BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	Xen14_143	651	T→G	intergenic (‑345/–)	Xen14_04759 ← / –	hypothetical protein/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Xen14_143	651	0	T	G	87.4%	3677.6 / inf	1425	intergenic (‑345/–)	Xen14_04759/–	hypothetical protein/–
Reads supporting (aligned to +/- strand): ref base T (77/103); new base G (566/678); total (643/782)
Fisher's exact test for biased strand distribution p-value = 5.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1428 total aligned reads displayed.

GTTGAAGCACCGGCGCCGTCCCGCCCGATAGAGCGTGGTATCGCGGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACACCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGACAGGG‑TGTCGAACTGAGCCGTGCATTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACGCCGCTGAATGATGCCCTGTACCGTTATGTGATGAATACCCGCAAGCTTCACACTGACGACACACCGGTAAAGGTACTGGCACCGGACCTGAAAAAGACGAAAAC  >  Xen14_143/502‑800

  tGAAGCACCGGCGCCGTCCCGCCCGATAGAGCGTGGTATCGCGGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACACCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGACAGGG‑TGTCGAACTGAGCCGTGc                                                                                                                                                     <  1:2958515/150‑1 (MQ=255)

             cgcCGTCCCGCCCGATAGAGCGTGGTATCGCGGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCa                                                                                                                                          <  1:1239759/150‑1 (MQ=37)

               ccGTCCCGCCCGATAGAGCGTGGTATCGCGGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCACAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAAc                                                                                                                                        <  1:3911706/150‑1 (MQ=25)

                 gTCCCGCCCGATAGAGCGTGGTATCGCGGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTTTCCAACTg                                                                                                                                      <  1:1419783/150‑1 (MQ=37)

                 gTCCCGCCCGATAGAGCGTGGTATCGCGGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTg                                                                                                                                      >  1:1912130/1‑150 (MQ=37)

                 gTCCCGCCCGATAGAGCGTGGTATCGCGGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGCCGAACTGAGCCGGGCCTTACTCTCCAACTg                                                                                                                                      >  1:1290287/1‑150 (MQ=37)

                   cccgcccgATAGAGCGTGGTATCGCGGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTggg                                                                                                                                    <  1:904984/150‑1 (MQ=37)

                    ccgcccgATAGAGCGTGGTATCGCGGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGt                                                                                                                                   <  1:1586878/150‑1 (MQ=37)

                    ccgcccgATAGAGCGTGGTATCGCGGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGt                                                                                                                                   >  1:2697958/1‑150 (MQ=37)

                     cgcccgATAGAGCGTGGTCTCGCGGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGCTAGGG‑TGTCGAACTGAGCCGGGCCTTCCTCTCCAACTGGGtt                                                                                                                                  >  1:960294/1‑150 (MQ=37)

                     cgcccgATAGAGCGTGGTATCGCGGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACACCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGACAGGG‑TGTCGAACTGAGCCGTGCATTACTCTCCAACTGGGtt                                                                                                                                  <  1:2987205/150‑1 (MQ=255)

                      gcccgATAGAGCGTGGTATCGCGGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTg                                                                                                                                 >  1:3373721/1‑150 (MQ=37)

                                gCGTGGTATCGCGGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCGGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGtgctg                                                                                                                       <  1:735501/150‑1 (MQ=38)

                                  gTGGTATCGCGGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGtg                                                                                                                          <  1:2666084/145‑1 (MQ=38)

                                   tGGTATCGCGGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACACCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGACAGGG‑TGTCGAACTGAGCCGTGCATTACTCTCCAACTGGGTTGACGCGTGCTGCCa                                                                                                                    <  1:3046504/150‑1 (MQ=255)

                                    ggTATCGCGGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAg                                                                                                                   <  1:590378/150‑1 (MQ=39)

                                    ggTATCGCGGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCAAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAg                                                                                                                   <  1:2926516/150‑1 (MQ=38)

                                         cgcgGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAAt                                                                                                              <  1:3441455/150‑1 (MQ=39)

