Predicted mutation
evidence seq id position mutation annotation gene description
RA Xen14_143 136 G→A S57S (AGC→AGT)  Xen14_04759 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Xen14_1431360GA83.6% 1836.5 / 265.8 938S57S (AGC→AGT) Xen14_04759hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (69/85);  new base A (400/384);  total (469/469)
Fisher's exact test for biased strand distribution p-value = 1.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 943 total aligned reads displayed.

‑‑‑‑‑‑‑‑‑‑‑‑‑‑CATTGCAGAACTGGAAAGCCAGCTCCGTCAGAAAGACCAGCAACTGAGTCTGGTTGAAGAGACGGAGGCCTTCCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGGCTCAGATAGAAAAACTGCGCCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACAGGCTGAGGCCCTGCTGAAACAACGCGAACAGGACAGCGATCGTTACAGTGGGCGGGAAGACGATCCACAGGTTCCCCGC  >  Xen14_143/1‑285
                                                                                                                                                     |                                                                                                                                                     
aaatacctcattcgcATTGCAGAACTGGAAAGCCAGCTCCGTCAGAAAGACCAGCAACTGAGTCTGGTTGAAGAGACGGAGGCCTTCCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCggg                                                                                                                                                       >  1:2164195/15‑150 (MQ=255)
      gcattgcagaactggaaagccagctccgtcagaaagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGa                                                                                                                                                 <  1:1684720‑M1/69‑1 (MQ=255)
       cattgcagaactggaaagccagctccgtcagaaagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACTAGAAATAGAACATCTGCGGACTCAGAt                                                                                                                                                >  1:3049932‑M1/81‑150 (MQ=255)
        attgcagaactggaaagccagctccgtcagaaagaccagcaactgagtctggttgaagagacggatgctttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATa                                                                                                                                               >  1:1125065‑M1/80‑150 (MQ=255)
        attgcagaactggaaagccagctccgtcagaaagaccagcaaatgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAAAGGGAAATAGAACATATGCGGACTCAGATa                                                                                                                                               <  1:2071359‑M1/71‑1 (MQ=255)
           gcagaactggaaagccagctccgtcagaaagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGaa                                                                                                                                            <  1:1295830‑M1/74‑1 (MQ=255)
            cagaactggaaagccagctccgtcagaaagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGaaa                                                                                                                                           <  1:3593537‑M1/75‑1 (MQ=255)
             agaactggaaagccagctccgtcagaaagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTTAGATAGaaaa                                                                                                                                          <  1:4060349‑M1/76‑1 (MQ=255)
              gaactggaaagccagctccgtcagaaagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAg                                                                                                                                              <  1:3404570‑M1/72‑1 (MQ=255)
               aactggaaagccagctccgtcagaaagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAAc                                                                                                                                        >  1:1069022‑M1/73‑150 (MQ=255)
               aactggaaagccagctccgtcagaaagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACGCAGATAGAAAAAc                                                                                                                                        >  1:3699534‑M1/73‑150 (MQ=255)
               aTTGCAGAACTGGAAAGCCAGCTCCGTCAGAAAGACCAGCAACTGAGTCTGGTTGAAGAGACGGAGGCCTTCCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGGCTCAGATAGAAAAAc                                                                                                                                        <  1:2353971/150‑1 (MQ=255)
                actggaaagccagctccgtcagaaagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACt                                                                                                                                       >  1:243347‑M1/72‑150 (MQ=255)
                  gCAGAACTGGAAAGCCAGCTCCGTCAGAAAGACCAGCAACTGAGTCTGGTTGAAGAGACGGAGGCCTTCCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGGCTCAGATAGAAAAACTgc                                                                                                                                     <  1:160006/150‑1 (MQ=255)
                    gaaagccagctccgtcagaaagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAActgcac                                                                                                                                   <  1:3226346‑M1/83‑3 (MQ=255)
                      aagccagctccgtcagaaagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAaactgcaccg                                                                                                                                 <  1:1573809‑M1/85‑5 (MQ=255)
                        gccagctccgtcagaaagaccagtaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGa                                                                                                                               <  1:4211404‑M1/87‑1 (MQ=255)
                        gccagctccgtcagaaagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGa                                                                                                                               >  1:757288‑M1/64‑150 (MQ=255)
                        gccagctccgtcagaaagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGa                                                                                                                               <  1:3673233‑M1/87‑1 (MQ=255)
                        cTGGAAAGCCAGCTCCGTCAGAAAGACCAGCAACTGAGTCTGGTTGAAGAGACGGAGGCCTTCCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGGCTCAGATAGaaaa                                                                                                                                          <  1:2243465/139‑1 (MQ=255)
                           gAAAGACAGCTCCGTCAGAAAGACCAGCAACTGAGTCGGGTTGAAGAGACGGAGGCCTTCCTGAACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGGCTCAGATAGAAAAACTGCGCCGGATGc                                                                                                                            <  1:467826/150‑1 (MQ=255)
