Predicted mutation
evidence seq id position mutation annotation gene description
RA Xen14_191 229 C→T intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Xen14_1912290CT88.9% 1384.2 / 111.5 674intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (35/40);  new base T (280/318);  total (315/359)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 674 total aligned reads displayed.

TGATTGTATGCCTGCGATCAATGCCAAACGTGTTTACCGGATCATGAACCGCCCCGGGAATCCTGGAGACTAAACTTCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAACGGGCAGTCCGTATGGTTCTGGAAAGTCAGAGCGAATATGACTCACAATGGGCGACAATTTGTTCCATTGCTCCAAAGATTGGCTGTACGCCGGAGACTCTGCGTGTCTGGGTTCGCCAGCATGAGCGGGATACCGGGGGCGGTGATGGAGGGCTCA  >  Xen14_191/84‑377
                                                                                                                                                  |                                                                                                                                                    
tGATTGTATGCCTGCGATCAATGCCAAACGTGTTTACCGGATCATGAACCGCCCCGGGAATCCTGGAGACTAAACTTCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAACGGGCAGTCCGTa                                                                                                                                                  >  1:3599268/1‑150 (MQ=255)
  aTTGTATGCCTGCGATCAATGCCAAACGTGTTTACCGGATCATGAACCGCCCCGGGAATCCTGGAGACTAAACTTCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAACGGGCAGTCCGTATg                                                                                                                                                <  1:2600581/150‑1 (MQ=255)
           cTGCGATCAATGCCAAACGTGTTTACCGGATCATGAACCGCCCCGGGAATCCTGGAGACTAAACTTCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAACGGGCAGTCCGTATGGTTCTGGaa                                                                                                                                       <  1:3308538/150‑1 (MQ=255)
                             gtgtaactgagttaaTGAACCGCCCCGGGAATCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGAc                                                                                                                     <  1:4106994/136‑1 (MQ=14)
                             gccagagcctgcaactGAACCGCCCCGGGAATCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGAc                                                                                                                     <  1:1359492/135‑1 (MQ=14)
                                  tcaatctgcttGAACCGCCCCGGGAATCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGAc                                                                                                                     <  1:1243069/135‑1 (MQ=14)
                                    ttatgacctGAACCGCCCCGGGAATCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTcacagt                                                                                                              >  1:81378/9‑148 (MQ=17)
                                     gagttattGAACCGCCCCGGGAATCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTcacagtg                                                                                                             >  1:1788870/8‑147 (MQ=17)
                                     ccagtattGAACCGCCCCGGGAATCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTcacagtg                                                                                                             >  1:866754/8‑147 (MQ=17)
                                       gATCATGAACCGCCCCGGGNATCCTGGAGACTAAACTTCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAACGGGCAGTCCATATGGTTCTGGAAAGTCAGAGCGAATATGACTCACAATggg                                                                                                           >  1:3126197/1‑150 (MQ=255)
                                        ttactGAACCACCCCGGGAATCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGc                                                                                                          <  1:2535035/146‑1 (MQ=18)
                                        accgtGAACCGCCCCGGGAATCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGc                                                                                                          >  1:1899498/5‑150 (MQ=17)
                                         aactGAACCGCCCCGGGAATCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCg                                                                                                         <  1:1086630/147‑1 (MQ=18)
                                           gtGAACCGCCCCGGGAATCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCggc                                                                                                       <  1:4479704/149‑3 (MQ=18)
                                            tGAACCGCCCCGGGAATCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCggca                                                                                                      >  1:1943355/1‑147 (MQ=18)
                                             gAACCGCCCCGGGAATCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATGTGACTCACAGTGGGCGGCaa                                                                                                     >  1:1852474/1‑150 (MQ=17)
                                             gAACCGCCCCGGGAATCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCaa                                                                                                     >  1:1022692/1‑150 (MQ=18)
                                             gAACCGCCCCGGGAATCCTGGAGACTAAACTCCATGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCaa                                                                                                     >  1:1217147/1‑150 (MQ=18)
                                             gAACCACCCCGGAAATCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCaa                                                                                                     <  1:4168915/150‑1 (MQ=18)
                                                  gCCCCGGGAATCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCATAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGt                                                                                                <  1:2321764/150‑1 (MQ=18)
                                                  gCCCCGGGAATCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGt                                                                                                >  1:1621605/1‑150 (MQ=18)
                                                  gCCCCGGGAATCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGt                                                                                                >  1:502797/1‑150 (MQ=18)
