BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	Xen14_126	2,043	C→A	P86Q (CCG→CAG)	Xen14_04726 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Xen14_126	2,043	0	C	A	83.8%	2627.7 / inf	1105	P86Q (CCG→CAG)	Xen14_04726	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (74/105); new base A (504/421); total (578/527)
Fisher's exact test for biased strand distribution p-value = 1.41e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1117 total aligned reads displayed.

TGCTCGAGAAAATGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGACTTTGGCAAAACGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTC‑TTACTCCGTCTTTATCCA‑‑‑C‑‑‑‑‑C‑‑‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTGGAAAATCCTTCACCAGAGCTGCTCACGGTGTTGATCCGCGAACTGAGTG  >  Xen14_126/1894‑2192

   acgtgaAAATGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGaa                                                                                                                                                                >  1:3163461/5‑150 (MQ=255)

   acgtgaAAATGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAAGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGaa                                                                                                                                                                <  1:484600/146‑1 (MQ=255)

     gagaAAATGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGACTTTGGCAAAACGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAg                                                                                                                                                              <  1:2479659/150‑1 (MQ=255)

        aaaaTGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTCCCTGTAGAAATAACACCGGATGAGCAGAAAGAAc                                                                                                                                                           >  1:3121585/1‑150 (MQ=255)

         aaaTGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAAcc                                                                                                                                                          >  1:2202591/1‑150 (MQ=255)

            tGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAAcctgt                                                                                                                                                       >  1:2620481/1‑147 (MQ=255)

               cGTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgc                                                                                                                                                    <  1:3015144/150‑1 (MQ=255)

               cGTCAATGATAATGTTATTTTCAAATGGCTCAGACTTTGGCAAAACGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGGACCGGTGgc                                                                                                                                                    <  1:3918025/150‑1 (MQ=255)

                gTCAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgct                                                                                                                                                   >  1:2309010/1‑150 (MQ=255)

                gTCAATGATAATGTTATTTTCAAATGGCTCAGACTTTGGCAAAACGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGgct                                                                                                                                                   >  1:4008939/1‑150 (MQ=255)

                  cAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggc                                                                                                                                                 <  1:1417066/150‑4 (MQ=255)

                  cAATGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggc                                                                                                                                                 >  1:2942050/1‑147 (MQ=255)

                   aaTGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggcc                                                                                                                                                <  1:963011/150‑5 (MQ=255)

                    aTGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggcca                                                                                                                                               <  1:432943/150‑6 (MQ=255)

                     tGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggccat                                                                                                                                              >  1:1553336/1‑144 (MQ=255)

                     tGATAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggccat                                                                                                                                              >  1:3283757/1‑144 (MQ=255)

                      gATAATGTTATTTTCAAATGGTTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTATAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggccatt                                                                                                                                             >  1:743870/1‑143 (MQ=255)

                        tAATGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggccattgc                                                                                                                                           <  1:1084684/150‑10 (MQ=255)

                          aTGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGTGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCAGGATGAGCAGAAAGAACCTGTGgcggccattgcgc                                                                                                                                         >  1:4148422/1‑139 (MQ=255)

                          aTGTTATTTTCAAATGGCTCAGACTTTGGCAAAACGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTC‑TTACTc                                                                                                                                        >  1:887868/1‑150 (MQ=255)

                           tGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGTGCATGTTTCGCGGCGTCTTCCGGTAACAACCTCTTCTGACACTGGCGGTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggccattgcgcc                                                                                                                                        <  1:3979004/150‑13 (MQ=255)

                           tGTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggccattgcgcc                                                                                                                                        <  1:2705818/150‑13 (MQ=255)

                            gTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGTGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggccattgcgcca                                                                                                                                       >  1:1766620/1‑137 (MQ=255)

                            gTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggccattgcgccg                                                                                                                                       >  1:3499104/1‑137 (MQ=255)

                            gTTATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggccattgcgcca                                                                                                                                       >  1:2339050/1‑137 (MQ=255)

                              tATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggccattgcgccatc                                                                                                                                     >  1:340753/1‑135 (MQ=255)

                              tATTTTCAAATGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGgcggccattgcgccatc                                                                                                                                     >  1:1520257/1‑135 (MQ=255)

                              tATTTTCAAATGGCTCAGACTTTGGCAAAACGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTC‑TTACTCCGTc                                                                                                                                    <  1:4198919/150‑1 (MQ=255)

                                   tcgaaTGGCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCGTCTTTAt                                                                                                                               <  1:3634720/147‑1 (MQ=255)

                                       aTGGCTCAGGATCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTCCCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCA‑‑‑c                                                                                                                        >  1:1175331/1‑150 (MQ=255)

                                          gCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCA‑‑‑cttc                                                                                                                     >  1:257212/1‑147 (MQ=255)

