BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	Xen14_126	2,053	G→T	P89P (CCG→CCT)	Xen14_04726 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Xen14_126	2,053	0	G	T	83.2%	2382.3 / inf	1027	P89P (CCG→CCT)	Xen14_04726	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (65/107); new base T (471/382); total (537/490)
Fisher's exact test for biased strand distribution p-value = 3.76e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1033 total aligned reads displayed.

TGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGACTTTGGCAAAACGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTG‑GCTGCTC‑TTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTGGAAAATCCTTCACCAGAGCTGCTCACGGTGTTGATCCGCGAACTGAGTGGGAGGGGAAG  >  Xen14_126/1906‑2202

  gCGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTg‑g                                                                                                                                                          <  1:2207834/150‑1 (MQ=255)

  gCGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGATGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTg‑g                                                                                                                                                          >  1:1579867/1‑150 (MQ=255)

    gTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGACGGATGAGCAGAAAGAAACTGTG‑gcg                                                                                                                                                        >  1:4146409/1‑149 (MQ=255)

    gTCAATGATAATGTTATTTTCAAATGGCTCAGACTTTGGCAAAACGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTG‑gct                                                                                                                                                        >  1:4008939/1‑150 (MQ=255)

    gTCAATGATAATGTTATTTTCAAATGGCTCAGACTTTGGCAAAACGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTG‑gct                                                                                                                                                        >  1:441281/1‑150 (MQ=255)

     tCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTG‑gcgg                                                                                                                                                       <  1:4195686/150‑3 (MQ=255)

      cAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGTGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTG‑gcggc                                                                                                                                                      <  1:2302310/150‑4 (MQ=255)

      cAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTG‑gcggc                                                                                                                                                      <  1:2467093/150‑4 (MQ=255)

       aaTGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTG‑gcggcc                                                                                                                                                     <  1:3781598/150‑5 (MQ=255)

           aTAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTG‑gcggccattg                                                                                                                                                 >  1:2095067/1‑142 (MQ=255)

            tAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGGAGGGCGGGTTGCGCGGCGTCTGCCGGTAACGACCTCGTCTGACACTGGCGTTGAATTATGACCTTTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTG‑gcggccattgc                                                                                                                                                <  1:2628332/150‑10 (MQ=255)

            tAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTG‑gcggccattac                                                                                                                                                <  1:3908973/150‑10 (MQ=255)

              aTGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTG‑gcggccattgcgc                                                                                                                                              >  1:2513566/1‑139 (MQ=255)

              aTGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCATGTTTCGCGGCGTCTTCCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTG‑gcggccattgcgc                                                                                                                                              >  1:1739776/1‑139 (MQ=255)

              aTGTTATTTTCAAATGGCTCAGACTTTGGCAAAACGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTG‑GCTGCTC‑TTACTc                                                                                                                                             >  1:1869825/1‑150 (MQ=255)

              aTGTTATTTTCAAATGGCTCAGACTTTGGCAAAACGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTG‑GCTGCTC‑TTACTc                                                                                                                                             >  1:887872/1‑150 (MQ=255)

                gTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTG‑gcggccattgcgccg                                                                                                                                            >  1:3499104/1‑137 (MQ=255)

                 ttATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTG‑gcggccattgcgccat                                                                                                                                           >  1:228341/1‑136 (MQ=255)

                  tATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTG‑gcggccattgcgccatc                                                                                                                                          >  1:340753/1‑135 (MQ=255)

                          aaTGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTG‑GCGGCCA‑TTGCGCCGTCTTTATCCa                                                                                                                                 >  1:237411/1‑150 (MQ=255)

                            tGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTG‑GCGGCCA‑TTGCGCCATCTTTATCCAct                                                                                                                               >  1:2034720/1‑149 (MQ=255)

                              gCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTG‑GCGGCCA‑TTGCGCCATCTTTATCCActtc                                                                                                                             >  1:1415984/1‑147 (MQ=255)

                              gCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTG‑GCGGCCA‑TTGCGCCATCTTTATCCActtc                                                                                                                             >  1:255826/1‑147 (MQ=255)

                              gCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCAGGATGAGCAGAAAGAACCTGTG‑GCGGCCA‑TTGCGCCGTCTTTATCCActtc                                                                                                                             >  1:3809173/1‑147 (MQ=255)