                                                  gCCCCGGGAATCCTGGAGACTAAACTCCCTGAGAAAGAGATAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGt                                                                                                <  1:3823028/150‑1 (MQ=18)
                                                   ccccGGGAATCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTATAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGtt                                                                                               <  1:3228846/150‑1 (MQ=18)
                                                   ccccGGGAATCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCTATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGtt                                                                                               <  1:3053691/150‑1 (MQ=18)
                                                    cccGGGAATCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTc                                                                                              >  1:9704/1‑150 (MQ=18)
                                                      cGGGAATCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCa                                                                                            >  1:3779045/1‑150 (MQ=21)
                                                        ggAATCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTTCCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCAt                                                                                           >  1:3494042/1‑150 (MQ=18)
                                                         gAATCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTg                                                                                         <  1:3247843/150‑1 (MQ=21)
                                                          aaTCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGc                                                                                        <  1:733920/150‑1 (MQ=21)
                                                          aaTCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGc                                                                                        <  1:2909799/150‑1 (MQ=21)
                                                            tCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGccc                                                                                      <  1:63951/150‑3 (MQ=21)
                                                             ccTGGAGACTAAACTTCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAACGGGCAGTCCGTATGGTTCTGGAAAATCAGAGCGAATATGACTCACAATGGGCGACAATTTGTTCCATTGCTcc                                                                                     <  1:1270859/150‑1 (MQ=255)
                                                             ccTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGcccc                                                                                     <  1:3110258/150‑4 (MQ=21)
                                                              cTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTTAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCa                                                                                    >  1:1645077/1‑150 (MQ=21)
                                                              cTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCa                                                                                    >  1:1958106/1‑150 (MQ=21)
                                                              cTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCa                                                                                    >  1:1506134/1‑150 (MQ=21)
                                                               tGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCaa                                                                                   <  1:557290/150‑1 (MQ=21)
                                                                ggAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCaaa                                                                                  <  1:3513297/150‑1 (MQ=21)
                                                                  agaCTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGa                                                                                >  1:469779/1‑150 (MQ=21)
                                                                    aCTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGAtt                                                                              <  1:365788/150‑1 (MQ=25)
                                                                     cTAAACTTCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTT‑‑CCCCCGAAGTCCGTCAACGGGCAGTCCGTATGGTTCTGGAAAGTCAGAGCGAATATGACTCACAATGGGCGACAATTTGTTCCATTGCTCCAAAGATTgg                                                                            >  1:3413707/1‑150 (MQ=255)
                                                                     cTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTg                                                                             >  1:4069228/1‑150 (MQ=25)
                                                                     cTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTg                                                                             >  1:4011996/1‑150 (MQ=25)
                                                                       aaaCTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCTTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGc                                                                           <  1:2798765/150‑1 (MQ=21)
                                                                       aaaCTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGc                                                                           <  1:2962673/150‑1 (MQ=25)
                                                                       aaaCTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGc                                                                           <  1:703075/150‑1 (MQ=25)
                                                                       aaaCTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTC‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGc                                                                           >  1:1048655/1‑150 (MQ=25)
                                                                        aaCTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCt                                                                          <  1:3716201/150‑1 (MQ=25)
