BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	Xen14_126	2,062	T→C	A92A (GCT→GCC)	Xen14_04726 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Xen14_126	2,062	0	T	C	80.5%	1513.7 / 294.5	844	A92A (GCT→GCC)	Xen14_04726	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (59/105); new base C (373/305); total (432/412)
Fisher's exact test for biased strand distribution p-value = 1.25e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 856 total aligned reads displayed.

AATGATAATGTTATTTTCAAATGGCTCAGACTTTGGCAAAACGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTC‑TTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTGGAAAATCCTTCACCAGAGCTGCTCACGGTGTTGATCCGCGAACTGAGTGGGAGGGGAAGATGATTTC  >  Xen14_126/1913‑2210

aaTGATAATGTTATTTTCAAATGGCTCAGACTTTGGCAAAACGAAGGGCGTATATCGCGACGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGgctgct                                                                                                                                                             <  1:2460535/150‑1 (MQ=255)

             ttttCAAATGGCTCAGACTTTGGCAAAACGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTAAATTATTACCTGTGGAGATAACGCCAGATGAGCCGAAAGAACTGGTGACTGCTC‑TTACTCCGTCtt                                                                                                                                               >  1:4116464/1‑150 (MQ=255)

                   aaTGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCGTCTTTATCCa                                                                                                                                         >  1:237411/1‑150 (MQ=255)

                    aTGGCTCAGACTTTGGCAAAACGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTC‑TTACTCCGTCTTTATCCAc                                                                                                                                        >  1:1850388/1‑150 (MQ=255)

                     tGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCAct                                                                                                                                       <  1:2733559/150‑2 (MQ=255)

                       gCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCActtc                                                                                                                                     >  1:2206898/1‑147 (MQ=255)

                       gCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCActtc                                                                                                                                     >  1:211031/1‑147 (MQ=255)

                       gCTCAGACTTTGGCAAAACGAAGGGCGCATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTC‑TTACTCCGTCTTTATCCAC‑‑‑‑‑C‑Ca                                                                                                                               <  1:170427/150‑1 (MQ=255)

                        cTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCGTCTTTATCCActtcc                                                                                                                                    >  1:3832133/1‑146 (MQ=255)

                        cTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCActtcc                                                                                                                                    <  1:3508110/150‑5 (MQ=255)

                        cTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCActtcc                                                                                                                                    >  1:354634/1‑146 (MQ=255)

                        cTCAGGCTCTGGCAGAACGAAGGGCGCGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCActtcc                                                                                                                                    >  1:417996/1‑146 (MQ=255)

                           aggctctGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCActtccact                                                                                                                                 <  1:846562/144‑8 (MQ=255)

                           aggctctGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCActtccact                                                                                                                                 <  1:4027508/144‑8 (MQ=255)

                              ctctGGCAGAACGAAGGGCGTGTTTCGCGGCGGCTTCCGGTAACGACCTCTTCTNACACTGGCGTTGAATTATTACCTGTAGAAATAANGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCActtccactcag                                                                                                                              >  1:3756525/4‑140 (MQ=255)

                               tctGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCActtccactcaga                                                                                                                             <  1:3472491/148‑12 (MQ=255)

                                  ggCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCActtccactcagacca                                                                                                                          <  1:2404565/150‑15 (MQ=255)

                                  ggCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCActtccactcagacca                                                                                                                          >  1:1091432/1‑136 (MQ=255)

                                  ggCAGAACGAAGGGCATGTTTCGCGGCGTCTTCCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCGTCTTTATCCatttccactcagacca                                                                                                                          >  1:3233278/1‑135 (MQ=255)

                                   gCAAAACGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTC‑TTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAg                                                                                                                   <  1:3591481/150‑1 (MQ=255)

                                        aCGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTC‑TTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCa                                                                                                              >  1:2645716/1‑150 (MQ=255)

                                                 gTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTC‑TTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCa                                                                                                     <  1:2140040/150‑1 (MQ=255)

                                                      tCGCGGCGTCTTCCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgc                                                                                                      <  1:1602565‑M1/150‑35 (MQ=255)

                                                        gcggcgTCTTCCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATCACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaa                                                                                                    >  1:2915308‑M1/1‑114 (MQ=255)

                                                          ggcgTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaagg                                                                                                  <  1:100767‑M1/150‑39 (MQ=255)

                                                          ggcgTCTTCCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCTTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaagg                                                                                                  <  1:3042387‑M1/150‑39 (MQ=255)

                                                              tCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTGTTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTC‑TTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCg                                                                                        <  1:942230/150‑1 (MQ=255)

                                                               cTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTC‑TTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGt                                                                                       <  1:743396/150‑1 (MQ=255)

