BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	Xen14_202	314	T→A	intergenic (–/–)	– / –	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Xen14_202	314	0	T	A	91.2%	1947.3 / 151.2	799	intergenic (–/–)	–/–	–/–
Reads supporting (aligned to +/- strand): ref base T (31/39); new base A (327/400); total (358/441)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 800 total aligned reads displayed.

ACTGTCAGCAACAGCGACATCATCCGGATAAACGCAGTGCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACAGCGCGTATACGATGAAAATCACAAGGTATACGGTGTGCGTAAAGTCTGGCGTCAGTTGTTACGGGAAGGTATCAGAGTGGCCAGATGCACTGTGGCACGTCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCGTTGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  Xen14_202/166‑421

  tGTCAGCAACAGCGACATCATCCTGATAAACGCAGTGCCCGTGCGCAGCGCGATGACTGGCTGAAGAAGGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATc                                                                                                                         >  1:242864/1‑150 (MQ=255)

   gTCAGCAACAGCGACATCATCCTGATAAACGCAGTGCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCa                                                                                                                        <  1:1312365/150‑1 (MQ=255)

   gTCAGCAACAGCGACATCATCCTGATAAACGCAGTGCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCa                                                                                                                        >  1:1204463/1‑150 (MQ=255)

      aGCAACAGCGACATCATCCTGATAAACGCAGTGCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCaggg                                                                                                                     <  1:3322277/150‑3 (MQ=255)

      aGCAACAGCGACATCATCCTGATAAACGCAGTGCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCaggg                                                                                                                     >  1:1913053/1‑148 (MQ=255)

       gtctcaaaagtgaccataagctctcctgaatcaggagagcagattaccccctggatctgatttcaggcgttgggtgtggatcactattgcaccgttcgtgacaGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAatcagggt                                                                                                                    >  1:2277345‑M2/104‑147 (MQ=255)

       gCAACAGCGACATCATCCTGATAAACGCAGTGCCCGTGCGCAGTGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCagggt                                                                                                                    >  1:1480059/1‑147 (MQ=255)

         aaCAGCGACATCATCCTGATAAACGCAGTGCCCTTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCagggtag                                                                                                                  <  1:3405370/150‑6 (MQ=255)

         aaCAGCGACATCATCCTGATAAACGCAGTGCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCagggtag                                                                                                                  <  1:1587562/150‑6 (MQ=255)

         aaCAGCGACATCATCCTGATAAACGCAGTGCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCagggtag                                                                                                                  <  1:655185/150‑6 (MQ=255)

           cAGCGACATCATCCTGATAAACGCAGTGCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCagggtagcc                                                                                                                >  1:1231830/1‑143 (MQ=255)

                aCATCATCCTGATAAACGCAGTGCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATg                                                                                                           >  1:3067994/1‑150 (MQ=255)

                     cataagctctcctgaatcaggagagcagattaccccctggatctgatttcaggcgttgggtgtggatcactattgcaccgttcgtgacaGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCag                                                                                                                       <  1:2327484‑M2/44‑1 (MQ=255)

                     atcCTGATAAACGCAGTGCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACTGTGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATgtacag                                                                                                      <  1:170633/150‑6 (MQ=255)

                     atcCTGATAAACGCAGTGCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATgtacag                                                                                                      <  1:959698/150‑6 (MQ=255)

                     atcCTGATAAACGCAGTGCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATgtacag                                                                                                      >  1:1842550/1‑145 (MQ=255)

                        cGGATAAACGCAGTGCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACAGCGCGTATACGAGGAAAATCACAAGGTATACGGTGTGCGTAAAGTCTGGCGTCAGTTGTTACGGGAAGGTATCAGAGTGGCCAGATGCACTGTgg                                                                                                   <  1:2517678/150‑1 (MQ=255)

                          gATAAACGCAGTGCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGcg                                                                                                 <  1:1805049/150‑2 (MQ=255)

                          gATAAACGCAGTGCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGcg                                                                                                 <  1:578847/150‑2 (MQ=255)

                            tAAACGCAGTGCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGcgcg                                                                                               <  1:1631186/150‑4 (MQ=255)

                            tAAACGCAGTGCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGcgcg                                                                                               >  1:2477984/1‑147 (MQ=255)

                            tAAACGCAGTGCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATAAGGGTAGCCAGATGTACAGTGGcgtg                                                                                               >  1:3854870/1‑147 (MQ=255)

                             aaaCGCAGTGCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGcgcgc                                                                                              >  1:3272241/1‑146 (MQ=255)

                              aaCGCAGTGCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGcgcgcc                                                                                             <  1:3488659/150‑6 (MQ=255)

                              aaCGCAGTGCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGtc                                                                                             >  1:1655411/1‑150 (MQ=255)

                               cctgaatcaggagagcagattaccccctggatctgatttcaggcgttgggtgtggatcactattgcaccgttcgtgacaGGTGTGCGTAAAGTCTGGAGCCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTAcagtggcgcgcct                                                                                            >  1:3045359‑M2/80‑144 (MQ=255)