                                                                        aaCTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGACGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCt                                                                          <  1:403621/150‑1 (MQ=25)
                                                                         actcccTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTg                                                                         <  1:2173891/146‑1 (MQ=25)
                                                                           tcccTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTa                                                                       >  1:3083145/3‑150 (MQ=25)
                                                                             cccgagaAAGAGGTAAACAGGATGACTAAAAATACTCTTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGcc                                                                                       <  1:2681790/129‑2 (MQ=25)
                                                                             ccTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGAAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTAca                                                                     >  1:1433852/1‑149 (MQ=25)
                                                                             ccTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAACAGGCAGTCCGTATGGTTCTGGAAAGTCAGAGCGAATATGACTCACAATGGGCGACAATTTGTTCCATTGCTCCAAAGATTGGCTGTACg                                                                     <  1:2728557/150‑1 (MQ=255)
                                                                              cTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTAcac                                                                    >  1:2361525/1‑148 (MQ=37)
                                                                              cTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTAcac                                                                    >  1:2411707/1‑148 (MQ=37)
                                                                              cTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTAcac                                                                    >  1:248006/1‑148 (MQ=37)
                                                                                gagaAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCg                                                                  <  1:1179841/150‑1 (MQ=37)
                                                                                 agaAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCgg                                                                 <  1:2126132/150‑1 (MQ=37)
                                                                                    aaGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGgaga                                                              >  1:3369840/1‑150 (MQ=37)
                                                                                    aaGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGgaga                                                              <  1:946351/150‑1 (MQ=37)
                                                                                     agagGTAAACAGGATGACTGAAAATACTCTTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACGGTGGGCGGTGATGTGTTCCATTGACCCAAAGATGGGCTGTACACCGGAGAc                                                             <  1:1575555/150‑1 (MQ=255)
                                                                                      gagGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCa                                                                                    >  1:205900/1‑126 (MQ=25)
                                                                                      gagGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGAct                                                            <  1:3883078/150‑1 (MQ=255)
                                                                                       agGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGgag                                                               <  1:3278792/146‑1 (MQ=255)
                                                                                        ggTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGActct                                                          >  1:3543201/1‑150 (MQ=255)
                                                                                         gTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTg                                                         >  1:886537/1‑150 (MQ=255)
                                                                                           aaaCAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAACGGGCAGTCCGTATGGTTCTGGAAAGTCAGAGCGAATATGACTCACAATGGGCGACAATTTGTTCCATTGCTCCAAAGATTGGCTGTACGCCGGAGACTCTGCg                                                       >  1:3339657/1‑150 (MQ=255)
                                                                                             acaagATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACATTGGGCGGCAATTTGTTCCATTGCACCAAAGATTGGCTGTACACCGGAGACTCTGCgtg                                                     >  1:574675/5‑150 (MQ=255)
                                                                                              cAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGTGCGATTCGTATGGTTCTGGAAAGTCAGGTCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGTTGTACACCGGAGACTCTGCgtgt                                                    <  1:2651162/150‑1 (MQ=255)
                                                                                               aGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGACTGTACACCGGAGACTCTGCGTGTc                                                   <  1:313166/150‑1 (MQ=255)
                                                                                                ggATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCt                                                  <  1:1535963/150‑1 (MQ=255)
                                                                                                 gATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTg                                                 <  1:2858096/150‑1 (MQ=255)
                                                                                                  aTGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTgg                                                >  1:2775468/1‑150 (MQ=255)
