Predicted mutation
evidence seq id position mutation annotation gene description
RA Xen14_143 155 G→A A51V (GCG→GTG)  Xen14_04759 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Xen14_1431550GA80.3% 1387.3 / 277.4 790A51V (GCG→GTG) Xen14_04759hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (78/77);  new base A (324/308);  total (404/386)
Fisher's exact test for biased strand distribution p-value = 8.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 792 total aligned reads displayed.

GAACTGGAAAGCCAGCTCCGTCAGAAAGACCAGCAACTGAGTCTGGTTGAAGAGACGGAGGCCTTC‑‑‑‑‑‑‑CTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGGCTCAGATAGAAAAACTGCGCCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACAGGCTGAGGCCCTGCTGAAACAACGCGAACAGGACAGCGATCGTTACAGTGGGCGGGAAGACGATCCACAGGTTCCCCGCCAGTTGCGACAGTCGCGC  >  Xen14_143/8‑303
                                                                                                                                                          |                                                                                                                                                    
gAACTGGAAAGCCAGCTCCGTCAGAAAGACCAGCAACTGAGTCTGGTTGAAGAGACGGAGGCCTTC‑‑‑‑‑‑‑CTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGGCTCAGATAGAAAAACTGCGcc                                                                                                                                                    >  1:2421056/1‑150 (MQ=255)
          gccagctccgtcagaaagaccagcaactgagtctggttgaagngacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGa                                                                                                                                                 <  1:2705017‑M1/87‑1 (MQ=255)
          gccagctccgtcagaaagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGa                                                                                                                                                 <  1:3154689‑M1/87‑1 (MQ=255)
          gccagctccgtcagaaagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGa                                                                                                                                                 <  1:2727720‑M1/87‑1 (MQ=255)
            cagctccgtcagaaagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATg                                                                                                                                               <  1:4072124‑M1/89‑1 (MQ=255)
            cagctccgtcagaaagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATg                                                                                                                                               <  1:2227111‑M1/89‑1 (MQ=255)
            cagctccgtcagaaagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATg                                                                                                                                               <  1:1185287‑M1/89‑1 (MQ=255)
              gctccgtcagaaagaccagcaactgagtctggttgaagagactgaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACCGCACCGGATGCt                                                                                                                                             >  1:2831763‑M1/60‑150 (MQ=255)
               ctccgtcagaaagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTg                                                                                                                                            <  1:48015‑M1/92‑1 (MQ=255)
                tccgtcagaaagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGt                                                                                                                                           <  1:3404070‑M1/93‑1 (MQ=255)
                tccgtcagaaagaccagcaactgagtctggttgaagagaaggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGt                                                                                                                                           >  1:183690‑M1/58‑150 (MQ=255)
                 ccGTCAGAAAGACCAGCAACTGAGTCTGGTTGAAGAGACGGAGGCCTTC‑‑‑‑‑‑‑CTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGGCTCAGATAGAAAAACTGCGCCGGATACTGTTCGGTAcc                                                                                                                                   <  1:142306/150‑1 (MQ=255)
                  cgtcagaaagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTc                                                                                                                                         <  1:2902257‑M1/95‑1 (MQ=255)
                  cgtcagaaagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACCGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTc                                                                                                                                         >  1:3685539‑M1/56‑150 (MQ=255)
                   gtcagaaagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCg                                                                                                                                        >  1:1075256‑M1/55‑150 (MQ=255)
                   gtcagaaagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAAAGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCg                                                                                                                                        <  1:3451628‑M1/96‑1 (MQ=255)
                    tcagaaagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCgg                                                                                                                                       >  1:1648045‑M1/54‑150 (MQ=255)
                     cagaaagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACCGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGt                                                                                                                                      >  1:2952102‑M1/53‑150 (MQ=255)
                      agaaagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTa                                                                                                                                     >  1:2784750‑M1/52‑150 (MQ=255)
                       gAAAGACCAGCAACTGAGTCTGGTTGAAGAGACGGAGGCCTTC‑‑‑‑‑‑‑CTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGGCTCAGATAGAAAAACTGCGCCGGATGCTGTTCGGTACCCGTTCt                                                                                                                             <  1:756502/150‑1 (MQ=255)
                        agagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTAcc                                                                                                                                   >  1:382454‑M1/50‑150 (MQ=255)
                        aaagaccagcaactgagtctggttgaggagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCg                                                                                                                                        <  1:4425578‑M1/96‑1 (MQ=255)
