCATGAttt                                                                                                       <  1:3772585/150‑1 (MQ=25)            cATGAGTAGACAACGCATTTTTATCGCTGGCCATCGCGGAATGGTCGGTTCTGCCATCAGGCGGCAGCTCGAACAGCGCGGTGATGTGGAACTGGTATTACGCACCCGCAACGAGCTGAACCTGCTGGACAGCCGCGCGGTGCATGAttt                                                                                                       <  1:3566339/150‑1 (MQ=25)            cATGAGTAGACAACGCATTTTTATCGCTGGCCATCGCGGAATGGTCGGTTCTGCCATCAGGCGGCAGCTCGAACAGCGCGGTGATGTGGAACTGGTATTACGCACCCGCAACGAGCTGAACCTGCTGGACAGCCGCGCGGTGCATGAttt                                                                                                       <  1:759961/150‑1 (MQ=25)            cATGAGTAGACAACGCATTTTTATCGCTGGCCATCGCGGAATGGTCGGTTCTGCCATCAGGCGGCAGCTCGAACAGCGCGGTGATGTGGAACTGGTATTACGCACCCGCAACGAGCTGAACCTGCTGGACAGCCGCGCGGTGCATGAttt                                                                                                       <  1:759962/150‑1 (MQ=25)            cATGAGTAGACAACGCATTTTTATCGCTGGCCATCGCGGAATGGTCGGTTCTGCCATCAGGCGGCAGCTCGAACAGCGCGGTGATGTGGAACTGGTATTACGCACCCGCAACGAGCTGAACCTGCTGGACAGCCGCGCGGTGCATGAttt                                                                                                       <  1:759963/150‑1 (MQ=25)            cATGAGTAGACAACGCATTTTTATCGCTGGCCATCGCGGAATGGTCGGTTCTGCCATCAGGCGGCAGCTCGAACAGCGCGGTGATGTGGAACTGGTATTACGCACCCGCAACGAGCTGAACCTGCTGGACAGCCGCGCGGTGCATGAttt                                                                                                       <  1:2256366/150‑1 (MQ=25)                   aGACAACGCATTTTTATCGCTGGCCATCGCGGAATGGTCGGTTCTGCCATCAGGCGGCAGCTCGAACAGCGCGGTGATGTGGAACTGGTATTACGCACCCGCAACGAGCTGAACCTGCTGGACAGCCGCGCGGTGCATGATTTCTTTGcc                                                                                                <  1:4084146/150‑1 (MQ=25)                     aCAACGCATTTTTATCGCTGGCCATCGCGGAATGGTCGGTTCTGCCATCAGGCGGCAGCTCGAACAGCGCGGTGATGTGGAACTGGTATTACGCACCCGCAACGAGCTGAACCTGCTGGACAGCCGCGCGGTGCATGATTTCttt                                                                                                   <  1:2078255/145‑1 (MQ=25)                     aCAACGCATTTTTATCGCTGGCCATCGCGGAATGGTCGGTTCTGCCATCAGGCGGCAGCTCGAACAGCGCGGTGATGTGGAACTGGTATTACGCACCCGCAACGAGCTGAACCTGCTGGACAGCCGCGCGGTGCATGATTTCTTTGCCAg                                                                                              <  1:3123276/150‑1 (MQ=25)                        aCGCATTTTTATCGCTGGCCATCGCGGAATGGTCGGTTCTGCCATCAGGCGGCAGCTCGAACAGCGCGGTGATGTGGAACTGGTATTACGCACCCGCAACGAGCTGAACCTGCTGGACAGCCGCGCGGTGCATGATTTCTTTGCCAGCGa                                                                                           >  1:3476763/1‑150 (MQ=25)                           cATTTTTATTGCTGGCCATCGCGGAATGGTCGGTTCTGCCATCAGGCGGCAGCTCGAACAGCGCGTTGATGTGGAACTGGTATTACGCACCCGCAACGAGCTGAACCTGCTGGACAGCCGCGCGGTGCATGATTTCTTTGCCAGCGAACg                                                                                        <  1:3871590/150‑1 (MQ=255)                           cATTTTTATCGCTGGCCATCGCGGAATGGTCGGTTCTGCCATCAGGCGGCAGCTCGAACAGCGCGGTGATGTGGAACTGGTATTACGCACCCGCAACGAGCTGAACATGCTGGACAGCCGCGCGGTGCATGATTTCTTTGCCAGCGAACg                                                                                        <  1:1646655/150‑1 (MQ=255)                               tttATCGCTGGCCATCGCGGAATGGTCGGTTCTGCCGTCAGGCGGCAGCTCGAACAGCGCGGTGATGTGGAACTGTTATTCCGCACCCGCAACGAGCTGAACCTGCTGGACAGCCGCGCGGTGCATGATTTCTTTGCCAGCGAACGCAtt                                                                                    >  1:3665877/1‑150 (MQ=14)                               tttATCGCTGGCCATCGCGGAATGGTCGGTTCTGCCATCAGGCGGCAGCTCGAACAGCGCGGTGATGTGGAACTGGTATTACGCACCCGCAACGAGCTGAACCTGCTGGACAGCCGCGCGGTGCATGATTTCTTTGCCAGCGAACGCAtt                                                                                    >  1:3433806/1‑150 (MQ=255)                               tttATCGCTGGCCATCGCGGAATGGTCGGTTCTGCCATCAGGCGGCAGCTCGAACAGCGCGGTGATGTGGAACTGGTATTACGCACCCGCAACGAGCTGAACCTGCTGGACAGCCGCGCGGTGCATGATTTCTTTGCCAGCGAACGCAtt                                                                                    >  1:3433804/1‑150 (MQ=255)                               tttATCGCTGGCCATCGCGGAATGGTCGGTTCTGCCATCAGGCGGCAGCTCGAACAGCGCGGTGATGTGGAACTGGTATTACGCACCCGCAACGAGCTGAACCTGCTGGACAGCCGCGCGGTGCATGATTTCTTTGCCAGCGAACGCAtt                                                                                    >  1:2892040/1‑150 (MQ=255)                                ttATCGCTGGCCATCGCGGAATGGTCGGTTCTGCCATCAGGCGGCAGCTCGAACAGCGCGGTGATGTGGAACTGGTATTACGCACCCGCAACGAGCTGAACCTGCTGGACAGCCGCGCGGTGCATGATTTCTTTGCCAGCGAACGCATTg                                                                                   <  1:166229/150‑1 (MQ=255)                                 tATCGCTGGCCATCGCGGAATGGTCGGTTCTGCCATCAGTCGGCAGCTCGAACAGCGCGGTGATGTGGAACTGGTATTACGCACCCGCAACGAGCTGAACCTGCTGGACAGCCGCGCGGTGCATGATTTCTTTGCCAGCGAACGCATTGa                                                                                  <  1:183316/150‑1 (MQ=255)                                   tCGCTGGCCATCGCGGAATGGTCGGTTCTGCCATCAGGCGGCAGCTCGAACAGCGCGGTGATGTGGAACTGGTATTACGCACCCGCAACGAGCTGAACCTGCTGGACAGCCGCGCGGTGCATGATTTCTTTGCCAGCg                                                                                            <  1:1872639/138‑1 (MQ=37)                                       tGGCCATCGCGGAATGGTCGGTTCTGCCATCAGGCGGCAGCTCGAACAGCGCGGTGATGTGGAACTGGTATTACGCACCCGCAACGAGCTGAACCTGCTGGACAGCCGCGCGGTGCATGATTTCTTTGCCAGCGAACGCATTGACCAGGt                                                                            <  1:2476243/150‑1 (MQ=255)                                                       gTCGGTTCTGCCATCAGGCGGCAGCTCGAACAGCGCGGTGATGTGGAACTGGTATTACGCACCCGCAACGAGCTGAACCTGCTGGACAGCCGCGCGGTGCATGATTTCTTTGCCAGCGAACGCATTGACCAGGTCTATCTGgcggcggcg                                                            >  1:1255491/1‑150 (MQ=255)                                                       