BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	Xen14_143	700	C→G	intergenic (‑394/–)	Xen14_04759 ← / –	hypothetical protein/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Xen14_143	700	0	C	G	87.0%	3503.3 / inf	1401	intergenic (‑394/–)	Xen14_04759/–	hypothetical protein/–
Reads supporting (aligned to +/- strand): ref base C (95/86); new base G (621/592); total (720/681)
Fisher's exact test for biased strand distribution p-value = 7.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1412 total aligned reads displayed.

GATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACACCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGACAGGG‑TGTCGAACTGAGCCGTGCATTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACGCCGCTGAATGATGCCCTGTACCGTTATGTGATGAATACCCGCAAGCTTCACACTGACGACACACCGGTAAAGGTACTGGCACCGGACCTGAAAAAGACGAAAACAGGGCGCATCTGGACGTATGTCCGGGATGATCGCAATGCGGGTTCGTCA  >  Xen14_143/554‑849

  ttACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAat                                                                                                                                                  <  1:3888588/150‑1 (MQ=255)

  ttACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACACCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGACAGGG‑TGTCGAACTGAGCCGTGCATTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACGCCGCTGAat                                                                                                                                                  <  1:3069364/150‑1 (MQ=255)

    aCTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAatga                                                                                                                                                >  1:837500/1‑150 (MQ=255)

    aCTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAatga                                                                                                                                                <  1:3394336/150‑1 (MQ=255)

         cccGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGccc                                                                                                                                           >  1:1665390/1‑150 (MQ=255)

         cccGCGTGTTAACGGGAAAATACTGCGAACACCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGACAGGG‑TGTCGAACTGAGCCGTGCATTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACGCCGCTGAATGATGccc                                                                                                                                           >  1:4202691/1‑150 (MQ=255)

          ccGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAANTGAGCCGGGCCTTACTCTTCAACTGGGTTGAAGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCt                                                                                                                                          >  1:3762032/1‑150 (MQ=255)

          ccGCGTGTTAACGGGAAAATACTGCGAACACCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGACAGGG‑TGTCGAACTGAGCCGTGCATTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACGCCGCTGAATGATGc                                                                                                                                             <  1:2242399/147‑1 (MQ=255)

             cgTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTa                                                                                                                                       <  1:1121317/150‑1 (MQ=255)

              gtgtTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTAc                                                                                                                                      <  1:2714780/150‑1 (MQ=255)

                gtTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGTCCTGTACCg                                                                                                                                    >  1:589795/1‑150 (MQ=255)

                gtTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCg                                                                                                                                    <  1:3118852/150‑1 (MQ=255)

                gtTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCg                                                                                                                                    <  1:2760435/150‑1 (MQ=255)

                 ttAACGGGAAAATNCTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGt                                                                                                                                   >  1:2435516/1‑150 (MQ=255)

                 ttAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGt                                                                                                                                   >  1:417268/1‑150 (MQ=255)

                  taaagggAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGtt                                                                                                                                  >  1:3993285/5‑150 (MQ=255)

                  tAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGtt                                                                                                                                  >  1:810446/1‑150 (MQ=255)

                   aaCGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCTGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTa                                                                                                                                 <  1:1746591/150‑1 (MQ=255)

                    aCGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTAt                                                                                                                                >  1:109313/1‑150 (MQ=255)

                     cGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACCGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTAtg                                                                                                                               >  1:2499724/1‑150 (MQ=255)

                        gAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTAtgtaa                                                                                                                            >  1:3163558/1‑148 (MQ=255)

                         aaaaTACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTAtgtaat                                                                                                                           <  1:465366/150‑4 (MQ=255)

                         aaaaTACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTAtgtaat                                                                                                                           <  1:1792431/150‑4 (MQ=255)

                          aaaTACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGtaatg                                                                                                                          <  1:4220030/150‑1 (MQ=255)

                           aaTACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGtaatga                                                                                                                         >  1:3546130/1‑150 (MQ=255)

