BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	Xen14_191	292	T→C	intergenic (–/–)	– / –	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Xen14_191	292	0	T	C	90.6%	2017.6 / 114.0	858	intergenic (–/–)	–/–	–/–
Reads supporting (aligned to +/- strand): ref base T (34/46); new base C (324/451); total (360/498)
Fisher's exact test for biased strand distribution p-value = 9.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 859 total aligned reads displayed.

CCTGGAGACTAAACTTCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCA‑ACGGGCAGTCCGTATGGTTCTGGAAAGTCAGAGCGAATATGACTCACAATGGGCGACAATTTGTTCCATTGCTCCAAAGATTGGCTGTACGCCGGAGACTCTGCGTGTCTGGGTTCGCCAGCATGAGCGGGATACCGGGGGCGGTGATGGAGGGCTCACCACCGCTGAACGTCAGCGTCTGAAAGAGCTGGAACGTGAAAATCGTGAACTGCGCCGCAG  >  Xen14_191/145‑438

   ggAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCA‑GAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCaaa                                                                                                                                               >  1:3410095/1‑150 (MQ=21)

     agaCTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCA‑GAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGa                                                                                                                                             >  1:469779/1‑150 (MQ=21)

      gaCTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCA‑GAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGAt                                                                                                                                            >  1:1357409/1‑150 (MQ=21)

        cTAAACTTCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCA‑ATGGGCAGTCCGTATGGTTCTGGAAAGTCAGAGCGAATATGACTCACAATGGGCGACAATTTGTTCCATTGCTCCAAAGATTg                                                                                                                                          <  1:2584497/150‑1 (MQ=255)

          aaaCTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCGGTCA‑GAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGc                                                                                                                                        <  1:2046075/150‑1 (MQ=21)

          aaaCTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCA‑GAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGc                                                                                                                                        <  1:2962673/150‑1 (MQ=25)

          aaaCTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCA‑GAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGc                                                                                                                                        <  1:4094864/150‑1 (MQ=25)

          aaaCTCCCTGAGAAAGAGGTAAACAGGATGAATAAAAATACTCTTTTTTCCCCCGAAGTCCGTTA‑GAGGGCGATTCGTATGGTTCTGGAAAGGCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGc                                                                                                                                        <  1:3504504/150‑1 (MQ=21)

           aaCTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGAGGGGGA‑TTCGTATGGTTCTGGAAAGTNAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCt                                                                                                                                       >  1:3951219/1‑150 (MQ=25)

           aaCTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCA‑GAGGGCGATTCGTATGGTTCTGGAAAGTCAGGACGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCt                                                                                                                                       <  1:403620/150‑1 (MQ=25)

             ctcccTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCA‑GAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGt                                                                                                                                     >  1:4119302/4‑150 (MQ=25)

                ccTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCA‑ACAGGCAGTCCGTATGGTTCTGGAAAGTCAGAGCGAATATGACTCACAATGGGCGACAATTTGTTCCATTGCTCCAAAGATTGGCTGTACg                                                                                                                                  <  1:2728557/150‑1 (MQ=255)

                 cTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCA‑GAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTAcac                                                                                                                                 >  1:208039/1‑148 (MQ=37)

                 cTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCA‑GAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTAcac                                                                                                                                 >  1:2411707/1‑148 (MQ=37)

                      aaaGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCA‑GAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGgag                                                                                                                            <  1:1113228/150‑1 (MQ=37)

                      aaaGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCA‑ACGGGCAGTCCGTATGGTTCTGGAAAGTCAGAGCGAATATGACTCACAATGGGCGACAATTTGTTCCATTGCTCCAAAGATTGGCTGTACGCCGgag                                                                                                                            >  1:602247/1‑150 (MQ=255)

                       aaGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCA‑GAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGgaga                                                                                                                           >  1:3242696/1‑150 (MQ=37)

                        agagGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCA‑GAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGAc                                                                                                                          >  1:4067944/1‑150 (MQ=37)