                                cgtcagaaagaccagcaactgagtctggttgacgagccggaggcattccggcgctCTGCACTGACACGTGCAGAAGAACAGATCGAAGAAGATGAACGGTCAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTc                                                                                                                       <  1:4379813‑M1/95‑1 (MQ=255)
                                cgtcagaaagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTc                                                                                                                       <  1:3471682‑M1/95‑1 (MQ=255)
                                cgtcagaaagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACAGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTc                                                                                                                       >  1:1266330‑M1/56‑150 (MQ=255)
                                ccAGCTCCGTCAGAAAGACCAGCAACTGAGTCTGGTTGAAGAGACGGAGGCCTTCCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGGCTCAGATAGAAAAACTGCGCCGGATGCTGTTc                                                                                                                       >  1:2689022/1‑150 (MQ=255)
                                 gtcagaaagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCg                                                                                                                      <  1:740567‑M1/96‑1 (MQ=255)
                                 gtcagaaagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCg                                                                                                                      <  1:1182825‑M1/96‑1 (MQ=255)
                                   cagaaagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGt                                                                                                                    <  1:3149947‑M1/98‑1 (MQ=255)
                                   cagaaagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGt                                                                                                                    >  1:3906261‑M1/53‑150 (MQ=255)
                                    agaaagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAAc                                                                                                                                        <  1:375024‑M1/78‑1 (MQ=255)
                                    agaaagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTa                                                                                                                   >  1:2784750‑M1/52‑150 (MQ=255)
                                      ccGTCAGAAAGACCAGCAACTGAGTCTGGTTGAAGAGACGGAGGCCTTCCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGGCTCAGATAGAAAAACTGCGCCGGATACTGTTCGGTAcc                                                                                                                 <  1:142306/150‑1 (MQ=255)
                                       aagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTAccc                                                                                                                <  1:1993700‑M1/102‑1 (MQ=255)
                                       aagaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCAGTAccc                                                                                                                <  1:1099715‑M1/102‑1 (MQ=255)
                                        agaccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCg                                                                                                               >  1:2759877‑M1/48‑150 (MQ=255)
                                          accagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGtt                                                                                                             <  1:3085224‑M1/105‑1 (MQ=255)
                                           ccagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACCGCACCGGATGCTGTTCGGTACCCGTTc                                                                                                            <  1:3541824‑M1/106‑1 (MQ=255)
                                            gAAAGACCAGCAACTGAGTCTGGTTGAAGAGACGGAGGCCTTCCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGGCTCAGATAGAAAAACTGCGCCGGATGCTGTTCGGTACCCGTTCt                                                                                                           <  1:2723984/150‑1 (MQ=255)
                                            cagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCt                                                                                                           >  1:4002532‑M1/44‑150 (MQ=255)
                                            cagcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCt                                                                                                           <  1:3274430‑M1/107‑1 (MQ=255)
                                             agcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTg                                                                                                          >  1:777823‑M1/43‑150 (MQ=255)
                                             agcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTg                                                                                                          >  1:388016‑M1/43‑150 (MQ=255)
                                              gcaactgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGa                                                                                                         <  1:396836‑M1/109‑1 (MQ=255)
                                                 actgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGaaaa                                                                                                      >  1:2920673‑M1/39‑150 (MQ=255)
                                                   tgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAAc                                                                                                    >  1:721149‑M1/37‑150 (MQ=255)
                                                   tgagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAAc                                                                                                    >  1:513850‑M1/37‑150 (MQ=255)
                                                    gagtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACt                                                                                                   >  1:4107926‑M1/36‑150 (MQ=255)
                                                     agtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTg                                                                                                  >  1:3329905‑M1/35‑150 (MQ=255)
                                                      gtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGc                                                                                                 <  1:2997720‑M1/117‑1 (MQ=255)