                                      gCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCg                                                                                     >  1:2185749/1‑150 (MQ=255)

                                      gCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCAGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCg                                                                                     <  1:1881331/150‑1 (MQ=255)

                                      gCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACAGCGCGTATACGATGAAAATCATAAGGTATACGGTGTGCGTAAAGTCTGGCGTCAGTTGTTACGGGAAGGTATCAGAGTGGCCAGATGCACTGTGGCACGTCTCATGGCg                                                                                     <  1:4119772/150‑1 (MQ=255)

                                       cccGTGCGCAGCGCGATGACTGGCTGAAGAAGGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCgg                                                                                    >  1:3003066/1‑150 (MQ=255)

                                       cccGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACAGCGCGTATACGATGAAAATCACAAGGTATACGGTGTGCGTAAAGTCTGGCGTCAGTTGTTACGGGAAGGTATCAGAGTGGCCAGATGCACTGTGGCACGTCTCATGGCgg                                                                                    >  1:2961359/1‑150 (MQ=255)

                                        ccGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGt                                                                                   >  1:1116186/1‑150 (MQ=255)

                                         cGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGtt                                                                                  <  1:2574170/150‑1 (MQ=255)

                                             cgcAGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATgg                                                                              <  1:2049811/150‑1 (MQ=255)

                                                aGCGCGATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGAc                                                                           <  1:1381510/150‑1 (MQ=255)

                                                   gcgATGACTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTg                                                                        <  1:344933/150‑1 (MQ=255)

                                                         aCTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGgtg                                                                  <  1:2354824/150‑1 (MQ=255)

                                                          cTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGgtgt                                                                 >  1:1744513/1‑150 (MQ=255)

                                                          cTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGgtgt                                                                 <  1:1169371/150‑1 (MQ=255)

                                                          cTGGCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGgtgt                                                                 >  1:2616709/1‑150 (MQ=255)

                                                          cTGGCTGAAGAAAGAGATACAGCGCGTATACGATGAAAATCACAAGGTATACGGTGTGCGTAAAGTCTGGCGTCAGTTGTTACGGGAAGGTATCAGAGTGGCCAGATGCACTGTGGCACGTCTCATGGCGGTTATGGGACTTGCCGgtgt                                                                 <  1:3717095/150‑1 (MQ=255)

                                                            ggCTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTtc                                                               >  1:1351530/1‑150 (MQ=255)

                                                             atctgatttcaggcgttgggtgtggatcactattgcaccgttcgtgacaGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTtct                                                              >  1:4197396‑M2/50‑150 (MQ=255)

                                                              cTGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTtctc                                                             >  1:3825039/1‑150 (MQ=255)

                                                              cTGAAGAAAGAGACACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTtctc                                                             >  1:469237/1‑150 (MQ=255)

                                                               tGAAGAAAGAGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTcc                                                            <  1:3650779/150‑1 (MQ=255)

                                                                 aagaaagaGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCgg                                                          <  1:2250233/150‑1 (MQ=25)

                                                                   tttcaggcgttgggtgtggatcactattgcaccgttcgtgacaGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCgggg                                                        <  1:2660544‑M2/107‑1 (MQ=255)

                                                                   gaaagaGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCgggg                                                        >  1:709762/1‑150 (MQ=25)

                                                                     aagaGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTa                                                      <  1:3735566/150‑1 (MQ=25)

                                                                     aagaGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTa                                                      >  1:1086727/1‑150 (MQ=25)

                                                                        ggcgttgggtgtggatcactattgcaccgttcgtgacaGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTaaaa                                                   >  1:4337560‑M2/39‑150 (MQ=255)

                                                                        agaTACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTATGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTaaa                                                    <  1:3899122/149‑1 (MQ=21)

                                                                        agaTACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTaaaa                                                   <  1:3777438/150‑1 (MQ=21)

                                                                           tactgcgcGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAgg                                                <  1:3360404/146‑1 (MQ=21)

                                                                           tACAGCGCGTATACGATGAAAATCACAAGGTATACGGTGTGCGTAAAGTCTGGCGTCAGTTGTTACGGGAAGGTATCAGAGTGGCCAGATGCACTGTGGCACGTCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAgg                                                <  1:2222510/150‑1 (MQ=255)

                                                                           gttgggtgtggatcactattgcaccgttcgtgacaGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGAGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAgg                                                <  1:1488793‑M2/115‑1 (MQ=255)

                                                                             tgggtgtggatcactattgcaccgttcgtgacaGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTc                                              <  1:2306412‑M2/117‑1 (MQ=255)

                                                                             ctgcgcGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTc                                              <  1:2022324/148‑1 (MQ=21)

                                                                              tgcgcGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTcc                                             >  1:630351/2‑150 (MQ=21)

                                                                               gcgcGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCg                                            >  1:1000824/1‑150 (MQ=21)

                                                                                 gcgTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAATCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTa                                          <  1:2386354/150‑1 (MQ=21)

                                                                                  cgTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTAc                                         >  1:415944/1‑150 (MQ=21)