                        agagGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCA‑ACGGGCAGTCCGTATGGTTCTGGAAAGTCAGAGCGAATATGACTCACAATGGGCGACAATTTGTTCCATTGCTCCAAAGATTGGCTGTACGCCGGAGAc                                                                                                                          <  1:512337/150‑1 (MQ=255)

                            gTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCA‑GAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTg                                                                                                                      >  1:886537/1‑150 (MQ=255)

                             tAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCA‑ACGGGCAGTCCGTATGGTTCTGGAAAGTCAGAGCGAATATGACTCACAATGGGCGACAATTTGTTCCATTGCTCCAAAGATTGGCTGTACGCCGGAGACTCTGc                                                                                                                     <  1:1484174/150‑1 (MQ=255)

                              aaaCAGGATGACTAAAAATACTCGTTTTTCCCCGGAAGTCCGTCA‑GAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCg                                                                                                                    >  1:3366969/1‑150 (MQ=255)

                              aaaCAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCA‑GAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCg                                                                                                                    >  1:1517817/1‑150 (MQ=255)

                              aaaCAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCA‑GAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCg                                                                                                                    <  1:4426075/150‑1 (MQ=255)

                              aaaCAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCA‑GAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCg                                                                                                                    <  1:1956972/150‑1 (MQ=255)

                                aCAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCA‑GAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCgtg                                                                                                                  <  1:3126543/150‑1 (MQ=255)

                                  aGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCA‑GAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGTTGTACACCGGAGACTCTGCGTGTc                                                                                                                >  1:4014507/1‑150 (MQ=255)

                                    gATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCA‑GAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTg                                                                                                              <  1:2858096/150‑1 (MQ=255)

                                    gATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCA‑GAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTg                                                                                                              <  1:1067690/150‑1 (MQ=255)

                                    gATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCA‑GAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTg                                                                                                              <  1:3090561/150‑1 (MQ=255)

                                      tGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCA‑GAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTggg                                                                                                            <  1:3530920/150‑1 (MQ=255)

                                       gACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCA‑GAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGt                                                                                                           >  1:681393/1‑150 (MQ=255)

                                        aCTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCA‑GAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGta                                                                                                          <  1:2630822/150‑2 (MQ=255)

                                        aCTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCA‑GAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGta                                                                                                          >  1:1639890/1‑149 (MQ=255)

                                           aaaaaTACTCGTTTTTCCCCCGAAGTCCGTCA‑GATGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGc                                                                                                       >  1:3000987/1‑150 (MQ=255)

                                           aaaaaTACTCGTTTTTCCCCCGAAGTCCGTCA‑GAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGc                                                                                                       >  1:100152/1‑150 (MQ=255)

                                            aaaaTACTCGTTTTTCCCCCGAAGTCCGTCA‑GAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACTTTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGcc                                                                                                      <  1:1152262/150‑1 (MQ=255)

                                              aaTACTCGTTTTTCCCCCGAATTCCGTCA‑GAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAg                                                                                                    <  1:4211877/150‑1 (MQ=255)

                                              aaTACTCGTTTTTCCCCCGAAGTCCGTCA‑GAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAg                                                                                                    <  1:1444423/150‑1 (MQ=255)

                                              aaTACTCGTTTTTCCCCCGAAGTCCGTCA‑GAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGCGGGCGGCAATTTGTTCCACTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAg                                                                                                    <  1:1202461/150‑1 (MQ=255)

                                                 aCTCGTTTTTCCCCCGAAGTCCGTCA‑GAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCAt                                                                                                 <  1:1738341/150‑1 (MQ=255)

                                                  cTCGTTTTTCCCCCGAAGTCCGTCA‑GAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCTGAGACTCTGCGTGTCTGGGTACGCCAGCATg                                                                                                <  1:3849421/150‑1 (MQ=255)

                                                    cGTTTTTCCCCCGAAGTCCGTCA‑GAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAg                                                                                              >  1:1658016/1‑150 (MQ=255)

                                                     gTTTTTCCCCCGAAGTCCATCA‑GAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGc                                                                                             >  1:558814/1‑150 (MQ=255)

                                                       ttttCCCCCGAAGTCCGTCA‑GAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCgg                                                                                           <  1:2306238/150‑1 (MQ=255)