                        aaagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGGCTCAGATAGAAAAACTGCGCCGGATGCTGt                                                                                                                                           <  1:3008836‑M1/93‑1 (MQ=255)
                         aagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTAccc                                                                                                                                  <  1:4148224‑M1/102‑1 (MQ=255)
                         aagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTAccc                                                                                                                                  <  1:1993700‑M1/102‑1 (MQ=255)
                          agaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCg                                                                                                                                 >  1:3096398‑M1/48‑150 (MQ=255)
                          agaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCg                                                                                                                                 >  1:2654904‑M1/48‑150 (MQ=255)
                          agaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCg                                                                                                                                 >  1:2506873‑M1/48‑150 (MQ=255)
                          agaccagcaaatgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATGGAAAAACTGCACAGGATGCTGTTCGGTACCCg                                                                                                                                 <  1:2682811‑M1/103‑1 (MQ=255)
                           gACCAGCAACTGAGTCTGGTTGAAGAGACGGAGGCCTTC‑‑‑‑‑‑‑CTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGGCTCAGATAGAAAAACTGCGCCGGATGCTGTTCGGTACCCGTTCTGaaa                                                                                                                         >  1:3323970/1‑150 (MQ=255)
                            accagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTATTCGGTACCCGtt                                                                                                                               <  1:1294361‑M1/105‑1 (MQ=255)
                             ccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTc                                                                                                                              <  1:3929456‑M1/106‑1 (MQ=255)
                             ccAGCAACTGAGTCTGGTTGAAGAGACGGAGGCCTTC‑‑‑‑‑‑‑CTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACCGGAAATAGAACATCTGCGGGCTCAGATAGAAAAACTGCGCCGGATGCTGTTCGGTACCCGTTCTGaaaaa                                                                                                                       <  1:620472/150‑1 (MQ=255)
                             ccAGCAACTGAGTCTGGTTGAAGAGAAGGAGGCCTTCCTGCGCTCTGCACTGGCACTTGCCGAAGAAAAGATAGAAGAAGATGAAAGGGAAATAGAACATCTGCGGACTCAGATCGAAAAACTGCACCGGATGCTGTTCGGTACCCGtcc                                                                                                                              >  1:696302/1‑148 (MQ=255)
                              cagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCt                                                                                                                             <  1:1569771‑M1/107‑1 (MQ=255)
                               agcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTg                                                                                                                            >  1:777820‑M1/43‑150 (MQ=255)
                                gcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGa                                                                                                                           <  1:378099‑M1/109‑1 (MQ=255)
                                  aactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGaaa                                                                                                                         >  1:1265729‑M1/40‑150 (MQ=255)
                                   actgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGaaaa                                                                                                                        >  1:4113655‑M1/39‑150 (MQ=255)
                                   actgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGaaaa                                                                                                                        >  1:3970479‑M1/39‑150 (MQ=255)
                                     tgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAAc                                                                                                                      >  1:1611069‑M1/37‑150 (MQ=255)
                                         tctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGcg                                                                                                                  >  1:1519630‑M1/33‑150 (MQ=255)
                                         tctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGcg                                                                                                                  <  1:3070599‑M1/118‑1 (MQ=255)
                                            ggTTGAAGAGACGGAGGCCTTC‑‑‑‑‑‑‑CTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAGCATCTGCGGGCTCAGATAGAAAAACTGCGCCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGtt                                                                                                        >  1:2762850/1‑150 (MQ=255)
                                             gttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAATGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGcgtcgt                                                                                                              >  1:2978790‑M1/29‑150 (MQ=255)
                                             gttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGcgtcgt                                                                                                              <  1:580481‑M1/122‑1 (MQ=255)
                                             gttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGcgtcgt                                                                                                              <  1:2629492‑M1/122‑1 (MQ=255)
                                                gaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGaa                                                                                                           <  1:3346128‑M1/125‑1 (MQ=255)
                                                gaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGaa                                                                                                           >  1:2142175‑M1/26‑150 (MQ=255)
                                                 aagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAg                                                                                                          >  1:2101260‑M1/25‑150 (MQ=255)