                                                                                   gTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACg                                        <  1:2761538/150‑1 (MQ=21)

                                                                                     gatcactattgcaccgttcgtgacaGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAACCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGAc                                      <  1:1053094‑M2/125‑1 (MQ=255)

                                                                                     gatcactattgcaccgttcatgacaGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGAc                                      <  1:1958810‑M2/125‑1 (MQ=255)

                                                                                     ataCGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGAc                                      <  1:824039/150‑1 (MQ=21)

                                                                                       acgatgggaatcatcaGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCa                                    <  1:1526228/135‑1 (MQ=18)

                                                                                         gatgggaatcatcaGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATc                                  <  1:4234023/137‑1 (MQ=18)

                                                                                         gatgggaatcatcaGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATc                                  >  1:3144149/14‑150 (MQ=18)

                                                                                         gatgggaatcatcaGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATc                                  >  1:503770/14‑150 (MQ=18)

                                                                                         actattgcaccgttcgtgacaGGTGTGCGTAAAGTCTGGAGTCAATTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATc                                  >  1:3101906‑M2/22‑150 (MQ=255)

                                                                                          atgggaatcatcaGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCa                                 <  1:169886/138‑1 (MQ=18)

                                                                                             ttgcaccgttcgtgacaGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGcc                              >  1:3189223‑M2/18‑150 (MQ=255)

                                                                                             ggaatcatcaGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGcc                              <  1:2794731/141‑1 (MQ=18)

                                                                                              gaatcatcaGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGACGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCg                             >  1:2833745/9‑150 (MQ=18)

                                                                                               gcaccgttcgtgacaGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATAGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCgg                            >  1:2114034‑M2/16‑150 (MQ=255)

                                                                                               aatcatcaGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCgg                            >  1:860938/8‑150 (MQ=18)

                                                                                                atcatcaGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGa                           <  1:155320/144‑1 (MQ=18)

                                                                                                  catcaGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGaaa                         >  1:767535/5‑150 (MQ=18)

                                                                                                  catcaGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGaaa                         <  1:3608282/146‑1 (MQ=18)

                                                                                                   atcaGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAg                        <  1:288307/147‑1 (MQ=18)

                                                                                                   atcaGGTATACGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAg                        >  1:3926334/4‑150 (MQ=18)

                                                                                                       ggTATACGGTGTGCGTAAAGTCTGGCGTCAGTGGTTACGGGAAGGTATCAGAGTGGCCAGATGCACTGTGGCACGTCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCGt                    >  1:3570807/1‑150 (MQ=255)

                                                                                                         tataCGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCGTTg                  <  1:2628951/150‑1 (MQ=18)

                                                                                                         tataCGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCGTTg                  <  1:2449599/150‑1 (MQ=18)

                                                                                                         tataCGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCGTTg                  <  1:3232122/150‑1 (MQ=18)

                                                                                                          atgcGGTGTGCGCAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCCCATGGCGGTTATGGGACTTGCCGTTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCGTTGc                 <  1:3954506/147‑1 (MQ=17)

                                                                                                            aCGGTGTGCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGCGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCGTTGccg               >  1:2164964/1‑148 (MQ=17)

                                                                                                                tgtgCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCGTTGccgcagg           >  1:2690805/1‑144 (MQ=17)

                                                                                                                tgtgCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCGTTGccgcagg           <  1:2729828/150‑7 (MQ=17)

                                                                                                                 gtgCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGTGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGNCGGAAAGCCGTTGccgcaggc          <  1:3519761/150‑8 (MQ=17)

                                                                                                                 gtgCGTAAAGTCAGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCGTTGccgcaggc          <  1:2921156/150‑8 (MQ=17)

                                                                                                                  tgCGTAAAGTCTGGCGTCAGTTGTTACGGGAAGGTATCAGAGTGGCCAGATGCACTGTGGCACGTCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCGTTGccgcaggcg         >  1:1271333/1‑142 (MQ=255)

                                                                                                                   gCGTAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCGTTGccgcaggcga        >  1:738052/1‑141 (MQ=17)

                                                                                                                      tAAAGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCGTTGccgcaggcgaccg     <  1:4048230/150‑13 (MQ=14)

                                                                                                                       aaaGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCGTTGccgcaggcgaccgc    >  1:1978594/1‑137 (MQ=14)

                                                                                                                       aaaGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCGTTGccgcaggcgaccgc    >  1:3986246/1‑137 (MQ=14)

                                                                                                                         aGTCTGGAGTCAGTTGTTACGGGAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCGTTGccgcaggcgaccgcgt  <  1:89494/150‑16 (MQ=11)

                                                                                                                                               gAAGGAATCAGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCGTTGccgcaggcg         >  1:4430485/1‑113 (MQ=1)

ACTGTCAGCAACAGCGACATCATCCGGATAAACGCAGTGCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAGAGATACAGCGCGTATACGATGAAAATCACAAGGTATACGGTGTGCGTAAAGTCTGGCGTCAGTTGTTACGGGAAGGTATCAGAGTGGCCAGATGCACTGTGGCACGTCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCGTTGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  Xen14_202/166‑421

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`