BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	Xen14_126	2,059	T→G	A91A (GCT→GCG)	Xen14_04726 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Xen14_126	2,059	0	T	G	80.4%	1966.0 / inf	845	A91A (GCT→GCG)	Xen14_04726	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (58/107); new base G (372/303); total (432/413)
Fisher's exact test for biased strand distribution p-value = 5.44e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 859 total aligned reads displayed.

GTCAATGATAATGTTATTTTCAAATGGCTCAGACTTTGGCAAAACGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTC‑TTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTGGAAAATCCTTCACCAGAGCTGCTCACGGTGTTGATCCGCGAACTGAGTGGGAGGGGAAGATGA  >  Xen14_126/1910‑2206

gTCAATGATAATGTTATTTTCAAATGGCTCAGACTTTGGCAAAACGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGgct                                                                                                                                                            >  1:441279/1‑150 (MQ=255)

          aTGTTATTTTCAAATGGCTCAGACTTTGGCAAAACGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTC‑TTACTc                                                                                                                                                 >  1:887868/1‑150 (MQ=255)

                ttttCAAATGGCTCAGACTTTGGCAAAACGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTAAATTATTACCTGTGGAGATAACGCCAGATGAGCCGAAAGAACTGGTGACTGCTC‑TTACTCCGTCtt                                                                                                                                           >  1:4116464/1‑150 (MQ=255)

                    cAAATGGCTCAGGCTCTGGCAGAACGAAGGGCATGTTTCGCGGCGTCTTCCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCGTCTTTATc                                                                                                                                       <  1:2336874/150‑1 (MQ=255)

                       aTGGCTCAGGATCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTCCCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCAc                                                                                                                                    >  1:1175331/1‑150 (MQ=255)

                        tGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCAct                                                                                                                                   <  1:2733559/150‑2 (MQ=255)

                          gCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCActtc                                                                                                                                 >  1:668910/1‑147 (MQ=255)

                          gCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCActtc                                                                                                                                 >  1:701174/1‑147 (MQ=255)

                             caggctctGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCActtccac                                                                                                                              <  1:1388817/143‑7 (MQ=255)

                               ggctctGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCActtccactc                                                                                                                            <  1:4466241/145‑9 (MQ=255)

                                 ctctGGCAGAACGAAGGGCGTGTTTCGCGGCGGCTTCCGGTAACGACCTCTTCTNACACTGGCGTTGAATTATTACCTGTAGAAATAANGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCActtccactcag                                                                                                                          >  1:3756529/4‑140 (MQ=255)

                                 cTTTGGCAAAACGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTC‑TTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGactact                                                                                                                    >  1:3725616/1‑150 (MQ=255)

                                   ctGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCActtccactcagac                                                                                                                        <  1:2262907/149‑13 (MQ=255)

                                   ctGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCActtccactcagac                                                                                                                        >  1:3475099/2‑138 (MQ=255)

                                    tGGCAGAACGAAGGGCATGTTTCGCGGCGTCTTCCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCGTCTTTATCCActtccactcagacc                                                                                                                       <  1:3592303/150‑14 (MQ=255)

                                     ggCAGAACGAAGGGCGTGTTTCGCGGTGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCActtccactcagacca                                                                                                                      >  1:220680/1‑136 (MQ=255)

                                     ggCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCActtccactcagacca                                                                                                                      >  1:1091432/1‑136 (MQ=255)

                                     ggCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCActtccactcagacca                                                                                                                      <  1:2404563/150‑15 (MQ=255)

                                     ggCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGACGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCActtccactcagacca                                                                                                                      >  1:3026188/1‑136 (MQ=255)

                                     ggCAGAACGAAGGGCATGTTTCGCGGCGTCTTCCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCGTCTTTATCCatttccactcagacca                                                                                                                      >  1:3233278/1‑135 (MQ=255)

                                      gCAAAACGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTC‑TTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAg                                                                                                               <  1:641315/150‑1 (MQ=255)

                                           aCGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTC‑TTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCa                                                                                                          >  1:2645716/1‑150 (MQ=255)

                                                      atatCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCCGCTC‑TTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAg                                                                                               <  1:3509844/150‑1 (MQ=255)

                                                           gcggcgTCTTCCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATCACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaa                                                                                                >  1:2915308‑M1/1‑114 (MQ=255)

                                                             ggcgTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaagg                                                                                              <  1:100767‑M1/150‑39 (MQ=255)

                                                                 tCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTGTTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTC‑TTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCg                                                                                    <  1:942230/150‑1 (MQ=255)

                                                                  cttccggtaACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaa                                                                                         <  1:3555131‑M1/146‑44 (MQ=255)

                                                                  cttccggtaACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaa                                                                                         >  1:1465981‑M1/5‑107 (MQ=255)

                                                                   ttccggtAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaat                                                                                        <  1:279695‑M1/147‑45 (MQ=255)