                                                        tttCCCCCGAAGTCCGTCA‑GAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCggg                                                                                          <  1:4152942/150‑1 (MQ=255)

                                                        tttCCCCCGAAGTCCGTCA‑GAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCggg                                                                                          <  1:3333355/150‑1 (MQ=255)

                                                            ccccGAAGTCCGTCA‑GATGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTggg                                                                                                            <  1:183326/128‑1 (MQ=255)

                                                            ccccGAAGTCCGTAA‑GAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAATGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACCCTGCGTGTCTGGGTACGCCAGCATGAGCGGGATAc                                                                                      >  1:2780107/1‑150 (MQ=255)

                                                             cccGAAGTCCGTCA‑GAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCGGGATAcc                                                                                     >  1:251177/1‑150 (MQ=255)

                                                             cccGAAGTCCGTCA‑GAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCGGGATAcc                                                                                     >  1:646610/1‑150 (MQ=255)

                                                             cccGAAGTCCGTCA‑GAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCGGGATAcc                                                                                     >  1:2361703/1‑150 (MQ=255)

                                                             cccGAAGTCCGTCA‑GAGGGCGATTCGTATGGTTCTGGAAA‑TCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCGGGATACCg                                                                                    <  1:538523/150‑1 (MQ=255)

                                                                       cgtcagagggcgattcGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCGGGATACCGGAGGCGGt                                                                            <  1:307772/135‑1 (MQ=255)

                                                                       cgtcagagggcgattcGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCGGGATACCGGAGGCGGt                                                                            <  1:3216696/135‑1 (MQ=255)

                                                                          a‑aCGGGCAGTCCGTATGGTTCTGGAAAGTCAGAGCGAATATGACTCACAATGGGCGACAATTTGTTCCATTGCTCCAAAGATTGGCTGTACGCCGGAGACTCTGCGTGTCTGGGTTCGCCAGCATGAGCGGGATACCGGGGGCGGTGATg                                                                        >  1:1875038/1‑150 (MQ=255)

                                                                           agagggcgattcGTATGGTTCTGGAAAGTCAGGGCGAACATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCGGGATACCGGAGGCGGTGATg                                                                        <  1:3669053/139‑1 (MQ=255)

                                                                            gagggcgattcGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCGGGATACCGGAGGCGGTGATgg                                                                       >  1:1494775/11‑150 (MQ=255)

                                                                                 cgattcGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCGGGATACCGGAGGCGGTGATGGCGGGc                                                                  <  1:1036571/145‑1 (MQ=255)

                                                                                 cgattcGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCGGGATACCGGAGGCGGTGATGGCGGGc                                                                  <  1:2345211/145‑1 (MQ=255)

                                                                                 cgattcGTATGGGTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCGGGATACCGGAGGCGGTGATGGCGGGc                                                                  <  1:3281856/145‑1 (MQ=255)

                                                                                    ttcGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCGGGATACCGGAGGCGGTGATGGTGGGCTca                                                               <  1:2610159/148‑1 (MQ=255)

                                                                                     tcGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCGGGATACCGGAGGCGGTGATGGCGGGCTcac                                                              <  1:131966/149‑1 (MQ=255)

                                                                                      cGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCGGGATACCGGAGGCGGTGATGGCGGGCTcacc                                                             <  1:756610/150‑1 (MQ=255)

                                                                                       gTATGGTTCTGGAAAGTCAGAGCGAATATGACTCACAATGGGCGACAATTTGTTCCATTGCTCCAAAGATTGGCTGTACGCCGGAGACTCTGCGTGTCTGGGTTCGCCAGCATGAGCGGGATACCGGGGGCGGTGATGGAGGGCTcacca                                                            >  1:3038667/1‑150 (MQ=255)

                                                                                        tATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGTGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCGGGATACCGGAGGCGGTGATGGCGGGCTcaccac                                                           <  1:1986223/150‑1 (MQ=255)

                                                                                         aTGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCGGGATACCGGAGGCGGTGATGGCGGGCTCaccacc                                                          <  1:925289/150‑1 (MQ=255)