                                                      gtctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGc                                                                                                 >  1:285336‑M1/34‑150 (MQ=255)
                                                       tctggttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAAAGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGcg                                                                                                <  1:3789164‑M1/118‑1 (MQ=255)
                                                       agaacatgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGcg                                                                                                >  1:1621412‑M1/33‑150 (MQ=255)
                                                         tggttgaagagacggaggccttcctgcgctCTGCACTGGCACGCGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGcgtc                                                                                              >  1:925905‑M1/31‑150 (MQ=255)
                                                           gttgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGcgtcgt                                                                                            <  1:580481‑M1/122‑1 (MQ=255)
                                                             tgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGa                                                                                          >  1:640204‑M1/27‑150 (MQ=255)
                                                             tgaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGa                                                                                          >  1:3844896‑M1/27‑150 (MQ=255)
                                                              gaagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGaa                                                                                         >  1:2142175‑M1/26‑150 (MQ=255)
                                                               ttttgataaaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAATGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAg                                                                                        >  1:2284713‑M1/25‑150 (MQ=255)
                                                               aagagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAg                                                                                        >  1:2101260‑M1/25‑150 (MQ=255)
                                                                agagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGt                                                                                       <  1:3179330‑M1/127‑1 (MQ=255)
                                                                 gagacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGtt                                                                                      >  1:2675428‑M1/23‑150 (MQ=255)
                                                                  agacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTg                                                                                     <  1:1912272‑M1/129‑1 (MQ=255)
                                                                   gacgtaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGa                                                                                    >  1:2646261‑M1/21‑150 (MQ=255)
                                                                   gacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGa                                                                                    <  1:669805‑M1/130‑1 (MQ=255)
                                                                   gacggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACCGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGa                                                                                    <  1:3491759‑M1/130‑1 (MQ=255)
                                                                    acggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGaa                                                                                   <  1:1522225‑M1/131‑1 (MQ=255)
                                                                     cggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGGAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAAc                                                                                  >  1:2536504‑M1/19‑150 (MQ=255)
                                                                     cggaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAAc                                                                                  >  1:443879‑M1/19‑150 (MQ=255)
                                                                       gaggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGaactg                                                                                >  1:2888055‑M1/17‑148 (MQ=255)
                                                                         ggccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTtgaactggc                                                                              <  1:2018866‑M1/136‑5 (MQ=255)
                                                                           ccttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACTGGCTg                                                                            <  1:2564124‑M1/138‑1 (MQ=255)
                                                                           aCGGAGGCCTTCCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGGCTCAGATAGAAAAACTGCGCCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACAGGCTg                                                                            >  1:1484778/1‑150 (MQ=255)
                                                                            cttcctgcgctCTGCACTGGCACGTGCCGAAGAAAGGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACTGGCTGa                                                                           <  1:102282‑M1/139‑1 (MQ=255)
                                                                             ttcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACTGGCTGAg                                                                          <  1:879210‑M1/140‑1 (MQ=255)
                                                                              tcctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACTGGCTGAgg                                                                         >  1:1876923‑M1/10‑150 (MQ=255)
                                                                              gAGGCCTTCCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGGCTCAGATAGAAAAACTGCGCCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACAGGCTGAgg                                                                         >  1:2326265/1‑150 (MQ=255)