                                                                                                      taccggcaccTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGtac                                            <  1:316645/141‑3 (MQ=255)
                                                                                                        aaaaaTACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTAc                                                                      >  1:4347710/1‑122 (MQ=255)
                                                                                                        aaaaaTACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGc                                          >  1:100152/1‑150 (MQ=255)
                                                                                                         aaaaTACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGcc                                         <  1:1492373/150‑1 (MQ=255)
                                                                                                           aaTACTCGTTTTT‑CCCCCGAATTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAg                                       <  1:4211877/150‑1 (MQ=255)
                                                                                                            aTACTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGc                                      <  1:1677097/150‑1 (MQ=255)
                                                                                                              aCTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCAt                                    <  1:1738340/150‑1 (MQ=255)
                                                                                                              aCTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCAt                                    >  1:1451877/1‑150 (MQ=255)
                                                                                                               cTCGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCgt                                                      >  1:4131558/1‑131 (MQ=255)
                                                                                                                 cGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAg                                 >  1:675722/1‑150 (MQ=255)
                                                                                                                 cGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAg                                 >  1:1658016/1‑150 (MQ=255)
                                                                                                                 cGTTTTT‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAg                                 >  1:187311/1‑150 (MQ=255)
                                                                                                                  gTTTTT‑CCCCCGAAGTCCGTCAACGGGCAGTCCGTATGGTTCTGGAAAGTCAGAGCGAATATGACTCACAATGGGCGACAATTTGTTCCATTGCTCCAAAGATTGGCTGTACGCCGGAGACTCTGCg                                                       >  1:1114776/1‑127 (MQ=255)
                                                                                                                    tttt‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCgg                              <  1:2306238/150‑1 (MQ=255)
                                                                                                                     ttt‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGNGTACGCCAGCATGAGCggg                             <  1:3911100/150‑1 (MQ=255)
                                                                                                                     ttt‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCggg                             <  1:1325261/150‑1 (MQ=255)
                                                                                                                     ttt‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCggg                             <  1:771285/150‑1 (MQ=255)
                                                                                                                     ttt‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCggg                             <  1:3017137/150‑1 (MQ=255)
                                                                                                                       t‑CCCCCGAAGTCCGTCAGAGGGCGATTCGTATGGGTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATGGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCGGGAt                           <  1:3957039/150‑1 (MQ=255)
                                                                                                                         cccccGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCgg                              >  1:2272623/1‑146 (MQ=255)
                                                                                                                           cccGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCGGGATAcc                        >  1:2014556/1‑150 (MQ=255)
                                                                                                                           cccGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAAGTCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCGGGATAcc                        >  1:251177/1‑150 (MQ=255)
                                                                                                                           cccGAAGTCCGTCAGAGGGCGATTCGTATGGTTCTGGAAA‑TCAGGGCGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACACCGGAGACTCTGCGTGTCTGGGTACGCCAGCATGAGCGGGATACCg                       <  1:538513/150‑1 (MQ=255)
                                                                                                                                 gTCCGTCAACGGGCAGTCCGTATGGTTCTGCAAAGTCAGAGCGAATATGACTCACAATGGGCGACAATTTGTTCCATTGCTCCANAGATTGGCTGTACGCCGGAGACTCTGCGTGTCTGGGT‑CGCCAGCATGAGCGNGATACCGGGGGCg                 <  1:3745657/150‑1 (MQ=255)
                                                                                                                                             gCAGTTCGTATGGTCATGGAAAGTCAGGGCGAATATGACTCACAATTGGCGACAATTTGTTCCATTGCTCCAAAGATTGGCTGTACGCCGGAGACTCTGCGTGTCTGGGTTCGCCAGCATGAGCGGGATACCGGGGGCGGTGagggaggg      <  1:3695615/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
TGATTGTATGCCTGCGATCAATGCCAAACGTGTTTACCGGATCATGAACCGCCCCGGGAATCCTGGAGACTAAACTTCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTT‑CCCCCGAAGTCCGTCAACGGGCAGTCCGTATGGTTCTGGAAAGTCAGAGCGAATATGACTCACAATGGGCGACAATTTGTTCCATTGCTCCAAAGATTGGCTGTACGCCGGAGACTCTGCGTGTCTGGGTTCGCCAGCATGAGCGGGATACCGGGGGCGGTGATGGAGGGCTCA  >  Xen14_191/84‑377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