Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	Xen14_19	57,809	Δ1 bp	intergenic (‑425/+56)	fimA_2 ← / ← Xen14_02204	Type‑1 fimbrial protein, A chain/ISKra4 family transposase ISCep1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Xen14_19	57,809	0	T	.	100.0%	46.2 / NA	12	intergenic (‑425/+56)	fimA_2/Xen14_02204	Type‑1 fimbrial protein, A chain/ISKra4 family transposase ISCep1
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (5/7);  total (5/7)

GTTAGCTTTACATATAGCGGAGGTGATGTGAAATTAATTTACAAGAGAAATAATTTACATATCAAACAGTTAGATGCTTTTTGTCGTTTTTTAATATTTTTATGCTTGAGAAAAAATACGTAACTTATTTATGATATAGACAGTTTGGCCCCAATTGTCTTGTATTTATTTGTTTTTTAACTTTATTATTAATAAGTTAAATCAAGCTTCTTCTCTTTTTAATTTTTCGTTTATG  >  Xen14_19/57664‑57898                                                                                                                                                  |                                                                                          gTTAGCTTTACATATAGCGGAGGTGATGTGAAATTAATTTACAAGAGAAATAATTTACATATCAAACAGTTAGATGCTTTTTGTCGTTTTTTAATATTTTTATGCTTGAGAAAAAATACGTAACTTATTTATGATATAGACAGTT‑GGccc                                                                                      >  1:2956101/1‑150 (MQ=255)   tAGCTTTACATATAGCGGAGGTGATGTGAAATTAATTTACAAGAGAAATAATTTACATATCAAACAGTTAGATGCTTTTTGTCGTTTTTTAATATTTTTATGCTTGAGAAAAAATACGTAACTTATTTATGATATAGACAGTT‑GGCCCCa                                                                                    <  1:331527/150‑1 (MQ=255)   tAGCTTTACATATAGCGGAGGTGATGTGAAATTAATTTACAAGAGAAATAATTTACATATCAAACAGTTAGATGCTTTTTGTCGTTTTTTAATATTTTTATGCTTGAGAAAAAATACGTAACTTATTTATGATATAGACAGTT‑GGCCCCa                                                                                    <  1:331528/150‑1 (MQ=255)                      ggTGATGTGAAATTAATTTACAAGAGAAATAATTTACATATCAAACAGTTAGATGCTTTTTGTCGTTTTTTAATATTTTTATGCTTGAGAAAAAATACGTAACTTATTTATGATATAGACAGTT‑GGCCCCAATTGTCTTGTATTTATTTg                                                                 <  1:1352303/150‑1 (MQ=255)                              gAAATTAATTTACAAGAGAAATAATTTACATATCAAACAGTTAGATGCTTTTCGTCGTTTTTTCATATTTTTATGCTTGAGAAAAAATACGTAACTTATTTATGATATAGACAGTT‑GGCCCCAATTGTCTTGTATTTATTTGTTTTTTaa                                                         >  1:910380/1‑150 (MQ=255)                               aaaTTAATTTACAAGAGAAATAATTTACATATCAAACAGTTAGATGCTTTTTGTCGTTTTTTAATATTTTTATGCTTGAGAAAAAATACGTAACTTATTTATGATATAGACAGTT‑GGCCCCAATTGTCTTGTATTTATTTGTTTTTTAAc                                                        <  1:1665344/150‑1 (MQ=255)                               aaaTTAATTTACAAGAGAAATAATTTACATATCAAACAGTTAGATGCTTTTTGTCGTTTTTTAATATTTTTATGCTTGAGAAAAAATACGTAACTTATTTATGATATAGACAGTT‑GGCCCCAATTGTCTTGTATTTATTTGTTTTTTAAc                                                        <  1:1665345/150‑1 (MQ=255)                                aattaattTACAAGAGAAATAATTTACATATCAAACAGTTAGATGCTTTTTGTCGTTTTTTAATATTTTTATGCTTGAGAAAAAATACGTAACTTATTTATGATATAGACAGTT‑GGCCCCAATTGTCTTGTATTTATTTGTTTTTTAACt                                                       >  1:59575/1‑150 (MQ=255)                                aattaattTACAAGAGAAATAATTTACATATCAAACAGTTAGATGCTTTTTGTCGTTTTTTAATATTTTTATGCTTGAGAAAAAATACGTAACTTATTTATGATATAGACAGTT‑GGCCCCAATTGTCTTGTATTTATTTGTTTTTTAACt                                                       >  1:59578/1‑150 (MQ=255)                                        tACAAGAGAAATAATTTACATATCAAACAGTTAGATGCTTTTTGTCGTTTTTTAATATTTTTATGCTTGAGAAAAAATACGTAACTTATTTATGATATAGACAGTT‑GGCCCCAATTGTCTTGTATGTATTTGTTTTTTAACTttattatt                                               <  1:3940599/150‑1 (MQ=255)                                                                   cAGTTAGATGCTTTTTGTCGTTTTTTAATATTTTTATGCTTGAGAAAAAATACGTAACTTATTTATGATATAGACAGTT‑GGCCCCAATTGTCTTGTATTTATTTGTTTTTTAACTTTATTATTAATAAGTTAAATCAAGCTTCTTCTCtt                    >  1:714732/1‑150 (MQ=255)                                                                                     cGTTTTTTAATATTTTTATGCTTGAGAAAAAATACGTGACTTATTTATGATATAGACAGTT‑GGCCCCAATTGTCTTGTATTTATTTGTTTTTTAACTTTATTATTAATAAGTTAAATCAAGCTTCTTCTCTTTTTAATTTTTCGTTTATg  <  1:2358846/150‑1 (MQ=255)                                                                                                                                                  |                                                                                          GTTAGCTTTACATATAGCGGAGGTGATGTGAAATTAATTTACAAGAGAAATAATTTACATATCAAACAGTTAGATGCTTTTTGTCGTTTTTTAATATTTTTATGCTTGAGAAAAAATACGTAACTTATTTATGATATAGACAGTTTGGCCCCAATTGTCTTGTATTTATTTGTTTTTTAACTTTATTATTAATAAGTTAAATCAAGCTTCTTCTCTTTTTAATTTTTCGTTTATG  >  Xen14_19/57664‑57898

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