                                                                               cctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGTGTCGTGAAGTTGAACTGGCTGAGGc                                                                        >  1:3720315‑M1/9‑150 (MQ=255)
                                                                               cctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACTGGCTGAGGc                                                                        >  1:2963692‑M1/9‑150 (MQ=255)
                                                                               cctgcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACTGGCTGAGGc                                                                        >  1:531266‑M1/9‑150 (MQ=255)
                                                                                  gcgctCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACTGGCTGAg                                                                          <  1:1844427‑M1/140‑1 (MQ=255)
                                                                                   cgctCTGCACTGGCACGTGCCGAAGAAAGGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACTGGCTgaggctctg                                                                    >  1:2190408‑M1/5‑146 (MQ=255)
                                                                                    gctCTACACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACTGGCTGAGGCTctgc                                                                   <  1:3430831‑M1/147‑1 (MQ=255)
                                                                                      tCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACTGGCTGAGGCTctgctg                                                                 >  1:819681‑M1/2‑150 (MQ=255)
                                                                                        tGCACTGGCATGTGCCGAAGAAAAGATCGAAGAAGATGAAAGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGACAAACTGCGTCGTGAAGTTGAACTGGCTGCGGCTctgctaaa                                                               >  1:3368938/1‑147 (MQ=25)
                                                                                        tGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGGCTCAGATAGAAAAACTGCGCCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAATAGGCTGAGGCCCTGCTGaa                                                               <  1:1786058/150‑1 (MQ=255)
                                                                                        tGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACTGGCTGAGGCTCTGCTGaa                                                               >  1:2421221/1‑150 (MQ=25)
                                                                                        tGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACTGGCTGAGGCTCTGCTGaa                                                               <  1:1388977/150‑1 (MQ=25)
                                                                                         gCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACTGGCTGAGGCTCTGCTGaaa                                                              <  1:4168199/150‑1 (MQ=25)
                                                                                         gCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACTGGCTGAGGCTCTGCTGaaa                                                              >  1:1950663/1‑150 (MQ=25)
                                                                                         gCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACTGGCTGAGGCTCTGCTGaaa                                                              >  1:247909/1‑150 (MQ=25)
                                                                                          cACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACTGGCTGAGGCTCTGCTGAaac                                                             <  1:1735166/150‑1 (MQ=25)
                                                                                            cTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACTGGCTGAGGCTCTGCTGAaacaa                                                           >  1:1223299/1‑150 (MQ=25)
                                                                                             tGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACTGGCTGAGGCTCTGCTGAaacaac                                                          <  1:1767568/150‑1 (MQ=25)
                                                                                             tGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTATTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACTGGCTGAGGCTCTGCTGAaacaac                                                          <  1:3648368/150‑1 (MQ=25)
                                                                                              ggCACGTGCCGAAGAAAAGATCGAAGAGGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAAATGCCCCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACTGGCTGAGGCTCTGCTGAAACAAcg                                                         >  1:4137460/1‑150 (MQ=21)
                                                                                              ggCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGGCTCAGATAGAAAAACTGCGCCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACAGGCTGAGGCCCTGCTGAAACAAcg                                                         >  1:2222774/1‑150 (MQ=255)
                                                                                              ggCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACTGGCTGAGGCTCTGCTAAAACAAcg                                                         >  1:2833874/1‑150 (MQ=25)
                                                                                                cACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGACTCAGATAGAAAAACTGCACCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAAct                                                                                 >  1:1329504/1‑123 (MQ=255)
                                                                                                          cgaagaGATCGAAGAAGATGAACGGGAAATACAATACTTGCGGGCTTATATAGAAGAACTGCACCGGCTGCTGTTTGGTACCTTTTCTGAAAAACTGCGTCGTGAAGTTGAGCTGGTTGAGGCTCTGCGGAAACAACGTGAACAGGACAg                                             <  1:1001551/145‑1 (MQ=9)
                                                                                                                  tCGAAGAAGATGAACGGGAAATAGAACATCTGCGGGCTCAGATAGAAAAACTGCGCCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACAGGCTGAGGCCCTGCTGAAACAACGCGAACAGGACAGCGATCGtt                                     <  1:963919/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
‑‑‑‑‑‑‑‑‑‑‑‑‑‑CATTGCAGAACTGGAAAGCCAGCTCCGTCAGAAAGACCAGCAACTGAGTCTGGTTGAAGAGACGGAGGCCTTCCTGCACTGGCACGTGCCGAAGAAAAGATCGAAGAAGATGAACGGGAAATAGAACATCTGCGGGCTCAGATAGAAAAACTGCGCCGGATGCTGTTCGGTACCCGTTCTGAAAAACTGCGTCGTGAAGTTGAACAGGCTGAGGCCCTGCTGAAACAACGCGAACAGGACAGCGATCGTTACAGTGGGCGGGAAGACGATCCACAGGTTCCCCGC  >  Xen14_143/1‑285

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