                            acaCTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCG‑CAGGGGTGTCGAACTGAGCCGGGCCTTACTCTCCCACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGaa                                                                                                                        >  1:2635533/3‑150 (MQ=255)

                            aTACTGCGAACATCTGCCACTGTATTGTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGaa                                                                                                                        <  1:2215475/150‑1 (MQ=255)

                             tactacGAACACCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGACAGGG‑TGTCGAACTGAGCCGTGCATTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACGCCGCTGAATGATGCCCTGTACCGTTATGTGATGAAt                                                                                                                       >  1:3608750/1‑150 (MQ=255)

                               cTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGaacac                                                                                                                     >  1:2376707/1‑147 (MQ=255)

                               cTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATAc                                                                                                                     >  1:4025074/1‑150 (MQ=255)

                                    aaCATCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCa                                                                                                                >  1:4106891/1‑150 (MQ=255)

                                     acacCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGACAGGG‑TGTCGAACTGAGCCGTGCATTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACGCCGCTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCaa                                                                                                               >  1:1488468/1‑150 (MQ=255)

                                        tcTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAACACCCGCAAggt                                                                                                            >  1:202608/2‑148 (MQ=255)

                                         cTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAggtt                                                                                                           >  1:439794/1‑147 (MQ=255)

                                         cTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAggtt                                                                                                           <  1:898100/150‑4 (MQ=255)

                                            ccACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTGCCGTTATGTAATGAATACCCGCAAGGTTcac                                                                                                        <  1:1357110/150‑1 (MQ=255)

                                              aCTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAg                                                                                                              <  1:3294302/142‑1 (MQ=255)

                                              aCTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCacac                                                                                                      >  1:742959/1‑150 (MQ=255)

                                                tGTATCGTCAGAGTGAAATCTTTGCCCGACAGGG‑TGTCGAACTGAGCCGTGCATTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACGCCGCTGAATGATGCCCTGTACCGTTATGTGATGAATACCCGCAAGCTTCACACTg                                                                                                    <  1:1708873/150‑1 (MQ=255)

                                                   aTCGTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTgatg                                                                                                 <  1:2104092/150‑3 (MQ=255)

                                                   aTCGTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAACACCCGCAAGGTTCACACTgatg                                                                                                 >  1:3427240/1‑148 (MQ=255)

                                                      gTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGTCCTGTACCGTTATGTAATGAATACCCGCAAGGTTc                                                                                                          >  1:4144783/1‑138 (MQ=255)

                                                      gTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTACTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGaca                                                                                              >  1:2992104/1‑150 (MQ=255)

                                                       tCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGacac                                                                                             >  1:3253443/1‑150 (MQ=255)

                                                         agagTGACATCTTTGCCCGCCAGGG‑CGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGacacac                                                                                           >  1:3033020/1‑150 (MQ=255)

                                                             tGAAATCTTTGCCAGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGt                                                                                       <  1:2808732/150‑1 (MQ=255)

                                                              gAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTa                                                                                      >  1:2192540/1‑150 (MQ=255)

                                                                aaTCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTaaa                                                                                    >  1:131527/1‑150 (MQ=255)

                                                                 aTCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAg                                                                                   >  1:2851403/1‑150 (MQ=255)

                                                                    tttGCCCGACAGGG‑TGTCGAACTGAGCCGTGCATTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACGCCGCTGAATGATGCCCTGTACCGTTATGTGATGAATACCCGCAAGCTTCACACTGACGACACACCGGTAAAGGTa                                                                                >  1:3568250/1‑150 (MQ=255)

                                                                       gCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTg                                                                             <  1:289885/150‑1 (MQ=255)

                                                                       gCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTg                                                                             >  1:1285319/1‑150 (MQ=255)

                                                                       gCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCAGTAAAGGTACTg                                                                             >  1:3518899/1‑150 (MQ=255)

                                                                       gCCCGACAGGA‑TGTCGAACTGAGCCGTGCATTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTACTGACGCCGCTGAATGATGCCCTGTACCGTTATGTGATGAATACCCGCAAGCTTCACACTGACGACACACCGGTAAAGGTattg                                                                             >  1:2462524/1‑147 (MQ=255)