                                 caggctctGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTG‑GCGGCCA‑TTGCGCCATCTTTATCCActtccac                                                                                                                          <  1:1216564/143‑7 (MQ=255)

                                  aggctctGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTG‑GCGGCCA‑TTGCGCCATCTTTATCCActtccact                                                                                                                         <  1:846562/144‑8 (MQ=255)

                                     ctctGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCTGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTG‑GCGGCCA‑TTGCGCCATCTTTATCCActtccactcag                                                                                                                      >  1:3540923/4‑140 (MQ=255)

                                     ctctGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTG‑GCGGCCA‑TTGCGCCATCTTTATCCActtccactcag                                                                                                                      <  1:3053153/147‑11 (MQ=255)

                                       ctGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTG‑GCGGCCA‑TTGCGCCATCTTTATCCActtccactcagac                                                                                                                    <  1:2262906/149‑13 (MQ=255)

                                        tGGCAAAACGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTG‑GCTGCTC‑TTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTc                                                                                                             <  1:633069/150‑1 (MQ=255)

                                         ggCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTG‑GCGGCCA‑TTGCGCCATCTTTATCCActtccactcagacca                                                                                                                  >  1:1091432/1‑136 (MQ=255)

                                          gCAAAACGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTG‑GCTGCTC‑TTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAg                                                                                                           <  1:3591481/150‑1 (MQ=255)

                                               aCGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTG‑GCTGCTC‑TTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCa                                                                                                      >  1:2645716/1‑150 (MQ=255)

                                                       cGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTG‑GCTGCTC‑TTACTCCGTCTTTATCCAT‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGc                                                                                              <  1:3087389/150‑1 (MQ=255)

                                                                 ggcgTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTG‑GCGGCCA‑TTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaagg                                                                                          <  1:100767‑M1/150‑39 (MQ=255)

                                                                 ggcgTCTTCCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTG‑GCGGCCA‑TCGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaagg                                                                                          <  1:1490613‑M1/150‑39 (MQ=255)

                                                                     tCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTGTTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTG‑GCTGCTC‑TTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCg                                                                                <  1:942230/150‑1 (MQ=255)

                                                                      cttccggtaACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTG‑GCGGCCA‑TTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaa                                                                                     <  1:3729888‑M1/146‑44 (MQ=255)

                                                                        tccggTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTG‑GCGGCCA‑TTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaatt                                                                                   <  1:297614‑M1/148‑46 (MQ=255)

                                                                         tCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTG‑GCTGCTC‑TTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCAc                                                                            <  1:3240403/150‑1 (MQ=255)

                                                                         tCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTG‑GCTGCTC‑TTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCAc                                                                            >  1:1965706/1‑150 (MQ=255)

                                                                          cGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTG‑GCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattcc                                                                                 >  1:2841284‑M1/1‑103 (MQ=255)

                                                                             tAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTG‑GCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtc                                                                              >  1:2729967‑M1/1‑100 (MQ=255)

                                                                             tAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTG‑GCTGCTC‑TTACTACGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGt                                                                         >  1:3975712/1‑149 (MQ=255)

                                                                              aacgacctctTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTG‑GCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtca                                                                             >  1:213911‑M1/5‑99 (MQ=255)

                                                                               acgacctcTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTG‑GCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcac                                                                            >  1:2915554‑M1/4‑98 (MQ=255)

                                                                                caacCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTG‑GCTGCTC‑TTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACa                                                                     <  1:446725/150‑1 (MQ=255)

                                                                                 aacCTCTTCTGACACCGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTG‑GCGGCCA‑TTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacgg                                                                          >  1:1279738‑M1/1‑96 (MQ=255)

                                                                                  acCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTG‑GCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggt                                                                         >  1:1297745‑M1/1‑95 (MQ=255)

                                                                                     tcttctGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTG‑GCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaac                                                                      >  1:336835‑M1/1‑92 (MQ=255)

                                                                                      cttctGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTG‑GCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaaca                                                                     >  1:801265‑M1/1‑91 (MQ=255)

                                                                                       ttctGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTG‑GCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttccggcaaggtggaattccgtcacggtaacat                                                                    >  1:2792691‑M1/1‑90 (MQ=255)

                                                                                        tctGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCAGGATGAGCAGAAAGAACCTGTG‑GCGGCCA‑TTGCGCCATCTTTATCCACTTCCTCTCAGACCAGAGTCAGTGCCAGTTCCTGCAAGGTGGAATTCCGTCACGGTAACATg                                                                   >  1:2531018/1‑150 (MQ=255)