                                                                      cGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattcc                                                                                     >  1:2841284‑M1/1‑103 (MQ=255)

                                                                      cGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattcc                                                                                     >  1:2288841‑M1/1‑103 (MQ=255)

                                                                       ggTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccg                                                                                    <  1:742353‑M1/150‑49 (MQ=255)

                                                                        gTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgt                                                                                   >  1:2764924‑M1/1‑101 (MQ=255)

                                                                         tAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtc                                                                                  <  1:3979921‑M1/150‑51 (MQ=255)

                                                                          aacgacctctTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtca                                                                                 >  1:213911‑M1/5‑99 (MQ=255)

                                                                           acgacctcTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcac                                                                                >  1:952936‑M1/4‑98 (MQ=255)

                                                                           acgacctcTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcac                                                                                <  1:3324797‑M1/147‑53 (MQ=255)

                                                                            cgacctCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacg                                                                               >  1:3088500‑M1/3‑97 (MQ=255)

                                                                            cgaccacttctGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCACTTCCACTCAGACCAGAGTCAGTGCCAGTTCCTGCAAGGTGGAATTCCGTCACg                                                                               >  1:368649/7‑150 (MQ=255)

                                                                              acCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggt                                                                             <  1:4006630‑M1/150‑56 (MQ=255)

                                                                               ccTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggta                                                                            >  1:567882‑M1/1‑94 (MQ=255)

                                                                               ccTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggta                                                                            <  1:1289666‑M1/150‑57 (MQ=255)

                                                                               ccTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttccagcaaggtggaattccgtcacggta                                                                            <  1:1749379‑M1/150‑57 (MQ=255)

                                                                                ctctTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaa                                                                           >  1:3365978‑M1/1‑93 (MQ=255)

                                                                                 tcttctGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTC‑TTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACg                                                                    >  1:79386/1‑150 (MQ=255)

                                                                                 tcttctGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaac                                                                          >  1:2994977‑M1/1‑92 (MQ=255)

                                                                                  cttctGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaaca                                                                         >  1:4210931‑M1/1‑91 (MQ=255)

                                                                                   ttctGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacat                                                                        >  1:1254135‑M1/1‑90 (MQ=255)

                                                                                     ctGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatga                                                                      >  1:342713‑M1/1‑88 (MQ=255)

                                                                                     ctGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatga                                                                      >  1:2974955‑M1/1‑88 (MQ=255)

                                                                                     ctGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatga                                                                      >  1:106510‑M1/1‑88 (MQ=255)

                                                                                        acacTGGCGTTGAATTATTACCCGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTC‑TTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTGGaaa                                                             <  1:2456702/150‑1 (MQ=255)

                                                                                         cacTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgct                                                                  >  1:3621583‑M1/1‑84 (MQ=255)

                                                                                          acTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTC‑TTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTGGAAAAt                                                           <  1:3130520/150‑1 (MQ=255)

                                                                                          acTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctg                                                                 <  1:1678135‑M1/150‑68 (MQ=255)

                                                                                           cTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctgg                                                                >  1:3196080‑M1/1‑82 (MQ=255)

                                                                                             ggCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaa                                                              >  1:1906943‑M1/1‑80 (MQ=255)

                                                                                              gCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaa                                                             <  1:2533305‑M1/150‑72 (MQ=255)

                                                                                                gTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGGAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaat                                                           >  1:608594‑M1/1‑77 (MQ=255)

                                                                                                 ttGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatc                                                          >  1:3886997‑M1/1‑76 (MQ=255)

                                                                                                  tGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatcc                                                         <  1:2005704‑M1/150‑76 (MQ=255)

                                                                                                   gAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatcca                                                        <  1:1556439‑M1/150‑77 (MQ=255)

                                                                                                   gAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatcca                                                        >  1:2867545‑M1/1‑74 (MQ=255)

                                                                                                   gAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatcca                                                        >  1:4032457‑M1/1‑74 (MQ=255)

                                                                                                    aaTTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccat                                                       >  1:216173‑M1/1‑73 (MQ=255)

                                                                                                     attattACCTGTGGAGATAACGCCGGATGAGCCGAGAGAACCGGTGGCTGCTC‑TTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTGGAAAATCCTTCACCaga                                                >  1:3944640/1‑150 (MQ=255)

                                                                                                     attattACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTC‑TTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCCGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTGGAAAATCCTTCACCaga                                                >  1:2369947/1‑150 (MQ=255)

                                                                                                     attattACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatc                                                      >  1:1948122‑M1/1‑72 (MQ=255)

                                                                                                     attattACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCGTCTTTATCCACtcccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatc                                                      >  1:2068013‑M1/1‑72 (MQ=255)

                                                                                                       tattaCCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgc                                                    >  1:3803088‑M1/1‑70 (MQ=255)

                                                                                                          taCCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccag                                                 >  1:2945424‑M1/1‑67 (MQ=255)