gTCGGTTCTGCCATCAGGCGGCAGCTCGAACAGCGCGGTGATGTGGAACTGGTATTACGCACCCGCAACGAGCTGAACCTGCTGGACAGCCGCGCGGTGCATGATTTCTTTGCCAGCGAACGCATTGACCAGGTCTATCTGgcggcggcg                                                            >  1:3145871/1‑150 (MQ=255)                                                         cGGTTCTGCTATCAGGCGGCAGCTCGAACAGCGCGGTGATGTGGAACTGGTATTACGCACCCGCAACGAGCTGAACCTGCTGGACAGCCGCGCGGTGCATGATTTCTTTGCCAGCGAACGCATTGACCAGGTCTATCTGGCGGCGGCGaa                                                          <  1:2199432/150‑1 (MQ=255)                                                         cGGTTCTGCCATCAGGCGGCAGCTCGAACAGCGCGGTGATGTGGAACTGGTATTACGCACCCGCAACGAGCTGAACCTGCTGGACAGCCGCGCGGTGCATGATTTCTTTGCCAGCGAACGCATTGACCAGGTCTATCTGGCGGCGGCGaa                                                          >  1:1976065/1‑150 (MQ=255)                                                         cGGTTCTGCCATCAGGCGGCAGCTCGAACAGCGCGGTGATGTGGAACTGGTATTACGCACCCGCAACGAGCTGAACCTGCTGGACAGCCGCGCGGTGCATGATTTCTTTGCCAGCGAACGCATTGACCAGGTCTATCTGGCGGCGGCGaa                                                          <  1:2199435/150‑1 (MQ=255)                                                          ggTTCTGCCATCAGGCGGCAGCTCGAACAGCGCGGTGATGTGGAACTGGTATTACGCACCCGCAACGAGCTGAACCTGCTGGACAGCCGCGCGGTGCATGATTTCTTTGCCAGCGAACGCATTGACCAGGTCTATCTGGCGGCGGCGaaa                                                         <  1:395957/150‑1 (MQ=255)                                                          ggTTCTGCCATCAGGCGGCAGCTCGAACAGCGCGGTGATGTGGAACTGGTATTACGCACCCGCAACGAGCTGAACCTGCTGGACAGCCGCGCGGTGCATGATTTCTTTGCCAGCGAACGCATTGACCAGGTCTATCTGGCGGCGGCGaaa                                                         <  1:2759352/150‑1 (MQ=255)                                                           gTTCTGCCATCAGGCGGCAGCTCGAACAGCGCGGTGATGTGGAACTGGTATTACGCACCCGCAACGAGCTGAACCTGCTGGACAGCCGCGCGGTGCATGATTTCTTTGCCAGCGAACGCATTGACCAGGTCTATCTGGCGGCGGCGAAAg                                                        <  1:1650396/150‑1 (MQ=255)                                                                gCCATCAGGCGGCAGCTCGAACAGCGCGGTGATGTGGAACTGGTATTACGCACCCGCAACGAGCTGAACCTGCTGGACAGCCGCGCGGTGCATGATTTCTTTGCCAGCGAACGCATTGACCAGGTCTATCTGGCGGCGGCGAAAGTGggc                                                   >  1:4061842/1‑150 (MQ=255)                                                                          ggcAGCTCGAACAGCGCGGTGATGTGGAACTGGTATTACGCACCCGCAACGAGCTGAACCTGCTGGACAGCCGCGCGGTGCATGATTTCTTTGCCAGCGAACGCATTGACCAGGTCTATCTGGCGGCGGCGAAAGTGGGCGGCAttgttg                                         <  1:4026899/150‑1 (MQ=255)                                                                          ggcAGCTCGAACAGCGCGGTGATGTGGAACTGGTATTACGCACCCGCAACGAGCTGAACCTGCTGGACAGCCGCGCGGTGCATGATTTCTTTGCCAGCGAACGCATTGACCAGGTCTATCTGGCGGCGGCGAAAGTGGGCGGCAttgttg                                         >  1:1391133/1‑150 (MQ=255)                                                                               