                                         cgcgGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAAt                                                                                                              <  1:3818875/150‑1 (MQ=39)

                                          gcgGGCCCCGGATTCCTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATg                                                                                                             >  1:1185298/1‑150 (MQ=255)

                                          gcgGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATg                                                                                                             >  1:2828436/1‑150 (MQ=255)

                                          gcgGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATg                                                                                                             >  1:3080130/1‑150 (MQ=255)

                                          gcgGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACACCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGACAGGG‑TGTCGAACTGAGCCGTGCATTACTCTCCAACTGGGTTGACACGTGCTGCCAGTTAATg                                                                                                             <  1:176785/150‑1 (MQ=255)

                                            gggCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGAc                                                                                                           <  1:333638/150‑1 (MQ=255)

                                                cccGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGAcaccg                                                                                                       <  1:3020178/150‑1 (MQ=255)

                                                    gATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCAATGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGAcg                                                                                                                              <  1:1880550/123‑1 (MQ=255)

                                                      ttACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACACCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGACAGGG‑TGTCGAACTGAGCCGTGCATTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACGCCGCTGAat                                                                                                 <  1:4113037/150‑1 (MQ=255)

                                                           tGCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGc                                                                                            <  1:2233732/150‑1 (MQ=255)

                                                            gCCCGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGcc                                                                                           <  1:4158824/150‑1 (MQ=255)

                                                             cccGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAatgatg                                                                                             >  1:2455049/1‑147 (MQ=255)

                                                             cccGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGccc                                                                                          <  1:1999270/150‑1 (MQ=255)

                                                             cccGCGTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATAATGccc                                                                                          >  1:2747118/1‑150 (MQ=255)

                                                                 cgTGTTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTa                                                                                      <  1:1121317/150‑1 (MQ=255)

                                                                  gtgtTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTAc                                                                                     <  1:647361/150‑1 (MQ=255)

                                                                   tgtTAACGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTAcc                                                                                    <  1:3621548/150‑1 (MQ=255)

                                                                       aaCGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTa                                                                                <  1:1394131/150‑1 (MQ=255)

                                                                       aaCGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTa                                                                                <  1:2498166/150‑1 (MQ=255)

                                                                         cGGGAAAATACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTAtg                                                                              >  1:2864760/1‑150 (MQ=255)

                                                                             aaaaTACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTAtgtaat                                                                          <  1:4064400/150‑4 (MQ=255)

                                                                             aaaaTACTGCGAACACCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGACAGGG‑TGTCGAACTGAGCCGTGCATTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACGCCGCTGAATGATGCCCTGTACCGTTATGtgat                                                                          <  1:3402172/150‑1 (MQ=255)

                                                                             aaaaTACTGCGAACACCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGACAGGG‑TGTCGAACTGAGCCGTGCATTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACGCCGCTGAATGATGCCCTGTACCGTTATGtgat                                                                          <  1:940164/150‑1 (MQ=255)

                                                                              aaaTACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGtaatg                                                                         <  1:426992/150‑1 (MQ=255)

                                                                                acaCTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCG‑CAGGGGTGTCGAACTGAGCCGGGCCTTACTCTCCCACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGaa                                                                       >  1:2635533/3‑150 (MQ=255)

                                                                                aTACTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGaa                                                                       <  1:1742070/150‑1 (MQ=255)

                                                                                   cTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGaacac                                                                    >  1:2778081/1‑147 (MQ=255)

                                                                                   cTGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAACGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGaacac                                                                    >  1:2200587/1‑147 (MQ=255)

                                                                                    tGCGAACATCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATAcc                                                                   <  1:496114/150‑1 (MQ=255)

                                                                                      cGAACATCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAACACCCg                                                                 >  1:459286/1‑150 (MQ=255)

                                                                                      cGAACATCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAACACCCg                                                                 >  1:3985413/1‑150 (MQ=255)