                                                                                          tGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCGGGATACCGGAGGCGGTGATGGCGAGCTCACCACCg                                                         <  1:1770524/150‑1 (MQ=255)

                                                                                           ggTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCGGGATACCGGAGGCGGTGATGGCGGGCTCACCACCGc                                                        <  1:2686113/150‑1 (MQ=255)

                                                                                           ggTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCGGGATACCGGAGGCGGTGATGGCGGGCTCACCACCGc                                                        >  1:1270185/1‑150 (MQ=255)

                                                                                           gattCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCGGGATACCGGAGGCGGTGATGGCGGGCTCACCACCGc                                                        <  1:3772426/148‑1 (MQ=255)

                                                                                              tCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTTGGTACGCCAGCATGAGCGGGATACCGGAGGCGGTGATGGCGGGCTCACCACCGCTGa                                                     <  1:193561/150‑1 (MQ=255)

                                                                                               cTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCGGGATACCGGAGGCGGTGATGGCGGGCTCACCACCGCTGaa                                                    <  1:2122774/150‑1 (MQ=255)

                                                                                                tGGAAAGTCATGGCGAATATGACTCACAATGTGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCGGGATACCGGAGGCGGTGATGGCGGGCTCACCACCGCTGAAc                                                   <  1:3568325/150‑1 (MQ=255)

                                                                                                tGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCGGGATACCGGAGGCGGTGATGGCGGGCTCACCACCGCTGAAc                                                   <  1:807289/150‑1 (MQ=255)

                                                                                                   aaaGTCAGAGCGAATATGACTCACAATGGGCGACAATTTGTTCCATTGCTCCAAAGATTGGCTGTACGCCGGAGACTCTGCGTGTCTGGGTTCGCCAGCATGAGCGGGATACCGGGGGCGGTGATGGAGGGCTCACCACCGCTGAACGTc                                                <  1:2462751/150‑1 (MQ=255)

                                                                                                     aGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCGGGATACCGGAGGCGGTGATGGCGGGCTCACCACCGCTGAACGTCAg                                              <  1:214856/150‑1 (MQ=255)

                                                                                                      gTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCGGGATACCGGAGGCGGTGATGGCGGGCTCACCACCGCTGAACGTCAGc                                             >  1:3705751/1‑150 (MQ=255)

                                                                                                        cagggCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAGAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCGGGATACCGGAGGCGGTGATGGCGGGCTCACCACCGCTGAACGTCAGCGt                                           >  1:2192687/5‑150 (MQ=255)

                                                                                                          gggCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCGGGATACCGGAGGCGGTGATGGCGGGCTCACCACCGc                                                        <  1:1404464/133‑1 (MQ=255)

                                                                                                           ggCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCGGGATACCGGAGGCGGTGATGGCGGGCTCACTACCGCTGAACGTCAGCGTCTg                                        >  1:1574895/2‑150 (MQ=255)

                                                                                                             cGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCGGGATACCGGAGGCGGTGATGGCGGGCTCACCACCGCTGAACGTCAGCGTCTGaa                                      <  1:1687607/150‑1 (MQ=255)

                                                                                                               aaTATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCGGGATACCGGAGGCGGTGATGGCGGGCTCACCACCGCTGAACGTCAGCGTCTGAAag                                    >  1:4189167/1‑150 (MQ=255)

                                                                                                                  atGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCGGGATACCGGAGGCGGTGATGGCGGGCTCACCACCGCTGAACGTCAGCGTCTGAAAGAGc                                 <  1:3644474/150‑1 (MQ=255)

                                                                                                                   tGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCGGGATACCGGAGGCGGTGATGGCGGGCTCACCACCGCTGAACGTCAGCGTCTGAAAGAGCt                                >  1:947482/1‑150 (MQ=255)

                                                                                                                   tGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCGGGATACCGGAGGCGGTGATGGCGGGCTCACCACCGCTGAACGTCAGCGTCTGAAAGAGCt                                <  1:2548030/150‑1 (MQ=255)