                                                                        cccGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTATCGTTATGTAATGAATACCCGCCAGGTTCACACTGATGACACACCGGTAAAGGTACTgg                                                                            >  1:3474639/1‑150 (MQ=255)

                                                                        cccGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAg                                                                                   <  1:2173106/143‑1 (MQ=255)

                                                                         ccgccAGGG‑TGTCGACCTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTAATGGc                                                                           >  1:2561507/5‑150 (MQ=255)

                                                                         ccgccAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGc                                                                           >  1:294410/5‑150 (MQ=255)

                                                                            ccAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACGCCGGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCAcc                                                                        <  1:1591813/149‑1 (MQ=255)

                                                                             cAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCAGTGAATGATGCCCTGTACCGTTATGTAATGAACACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCg                                                                       >  1:2135220/1‑150 (MQ=255)

                                                                               ggg‑TGTCGAACTGAGCCGTGCATTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACGCCGCTGAATGATGCCCTGTACCGTTATGTGATGAATACCCGCAAGCTTCACACTGACGACACACCGGTAAAGGTACTGGCACCGGa                                                                     <  1:1956260/150‑1 (MQ=255)

                                                                                 g‑tgtCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAATTAATGACACCGGTGAATGATGCCTTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCgggtc                                                                   >  1:957141/1‑147 (MQ=255)

                                                                                   tgtCGAACTGAGCCGTGCATTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACGCCGCTGAATGATGCCCTGTACCGTTATGTGATGAATACCCGCAAGCTTCACACTGACGACACACCGGTAAAGGTACTGGCACCGGACCt                                                                  >  1:640978/1‑150 (MQ=255)

                                                                                   tgtCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTATACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCgggtca                                                                  <  1:1910510/150‑5 (MQ=255)

                                                                                    gtCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCgggtcag                                                                 >  1:673586/1‑145 (MQ=255)

                                                                                     tCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCgggtcaga                                                                >  1:1068899/1‑144 (MQ=255)

                                                                                       gAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCgggtcagaaa                                                              <  1:3322791/150‑9 (MQ=255)

                                                                                        aaCTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCgggtcagaaaa                                                             <  1:1998869/150‑10 (MQ=255)

                                                                                          cTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCgggtcagaaaaag                                                           <  1:517381/150‑12 (MQ=255)

                                                                                          cTGAGCCGAGCCTTACTCTCAAACTTGGTTGACGCGTGCTGCCAGTTAAGGACACCGGTGAATGATGCCCTGTACAGTGATGTAATGAAGACCTGCAAGGTTCACACTGATGAAACGCCGGTAAAGGTACTGGCACCgggtcagaaaaag                                                           <  1:3695411/150‑12 (MQ=255)

                                                                                           tGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCggg                                                                     >  1:4071210/1‑138 (MQ=255)

                                                                                            gAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTCAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCgggtcagaaaaaggc                                                         <  1:3958011/150‑14 (MQ=255)

                                                                                             aGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCgggtcagaaaaaggcg                                                        <  1:3362532/150‑15 (MQ=255)

                                                                                             aGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCgggtcagaaaaaggcg                                                        <  1:384937/150‑15 (MQ=255)

                                                                                                 gTGCATTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACGCCGCTGAATGATGCCCTGTACCGTTATGTGATGAATACCCGCAAGCTTCACACTGACGACACACCGGTAAAGGTACTGGCACCGGACCTGAAAAAGACGaaaa                                                    >  1:103863/1‑150 (MQ=255)

                                                                                                      ttACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACGCCGCTGAATGATGCCCTGTACCGTTATGTGATGAATACCCGCAAGCTTCACACTGACGACACACCGGTAAAGGTACTGGCACCGGACCTGAAAAAGACGAAAACAggg                                               <  1:2086596/150‑1 (MQ=255)