                                                                                                            ccTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagag                                               <  1:3413092‑M1/150‑86 (MQ=255)

                                                                                                             ctgtagaaataacgccgGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagc                                              <  1:142880‑M1/142‑87 (MQ=255)

                                                                                                             ctgtagaaataacgccgGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagc                                              >  1:2580010‑M1/9‑64 (MQ=255)

                                                                                                              tgtagaaataacgccGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagct                                             >  1:1314180‑M1/8‑63 (MQ=255)

                                                                                                                tagaaataacgCCGGATGAGCAGAAAGAACCTGAGGCGGCCA‑TTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgc                                           <  1:813396‑M1/145‑90 (MQ=255)

                                                                                                                 agaaataacGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgct                                          >  1:497243‑M1/5‑60 (MQ=255)

                                                                                                                  gaaataaCGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgctc                                         <  1:1348257‑M1/147‑92 (MQ=255)

                                                                                                                  gaaataaCGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgctc                                         >  1:4094634‑M1/4‑59 (MQ=255)

                                                                                                                    aatAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgctcac                                       <  1:835870‑M1/149‑94 (MQ=255)

                                                                                                                    aatAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgctcac                                       >  1:3289700‑M1/2‑57 (MQ=255)

                                                                                                                      tAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgctcacgg                                     >  1:1601126‑M1/1‑55 (MQ=255)

                                                                                                                       aaCGCCGGATGAGCCGAAAGAACCGGTGGCTGCTC‑TTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTGGAAAATCCTTCACCAGAGCTGCTCACGGTGTTGAt                              >  1:1642819/1‑150 (MQ=255)

                                                                                                                         cGCCGGATGAGCCGAAAGAACCGGTGGCTGCTC‑TTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTGGAAAATCCTTCACCAGAGCTGCTCACGGTGTTGATcc                            >  1:1131899/1‑150 (MQ=255)

                                                                                                                         cGCCGGATGAGCAGAAAGAACCTGTGGGGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgctcacggtgt                                  >  1:2048289‑M1/1‑52 (MQ=255)

                                                                                                                         cGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgctcacggtgt                                  <  1:1698429‑M1/150‑99 (MQ=255)

                                                                                                                         cGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgctcacggtgt                                  >  1:4197218‑M1/1‑52 (MQ=255)

                                                                                                                         cGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgctcacggtgt                                  <  1:2510336‑M1/150‑99 (MQ=255)

                                                                                                                          gCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgctcacggtgtt                                 >  1:2900013‑M1/1‑51 (MQ=255)

                                                                                                                          gCCGGATGAGCAGAAAGAACCTGTGGC‑GGTCATTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgctcacggtgtt                                 <  1:4314643‑M1/150‑100 (MQ=255)

                                                                                                                              gATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgctcacggtgttgatc                             >  1:3659550‑M1/1‑47 (MQ=255)

                                                                                                                               aTGAGCCGAAAGAACCGGTGGCTGCTC‑TTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTGGAAAATCCTTCACCAGAGCTGCTCACGGTGTTGATCCGCGAAc                      <  1:1525159/150‑1 (MQ=255)

                                                                                                                               aTGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgctcacggtgttgatcc                            <  1:2624951‑M1/150‑105 (MQ=255)

                                                                                                                               aTGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgctcacggtgttgatcc                            <  1:2562145‑M1/150‑105 (MQ=255)

                                                                                                                                tGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCATTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgctcacggtgttgatccg                           <  1:859023‑M1/150‑106 (MQ=255)

                                                                                                                                  agcagaaagAACCTGTGGCGGCCA‑TTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgctcacgatgttaatccgcg                         >  1:1827371‑M1/5‑43 (MQ=255)

                                                                                                                                   gcagaaaGAACCTGTGGCGGCCA‑TTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgctcacgatgttaatccgcga                        <  1:3835739‑M1/147‑109 (MQ=255)

                                                                                                                                   gcagaaaGAACCTGTGGCGGCCA‑TTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctgaaaaatccatcgccagagctgctcacgatgttaatccgcga                        >  1:1209172‑M1/4‑42 (MQ=255)

                                                                                                                                                 tGGCTGCTC‑TTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTGGAAAATCCTTCACCAGAGCTGCTCACGGTGTTGATCCGCGAACTGAGTGGGAGGGGAAgat    <  1:2097098/150‑1 (MQ=255)

                                                                                                                                                    ctgctC‑TTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTGGAAAATCCTTCACCAGAGCTGCTCACGGTGTTg                                <  1:470853/119‑1 (MQ=255)

GTCAATGATAATGTTATTTTCAAATGGCTCAGACTTTGGCAAAACGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTC‑TTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTGGAAAATCCTTCACCAGAGCTGCTCACGGTGTTGATCCGCGAACTGAGTGGGAGGGGAAGATGA  >  Xen14_126/1910‑2206

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`