                                                 aagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAg                                                                                                          <  1:446690‑M1/126‑1 (MQ=255)
                                                 aagagacggaggccttcctgcgctCTACACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAACACTGCGTCGTGAAg                                                                                                          <  1:3218248‑M1/126‑1 (MQ=255)
                                                 aagagacggaggccttcctgcgctCTACACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAg                                                                                                          <  1:2336957‑M1/126‑1 (MQ=255)
                                                 aaGAGACGGAGGCCTTC‑‑‑‑‑‑‑CTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGGCTCAGATAGAAAAACTGCGCCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACa                                                                                                   >  1:3893600/1‑150 (MQ=255)
                                                  agagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGt                                                                                                         >  1:2843859‑M1/24‑150 (MQ=255)
                                                  agagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGt                                                                                                         <  1:531371‑M1/127‑1 (MQ=255)
                                                     gacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGa                                                                                                      <  1:3710761‑M1/130‑1 (MQ=255)
                                                     gacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGa                                                                                                      <  1:3614675‑M1/130‑1 (MQ=255)
                                                     gacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACCGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGa                                                                                                      <  1:3491759‑M1/130‑1 (MQ=255)
                                                      acggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGaa                                                                                                     >  1:4016662‑M1/20‑150 (MQ=255)
                                                      aCGGAGGCCTTC‑‑‑‑‑‑‑CTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGGCTCAGATAGAAAAACTGCGCCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACAGGCTg                                                                                              >  1:1484773/1‑150 (MQ=255)
                                                       cggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGCAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAAc                                                                                                    <  1:925136‑M1/132‑1 (MQ=255)
                                                       cggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGCACATCTGCGAACTCAGACAGAAAAAATACACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGACGTTgaaa                                                                                                    <  1:2877720‑M1/132‑2 (MQ=255)
                                                       cggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAAc                                                                                                    >  1:2536508‑M1/19‑150 (MQ=255)
                                                         gaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGAACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGaactg                                                                                                  >  1:1855764‑M1/17‑148 (MQ=255)
                                                           ggCCTTC‑‑‑‑‑‑‑CTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGGCTCAGATAGAAAAACTGCGCCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACAGGCTGAGGcc                                                                                         >  1:1924948/1‑150 (MQ=255)
                                                             ccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACTGGCTg                                                                                              <  1:2298473‑M1/138‑1 (MQ=255)
                                                              cttcctgcgctCTGCACTGGCACGTGCCGAAGAAAGGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACTGGCTGa                                                                                             <  1:102282‑M1/139‑1 (MQ=255)
                                                               ttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACTGGCTGAg                                                                                            >  1:1894825‑M1/11‑150 (MQ=255)
                                                                 cctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCGGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACTGGCTGAGGc                                                                                          >  1:386365‑M1/9‑150 (MQ=255)
                                                                 cctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACTGGCTGAGGc                                                                                          >  1:2448348‑M1/9‑150 (MQ=255)
                                                                  ctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACTGGCTGAGgct                                                                                         <  1:2177402‑M1/143‑2 (MQ=255)
                                                                     cgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACTGGCTgaggctctg                                                                                      <  1:2273449‑M1/146‑5 (MQ=255)
                                                                      gctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACTGGCTGAGGCTctgc                                                                                     >  1:886358‑M1/4‑150 (MQ=255)
                                                                      gctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACTGGCTGAGGCTctgc                                                                                     <  1:1224010‑M1/147‑1 (MQ=255)
                                                                      gctCTACACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACTGGCTGAGGCTctgc                                                                                     <  1:3430828‑M1/147‑1 (MQ=255)
                                                                       ctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACTGGCTGAGGCTctgct                                                                                    <  1:3257822‑M1/148‑1 (MQ=255)