                                                                tttCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTC‑TTCCTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCCGCAAGGTGGAGTTCCGTc                                                                                      >  1:1908790/1‑150 (MQ=255)

                                                                ttccggtAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaat                                                                                            <  1:279695‑M1/147‑45 (MQ=255)

                                                                     gTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgt                                                                                       >  1:2764924‑M1/1‑101 (MQ=255)

                                                                     gTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgcctgttcctgcaaggtggaattccgt                                                                                       <  1:3656447‑M1/150‑50 (MQ=255)

                                                                      tAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtc                                                                                      <  1:519654‑M1/150‑51 (MQ=255)

                                                                        acgacctcTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcac                                                                                    <  1:3324797‑M1/147‑53 (MQ=255)

                                                                        acgacctcTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcac                                                                                    >  1:952936‑M1/4‑98 (MQ=255)

                                                                         cgacctCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacg                                                                                   >  1:2635734‑M1/3‑97 (MQ=255)

                                                                         cgacctCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacg                                                                                   >  1:3088500‑M1/3‑97 (MQ=255)

                                                                         cgaccacttctGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCACTTCCACTCAGACCAGAGTCAGTGCCAGTTCCTGCAAGGTGGAATTCCGTCACg                                                                                   >  1:368645/7‑150 (MQ=255)

                                                                          gaccTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacgg                                                                                  <  1:204841‑M1/149‑55 (MQ=255)

                                                                           acCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTC‑TTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATg                                                                           >  1:1750692/1‑150 (MQ=255)

                                                                            ccTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGTGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggta                                                                                <  1:3971735‑M1/150‑57 (MQ=255)

                                                                            ccTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggta                                                                                >  1:567887‑M1/1‑94 (MQ=255)

                                                                            ccTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggta                                                                                <  1:1289661‑M1/150‑57 (MQ=255)

                                                                            ccTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggta                                                                                >  1:1173843‑M1/1‑94 (MQ=255)

                                                                              tcttctGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaac                                                                              <  1:1571727‑M1/150‑59 (MQ=255)

                                                                              tcttctGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaac                                                                              >  1:2264670‑M1/1‑92 (MQ=255)

                                                                              tcttctGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaac                                                                              >  1:336835‑M1/1‑92 (MQ=255)

                                                                              tcttctGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaac                                                                              >  1:2994977‑M1/1‑92 (MQ=255)

                                                                               cttctGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaaca                                                                             >  1:801265‑M1/1‑91 (MQ=255)

                                                                               cttctGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaaca                                                                             >  1:4210931‑M1/1‑91 (MQ=255)

                                                                                ttctGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacat                                                                            >  1:1254135‑M1/1‑90 (MQ=255)

                                                                                 tctGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatg                                                                           <  1:2949895‑M1/150‑62 (MQ=255)

                                                                                  ctGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatga                                                                          <  1:3723570‑M1/150‑63 (MQ=255)

                                                                                  ctGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatga                                                                          >  1:447148‑M1/1‑88 (MQ=255)

                                                                                  ctGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCCCttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatga                                                                          >  1:1532049‑M1/1‑88 (MQ=255)

                                                                                  ctGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatga                                                                          >  1:106510‑M1/1‑88 (MQ=255)

                                                                                  ctGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatga                                                                          >  1:2292091‑M1/1‑88 (MQ=255)

                                                                                       acTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctg                                                                     <  1:1473062‑M1/150‑68 (MQ=255)

                                                                                        cTGGCGTTGAATTGTTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctgg                                                                    >  1:4416140‑M1/1‑82 (MQ=255)

                                                                                        cTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctgg                                                                    >  1:3196080‑M1/1‑82 (MQ=255)

                                                                                          ggCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGC‑GGTCATTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaa                                                                  <  1:1953300‑M1/150‑71 (MQ=255)

                                                                                           gCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaa                                                                 >  1:614081‑M1/1‑79 (MQ=255)

                                                                                           gCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaa                                                                 <  1:2533306‑M1/150‑72 (MQ=255)

                                                                                           gCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaa                                                                 <  1:284573‑M1/150‑72 (MQ=255)

                                                                                           gCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacataacgctggaaa                                                                 >  1:675098‑M1/1‑79 (MQ=255)

                                                                                            cGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaa                                                                >  1:2515402‑M1/1‑78 (MQ=255)

                                                                                             gTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaat                                                               <  1:815834‑M1/150‑74 (MQ=255)

                                                                                              ttGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatc                                                              <  1:3796284‑M1/150‑75 (MQ=255)

                                                                                               tGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaagatggaattccgtcacggtaacatgacgctggaaaatcc                                                             >  1:1435046‑M1/1‑75 (MQ=255)

                                                                                                gAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatcca                                                            >  1:2867545‑M1/1‑74 (MQ=255)