                                                                                         ctGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTG‑GCTGCTC‑TTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTgg                                                            >  1:2950490/1‑150 (MQ=255)

                                                                                         ctGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTG‑GCGGCCA‑TTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatga                                                                  >  1:1449634‑M1/1‑88 (MQ=255)

                                                                                         ctGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTG‑GCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatga                                                                  >  1:106510‑M1/1‑88 (MQ=255)

                                                                                         ctGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTG‑GCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatga                                                                  >  1:1359729‑M1/1‑88 (MQ=255)

                                                                                          tGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTT‑GCGGCCA‑TTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgac                                                                 <  1:1175508‑M1/150‑64 (MQ=255)

                                                                                          tGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTG‑GCGGCCA‑TTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgac                                                                 >  1:485927‑M1/1‑87 (MQ=255)

                                                                                             cacTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTG‑GCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgct                                                              >  1:3621583‑M1/1‑84 (MQ=255)

                                                                                              acTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTG‑GCTGCTC‑TTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTGGAAAAt                                                       <  1:3130520/150‑1 (MQ=255)

                                                                                               cTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTG‑GCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctgg                                                            >  1:3196080‑M1/1‑82 (MQ=255)

                                                                                                 ggCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTG‑GCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaa                                                          >  1:1694132‑M1/1‑80 (MQ=255)

                                                                                                   cGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAGCCGGTG‑GCTGCTC‑TTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTGGAAAATCCTTc                                                  <  1:4100556/150‑1 (MQ=255)

                                                                                                   cGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTG‑GCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaa                                                        <  1:4214875‑M1/150‑73 (MQ=255)

                                                                                                    gTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTG‑GCTGCTC‑TTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCCCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTGGAAAATCCTTCa                                                 >  1:3306060/1‑150 (MQ=255)

                                                                                                    gTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTG‑GCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaat                                                       <  1:652683‑M1/150‑74 (MQ=255)

                                                                                                     ttGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTG‑GCGGCCA‑TTGCGCCATCTTTATCCACttccactcggaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatc                                                      <  1:519778‑M1/150‑75 (MQ=255)

                                                                                                     ttGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTG‑GCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaat                                                       >  1:694551‑M1/1‑76 (MQ=255)

                                                                                                       gAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTG‑GCGGCCA‑TTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatcca                                                    >  1:2867545‑M1/1‑74 (MQ=255)

                                                                                                       gAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTG‑GCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatcca                                                    >  1:721522‑M1/1‑74 (MQ=255)

                                                                                                        aaTTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTG‑GCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccat                                                   >  1:216174‑M1/1‑73 (MQ=255)

                                                                                                         attattACCTGTGGAGATAACGCCGGATGAGCCGAGAGAACCGGTG‑GCTGCTC‑TTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTGGAAAATCCTTCACCaga                                            >  1:3944640/1‑150 (MQ=255)

                                                                                                         attattACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTG‑GCGGCCA‑TTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatc                                                  >  1:1622830‑M1/1‑72 (MQ=255)

                                                                                                          ttattaCCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTG‑GCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcg                                                 <  1:3692979‑M1/150‑80 (MQ=255)

                                                                                                           tattaCCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTG‑GCTGCTC‑TTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTGGAAAATCCTTCACCAGAgc                                          >  1:3466544/1‑150 (MQ=255)

                                                                                                           tattaCCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTG‑GCGGCCA‑TTGCGCCGTCTTTATCCACttccactcggaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaat                                                       <  1:1670250‑M1/143‑74 (MQ=255)

                                                                                                                ccttgggaggcaaCGTCGGACGAGCCGAAAAAAGCG‑TGTGCTGCTT‑TTGCTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTTTCAGTGCCATCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTGGAAAATCCTTCACCAGAGCTGCTc                                     <  1:4036646/139‑1 (MQ=25)

                                                                                                                 ctgtagaaataacgccgGATGAGCAGACAGAACCTGTG‑GCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagc                                          >  1:1932492‑M1/9‑64 (MQ=255)

                                                                                                                 ctgtagaaataacgccgGATGAGCAGAAAGAACCTGTG‑GCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccaga                                            <  1:300411‑M1/140‑85 (MQ=255)