                                                                        tCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACTGGCTGAGGCTctgctg                                                                                   >  1:819681‑M1/2‑150 (MQ=255)
                                                                        tCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACTGGCTGAGGCTctgctg                                                                                   >  1:1399595‑M1/2‑150 (MQ=255)
                                                                           gCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAAc                                                                                                    <  1:738549/130‑1 (MQ=255)
                                                                           gCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACTGGCTGAGGCTCTGCTGaaa                                                                                <  1:3543342/150‑1 (MQ=25)
                                                                           gCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACTGGCTGAGGCTCTGCTGaaa                                                                                >  1:1112005/1‑150 (MQ=25)
                                                                           gCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACTGGCTGAGGCTCTGCTGaaa                                                                                >  1:1950663/1‑150 (MQ=25)
                                                                           gCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACTGGCTGAGGCTCTGCTGaaa                                                                                <  1:2209492/150‑1 (MQ=25)
                                                                           gCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACTGGCTGAGGCTCTGCTGaaa                                                                                <  1:208446/150‑1 (MQ=25)
                                                                           gCACGGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACTGGCTGAGGCTCTGCTGaaa                                                                                <  1:1329487/150‑1 (MQ=25)
                                                                            cACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACTGGCTGAGGCTCTGCTGAaac                                                                               <  1:423573/150‑1 (MQ=25)
                                                                              cTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACTGGCTGAGGCTCTGCTGAaacaa                                                                             >  1:967756/1‑150 (MQ=25)
                                                                              cTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACTGGCTGAGGCTCTGCTGAaacaa                                                                             >  1:3657822/1‑150 (MQ=25)
                                                                               tGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACTGGCTGAGGCTCTGCTGAaacaac                                                                            <  1:1723620/150‑1 (MQ=25)
                                                                                ggCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACTGGCCGAGGCTCTGCTGAAACAAcg                                                                           >  1:1752023/1‑150 (MQ=25)
                                                                                ggCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTACGTCGTGAAGTTGAACTGGCTGAGGCTCTGCTGAAACAAcg                                                                           >  1:1129614/1‑150 (MQ=25)
                                                                                            cgaagaGATCGAAGAAGATGAACGGGAAATACAATACTTGCGGGCTTATATAGAAGAACTGCACCGGCTGCTGTTTGGTACCTTTTCTGAAAAACTGCGTCGTGAAGTTGAGCTGGTTGAGGCTCTGCGGAAACAACGTGAACAGGACAg                                                               <  1:1001551/145‑1 (MQ=9)
                                                                                                         gaagaTGAACGGGAAATAGAACATCTGCGGGCTCAGATAGAAAAACTGCGCCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACAGGCTGAGGCCCTGCTGAAACAAcg                                                                           <  1:2018381/125‑1 (MQ=255)
                                                                                                               gAACGGGAAATAGAACATCTGCGGGCTCAGATAGAAAAACTGCGCCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACAGGCTGAGGCCCTGCTGAAACAACGCGAACAGGACAGCGATCGTTACAGTGGGCgg                                            <  1:1778554/150‑1 (MQ=255)
                                                                                                                  cGGGAAATAGAACATCTGCGGGCTCAGATAGAAAAACTGCGCCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACAGGCTGAGGCCCTGCTGAAACAACGCGAACAGGACAGCGATCGTTACAGTGGGCGGGaa                                         <  1:3735675/150‑1 (MQ=255)
                                                                                                                       aaTAGAACATCTGCGGGCTCAGATAGAAAAACTGCGCCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACAGGCTGAGGCCCTGCTGAAACAACGCGAACAGGACAGCGATCGTTACAGTGGGCGGGAAGACGa                                    >  1:652326/1‑150 (MQ=255)
                                                                                                                            aaCATCTGCGGGCTCAGATAGAAAAACTGCGCCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACAGGCTGAGGCCCTGCTGAAACAACGCGAACAGGGCAGCGATCGTTACAGTGGGCGGGAAGACGATCcac                               <  1:526681/150‑1 (MQ=255)
                                                                                                                                   gCGGGCTCAGATAGAAAAACTGCGCCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACAGGCTGAGGCCCTGCTGAAACAACGCGAACAGGACAGCGATCGTTACAGTGGGCGGGAAGACGATCCACAGGTTcc                        <  1:4176577/150‑1 (MQ=255)
                                                                                                                                                 aaaaaCTGCGCCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACAGGCTGAGGCCCTGCTGAAACAATGCGAACAGGACAGCGATCGTTACAGTGGGCGGGAAGACGATCCACAGGTTCCCCGCCAGTTGCGAc          >  1:3176954/1‑150 (MQ=255)
                                                                                                                                                          |                                                                                                                                                    
GAACTGGAAAGCCAGCTCCGTCAGAAAGACCAGCAACTGAGTCTGGTTGAAGAGACGGAGGCCTTC‑‑‑‑‑‑‑CTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGGCTCAGATAGAAAAACTGCGCCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACAGGCTGAGGCCCTGCTGAAACAACGCGAACAGGACAGCGATCGTTACAGTGGGCGGGAAGACGATCCACAGGTTCCCCGCCAGTTGCGACAGTCGCGC  >  Xen14_143/8‑303

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