                                                                                                 aaTTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccat                                                           >  1:216174‑M1/1‑73 (MQ=255)

                                                                                                  attattACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatc                                                              <  1:4294276‑M1/146‑75 (MQ=255)

                                                                                                  attattACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatc                                                          >  1:3619184‑M1/1‑72 (MQ=255)

                                                                                                  attattACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatc                                                          <  1:399506‑M1/150‑79 (MQ=255)

                                                                                                    tattaCCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgc                                                        >  1:1350736‑M1/1‑70 (MQ=255)

                                                                                                       taCCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccag                                                     >  1:1394426‑M1/1‑67 (MQ=255)

                                                                                                         ccTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagag                                                   >  1:2975856‑M1/1‑65 (MQ=255)

                                                                                                         ccTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagag                                                   >  1:1315521‑M1/1‑65 (MQ=255)

                                                                                                         ccTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagag                                                   <  1:3413092‑M1/150‑86 (MQ=255)

                                                                                                          ctgtagaaataacgccgGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagc                                                  >  1:2580010‑M1/9‑64 (MQ=255)

                                                                                                          ctgtagaaataacgccgGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagc                                                  >  1:701261‑M1/9‑64 (MQ=255)

                                                                                                          ctgtagaaataacgccgGATGAGCAGAAAGAACCTATGGCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagc                                                  >  1:863061‑M1/9‑64 (MQ=255)

                                                                                                            gtagaaataacgcCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctg                                                >  1:4092540‑M1/7‑62 (MQ=255)

                                                                                                            gtagaaataacgcCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctg                                                >  1:2069927‑M1/7‑62 (MQ=255)

                                                                                                               gaaataaCGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgctc                                             >  1:1680361‑M1/4‑59 (MQ=255)

                                                                                                                agaTAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTC‑TTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTGGAAAATCCTTCACCAGAGCTGCTCACGgtgt                                      <  1:1648575/150‑1 (MQ=255)

                                                                                                                 aatAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgctcac                                           <  1:648396‑M1/149‑94 (MQ=255)

                                                                                                                 aatAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgctcac                                           >  1:3289700‑M1/2‑57 (MQ=255)

                                                                                                                      cGCCGGATGAGCCGAAAGAACCGGTGGCTGCTC‑TTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTGGAAAATCCTTCACCAGAGCTGCTCACGGTGTTGATcc                                >  1:1131899/1‑150 (MQ=255)

                                                                                                                       gCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgctcacggtgtt                                     >  1:2900015‑M1/1‑51 (MQ=255)

                                                                                                                       gCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgctcacggtgtt                                     <  1:3132680‑M1/150‑100 (MQ=255)

                                                                                                                       gCCGCATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgctcacggtgtt                                     <  1:3941928‑M1/150‑100 (MQ=255)

                                                                                                                            aTGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgctcacggtgttgatcc                                >  1:200785‑M1/1‑46 (MQ=255)

                                                                                                                            aTGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgctcacggtgttgatcc                                <  1:2562146‑M1/150‑105 (MQ=255)

                                                                                                                                gcagaaaGAACCTGTGGCGGCCA‑TTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctgaaaaatccatcgccagagctgctcacgatgttaatccgcga                            >  1:1209172‑M1/4‑42 (MQ=255)

                                                                                                                                  agaAAGAACCTGTGGCGGCCA‑TTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgctcacggtgttgatccgtgaac                          >  1:2344459‑M1/2‑40 (MQ=255)

                                                                                                                                  agaAAGAACCTGTGGCGGCCA‑TTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgctcacgatgttaatccgcgaac                          >  1:47212‑M1/2‑40 (MQ=255)

                                                                                                                                          ccGGTGGCTGCTC‑TTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTGGAAAATCCTTCACCAGAGCTGCTCACGGTGTTGATCCGCGAACTGAGTGGGAGGGGa            <  1:3874451/150‑1 (MQ=255)

                                                                                                                                                   gctC‑TTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTGGAAAATCCTTCACCAGAGCTGCTCACGGTGTTGATCCGCGAACTGAGTGGGAGGGGAAGATGAttt   >  1:3431005/1‑150 (MQ=255)

                                                                                                                                                   gctC‑TTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTGGAAAATCCTTCACCAGAGCTGCTCACGGTGTTGATCCGCGAACTGAGTGGGAGGGGAAGATGAttt   <  1:892675/150‑1 (MQ=255)

AATGATAATGTTATTTTCAAATGGCTCAGACTTTGGCAAAACGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTC‑TTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTGGAAAATCCTTCACCAGAGCTGCTCACGGTGTTGATCCGCGAACTGAGTGGGAGGGGAAGATGATTTC  >  Xen14_126/1913‑2210

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`