                                          gCTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCA‑‑‑cttc                                                                                                                     >  1:255824/1‑147 (MQ=255)

                                           cTCAGGCTCTGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCA‑‑‑cttcc                                                                                                                    <  1:1852478/150‑5 (MQ=255)

                                                 ctctGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCTGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCA‑‑‑cttccactcag                                                                                                              >  1:3540923/4‑140 (MQ=255)

                                                 ctctGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCGTCTTTATCCA‑‑‑cttccactcag                                                                                                              >  1:84537/4‑140 (MQ=255)

                                                 ctctGGCAGAACGAAGGGCGTGTTTCGCGGCGTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCA‑‑‑cttccactcag                                                                                                              <  1:1712411/147‑11 (MQ=255)

                                                   ctGGCAGAACGAAGGGCGTGTTTCGCGGCCTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCA‑‑‑cttccactcagac                                                                                                            <  1:2118824/149‑13 (MQ=255)

                                                   ctGGCAGAACGAAGGGCATGTTTCGCGGCGTCTTCCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCGTCTTTATCCA‑‑‑cttccactcagac                                                                                                            <  1:2906167/149‑13 (MQ=255)

                                                            cGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTC‑TTACTCCGTCTTTATCCA‑‑‑C‑‑‑‑‑C‑‑‑CAGACTACTGTCAGTGCCAg                                                                                           <  1:4060053/150‑1 (MQ=255)

                                                              aaGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCNGAAAGAACCGGTGGCTGCTC‑TTACTCCGTCTTTATCCA‑‑‑C‑‑‑‑‑C‑‑‑CAGACTACTGTCAGTGCCAGCt                                                                                         <  1:3783454/150‑1 (MQ=255)

                                                                gggCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTC‑TTACTCCGTCTTTATCCA‑‑‑C‑‑‑‑‑C‑‑‑CAGACTACTGTCAGTGCCAGCTcc                                                                                       <  1:3823737/150‑1 (MQ=255)

                                                                       tatCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTC‑TTACTCCGTCTTTATCCA‑‑‑C‑‑‑‑‑C‑‑‑CAGACTACTGTCAGTGCCAGCTCCTGCAAgg                                                                                <  1:1124950/150‑1 (MQ=255)

                                                                           gcggcgTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTC‑TTACTCCGTCTTTATCCA‑‑‑C‑‑‑‑‑C‑‑‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGa                                                                            <  1:3862864/150‑1 (MQ=255)

                                                                            cggcgTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTC‑TTACTCCGTCTTTATCCA‑‑‑C‑‑‑‑‑C‑‑‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAg                                                                           <  1:3600547/150‑1 (MQ=255)

                                                                             ggcgTCTTCCGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCGTCTTTATCCA‑‑‑Cttccactcagaccagagtcagtgccagttcctgcaagg                                                                                  <  1:100767‑M1/150‑39 (MQ=255)

                                                                                 tCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTGTTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTC‑TTACTCCGTCTTTATCCA‑‑‑C‑‑‑‑‑C‑‑‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCg                                                                      <  1:942232/150‑1 (MQ=255)

                                                                                      cGGTAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCA‑‑‑Cttccactcagaccagagtcagtgccagttcctgcaaggtggaattcc                                                                         >  1:2288841‑M1/1‑103 (MQ=255)

                                                                                       ggTAACGACATCTTCTGACACTGGCGTTGAATTATTCCCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCANTTC‑‑‑‑‑CACTCAGACCAGAGTCAGTGCCAGTTCCTGCAAGGTGGAATTCCg                                                                      >  1:2752689/1‑150 (MQ=255)

                                                                                         tAACGACCTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCA‑‑‑Cttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtc                                                                      <  1:2981880‑M1/150‑51 (MQ=255)

                                                                                          aacgacctctTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCA‑‑‑Cttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtca                                                                     >  1:2045693‑M1/5‑99 (MQ=255)

                                                                                           acgacctcTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCA‑‑‑Cttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcac                                                                    >  1:2431709‑M1/4‑98 (MQ=255)

                                                                                           acgacctcTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCA‑‑‑Cttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcac                                                                    <  1:3324799‑M1/147‑53 (MQ=255)

                                                                                              acCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTC‑TTACTCCGTCTTTATCCA‑‑‑C‑‑‑‑‑C‑‑‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATg                                                         >  1:3695508/1‑150 (MQ=255)

                                                                                               ccTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCA‑‑‑Cttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggta                                                                >  1:237696‑M1/1‑94 (MQ=255)

                                                                                               ccTCTTCTGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCA‑‑‑Cttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggta                                                                >  1:813847‑M1/1‑94 (MQ=255)

                                                                                                 tcttctGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTC‑TTACTCCGTCTTTATCCA‑‑‑C‑‑‑‑‑C‑‑‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACg                                                      >  1:79386/1‑150 (MQ=255)