                                                                                           atcTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAgg                                                            <  1:3146652/148‑2 (MQ=255)

                                                                                             cTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAggtt                                                          <  1:1062305/150‑4 (MQ=255)

                                                                                              tGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTc                                                         >  1:1522748/1‑150 (MQ=255)

                                                                                              tGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTc                                                         >  1:3556991/1‑150 (MQ=255)

                                                                                                ccACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTGCCGTTATGTAATGAATACCCGCAAGGTTcac                                                       <  1:1357103/150‑1 (MQ=255)

                                                                                                ccACTGTATCGTCAGAGTGAAATCTTTGCCCGACAGGG‑TGTCGAACTGAGCCGTGCATTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACGCCGCTGAATGATGCCCTGTACCGTTATGTGATGAATACCCGCAAGCTTcac                                                       <  1:2254220/150‑1 (MQ=255)

                                                                                                 cACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTcaca                                                      >  1:800955/1‑150 (MQ=255)

                                                                                                 cACTGTATCGTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAACACCCGCAAGGTTcaca                                                      >  1:3185345/1‑150 (MQ=255)

                                                                                                       aTCGTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTgatg                                                <  1:2388877/150‑3 (MQ=255)

                                                                                                       aTCGTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTgatg                                                <  1:3859481/150‑3 (MQ=255)

                                                                                                       aTCGTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTgatg                                                <  1:2104092/150‑3 (MQ=255)

                                                                                                       aTCGTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTgatg                                                <  1:3686948/150‑3 (MQ=255)

                                                                                                       aTCGTCAGAGTGAAATCTTTGCCCGACAGGG‑TGTCGAACTGAGCCGTGCATTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACGCCGCTGAATGATGCCCTGTACCGTTATGTGATGAATACCCGCAAGCTTCACACTgacg                                                >  1:2036588/1‑150 (MQ=255)

                                                                                                         cGTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAACACCCGCAAGGTTCACACTgatgac                                              >  1:4014720/1‑150 (MQ=255)

                                                                                                          gTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGaca                                             <  1:769565/150‑1 (MQ=255)

                                                                                                          gTCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAACACCCGCAAGGTTCACACTGATGaca                                             >  1:3528106/1‑150 (MQ=255)

                                                                                                           tCAGAGTGAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGacac                                            >  1:3266185/1‑150 (MQ=255)

                                                                                                                  gAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTa                                     >  1:1653977/1‑150 (MQ=255)

                                                                                                                  gAAATCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTa                                     >  1:2357668/1‑150 (MQ=255)

                                                                                                                    aaTCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTaaa                                   <  1:3305901/150‑1 (MQ=255)

                                                                                                                    aaTCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTaaa                                   <  1:170752/150‑1 (MQ=255)

                                                                                                                    aaTCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTaaa                                   >  1:131527/1‑150 (MQ=255)

                                                                                                                    aaTCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAACACCCGCAAGGTTCACACTGATGACACAACGGTaaa                                   >  1:849183/1‑150 (MQ=255)

                                                                                                                     aTCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAACACCCGCAAGGTTCACACTGATGACACACCGGTAAAg                                  >  1:2312938/1‑150 (MQ=255)

                                                                                                                      tCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAgg                                 <  1:1208818/150‑1 (MQ=255)

                                                                                                                      tCTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTAATGACACACCGGTAAAgg                                 <  1:3489500/150‑1 (MQ=255)

                                                                                                                      tCTTTGCCCGACAGGG‑TGTCGAACTGAGCCGTGCATTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACGCCGCTGAATGATGCCCTGTACCGTTATGTGATGAATACCCGCAAGCTTCACACTGACGACACACCGGTAAAgg                                 <  1:543666/150‑1 (MQ=255)

                                                                                                                       cTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGt                                >  1:4181220/1‑150 (MQ=255)

                                                                                                                       cTTTGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGATGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGt                                >  1:1005738/1‑150 (MQ=255)