cTCGAACAGCGCGGTGATGTGGAACTGGTATTACGCACCCGCAACGAGCTGAACCTGCTGGACAGCCGCGCGGTGCATGATTTCTTTGCCAGCGAACGCATTGACCAGGTCTATCTGGCGGCGGCGAAAGTGGGCGGCATTGTTGCcaac                                    <  1:3778578/150‑1 (MQ=255)                                                                                tCGAACAGCGCGGTGATGTGGAACTGGTATTACGCACCCGCAACGAGCTGAACCTGCTGGACAGCCGCGCGGTGCATGATTTCTTTGCCAGCGAACGCATTGACCAGGTCTATCTGGCGGCGGCGAAAGTGGGCGGCATTGTTGCcaaca                                   >  1:290902/1‑150 (MQ=255)                                                                                 cGAACAGCGCGGTGATGTGGAACTGGTATTACGCACCCGCAACGAGCTGAACCTGCTGGACAGCCGCGCGGTGCATGATTTCTTTGCCAGCGAACGCATTGACCAGGTCTATCTGGCGGCGGCGAAAGTGGGCGGCATTGTTGCcaggac                                  >  1:3218242/1‑146 (MQ=255)                                                                                  gAACAGCGCGGTGATGTGGAACTGGTATTACGCACCCGCAACGAGCTGAACCTGCTGGACAGCCGCGCGGTGCATGATTTCTTTGCCAGCGAACGCATTGACCAGGTCTATCTGGCGGCGGCGAAAGTGGGCGGCATTGTTGCCaacaac                                 <  1:3454211/150‑1 (MQ=255)                                                                                     cAGCGCGGTGATGTGGAACTGGTATTACGCACCCGCAACGAGCTGAACCTGCTGGACAGCCGCGCGGTGCATGATTTCTTTGCCAGCGAACGCATTGACCAGGTCTATCTGGCGGCGGCGAAAGTGGGCGGCATTGTTGCCAACAACAcc                              >  1:3455735/1‑150 (MQ=255)                                                                                              gATGTGGAACTGGTATTACGCACCCGCAACGAGCTGAACCTGCTGGACAGCCGCGCGGTGCATGATTTCTTTGCCAGCGAACGCATTGACCAGGTCTATCTGGCGGCGGCGAAAGTGGGCGGCATTGTTGCCAACAACACCTATCcggcg                     >  1:3207773/1‑150 (MQ=255)                                                                                              gATGTGGAACTGGTATTACGCACCCGCAACGAGCTGAACCTGCTGGACAGCCGCGCGGTGCATGATTTCTTTGCCAGCGAACGCATTGACCAGGTCTATCTGGCGGCGGCGAAAGTGGGCGGCATTGTTGCCAACAACACCTATCcggcg                     <  1:1461404/150‑1 (MQ=255)                                                                                               aTGTGGAACTGGTATTACGCACCCGCAACGAGCTGAACCTGCTGGACAGCCGCGCGGTGCATGATTTCTTTGCCAGCGAACGCATTGACCAGGTCTATCTGGCGGCGGCGAAAGTGGGCGGCATTGTTGCCAACAACACCTATCcggcgg                    <  1:3796291/150‑1 (MQ=255)                                                                                                 gtgGAACTGGTATTACGCACCCGCAACGAGCCGAACCTGCTGGACAGCCGCGCGGTGCATGATTTCTTTGCCAGCGAACGCATTGACCAGGTCTATCTGGCGGCGGCGAAAGTGGGCGGCATTGTTGCCAACAACACCTATCCGGCGGAt                  >  1:1413751/1‑150 (MQ=255)                                                                                                   ggAACTGGTATTACGCACCCGCAACGAGTTGAACCTGCTGGACAGCCGCGCGGTGCATGATTTCTTTGCCAGCGAACGCATTGACCAGGTCTATCTGGCGGCGGCGAAAGTGGGCGGCATTGTTGCCAACAACACCTATCCGGCGGAttt                >  1:96304/1‑150 (MQ=255)                                                                                                   ggAACTGGTATTACGCACCCGCAACGAGCTGAACCTGCTGGACAGCCACGCGGTGCATGATTTCTTTGCCAGCGAACGCATTGACCATGTCTATCTGGCGGCGGCGAAAGTGGGCGGCATTGTTGCCAACAACACCTATCCGGCGGAttt                >  1:4100139/1‑150 (MQ=255)                                                                                                     aaCTGGTATTACGCACCCGCAACGAGCTGAACCTGCTGGACAGCCGCGCGGTGCATGATTTCTTTGCCAGCGAACGCATTGACCAGGTCTATCTGGCGGCGGCGAAAGTGGGCGGCATTGTTGCCAACAACACCTATCCGGCGGATTTCa              <  1:3041214/150‑1 (MQ=255)                                                                                                      aCTGGTATTACGCACCCGCAACGAGCTGAACCCACTGGACAGCCGCGCGGTGCATGATTTCTTTGCCAGCGAACGCATTGACCAGGTCTATCTGGCGGCGGCGAAAGTGGGCGGCATTGTTGCCAACAACACCTATCCGGCGGATTTCAt             <  1:2678840/150‑1 (MQ=255)                                                                                                        tggcaTTACGCACCCGCAACGAGCTGAACCTGCTGGACAGCCGCGCGGTGCATGATTTCTTTGCCAGCGAACGCATTGACCAGGTCTATCTGGCGGCGGCGAAAGTGGGCGGCATTGTTGCCAACAACACCTATCCGGCGGATTTCATCt           >  1:2881161/5‑150 (MQ=255)                                                                                                        tGGTATTACGCACCCGCAACGAGCTGAACCTGCTGGACAGCCGCGCGGTGCATGATTTCTTTGCCAGCGAACGCATTGACCAGGTCTATCTGGCGGCGGCGAAAGTGGGCGGCATTGTTGCCAACAACACCTATCCGGCGGATTTCATCt           <  1:1127591/150‑1 (MQ=255)                                                                                                         ggTATTACGCACCCGCAACGAGCTGAACCTGCTGGACAGCCGCGCGGTGCATGATTTCTTTGCCAGCGAACGCATTGACCAGGTCTATCTGGCGGCGGCGAAAGTGGGCGGCATTGTTGCCAACAACACCTATCCGGCGGATTTCAt             <  1:25239/147‑1 (MQ=255)                                                                                                          gTATTACGCACCCGCAACGAGCTGAACCTGCTGGACAGCCGCGCAGTGCATGATTTCTTTGCCAGCGAACGCATTGACCAGGTCTATCTGGCGGCGGCGAAAGTGGGCGGCATTGTTGCCAACAACACCTATCCGGCGGATTTCATCTAc         >  1:1004680/1‑150 (MQ=255)                                                                                                            aTTACGCACCCGCAACGAGCTGAACCTGCTGGACAGCCGCGCGGTGCATGATTTCTTTGCCAGCGAACGCATTGACCAGGTCTATCTGGCGGCGGCGAAAGTGGGCGGCATTGTTGCCAACAACACCTATCCGGCGGATTTCATCTACCa       <  1:2957084/150‑1 (MQ=255)                                                                                                                    cccGCAACGAGCTGAACCTGCTGGACAGCCGCGCGGTGCATGATTTCTTTGCCAGCGAACGCATTGACCAGGTCTATCTGGCGGCGGCGAAAGTGGGCGGCATTGTTGCCAACAACACCTATCCGGCGGATTTCATCTACCAGAACa  <  1:1573375/147‑1 (MQ=255)                                                                                                                         |                                                                                                                                              GGAGTCATAAGCATGAGTAGACAACGCATTTTTATCGCTGGCCATCGCGGAATGGTCGGTTCTGCCATCAGGCGGCAGCTCGAACAGCGCGGTGATGTGGAACTGGTATTACGCACCCGCGACGAGCTGAACCTGCTGGACAGCCGCGCGGTGCATGATTTCTTTGCCAGCGAACGCATTGACCAGGTCTATCTGGCGGCGGCGAAAGTGGGCGGCATTGTTGCCAACAACACCTATCCGGCGGATTTCATCTACCAGAACA  >  Xen14_65/17130‑17391

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