                                                                                                                    gACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCGGGATACCGGAGGCGGTGATGGCGGGCTCACCACCGCTGAACGTCAGCGTCTGAAAGAGCTg                               >  1:3920774/1‑150 (MQ=255)

                                                                                                                      cTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCGGGATACCGGAGGCGGTGATGGCGGGCTCACCACCGCTGAACGTCAGCGTCTGAAAGAGCTGGa                             >  1:3158097/1‑150 (MQ=255)

                                                                                                                        cacaGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCGGGATACCGGAGGCGGTGATGGCGGGCTCACCACCGCTGAACGTCAGCGTCTGAAAGAGCTGGAAc                           <  1:2804621/150‑1 (MQ=255)

                                                                                                                              gggCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCGGGATACCGGAGGCGGTGATGGCGGGCTCACCACCGCTGAACGTCAGCGTCTGAAAGAGCTGGAACGTGaaa                     >  1:785881/1‑150 (MQ=255)

                                                                                                                              gggCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCGGGATAACGGAGGCGGTGATGGCGGGCTCACCACCGCTGAACGTCAGCGTCTGAAAGAGCTGGAACGTGaaa                     >  1:4276553/1‑150 (MQ=255)

                                                                                                                               ggcggcAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCGGGATACCGGAGGCGGTGATGGCGGGCTCACCACCGCTGAACGTCAGCGTCTGAAAGAGCTGGAACGTGaaaa                    <  1:1603781/150‑1 (MQ=255)

                                                                                                                               ggcggcAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCGGGATACCGGAGGCGGTGATGGCGGGCTCACCACCGCTGAACGTCAGCGTCTGAAAGAGCTGGAACGTGaaaa                    <  1:2260603/150‑1 (MQ=255)

                                                                                                                                gcggcAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATAAGCGGGATACCGGAGGCGGTGATGGCGGGCTCACCACCGCTGAACGTCAGCGTCTGAAAGAGCTGGAACGTGAAAAt                   <  1:851217/146‑1 (MQ=255)

                                                                                                                                   gcAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCGGGATACCGGAGGCGGTGATGGCGGGCTCACCACCGCTGAACGTCAGCGTCTGAAAGAGCTGGAACGTGAAAATCGt                >  1:3461839/2‑150 (MQ=255)

                                                                                                                                    cAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCGGGATACCGGAGGCGGTGATGGCGGGCTCACCACCGCTGAACGTCAGCGTCTGAAAGAGCTGGAACGTGAAAATCGTg               <  1:2244905/150‑1 (MQ=255)

                                                                                                                                    cAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCGGGATACCGGAGGCGGTGATGGCGGGCTCACCACCGCTGAACGTCAGCGTCTGAAAGAGCTGGAACGTGAAAATCGTg               <  1:3738080/150‑1 (MQ=255)

                                                                                                                                      aTTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCGGGATACCGGAGGCGGTGATGGCGGGCTCACCACCGCTGAACGTCAGCGTCTGAAAGAGCTGGAACGTGAAAATCGTGaa             >  1:2912189/1‑150 (MQ=255)

                                                                                                                                       tttGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAACGGGATACCGGAGGCGGTGATGGCGGGCTCACCACCGCTGAACGTCAGCGTCTGAAAGAGCTGGAACGTGAAAATCGTGAAc            <  1:1237506/150‑1 (MQ=255)

                                                                                                                                               aTTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCGGGATACCGGAGGCGGTGATGGCGGGCTCACCACCGCTGAACGTCAGCGTCTGAAAGAGCTGGAACGTGAAAATCGTGAACTGcgccgc    <  1:722430/150‑1 (MQ=255)

CCTGGAGACTAAACTTCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCA‑ACGGGCAGTCCGTATGGTTCTGGAAAGTCAGAGCGAATATGACTCACAATGGGCGACAATTTGTTCCATTGCTCCAAAGATTGGCTGTACGCCGGAGACTCTGCGTGTCTGGGTTCGCCAGCATGAGCGGGATACCGGGGGCGGTGATGGAGGGCTCACCACCGCTGAACGTCAGCGTCTGAAAGAGCTGGAACGTGAAAATCGTGAACTGCGCCGCAG  >  Xen14_191/145‑438

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`