                                                                                                                        tttGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTATTCTCCAACTGGNTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAACACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTa                               >  1:3949334/1‑150 (MQ=255)

                                                                                                                        tttGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTa                               >  1:3582946/1‑150 (MQ=255)

                                                                                                                        tttGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTa                               >  1:1469642/1‑150 (MQ=255)

                                                                                                                         ttGCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTAc                              <  1:692651/150‑1 (MQ=255)

                                                                                                                           gCCCGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTaacccgcata                            >  1:440663/1‑142 (MQ=255)

                                                                                                                            cccGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGGCGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTATTgg                           <  1:2071934/150‑1 (MQ=255)

                                                                                                                            cccGCCAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAg                                  <  1:2173106/143‑1 (MQ=255)

                                                                                                                             ccgccAGGG‑TGTCGACCTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTAATGGc                          >  1:2561507/5‑150 (MQ=255)

                                                                                                                             ccgccAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGc                          <  1:1491575/146‑1 (MQ=255)

                                                                                                                                ccAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACTCCGGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCAcc                       >  1:1613642/2‑150 (MQ=255)

                                                                                                                                 cAGGG‑TGTCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCAGTGAATGATGCCCTGTACCGTTATGTAATGAACACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCg                      >  1:2135220/1‑150 (MQ=255)

                                                                                                                                   ggg‑TGTCGAACTGAGCCGTGCATTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACGCCGCTGAATGATGCCCTGTACCGTTATGTGATGAATACCCGCAAGCTTCACACTGACGACACACCGGTAAAGGTACTGGCACCGGa                    <  1:1956260/150‑1 (MQ=255)

                                                                                                                                     g‑tgtCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATNATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCgggtc                  <  1:3752610/150‑4 (MQ=255)

                                                                                                                                        gtCGAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAACACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCgggtcag                >  1:3787092/1‑145 (MQ=255)

                                                                                                                                           gAACTGAGCCGGGCCTTACTCTCCAACTGGGTTGGCGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCgggtcagaaa             <  1:1096005/150‑9 (MQ=255)

                                                                                                                                            aaCTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCgggtcagaaaa            >  1:2593204/1‑141 (MQ=255)

                                                                                                                                            aaCTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCgggtcagaaaa            <  1:820357/150‑10 (MQ=255)

                                                                                                                                              cTGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCgggtcagaaaaag          >  1:4215505/1‑139 (MQ=255)

                                                                                                                                               tGAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCgggtcagaaaaagg         <  1:3226318/150‑13 (MQ=255)

                                                                                                                                                gAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCgggtcagaaaaaggc        <  1:115054/150‑14 (MQ=255)

                                                                                                                                                gAGCCGGGCCTTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACACCGGTGAATGATGCCCTGTACCGTTATGTAATGAATACCCGCAAGGTTCACACTGATGACACACCGGTAAAGGTACTGGCACCgggtcagaaaaaggc        <  1:1842471/150‑14 (MQ=255)

                                                                                                                                                  gCCGTGCATTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACGCCGCTGAATGATGCCCTGTACCGTTATGTGATGAATACCCGCAAGCTTCACACTGACGACACACCGGTAAAGGTACTGGCACCGGACCTGAAAAAGACGa      >  1:2419926/1‑150 (MQ=255)

GTTGAAGCACCGGCGCCGTCCCGCCCGATAGAGCGTGGTATCGCGGGCCCCGGATTACTTGCCCGCGTGTTAACGGGAAAATACTGCGAACACCTGCCACTGTATCGTCAGAGTGAAATCTTTGCCCGACAGGG‑TGTCGAACTGAGCCGTGCATTACTCTCCAACTGGGTTGACGCGTGCTGCCAGTTAATGACGCCGCTGAATGATGCCCTGTACCGTTATGTGATGAATACCCGCAAGCTTCACACTGACGACACACCGGTAAAGGTACTGGCACCGGACCTGAAAAAGACGAAAAC  >  Xen14_143/502‑800

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`