                                                                                                 tcttctGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCA‑‑‑Cttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaac                                                              >  1:336835‑M1/1‑92 (MQ=255)

                                                                                                    tctGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCAGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCA‑‑‑CTTCCTCT‑‑CAGACCAGAGTCAGTGCCAGTTCCTGCAAGGTGGAATTCCGTCACGGTAACATg                                                         >  1:2531018/1‑150 (MQ=255)

                                                                                                     ctGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTC‑TTACTCCGTCTTTATCCA‑‑‑C‑‑‑‑‑C‑‑‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTgg                                                  >  1:4104331/1‑150 (MQ=255)

                                                                                                     ctGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCGTCTTTATCCA‑‑‑Cttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatga                                                          >  1:1449636‑M1/1‑88 (MQ=255)

                                                                                                     ctGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCA‑‑‑Cttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatga                                                          >  1:106510‑M1/1‑88 (MQ=255)

                                                                                                      tGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCGTCTTTATCCA‑‑‑Cttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgac                                                         <  1:2240752‑M1/150‑64 (MQ=255)

                                                                                                      tGACACTGGCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCA‑‑‑Cttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgac                                                         <  1:2273894‑M1/150‑64 (MQ=255)

                                                                                                       gACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTC‑TTACTCCGTCTTTATCCA‑‑‑C‑‑‑‑‑C‑‑‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTGGaa                                                <  1:574316/150‑1 (MQ=255)

                                                                                                          acTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTC‑TTACTCCGTCTTTATCCA‑‑‑C‑‑‑‑‑C‑‑‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTGGAAAAt                                             <  1:441643/150‑1 (MQ=255)

                                                                                                          acTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTC‑TTACTCCGTCTTTATCCA‑‑‑C‑‑‑‑‑C‑‑‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTGGAAAAt                                             <  1:215492/150‑1 (MQ=255)

                                                                                                           cTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTC‑TTACTCCGTCTTTATCCA‑‑‑C‑‑‑‑‑C‑‑‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTGGAAAATc                                            >  1:3083912/1‑150 (MQ=255)

                                                                                                             ggCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTC‑TTACTCCGTCTTTATCCA‑‑‑C‑‑‑‑‑C‑‑‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTGACATGACGCTGGAAAATCCt                                          <  1:289044/150‑1 (MQ=255)

                                                                                                             ggCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCA‑‑‑Cttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaa                                                  >  1:1906943‑M1/1‑80 (MQ=255)

                                                                                                             ggCGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGC‑GGTCATTGCGCCATCTTTATCCA‑‑‑Cttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaa                                                  <  1:1953300‑M1/150‑71 (MQ=255)

                                                                                                               cGTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCA‑‑‑Cttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaa                                                >  1:2515402‑M1/1‑78 (MQ=255)

                                                                                                                gTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCA‑‑‑Cttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaat                                               <  1:1305979‑M1/150‑74 (MQ=255)

                                                                                                                gTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCA‑‑‑Cttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaat                                               <  1:652683‑M1/150‑74 (MQ=255)

                                                                                                                gTTGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCA‑‑‑Cttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaat                                               <  1:13607‑M1/150‑74 (MQ=255)

                                                                                                                 ttGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCA‑‑‑Cttccactcggaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatc                                              <  1:519778‑M1/150‑75 (MQ=255)

                                                                                                                 ttGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCA‑‑‑Cttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatc                                              <  1:3232085‑M1/150‑75 (MQ=255)

                                                                                                                 ttGAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCA‑‑‑Cttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaat                                               >  1:694551‑M1/1‑76 (MQ=255)

                                                                                                                   gAATTATTACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCA‑‑‑Cttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatcca                                            >  1:721522‑M1/1‑74 (MQ=255)

                                                                                                                     attattACCTGTGGAGATAACGCCGGATGAGCCGAGAGAACCGGTGGCTGCTC‑TTACTCCGTCTTTATCCA‑‑‑C‑‑‑‑‑C‑‑‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTGGAAAATCCTTCACCaga                                  >  1:3944640/1‑150 (MQ=255)

                                                                                                                     attattACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCA‑‑‑Cttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgcttgaaaatccatc                                          >  1:2774270‑M1/1‑72 (MQ=255)

                                                                                                                     attattACCTGTAGAAATAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCA‑‑‑Cttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatc                                          >  1:2965776‑M1/1‑72 (MQ=255)

                                                                                                                       tattaCCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTC‑TTACTCCGTCTTTATCCA‑‑‑C‑‑‑‑‑C‑‑‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTGGAAAATCCTTCACCAGAgc                                >  1:3466544/1‑150 (MQ=255)

                                                                                                                             ctgtagaaataacgccgGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCA‑‑‑Cttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcg                                         >  1:2851281‑M1/9‑64 (MQ=255)

                                                                                                                             ctgtagaaataacgccgGATGAGCAGAAAGAACCTATGGCGGCCA‑TTGCGCCATCTTTATCCA‑‑‑Cttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagc                                  >  1:863061‑M1/9‑64 (MQ=255)

                                                                                                                               gtagaaataacgcCGGATGTGCAGAAAGAACCTGTGGCTGCCA‑TTGCGCCGTCTTTATCCA‑‑‑Cttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctg                                >  1:1687338‑M1/7‑62 (MQ=255)

                                                                                                                               gtagaaataacgcCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCA‑‑‑Cttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctg                                >  1:2069928‑M1/7‑62 (MQ=255)

                                                                                                                                 agaaataacGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCA‑‑‑Cttccactcagatcagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgct                              <  1:36712‑M1/146‑91 (MQ=255)

                                                                                                                                  gaaataaCGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCA‑‑‑Cttccactcagaccagagtcagtgccagttcctgcaaggtggaattncgtcacggtaacatgacgctggaaaatccatcgccagagctgctc                             >  1:3737734‑M1/4‑59 (MQ=255)

                                                                                                                                   agaTAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTC‑TTACTCCGTCTTTATCCA‑‑‑C‑‑‑‑‑C‑‑‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTGGAAAATCCTTCACCAGAGCTGCTCACGgtgt                    <  1:1648575/150‑1 (MQ=255)

                                                                                                                                   aaataACGCCGGATGAGCAGAAAGAACTTGTGGCGGCCA‑TTGCGCCATCTTTATCCA‑‑‑Cttccactcagaccagagtcagtgccggttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgctca                            <  1:62306‑M1/148‑93 (MQ=255)

                                                                                                                                      tAACGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCA‑‑‑Cttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgctcacgg                         >  1:1601125‑M1/1‑55 (MQ=255)

                                                                                                                                       aaCGCCGGGTGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCGTCTTTATCCA‑‑‑Cttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgctcacggt                        >  1:1331347‑M1/1‑54 (MQ=255)

                                                                                                                                        aCGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCA‑‑‑Cttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgctcacagtg                       <  1:3425137‑M1/150‑98 (MQ=255)

                                                                                                                                         cGCCGGATGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCA‑‑‑Cttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgctcacggtgt                      >  1:2819013‑M1/1‑52 (MQ=255)

                                                                                                                                          gCCGGATGAGCAGAAAGAACCTGTGGCTGCCA‑TTGCGCCATCTTTATCCA‑‑‑Cttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgctcacggtgtt                     <  1:1280047‑M1/150‑100 (MQ=255)

                                                                                                                                               atgggCCGAAAGAAATGGTGGCTGCGC‑TTACGCCGTCTTTATCCA‑‑‑C‑‑‑‑‑A‑‑‑CAGACGACTGTCAATGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTGGAAAATCCTTCACCAGAGCTGCTCACGGTGTTGATCCGCGAAc        <  1:456855/146‑1 (MQ=25)

                                                                                                                                               aTGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCA‑‑‑Cttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgctcacggtgttgatcc                <  1:1987930‑M1/150‑105 (MQ=255)

                                                                                                                                               aTGAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCATCTTTATCCA‑‑‑Cttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgctcacggtgttgatcc                >  1:424067‑M1/1‑46 (MQ=255)

                                                                                                                                                 gAGCAGAAAGAACCTGTGGCGGCCA‑TTGCGCCGTCTTTATCCA‑‑‑Cttccactcagaccagagtcagtgccagttcctgcaaggtggaattccgtcacggtaacatgacgctggaaaatccatcgccagagctgctcacgatgttaatccgc              <  1:263748‑M1/150‑107 (MQ=255)

                                                                                                                                                   gCCGAAAGAACCGGTGGCTGCTC‑TTACTCCGTCTTTATCCA‑‑‑C‑‑‑‑‑C‑‑‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTGGAAAATCCTTCACCAGAGCTGCTCACGGTGTTGATCCGCGAACTGAg    <  1:1225289/150‑1 (MQ=255)

TGCTCGAGAAAATGGCGTCAATGATAATGTTATTTTCAAATGGCTCAGACTTTGGCAAAACGAAGGGCGTATATCGCGGCGTCTTTCGGTAACAACCTCTTCTGACACTGGCGTTGAATTATTACCTGTGGAGATAACGCCGGATGAGCCGAAAGAACCGGTGGCTGCTC‑TTACTCCGTCTTTATCCA‑‑‑C‑‑‑‑‑C‑‑‑CAGACTACTGTCAGTGCCAGCTCCTGCAAGGTGGAGTTCCGTCACGGTAACATGACGCTGGAAAATCCTTCACCAGAGCTGCTCACGGTGTTGATCCGCGAACTGAGTG  >  Xen14_126/1894‑2192

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`