                                                                                                                 ctgtagaaataacgccgGATGAGCAGAAAGAACCTGTG‑GCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccaga                                            <  1:1322730‑M1/140‑85 (MQ=255)

                                                                                                                 ctgtagaaataacgccgGATGAGCAGAAAGAACCTATG‑GCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagc                                          >  1:863061‑M1/9‑64 (MQ=255)

                                                                                                                    tagaaataacgCCGGATGAACAGAAAGAACCTGTG‑GCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgc                                       <  1:3660767‑M1/145‑90 (MQ=255)

                                                                                                                     agaaataacGCCGGATGAGCAGAAAGAATCTGTG‑GCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgct                                      >  1:572657‑M1/5‑60 (MQ=255)

                                                                                                                     agaaataacGCCGGATGAGCAGAAAGAACCTGTG‑GCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgct                                      >  1:2766009‑M1/5‑60 (MQ=255)

                                                                                                                           aaCGCCGGATGAGCAGAAAGAACCTGTG‑GCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgctcacggt                                >  1:3847111‑M1/1‑54 (MQ=255)

                                                                                                                            aCGCCGGATGAGCAGAAAGAACCTGTG‑GCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgctcacagtg                               <  1:3425137‑M1/150‑98 (MQ=255)

                                                                                                                             cGCCGGATGAGCCGAAAGAACCGGTG‑GCTGCTC‑TTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTGGAAAATCCTTCACCAGAGCTGCTCACGGTGTTGATcc                        >  1:1131899/1‑150 (MQ=255)

                                                                                                                             cGCCGGATGAGCAGAAAGAACCTGTG‑GCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgctcacggtgt                              >  1:818962‑M1/1‑52 (MQ=255)

                                                                                                                              gCCGGATGAGCAGAAAGAACCTGTG‑GCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgctcacggtgtt                             >  1:2900015‑M1/1‑51 (MQ=255)

                                                                                                                              gCCGGATGAGCAGAAAGAACCTGTG‑GCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgctcacggtgtt                             <  1:1792735‑M1/150‑100 (MQ=255)

                                                                                                                              gCCGGATGAGCAGAAAGAACCTGTG‑GCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgctcacggtgtt                             <  1:2065518‑M1/150‑100 (MQ=255)

                                                                                                                              gCCGGATGAGCAGAAAGAACCTGTG‑GCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgctcacggtgtt                             <  1:2430469‑M1/150‑100 (MQ=255)

                                                                                                                              gCCGGATGAGCAGAAAGAACCTGTG‑GC‑GGTCATTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgctcacggtgtt                             <  1:4314643‑M1/150‑100 (MQ=255)

                                                                                                                                  gATGAGCAGAAAGAACCTGTG‑GCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgctcacggtgttgatc                         <  1:3332006‑M1/150‑104 (MQ=255)

                                                                                                                                   aTGAGCCGAAAGAACCGGTG‑GCTGCTC‑TTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTGGAAAATCCTTCACCAGAGCTGCTCACGGTGTTGATCCGCGAAc                  <  1:2572476/150‑1 (MQ=255)

                                                                                                                                   aTGAGCAGAAAGAACCTGTG‑GCGGCCA‑TTGCGCCATCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgctcacggtgttgatcc                        >  1:749253‑M1/1‑46 (MQ=255)

                                                                                                                                         agaAAGAACCTGTG‑GCGGCCA‑TTGCGCCGTCTTTATCCACttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgctcacggtgttgat                          <  1:957747‑M1/141‑103 (MQ=255)

                                                                                                                                            aaGAACCGGTG‑GCTGCTC‑TTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCATCACGGTAACATGACGCTGGAAAATCCTTCACCAGAGCTGCTCACGGTGTTGATCCGCGAACTGAGTGGGa         <  1:1543070/150‑1 (MQ=255)

                                                                                                                                                   ggTG‑GCTGCTC‑TTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTGGAAAATCCTTCACCAGAGCTGCTCACGGTGTTGATCCGCGAACTGAGTGGGAGGGGAAg  >  1:2342867/1‑150 (MQ=255)

TGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGACTTTGGCAAAACGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTG‑GCTGCTC‑TTACTCCGTCTTTATCCAC‑‑‑‑‑C‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTGGAAAATCCTTCACCAGAGCTGCTCACGGTGTTGATCCGCGAACTGAGTGGGAGGGGAAG  >  Xen14_126/1906‑2202

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`