                                                                                                      ttACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAACACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAggg                                               >  1:1450339/1‑150 (MQ=255)

                                                                                                         ctctCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGgcgt                                            >  1:939060/1‑149 (MQ=255)

                                                                                                           ctcCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATTAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGgcgtat                                          >  1:3808862/1‑147 (MQ=255)

                                                                                                           ctcCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGgcgtat                                          >  1:3203077/1‑147 (MQ=255)

                                                                                                              cAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGTGAAAACAGGGCGTATCTg                                       >  1:2303053/1‑150 (MQ=255)

                                                                                                              cAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTg                                       >  1:1618056/1‑150 (MQ=255)

                                                                                                               aaCTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTgg                                      >  1:1871138/1‑150 (MQ=255)

                                                                                                               aaCTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTgg                                      <  1:259755/150‑1 (MQ=255)

                                                                                                                  tGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACg                                   <  1:3921187/150‑1 (MQ=255)

                                                                                                                  tGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACg                                   <  1:2061648/150‑1 (MQ=255)

                                                                                                                          cgcgTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCg                           >  1:1236091/1‑150 (MQ=255)

                                                                                                                           gcgTGCTGCCAGTTAATGACGCCGCTGAATGATGCCCTGTACCGTTATGTGATGAATACCCGCAAGCTTCACACTGACGACACACCGGTAAAGGTACTGGCACCGGACCTGAAAAAGACGAAAACAGGGCGCATCTGGACGTATGTCCgg                          >  1:1827275/1‑150 (MQ=255)

                                                                                                                              tgctgcCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGATGTATGTCCGGgat                       <  1:772988/150‑1 (MQ=255)

                                                                                                                               gctgcCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGgatg                      >  1:2397615/1‑150 (MQ=255)

                                                                                                                                ctgcCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGgatga                     >  1:1556483/1‑150 (MQ=255)

                                                                                                                                 tgcCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGgatgat                    >  1:115769/1‑150 (MQ=255)

                                                                                                                                  gcCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGGATGATc                   >  1:2971424/1‑150 (MQ=255)

                                                                                                                                       ttAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGGATGATCGCAAt              <  1:1196614/150‑1 (MQ=255)

                                                                                                                                        tAATGACGCCGCTGAATGATGCCCTGTACCGTTATGTGATGAATACCCGCAAGCTTCACACTGACGACACACCGGTAAAGGTACTGGCACCGGACCTGAAAAAGACGAAAACAGGGCGCATCTGGACGTATGTCCGGGATGATCGCAATg             <  1:2676953/150‑1 (MQ=255)

                                                                                                                                        tAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTGTCTGGACGTATGTCCGGGATGATCGCAATg             <  1:1005335/150‑1 (MQ=255)

                                                                                                                                        tAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGGATGATCGCAATg             >  1:1404208/1‑150 (MQ=255)

                                                                                                                                          aTGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGGATGATCGCAATgtg           >  1:2339369/1‑148 (MQ=255)

                                                                                                                                          aTGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCGGGTCAGAAAAAGGCGAAAACAGGGCGTATCTGGACGTATGTCCGGGATGATCGCAATgtg           >  1:69882/1‑148 (MQ=255)

                                                                                                                                                  gcTGAATGATGCCCTGTACCGTTATGTGATGAATACCCGCAAGCTTCACACTGACGACACACCGGTAAAGGTACTGGCACCGGACCTGAAAAAGACGAAAACAGGGCGCATCTGGACGTATGTCCGGGATGATCGCAATGCGGGTTCGTc   >  1:1512290/1‑150 (MQ=255)

GATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACACCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGACAGGG‑TGTCGAACTGAGCCGTGCATTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACGCCGCTGAATGATGCCCTGTACCGTTATGTGATGAATACCCGCAAGCTTCACACTGACGACACACCGGTAAAGGTACTGGCACCGGACCTGAAAAAGACGAAAACAGGGCGCATCTGGACGTATGTCCGGGATGATCGCAATGCGGGTTCGTCA  >  Xen14_143/554‑849

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`