| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | Xen14_17 | 80,371 | G→A | intergenic (‑102/–) | Xen14_02064 ← / – | IS4 family transposase ISEc13/– |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Xen14_17 | 80,371 | 0 | G | A | 96.4% | 120.6 / ‑5.1 | 55 | intergenic (‑102/–) | Xen14_02064/– | IS4 family transposase ISEc13/– |
| Reads supporting (aligned to +/- strand): ref base G (2/0); new base A (22/31); total (24/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.86e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GACAGTGATTACCTGTTTCTGTATCTCCCCCAGCCGTTCGGCCATGCGCTCCGAAGCCTGCTGCCAGCGGTAACTTTCTTTTTCTTCACAGGGACGTTCTTTTCGCTGGTGCTTAACACCGTAGGTGTCCGTGGCACGACTCCAGCGCTGCTGTTCGATAAGACCG‑‑‑‑‑‑‑‑‑‑‑‑ > Xen14_17/80225‑80390 | gacagTGATTACCTGTTTCTGTATCTCCCCCAGCCGTTCGGCCATGCGCTCCGAAGCCTGCTGCCAGCGGTAACTTTCTTTTTCTTCACAGGGACGTTCTTTTCGCTGGTGCTTAACACCGTAGGTGTCCGTGGCACGACTCCAGCactg < 1:1279599/150‑1 (MQ=25)gacagTGATTACCTGTTTCTGTATCTCCCCCAGCCGTTCGGCCATGCGCTCCGAAGCCTGCTGCCAGCGGTAACTTTCTTTTTCTTCACAGGGACGTTCTTTTCGCTGGTGCTTAACACCGTAGGTGTCCGTGGCACGACTCCAGCactg < 1:1279604/150‑1 (MQ=25)gacagTGATTACCTGTTTCTGTATCTCCCCCAGCCGTTCGGCCATGCGCTCCGAAGCCTGCTGCCAGCGGTAACTTTCTTTTTCTTCACAGGGACGTTCTTTTCGCTGGTGCTTAACACCGTAGGTGTCCGTGGCACGACTCCAGCactg < 1:1279607/150‑1 (MQ=25) acagTGATTACCTGTTTCTGTATCTCCCCCAGCCGTTCGGCCATGCGCTCCGAAGCCTGCTGCCAGCGGTAACTTTCTTTTTCTTCACAGGGACGTTCTTTTCGCTGGTGCTTAACACCGTAGGTGTCCGTGGCACGACTCCAGCactgc < 1:3586832/150‑1 (MQ=25) agTGATTACCTGTTTCTGTATCTCCCCCAGCCGTTCGGCCATGCGCTCCGAAGCCTGCTGCCAGCGGTAACTTTCTTTTTCTTCACAGGGACGTTCTTTTCGCTGGTGCTTAACACCGTAGGTGTCCGTGGCACGACTCCAGCActgctg < 1:3335607/150‑1 (MQ=25) agTGATTACCTGTTTCTGTATCTCCCCCAGCCGTTCGGCCATGCGCTCCGAAGCCTGCTGCCAGCGGTAACTTTCTTTTTCTTCACAGGGACGTTCTTTTCGCTGGTGCTCAACACCGTAGGTGTCCGTGGCACGACTCCAGCActgctg < 1:11028/150‑1 (MQ=255) agTGATTACCTGTTTCTGTATCTCCCCCAGCCGTTCGGCCATGCGCTCCGAAGCCTGCTGCCAGCGGTAACTTTCTTTTTCTTCACAGGGACGTTCTTTTCGCTGGTGCTCAACACCGTAGGTGTCCGTGGCACGACTCCAGCActgctg < 1:11029/150‑1 (MQ=255) gATTACCTGTTTCTGTATCTCCCCCAGCCGTTCGGCCATGCGCTCCGAAGCCTGCTGCCAGCGGTAACTTTCTTTTTCTTCACAGGGACGTTCTTTTCGCTGGTGCTTAACACCGTAGGTGTCCGTGGCACGACTCCAGCACTGCTGTTc < 1:633279/150‑1 (MQ=25) gATTACCTGTTTCTGTATCTCCCCCAGCCGTTCGGCCATGCGCTCCGAAGCCTGCTGCCAGCGGTAACTTTCTTTTTCTTCACAGGGACGTTCTTTTCGCTGGTGCTTAACACCGTAGGTGTCCGTGGCACGACTCCAGCACTGCTGTTc < 1:1898243/150‑1 (MQ=25) gATTACCTGTTTCTGTATCTCCCCCAGCCGTTCGGCCATGCGCTCCGAAGCCTGCTGCCAGCGGTAACTTTCTTTTTCTTCACAGGGACGTTCTTTTCGCTGGTGCTTAACACCGTAGGTGTCCGTGGCACGACTCCAGCACTGCTGTTc < 1:1898246/150‑1 (MQ=25) gATTACCTGTTTCTGTATCTCCCCCAGCCGTTCGGCCATGCGCTCCGAAGCATGCTGCCAGCGGTAACTTTCTTTTTCTTCACAGGGACGTTCTTTTCGCTGGTGCTTAACACCGTAGGTGTCCGTGGCACGACTCCAGCACTGCTGTTc > 1:3869789/1‑150 (MQ=255) aTTACCTGTTTCTGTATCTCCCCCAGCCGTTCGGCCATGCGCTCCGAAGCCTGCTGCCAGCGGTAACTTTCTTTTTCTTCACAGGGACGTTCTTTTCGCTGGTGCTTAACACCGTAGGTGTCCGTGGCACGACTCCAGCACTGCTGTTCg < 1:2264641/150‑1 (MQ=25) ttACCTGTTTCTGTATCTCCCCCAGCCGTTCGGCCATGCGCTCCGAAGCCTGCTGCCAGCGGTAACTTTCTTTTTCTTCACAGGGACGTTCTTTTCGCTGGTGCTTAACACCGTAGGTGTCCGTGGCACGACTCCAGCACTGCTGTTCGa < 1:3839702/150‑1 (MQ=25) ttACCTGTTTCTGTATCTCCCCCAGCCGTTCGGCCATGCGCTCCGAAGCCTGCTGCCAGCGGTAACTTTCTTTTTCTTCACAGGGACGTTCTTTTCGCTGGTGCTTAACACCGTAGGTGTCCGTGGCACGACTCCAGCACTGCTGTTCGa < 1:2912262/150‑1 (MQ=25) tACCTGTTTCTGTATCTCCCCCAGCCGTTCGGCCATGCGCTCCGAAGCCTGCTGCCAGCGGTAACTTTCTTTTTCTTCACAGGGACGTTCTTTTCGCTGGTGCTTAACACCGTAGGTGTCCGTGGCATGACTCCAGCACTGCTGTTCGAt < 1:2401140/150‑1 (MQ=255) tACCTGTTTCTGTATCTCCCCCAGCCGTTAGGCCATGCGCTCCGAAGCCTGCTGCCAGCGGTAACTTTCTTTTTCTTCACAGGGACGTTCTTTTCGCTGGTGCTTAACACCGTAGGTGTCCGTGGCACGACTCCAGCACTGCTGTTCGAt < 1:2902897/150‑1 (MQ=255) aCCTGTTTCTGTATCTCCCCCAGCCGTTCGGCCATGCGCTCCGAAGCCTGCTGCCAGCGGTAACTTTCTTTTTCTTCACAGGGACGTTCTTTTCGCTGGTGCTTAACACCGTAGGTGTCCGTGGCACGACTCCAGCACTGCTGTTCGATa < 1:1043831/150‑1 (MQ=25) aCCTGTTTCTGTATCTCCCCCAGCCGTTCGGCCATGCGCTCCGAAGCCTGCTGCCAGCGGTAACTTTCTTTTTCTTCACAGGGACGTTCTTTTCGCTGGTGCTTAACACCGTAGGTGTCCGTGGCACGACTCCAGCACTGCTGTTCGATa > 1:2037878/1‑150 (MQ=25) aCCTGTTTCTGTATCTCCCCCAGCCGTTCGGCCATGCGCTCCGAAGCCTGCTGCCAGCGGTAACTTTCTTTTTCTTCACAGGGACGTTCTTTTCGCTGGTGCTTAACACCGTAGGTGTCCGTGGCACGACTCCAGCACTGCTGTTCGATa < 1:3188744/150‑1 (MQ=25) aCCTGTTTCTGTATCTCCCCCAGCCGTTCGGCCATGCGCTCCGAAGCCTGCTGCCAGCGGTAACTTTCTTTTTCTTCACAGGGACGTTCTTTTCGCTGGTGCTTAACACCGTAGGTGTCCGTGGCACGACTCCAGCACTGCTGTTCGATa < 1:3568447/150‑1 (MQ=25) aCCTGTTTCTGTATCTCCCCCAGCCGTTCGGCCATGCGCTCCGAAGCCTGCTGCCAGCGGTAACTTTCTTTTTCTTCACAGGGACGTTCTTTTCGCTGGTGCTTAACACCGTAGGTGTCCGTGGCACGACTCCAGCACTGCTGTTCGATa < 1:3821956/150‑1 (MQ=25) aCCTGTTTCTGTATCTCCCCCAGCCGTTCGGCCATGCGCTCCGAAGCCTGCTGCCAGCGGTAACTTTCTTTTTCTTCACAGGGACGTTCTTTTCGCTGGTGCTTAACACCGTAGGTGTCCGTGGCACGACTCCAGCACTGCTGTTCGATa < 1:4374380/150‑1 (MQ=25) aCCTGTTTCTGTATCTCCCCCAGCCGTTCGGCCATGCGCTCCGAAGCCTGCTGCCAGCGGTAACTTTCTTTTTCTTCACAGGGACGTTCTTTTCGCTGGTGCTTAACACCGTAGGTGTCCGTGGCACGACTCCAGCACTGCTGTTCGANa < 1:2884354/150‑1 (MQ=25) cccgTTTCTGTATCTCCCCCCGCCGTTCGTCCATGCGCTCCGAAGCCTGCTGCCAGCGGTAACTTTCTTTCTCTTCACAGGGAAGTTCTTTTCGCTGGTGCTTAACACAGTAGGTGTCCGTGGTACGAATCCAGCGCTGCTGTTCGATaa > 1:2762985/4‑150 (MQ=11) ccTGTTTCTGTATCTCCCCCAGCCGTTCGGCCATGCGCTCCGAAGTCTGCTGCCAGCGGTAACTTTCTTTTTCTTCACATGGACGTTCTTTTCGCTGGTGCTTAACACCGTAGGTGTCCGTGGCACGACTCCAGCACTGCTGTTCGATa < 1:2926253/149‑1 (MQ=255) ccTGTTTCTGTATCTCCCCCAGCCGTTCGGCCATGCGCTCCGAAGCCTGCTGCCAGCGGTAACTTTCTTTTTCTTCACAGGGACGTTCTTTTCGCTGGTGCTTAACACCGTAGGTGTCCGTGGCACGACTCCAGCACTGCTGTTCGATaa > 1:1663735/1‑150 (MQ=25) ccTGTTTCTGTATCTCCCCCAGCCGTTCGGCCATGCGCTCCGAAGCCTGCTGCCAGCGGTAACTTTCTTTTTCTTCACAGGGACGTTCTTTTCGCTGGTGCTTAACACCGTAGGTGTCCGTGGCACGACTCCAGCACTGCTGTTCGATaa > 1:2277225/1‑150 (MQ=25) ccTGTTTCTGTATCTCCCCCAGCCGTTCGGCCATGCGCTCCGAAGCCTGCTGCCAGCGGTAACTTTCTTTTTCTTCACAGGGACGTTCTTTTCGCTGGTGCTTAACACCGTAGGTGTCCGTGGCACGACTCCAGCACTGCTGTTCGATaa > 1:2277224/1‑150 (MQ=25) ccTGTTTCTGTATCTCCCCCAGCCGTTCGGCCATGCGCTCCGAAGCCTGCTGCCAGCGGTAACTTTCTTTTTCTTCACAGGGACGTTCTTTTCGCTGGTGCTTAACACCGTAGGTGTCCGTGGCACGACTCCAGCACTGCTGTTCGATaa > 1:3149561/1‑150 (MQ=25) cTGTTTCTGTATCTCCCCCAGCCGTTCGGCCATGCGCTCCGAAGCCTGCTGCCAGCGGTAACTTTCTTTTTCTTCACAGGGACGTTCTTTTCGCTGGTGCTTAACACCGTAGGTGTCCGTGGCACGACTCCAGCACTGCTGTTCGATAAg < 1:460730/150‑1 (MQ=21) cTGTTTCTGTATCTCCCCCAGCCGTTCGGCCATGCGCTCCGAAGCCTGCTGCCAGCGGTAACTTTCTTTTTCTTCACAGGGACGTTCTTTTCGCTGGTGCTTAACACCGTAGGTGTCCGTGGCACGACTCCAGCACTGCTGTTCGATAAg < 1:927154/150‑1 (MQ=21) cTGTTTCTGTATCTCCCCCAGCCGTTCGGCCATGCGCTCCGAAGCCTGCTGCCAGCGGTAACTTTCTTTTTCTTCACAGGGACGTTCTTTTCGCTGGTGCTTAACACCGTAGGTGTCCGTGGCACGACTCCAGCACTGCTGTTCGATAAg < 1:2359379/150‑1 (MQ=21) cTGTTTCTGTATCTCCCCCAGCCGTTCGGCCATGCGCTCCGAAGCCTGCTGCCAGCGGTAACTTTCTTTTTCTTCACAGGGACGTTCTTTTCGCTGGTGCTTAACACCGTAGGTGTCCGTGGCACGACTCCAGCACTGCTGTTCGATAAg < 1:2198237/150‑1 (MQ=21) tGTTTCTGTATCTCCCCCAGCCGTTCGGCCATGCGCTCCGAAGCCTGCTGCCAGTGGTAACTTTCTTTTTCTTCACAGGGACGTTCTCTTCGCTGGTGCTTAACACCGTAGGTGTCCGTGGCACGACTCCAGCACTGCTGTTCGATAAGa > 1:424006/1‑150 (MQ=255) tGTTTCTGTATCTCCCCCAGCCGTTCGGCCATGCGCTCCGAAGCCTGCTGCCAGTGGTAACTTTCTTTTTCTTCACAGGGACGTTCTCTTCGCTGGTGCTTAACACCGTAGGTGTCCGTGGCACGACTCCAGCACTGCTGTTCGATAAGa > 1:424004/1‑150 (MQ=255) tGTTTCTGTATCTCCCCCAGCCGTTCGGCCATGCGCTCCGAAGCCTGCTGCCAGCGGTAACTTTCTTTTTCTTCACAGGGACGTTCTTTTCGCTGGTGCTTAACACCGTAGGTGTCTGTGGCACGACTCCAGCACTGCTGTTCGATAAGa > 1:1672004/1‑150 (MQ=16) tGTTTCTGTATCTCCCCCAGCCGTTCGGCCATGCGCTCCGAAGCCTGCTGCCAGCGGTAACTTTCTTTTTCTTCACAGGGACGTTCTTTTCGCTGGTGCTTAACACCGTAGGTGTCCGTGGCACGACTCCAGCACTGCTGTTCGATAAGa > 1:2984807/1‑150 (MQ=18) tGTTTCTGTATCTCCCCCAGCCGTTCGGCCATGCGCTCCGAAGCCTGCTGCCAGCGGTAACTTTCTTTTTCTTCACAGGGACGTTCTTTTCGCTGGTGCTTAACACCGTAGGTGTCCGTGGCACGACTCCAGCACTGCTGTTCGATAAGa > 1:666422/1‑150 (MQ=18) tttCTGTATCTCCCCCAGCCGTTCGGCCATGCGCTCCGAAGCCTGCTGCCAGCGGTAACTTTCTTTTTCTTCACAGGGACGTTCTTTTCGCTGGTGCTTAACACCGTAGGTGTCCGTGGCACGACTCCAGCACTGCTGTTCGATAAGatc < 1:682304/150‑3 (MQ=17) tttCTGTATCTCCCCCAGCCGTTCGGCCATGCGCTCCGAAGCCTGCTGCCAGCGGTAACTTTCTTTTTCTTCACAGGGACGTTCTTTTCGCTGGTGCTTAACACCGTAGGTGTCCGTGGCACGACTCCAGCACTGCTGTTCGATAAGatc < 1:2425967/150‑3 (MQ=17) tCTGTATCTCCCCCAGCCGTTCGGCCATGCGCTCCGAAGCCTGCTGCCAGCGGTAACTTTCTTTTTCTTCACAGGGACGTTCTTTTCGCTGGTGCTTAACACCGTAGGTGTCCGTGGCACGACTCCAGCACTGCTGTTCGATAAGatcga > 1:541614/1‑146 (MQ=12) tCTGTATCTCCCCCAGCCGTTCGGCCATGCACTCCGAAGCCTGCTGCCAGCGGTAACTTTCTTTTTCTTCACAGGGACGTTCTTTTCGCTGGTGCTTAACACCGTAGGTGTCCGTGGCACGACTCCAGCACTGCTGTTCGATAAGatcga > 1:3045393/1‑146 (MQ=255) gTATCTCCCCCAGCCGTTCGGCCATGCGCTCCGAAGCCTGCTGCCAGCGGTAACTCTCTTTTTCTTCACAGGGACGTTCTTTTCGCTGGTGCTTAACACCGTAGGTGTCCGTGGCACGACTCCAGCACTGCTGTTCGATAAGatcgactg < 1:3277284/150‑8 (MQ=11) gTATATCCCCCAGCCGTTCGGCCATGCGCTCCGAAGCCTGCTGCCAGCGGTAACTTTCTTTTTCTTCACAGGGACGTTCTTTTCGCTGGTGCTTAACACCGTAGGTGTCCGTGGCACGACTCCAGCACTGCTGTTCGATAAGatcgactg > 1:281141/1‑143 (MQ=11) aTCTCCCCCAGCCGTTCGGCCATGCGCTCCGAAGCCTGCTGCCAGCGGTAGCTTTCTTTTTCTTCACAGGGACGTTCTTTTCGCTGGTGCTTAACACCGTAGGTGTCCGTGGCACGACTCCAGCACTGCTGTTCGATAAGatcgactggc > 1:1046136/1‑141 (MQ=11) aTCTCCCCCAGCCGTTCGGCCATGCGCTCCGAAGCCTGCTGCCAGCGGTAACTTTCTTTTTCTTCACAGGGACGTTCTTTTCGCTGGTGCTTAACACCGTAGGTGTCCGTGGCACGACTCCAGCACTGCTGTTCGATAAGatcgactggc > 1:684926/1‑141 (MQ=255) tcCCCCAGCCGTTCGGCCATGCGCTCCGAAGCCTGCTGCCAGCGGTAACTTTCTTTTTCTTCACAGGGACGTTCTTTTCGCTGGTGCTTAACACCGTAGGTGTCCGTGGCACGACTCCAGCACTGCTGTTCGATAAGatcgactggcagg < 1:1577470/150‑13 (MQ=11) tcCCCCAGCCGTTCGGCCATGCGCTCCGAAGCCTGCTGCCAGCGGTAACTTTCTTTTTCTTCACAGGGACGTTCTTTTCGCTGGTGCTTAACACCGTAGGTGTCCGTGGCACGACTCCAGCACTGCTGTTCGATAAGatcgactggcagg < 1:1577468/150‑13 (MQ=11) cccccAGCCGTTCGGCCATGCGCTCCGAAGCCTGCTGCCAGCGGTAACTTTCTTTTTCTTCACAGGGACGTTCTTTTCGCTGGTGCTTAACACCGTAGGTGTCCGTGGCACGACTCCAGCACTGCTGTTCGATAAGatcgactggcaggg > 1:3823262/1‑137 (MQ=11) ccccAGCCGTTCGGCCATGCGCTCCGAAGCCTGCTGCCAGTGGTAACTTTCTTTTTCTTCACAGGGACGTTCTTTTCGCTTTTGCTTAACACCATAGGTGTCCGTGGCACGACTCCAGGGCTGCTGTTTGATAAGACCgactggcagggt > 1:2182947/1‑139 (MQ=11) ccccAGCCGTTCGGCCATGCGCTCCGAAGCCTGCTGCCAGCGGTAACTTTCTTTTTCTTCACAGGGACGTTCTTTTCGCTGGTGCTTAACACCGTAGGTGTCCGTGGCACGACTCCAGCACTGCTGTTCGATa > 1:4085759/1‑133 (MQ=18) ccccAGCCGTTCGGCCATGCGCTCCGAAGCCTGCTGCCAGCGGTAACTTTCTTTTTCTTCACAGGGACGTTCTTTTCGCTGGTGCTTAACACCGTAGGTGTCCGTGGCACGACTCCAGCACTGCTGTTCGATAAGatcgactggcagggc > 1:2276874/1‑136 (MQ=11) cccAGCCGTTCGGCCATGCGCTCCGAAGCCTGCTGCCAGTGGTAACTTTCTTTTTCTTCACAGGGACGTTCTTTTCGCTGGTGCTTAACACCGTAGGTGTCCGTGGCACGACTCCAGCACTGCTGTTCGATAAGatcgactggcagggcg > 1:2728058/1‑135 (MQ=11) cccAGCCGTTCGGCCATGCGCTCCGAAGCCTGCTGCCAGCGGTAACTTTCTTTTTCTTCACAGGGACGTTCTTTTCGCTGGTGCTTAACACCGTAGGTGTCCGTGGCACGACTCCAGCACTGCTGTTCGATaa < 1:3589814/133‑1 (MQ=18) cccAGCCGTTCGGCCATGCGCTCCGAAGCCTGCTGCCAGCGGTAACTTTCTTTTTCTTCACAGGGACGTTCTTTTCGCTGGTGCTTAACACCGTAGGTGTCCGTGGCACGACTCCAGCACTGCTGTTCGATAAGatcgactggcagggcg < 1:2361203/150‑16 (MQ=11) cccAGCCGTTCGGCCATGCGCTCCGAAGCCTGCTGCCAGCGGTAACTTTCTTTTTCTTCACAGGGACGTTCTTTTCGCTGGTGCTTAACACCGTAGGTGTCCGTGGCACGACTCCAGCACTGCTGTTCGATAAGatcgactggcagggcg > 1:3073534/1‑135 (MQ=11) cccAGCCGTTCGGCCATGCGCTCCGAAGCCTGCTGCCAGCGGTAACTTTCTTTTTCTTCACAGGGACGTTCTTTTCGCTGGTGCTTAACACCGTAGGTGTCCGTGGCACGACTCCAGCACTGCTGTTCGATAAGatcgactggcagggcc < 1:3627304/150‑16 (MQ=11) gTTCGGCCATGCGCTCCGAAGCCTGCTGCCAGCGGTAACTTTCTTTTTCTTCACAGGGACGTTCTTTTCGCTGGTGCTTAACACCGTAGGTGTCCGTGGCACGACTCCAGCACTGCTGTTCGa > 1:3699940/1‑123 (MQ=18) gcCAGCGGTAACTTTCTTTTTCTTCACAGGGACGTTCTTTTCGCTGGTGCTTAACACCGTAGGTGTCCGTGGCACGACTCCAGCACTGCTGTTCGATAAGatcgactgg < 1:2730489/109‑9 (MQ=11) | GACAGTGATTACCTGTTTCTGTATCTCCCCCAGCCGTTCGGCCATGCGCTCCGAAGCCTGCTGCCAGCGGTAACTTTCTTTTTCTTCACAGGGACGTTCTTTTCGCTGGTGCTTAACACCGTAGGTGTCCGTGGCACGACTCCAGCGCTGCTGTTCGATAAGACCG‑‑‑‑‑‑‑‑‑‑‑‑ > Xen14_17/80225‑80390 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |